STEPdb: Sub-cellular Topologies of E.coli Polypeptides.

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E.coli BL21(DE3) proteome

(mouse over and click on the one letter topology symbols to see which proteins of this specific subproteome are localized in each location)

Bacterial cell cartoon of sub-cellular locations


Protein ID
(Uniprot)
Accession
(Uniprot)
Gene Name
Order Locus Name
Protein Name
(Uniprot)
Full Name
Symbol
Uniprot
First Level
Secretion
System
First Level
Targeting
Chaperoning
Second Level
Secretion
System
Second Level
Targeting
Chaperoning
E.coli Core
Proteome
(yes/no)
basic proteome
C6EBC6_ECOBD
C6EBC6
 
ECBD_1706
Flagellar motor switch protein FliG
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
 
yes
C6EBE4_ECOBD
C6EBE4
 
ECBD_1725
ABC transporter related
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
 
yes
C6EBF8_ECOBD
C6EBF8
yecI

ftnB

B21_01859 ECBD_1739 ECD_01870
Ferritin Dps family protein (Predicted ferritin-like protein)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
yes
yes
C6EBG0_ECOBD
C6EBG0
araG
B21_01857 ECBD_1741 ECD_01868
ABC transporter related (Fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
yes
yes
C6EBG1_ECOBD
C6EBG1
talB

tal

B21_00008 ECBD_3610 ECD_00008
Transaldolase (EC 2.2.1.2)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
TF
 
 
yes
yes
C6EBG7_ECOBD
C6EBG7
thrA
B21_00002 ECBD_3616 ECD_00002
Aspartate kinase (EC 1.1.1.3) (EC 2.7.2.4) (Bifunctional aspartokinase I/homoserine dehydrogenase I) (EC 1.1.1.3) (EC 2.7.2.4)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
TF
 
 
yes
yes
C6EBI0_ECOBD
C6EBI0
yjjK
B21_04232 ECBD_3629 ECD_04267
ABC transporter related (Fused predicted transporter subunits of ABC superfamily: ATP-binding components)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
TF
 
 
yes
yes
C6EBI2_ECOBD
C6EBI2
radA

sms

B21_04230 ECBD_3631 ECD_04265
DNA repair protein radA
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
yes
yes
C6EBI7_ECOBD
C6EBI7
deoD
B21_04226 ECBD_3636 ECD_04260
Purine nucleoside phosphorylase deoD-type (PNP) (EC 2.4.2.1)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
yes
yes
C6EBI8_ECOBD
C6EBI8
deoB
B21_04225 ECBD_3637 ECD_04259
Phosphopentomutase (EC 5.4.2.7) (Phosphodeoxyribomutase)
Peripheral inner membrane protein facing the cytoplasm
F1
Cytoplasm
 
 
 
 
yes
yes
C6EBJ0_ECOBD
C6EBJ0
deoC
B21_04223 ECBD_3639 ECD_04257
Deoxyribose-phosphate aldolase (EC 4.1.2.4)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
 
yes
C6EBK1_ECOBD
C6EBK1
rsmC
B21_04212 ECBD_3650 ECD_04246
16S ribosomal RNA m2G1207 methyltransferase (EC 2.1.1.52) (rRNA (Guanine-N(2)-)-methyltransferase) (EC 2.1.1.52)
Peripheral inner membrane protein facing the cytoplasm
F1
Cytoplasm
 
 
 
 
yes
yes
C6EBK3_ECOBD
C6EBK3
fhuF
B21_04210 ECBD_3652 ECD_04244
Ferric iron reductase (Ferric iron reductase involved in ferric hydroximate transport)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
yes
yes
C5W2R5_ECOBD
C5W2R5
rlmI
B21_00978
23S rRNA m[5]C1962 methyltransferase monomer, subunit of 23S rRNA m[5]C1962 methyltransferase (EC 2.1.1.-)
Peripheral inner membrane protein facing the cytoplasm
F1
 
 
 
 
 
 
yes
C6EB50_ECOBD
C6EB50
wyz

wzy

B21_01927 ECBD_1623 ECD_01938
O antigen polymerase (Wzy) (Putative uncharacterized protein)
Integral Inner Membrane
B
 
 
 
 
 
 
yes
C6EBE7_ECOBD
C6EBE7
 
ECBD_1728
Putative uncharacterized protein
Integral Inner Membrane
B
 
 
 
 
 
 
yes
C6EB33_ECOBD
C6EB33
 
ECBD_3598 ECD_00020
Putative uncharacterized protein
Periplasmic
G
 
 
 
 
 
 
yes
C5W2A2_ECOBD
C5W2A2
ybl54
B21_00894
Ybl54
Periplasmic
G
 
 
 
 
 
 
yes
C6EBD7_ECOBD
C6EBD7
 
ECBD_1718
Flagellar hook-associated 2 domain protein
Peripheral outer membrane protein facing the extra-cellular space
F4
 
 
 
Flagellum
 
 
yes
C6EBD8_ECOBD
C6EBD8
 
ECBD_1719
Flagellin domain protein
Peripheral outer membrane protein facing the extra-cellular space
F4
 
 
 
Flagellum
 
 
yes
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