entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
YKFM_ECOLIA5A605
ykfM
b4586 integral IM protein (SEC in IM), YkfMIntegral Inner MembraneBBpolyin/outIM (predicted)Membrane -SRPSECnoyesN270.205TMH013.6098-33Ni12-32o52-75i96-119oCytoplasmicMembrane10
YMGJ_ECOLIA5A612
ymgJ
b4594 periplasmic protein (SEC through IM), YmgJWeak signal peptide prediction. Function Unkown.PeriplasmicGIM (predicted)-SECnoyesN240.144CYT0-0.200913-00Yn6-17c22/23oUnknown2
YNCL_ECOLIA5A615
yncL
b4598 JW1439.1 integral IM protein (SEC in IM), YncLIntegral Inner Membrane Small membrane protein detected experimentally. Found to co-fractionate with the inner membrane.PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN270.099CYT0-0.200913-11Ni7-29oCytoplasmicMembrane9
YNEM_ECOLIA5A616
yneM
b4599 JW1527.1 integral IM protein (SEC in IM), Small MgtS Integral Inner Membrane Found to co-fractionate with the inner membrane. Experimentally verified orientation: N(out)-C(in).PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/outN/ACell inner membrane Integral Membrane ProteinSRPSECnoyesN240.123CYT0-0.200913-11Ni7-25oCytoplasmicMembrane9
YDGU_ECOLIA5A617
ydgU
b4601 JW1589 integral IM protein (SEC in IM), YdgUIntegral Inner Membrane Small membrane protein detected experimentally.PMID: 19121005Integral Inner MembraneBBinoutin/outIM (predicted)-SRPSECnoyesN270.097CYT0-0.200913-11Ni7-26oUnknown2
YNHF_ECOLIA5A618
ynhF
b4602 JW1649.1 integral IM protein (SEC in IM), YnhFIntegral Inner Membrane Found to co-fractionate with the inner membrane. Can be inserted in both orientations (Cout-Nin and Cin-Nout).PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/out, out/inIM (predicted)Cell inner membrane -SRPSECnoyesN270.105CYT0-0.200913-11Ni7-28oCytoplasmicMembrane9
DINQ_ECOLIA5A624
dinQ
b4613 integral IM protein (SEC in IM), DinQIntegral Inner MembraneBBinoutin/outIM (predicted)Cell inner membrane -SRPSECnoyesN310.097CYT0-0.200913-11Ni20-38oUnknown2
TISB_ECOLIA5A627
tisB

ysdB

ysdBb4618 JW3649 integral IM protein (SEC in IM), Small toxin TisBIntegral Inner Membrane Potentially toxic protein.PMID: 19121005 PMID: 18761622Integral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane -SRPSECpseudogene (Uniprot), pseudogene (Ecogene)nonoN230.099CYT0-0.200913-11No6-25iUnknown2
YJBT_ECOLIA5A628
yjbT
b4620 periplasmic protein (SEC through IM), YjbTBelongs to the yjbT family.PeriplasmicGN/A-SECnoyesY300.744SpI2915.6556-10Yn15-25c29/30oPeriplasmic9
YTCA_ECOLIA5A630
ytcA
b4622 integral IM protein (SEC in IM), YtcAIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSECnoyesN330.139TMH010.1309-22Yn16-27c32/33o42-61i68-90oCytoplasmicMembrane10
YTHA_ECOLIA8DYQ1
ythA
b4655 JW5769.1 integral IM protein (SEC in IM), YthAIntegral Inner Membrane Small membrane protein detected experimentallyPMID: 19121005Integral Inner MembraneBBinin/outN/ACell inner membrane -SECnoyesN410.12CYT0-0.200913-11Ni21-40oCytoplasmicMembrane9
YKGR_ECOLIC1P5Z8
ykgR
b4671 JW5035.1 integral IM protein (SEC in IM), YkgRIntegral Inner Membrane Found to co-fractionate with the inner membrane. Experimentally verified orientation: N(in)-C(out).PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/outN/ACell inner membrane -SRPSECnoyesN210.096CYT0-0.200913-11Ni12-30oUnknown2
YMIB_ECOLIC1P5Z9
ymiB
b4672 JW1278.1 integral IM protein (SEC in IM), Putative YmiBIntegral Inner MembraneBBinoutin/outN/AMembrane -SRPSECnoyesN280.1CYT0-0.200913-11Ni12-29oUnknown2
RZOQ_ECOLIC1P601
rzoQ
b4689 JW1545.1 IM lipoprotein (SEC through IM), inserted in OM by LOL), Putative RzoQInner Membrane LipoproteinEN/ACell membrane -SECLOLnoyesN290.129SpII2211.4311Pos+2=V00Yn9-20c25/26oUnknown2
YOAK_ECOLIC1P602
yoaK
b4676 JW5292.1 integral IM protein (SEC-YidC in IM), YoaKIntegral Inner Membrane Found to co-fractionate with the inner membrane. Can be inserted in both orientations (Cout-Nin and Cin-Nout). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/out, out/inN/ACell inner membrane -SRPSEC-YidCnoyesN290.152TMH03.55641-11No6-29iCytoplasmicMembrane9
YOAJ_ECOLIC1P603
yoaJ
b4675 JW5292.2 integral IM protein (SEC-YidC in IM), YoaJIntegral Inner Membrane Found to co-fractionate with the inner membrane. Experimentally verified orientation: N(in)-C(out). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/outN/ACell inner membrane -SRPSEC-YidCnoyesN190.098CYT0-0.200913-11No6-23iCytoplasmicMembrane9
YOHP_ECOLIC1P609
yohP
b4679 JW5358.1 integral IM protein (SEC in IM), YohPIntegral Inner Membrane Found to co-fractionate with the inner membrane. Can be inserted in both orientations (Cout-Nin and Cin-Nout).PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutin/out, out/inN/ACell inner membrane -SRPSECnoyesN250.1TMH00.568556-11No6-26iCytoplasmicMembrane9
YPDK_ECOLIC1P610
ypdK
b4680 JW2375.1 integral IM protein (SEC in IM), YpdKIntegral Inner Membrane Found to co-fractionate with the inner membrane.PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutout/inN/ACell inner membrane -SRPSECnoyesN170.106CYT0-0.200913-11No6-22iCytoplasmicMembrane9
SHOB_ECOLIC1P611
shoB

ryfB yphI

ryfB yphIb4687 JW2546.1 integral IM protein (SEC in IM), Small toxin ShoBIntegral Inner Membrane PMID: 19121005 PMID: 18710431Integral Inner MembraneBBinoutin/outN/AMembrane -SRPSECnoyesN130.098CYT0-0.200913-11Ni7-25oCytoplasmicMembrane9
YQFG_ECOLIC1P614
yqfG
b4684 JW5470.1 integral IM protein (SEC in IM), YqfGIntegral Inner Membrane Small protein detected experimentally.PMID: 19121005Integral Inner MembraneB-N/A-SRPSECnoyesN240.133TMH02.01664-10Yn7-17c29/30oCytoplasmicMembrane9
IBSD_ECOLIC1P616
ibsD
b4664 JW3023.1 integral IM protein (SEC in IM), Small toxin IbsDIntegral Inner Membrane Belongs to the toxic protein ibs family. Potentially toxic protein.PMID: 19121005Integral Inner MembraneB-N/AMembrane -SECnoyesN180.098CYT0-0.200913-10Yn4-9c13/14oCytoplasmicMembrane9
PMRR_ECOLIG3MTW7
pmrR
b4703 JW4072.1integral IM protein (SEC in IM), Putative PmrR Integral Inner MembraneBBoutin/outN/ACell inner membrane -SECnoN0.098CYT0-0.200913-11Ni9-26oUnknown2
YPDI_ECOLIO32528
ypdI
b2376 JW2373 OM lipoprotein (SEC through IM, inserted in OM by LOL), YpdIOuter Membrane LipoproteinIN/ACell membrane Outer membrane LipoproteinSECLOLnoyesY240.415SpII1812.5879Pos+2=N10Yn5-16c23/24oUnknown222
YFJS_ECOLIO52982
yfjS
b2636 JW5921 IM lipoprotein (SEC through IM, inserted in OM by LOL), YfjSInner Membrane LipoproteinEN/ACell inner membrane Inner membrane lipoproteinSECLOLProphage Integrase (EcoGene)nonoY210.479SpII2018.7233Pos+2=D00Yn6-17c21/22oUnknown2
GSPB_ECOLIP03825
gspB

pinO pioO pno

pinO pioO pnob3322 JW3284 integral IM protein (SEC in IM), Putative general secretion pathway B GspBIntegral Inner MembraneBBinout/inIM (predicted)Cell membrane Membrane anchoredSRPSECnoyesN430.123TMH03.27755-11No28-51iUnknown2
SODM_ECOLIP00448
sodA
b3908 JW3879 periplasmic protein (SEC through IM), Superoxide dismutase SodAPeriplasmicThe superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are <i>E.coli</i> proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm.PMID: 21854464PeriplasmicGN/ACytoplasmicSECyesyesN510.145CYT0-0.200913-00NiUnknown5
PHSM_ECOLIP00490
malP
b3417 JW5689 integral IM protein (SEC in IM), Maltodextrin phosphorylase MalPIntegral Inner Membrane PMID: 15919657Integral Inner MembraneB-Soluble IM FractionCytoplasmicSECyesyesN540.131CYT0-0.200913-00NiCytoplasmic9
PTM3C_ECOLIP00550
mtlA
b3599 JW3573 integral IM protein (SEC in IM), PTS system mannitol-specific EIICBA component MtlAIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. The signal anchor sequence of MtlA confers novel properties onto nascent chains of OmpA of being co-translationally recognized and targeted to SecY by SRP. PMCID: PMC305875 The signal anchor sequence of MtlA confers novel properties onto nascent chains of OmpA of being co-translationally recognized and targeted to SecY by SRP. Once targeted to SecY, ribosome-associated nascent chains of the hybrid protein, however, remain untranslocated unless SecA is present. These results indicate that SRP and SecA cooperate in a sequential, non-overlapping manner. PMCID: PMC305875PMID: 9008159 PMID: 15919657 PMID: 15919996 PMID: 9260961 PMCID: PMC305875Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN230.115TMH011.9934-88Ni20-42o48-66i78-98o133-154i161-181o213-234i269-292o312-334iCytoplasmicMembrane1026
YGDB_ECOLIP08370
ygdB
b2824 JW5450 periplasmic protein (SEC through IM), YgdBPeriplasmicGN/APeriplasmicSECyesyesN270.147SpI267.55205-10Yn10-23c35/36oUnknown27
PPDB_ECOLIP08371
ppdB

ygdC

ygdCb2825 JW5451 integral IM protein (SEC in IM), Prepilin peptidase-dependent B PpdBIntegral Inner MembraneBBinout/inIM (predicted)Membrane anchoredSRPSECyesyesN360.136CYT0-0.200913-11No13-34iUnknown223
PPDC_ECOLIP08372
ppdC

ygdA

ygdAb2823 JW2791 integral IM protein (SEC in IM), Prepilin peptidase-dependent C PpdCIntegral Inner MembraneBBinout/inIM (predicted)Membrane anchoredSRPSECyesyesN300.122TMH06.3414-11No16-33iUnknown224
PPB_ECOLIP00634
phoA
b0383 JW0374 periplasmic protein (SEC through IM), Alkaline phosphatase PhoAPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 3533724 PMID: 19953543 PMID: 7022451 PMID: 3009404 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAnoyesY220.408SpI213.05238-00Yn5-16c21/22oPeriplasmic1078
LEP_ECOLIP00803
lepB
b2568 JW2552 integral IM protein (SEC-YidC in IM), Signal peptidase I LepBIntegral Inner Membrane Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased. Lep was shown to be targeted to the membrane by the SRP pathway. PMID: 8985168 Disulfide bridge (171 177) PMID: 2551889 PMID: 21778229 PMID: 15919996 PMID: 8985168 Integral Inner MembraneBout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSEC-YidCyesyesN220.131SpII203.04951Pos+2=V20Yn5-16c21/22oCytoplasmicMembrane1027
LSPA_ECOLIP00804
lspA

lsp

lspb0027 JW0025 integral IM protein (SEC in IM), Signal peptidase II LspAIntegral Inner Membrane Catalyzes the removal of signal peptides from prolipoproteins.PMID: 6378662 PMID: 6368552 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN360.135TMH07.83163-44Ni12-31o70-88i100-117o137-157iCytoplasmicMembrane105
ASPG2_ECOLIP00805
ansB
b2957 JW2924 periplasmic protein (SEC through IM), L-asparaginase 2 AnsBPeriplasmicPMID: 387570 PMID: 2407723 PMID: 25403562 PMID: 8434007 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.82SpI2219.7315-10Yn7-17c22/23oPeriplasmic1049
FTSB_ECOLIP0A6S5
ftsB

ygbQ

ygbQb2748 JW2718 integral IM protein (SEC in IM), Cell division FtsBIntegral Inner Membrane PMID: 11972052 PMID: 15165235Integral Inner MembraneBBoutout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECyesyesN230.157CYT0-0.200913-10Yn4-14c18/19oCytoplasmicMembrane925
PSIE_ECOLIP0A7C8
psiE

yjbA

yjbAb4030 JW3990 integral IM protein (SEC in IM), PsiEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN430.143TMH04.00332-44Ni12-35o55-74i86-105o111-128iCytoplasmicMembrane10
YFBV_ECOLIP0A8D9
yfbV
b2295 JW2292 integral IM protein (SEC in IM), UPF0208 YfbVIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.122CYT0-0.200913-22Ni46-64o70-90iCytoplasmicMembrane1038
AMPC_ECOLIP00811
ampC

ampA

ampAb4150 JW4111 periplasmic protein (SEC through IM), Beta-lactamase AmpCPeriplasmicPMID: 6795623PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.854SpI1917.1519-00Yn4-15c19/20oPeriplasmic1026
MALF_ECOLIP02916
malF
b4033 JW3993 integral IM protein (SEC in IM), Maltose/maltodextrin transport system permease MalF Integral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. MalF functions in maltose transport as a 1:1:2 complex with the integral inner membrane protein MalG and the peripheral inner membrane protein MalK. Crystal structure of a catalytic intermediate of the maltose transporter. PMID: 18033289 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 9008159 PMID: 18456666 PMID: 15919996 PMID: 18033289 PMID: 25403562Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRP (exp)SECYfgMnoyesN650.175TMH014.2023-88Ni14-34o40-58i67-91o277-303i315-340o371-392i426-447o484-506iCytoplasmicMembrane10
PBPA_ECOLIP02918
mrcA

ponA

ponAb3396 JW3359 integral IM protein (SEC in IM), Penicillin-binding 1A MrcAIntegral Inner Membrane The formation of a complex between a murein polymerase (PBP1B) and a murein hydrolase (MltA) in the presence of MipA represents a first step in a reconstitution of the hypothetical murein-synthesizing holoenzyme, postulated to be responsible for controlled growth of the stress-bearing sacculus of E. coli. The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Uniprot annotation is Cell inner membrane; Single-pass type II membrane protein, Gene Ontology and Echolocation annotation is Membrane anchored. PMID: 3510188PMID: 10037771 PMID: 21183073 PMID: 15919657 PMID: 3510188Integral Inner MembraneBBoutin/outSoluble IM FractionCell inner membrane Membrane anchored (Experimental)SRPSECyesyesN250.144TMH02.26474-10Yn7-17c24/25oCytoplasmicMembrane104
USPB_ECOLIP0A8S5
uspB

yhiO

yhiOb3494 JW3461 integral IM protein (SEC in IM), Universal stress B UspBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.13TMH00.843073-22No6-24i90-110oCytoplasmicMembrane1057
FLIE_ECOLIP0A8T5
fliE

fla AI flaN

fla AI flaNb1937 JW1921 Flagellar periplasmic peripheral IM protein (Flagellum secreted through IM), hook-basal body complex FliE Peripheral inner membrane protein facing the periplasmMS-ring rod junction proteinPMID: 10809679Peripheral inner membrane protein facing the periplasmF2N/ABacterial flagellum basal body CytoplasmicflagellumFlagellum noyesN410.269CYT0-0.200913-00NiPeriplasmic1062
PBPB_ECOLIP02919
mrcB

pbpF ponB

pbpF ponBb0149 JW0145 integral IM protein (SEC in IM), Penicillin-binding 1B MrcBIntegral Inner Membrane The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Cell inner membrane; Single-pass type II membrane protein. Monoisotopic peripheral; Contains amphipathic a-helix.PMID: 19458048 PMID: 21183073 PMID: 15919657Integral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane; Single-pass type II membrane proteinMembrane anchoredSRPSECyesyesN30.105CYT0-0.200913-11Ni65-86oCytoplasmicMembrane10
LACY_ECOLIP02920
lacY
b0343 JW0334 integral IM protein (SEC in IM), Lactose permease LacYIntegral Inner Membrane Crystal structure of lactose permease indicated that the molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease PMID: 12893935.PMID: 11266632 PMID: 12893935 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN260.125TMH018.848-1112Ni12-34o46-66i75-95o101-122i143-162o168-187i222-239o259-279i291-310o316-337i349-369o381-401iCytoplasmicMembrane10
MELB_ECOLIP02921
melB

mel-4

mel-4b4120 JW4081 integral IM protein (SEC in IM), Melibiose carrier MelBIntegral Inner Membrane PMID: 7703254 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN180.118TMH08.17946-1212Ni12-31o37-57i78-100o106-130i150-171o177-198i236-258o270-290i297-318o324-353i374-397o409-432iCytoplasmicMembrane10
YCCT_ECOLIP0A8X4
yccT
b0964 JW0947 periplasmic protein (SEC through IM), UPF0319 YccTPeriplasmicGN/APeriplasmicSECyesyesY210.531SpI206.4525-00Yn3-14c20/21oUnknown260
ESSD_ECOLIP0A9R2
essD

ybcR

ybcRb0554 JW0543 integral IM protein (SEC in IM), Prophage lysis S homolog EssD Integral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)yesnoN380.193CYT0-0.200913-22Ni7-25o37-58iUnknown214
YOBA_ECOLIP0AA57
yobA
b1841 JW1830 periplasmic protein (SEC through IM), YobAPeriplasmicGN/APeriplasm PeriplasmicSECyesyesY270.511SpI2613.4937-00Yn11-21c26/27oPeriplasmic9
ARAF_ECOLIP02924
araF
b1901 JW1889 periplasmic protein (SEC through IM), L-arabinose-binding periplasmic AraFPeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY240.816SpI2320.0244-00Yn7-18c23/24oPeriplasmic1086
RBSB_ECOLIP02925
rbsB

prlB rbsP

prlB rbsPb3751 JW3730 periplasmic protein (SEC through IM), Ribose import binding RbsB Periplasmic Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 15911532 PMID: 19953543 PMID: 9298646PeriplasmicGSoluble IM FractionPeriplasm Periplasmic (Experimental)SECSKPnoyesY260.839SpI2522.7093-00Yn6-17c25/26oPeriplasmic1093
TONB_ECOLIP02929
tonB

exbA

exbAb1252 JW5195 integral IM protein (SEC in IM), TonBIntegral Inner Membrane PMID: 11967085Integral Inner MembraneBBoutin/outN/ACell inner membrane; Single-pass membrane protein; Periplasmic sideMembrane anchoredSECyesyesN430.176TMH03.06055-01Ni12-32oUnknown2
TOLC_ECOLIP02930
tolC

colE1-i mtcB mukA refI

colE1-i mtcB mukA refIb3035 JW5503 OM b-barrel protein (SEC through IM, inserted in OM by BAM), TolCOuter Membrane b-barrel proteinPart of MdtABC-TolC multidrug efflux transport system = [(TolC)3][MdtC][MdtB][MdtA]. Also part of MacAB-TolC macrolide efflux transport system = [(TolC)3][MacB]2[MacA]. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000).The crystal structure of MacA has been determined at 3.0A resolution. The inner membrane transporter MacB requires the outer membrane factor TolC and the periplasmic adaptor protein MacA to form a functional tripartite complex. Electron microscopic indicates that the MacA hexamer interacts with TolCIn a tip-to-tip manner. Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 12107133 PMID: 19254725 PMID: 21325274 PMID: 16372265 PMID: 16352602 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 10879525 PMID: 15228545 PMID: 16079137 PMID: 21778229 PMID: 20932056 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)SECBSECSKPBAMyesyesY230.712SpI2210.2716-00Yn4-14c22/23oOuterMembrane1015
YACC_ECOLIP0AA95
yacC
b0122 JW0118 periplasmic protein (SEC through IM), YacCPeriplasmicGN/APeriplasmicSECyesyesY230.239SpI2410.6167-00Yn7-17c22/23oUnknown221
YMCE_ECOLIP0AAA5
ymcE

sfa

sfab0991 JW0975 integral IM protein (SEC in IM), YmcEIntegral Inner MembraneBBoutin/outN/ACell membrane Membrane anchoredSRPSECnoyesN460.141TMH04.44945-10Yn15-25c29/30oUnknown2100
ZRAP_ECOLIP0AAA9
zraP

yjaI zra

yjaI zrab4002 JW5546 periplasmic protein (SEC through IM), Zinc resistance-associated protein ZraPPeriplasmicPMID: 9694902 PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.74SpI2628.6968-10Yn7-18c26/27oPeriplasmic10
OMPF_ECOLIP02931
ompF

cmlB coa cry tolF

cmlB coa cry tolFb0929 JW0912 OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpFOuter Membrane b-barrel proteinSubstrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 9298646PMID: 8626309 PMID: 17908933 PMID: 19343722 PMID: 22658749 PMID: 19737520 PMID: 12488465 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 1380671 PMID: 11266632 PMID: 16079137 PMID: 20932056 PMID: 21541338 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)TFSECSKP,SURABAMyesyesY230.883SpI2219.9098-00Yn6-17c22/23oOuterMembrane1018
PHOE_ECOLIP02932
phoE

ompE

ompEb0241 JW0231 OM b-barrel protein (SEC through IM, inserted in OM by BAM), PhoEOuter Membrane b-barrel proteinPMID: 1380671 PMID: 11266632 PMID: 20932056 PMID: 216015Outer Membrane b-barrel proteinHN/ACell outer membrane; Multi-pass membrane proteinOuter Membrane ??-barrel protein (Experimental)SECBAMyesyesY220.841SpI2121.3546-00Yn5-16c21/22oOuterMembrane102
MCP1_ECOLIP02942
tsr

cheD

cheDb4355 JW4318 integral IM protein (SEC in IM), Methyl-accepting chemotaxis I TsrIntegral Inner Membrane PMID: 22380631 PMID: 15919996Integral Inner MembraneBBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN310.141SpI255.90497-21Yn10-21c26/27o192-214iCytoplasmicMembrane1044
YKGJ_ECOLIP0AAL9
ykgJ
b0288 JW0282 periplasmic protein (SEC through IM), YkgJPeriplasmicPeriplasmicGN/APeriplasmicSECnoyesN270.286SpI244.7292-00NiUnknown233
YAIY_ECOLIP0AAP7
yaiY
b0379 JW0370 integral IM protein (SEC in IM), YaiYIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.177TMH0-0.122448-22Ni25-48o68-95iCytoplasmicMembrane1039
YAIZ_ECOLIP0AAQ0
yaiZ
b0380 JW5053 integral IM protein (SEC in IM), YaiZIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN180.115TMH06.70305-22Ni12-32o38-59iCytoplasmicMembrane972
YBAN_ECOLIP0AAR5
ybaN
b0468 JW0457 integral IM protein (SEC in IM), YbaNIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN490.172TMH011.6864-44Ni5-25o31-52i73-91o97-115iCytoplasmicMembrane1015
LAMB_ECOLIP02943
lamB

malB

malBb4036 JW3996 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Maltoporin LamBOuter Membrane b-barrel proteinPotential pubstrate of periplasmic chaperones Skp. Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000). Experimentally verified trimeric beta-barrel. N-terminal region is in periplasm. Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 346375 Disulfide bridges between 47 and 63 PMID: 1988451 Disulfide bond-containing protein PMID: 25664544PMID: 16372265 PMID: 8626309 PMCID: PMC1993877 PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 7824948 PMID: 11266632 PMID: 16079137 PMID: 20932056 PMID: 19953543 PMID: 346375 PMID: 1988451 PMID: 25664544Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)SECSKP,SURA,DEGPBAMyesyesY260.795SpI2520.7922-00Yn8-18c25/26oOuterMembrane1054
KEFC_ECOLIP03819
kefC

trkC

trkCb0047 JW0046 integral IM protein (SEC in IM), Glutathione-regulated potassium-efflux system KefC Integral Inner Membrane PMID: 2046548 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN340.112TMH07.67646-1313No6-24i31-49o55-72i84-111o117-135i147-169o181-202i214-233o239-258i270-290o296-315i327-348o360-378iCytoplasmicMembrane10
MALM_ECOLIP03841
malM

molA

molAb4037 JW3997 periplasmic protein (SEC through IM), Maltose operon periplasmic protein MalMPeriplasmicPMID: 2434655PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.606SpI2624.5925-00Yn7-18c26/27oPeriplasmic10
KDPA_ECOLIP03959
kdpA
b0698 JW0686 integral IM protein (SEC in IM), Potassium-transporting ATPase potassium-binding subunit KdpAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN210.183TMH00.239367-1212Yn5-16c20/21o59-82i94-115o135-155i167-190o253-271i283-301o327-349i356-374o380-398i418-437o484-505i526-548oCytoplasmicMembrane1010
YBAV_ECOLIP0AAR8
ybaV
b0442 JW0432 periplasmic protein (SEC through IM), YbaVPeriplasmicGN/APeriplasmicSECyesyesY260.681SpI2523.157-00Yn7-17c25/26oCytoplasmicMembrane9
YLAC_ECOLIP0AAS0
ylaC
b0458 JW5063 integral IM protein (SEC in IM), YlaCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.106CYT0-0.200913-22Ni36-55o61-79iCytoplasmicMembrane1060
KDPB_ECOLIP03960
kdpB
b0697 JW0685 integral IM protein (SEC in IM), Potassium-transporting ATPase ATP-binding subunit KdpBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN550.195CYT0-0.200913-76Ni35-56o62-82i219-240o252-274i616-636o656-681iCytoplasmicMembrane108
KDPC_ECOLIP03961
kdpC
b0696 JW0684 integral IM protein (SEC in IM), Potassium-transporting ATPase KdpC subunit KdpCIntegral Inner MembraneBBoutin/outN/ACell inner membrane Membrane anchoredSRPSECnoyesN390.2TMH011.3621-10Yn7-18c30/31oCytoplasmicMembrane1018
FIMA1_ECOLIP04128
fimA

pilA

pilAb4314 JW4277 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), Type-1 protein, A chain FimAPeripheral outer membrane protein facing the extra-cellular spaceFim operon (class ??1); Fimbrial Subunit. Experimentally verified signal peptide PMID: 2858471 Disulfide bridge (44 84) Disulfide bond-containing protein PMID: 25664544PMID: 20345943 PMID: 6147250 PMID: 2858471 PMID: 25664544Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUyesyesY240.78SpI2325.7568-10Yn5-16c23/24oExtracellular10
LIVK_ECOLIP04816
livK
b3458 JW3423 periplasmic protein (SEC through IM), Leucine-specific-binding LivKPeriplasmic Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 9298646 PMID: 22030350 PMID: 8061609 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.817SpI2317.7516-00Yn7-18c23/24oPeriplasmic1078
YBFA_ECOLIP0AAU2
ybfA
b0699 JW0688 integral IM protein (SEC in IM), YbfAIntegral Inner MembraneBBinout/inIM (predicted)Membrane anchoredSRPSECnoyesN220.331TMH02.48449-11No12-33iCytoplasmicMembrane912
YBFB_ECOLIP0AAU5
ybfB
b0702 JW0691 integral IM protein (SEC in IM), YbfBIntegral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN290.135TMH015.1783-33No6-24i45-68o80-101iCytoplasmicMembrane1035
YBGE_ECOLIP0AAV0
ybgE
b0735 JW0725 integral IM protein (SEC in IM), YbgEIntegral Inner MembraneBBpolyout/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN410.437TMH03.17076-33No17-34i46-67o73-96iCytoplasmicMembrane1027
YBGS_ECOLIP0AAV6
ybgS
b0753 JW0736 periplasmic protein (SEC through IM), YbgSPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY250.845SpI2432.7676-00Yn6-17c24/25oPeriplasmic9
NLPA_ECOLIP04846
nlpA
b3661 JW3635 IM lipoprotein (SEC through IM, inserted in OM by LOL), NlpAInner Membrane Lipoprotein Proven signal peptidase II substrate.PMID: 15174130Inner Membrane LipoproteinESoluble IM FractionCell inner membrane; Lipid-anchorInner membrane lipoproteinSECLOLyesyesY230.355SpII2321.7987Pos+2=D00Yn10-21c26/27oCytoplasmicMembrane1077
FLIC_ECOLIP04949
fliC

flaF hag

flaF hagb1923 JW1908 Flagellar extra-cellular peripheral OM protein (Flagellum secreted), Flagellin FliCPeripheral outer membrane protein facing the extra-cellular spaceFlameint protein, flagellin. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type fliC, an E. coli strain with a fliC-null mutation produced reduced amounts of OMVs based on both protein and phosphate levels. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 12730325PMID: 24134841 PMID: 21541338 PMID: 12730325Peripheral outer membrane protein facing the extra-cellular spaceF4Soluble IM FractionSecreted Bacterial flagellumCell surface appendage (Experimental)flagellumFlagellumFlanoyesN520.13CYT0-0.200913-00NiExtracellular1080
PTRA_ECOLIP05458
ptrA

ptr

ptrb2821 JW2789 periplasmic protein (SEC through IM), Protease 3 PtrAPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 15502359PMID: 18498364 PMID: 15502359PeriplasmicGN/APeriplasmPeriplasmic(experimental)SECSURAyesyesY240.815SpI2313.0719-00Yn9-18c23/24oPeriplasmic1066
YBHQ_ECOLIP0AAW5
ybhQ
b0791 JW0774 integral IM protein (SEC in IM), YbhQIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.117TMH07.89577-44Ni12-32o38-58i79-96o102-120iCytoplasmicMembrane10
YBIJ_ECOLIP0AAX3
ybiJ
b0802 JW0787 periplasmic protein (SEC through IM), YbiJPeriplasmicGN/APeriplasm PeriplasmicSECyesyesY230.811SpI2221.8351-10Yn7-18c22/23oUnknown2
MCBA_ECOLIP0AAX6
mcbA

ybiM

ybiMb0806 JW5106 periplasmic protein (SEC through IM), McbAPeriplasmicGN/APeriplasm PeriplasmicSECyesyesY230.869SpI2218.838-10Yn4-14c22/23oUnknown2
YBJH_ECOLIP0AAY4
ybjH
b0843 JW0827 periplasmic protein (SEC through IM), YbjHPeriplasmicGN/APeriplasmicSECyesyesY230.874SpI2225.9172-10Yn6-17c22/23oUnknown210
MCP3_ECOLIP05704
trg
b1421 JW1417 integral IM protein (SEC in IM), Methyl-accepting chemotaxis III TrgIntegral Inner Membrane PMID: 22380631 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN400.259TMH07.8524-22Ni20-44o199-222iCytoplasmicMembrane1034
FEPA_ECOLIP05825
fepA

fep feuB

fep feuBb0584 JW5086 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrienterobactin receptor FepAOuter Membrane b-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544PMID: 12624733 PMID: 11266632 PMID: 9886293 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 10529396 PMID: 25664544Outer Membrane b-barrel proteinHN/ACell outer membrane; Multi-pass membrane proteinOuter Membrane ??-barrel protein (Experimental)SECSKPBAMyesyesY230.836SpI2211.5401-00Yn7-17c22/23oOuterMembrane1011
BTUB_ECOLIP06129
btuB

bfe cer dcrC

bfe cer dcrCb3966 JW3938 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Vitamin B12 transporter BtuB Outer Membrane b-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 12652322 PMID: 2982793 PMID: 2687240 PMID: 12595710 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECSKPBAMnoyesY210.828SpI2021.8455-00Yn5-16c20/21oOuterMembrane10
YBJO_ECOLIP0AAZ0
ybjO
b0858 JW0842 integral IM protein (SEC in IM), YbjOIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN180.159TMH04.12536-44Ni21-44o64-87i94-111o131-151iCytoplasmicMembrane1032
YCEK_ECOLIP0AB31
yceK
b1050 JW5151 OM lipoprotein (SEC through IM, inserted in OM by LOL), YceKOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesN310.129SpII155.35698Pos+2=G00Yn3-13c18/19oUnknown2
YCFJ_ECOLIP0AB35
ycfJ
b1110 JW1096 integral IM protein (SEC in IM), YcfJIntegral Inner MembraneB-N/AMembrane Membrane anchoredSRPSECyesyesN360.275SpI238.09735-10Yn6-17c21/22oUnknown2
FTSQ_ECOLIP06136
ftsQ
b0093 JW0091 integral IM protein (SEC in IM), Cell division FtsQIntegral Inner Membrane FtsQ could be localized to the division site in constricting cells. FtsQ insertion requires SecYEG, the transmembrane electrical potential, SecA, and the SRP pathway. PMID: 15546656 YidC plays a role in the membrane integration of the FtsQ protein as the hydrophobic region of FtsQ can be cross-linked to YidC. PMID: 15546656PMID: 2007547 PMID: 9829918 PMID: 9882666 PMID: 11415986 PMID: 11703663 PMID: 11948172 PMID: 15165235 PMID: 17693520 PMID: 15546656Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchoredSRPSECyesyesN410.115TMH01.18618-11Ni25-49oUnknown224
CDH_ECOLIP06282
cdh
b3918 JW3889 periplasmic protein (SEC through IM), CDP-diacylglycerol pyrophosphatase CdhPeriplasmicGSoluble IM FractionCell inner membrane; Single-pass membrane proteinMembrane anchoredSECyesyesN190.264SpI197.43557-10Yn4-14c19/20oCytoplasmicMembrane1055
BTUC_ECOLIP06609
btuC
b1711 JW1701 integral IM protein (SEC-YidC in IM), Vitamin B12 import system permease BtuC Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 11266632 PMID: 12004122 PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN340.278TMH013.5896-99Yn16-29c34/35o58-77i89-109o115-133i145-168o188-206i213-232o238-262i274-297o303-322iCytoplasmicMembrane10
BIRA_ECOLIP06709
birA

bioR dhbB

bioR dhbBb3973 JW3941 nucleoid & integral IM protein (SEC in IM), Bifunctional ligase/repressor BirAIntegral Inner Membrane, NucleoidPMID: 23138451 PMID: 23387333Integral Inner Membrane, NucleoidB, NIM (predicted)CytoplasmicSRPSECyesyesN260.122CYT0-0.200913-02Ni123-139o145-162iCytoplasmic1037
YMGD_ECOLIP0AB46
ymgD
b1171 JW5177 periplasmic protein (SEC through IM), YmgDPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECnoyesY200.656SpI1914.7848-00Yn4-14c19/20oUnknown286
YCHH_ECOLIP0AB49
ychH
b1205 JW1196 integral IM protein (SEC in IM), YchHIntegral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN440.123TMH012.8445-22Ni12-29o41-59iCytoplasmicMembrane973
MRED_ECOLIP0ABH4
mreD
b3249 JW3218 integral IM protein (SEC in IM), Rod shape-determining MreD Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.118TMH015.556-55No6-25i37-54o74-92i104-128o134-152iCytoplasmicMembrane10
CCMD_ECOLIP0ABM5
ccmD

yojM

yojMb2198 JW2186 integral IM protein (SEC in IM), Heme exporter D CcmDIntegral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECnoyesN70.153TMH03.24028-11No12-37iCytoplasmicMembrane966
FHUA_ECOLIP06971
fhuA

tonA

tonAb0150 JW0146 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrichrome transporter/phage receptor FhuAOuter Membrane b-barrel proteinSecB substrate; Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 3079747 Disulfide bridge (351 362;725 731) Disulfide bond-containing protein PMID: 25664544PMID: 16352602 PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 9856937 PMID: 19953543 PMID: 3079747 PMID: 25664544Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECBSECSURA,SKPBAMyesyesY340.805SpI3320.4987-00Yn16-24c33/34oOuterMembrane107
FHUB_ECOLIP06972
fhuB
b0153 JW0149 integral IM protein (SEC in IM), Iron(3+)-hydroxamate import system permease FhuBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN320.415TMH012.8147-1717Yn5-20c38/39o62-79i91-111o117-137i144-167o194-212i224-241o247-265i277-299o305-326i347-369o389-411i423-443o449-468i480-499o567-594i606-623o635-655iCytoplasmicMembrane10
OMPC_ECOLIP06996
ompC

meoA par

meoA parb2215 JW2203 OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpCOuter Membrane b-barrel proteinOuter membrane protein related to resistance to ampicillin and tetracycline; Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 16372265 PMID: 17908933 PMID: 22658749 PMID: 19737520 PMID: 12488465 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 21541338 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane; Multi-pass membrane proteinOuter Membrane ??-barrel protein (Experimental)TFSECSKPBAMyesyesY220.884SpI2121.1188-00Yn5-16c21/22oOuterMembrane106
EXBD_ECOLIP0ABV2
exbD
b3005 JW2973 integral IM protein (SEC in IM), Biopolymer transport ExbDIntegral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane; Single-pass type II membrane proteinMembrane anchoredSRPSECyesyesN420.15TMH00.162291-11No20-41iCytoplasmicMembrane947
TOLR_ECOLIP0ABV6
tolR
b0738 JW0728 integral IM protein (SEC in IM), Tol-Pal system TolR Integral Inner Membrane PMID: 7744737Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchored (Experimental)SRPSECyesyesN370.197TMH00.527259-11Ni21-39oCytoplasmicMembrane923
PNTA_ECOLIP07001
pntA
b1603 JW1595 integral IM protein (SEC in IM), PntAIntegral Inner Membrane PMID: 14660605 PMID: 15919996 PMID: 3525165 PMID: 1932078Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN400.111CYT0-0.200913-54Ni400-418o424-443i455-472o478-499iCytoplasmicMembrane10110
MCP2_ECOLIP07017
tar

cheM

cheMb1886 JW1875 integral IM protein (SEC in IM), Methyl-accepting chemotaxis II TarIntegral Inner Membrane Pole-derived membrane fragments were isolated by affinity capture of vesicles containing the chemotaxis protein, Tar. PMID: 3049536 PMID: 12826616 PMID: 17144658PMID: 22380631 PMID: 15919996 PMID: 3049536 PMID: 12826616 PMID: 17144658Integral Inner MembraneBBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN200.158TMH04.99054-21Yn10-21c26/27o192-212iCytoplasmicMembrane1073
MCP4_ECOLIP07018
tap
b1885 JW1874 integral IM protein (SEC in IM), Methyl-accepting chemotaxis IV TapIntegral Inner Membrane PMID: 22380631 PMID: 15919996Integral Inner MembraneBBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN350.179TMH06.20402-21Yn10-21c34/35o187-209iCytoplasmicMembrane105
YFIB_ECOLIP07021
yfiB
b2605 JW2586 OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative YfiBOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN250.134SpII1818.8761Pos+2=Q00Yn5-16c20/21oOuterMembrane974
FLIL_ECOLIP0ABX8
fliL

cheC1 fla AI fla QI

cheC1 fla AI fla QIb1944 JW1928 integral IM protein (SEC in IM), FliLIntegral Inner MembraneBBoutin/outSoluble IM FractionCell inner membrane Outer membrane LipoproteinSRPSECnoyesN430.268SpII2610.5269Pos+2=A11Ni14-35oCytoplasmicMembrane1035
FLIQ_ECOLIP0AC07
fliQ

flaQ

flaQb1949 JW1933 Flagellar integral IM protein (SEC in IM), biosynthetic FliQIntegral Inner MembraneBBinoutin/inIM (predicted)Cell inner membrane Integral Membrane ProteinflagellumSRPSECnoyesN410.283TMH014.373-21Yn16-35c40/41o56-74iCytoplasmicMembrane1030
HOKC_ECOLIP0ACG4
hokC

gef

gefb4412 JW5002 b0018.1periplasmic protein (SEC through IM), Toxic HokC PeriplasmicPotentially toxic protein.PMID: 19121005 PMID: 21890895PeriplasmicGN/ACell inner membrane PeriplasmicSECnoyesN220.361SpI215.3199-10Yn8-16c21/22oPeriplasmic932
USHA_ECOLIP07024
ushA
b0480 JW0469 periplasmic protein (SEC through IM), UshAPeriplasmicPMID: 9298646 PMID: 25664544PeriplasmicGSoluble IM FractionPeriplasm Periplasmic (Experimental)SECnoyesY260.748SpI2511.3752-00Yn7-18c25/26oPeriplasmic1077
PPA_ECOLIP07102
appA
b0980 JW0963 periplasmic protein (SEC through IM), AppA Periplasmic Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 8387749 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBCyesyesY230.793SpI2213.6965-00Yn3-13c22/23oPeriplasmic1066
PUTP_ECOLIP07117
putP
b1015 JW1001 integral IM protein (SEC in IM), Sodium/proline symporter PutPIntegral Inner Membrane PMID: 16079137 PMID: 15919996 PMID: 3302614Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN280.128TMH013.7134-1212Ni7-26o66-88i127-152o164-186i193-213o233-256i277-296o325-350i371-391o403-422i429-449o455-473iCytoplasmicMembrane10
PSTA_ECOLIP07654
pstA

phoT

phoTb3726 JW3704 integral IM protein (SEC in IM), Phosphate transport system permease PstA Integral Inner Membrane PMID: 3881386 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN140.135TMH014.9057-66Ni29-50o89-111i123-145o151-169i201-222o267-290iCytoplasmicMembrane10
HOKD_ECOLIP0ACG6
hokD

relF

relFb1562 JW1554 periplasmic protein (SEC through IM), Toxic HokD PeriplasmicGN/ACell inner membrane PeriplasmicSECProphage Integrase (EcoGene)nonoN230.157SpI222.62207-10Yn8-19c26/27oPeriplasmic9
YDBJ_ECOLIP0ACW2
ydbJ
b4529 JW5215 OM lipoprotein (SEC through IM, inserted in OM by LOL), YdbJOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesY250.374SpII1617.2226Pos+2=S00Yn3-14c19/20oUnknown224
YDCA_ECOLIP0ACW4
ydcA
b1419 JW1416 periplasmic protein (SEC through IM), YdcAPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECnoyesY210.761SpI2012.9463-00Yn4-15c20/21oUnknown2
FHUD_ECOLIP07822
fhuD
b0152 JW0148 periplasmic protein (TAT-SEC through IM), Iron(3+)-hydroxamate-binding FhuDPeriplasmicTAT secretion system. PMID: 8522527PMID: 17218314 PMID: 8522527 PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECyesyesY310.749SpI308.81274-00Yn12-23c30/31oPeriplasmic10
FIMF_ECOLIP08189
fimF
b4318 JW4281 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimFPeripheral outer membrane protein facing the extra-cellular spaceFim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (38 78) Disulfide bond-containing protein PMID: 25664544PMID: 19820722 PMID: 2890081 PMID: 20345943 PMID: 15372038 PMID: 25664544Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY230.871SpI2217.2366-00Yn6-17c22/23oExtracellular9
FIMG_ECOLIP08190
fimG
b4319 JW4282 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimGPeripheral outer membrane protein facing the extra-cellular spaceFim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (39 77) Disulfide bond-containing protein PMID: 25664544PMID: 19820722 PMID: 2890081 PMID: 20345943 PMID: 15372038 PMID: 25664544Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY240.773SpI2318.1281-10Yn7-18c23/24oExtracellular973
FIMH_ECOLIP08191
fimH
b4320 JW4283 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), Type 1 fimbrin D-mannose specific adhesin FimHPeripheral outer membrane protein facing the extra-cellular spaceFim operon (class ??1); Potential tip-adhesin. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 1971261 PMID: 2900235 PMID: 2890081PMID: 19820722 PMID: 2890081 PMID: 20345943 PMID: 1971261 PMID: 2900235 PMID: 24140205Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbrium Cell surface appendage (Experimental)fimbriaeSECCUnoyesY200.539SpI2114.3197-00Yn4-14c19/20oUnknown419
YEAL_ECOLIP0ACY6
yeaL
b1789 JW1778 integral IM protein (SEC in IM), UPF0756 YeaLIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN250.203TMH08.39191-44Yn4-12c16/17o26-46i53-71o83-100i112-145oCytoplasmicMembrane10
YOHC_ECOLIP0AD17
yohC
b2135 JW5356 integral IM protein (SEC in IM), YohCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN450.135TMH02.03436-55Ni33-53o65-91i103-122o128-149i170-190oCytoplasmicMembrane1016
YPHA_ECOLIP0AD47
yphA
b2543 JW2527 integral IM protein (SEC in IM), YphAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN580.13TMH013.6817-44Ni12-33o53-74i81-100o120-137iCytoplasmicMembrane10
GLPT_ECOLIP08194
glpT
b2240 JW2234 integral IM protein (SEC in IM), Glycerol-3-phosphate transporter GlpTIntegral Inner Membrane PMID: 11266632 PMID: 12893935 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN520.128CYT0-0.200913-1212Ni26-44o64-82i94-113o119-138i159-183o189-208i254-272o292-311i323-342o348-373i385-405o417-437iCytoplasmicMembrane10
CPDB_ECOLIP08331
cpdB
b4213 JW4171 periplasmic protein (SEC through IM), 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase CpdBPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 18498364 PMID: 3005231 PMID: 15911532 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECSURAnoyesY200.787SpI1914.5002-00Yn4-14c19/20oPeriplasmic1082
CRED_ECOLIP08369
creD

cet

cetb4400 JW4363 integral IM protein (SEC-YidC in IM), CreDIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCnoyesN290.114TMH04.58166-65Yn11-22c34/35o295-313i325-343o349-368i375-396o402-420iCytoplasmicMembrane1028
SPPA_ECOLIP08395
sppA
b1766 JW1755 integral IM protein (SEC in IM), Protease 4 SppAIntegral Inner Membrane PMID: 3522590 PMID: 18476724Integral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN480.115TMH06.31524-11Ni21-43oCytoplasmicMembrane1014
OSMB_ECOLIP0ADA7
osmB
b1283 JW1275 OM lipoprotein (SEC through IM), inserted in OM by LOL), Osmotically-inducible B OsmBOuter Membrane LipoproteinProven signal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLnoyesY240.336SpII2322.8573Pos+2=S00Yn9-17c22/23oCytoplasmicMembrane9
ECNA_ECOLIP0ADB4
ecnA
b4410 JW5737 OM lipoprotein (SEC through IM), inserted in OM by LOL), Entericidin A EcnAOuter Membrane LipoproteinSmall membrane protein detected experimentally. Proven pignal peptidase II substrate. PMID: 15174130PMID: 19121005 PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane; Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY230.254SpII1814.5257Pos+2=N00Yn5-16c21/22oCytoplasmicMembrane10
PHOR_ECOLIP08400
phoR

nmpB

nmpBb0400 JW0390 integral IM protein (SEC in IM), Phosphate regulon sensor PhoR PhoRIntegral Inner Membrane PMID: 2187152 PMID: 15919996Integral Inner MembraneBBpolyin/inN/ACell inner membrane Membrane anchoredSRPSECyesyesN240.17TMH020.4877-12Ni12-36o42-59iCytoplasmicMembrane1013
CREC_ECOLIP08401
creC

phoM

phoMb4399 JW4362 integral IM protein (SEC-YidC in IM), Sensor CreCIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN250.159SpI27-0.123855-21Yn7-18c24/25o184-206iCytoplasmicMembrane1016
DACC_ECOLIP08506
dacC
b0839 JW0823 periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacCPeripheral inner membrane protein facing the periplasmPutative amphiphilic alpha-helical anchor at the C-terminusPMID: 8162192. PMID: 9858669 DacC(PBP-6) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. PMID: 9371419PMID: 8162192 PMID: 9858669 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell inner membrane Membrane anchored (Experimental)SECyesyesY280.887SpI2718.4084-00Yn11-22c27/28oCytoplasmicMembrane921
YJJB_ECOLIP0ADD2
yjjB
b4363 JW4327 integral IM protein (SEC in IM), UPF0442 YjjBIntegral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN260.153TMH07.02974-35No6-27i34-52o58-75i87-109o129-151iCytoplasmicMembrane1011
YHHN_ECOLIP0ADI9
yhhN
b3468 JW3433 integral IM protein (SEC in IM), YhhNIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN190.165TMH07.64349-66Yn3-14c18/19o34-64i76-96o102-123i130-152o158-177i189-207oCytoplasmicMembrane10
BCSF_ECOLIP0ADJ5
bcsF

yhjT

yhjTb3537 JW5663 integral IM protein (SEC in IM), Cellulose biosynthesis BcsFIntegral Inner MembraneBBinout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECnoyesN260.211CYT0-0.200913-11No6-25iUnknown268
YIAA_ECOLIP0ADJ8
yiaA
b3562 JW3534 integral IM protein (SEC in IM), YiaAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN220.118TMH07.26426-44Ni12-30o42-62i74-94o106-125iCytoplasmicMembrane1059
CVPA_ECOLIP08550
cvpA

dedE

dedEb2313 JW2310 integral IM protein (SEC in IM), Colicin V production CvpAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN310.117TMH06.39839-44No6-25i32-52o64-87i99-120oCytoplasmicMembrane10
DSDX_ECOLIP08555
dsdX

orf445 yfdA yfdD

orf445 yfdA yfdDb2365 JW2362 integral IM protein (SEC in IM), D-serine transporter DsdXIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECnoyesN330.118TMH011.5143-1212No6-23i30-47o59-77i98-128o140-158i178-198o225-244i256-282o302-322i343-365o385-408i420-444oCytoplasmicMembrane1012
PTV3B_ECOLIP08722
bglF

bglC bglS

bglC bglSb3722 JW3700 integral IM protein (SEC in IM), PTS system beta-glucoside-specific EIIBCA component BglFIntegral Inner Membrane In the absence of ??-glucosidase, associates with BglG to form a pro-form membrane complex that maintains BglG inactive.PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.16CYT0-0.200913-1012Ni100-121o141-160i172-190o210-230i242-263o269-292i299-322o328-347i354-375o381-400i407-424o430-452iCytoplasmicMembrane10
HEMX_ECOLIP09127
hemX
b3803 JW3775 integral IM protein (SEC in IM), HemXIntegral Inner Membrane PMID: 16079137Integral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECnoyesN500.207CYT0-0.200913-11Ni36-57oCytoplasmicMembrane10
YIAW_ECOLIP0ADK4
yiaW
b3587 JW3559 integral IM protein (SEC in IM), YiaWIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.107TMH011.1792-22No6-25i46-66oCytoplasmicMembrane1020
YICN_ECOLIP0ADL3
yicN
b3663 JW5637 integral IM protein (SEC in IM), YicNEcholocation says Membrane anchored, one or two TMH predicted, if signal peptide is also predicted these TMH(s) are not removed as it is probably not cleaved.Integral Inner MembraneB-N/AMembrane anchoredSRPSECyesyesN270.132CYT0-0.200913-10Yn6-17c21/22oUnknown223
YIDG_ECOLIP0ADL6
yidG
b3675 JW3651 integral IM protein (SEC in IM), YidGIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN470.125TMH05.45771-33Ni21-39o45-65i91-116oCytoplasmicMembrane1021
YIDH_ECOLIP0ADM0
yidH
b3676 JW3652 integral IM protein (SEC in IM), YidHIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.135TMH01.23486-33Ni21-39o54-72i93-114oCytoplasmicMembrane1020
OMPT_ECOLIP09169
ompT
b0565 JW0554 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Protease 7 OmpTOuter Membrane b-barrel proteinSecB substrate. Trigger factor-sensitive aggregation. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 9298646PMID: 16352602 PMID: 19737520 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 11566868 PMID: 21541338 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane; Multi-pass membrane proteinOuter Membrane ??-barrel protein (Experimental)SECB,TFSECBAMProphage Integrase (EcoGene)nonoY210.295SpI207.49489-00Yn5-16c20/21oOuterMembrane103
PTW3C_ECOLIP09323
nagE

pstN

pstNb0679 JW0665 integral IM protein (SEC in IM), PTS system N-acetylglucosamine-specific EIICBA component NagEIntegral Inner Membrane PMID: 15919657 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN450.132TMH03.42438-1012Ni12-32o38-61i68-88o94-112i132-153o165-185i192-212o232-252i259-276o282-306i313-331o337-359iCytoplasmicMembrane101
MOTA_ECOLIP09348
motA

flaJ

flaJb1890 JW1879 integral IM protein (SEC in IM), Motility A MotAIntegral Inner Membrane Interacts with flagellar brake protein YcgR in the flagellar basal bodies or FliM or FliG.PMID: 20303158 PMID: 20346719 PMID: 19364805 PMID: 23193105 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.167TMH04.42407-44Yn2-11c16/17o32-49i70-87o171-189i201-220oCytoplasmicMembrane1018
GLPG_ECOLIP09391
glpG
b3424 JW5687 integral IM protein (SEC in IM), Rhomboid protease GlpG Integral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 11266632 PMID: 17051161 PMID: 16216077 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN160.202CYT0-0.200913-66Ni96-115o135-160i172-188o194-212i224-242o248-267iCytoplasmicMembrane10
YIDQ_ECOLIP0ADM4
yidQ
b3688 JW5633 IM lipoprotein (SEC through IM, inserted in OM by LOL), YidQInner Membrane LipoproteinPMID: 11274153Inner Membrane LipoproteinEN/AOuter membrane Lipoprotein(experimental)SECLOLyesyesY270.185SpII2016.396Pos+2=S00Yn5-18c26/27oUnknown2114
YIDX_ECOLIP0ADM6
yidX
b3696 JW5858 OM lipoprotein (SEC through IM, inserted in OM by LOL), YidXOuter Membrane LipoproteinIN/AMembrane Outer membrane LipoproteinSECLOLpseudogene (Ochman & Davalos, 2006)noyesY290.411SpII2320.6632Pos+2=I10Yn11-21c26/27oUnknown254
GLPQ_ECOLIP09394
glpQ
b2239 JW2233 periplasmic protein (SEC through IM), Glycerophosphodiester phosphodiesterase, periplasmic GlpQPeriplasmicPMID: 2829735 PMID: 1851953PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY260.712SpI2517.875-00Yn8-18c25/26oPeriplasmic1039
DEDD_ECOLIP09549
dedD
b2314 JW5378 integral IM protein (SEC in IM), Cell division DedDIntegral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN300.117SpI270.354359-11Ni9-27oUnknown2
ARGT_ECOLIP09551
argT
b2310 JW2307 periplasmic protein (SEC through IM), Lysine/arginine/ornithine-binding periplasmic ArgTPeriplasmicThis periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 2136858 PMID: 19953543 PMID: 9298647 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.595SpI2219.5206-00Yn4-14c22/23oPeriplasmic10
UHPB_ECOLIP09835
uhpB
b3668 JW3643 integral IM protein (SEC in IM), Signal transduction histidine-kinase/phosphatase UhpB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN210.141TMH016.0266-79No6-28i40-60o80-100i112-132o138-162i183-201o207-224i231-249o255-273iCytoplasmicMembrane1019
YGAM_ECOLIP0ADQ7
ygaM
b2672 JW2647 integral IM protein (SEC in IM), YgaMIntegral Inner Membrane YgaM is paralogous to YqjD and binds to ribsomes in a similar manner to YqjD. PMID: 22661687PMID: 22661687Integral Inner MembraneBBinin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECnoyesN400.127CYT0-0.200913-11Ni87-106oUnknown250
YGGE_ECOLIP0ADS6
yggE
b2922 JW2889 periplasmic protein (SEC through IM), YggEPeriplasmicGN/APeriplasmicSECyesyesY220.409SpI2116.0009-00Yn5-16c21/22oPeriplasmic9111
YGIW_ECOLIP0ADU5
ygiW
b3024 JW2992 periplasmic protein (SEC through IM), YgiWPeriplasmicSecB substrate. PMID: 9298646PMID: 16352602 PMID: 9298646PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECBSECyesyesY210.877SpI2026.1756-00Yn4-15c20/21oUnknown228
UHPC_ECOLIP09836
uhpC
b3667 JW3642 integral IM protein (SEC in IM), sensor UhpC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN520.143CYT0-0.200913-1212Ni26-45o65-84i96-114o120-143i155-179o185-203i245-265o285-308i320-338o344-365i377-401o407-431iCytoplasmicMembrane10
UBIB_ECOLIP0A6A0
ubiB

aarF yigQ yigR yigS

aarF yigQ yigR yigSb3835 JW3812 integral IM protein (SEC in IM), Probable kinase UbiB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN500.114CYT0-0.200913-22Ni501-518o524-542iCytoplasmicMembrane1017
CLSA_ECOLIP0A6H8
clsA

cls nov yciJ

cls nov yciJb1249 JW1241 integral IM protein (SEC in IM), Cardiolipin synthase A ClsAIntegral Inner Membrane In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. PMID: 22988102PMID: 15919996 PMID: 22988102Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN220.149TMH05.41966-22No6-25i37-58oCytoplasmicMembrane103
CYSZ_ECOLIP0A6J3
cysZ
b2413 JW2406 integral IM protein (SEC-YidC in IM), Sulfate transporter CysZ Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 15919996PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSEC-YidCyesyesN180.111TMH05.82324-45Ni31-51o71-92i143-163o169-188i209-242oCytoplasmicMembrane1019
LPTA_ECOLIP0ADV1
lptA

yhbN

yhbNb3200 JW3167 periplasmic protein (SEC through IM), polysaccharide export system LptA PeriplasmicPMID: 17056748 PMID: 18480051 PMID: 9298646PeriplasmicGN/APeriplasm Periplasmic(experimental)SECyesyesY280.838SpI2724.4302-00Yn12-23c27/28oUnknown223
YHHA_ECOLIP0ADX7
yhhA
b3448 JW3413 periplasmic protein (SEC through IM), YhhAPeriplasmicGN/APeriplasmicSECyesyesY180.377SpI1714.5283-00Yn4-11c17/18oUnknown2107
YQAE_ECOLIP0AE42
yqaE
b2666 JW2641 integral IM protein (SEC in IM), UPF0057 YqaEIntegral Inner MembraneBBinoutout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN270.14TMH06.73627-21No23-48iCytoplasmicMembrane10
CREA_ECOLIP0AE91
creA

yjjD

yjjDb4397 JW4360 periplasmic protein (SEC through IM), CreAPeriplasmic Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 25664544PeriplasmicGN/APeriplasmicSECDSBAyesyesY220.8SpI2114.9398-00Yn6-16c21/22oUnknown2
DSBB_ECOLIP0A6M2
dsbB

roxB ycgA

roxB ycgAb1185 JW5182 integral IM protein (SEC-YidC in IM), Disulfide bond formation B DsbBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC PMID: 22108384. The crystal structure revealed a transmembrane (TM) four-helix-bundle structure of DsbBPMID: 18406599. DsbB oxidizes the periplasmic protein DsbA, which in turn oxidizes the cysteines in other periplasmic proteins to make disulfide bondsPMID: 8430071. Gene fusion approaches were used to show that (i) DsbB is a membrane protein which spans the membrane four times and (ii) both the N- and C-termini of the protein are in the cytoplasmPMID: 7957076. The structure of DsbB revealed four transmembrane helices and one short horizontal helix juxtaposed with Cys130 in the mobile periplasmic loopPMID: 17110337. Disulfide bridge (41 44;104 130) PMID: 10064586PMID: 18406599 PMID: 11266632 PMID: 17110337 PMID: 22108384 PMID: 8430071 PMID: 7957076 PMID: 15919996 PMID: 10064586Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN250.286TMH04.8983-44Ni12-33o45-64i71-89o145-166iCytoplasmicMembrane1015
ENO_ECOLIP0A6P9
eno
b2779 JW2750 cytoplasmic peripheral IM protein facing the cytoplasm & peripheral OM protein facing the extra-cellular space (SEC through IM), Enolase EnoCytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceWhen modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. PMID: 8610017 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 17242352 PMID: 8610017 PMID: 15003462 PMID: 18337249 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceA, F1, F4Soluble IM FractionCytoplasm; cytoskeleton Secreted Cell surface Cytoplasmic (Experimental)SECyesyesN420.139CYT0-0.200913-00NiCytoplasmic9100
FLGH_ECOLIP0A6S0
flgH

fla FVIII flaY

fla FVIII flaYb1079 JW5153 Flagellar OM lipoprotein (SEC through IM), inserted in OM by LOL), L-ring FlgHOuter Membrane Lipoprotein Proven pignal peptidase II substrate. L ring protein. PMID: 15174130PMID: 15174130 PMID: 12631703 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)flagellumSECLOLyesyesY240.235SpII2117.0713Pos+2=A00Yn14-25c33/34oOuterMembrane1024
YJIG_ECOLIP0AEH8
yjiG
b4329 JW4292 integral IM protein (SEC in IM), YjiGIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN360.157TMH02.83449-34Ni21-39o59-77i89-110o130-152iCytoplasmicMembrane102
YIIR_ECOLIP0AF34
yiiR
b3921 JW3892 integral IM protein (SEC in IM), YiiRIntegral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN380.144TMH05.06913-44Ni20-38o44-63i75-93o99-121iCytoplasmicMembrane1049
YIJD_ECOLIP0AF40
yijD
b3964 JW3936 integral IM protein (SEC in IM), YijDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN270.345TMH08.09717-43Yn10-21c26/27o36-51i63-83o89-108iCytoplasmicMembrane1021
FLGI_ECOLIP0A6S3
flgI

fla FIX flaM

fla FIX flaMb1080 JW1067 Flagellar periplasmic protein (SEC through IM), P-ring FlgIPeriplasmicP ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 14726535 PMID: 12631703 PMID: 15838024 PMID: 19953543 PMID: 12730325PeriplasmicGN/APeriplasm Bacterial flagellum basal bodyPeriplasmic (Experimental)flagellumSRP (exp)SECDSBAFlanoyesY200.783SpI1915.6262-00Yn4-14c19/20oPeriplasmic1063
MRAY_ECOLIP0A6W3
mraY

murX

murXb0087 JW0085 integral IM protein (SEC in IM), Phospho-N-acetylmuramoyl-pentapeptide-transferase MraYIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN460.151CYT0-0.200913-1010No20-40i73-90o96-114i134-152o172-193i200-219o239-256i263-284o290-311i338-357oCytoplasmicMembrane10
YCIB_ECOLIP0A710
yciB

ispZ

ispZb1254 JW1246 integral IM protein (SEC in IM), Probable intracellular septation A YciBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN250.173TMH07.58982-55No20-39i51-68o74-96i117-139o151-170iCytoplasmicMembrane10
KDGT_ECOLIP0A712
kdgT
b3909 JW5560 integral IM protein (SEC in IM), 2-keto-3-deoxygluconate permease KdgTIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN670.134TMH012.8678-1010Ni12-29o35-57i78-97o103-127i139-164o170-187i194-211o223-243i255-274o286-309iCytoplasmicMembrane10
MSCL_ECOLIP0A742
mscL

yhdC

yhdCb3291 JW3252 integral IM protein (YidC in IM), Large-conductance mechanosensitive channel MscLIntegral Inner Membrane PMID: 17113597 PMID: 22108384 PMID: 8890153 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPYidCyesyesN390.143CYT0-0.200913-22Ni21-52o72-96iCytoplasmicMembrane1066
YJBE_ECOLIP0AF45
yjbE
b4026 JW3986 periplasmic protein (SEC through IM), YjbEPeriplasmicGN/APeriplasmicSECnoyesY220.853SpI2125.8362-00Yn4-15c21/22oExtracellular9
YJCH_ECOLIP0AF54
yjcH
b4068 JW4029 integral IM protein (SEC in IM), YjcHIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN480.119TMH04.65174-22Ni25-51o63-84iCytoplasmicMembrane1054
YJET_ECOLIP0AF73
yjeT
b4176 JW4134 integral IM protein (SEC in IM), YjeTIntegral Inner MembraneBBinoutin/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN270.108TMH04.78812-21Yn3-13c17/18o41-60iCytoplasmicMembrane919
YJFL_ECOLIP0AF80
yjfL
b4184 JW4142 integral IM protein (SEC in IM), UPF0719 YjfLIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.123TMH08.65955-44No6-28i49-69o75-97i109-128oCytoplasmicMembrane1019
RSXA_ECOLIP0A766
rsxA

rnfA ydgL

rnfA ydgLb1627 JW1619 integral IM protein (SEC in IM), Ion-translocating oxidoreductase complex subunit A RsxAIntegral Inner Membrane PMID: 15919996 PMID: 24688744Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane Protein(experimental)SRPSECyesyesN210.118TMH07.31415-66No6-26i38-59o65-87i99-122o134-155i167-191oCytoplasmicMembrane10
MNTH_ECOLIP0A769
mntH

yfeP

yfePb2392 JW2388 integral IM protein (SEC in IM), Divalent metal cation transporter MntHIntegral Inner Membrane PMID: 14607838 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN280.129TMH09.54648-1111Ni21-40o52-75i95-116o122-144i156-176o196-216i237-263o283-313i325-343o349-370i391-410oCytoplasmicMembrane10
GPMB_ECOLIP0A7A2
gpmB

ytjC

ytjCb4395 JW4358 integral IM protein (SEC in IM), Probable phosphoglycerate mutase GpmB Large potential TM 146-168aaIntegral Inner MembraneBBinoutout/inN/AMembrane anchoredSRPSECnoyesN170.117CYT0-0.200913-11No146-168iCytoplasmic956
DCTA_ECOLIP0A830
dctA
b3528 JW3496 integral IM protein (SEC-YidC in IM), Aerobic C4-dicarboxylate transport protein DctAIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCnoyesN190.136TMH05.74612-88Ni9-27o47-64i76-99o141-164i184-208o220-242i326-344o350-373iCytoplasmicMembrane10
TATE_ECOLIP0A843
tatE

ybeC

ybeCb0627 JW0622 integral IM protein (SEC in IM), Sec-independent translocase TatE Integral Inner Membrane TatA paralog TatE can substitute for TatA and support translocation of Tat substrates including AmiA, AmiC, and TorA.PMID: 22190680Integral Inner MembraneBBoutout/inIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN430.118CYT0-0.200913-10Yn9-20c29/30oCytoplasmicMembrane9127
YJFN_ECOLIP0AF82
yjfN
b4188 JW5742 periplasmic protein (SEC through IM), YjfNPeriplasmicGN/APeriplasm PeriplasmicSECyesyesN200.293SpI217.51366-00Yn4-15c23/24oUnknown2104
YJFY_ECOLIP0AF86
yjfY
b4199 JW4157 periplasmic protein (SEC through IM), YjfYPeriplasmicGN/APeriplasm PeriplasmicSECyesyesY210.833SpI2015.6931-00Yn5-15c20/21oUnknown293
PSIF_ECOLIP0AFM4
psiF
b0384 JW5054 periplasmic protein (SEC through IM), Phosphate starvation-inducible PsiF Periplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY220.376SpI2113.709-10Yn5-16c21/22oUnknown244
TOLB_ECOLIP0A855
tolB
b0740 JW5100 periplasmic & peripheral OM protein (SEC through IM), Tol-Pal system TolB Periplasmic, Peripheral outer membrane protein facing the periplasmSecB substrate. Might be is co-translationally translocated through Sec translocon. PMID: 17233825 PMID: 7929011 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 16352602 PMID: 15838024 PMID: 7929011 PMID: 17233825Periplasmic, Peripheral outer membrane protein facing the periplasmG, F3Soluble IM FractionPeriplasm Periplasmic (Experimental)SECB, SRPSECnoyesN220.555SpI217.66433-00Yn7-16c21/22oPeriplasmic1031
ZUPT_ECOLIP0A8H3
zupT

ygiE

ygiEb3040 JW3008 integral IM protein (SEC in IM), Zinc transporter ZupT Integral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN590.198TMH010.378-67No6-25i32-50o62-81i125-150o170-192i199-215o235-256iCytoplasmicMembrane1027
YIHY_ECOLIP0A8K8
yihY
b3886 JW3857 b2268integral IM protein (SEC in IM), UPF0761 YihYIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane CytoplasmicSRPSECyesyesN600.127CYT0-0.200913-67No39-61i73-91o103-120i141-160o180-200i212-234o240-266iCytoplasmicMembrane10
FRDC_ECOLIP0A8Q0
frdC
b4152 JW4113 integral IM protein (SEC in IM), Fumarate reductase subunit C FrdCIntegral Inner Membrane Alpha-helcal transmembrane protein (experimental). Transmembrane helices: 3. N-terminal region is in cytoplasm. Helix A and B connected by very short turns to an interfacial helix (N51-L63)PMID: 24057063.PMID: 11266632 PMID: 10373108 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN590.224TMH01.78374-33Ni26-48o68-88i109-130oCytoplasmicMembrane109
FRDD_ECOLIP0A8Q3
frdD
b4151 JW4112 integral IM protein (SEC in IM), Fumarate reductase subunit D FrdDIntegral Inner Membrane Alpha-helcal transmembrane protein (experimental). Transmembrane helices: 3 PMID: 10373108.PMID: 11266632 PMID: 10373108 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN520.162TMH015.5267-33Ni12-45o57-78i99-118oCytoplasmicMembrane107
PSPC_ECOLIP0AFN2
pspC
b1306 JW1299 integral IM protein (SEC in IM), Phage shock C PspCIntegral Inner Membrane Defects in yidC and sec secretion induce psp operon. PMID: 14594846PMID: 14594846Integral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECProphage TransposaseyesnoN250.142CYT0-0.200913-11No39-64iCytoplasmicMembrane929
YMIA_ECOLIP0CB62
ymiA
b4522 JW1267.1 integral IM protein (SEC in IM), YmiAIntegral Inner Membrane Small membrane protein detected experimentallyPMID: 19121005Integral Inner MembraneBBinoutin/outN/AMembrane -SECnoyesN350.102TMH01.19842-11Ni20-41oCytoplasmicMembrane9
PHNE1_ECOLIP0DP69
phnE1
b4104 JW4065integral IM protein (SEC in IM), Putative cryptic phosphonate transport system permease PhnE1 Integral Inner Membrane Integral Inner MembraneBN/ACell inner membrane -SECpseudogene (Uniprot)N0.119CYT00.401335-33Yi30-50o92-116i137-157oUnknown
YCJF_ECOLIP0A8R7
ycjF
b1322 JW1315 integral IM protein (SEC in IM), UPF0283 YcjFIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.1CYT0-0.200913-34Ni70-87o99-120i214-236o256-275iCytoplasmicMembrane1010
YCEI_ECOLIP0A8X2
yceI
b1056 JW1043 periplasmic protein (SEC through IM), YceIPeriplasmicPMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY230.827SpI2223.1728-00Yn4-15c22/23oUnknown269
BLC_ECOLIP0A901
blc

yjeL

yjeLb4149 JW4110 OM lipoprotein (SEC through IM, inserted in OM by LOL), BlcOuter Membrane LipoproteinProven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130PMID: 15174130 PMID: 20932056 PMID: 21541338Outer Membrane LipoproteinIN/ACell outer membrane; Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY190.216SpII1814.624Pos+2=S00Yn3-15c20/21oOuterMembrane1052
BAMC_ECOLIP0A903
bamC

dapX nlpB

dapX nlpBb2477 JW2462 OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamCOuter Membrane LipoproteinOuter membrane protein related to resistance to ampicillin and tetracycline. Part of bam complex. Forms subcomplex with BamCE. Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384PMID: 16372265 PMID: 21823654 PMID: 21139201 PMID: 21370981 PMID: 15174130 PMID: 12488465 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 16079137 PMID: 20932056 PMID: 21541338Outer Membrane LipoproteinISoluble IM FractionCell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN320.177SpII2417.2458Pos+2=S00Yn13-23c27/28oOuterMembrane1086
LDRA_ECOLIP0DPD0
ldrA
b4419 JW5957integral IM protein (SEC in IM), Small toxic polypeptide LdrAIntegral Inner Membrane Potentially toxic protein.PMID: 19121005Integral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane CytoplasmicSRPSECyesyesN210.125CYT0-0.200913-11No6-31iCytoplasmicMembrane9
LDRC_ECOLIP0DPD1
ldrC
b4423 JW5959integral IM protein (SEC in IM), Small toxic polypeptide LdrCIntegral Inner Membrane Integral Inner MembraneBN/ACell inner membrane -SECN0.125CYT0-0.200913-11Yo6-31iUnknown
YIAB_ECOLIP11286
yiaB
b3563 JW5654 integral IM protein (SEC in IM), YiaBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN500.132TMH07.23112-44Ni7-26o32-50i62-87o93-110iCytoplasmicMembrane1038
YFIL_ECOLIP11289
yfiL
b2602 JW5412 OM lipoprotein (SEC through IM, inserted in OM by LOL), YfiLOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLyesyesY260.211SpII1612.903Pos+2=Q00Yn5-14c19/20oUnknown259
SLYB_ECOLIP0A905
slyB
b1641 JW1633 OM lipoprotein (SEC through IM, inserted in OM by LOL), SlyBOuter Membrane Lipoprotein Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384PMID: 15174130 PMID: 15911532 PMID: 10806384 PMID: 20932056 PMID: 21541338Outer Membrane LipoproteinISoluble IM FractionCell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN220.181SpII1719.9051Pos+2=V00Yn5-16c21/22oOuterMembrane9
MIPA_ECOLIP0A908
mipA

yeaF

yeaFb1782 JW1771 OM b-barrel protein (SEC through IM, inserted in OM by BAM), MltA-interacting protein MipAOuter Membrane b-barrel proteinMipA is considered to be a structural protein mediating the assembly of MltA to PBP1B into a complexPMID: 10037771. MipA couples both enzymes to one another in vivo, it is likely to give rise to a membrane adhesion site as depicted. Such contacts between the membranes. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000,Thein 2010)PMID: 15911532PMID: 10806384PMID: 12624733PMID: 20932056 PMID: 10037771PMID: 10037771 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 16079137 PMID: 20932056Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECBAMyesyesY230.835SpI2221.2923-00Yn6-17c22/23oOuterMembrane1011
OMPA_ECOLIP0A910
ompA

con tolG tut

con tolG tutb0957 JW0940 OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpAOuter Membrane b-barrel proteinSubstrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544PMID: 8626309 PMID: 17908933 PMID: 19343722 PMID: 19737520 PMID: 23641708 PMID: 12488465 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 9808047 PMID: 7813480 PMID: 16079137 PMID: 21778229 PMID: 20932056 PMID: 21541338 PMID: 19953543 PMID: 9298646 PMID: 24717922 PMID: 25664544Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)TFSECSKP,SURA,DEGP,DSBABAMyesyesY220.872SpI2128.1613-00Yn5-16c21/22oOuterMembrane1010
YJJA_ECOLIP18390
yjjA
b4360 JW5795 periplasmic protein (SEC through IM), YjjAPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY260.86SpI2523.6068-00Yn8-19c25/26oUnknown2100
FLIO_ECOLIP22586
fliO

flaP flbD

flaP flbDb1947 JW5316 Flagellar integral IM protein (SEC in IM), FliOIntegral Inner MembraneBBinoutout/inIM (predicted)Cell membrane Membrane anchoredflagellumSRPSECnoyesN270.161TMH00.0866025-11No18-39iCytoplasmicMembrane914
APPX_ECOLIP24244
appX

yccB

yccBb4592 JW0961.1 b0979.1integral IM protein (SEC in IM), Putative cytochrome bd-II ubiquinol oxidase subunit AppX Integral Inner Membrane Experimentally verified orientation: N(out)-C(in).PMID: 19121005 PMID: 21778229Integral Inner MembraneBBinoutout/inN/ACell inner membrane Integral Membrane ProteinSRPSECnoyesN210.107TMH02.07613-11No6-24iCytoplasmicMembrane9
PAL_ECOLIP0A912
pal

excC

excCb0741 JW0731 OM lipoprotein (SEC through IM), inserted in OM by LOL), PG-associated PalOuter Membrane Lipoprotein Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 10806384 PMID: 9298646PMID: 15174130 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 19364805 PMID: 23193105 PMID: 20932056 PMID: 9298646Outer Membrane LipoproteinISoluble IM FractionCell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY290.5SpII2121.6668Pos+2=S00Yn6-14c18/19oOuterMembrane1081
OMPW_ECOLIP0A915
ompW

yciD

yciDb1256 JW1248 OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpWOuter Membrane b-barrel proteinOuter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 9298646PMID: 16372265 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 16414958 PMID: 20932056 PMID: 9298646Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane ??-barrel protein (Experimental)SECBAMyesyesY220.754SpI2126.4914-00Yn4-15c21/22oOuterMembrane1042
OMPX_ECOLIP0A917
ompX

ybiG

ybiGb0814 JW0799 OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpXOuter Membrane b-barrel proteinSecB substrate. Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 9298646PMID: 16352602 PMID: 19343722 PMID: 22658749 PMID: 19737520 PMID: 12488465 PMID: 15911532 PMID: 10806384 PMID: 11266632 PMID: 10545325 PMID: 20932056 PMID: 21541338 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane; Multi-pass membrane proteinOuter Membrane ??-barrel protein (Experimental)SECB,TFSECSURA,SKPBAMyesyesY240.63SpI2326.0753-10Yn4-15c23/24oOuterMembrane1014
PA1_ECOLIP0A921
pldA
b3821 JW3794 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Phospholipase A1 PldAOuter Membrane b-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 PMID: 6397464PMID: 12624733 PMID: 11266632 PMID: 10537112 PMID: 6397463 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 6397464Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECSKPBAMyesyesY210.528SpI207.74532-00Yn3-14c20/21oOuterMembrane109
YIGF_ECOLIP27842
yigF
b3817 JW3790 integral IM protein (SEC in IM), YigFIntegral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECnoyesN400.108CYT0-0.200913-22Ni52-72o78-107iCytoplasmicMembrane925
YIGG_ECOLIP27843
yigG
b3818 JW5590 integral IM protein (SEC in IM), YigGIntegral Inner Membrane PMID: 11867724 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN420.121TMH05.14332-44Ni18-35o41-57i69-91o103-121iCytoplasmicMembrane10
PGPB_ECOLIP0A924
pgpB
b1278 JW1270 integral IM protein (SEC in IM), Phosphatidylglycerophosphatase B PgpBIntegral Inner Membrane PgpB protein has phosphatidyl glycerophosphate phosphatase activity higher in the cytoplasmic membrane, while it has phosphatidic acid and lysophosphatidic acid phosphatase activities higher in the outer membrane.PMID: 2846511 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN240.144TMH017.4928-66Ni12-29o49-68i75-96o157-178i185-206o212-230iCytoplasmicMembrane10
TSX_ECOLIP0A927
tsx

nupA

nupAb0411 JW0401 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Nucleoside-specific channel-forming Tsx Outer Membrane b-barrel proteinOuter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384PMID: 16372265 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 19953543Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)SECBAMProphage TransposaseyesnoY230.882SpI2224.8185-00Yn4-14c22/23oOuterMembrane100
WZA_ECOLIP0A930
wza
b2062 JW2047 OM lipoprotein & extra-cellular peripheral OM protein (SEC through IM), inserted in OM by LOL), Putative polysaccharide export Wza Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceHas a helical structure that crosses membrane. The transmembrane region of Wza is an alpha-helical barrel. Proven pignal peptidase II substrate. It has been demostrated that it can be also secreted and be surface exposed. PMID: 15174130PMCID: PMC3315050 PMID: 15174130 PMID: 10619844Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceI, F4N/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY260.13SpII2012.3938Pos+2=T00Yn11-21c25/26oOuterMembrane944
GFCE_ECOLIP0A932
gfcE

yccZ

yccZb0983 JW0966 OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative polysaccharide export GfcE Outer Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY230.202SpII2015.093Pos+2=T00Yn10-21c26/27oOuterMembrane959
YAAI_ECOLIP28696
yaaI
b0013 JW0012 periplasmic protein (SEC through IM), UPF0412 YaaIPeriplasmicGN/APeriplasmicSECyesyesY240.727SpI239.88216-00Yn4-15c23/24oUnknown259
YHHH_ECOLIP28911
yhhH
b3483 JW3449 integral IM protein (SEC in IM), YhhHIntegral Inner MembraneBBoutin/outIM (predicted)Membrane anchoredSRPSECnoyesN320.366CYT0-0.200913-11Ni12-30oUnknown252
YBFC_ECOLIP28915
ybfC
b0704 JW0693 periplasmic protein (SEC through IM), YbfCPrediction of three weak transmembrane helixes (one with 0.6 probability) in Phobius.PeriplasmicGN/APeriplasmicSECnoyesY200.787SpI193.48234-00Yn4-15c19/20oUnknown248
MLTA_ECOLIP0A935
mltA

mlt ygdM

mlt ygdMb2813 JW2784 OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase A MltAOuter Membrane LipoproteinThe formation of a complex between a murein polymerase (PBP1B) and a murein hydrolase (MltA). In the presence of MipA represents a first step in a reconstitution of the hypothetical murein-synthesizing holoenzyme. Postulated to be responsible for controlled growth of the stress-bearing sacculus of <i>E.coli</i>. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) PMID: 15174130 SRP recognizes the first signal anchor sequence of a polytopic membrane protein (MtlA) resulting in cotranslational targeting of MtlA to SecY and phospholipids of the plasma membrane. PMID: 10619852 Possible interaction of SRP with the secretory protein pOmpA is prevented by the association of trigger factor with nascent pOmpA. PMID: 10619852PMID: 15174130 PMID: 10037771 PMID: 12624733 PMID: 20932056 PMID: 10619852Outer Membrane LipoproteinIN/ACell outer membrane; Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLpseudogene (Ochman & Davalos, 2006)noyesY200.191SpII2022.7365Pos+2=S00Yn9-19c23/24oOuterMembrane1035
BAME_ECOLIP0A937
bamE

smpA

smpAb2617 JW2598 OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamEOuter Membrane LipoproteinPart of BAM complex. Forms subcomplex with BamCE.PMID: 21823654 PMID: 21139201 PMID: 21370981 PMID: 17404237 PMID: 20932056Outer Membrane LipoproteinISoluble IM FractionCell outer membrane Outer membrane LipoproteinSECLOLyesyesY240.197SpII1919.5033Pos+2=S00Yn5-16c20/21oOuterMembrane944
BAMA_ECOLIP0A940
bamA

yaeT yzzN yzzY

yaeT yzzN yzzYb0177 JW0172 OM b-barrel protein (SEC through IM, inserted in OM by BAM), assembly factor BamAOuter Membrane b-barrel proteinPart of bam complexPMID: 21823654PMID: 21370981. Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamAPMID: 23641708. Outer membrane protein determined by 2D gel electrophoresis (PMID: 15911532,PMID: 12624733,PMID: 20932056) Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 21823654 PMID: 21370981 PMID: 19525348 PMID: 23641708 PMID: 12624733 PMID: 10806384 PMID: 16079137 PMID: 20932056 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane Outer Membrane ss-barrel protein(experimental)SECBAMyesyesY210.641SpI2010.5737-00Yn6-13c20/21oOuterMembrane1032
MARB_ECOLIP31121
marB
b1532 JW1525 periplasmic protein (SEC through IM), Multiple antibiotic resistance MarB PeriplasmicGN/APeriplasmicSECnoyesY220.602SpI2118.3001-00Yn4-15c21/22oUnknown260
YDEI_ECOLIP31130
ydeI
b1536 JW1529 periplasmic protein (SEC through IM), YdeIPeriplasmicGN/APeriplasmicSECnoyesY200.882SpI1913.7979-00Yn3-14c19/20oUnknown2100
YIDI_ECOLIP31446
yidI
b3677 JW3653 integral IM protein (SEC in IM), YidIIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN360.149TMH00.964289-33Ni12-30o78-98i110-143oCytoplasmicMembrane1027
PLAP_ECOLIP0AA47
plaP

yeeF

yeeFb2014 JW5330 integral IM protein (SEC in IM), Low-affinity putrescine importer PlaPIntegral Inner Membrane Old gene name YeeF.PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.148TMH08.96423-1212Ni20-42o54-73i94-116o128-146i158-179o199-217i237-260o287-315i336-355o361-383i395-413o419-437iCytoplasmicMembrane10
YFDV_ECOLIP0AA49
yfdV
b2372 JW2369 integral IM protein (SEC in IM), transporter YfdVIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane Protein (Experimental)SRPSECnoyesN570.126CYT0-0.200913-1010No6-23i35-55o67-90i97-117o129-150i175-196o202-222i234-253o259-278i290-309oCytoplasmicMembrane10
QMCA_ECOLIP0AA53
qmcA

ybbK

ybbKb0489 JW0478 integral IM protein (SEC in IM), QmcAIntegral Inner Membrane PMID: 16573693Integral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN180.11TMH01.35378-10Yn2-13c18/19oUnknown213
YGHB_ECOLIP0AA60
yghB
b3009 JW2976 integral IM protein (SEC in IM) of DedA family, YghBIntegral Inner Membrane Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF.PMID: 15919996 PMID: 23086209Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.338TMH00.0786812-46No20-38i45-62o68-88i123-143o155-176i188-212oCytoplasmicMembrane108
YQJA_ECOLIP0AA63
yqjA
b3095 JW3066 integral IM protein (SEC in IM) of DedA family, YqjAIntegral Inner Membrane Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF.PMID: 15919996 PMID: 23086209Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein SRPSECyesyesN210.127TMH04.47784-66No20-38i45-62o68-88i123-143o155-176i188-212oCytoplasmicMembrane103
CBRB_ECOLIP31468
cbrB

yieI

yieIb3716 JW3694 integral IM protein (SEC in IM), CbrBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN340.114TMH014.4652-44Ni12-32o38-69i81-100o120-138iCytoplasmicMembrane1025
YDCD_ECOLIP31991
ydcD
b1457 JW1452 integral IM protein (SEC in IM), YdcDIntegral Inner MembraneBBoutin/outIM (predicted)Membrane anchoredSRPSECnoyesN320.142CYT0-0.200913-11Ni7-25oUnknown29
YIBG_ECOLIP32106
yibG
b3596 JW3570 periplasmic protein (SEC through IM), YibGPeriplasmicGN/APeriplasmicSECnoyesY200.567SpI190.904394-00Yn3-14c19/20oUnknown218
YIBI_ECOLIP32108
yibI
b3598 JW3572 integral IM protein (SEC in IM), YibIIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN270.12TMH012.0249-22No6-25i46-66oCytoplasmicMembrane1033
RHTA_ECOLIP0AA67
rhtA

ybiF

ybiFb0813 JW0798 integral IM protein (SEC in IM), Threonine/homoserine exporter RhtA Integral Inner Membrane PMID: 12648727 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN320.17TMH06.46418-109Yn10-18c22/23o38-59i71-89o95-115i122-139o145-165i177-196o202-225i237-258o264-283iCytoplasmicMembrane10
YEDA_ECOLIP0AA70
yedA
b1959 JW1942 integral IM protein (SEC in IM), transporter YedAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.125TMH011.0617-109Yn6-16c21/22o37-56i68-86o98-117i124-143o149-169i176-199o211-233i245-262o268-290iCytoplasmicMembrane10
YHBE_ECOLIP0AA73
yhbE
b3184 JW3151 integral IM protein (SEC in IM), transporter YhbEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.149TMH00.144382-1010Ni7-26o38-56i71-91o103-120i132-151o157-177i189-205o217-236i248-269o281-299iCytoplasmicMembrane10
DGOT_ECOLIP0AA76
dgoT

yidT

yidTb3691 JW5859 integral IM protein (SEC-YidC in IM), D-galactonate transporter DgoTIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN430.138TMH04.18214-1112Ni15-35o55-75i82-98o104-122i142-163o169-188i242-263o283-304i316-332o338-362i369-392o404-424iCytoplasmicMembrane10
EXUT_ECOLIP0AA78
exuT
b3093 JW3064 integral IM protein (SEC in IM), Hexuronate transporter ExuTIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN180.107TMH00.456812-1112Ni47-66o86-109i116-136o142-162i174-199o205-224i277-299o305-325i345-362o368-389i401-424o430-452iCytoplasmicMembrane10
YIIQ_ECOLIP32160
yiiQ
b3920 JW3891 periplasmic protein (SEC through IM), YiiQPeriplasmicGN/APeriplasmicSECyesyesY240.736SpI2314.7775-00Yn4-15c23/24oUnknown2
YIIX_ECOLIP32167
yiiX
b3937 JW3908 periplasmic protein (SEC through IM), YiiXPeriplasmicGN/APeriplasmicSECnoyesY190.818SpI1814.7441-00Yn5-13c18/19oUnknown227
YIJF_ECOLIP32668
yijF
b3944 JW3916 periplasmic protein (SEC through IM), YijFPeriplasmicGN/APeriplasmicSECyesyesY190.257SpI2011.5911-00Yn3-14c18/19oUnknown214
GARP_ECOLIP0AA80
garP

yhaU

yhaUb3127 JW3096 integral IM protein (SEC in IM), Probable galactarate transporter GarPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN330.195TMH03.99251-1112Ni12-32o52-73i85-103o109-130i151-173o179-199i251-270o290-314i326-345o351-372i379-406o412-434iCytoplasmicMembrane10
CODB_ECOLIP0AA82
codB
b0336 JW0327 integral IM protein (SEC-YidC in IM), Cytosine permease CodBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCnoyesN380.154TMH012.7134-1212Ni20-40o52-74i95-118o130-147i152-173o193-212i224-253o259-278i299-318o324-345i357-379o385-402iCytoplasmicMembrane10
DSBE_ECOLIP0AA86
dsbE

ccmG yejQ

ccmG yejQb2195 JW2183 integral IM protein (SEC in IM), Thiol:disulfide interchange DsbE Integral Inner Membrane PMID: 9537397 PMID: 16364305 Integral Inner MembraneBBoutin/outN/ACell inner membrane; Single-pass membrane protein; Periplasmic sidePeriplasmic with N-terminal membrane anchor (Experimental)SRPSECyesyesN290.264SpI278.59712-10Yn6-21c30/31oCytoplasmicMembrane1069
YEAY_ECOLIP0AA91
yeaY
b1806 JW1795 OM lipoprotein (SEC through IM, inserted in OM by LOL), YeaYOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN270.138SpII2214.0614Pos+2=V00Yn10-20c25/26oOuterMembrane925
YPDA_ECOLIP0AA93
ypdA
b2380 JW5388 integral IM protein (SEC in IM), Sensor histidine kinase YpdAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN160.103TMH01.16348-66No6-25i45-64o76-99i106-128o140-161i173-191oCytoplasmicMembrane10
YJBG_ECOLIP32688
yjbG
b4028 JW3988 periplasmic protein (SEC through IM), YjbGPeriplasmicGN/APeriplasmicSECyesyesY210.827SpI2017.0485-00Yn5-15c20/21oCytoplasmicMembrane9
PSPG_ECOLIP32696
pspG

yjbO

yjbOb4050 JW5716 integral IM protein (SEC in IM), Phage shock G PspGIntegral Inner Membrane PMID: 19555453Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECProphage TransposasenonoN320.133TMH016.1628-22Ni7-37o43-64iCytoplasmicMembrane9
YAFK_ECOLIP0AA99
yafK
b0224 JW0214 periplasmic protein (SEC through IM), Putative L,D-transpeptidase YafK PeriplasmicGN/APeriplasmicSECyesyesY200.74SpI1910.678-10Yn4-22c27/28oUnknown225
YAGU_ECOLIP0AAA1
yagU
b0287 JW0281 integral IM protein (SEC in IM), YagUIntegral Inner Membrane PMID: 16079137 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN300.316SpI293.31288-34Ni14-35o98-120i132-150o162-182iCytoplasmicMembrane1044
ECPA_ECOLIP0AAA3
ecpA

matB yagZ

matB yagZb0293 JW0287 Fimbrial periplasmic protein (SEC through IM), Common pilus major fimbrillin subunit EcpAPeriplasmicPutative chaperone???usher fimbrial operon yag (class ??); Fimbrial Subunit (yagZ). PMID: 16522795PMID: 20345943 PMID: 16522795PeriplasmicGN/AFimbrium Periplasmic (Experimental)fimbriaeSECnoyesY230.863SpI2225.6343-00Yn5-15c22/23oUnknown234
WZXE_ECOLIP0AAA7
wzxE

wzx yifJ

wzx yifJb3792 JW3766 integral IM protein (SEC in IM), Lipid III flippase WzxEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN410.139TMH05.34268-1211Yn6-16c21/22o45-67i79-105o117-138i145-164o176-195i216-237o257-278i299-322o334-355i362-382o394-412iCytoplasmicMembrane10
YBHL_ECOLIP0AAC4
ybhL
b0786 JW0769 integral IM protein (SEC in IM), YbhLIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.151TMH05.60898-77Ni20-42o57-77i89-107o113-131i143-161o167-186i207-230oCytoplasmicMembrane10
YJCB_ECOLIP32700
yjcB
b4060 JW5718 integral IM protein (SEC in IM), YjcBIntegral Inner Membrane PMID: 19555453Integral Inner MembraneBBpolyout/outIM (predicted)Integral Membrane ProteinSRPSECyesyesN650.12TMH09.92398-22No12-29i36-56oCytoplasmicMembrane945
NRFF_ECOLIP32711
nrfF

yjcM

yjcMb4075 JW4036 integral IM protein (SEC in IM), Formate-dependent nitrite reductase complex subunit NrfF Integral Inner MembraneBBoutout/inIM (predicted)Periplasm Membrane anchoredSRPSECnoyesY190.845SpI1816.61-11Yn4-14c18/19o103-121iCytoplasmicMembrane1020
MOKC_ECOLIP33236
mokC

gefL

gefLb0018 JW5879 integral IM protein (SEC in IM), Regulatory MokCIntegral Inner MembraneBBoutin/outN/ACell inner membrane PeriplasmicSRPSECnoyesN410.287SpI401.37208-11Ni25-44oPeriplasmic9
YEHE_ECOLIP33344
yehE
b2112 JW2099 periplasmic protein (SEC through IM), YehEPeriplasmicYeh operon is homolog to fim operon (yehd is fimI homolog).PMID: 20345943PeriplasmicGN/APeriplasmicSECnoyesY230.529SpI2211.8793-00Yn6-17c22/23oCytoplasmicMembrane949
YCCA_ECOLIP0AAC6
yccA
b0970 JW0953 integral IM protein (SEC in IM), Modulator of FtsH protease YccA Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane protein Integral Membrane ProteinSRPSECyesyesN420.268TMH07.78202-77Ni20-41o47-67i74-94o106-126i133-154o160-184i196-216oCytoplasmicMembrane10
MTR_ECOLIP0AAD2
mtr
b3161 JW3130 integral IM protein (SEC in IM), Tryptophan-specific transport MtrIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN470.192TMH019.2156-1111Ni12-34o40-65i86-107o127-145i152-170o190-207i228-246o284-309i321-340o346-367i388-410oCytoplasmicMembrane10
TYRP_ECOLIP0AAD4
tyrP
b1907 JW1895 integral IM protein (SEC in IM), Tyrosine-specific transport TyrPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN290.164TMH011.6392-1110Yn6-17c29/30o39-58i78-100o120-141i148-169o181-204i216-235o273-298i310-330o336-355i376-399oCytoplasmicMembrane10
SDAC_ECOLIP0AAD6
sdaC

dcrA

dcrAb2796 JW2767 integral IM protein (SEC in IM), Serine transporter SdaCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN360.132TMH010.9503-1111Ni24-43o49-67i100-120o140-157i164-187o207-229i250-273o300-324i345-364o370-394i406-428oCytoplasmicMembrane10
TDCC_ECOLIP0AAD8
tdcC
b3116 JW3087 integral IM protein (SEC in IM), Threonine/serine transporter TdcC Integral Inner Membrane Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 15919996 PMID: 25403562Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECYfgMyesyesN350.131TMH09.58087-1111Ni23-42o48-66i97-116o136-155i167-186o206-234i255-278o317-337i358-379o391-409i421-440oCytoplasmicMembrane10
PPDA_ECOLIP33554
ppdA
b2826 JW2794 periplasmic protein (SEC through IM), Prepilin peptidase-dependent A PpdAPeriplasmicGN/APeriplasmicSECyesyesN270.172TMH07.10154-11Ni12-33oCytoplasmicMembrane910
YBBC_ECOLIP33668
ybbC
b0498 JW0487 OM lipoprotein (SEC through IM, inserted in OM by LOL), YbbCOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesY180.367SpII178.97285Pos+2=N00Yn6-17c25/26oUnknown248
CYCA_ECOLIP0AAE0
cycA

dagA ytfD

dagA ytfDb4208 JW4166 integral IM protein (SEC-YidC in IM), D-serine/D-alanine/glycine transporter CycAIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN530.108TMH03.35425-1212Ni28-45o51-71i108-130o136-154i166-187o207-231i252-274o286-311i347-365o371-397i417-435o441-460iCytoplasmicMembrane10
PROY_ECOLIP0AAE2
proY

yajM

yajMb0402 JW5055 integral IM protein (SEC in IM), Proline-specific permease ProY Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN480.136TMH06.32309-1212Ni16-34o40-59i95-118o124-142i154-176o196-218i239-258o278-298i330-350o356-377i398-421o427-445iCytoplasmicMembrane10
ARCD_ECOLIP0AAE5
ydgI
b1605 JW1597 nucleoid & integral IM protein (SEC in IM), Putative arginine/ornithine antiporter YdgIIntegral Inner Membrane, NucleoidPMID: 23138451 PMID: 15919996Integral Inner Membrane, NucleoidB, Nout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.206TMH08.33129-1312Yn5-16c25/26o41-61i82-107o127-145i157-178o204-222i234-257o283-311i332-352o358-378i385-402o408-426i441-459oCytoplasmicMembrane10
CADB_ECOLIP0AAE8
cadB
b4132 JW4093 integral IM protein (SEC-YidC in IM), Probable cadaverine/lysine antiporter CadBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN180.152TMH09.00829-1212Ni7-28o40-58i94-115o121-139i146-169o189-208i220-238o258-283i323-344o350-371i383-401o407-424iCytoplasmicMembrane10
YBBD_ECOLIP33669
ybbD
b0501 JW0489 integral IM protein (SEC in IM), Putative YbbDIntegral Inner MembraneBBinout/inIM (predicted)Membrane Membrane anchoredSRPSECpseudogene (Uniprot) nonoN350.114TMH09.05171-11No12-32iCytoplasmicMembrane946
ASR_ECOLIP36560
asr
b1597 JW5826 periplasmic protein (SEC through IM), Acid shock protein AsrPeriplasmicPMID: 12670971PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY220.907SpI2128.9272-00Yn4-15c21/22oPeriplasmic10
PPDD_ECOLIP36647
ppdD
b0108 JW0104 integral IM protein (SEC in IM), Prepilin peptidase-dependent D PpdDIntegral Inner MembraneBBoutin/outIM (predicted)FimbriumMembrane anchoredSRPSECnoyesN530.143TMH04.07217-11Ni12-30oExtracellular99
HOKA_ECOLIP37305
hokA

yiaZ

yiaZb4455 JW3526 integral IM protein (SEC in IM), HokAIntegral Inner Membrane Potentially toxic protein.PMID: 19121005Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchoredSRPSECnoyesN250.11CYT0-0.200913-11Ni7-24oPeriplasmic9110
POTE_ECOLIP0AAF1
potE
b0692 JW0679 integral IM protein (SEC in IM), Putrescine transporter PotE PotEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN500.144TMH05.94665-1212Ni12-30o42-60i90-110o122-140i152-172o184-204i225-249o276-300i321-345o351-374i386-404o410-428iCytoplasmicMembrane10
MDLB_ECOLIP0AAG5
mdlB

mdl

mdlb0449 JW5061 integral IM protein (SEC in IM), Multidrug resistance-like ATP-binding MdlB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN380.136SpI374.21577-44Yn25-32c37/38o61-81i138-161o167-185i250-273oCytoplasmicMembrane106
FTSH_ECOLIP0AAI3
ftsH

hflB mrsC std tolZ

hflB mrsC std tolZb3178 JW3145 integral IM protein (SEC in IM), ATP-dependent zinc metalloprotease FtsHIntegral Inner Membrane Trigger factor-sensitive aggregation. PMID: 8444797 PMID: 1925026 PMID: 8106505 PMID: 8106505 PMID: 7781608PMID: 7500942 PMID: 19737520 PMID: 8444797 PMID: 8947034 PMID: 15919657 PMID: 15919996 PMID: 1925026 PMID: 8106505 PMID: 7781608Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRP,TFSECnoyesN240.152SpI212.2847-21Yn5-16c21/22o98-119iCytoplasmicMembrane1041
YNFG_ECOLIP0AAJ1
ynfG
b1589 JW1581 periplasmic peripheral IM protein (TAT via piggyback through IM), Probable anaerobic dimethyl sulfoxide reductase chain YnfG Peripheral inner membrane protein facing the periplasmThe physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown. DmsB paralog. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. ynfFGH are expressed anaerobically. YnfG can function with DmsAC, replacing DmsB a as the electron transfer subunit. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/APeriplasmicTAT (piggyback)yesyesN440.106CYT0-0.200913-00NiUnknown538
YBCH_ECOLIP37325
ybcH
b0567 JW0556 periplasmic protein (SEC through IM), YbcHPeriplasmicGN/APeriplasmicSECnoyesY210.6SpI2014.6445-10Yn4-15c20/21oCytoplasmicMembrane926
YHHL_ECOLIP37614
yhhL
b3466 JW5683 integral IM protein (SEC in IM), YhhLIntegral Inner MembraneBBinoutout/outIM (predicted)Integral Membrane ProteinSRPSECnoyesN250.114TMH01.98334-21Yn8-19c24/25o34-51iCytoplasmicMembrane947
FDNH_ECOLIP0AAJ3
fdnH
b1475 JW1471 integral IM protein (SEC in IM), Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit FdnHIntegral Inner Membrane Part of the formate dehydrogenase N (FDH-N) of Escherichia coli which is a membrane-bound enzyme comprising FdnG, FdnH, and FdnI subunits. PMID: 11884747 PMID: 16858726PMID: 15342602 PMID: 16858726 PMID: 11266632 PMID: 11884747Integral Inner MembraneBBinin/outIM (predicted)Cell inner membrane Membrane anchored (Experimental)SRPSECyesyesN240.116CYT0-0.200913-11Ni260-279oCytoplasmicMembrane1080
HYBA_ECOLIP0AAJ8
hybA
b2996 JW2964 periplasmic peripheral IM protein (TAT-SEC through IM), Hydrogenase-2 operon HybAPeripheral inner membrane protein facing the periplasmTAT secretion system. Part of membrane-associated hydrogenase 4 complex. PMID: 9738917PMID: 15911532 PMID: 22313414 PMID: 21890895 PMID: 9738917Peripheral inner membrane protein facing the periplasmF2IM (predicted)PeriplasmPeriplasmic (Experimental)TAT,SECyesyesY270.442SpI2619.7463-01Yn9-20c26/27o293-311iCytoplasmicMembrane939
NRFC_ECOLIP0AAK7
nrfC

yjcJ

yjcJb4072 JW4033 periplasmic protein (TAT through IM), NrfCPeriplasmicTAT secretion system. PMID: 15280383PMID: 17218314 PMID: 15280383PeriplasmicGN/APeriplasmic (Experimental)TATyesyesN280.447SpI277.45987-00Yn8-19c27/28oCytoplasmic1023
NAPG_ECOLIP0AAL3
napG

yojA yojB

yojA yojBb2205 JW2193 periplasmic protein (TAT through IM), Ferredoxin-type NapGPeriplasmicTAT secretion system. PMID: 11967083PMID: 17218314 PMID: 11967083PeriplasmicGN/APeriplasm Periplasmic (Experimental)TATyesyesN420.316SpI410.19393-00Yn21-32c41/42oCytoplasmic820
CYBH_ECOLIP0AAM1
hyaC
b0974 JW0956 integral IM protein (SEC in IM), Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit HyaCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.112TMH010.0819-46No20-40i61-80o100-116i128-151o157-174i186-203oCytoplasmicMembrane10
YHHM_ECOLIP37615
yhhM
b3467 JW3432 integral IM protein (SEC in IM), YhhMSingle transmembrane helice predicted which would be removed upon signal peptide cleavage IM-cter inside.Integral Inner MembraneBBinout/inN/AMembrane anchoredSRPSECyesyesN320.135CYT0-0.200913-11No6-24iUnknown275
SIEB_ECOLIP38392
sieB
b1353 JW5209 integral IM protein (SEC in IM), Superinfection exclusion B SieBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN420.105TMH03.43476-22Ni20-38o50-70iCytoplasmicMembrane1013
DGCC_ECOLIP0AAP1
dgcC

adrA yaiC

adrA yaiCb0385 JW0376 integral IM protein (SEC in IM), Probable diguanylate cyclase DgcCIntegral Inner Membrane PMID: 10760159 PMID: 12673057 PMID: 16585767Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane Integral Membrane Protein(experimental)SRPSECyesyesN200.116CYT0-0.200913-55Ni43-61o67-85i106-130o142-167i174-193oCytoplasmicMembrane1022
YBBJ_ECOLIP0AAS3
ybbJ
b0488 JW5065 integral IM protein (SEC in IM), YbbJIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.17TMH018.8226-33No6-23i30-49o55-72iCytoplasmicMembrane1012
YBDG_ECOLIP0AAT4
ybdG

mscM

mscMb0577 JW0566 integral IM protein (SEC in IM), Miniconductance mechanosensitive channel YbdG Integral Inner Membrane Mechanosensitive channel of miniconductance activity (MscM).PMID: 20616037 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN390.102CYT0-0.200913-55No23-48i68-87o107-127i139-160o166-183iCytoplasmicMembrane1011
ACRZ_ECOLIP0AAW9
acrZ

ybhT

ybhTb0762 JW5102 integral IM protein (SEC-YidC in IM), Multidrug efflux pump accessory AcrZ Integral Inner Membrane Experimentally verified orientation: N(out)-C(in). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchoredSRPSEC-YidCnoyesN210.157TMH01.49031-11Ni7-29oCytoplasmicMembrane928
YBIS_ECOLIP0AAX8
ybiS
b0819 JW0803 periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase YbiSPeripheral outer membrane protein facing the periplasmPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. YbiS has been shown to crosslink Braun's lipoprotein (Lpp) to the peptidoglycan. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 18498364 PMID: 23832002 PMID: 9298646 PMID: 22030350Peripheral outer membrane protein facing the periplasmF3N/APeriplasmPeriplasmic (Experimental)SECSURAyesyesY250.584SpI2419.8413-10Yn8-19c24/25oUnknown240
YTFJ_ECOLIP39187
ytfJ
b4216 JW4175 periplasmic protein (SEC through IM), YtfJPeriplasmicPMID: 9298646PeriplasmicGN/APeriplasm Periplasmic(experimental)SECyesyesY210.734SpI2013.6826-00Yn6-14c22/23oUnknown218
YJEO_ECOLIP39284
yjeO
b4158 JW4119 integral IM protein (SEC in IM), YjeOIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN260.227TMH07.24951-32Yn6-17c25/26o55-72i79-98oCytoplasmicMembrane1056
ELYC_ECOLIP0AB01
elyC

ycbC

ycbCb0920 JW0903 integral IM protein (SEC in IM), Envelope biogenesis factor ElyCIntegral Inner Membrane Shown to play a critical role in the metabolism of the essential lipid carrier used for the biogenesis of cell wall and other bacterial surface polysaccharides.PMCID: PMC3879167Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN600.155TMH09.49418-22No12-32i41-59oCytoplasmicMembrane924
YCBK_ECOLIP0AB06
ycbK
b0926 JW0909 periplasmic protein (TAT through IM), YcbKPeriplasmicTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTATyesyesN310.559SpI308.29508-10Yn11-22c30/31oUnknown211
YMBA_ECOLIP0AB10
ymbA
b0952 JW5127 IM lipoprotein (SEC through IM), inserted in OM by LOL), Intertransport PqiC YmbAputative protase (enterobacterium homolog)Inner Membrane LipoproteinEN/ACell outer membrane CytoplasmicSECLOLyesyesN200.205SpII1522.8198Pos+2=S00Yn4-14c19/20oUnknown282
YCCF_ECOLIP0AB12
yccF
b0961 JW0944 integral IM protein (SEC in IM), YccFIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN240.116TMH02.25765-33Ni7-33o69-90i102-123oCytoplasmicMembrane10
EFEO_ECOLIP0AB24
efeO

ycdO

ycdOb1018 JW1003 periplasmic protein (SEC through IM), Iron uptake system component EfeO Periplasmic Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 16551627 PMID: 19953543 PMID: 12107143 PMID: 22030350 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECDSBAyesyesY270.767SpI2618.2235-00Yn9-18c26/27oPeriplasmic1042
YTFB_ECOLIP39310
ytfB
b4206 JW5745 integral IM protein (SEC in IM), Cell division YtfBIntegral Inner MembraneBBinoutin/outIM (predicted)Cell inner membrane CytoplasmicSRPSECnoyesN570.108CYT0-0.200913-01Ni30-48oExtracellular957
YJGX_ECOLIP39349
yjgX
b4575 b4275/b4276/b4560integral IM protein (SEC in IM), Putative phosphoethanolamine transferase YjgX Integral Inner MembraneBBinout/inIM (predicted)Cell inner membrane CytoplasmicSRPSECpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN310.126TMH04.71526-11No6-25iCytoplasmicMembrane1049
YJHE_ECOLIP39355
yjhE
b4282 integral IM protein (SEC in IM), Putative YjhEIntegral Inner MembraneB-IM (predicted)Cell membrane Integral Membrane ProteinSRPSECpseudogene (Uniprot) nonoN380.334CYT0-0.200913-22Ni22-49o55-76iCytoplasmicMembrane1015
YJIH_ECOLIP39379
yjiH
b4330 JW5783 integral IM protein (SEC in IM), YjiHIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN380.143TMH05.26808-44Ni21-39o86-104i116-135o176-201iCytoplasmicMembrane916
YCEB_ECOLIP0AB26
yceB
b1063 JW1050 OM lipoprotein (SEC through IM, inserted in OM by LOL), YceBOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinISoluble IM FractionCell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN550.134SpII1816.4921Pos+2=N00Yn4-15c19/20oUnknown245
LPOB_ECOLIP0AB38
lpoB

ycfM

ycfMb1105 JW5157 OM lipoprotein (SEC through IM), inserted in OM by LOL), Penicillin-binding activator LpoB Outer Membrane LipoproteinThe two OM Lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 21183073 PMID: 21183074 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY240.258SpII1923.7759Pos+2=V00Yn8-18c22/23oUnknown296
BHSA_ECOLIP0AB40
bhsA

comC ycfR

comC ycfRb1112 JW1098 periplasmic peripheral OM protein (SEC through IM), Multiple stress resistance BhsA Peripheral outer membrane protein facing the periplasmBy membrane fractionation, ComC was shown to be localized in the outer membrane.PMID: 22089859Peripheral outer membrane protein facing the periplasmF3N/ACell outer membrane PeriplasmicSECyesyesY230.773SpI2221.6297-00Yn6-14c22/23oUnknown270
LAPB_ECOLIP0AB58
LapB

yciM

yciMb1280 JW1272 integral IM protein (SEC in IM), polysaccharide assembly B LapBIntegral Inner Membrane Potential TAT secreted protein. Required to maintain cell envelope integrity. Heat shock protein that is involved in the assembly of LPS PMID: 24722986 Heat shock protein that is involved in the assembly of LPS PMID: 24722986 Heat shock protein involved in the assembly of LPS. In pull-down experiments, LapA and LapB co-purified with LPS, Lpt proteins, FtsH (protease), DnaK, and DnaJ (chaperones). A specific interaction was also observed between WaaC and LapB. LapB potentially coordinates assembly of proteins involved in LPS synthesis at the plasma membrane and regulates turnover of LpxC. PMID: 25403562PMID: 24187084 PMID: 25403562Integral Inner MembraneB-Soluble IM FractionCell inner membrane PeriplasmicSECyesyesN250.264CYT0-0.200913-00Yn4-11c16/17oCytoplasmic89
YGJQ_ECOLIP42598
ygjQ
b3086 JW3057 integral IM protein (SEC in IM), YgjQIntegral Inner MembraneBBoutin/outN/AMembrane PeriplasmicSRPSECnoyesN370.199SpI366.02153-10Yn21-31c36/37oCytoplasmicMembrane9
YGJV_ECOLIP42603
ygjV
b3090 JW3061 integral IM protein (SEC in IM), YgjVIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN560.156TMH04.26984-55No6-24i36-59o71-89i101-124o136-157iCytoplasmicMembrane10
MZRA_ECOLIP42615
mzrA

ecfM yqjB

ecfM yqjBb3096 JW3067 integral IM protein (SEC in IM), Modulator of the activity of the EnvZ/OmpR two-component regulatory system MzrAIntegral Inner Membrane PMID: 19432797 PMID: 20889743Integral Inner MembraneBBoutin/outN/ACell inner membrane Membrane anchoredSRPSECyesyesN300.154SpI294.521-10Yn11-22c27/28oUnknown214
YQJC_ECOLIP42616
yqjC
b3097 JW5516 periplasmic protein (SEC through IM), YqjCPeriplasmicGN/APeriplasmicSECyesyesY210.508SpI209.52426-00Yn5-15c20/21oUnknown279
PNTB_ECOLIP0AB67
pntB
b1602 JW1594 integral IM protein (SEC in IM), NAD(P) transhydrogenase subunit beta PntBIntegral Inner Membrane PMID: 14660605 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.125TMH09.6368-99No6-24i36-51o57-74i86-105o125-143i163-180o186-207i214-234o240-260iCytoplasmicMembrane10
APBE_ECOLIP0AB85
apbE

yojK yojL

yojK yojLb2214 JW5368 IM lipoprotein (SEC through IM), inserted in OM by LOL), FAD:FMN transferase ApbEInner Membrane LipoproteinProven signal peptidase II substrate.PMID: 15174130Inner Membrane LipoproteinEIM (predicted)Cell inner membrane Inner membrane lipoproteinSECLOLyesyesN280.154SpII1912.0176Pos+2=D00Yn8-18c22/23oUnknown265
ARSB_ECOLIP0AB93
arsB

arsF

arsFb3502 JW3469 integral IM protein (SEC in IM), Arsenical pump ArsBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN440.23TMH08.42262-1110Yn2-17c35/36o51-70i91-109o115-132i139-163o175-198i222-240o246-263i275-296o316-334i401-423oCytoplasmicMembrane10
ATP6_ECOLIP0AB98
atpB

papD uncB

papD uncBb3738 JW3716 integral IM protein (SEC-YidC in IM), ATP synthase subunit a AtpBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 10806384PMID: 22108384 PMID: 15263011 PMID: 15919996 PMID: 10806384Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSEC-YidCyesyesN670.123CYT0-0.200913-55No36-60i100-118o147-166i211-233o239-264iCytoplasmicMembrane107
YQJF_ECOLIP42619
yqjF
b3101 JW5850 integral IM protein (SEC in IM), YqjFIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.118TMH08.80873-44Ni7-26o46-66i73-91o103-122iCytoplasmicMembrane10
YGAH_ECOLIP43667
ygaH
b2683 JW2658 integral IM protein (SEC in IM), YgaHIntegral Inner MembraneBBpolyout/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN180.142CYT0-0.200913-33Yn5-15c20/21o36-57i69-85o91-109iCytoplasmicMembrane10
YHCE_ECOLIP45421
yhcE
b4569 b3217Fimbrial periplasmic protein (SEC through IM), Putative YhcEPeriplasmicPotential fimbrial yhc operon (class ??); Uknown function.PMID: 20345943PeriplasmicGN/APeriplasmicfimbriaeSECpseudogene (Uniprot)nonoY200.69SpI194.02903-00Yn4-11c16/17oUnknown220
YHCF_ECOLIP45422
yhcF
b3219 JW3188 Fimbrial periplasmic protein (SEC through IM), YhcFPeriplasmicPotential fimbrial yhc operon (class ??); Fimbrial Subunit.PMID: 20345943PeriplasmicGN/APeriplasmicfimbriaeSECnoyesY210.835SpI2016.471-00Yn6-16c20/21oUnknown27
ATPF_ECOLIP0ABA0
atpF

papF uncF

papF uncFb3736 JW3714 integral IM protein (SEC in IM), ATP synthase subunit b AtpFIntegral Inner Membrane PMID: 15263011 PMID: 21541338 PMID: 6278247Integral Inner MembraneBBinout/inSoluble IM FractionCell inner membrane Membrane anchored (Experimental)SRPSECnoyesN320.35CYT0-0.200913-11No6-26iCytoplasmicMembrane1019
ATMA_ECOLIP0ABB8
mgtA

corB mgt

corB mgtb4242 JW4201 integral IM protein (SEC in IM), Magnesium-transporting ATPase, P-type 1 MgtAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN470.122CYT0-0.200913-78Ni89-112o118-137i291-309o315-342i768-791o803-821i833-857o869-891iCytoplasmicMembrane1050
ATPZ_ECOLIP0ABC0
atpI

uncI

uncIb3739 JW5611 integral IM protein (SEC-YidC in IM), ATP synthase I AtpIIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN640.113TMH04.01303-43Yn14-25c30/31o40-59i71-94o100-122iCytoplasmicMembrane1034
HFLC_ECOLIP0ABC3
hflC

hflA

hflAb4175 JW4133 integral IM protein (SEC in IM), Modulator of FtsH protease HflC Integral Inner Membrane N-terminally located transmembrane segment. PMID: 9792691 Translocation of the C-terminal domains of HflKC was retarded markedly when sodium azide was added to inhibit SecA ATPase and blocked almost completely in secY- or secD-defective mutant cells. PMID: 9792691PMID: 8947034 PMID: 16834335 PMID: 9792691Integral Inner MembraneBBinout/inSoluble IM FractionCell inner membrane Membrane anchored (Experimental)SRPSECProphage Transposase (by homology to BL21)yesyesN250.135CYT0-0.200913-11No6-23iCytoplasmic846
YHDT_ECOLIP45566
yhdT
b3257 JW3225 integral IM protein (SEC in IM), YhdTIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN300.15TMH015.9222-22Ni12-33o45-65iCytoplasmicMembrane943
YBCI_ECOLIP45570
ybcI
b0527 JW0516 integral IM protein (SEC in IM), YbcIIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.121TMH04.35035-44Ni27-47o59-78i85-103o149-170iCytoplasmicMembrane104
HOFP_ECOLIP45750
hofP

yrfA

yrfAb3392 JW5694 periplasmic protein, DNA utilization HofPNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.PeriplasmicGIM (predicted-detected)CytoplasmicnoyesN210.138CYT0-0.200913-00Yn6-16c20/21oUnknown2
HFLK_ECOLIP0ABC7
hflK

hflA

hflAb4174 JW4132 integral IM protein (SEC in IM), Modulator of FtsH protease HflK Integral Inner Membrane Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 N-terminally located transmembrane segment. PMID: 9792691 Translocation of the C-terminal domains of HflKC was retarded markedly when sodium azide was added to inhibit SecA ATPase and blocked almost completely in secY- or secD-defective mutant cells. PMID: 9792691PMID: 8947034 PMID: 21541338 PMID: 9792691Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchoredSRPSECProphage TransposaseyesnoN30.101CYT0-0.200913-11Ni80-100oUnknown520
BETT_ECOLIP0ABC9
betT
b0314 JW0306 integral IM protein (SEC in IM), High-affinity choline transport protein BetTIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN370.108TMH011.6116-1212Ni16-35o55-79i91-115o143-166i194-215o235-253i265-290o319-338i350-368o403-422i448-467o479-497iCytoplasmicMembrane10
YEAV_ECOLIP0ABD1
yeaV
b1801 JW5293 integral IM protein (SEC in IM), transporter YeaVIntegral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN190.119TMH02.33182-1011Yn2-13c18/19o34-59i80-104o136-158i170-191o203-221i228-246o258-277i289-308o348-371i392-412o418-438iCytoplasmicMembrane10
C561_ECOLIP0ABE5
cybB
b1418 JW5224 integral IM protein (SEC-YidC in IM), Cytochrome b561 CybBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCnoyesN230.142TMH07.83021-44Ni12-33o39-62i82-106o143-162iCytoplasmicMembrane1031
HOFO_ECOLIP45751
hofO

yrfB

yrfBb3393 JW3356 integral IM protein (SEC in IM), DNA utilization HofOIntegral Inner MembraneBBinout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECyesyesN400.213TMH06.9432-11No20-38iUnknown219
YBJC_ECOLIP46119
ybjC
b0850 JW0834 integral IM protein (SEC in IM), YbjCIntegral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN260.131TMH06.40394-22Ni12-29o44-68iCytoplasmicMembrane945
YAJI_ECOLIP46122
yajI
b0412 JW5056 OM lipoprotein (SEC through IM, inserted in OM by LOL), YajIOuter Membrane LipoproteinIN/ACell membrane Outer membrane LipoproteinSECLOLnoyesY240.215SpII1918.9902Pos+2=V00Yn8-18c23/24oUnknown288
YFIM_ECOLIP46126
yfiM
b2586 JW2570 IM lipoprotein (SEC through IM, inserted in OM by LOL), YfiMContains the following signature IPR018736 Protein of unknown function DUF2279,Periplasmic lipoproteinInner Membrane LipoproteinEN/ACytoplasmicSECLOLyesyesY200.599SpII1415.2395Pos+2=S00Yn3-13c18/19oCytoplasmicMembrane912
PGSA_ECOLIP0ABF8
pgsA
b1912 JW1897 integral IM protein (SEC in IM), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsAIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 12558186 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN350.17TMH012.5336-46Ni7-27o33-52i64-83o89-108i120-142o148-169iCytoplasmicMembrane107
CDSA_ECOLIP0ABG1
cdsA

cds

cdsb0175 JW5810 integral IM protein (SEC in IM), Phosphatidate cytidylyltransferase CdsAIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. PMID: 15919996PMID: 9008159 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRP (exp)SECyesyesN300.287TMH018.3357-88Yn6-13c18/19o28-44i56-73o93-113i120-141o153-171i192-210o216-234i264-282oCytoplasmicMembrane10
FTSW_ECOLIP0ABG4
ftsW
b0089 JW0087 integral IM protein (SEC in IM), Probable PG glycosyltransferase FtsW Integral Inner Membrane Interacts directly with FtsN. The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex.PMID: 20847002 PMID: 20497333 PMID: 9603865 PMID: 12423747 PMID: 11807049 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN670.137TMH010.7628-109Yn15-26c32/33o48-66i87-104o110-129i175-192o198-215i222-239o307-328i340-364o376-395iCytoplasmicMembrane10
RODA_ECOLIP0ABG7
mrdB

rodA

rodAb0634 JW0629 integral IM protein (SEC in IM), PG glycosyltransferase MrdB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN410.198TMH06.46483-99No20-38i50-68o74-95i136-154o160-176i183-202o263-289i310-332o338-359iCytoplasmicMembrane10
YGBE_ECOLIP46141
ygbE
b2749 JW2719 integral IM protein (SEC in IM), YgbEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.233TMH010.6872-33No23-46i53-79o85-106iCytoplasmicMembrane10
YGGM_ECOLIP46142
yggM
b2956 JW2923 periplasmic protein (SEC through IM), YggMPeriplasmicGN/APeriplasmicSECyesyesY220.874SpI2116.8631-00Yn5-16c21/22oPeriplasmic932
RSEC_ECOLIP46187
rseC
b2570 JW2554 integral IM protein (SEC in IM), RseCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN250.187CYT0-0.200913-22Ni79-98o104-121iCytoplasmicMembrane1027
CORA_ECOLIP0ABI4
corA
b3816 JW3789 integral IM protein (SEC-YidC in IM), Magnesium transport CorA Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. Single particle electron microscopy analysis and two-dimensional crystals of PPD (large soluble periplasmic domain) of CorA indicated that E. coli CorA PPD is a pyramid-like homotetramer with a central cavity.PMID: 22108384 PMID: 15919996 PMID: 16835234Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN270.101CYT0-0.200913-22Ni255-278o290-310iCytoplasmicMembrane1065
CYOB_ECOLIP0ABI8
cyoB
b0431 JW0421 integral IM protein (SEC-YidC in IM), Cytochrome bo(3) ubiquinol oxidase subunit 1 CyoBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 11017202 PMID: 11266632 PMID: 22108384 PMID: 6365921 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN310.108TMH010.2812-1414No16-38i59-79o103-126i146-170o190-213i233-255o275-298i310-334o346-368i380-403o415-436i457-479o491-518i593-626oCytoplasmicMembrane10
CYOA_ECOLIP0ABJ1
cyoA
b0432 JW0422 integral IM protein (SEC in IM), Cytochrome bo(3) ubiquinol oxidase subunit 2 CyoAIntegral Inner MembraneProven signal peptidase II substrate PMID: 15174130. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 The SRP interacts with the first TM of CyoA (rather than with its signal sequence) to mediate targeting of CyoA to the Sec/YidC insertion site in the inner membrane. PMID: 16481320 Efficient insertion of in vitro synthesized pre-CyoA into proteoliposomes requires YidC, SecYEG, and SecA and occurs independently of the proton motive force. PMID: 16513637PMID: 15174130 PMID: 11266632 PMID: 11017202 PMID: 6365921 PMID: 16079137 PMID: 15919657 PMID: 15919996 PMID: 21541338 PMID: 16481320 PMID: 16513637Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN310.189TMH011.3494-32Yn10-22c27/28o43-67i88-108oCytoplasmicMembrane1019
AAEX_ECOLIP46478
aaeX

yhcR

yhcRb3242 JW5541 integral IM protein (SEC in IM), AaeXIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN210.106TMH010.7464-22No6-31i43-62oCytoplasmicMembrane934
YQGD_ECOLIP46879
yqgD
b2941 JW2908 integral IM protein (SEC in IM), YqgDIntegral Inner MembraneBBinoutout/inIM (predicted)Integral Membrane ProteinSRPSECpseudogene (Ecogene), pseudogene (Uniprot)nonoN620.118TMH01.01241-21No27-50iCytoplasmicMembrane9
YPJK_ECOLIP52134
ypjK
b2635 JW5888 integral IM protein (SEC in IM), YpjKIntegral Inner MembraneBBinoutout/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN460.265TMH05.94794-31Yn3-14c22/23o46-68iCytoplasmicMembrane10
CYOC_ECOLIP0ABJ3
cyoC
b0430 JW0420 integral IM protein (SEC in IM), Cytochrome bo(3) ubiquinol oxidase subunit 3 CyoCIntegral Inner Membrane PMID: 11017202 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN140.175TMH03.44396-55Ni29-50o70-91i98-118o138-165i177-201oCytoplasmicMembrane10
CYOD_ECOLIP0ABJ6
cyoD
b0429 JW0419 integral IM protein (SEC in IM), Cytochrome bo(3) ubiquinol oxidase subunit 4 CyoDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN440.15TMH014.0581-33No18-37i44-68o80-100iCytoplasmicMembrane10
CYDA_ECOLIP0ABJ9
cydA

cyd-1

cyd-1b0733 JW0722 integral IM protein (SEC in IM), Cytochrome bd-I ubiquinol oxidase subunit 1 CydAIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15013751 PMID: 16079137 PMID: 15919657 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN370.106TMH09.54728-99No20-42i54-71o91-116i128-151o184-208i220-238o388-409i421-444o471-494iCytoplasmicMembrane10
CYDB_ECOLIP0ABK2
cydB

cyd-2

cyd-2b0734 JW0723 integral IM protein (SEC in IM), Cytochrome bd-I ubiquinol oxidase subunit 2 CydBIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15013751 PMID: 16079137 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN290.118TMH010.5085-88Ni9-28o77-99i119-141o161-181i202-226o264-283i290-315o335-360iCytoplasmicMembrane10
YFJT_ECOLIP52135
yfjT
b2637 JW2618 periplasmic protein (SEC through IM), YfjTPeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoY240.673SpI2319.2157-00Yn9-19c23/24oUnknown222
YJJZ_ECOLIP55914
yjjZ
b4567 JW5797 integral IM protein (SEC in IM), YjjZIntegral Inner MembraneBBpolyin/outIM (predicted)Integral Membrane ProteinSRPSECyesyesN260.223TMH09.90172-33Ni7-25o31-48i55-75oCytoplasmicMembrane919
BLR_ECOLIP56976
blr
b4409 JW5963 integral IM protein (SEC in IM), Divisome-associated BlrIntegral Inner Membrane Small membrane protein detected experimentally.PMID: 19121005 PMID: 10931331 PMID: 22885295Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN270.106CYT0-0.200913-11Ni7-26oCytoplasmicMembrane10
CSGB_ECOLIP0ABK7
csgB
b1041 JW1024 Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Minor curlin subunit CsgBPeripheral outer membrane protein facing the extra-cellular spaceCurli component. CsgB localization requires at least one component of the curli secretion apparatus, CsgF. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041PMID: 16704339 PMCID: PMC2630086 PMID: 21645131 PMID: 19131513 PMID: 9383186 PMID: 9312041Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbrium Cell surface appendage (Experimental)CurliSECCurliyesyesY220.53SpI2116.29-00Yn5-13c21/22oExtracellular9
NRFA_ECOLIP0ABK9
nrfA
b4070 JW4031 periplasmic protein (SEC through IM), Cytochrome c-552 NrfAPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 793493 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY270.665SpI2614.744-00Yn11-21c26/27oPeriplasmic1025
NRFB_ECOLIP0ABL1
nrfB

yjcI

yjcIb4071 JW4032 periplasmic protein (SEC through IM), Cytochrome c-type NrfBPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 15280383 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECnoyesY330.498SpI256.62799-00Yn7-18c23/24oPeriplasmic916
NAPB_ECOLIP0ABL3
napB

yejY

yejYb2203 JW5367 periplasmic protein (SEC through IM), nitrate reductase, electron transfer subunit NapBPeriplasmic Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 10548535 PMID: 17130127 PMID: 11844760 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY280.825SpI2714.6891-00Yn9-20c27/28oPeriplasmic10
NAPC_ECOLIP0ABL5
napC

yejX

yejXb2202 JW2190 integral IM protein (SEC in IM), Cytochrome c-type NapCIntegral Inner Membrane Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 Electrons are fed into the NapAB dimer from a membrane-anchored tetra-heme cytochrome c,encoded by the napC gene. PMID: 8830238 PMID: 12186631PMID: 11844760 PMID: 8830238 PMID: 12186631Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane; Single-pass membrane proteinPeriplasmic with N-terminal membrane anchor (Experimental)SRPSECyesyesN520.116TMH06.01984-11Ni21-44oCytoplasmicMembrane1014
YMGF_ECOLIP58034
ymgF
b4520 JW1156 integral IM protein (SEC in IM), YmgFIntegral Inner Membrane PMID: 18978050Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN370.129TMH00.559611-22Ni12-31o37-59iCytoplasmicMembrane924
YOHJ_ECOLIP60632
yohJ
b2141 JW2129 integral IM protein (SEC in IM), UPF0299 YohJIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN300.138TMH013.9988-44Ni12-32o38-55i67-86o92-113iCytoplasmicMembrane1015
CCMB_ECOLIP0ABL8
ccmB

yejV

yejVb2200 JW2188 integral IM protein (SEC-YidC in IM), Heme exporter B CcmBIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN200.11TMH09.26722-66Ni21-39o45-70i82-103o123-145i157-180o192-215iCytoplasmicMembrane104
CCMC_ECOLIP0ABM1
ccmC

yejT yejU

yejT yejUb2199 JW2187 integral IM protein (SEC-YidC in IM), Heme exporter C CcmCIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. CcmC, CcmE, and heme interact directly with each other, establishing a periplasmic heme delivery pathway for cytochrome c maturation. PMID: 11384983PMID: 22108384 PMID: 15919996 PMID: 11384983Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCnoyesN290.15TMH014.9856-66Ni21-43o63-84i91-113o133-149i161-182o202-221iCytoplasmicMembrane10
CCMH_ECOLIP0ABM9
ccmH

yejP

yejPb2194 JW2182 integral IM protein (SEC in IM), Cytochrome c-type biogenesis CcmHIntegral Inner Membrane Might be is co-translationally translocated through Sec translocon. CcmH is a periplasm-resident oxidoreductase anchored to the inner membrane by two transmembrane segments. Both N- and C-terminal domains are located and function in the periplasmic compartment. PMID: 22789558 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 15838024 PMID: 15919996 PMID: 22789558Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesY190.795SpI188.87226-32Yn3-13c18/19o102-120i147-166oCytoplasmicMembrane1041
KDGL_ECOLIP0ABN1
dgkA
b4042 JW4002 integral IM protein (SEC in IM), Diacylglycerol kinase DgkAIntegral Inner Membrane PMID: 15919996 PMID: 2984194 PMID: 8071224Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN540.112CYT0-0.200913-23Ni30-51o57-80i100-121oCytoplasmicMembrane1031
DCUA_ECOLIP0ABN5
dcuA

genA

genAb4138 JW5735 integral IM protein (SEC in IM), Anaerobic C4-dicarboxylate transporter DcuAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN280.186TMH07.74612-1111No6-38i45-66o86-109i130-152o164-190i225-243o255-277i289-306o326-345i357-381o410-431iCytoplasmicMembrane10
YBJM_ECOLIP64439
ybjM
b0848 JW0832 integral IM protein (SEC in IM), YbjMIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN340.187TMH012.0012-44Ni7-28o40-57i64-83o95-117iCytoplasmicMembrane1052
YCEO_ECOLIP64442
yceO
b1058 JW1045 integral IM protein (SEC in IM), YceOIntegral Inner Membrane Small membrane protein detected experimentally.PMID: 19121005Integral Inner MembraneBBinoutin/outIM (predicted)Membrane anchoredSRPSECnoyesN160.097CYT0-0.200913-11Ni25-45oCytoplasmicMembrane9
DCUB_ECOLIP0ABN9
dcuB

genF

genFb4123 JW4084 integral IM protein (SEC in IM), Anaerobic C4-dicarboxylate transporter DcuBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN680.125TMH08.74519-1110Yn2-13c17/18o27-44i51-71o91-118i139-162o174-195i236-254o266-288i300-318o342-372i421-445oCytoplasmicMembrane10
DCUC_ECOLIP0ABP3
dcuC
b0621 JW0613/JW0616 integral IM protein (SEC in IM), Anaerobic C4-dicarboxylate transporter DcuCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN560.117TMH00.537839-1212Ni5-22o28-46i79-98o118-144i156-175o195-218i238-260o266-288i309-333o339-363i406-424o436-458iCytoplasmicMembrane103
DEDA_ECOLIP0ABP6
dedA
b2317 JW2314 integral IM protein (SEC-YidC in IM) of DedA family, DedAIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF.PMID: 22108384 PMID: 15919996 PMID: 23086209Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN200.121CYT0-0.200913-44Ni27-49o69-92i156-178o190-208iCytoplasmicMembrane10
YIJE_ECOLIP0ABT8
yijE
b3943 JW5557 integral IM protein (SEC in IM), Probable cystine transporter YijE YijEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN300.12TMH07.7108-1010Ni12-29o35-59i71-91o97-118i125-141o153-171i183-205o217-239i246-266o272-289iCytoplasmicMembrane10
EXBB_ECOLIP0ABU7
exbB
b3006 JW2974 integral IM protein (SEC in IM), Biopolymer transport ExbBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.153CYT0-0.200913-33No21-43i132-157o177-198iCytoplasmicMembrane1014
YNAJ_ECOLIP64445
ynaJ
b1332 JW1326 integral IM protein (SEC in IM), YnaJIntegral Inner MembraneBBoutin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN330.241TMH015.6597-21Yn15-26c31/32o55-80iCytoplasmicMembrane929
YNBE_ECOLIP64448
ynbE
b1382 JW1377 OM lipoprotein (SEC through IM, inserted in OM by LOL), YnbEOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesN250.214SpII164.04646Pos+2=T00Yn4-15c24/25oUnknown2
ORTT_ECOLIP64453
ortT

ydcX

ydcXb1445 JW5232 integral IM protein (SEC in IM), Orphan toxin OrtT Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN190.119TMH010.662-22No6-25i37-56oCytoplasmicMembrane9
YNCJ_ECOLIP64459
yncJ
b1436 JW1432 periplasmic protein (SEC through IM), YncJPeriplasmicGN/APeriplasmicSECnoyesY230.752SpI228.63896-00Yn5-16c22/23oUnknown238
TOLQ_ECOLIP0ABU9
tolQ

fii

fiib0737 JW0727 integral IM protein (SEC in IM), Tol-Pal system TolQ Integral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. PMID: 8300535 PMID: 8331075PMID: 9008159 PMID: 15919996 PMID: 8300535 PMID: 8331075Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRP (exp)SECyesyesN340.244TMH02.05597-33No15-35i139-159o171-192iCytoplasmicMembrane109
SFMA_ECOLIP0ABW5
sfmA
b0530 JW0519 Fimbrial periplasmic protein (SEC through IM), SfmAPeriplasmicPotential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (41 81) PMID: 19820722 PMID: 20345943 PeriplasmicGN/AFimbrium PeriplasmicfimbriaeSECCUyesyesY230.832SpI2221.4596-00Yn6-17c22/23oExtracellular1083
FLGB_ECOLIP0ABW9
flgB

fla FII flbA

fla FII flbAb1073 JW1060 Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgBPeriplasmicProximal rod protein. PMID: 12730325 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 15136044PeriplasmicGN/ABacterial flagellum basal body Periplasmic (Experimental)flagellumFlagellum FlayesyesN340.201CYT0-0.200913-00NiPeriplasmic1093
FLGC_ECOLIP0ABX2
flgC

fla FIII flaW

fla FIII flaWb1074 JW1061 Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgCPeriplasmicProximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGN/ABacterial flagellum basal bodyPeriplasmic (Experimental)flagellumFlagellum FlayesyesN320.201CYT0-0.200913-00NiPeriplasmic1043
FLGG_ECOLIP0ABX5
flgG

fla FVII flaL

fla FVII flaLb1078 JW1065 Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgGPeriplasmicDistal rod protein PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGN/ABacterial flagellum basal bodyPeriplasmic (Experimental)flagellumFlagellum FlayesyesN240.123CYT0-0.200913-00NiExtracellular1078
YDHI_ECOLIP64471
ydhI
b1643 JW1635 integral IM protein (SEC in IM), YdhIIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN320.128TMH010.2168-22No20-44i56-77oCytoplasmicMembrane943
YDJM_ECOLIP64481
ydjM
b1728 JW5281 integral IM protein (SEC in IM), YdjMIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN280.333CYT0-0.200913-04Yn7-17c22/23o32-48i69-90o110-128i149-166oCytoplasmicMembrane1015
SURA_ECOLIP0ABZ6
surA
b0053 JW0052 periplasmic protein (SEC through IM), Chaperone SurAPeriplasmicPMID: 21370981 PMID: 19525348 PMID: 9298646 PMID: 22030350 PMID: 14506253PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY210.795SpI2015.5107-00Yn7-15c20/21oPeriplasmic1086
BAMD_ECOLIP0AC02
bamD

yfiO

yfiOb2595 JW2577 OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamDOuter Membrane LipoproteinPart of bam complex. Forms subcomplex with BamCE. Outer membrane protein related to resistance to ampicillin and tetracycline. YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 16372265PMID: 16372265 PMID: 15174130 PMID: 16824102 PMID: 21823654 PMID: 21139201 PMID: 21370981 PMID: 12488465 PMID: 15911532 PMID: 16079137 PMID: 20932056Outer Membrane LipoproteinISoluble IM FractionCell outer membrane Outer membrane Lipoprotein(experimental)SECLOLyesyesY240.171SpII1927.0165Pos+2=S00Yn7-17c22/23oOuterMembrane1022
FLIP_ECOLIP0AC05
fliP

flaR

flaRb1948 JW1932 Flagellar integral IM protein (SEC in IM), biosynthetic FliPIntegral Inner Membrane A cleavable signal peptide is required for the full function of FliP protein.PMID: 15194505 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinflagellumSRPSECnoyesY220.692TMH013.015-54Yn4-15c21/22o45-74i86-105o185-211i223-244oCytoplasmicMembrane10
FOCA_ECOLIP0AC23
focA

ycaE

ycaEb0904 JW0887 integral IM protein (SEC in IM), Probable formate transporter 1 FocAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN190.124TMH01.35944-66Ni35-55o75-98i118-141o161-180i192-212o255-277iCytoplasmicMembrane10
YEAQ_ECOLIP64485
yeaQ
b1795 JW1784 integral IM protein (SEC in IM), UPF0410 YeaQIntegral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN180.108TMH015.2932-33No6-21i28-52o58-77iCytoplasmicMembrane10
YOAF_ECOLIP64493
yoaF
b1793 JW1782 OM lipoprotein (SEC through IM, inserted in OM by LOL), YoaFOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesY240.245SpII1620.7594Pos+2=S00Yn4-15c23/24oUnknown290
YEBO_ECOLIP64499
yebO
b1825 JW1814 integral IM protein (SEC in IM), YebOIntegral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell membrane Membrane anchoredSRPSECyesyesN360.143CYT0-0.200913-11No12-32iUnknown246
YEBY_ECOLIP64506
yebY
b1839 JW1828 periplasmic protein (SEC through IM), YebYPeriplasmic Identified DsbA substrate PMID: 19953543PMID: 19953543PeriplasmicGN/APeriplasmicSECDSBAyesyesY210.862SpI2019.6801-10Yn5-12c20/21oUnknown235
NIRC_ECOLIP0AC26
nirC
b3367 JW3330 integral IM protein (SEC in IM), Nitrite transporter NirC NirCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN350.428TMH04.72286-66Ni25-47o59-80i101-126o146-169i181-206o226-248iCytoplasmicMembrane10
FTSX_ECOLIP0AC30
ftsX

ftsS

ftsSb3462 JW3427 integral IM protein (SEC in IM), Cell division FtsXIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 3323846 PMID: 10048040 PMID: 14729705 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN390.105CYT0-0.200913-44No72-95i214-240o272-290i323-342oCytoplasmicMembrane1023
DHSD_ECOLIP0AC44
sdhD
b0722 JW0712 integral IM protein (SEC in IM), Succinate dehydrogenase hydrophobic anchor subunit SdhDIntegral Inner Membrane PArt of colisuccinate dehydrogenase (SQR) composed of two hydrophilic subunits, a flavoprotein (SdhA) and iron-sulfur protein (SdhB) subunit, and two hydrophobic membrane anchor subunits, SdhC and SdhD.PMID: 11266632 PMID: 12560550 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN390.133TMH010.7371-33Ni17-39o59-80i92-113oCytoplasmicMembrane1044
KDTA_ECOLIP0AC75
waaA

kdtA

kdtAb3633 JW3608 integral IM protein (SEC in IM), 3-deoxy-D-manno-octulosonic acid transferase WaaAIntegral Inner Membrane Involved in lipopolysaccharide biosynthesis PMID: 2651435 PMID: 1577828. Embedded in the cytoplasmic face of the inner membrane, WaaA catalyzes the transfer of 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) to the lipid A precursor of LPS (in Aquifex aeolicus) PMID: 22474366.PMID: 2651435 PMID: 1577828 PMID: 15866922 PMID: 22474366 PMID: 19275539Integral Inner MembraneBBinout/inIM (predicted)Cell inner membrane CytoplasmicSRPSECyesyesN640.121CYT0-0.200913-01No6-23iUnknown215
MGRB_ECOLIP64512
mgrB

yobG

yobGb1826 JW1815 integral IM protein (SEC in IM), PhoP/PhoQ regulator MgrB Integral Inner Membrane Small membrane protein detected experimentally. Localization and bacterial two-hybrid studies suggest that MgrB resides in the inner-membrane and interacts directly with PhoQ.PMID: 19121005 PMID: 20041203Integral Inner MembraneBBinoutin/outN/ACell inner membrane PeriplasmicSECnoyesN220.24SpI2110.2239-10Yn6-17c21/22oCytoplasmicMembrane953
RCNB_ECOLIP64534
rcnB

yohN

yohNb2107 JW5346 periplasmic protein (SEC through IM), Nickel/cobalt homeostasis RcnB PeriplasmicPMID: 21665978PeriplasmicGN/APeriplasm PeriplasmicSECnoyesY220.81SpI2123.7077-10Yn8-16c21/22oUnknown279
YEIS_ECOLIP64536
yeiS
b2145 JW5359 integral IM protein (SEC in IM), YeiSIntegral Inner MembraneB-IM (predicted)Membrane Integral Membrane ProteinSRPSECnoyesN240.124TMH02.78571-22No6-24i54-77oCytoplasmicMembrane957
WECA_ECOLIP0AC78
wecA

rfe

rfeb3784 JW3758 integral IM protein (SEC in IM), Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase WecAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN290.115CYT0-0.200913-1111No6-24i45-66o72-89i101-119o131-149i161-179o185-206i213-232o244-263i294-315o321-342iCytoplasmicMembrane10
GNTP_ECOLIP0AC94
gntP

yjiB

yjiBb4321 JW4284 integral IM protein (SEC in IM), High-affinity gluconate transporter GntPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN430.178TMH07.17039-1312Ni7-23o29-49i61-79o99-132i139-157o177-201i222-249o261-280i301-320o340-373i380-402o422-446iCytoplasmicMembrane10
GNTU_ECOLIP0AC96
gntU
b4476 JW5686 integral IM protein (SEC in IM), Low-affinity gluconate transporter GntUIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECpseudogene (Ochman & Davalos, 2006)yesyesN310.139TMH010.5274-1312Ni5-22o28-47i59-80o100-128i140-163o175-197i226-250o262-282i303-320o340-373i385-408o428-445iCytoplasmicMembrane10
SATP_ECOLIP0AC98
satP

yaaH

yaaHb0010 JW0009 integral IM protein (SEC in IM), Succinate-acetate/proton symporter SatP Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN340.122TMH09.07966-66Ni12-31o37-56i63-84o96-116i123-143o149-169iCytoplasmicMembrane10
HEMY_ECOLIP0ACB7
hemY
b3802 JW3774 integral IM protein (SEC in IM), HemYIntegral Inner Membrane Trigger factor-sensitive aggregation.PMID: 19737520 PMID: 15919996Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRP,TFSECyesyesN230.235TMH010.5296-21Yn4-17c26/27o42-62iCytoplasmicMembrane1019
YPEC_ECOLIP64542
ypeC
b2390 JW2387 periplasmic protein (SEC through IM), YpeCPeriplasmicGN/APeriplasmicSECnoyesY220.729SpI2114.9819-00Yn4-15c21/22oUnknown214
YFGG_ECOLIP64545
yfgG
b2504 JW5399 integral IM protein (SEC in IM), YfgGIntegral Inner MembraneBBinoutin/outN/AMembrane Membrane anchoredSRPSECnoyesN410.141TMH03.8997-10Yn20-31c39/40oCytoplasmicMembrane9
YFIR_ECOLIP64548
yfiR
b2603 JW2584 periplasmic protein (SEC through IM), YfiRPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasm PeriplasmicSECyesyesY230.684SpI2213.4131-00Yn7-17c22/23oUnknown621
MBHM_ECOLIP0ACE0
hybC
b2994 JW2962 periplasmic peripheral IM protein (TAT via piggyback through IM), Hydrogenase-2 large chain HybCPeripheral inner membrane protein facing the periplasmThe protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system (piggyback). PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544PMID: 10224080 PMID: 3516690 PMID: 8772179 PMID: 9738917 PMID: 25664544Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane; Peripheral membrane proteinPeriplasmic (Experimental)TAT (piggyback)yesyesN340.139CYT0-0.200913-00NiCytoplasmicMembrane1028
LPXL_ECOLIP0ACV0
lpxL

htrB waaM

htrB waaMb1054 JW1041 integral IM protein (SEC in IM), Lipid A biosynthesis lauroyltransferase LpxLIntegral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN200.104TMH01.65586-12Ni12-30o36-52iCytoplasmicMembrane1011
LPXP_ECOLIP0ACV2
lpxP

ddg

ddgb2378 JW2375 integral IM protein (SEC-YidC in IM), Lipid A biosynthesis palmitoleoyltransferase LpxPIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384Integral Inner MembraneBBinout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSEC-YidCyesyesN490.166TMH01.68704-11No20-46iCytoplasmicMembrane99
LAPA_ECOLIP0ACV4
LapA

yciS

yciSb1279 JW1271 integral IM protein (SEC in IM), polysaccharide assembly A LapAIntegral Inner Membrane YciS (LapA) possibly participates both in lateral elongation of peptidoglycan and septum formation.PMID: 24722986 C-terminal tagging with the alkaline phosphatase and green fluorescent protein in order to define the periplasmic or cytoplasmic locations of the C termini. PMID: 15919996 Heat shock protein involved in the assembly of LPS. In pull-down experiments, LapA and LapB co-purified with LPS, Lpt proteins, FtsH (protease), DnaK, and DnaJ (chaperones). PMID: 25403562PMID: 15919996 PMID: 25403562Integral Inner MembraneBBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN230.34TMH011.7404-21Yn4-16c21/22o45-67iCytoplasmicMembrane1036
AlaE_ECOLIP64550
alaE

ygaW

ygaWb2670 JW2645 integral IM protein (SEC in IM), L-alanine exporter AlaE Integral Inner Membrane PMID: 21531828Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN490.117TMH06.24909-44Ni12-36o42-62i82-104o110-131iCytoplasmicMembrane1012
YQJE_ECOLIP64585
yqjE
b3099 JW3070 integral IM protein (SEC in IM), YqjEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN620.126CYT0-0.200913-22Ni50-76o82-104iCytoplasmicMembrane10
YHAH_ECOLIP64590
yhaH
b3103 JW3074 integral IM protein (SEC in IM), YhaHIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.122TMH08.08221-33No23-40i52-70o82-103iCytoplasmicMembrane108
YDGC_ECOLIP0ACX0
ydgC
b1607 JW1599 integral IM protein (SEC in IM), YdgCIntegral Inner Membrane Dual topology membrane protein: can insert into the membrane in both directions. PMID: 15919996PMID: 15919996 PMID: 24688744 PMID: 16429150Integral Inner MembraneBBpolyin/out, out/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.169TMH03.73379-43Yn7-17c21/22o31-47i59-79o85-108iCytoplasmicMembrane1023
YEBS_ECOLIP0AD03
yebS
b1833 JW1822 integral IM protein (SEC in IM), Intertransport YebS Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN570.112CYT0-0.200913-88Ni66-84o120-141i162-179o185-208i266-291o311-339i360-378o384-406iCytoplasmicMembrane10
BTSS_ECOLIP0AD14
btsS

yehU

yehUb2126 JW5353 integral IM protein (SEC in IM), Sensor histidine kinase BtsSIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN220.144TMH06.36838-66Ni7-24o44-62i74-93o105-129i141-163o175-195iCytoplasmicMembrane102
YOHK_ECOLIP0AD19
yohK
b2142 JW2130 integral IM protein (SEC in IM), YohKIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN240.211TMH01.42711-64Yn7-18c23/24o33-52i91-112o132-162i206-230oCytoplasmicMembrane10
YEJM_ECOLIP0AD27
yejM

yejN

yejNb2188 JW2176 integral IM protein (SEC in IM), YejMIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN680.133TMH014.6981-55Ni21-39o51-73i85-103o135-157i169-191oCytoplasmicMembrane102
YHAI_ECOLIP64592
yhaI
b3104 JW3075 integral IM protein (SEC in IM), YhaIIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.264TMH00.961473-33Ni23-44o50-68i80-98oCytoplasmicMembrane1028
YHCN_ECOLIP64614
yhcN
b3238 JW5540 periplasmic protein (SEC through IM), YhcNPeriplasmicGN/APeriplasm PeriplasmicSECyesyesY230.834SpI2222.7033-00Yn7-18c22/23oUnknown2
YHDU_ECOLIP64619
yhdU
b3263 JW3231 integral IM protein (SEC in IM), YhdUPotential Signal PeptideIntegral Inner MembraneBBinoutin/outIM (predicted)Cell inner membrane Membrane anchoredSRPSECnoyesN310.117TMH00.197917-10Yn7-18c30/31oCytoplasmicMembrane982
YFCA_ECOLIP0AD30
yfcA
b2327 JW2324 integral IM protein (SEC in IM), Probable transporter YfcA Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN540.218TMH011.5476-78Ni7-28o53-73i85-105o111-127i139-158o164-183i195-217o237-255iCytoplasmicMembrane10
PGPC_ECOLIP0AD42
pgpC

yfhB

yfhBb2560 JW5408 integral IM protein (SEC in IM), Phosphatidylglycerophosphatase C PgpCIntegral Inner Membrane Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 25403562Integral Inner MembraneBBinout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECYfgMnoyesN520.106CYT0-0.200913-11No36-54iCytoplasmicMembrane98
YFHG_ECOLIP0AD44
yfhG
b2555 JW2539 OM lipoprotein (TAT-SEC through IM, inserted in OM by LOL), YfhGOuter Membrane LipoproteinTAT secretion system.PMID: 17218314Outer Membrane LipoproteinIN/AOuter membrane LipoproteinTAT,SECLOLyesyesY230.159SpII256.96106Pos+2=V00Yn13-24c29/30oUnknown255
IVY_ECOLIP0AD59
ivy

ykfE

ykfEb0220 JW0210 periplasmic protein (SEC through IM), Inhibitor of vertebrate lysozyme IvyPeriplasmicExperimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 11278658 PMID: 25664544PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECyesyesY290.849SpI2816.6514-00Yn13-23c28/29oPeriplasmic1027
MRDA_ECOLIP0AD65
mrdA

pbpA

pbpAb0635 JW0630 integral IM protein (SEC in IM), PG D,D-transpeptidase MrdA Integral Inner Membrane PMID: 1103132 PMID: 15919657Integral Inner MembraneBBoutin/outIM (predicted), Soluble IM Fraction, Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN370.174CYT0-0.200913-11Ni21-40oCytoplasmicMembrane98
YHDV_ECOLIP64622
yhdV
b3267 JW3235 OM lipoprotein (SEC through IM, inserted in OM by LOL), YhdVOuter Membrane LipoproteinProven signal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIIM (predicted)Cell membrane Outer membrane Lipoprotein (Experimental)SECLOLnoyesY210.268SpII1614.2179Pos+2=A11Yn4-15c20/21o50-71iUnknown230
YHFL_ECOLIP64627
yhfL
b3369 JW3332 OM lipoprotein (SEC through IM, inserted in OM by LOL), YhfLOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 21890895Outer Membrane LipoproteinIN/AMembrane Outer membrane Lipoprotein (Experimental)SECLOLnoyesN220.162SpII1915.9797Pos+2=T00Yn7-17c22/23oUnknown2
YHFU_ECOLIP64631
yhfU
b3378 JW5697 integral IM protein (SEC in IM), YhfUIntegral Inner MembraneBBinoutout/inN/AMembrane anchoredSRPSECnoyesN370.114CYT0-0.200913-11No47-69iUnknown290
FTSI_ECOLIP0AD68
ftsI

pbpB

pbpBb0084 JW0082 integral IM protein (SEC in IM), PG D,D-transpeptidase FtsI Integral Inner Membrane The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein. Localizes to potential division sites in filamentous cells. Interacts directly with FtsN and ZapA. The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex.PMID: 11703663 PMID: 20497333 PMID: 20847002Integral Inner MembraneBBoutin/outSoluble IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN370.141SpI453.45111-11Ni20-44oCytoplasmicMembrane922
LIVJ_ECOLIP0AD96
livJ
b3460 JW3425 periplasmic protein (SEC through IM), Leu/Ile/Val-binding LivJPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 19953543 PMID: 9298646 PMID: 22030350 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.832SpI2320.6668-00Yn7-18c23/24oPeriplasmic1096
BRNQ_ECOLIP0AD99
brnQ
b0401 JW0391 integral IM protein (SEC-YidC in IM), Branched-chain amino acid transport system 2 carrier BrnQIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN250.304TMH015.0757-1212Ni12-29o41-64i76-98o118-138i150-171o191-212i224-246o281-307i314-333o339-359i371-389o409-426iCytoplasmicMembrane10
TESA_ECOLIP0ADA1
tesA

apeA pldC

apeA pldCb0494 JW0483 periplasmic protein (SEC through IM), Thioesterase 1/protease 1/lysophospholipase L1 TesAPeriplasmicPMID: 8098033PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECnoyesN270.484SpI2611.0906-10Yn12-22c26/27oPeriplasmic1040
HOFN_ECOLIP64634
hofN

yrfC

yrfCb3394 JW3357 integral IM protein (SEC in IM), DNA utilization HofNIntegral Inner MembraneBBoutin/outN/ACell inner membrane Membrane anchoredSRPSECyesyesN490.175TMH03.72613-11Ni21-40oCytoplasmicMembrane922
GHOT_ECOLIP64646
ghoT

yjdO

yjdOb4559 JW5732 integral IM protein (SEC in IM), Toxin GhoT Integral Inner Membrane Toxin-antitoxin system Ghot-GhoS.In vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA.PMID: 22941047Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN330.1TMH09.94204-22Ni7-26o32-56iCytoplasmicMembrane9
NLPD_ECOLIP0ADA3
nlpD
b2742 JW2712 OM lipoprotein (SEC through IM), inserted in OM by LOL), Murein hydrolase activator NlpD Outer Membrane LipoproteinProven signal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell inner membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY250.134SpII2521.9047Pos+2=S00Yn13-24c29/30oOuterMembrane985
YAJG_ECOLIP0ADA5
yajG
b0434 JW0424 OM lipoprotein (SEC through IM, inserted in OM by LOL), YajGOuter Membrane LipoproteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 9298646PMID: 15911532 PMID: 20932056 PMID: 9298646Outer Membrane LipoproteinISoluble IM FractionCell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY200.403SpII1719.0402Pos+2=A00Yn5-15c19/20oUnknown2
OSME_ECOLIP0ADB1
osmE

anr

anrb1739 JW1728 OM lipoprotein (SEC through IM), inserted in OM by LOL), Osmotically-inducible putative OsmE Outer Membrane LipoproteinProven signal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 21541338 PMID: 10806384 PMID: 25664544Outer Membrane LipoproteinISoluble IM FractionCell inner membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY240.207SpII2020.0226Pos+2=T10Yn7-18c23/24oUnknown298
ECNB_ECOLIP0ADB7
ecnB

yjeU

yjeUb4411 JW4108 OM lipoprotein (SEC through IM), inserted in OM by LOL), Entericidin B EcnBOuter Membrane LipoproteinPotentially toxic protein. Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130PMID: 15174130 PMID: 19121005 PMID: 20932056 PMID: 21541338Outer Membrane LipoproteinISoluble IM FractionCell membrane; Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY220.27SpII2117.5085Pos+2=N10Yn5-15c20/21oCytoplasmicMembrane10
LPTE_ECOLIP0ADC1
lptE

rlpB

rlpBb0641 JW0636 OM lipoprotein (SEC through IM), inserted in OM by LOL), LPS-assembly LptE Outer Membrane LipoproteinProven signal peptidase II substrate. PMID: 16861298PMID: 15174130 PMID: 20932056 PMID: 16861298 PMID: 22901060Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN250.125SpII1817.3262Pos+2=G00Yn4-15c20/21oOuterMembrane1080
YQHA_ECOLIP67244
yqhA
b3002 JW2971 integral IM protein (SEC in IM), UPF0114 YqhAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell membrane Integral Membrane Protein (Experimental)SRPSECyesyesN320.15TMH06.53453-34No12-32i53-75o109-126i138-156oCytoplasmicMembrane1015
YOBD_ECOLIP67601
yobD
b1820 JW1809 integral IM protein (SEC in IM), UPF0266 YobDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.285TMH03.32601-23No6-24i45-62o68-87iCytoplasmicMembrane10
LOLC_ECOLIP0ADC3
lolC

ycfU

ycfUb1116 JW5161 integral IM protein (SEC in IM), Lipoprotein-releasing system TM protein LolCIntegral Inner Membrane PMID: 15276320 PMID: 10783239Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN190.179TMH02.37377-45No25-48i269-290o310-336i343-360o366-385iCytoplasmicMembrane1029
LPTG_ECOLIP0ADC6
lptG

yjgQ

yjgQb4262 JW5760 integral IM protein (SEC in IM), polysaccharide export system permease LptG Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN360.105TMH04.7678-66Ni12-33o66-84i105-125o279-298i305-322o334-356iCytoplasmicMembrane109
YJJP_ECOLIP0ADD5
yjjP
b4364 JW5796 integral IM protein (SEC in IM), YjjPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN280.178CYT0-0.200913-35Ni114-135o141-158i170-189o195-214i226-252oCytoplasmicMembrane1016
TAMA_ECOLIP0ADE4
tamA

yftM ytfM

yftM ytfMb4220 JW4179 OM b-barrel protein (SEC through IM, inserted in OM by BAM and TAM), Translocation and assembly module subunit TamAOuter Membrane b-barrel proteinPart of the translocation and assembly module (TAM). TAM system promotes efficient secretion of autotransporters in proteobacteria.PMID: 17214547 PMID: 22466966Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel proteinSECBAMyesyesY220.861SpI2111.5811-00Yn7-15c21/22oOuterMembrane10
YGFZ_ECOLIP0ADE8
ygfZ

yzzW

yzzWb2898 JW2866 cytoplasmic & periplasmic protein (SEC through IM), tRNA-modifying YgfZ Cytoplasmic, PeriplasmicSubcellular localization analysis of cytotoxic necrotizing factor 1 <a href="http://www.uniprot.org/uniprot/Q1R2U0">(CNF1)</a> demonstrated that YgfZ, a periplasmic protein, contributes to secretion of CNF1 (a normally cytoplasmic protein) into outer-membrane vesicles (OMVs) in pathogenic E.coli strain RS218 O18:K1:H7 PMID: 22174383. PMID: 9298646PMID: 22174383 PMID: 9298646Cytoplasmic, PeriplasmicA, GN/ACytoplasm Cytoplasmic (Experimental)SECyesyesN310.252CYT0-0.200913-00NiUnknown282
MDTJ_ECOLIP69212
mdtJ

ydgF

ydgFb1600 JW1592 integral IM protein (SEC in IM), Spermidine export MdtJ Integral Inner Membrane PMID: 15919996 PMID: 24688744Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN260.125TMH07.68505-43Yn3-13c21/22o31-52i59-80o86-102iCytoplasmicMembrane1036
PGAD_ECOLIP69432
pgaD

ycdP

ycdPb1021 JW1006 integral IM protein (SEC in IM), Biofilm PGA synthesis PgaDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN380.135TMH010.5144-22Ni16-36o56-74iCytoplasmicMembrane109
YIAF_ECOLIP0ADK0
yiaF
b3554 JW5655 IM lipoprotein (SEC through IM, inserted in OM by LOL), YiaFUncharacterized protein yiaF - echolocation Occurrence of signal peptide determined by LipoP signal sequence shown based upon lipoP, not possible to determine which membrane based on +2 amino acid rule.Inner Membrane LipoproteinESoluble IM FractionMembrane LipoproteinSECLOLyesyesN280.132SpII2510.3533Pos+2=F10Yn10-21c26/27oUnknown267
NEPI_ECOLIP0ADL1
nepI

yicM

yicMb3662 JW5938 integral IM protein (SEC in IM), Purine ribonucleoside efflux pump NepI NepIIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN480.151TMH08.80109-1212Ni21-44o56-75i87-106o112-133i145-164o176-196i217-239o254-275i282-301o307-329i341-363o369-389iCytoplasmicMembrane10
YIFL_ECOLIP0ADN6
yifL
b4558 JW3781 OM lipoprotein (SEC through IM, inserted in OM by LOL), YifLOuter Membrane LipoproteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 16397293PMID: 15911532 PMID: 16397293Outer Membrane LipoproteinISoluble IM FractionCell membrane Membrane Lipoprotein (Experimental)SECLOLnoyesY240.159SpII1927.2584Pos+2=G10Yn7-18c23/24oUnknown2
YIGM_ECOLIP0ADP5
yigM
b3827 JW3803 integral IM protein (SEC in IM), Biotin transporter YigMIntegral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN200.164TMH06.74192-109Yn2-13c19/20o29-47i59-81o87-105i112-130o136-158i170-190o202-222i234-253o259-276iCytoplasmicMembrane10
YIID_ECOLIP0ADQ2
yiiD
b3888 JW3859 integral IM protein (SEC in IM), YiiDIntegral Inner Membrane Copurifies with trigger factor in pull down assays.PMID: 19737520Integral Inner MembraneBBinoutout/inIM (predicted)CytoplasmicSRP,TFSECnoyesN290.101CYT0-0.200913-01No219-240iCytoplasmic853
PTXB_ECOLIP69789
glvB

glvC

glvCb3682 JW3659 integral IM protein (SEC in IM), Phosphotransferase enzyme IIB component GlvB Integral Inner MembraneBBinout/inIM (predicted)Cell membrane Membrane anchoredSRPSECpseudogene (Ochman & Davalos, 2006)yesnoN450.17CYT0-0.200913-11No12-31iCytoplasmicMembrane933
YAAX_ECOLIP75616
yaaX
b0005 JW0004 periplasmic protein (SEC through IM), YaaXPeriplasmicGN/APeriplasmicSECnoyesY240.841SpI2319.693-10Yn6-17c23/24oUnknown277
AFUB_ECOLIP75681
afuB

fbpB

fbpBb0263 JW0255 integral IM protein (SEC in IM), Putative ferric transport system permease-like AfuB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN460.154CYT0-0.200913-22Ni33-56o76-101iCytoplasmicMembrane930
RCLC_ECOLIP75685
rclC

ykgB

ykgBb0301 JW5038 integral IM protein (SEC in IM), RclCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN410.117TMH00.0510112-34Ni12-32o87-105i112-133o153-175iCytoplasmicMembrane10
YQAA_ECOLIP0ADR0
yqaA
b2689 JW2664 integral IM protein (SEC in IM), YqaAIntegral Inner Membrane Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF. PMID: 15919996PMID: 15919996 PMID: 23086209Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN190.233TMH05.40517-43Yn4-12c17/18o41-65i86-110o116-141iCytoplasmicMembrane104
YGDD_ECOLIP0ADR2
ygdD
b2807 JW2778 integral IM protein (SEC in IM), UPF0382 YgdDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN250.249TMH014.4685-43Yn7-20c24/25o40-61i68-91o97-121iCytoplasmicMembrane10
YGGN_ECOLIP0ADS9
yggN
b2958 JW2925 periplasmic protein (SEC through IM), YggNPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535PMID: 14726535 PMID: 19953543PeriplasmicGN/APeriplasmic(experimental)SECDSBAyesyesY210.797SpI2021.2471-00Yn6-16c20/21oUnknown280
YGIM_ECOLIP0ADT8
ygiM
b3055 JW3027 integral IM protein (SEC in IM), YgiMIntegral Inner MembraneBBinoutout/inIM (predicted), Detected in IM FractionMembrane Membrane anchoredSRPSECyesyesY230.542SpI2218.2018-11Yn6-17c22/23o173-195iUnknown212
MLAC_ECOLIP0ADV7
mlaC

yrbC

yrbCb3192 JW3159 periplasmic protein (SEC through IM), Interphospholipid transport system binding MlaC PeriplasmicPMID: 15911532PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECnoyesY220.509SpI2114.0896-00Yn8-16c21/22oUnknown261
LPTC_ECOLIP0ADV9
lptC

yrbK

yrbKb3199 JW3166 integral IM protein (SEC in IM), polysaccharide export system LptC Integral Inner Membrane Echolocation says Signal peptide may be membrane anchored or cleaved and the protein therefore exported (signalP score near threshold)PMID: 18424520 PMID: 20720015Integral Inner MembraneBBoutin/outSoluble IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN260.212SpI257.46611-10Yn7-18c27/28oUnknown251
RCLB_ECOLIP75687
rclB

ykgI

ykgIb0303 JW5039 periplasmic protein (SEC through IM), RclBPeriplasmicGN/APeriplasm PeriplasmicSECnoyesY230.662SpI2216.2846-00Yn6-17c22/23oUnknown2
YAHO_ECOLIP75694
yahO
b0329 JW0321 periplasmic protein (SEC through IM), YahOPeriplasmicGN/APeriplasm PeriplasmicSECnoyesY220.85SpI2113.1005-00Yn8-16c21/22oUnknown285
YBBV_ECOLIP75711
ybbV
b0510 JW0498 integral IM protein (SEC in IM), YbbVIntegral Inner MembraneBBinout/inIM (predicted)Membrane anchoredSRPSECnoyesN610.11CYT0-0.200913-11No15-33iUnknown2
YHCB_ECOLIP0ADW3
yhcB
b3233 JW5539 integral IM protein (SEC in IM), YhcBIntegral Inner Membrane Has been isolated from inner membrane preparations in a complex with one subunit each of CydA and CydB, suggesting that YhcB is in fact a subunit of the cytochrome bd ubiquinol oxidase complex. YhcB was demonstrated to interact with RodZ as well as several other proteins involved in cell shape maintenance and an inner membrane protein YciS of unknown function, using bacterial two-hybrid system.These observations seem to indicate that YhcB is involved in the biogenesis of cell envelope and the maintenance of cell shape together with RodZ. <a href="http:/PMID: 16079137 PMID: 21210718 PMID: 15919657Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECnoyesN210.134CYT0-0.200913-11No6-24iCytoplasmicMembrane983
PPID_ECOLIP0ADY1
ppiD

ybaU

ybaUb0441 JW0431 integral IM protein (SEC in IM), Peptidyl-prolyl cis-trans isomerase D PpiDIntegral Inner Membrane By in vivo and in vitro site-directed cross-linking, it has been demostrated that the periplasmic chaperone PpiD is located in front of the lateral gate of SecY, through which transmembrane domains exit the SecY channel. Blue native PAGE analyses verify the presence of a SecYEG-PpiD complex in native Escherichia coli membranes. PMID: 24951590 Consistent with a coupling between translocation across the SecYEG translocon and folding by periplasmic chaperones, a lack of PpiD retards the release of a translocating outer membrane protein into the periplasm. PMID: 18439025PMID: 16079137 PMID: 9670013 PMID: 24951590 PMID: 18439025Integral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchored (Experimental)SRPSECyesyesN430.199TMH03.55242-11Ni12-35oCytoplasmicMembrane1052
YAJC_ECOLIP0ADZ7
yajC
b0407 JW0397 integral IM protein (SEC in IM), Sec translocon accessory complex subunit YajC Integral Inner Membrane Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 16079137 PMID: 21541338Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN170.156TMH0-0.000234246-11No20-39iCytoplasmicMembrane1041
ACRA_ECOLIP0AE06
acrA

lir mtcA

lir mtcAb0463 JW0452 IM lipoprotein (SEC through IM), inserted in OM by LOL), Multidrug efflux pump subunit AcrA Inner Membrane LipoproteinProven signal peptidase II substrate. PMID: 10894736PMID: 15174130 PMID: 15228545 PMID: 16079137 PMID: 10894736Inner Membrane LipoproteinESoluble IM FractionCell inner membrane Inner membrane lipoprotein (Experimental)SECLOLyesyesY220.194SpII2419.636Pos+2=D00Yn6-16c21/22oCytoplasmicMembrane1064
RZPD_ECOLIP75719
rzpD

ybcT

ybcTb0556 JW5079 integral IM protein (SEC in IM), Prophage Rz endopeptidase RzpD Integral Inner Membrane Required for normal curli ex<x>pression and biofilm development.PMID: 23378572Integral Inner MembraneB-N/APeriplasmicSRPSECProphage Integrase (EcoGene)nonoN230.233SpI223.631-10Yn6-17c22/23oUnknown228
CHIQ_ECOLIP75734
chiQ

ybfN

ybfNb0682 JW0668 OM lipoprotein (SEC through IM, inserted in OM by LOL), ChiQOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY190.487SpII1619.5262Pos+2=A00Yn4-14c18/19oUnknown277
AMPE_ECOLIP0AE14
ampE
b0111 JW0107 integral IM protein (SEC in IM), AmpEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN230.115CYT0-0.200913-44Yn2-12c16/17o40-58i65-85o148-168i266-283oCytoplasmicMembrane103
AMPG_ECOLIP0AE16
ampG
b0433 JW0423 integral IM protein (SEC-YidC in IM), AmpGIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN380.305TMH012.6077-1414Ni15-38o44-61i82-101o107-131i143-165o177-195i225-250o262-281i288-309o321-342i354-376o382-402i423-445o465-485iCytoplasmicMembrane10
APHA_ECOLIP0AE22
aphA

napA yjbP

napA yjbPb4055 JW4015 periplasmic protein (SEC through IM), Class B acid phosphatase AphAPeriplasmicPMID: 9011040 PMID: 9298646PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECnoyesY260.643SpI2514.5914-00Yn7-17c25/26oUnknown2
ARAE_ECOLIP0AE24
araE
b2841 JW2809 integral IM protein (SEC-YidC in IM), Arabinose-proton symporter AraEIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN450.136TMH07.37397-1212Ni23-51o63-84i91-110o116-137i149-167o179-198i258-281o297-317i329-348o360-384i405-422o428-446iCytoplasmicMembrane10
ARAH_ECOLIP0AE26
araH
b4460 JW1887 integral IM protein (SEC-YidC in IM), L-arabinose transport system permease AraH Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCpseudogene (Ochman & Davalos, 2006)noyesN470.147TMH013.0464-910Ni21-42o54-72i79-96o102-124i131-150o170-191i222-240o252-269i276-295o301-317iCytoplasmicMembrane10
YBHM_ECOLIP75769
ybhM
b0787 JW0770 integral IM protein (SEC in IM), YbhMIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN410.18TMH04.77792-77Ni21-41o53-70i77-98o104-129i136-155o161-179i209-229oCytoplasmicMembrane1067
YCAP_ECOLIP75839
ycaP
b0906 JW0889 integral IM protein (SEC in IM), UPF0702 transYcaP Integral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN640.104TMH00.694512-33No18-37i49-69o75-96iCytoplasmic826
ARTM_ECOLIP0AE30
artM
b0861 JW0845 integral IM protein (SEC-YidC in IM), Arginine ABC transporter permease ArtMIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN270.106TMH012.0162-55No20-39i51-70o82-100i155-176o188-211iCytoplasmicMembrane10
ARTQ_ECOLIP0AE34
artQ
b0862 JW0846 integral IM protein (SEC-YidC in IM), Arginine ABC transporter permease ArtQIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN300.173TMH017.1037-45No12-35i42-68o101-118i161-182o202-223iCytoplasmicMembrane1026
YTFL_ECOLIP0AE45
ytfL
b4218 JW4177 integral IM protein (SEC in IM), UPF0053 YtfLIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.622SpI269.49148-43Yn5-18c26/27o56-80i100-119o139-162iCytoplasmicMembrane1048
CITT_ECOLIP0AE74
citT

ybdS

ybdSb0612 JW0604 integral IM protein (SEC in IM), Citrate/succinate antiporter CitTIntegral Inner Membrane PMID: 9696764Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN580.164TMH013.6692-1314Ni7-27o33-50i57-74o94-117i138-155o161-178i190-213o233-259i288-304o310-329i341-361o381-412i424-443o463-486iCytoplasmicMembrane10
GFCC_ECOLIP75883
gfcC

ymcB

ymcBb0985 JW0968 periplasmic protein (SEC through IM), GfcCPeriplasmicGN/APeriplasmicSECyesyesY230.723SpI228.45245-00Yn4-15c22/23oUnknown241
YCDZ_ECOLIP75916
ycdZ
b1036 JW5147 integral IM protein (SEC in IM), YcdZIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN650.232TMH014.4904-55No20-42i49-70o76-94i99-117o123-147iCytoplasmicMembrane10
YMDA_ECOLIP75917
ymdA
b1044 JW1031 periplasmic protein (SEC through IM), YmdAPeriplasmicGN/APeriplasmicSECnoyesY230.463SpI2213.2508-10Yn11-22c28/29oExtracellular936
YCFL_ECOLIP75946
ycfL
b1104 JW1090 OM lipoprotein (SEC through IM, inserted in OM by LOL), YcfLOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesY240.214SpII1819.4253Pos+2=R00Yn5-16c21/22oUnknown270
CPXA_ECOLIP0AE82
cpxA

ecfB eup ssd

ecfB eup ssdb3911 JW3882 integral IM protein (SEC-YidC in IM), Sensor histidine kinase CpxAIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN190.111TMH01.20569-22No6-27i165-184oCytoplasmicMembrane1016
CPXP_ECOLIP0AE85
cpxP

yiiO

yiiOb4484 JW5558 periplasmic peripheral IM protein (SEC through IM), CpxP Peripheral inner membrane protein facing the periplasmPMID: 17904518 PMID: 10972835 PMID: 21317318Peripheral inner membrane protein facing the periplasmF2N/APeriplasm Membrane associated (Experimental)SECpseudogene (Ochman & Davalos, 2006)yesyesY220.806SpI2116.0374-00Yn3-14c21/22oPeriplasmic10104
CSGE_ECOLIP0AE95
csgE
b1039 JW1022 Curli related periplasmic protein (SEC through IM), Curli production assembly/transport component CsgEPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475PMID: 16704339 PMID: 18498364 PMID: 21645131 PMID: 19131513 PMID: 9928475PeriplasmicGN/APeriplasmic (Experimental)CurliSECSURAyesyesY230.789SpI223.89784-00Yn7-17c22/23oUnknown215
CSGF_ECOLIP0AE98
csgF
b1038 JW1021 Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Curli production assembly/transport component CsgFPeripheral outer membrane protein facing the extra-cellular spaceCurli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475PMID: 16704339 PMID: 16522795 PMID: 21645131 PMID: 19131513 PMID: 9928475Peripheral outer membrane protein facing the extra-cellular spaceF4N/ACell outer membrane Periplasmic (Experimental)CurliSECCurliyesyesY200.853SpI1913.9164-10Yn6-14c19/20oExtracellular930
YMFA_ECOLIP75962
ymfA
b1122 JW5164 integral IM protein (SEC in IM), YmfAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN290.212TMH06.47267-22Ni7-28o117-139iCytoplasmicMembrane10
YMFE_ECOLIP75968
ymfE
b1138 JW5166 integral IM protein (SEC in IM), YmfEIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN370.102TMH01.7441-44Ni21-39o59-79i151-173o185-208iCytoplasmicMembrane107
Y1142_ECOLIP75971
b1142
b1142 JW1128 integral IM protein (SEC in IM), Putative b1142 B114Integral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECpseudogene (Uniprot), pseudogene (Ecogene), pseudogene (Ochman & Davalos, 2006)nonoN630.119CYT0-0.200913-22Ni41-62o74-92iUnknown254
CSGG_ECOLIP0AEA2
csgG
b1037 JW1020 Curli related OM lipoprotein & extra-cellular peripheral OM protein (SEC through IM), inserted in OM by LOL), Curli production assembly/transport component CsgGOuter Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceCurli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130PMID: 16704339 PMID: 15174130 PMID: 21645131 PMID: 16420357 PMID: 19131513Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceI, F4N/ACell membrane Outer membrane Lipoprotein (Experimental)CurliSECLOLyesyesY200.544SpII1512.9163Pos+2=L00Yn4-13c18/19oCytoplasmicMembrane944
CYOE_ECOLIP0AEA5
cyoE
b0428 JW0418 integral IM protein (SEC in IM), Protoheme IX farnesyltransferase CyoEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN580.11TMH02.59686-99Ni12-30o36-60i80-102o108-127i134-156o162-183i209-227o233-252i264-285oCytoplasmicMembrane10
CYSW_ECOLIP0AEB0
cysW
b2423 JW2416 integral IM protein (SEC in IM), Sulfate transport system permease CysW Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.154TMH013.7791-66Ni20-43o63-89i101-124o144-162i202-228o248-268iCytoplasmicMembrane1052
DACA_ECOLIP0AEB2
dacA

pfv

pfvb0632 JW0627 periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacAPeripheral inner membrane protein facing the periplasmAnchored to inner membrane by C-terminal tailPMID: 2194801. Using natural isotope abundance NMR, the structure of the PBP 5 anchor peptide within a micelle was determinedPMID: 20192190. The structure conforms to a helix-bend-helix-turn-helix motif and reveals that the anchor enters the membrane so as to form an amphiphilic structure within the interface of the hydrophilic/hydrophobic boundary regions near the lipid head groups. PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones PMID: 20545860. PMID: 3279397 PMID: 8424800 DacA(PBP-5) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. A pH dependent loss of alpha-helical conformation in the peptide corresponding to the PBP5 C-terminus was found to correlate with the susceptibility of PBP5 to membrane extraction. PMID: 9371419PMID: 2194801 PMID: 20192190 PMID: 20545860 PMID: 24057063 PMID: 3279397 PMID: 8424800 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell inner membrane; Peripheral membrane protein; Cytoplasmic side Membrane anchored (Experimental)SECyesyesY300.737SpI2917.6151-00Yn13-24c29/30oCytoplasmicMembrane98
YMFR_ECOLIP75979
ymfR
b1150 JW1136 integral IM protein (SEC in IM), YmfRIntegral Inner MembraneBBinoutin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN220.153TMH011.0494-21Yn4-17c21/22o31-49iCytoplasmicMembrane916
YCGJ_ECOLIP76001
ycgJ
b1177 JW1166 periplasmic protein (SEC through IM), YcgJPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY230.404SpI228.58297-00Yn6-16c24/25oUnknown277
YNAI_ECOLIP0AEB5
ynaI
b1330 JW1323 integral IM protein (SEC in IM), Low conductance mechanosensitive channel YnaI Integral Inner Membrane PMID: 22874652 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN220.127TMH07.65863-45No12-30i42-65o77-96i117-134o140-159iCytoplasmicMembrane1014
YOAE_ECOLIP0AEC0
yoaE
b1816 JW1805 integral IM protein (SEC in IM), UPF0053 YoaEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.115TMH01.99313-77No12-36i48-69o81-101i122-148o154-172i184-207o213-231iCytoplasmicMembrane1010
ARCB_ECOLIP0AEC3
arcB
b3210 JW5536 integral IM protein (SEC in IM), Aerobic respiration control sensor ArcBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN360.18TMH09.51619-22No20-46i58-77oCytoplasmicMembrane10
BARA_ECOLIP0AEC5
barA
b2786 JW2757 integral IM protein (SEC-YidC in IM), Signal transduction histidine-kinase BarAIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCnoyesN340.123TMH07.64299-22No12-31i177-199oCytoplasmicMembrane1056
YMGE_ECOLIP76011
ymgE

tag

tagb1195 JW1184 integral IM protein (SEC in IM), UPF0410 YmgEIntegral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN270.106TMH011.3901-33No6-21i28-47o59-81iCytoplasmicMembrane1016
DCUS_ECOLIP0AEC8
dcuS

yjdH

yjdHb4125 JW4086 integral IM protein (SEC-YidC in IM), Sensor histidine kinase DcuSIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCnoyesN480.105TMH05.80308-22Ni21-41o183-205iCytoplasmicMembrane1035
DCRB_ECOLIP0AEE1
dcrB

yhhR

yhhRb3472 JW5682 IM lipoprotein (SEC through IM, inserted in OM by LOL), DcrBInner Membrane LipoproteinPMID: 12558182 PMID: 15911532Inner Membrane LipoproteinESoluble IM FractionPeriplasmInner membrane lipoproteinSECLOLyesyesN300.169SpII1921.1Pos+2=D00Yn8-15c20/21oPeriplasmic10100
DEGS_ECOLIP0AEE3
degS

hhoB htrH

hhoB htrHb3235 JW3204 periplasmic protein (SEC through IM), Serine endoprotease DegS Periplasmic Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428PMID: 11442831 PMID: 17360428PeriplasmicGSoluble IM FractionCell inner membrane Membrane anchoredSECyesyesN280.167SpI284.49468-10Yn9-20c28/29oPeriplasmic975
DGAL_ECOLIP0AEE5
mglB

GBP

GBPb2150 JW2137 periplasmic protein (SEC through IM), D-galactose-binding periplasmic MglBPeriplasmic Potential Skp substrate PMID: 19953543 PMID: 7012152 PMID: 9298646PMID: 19953543 PMID: 7012152 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSKPnoyesY240.861SpI2323.7963-10Yn5-16c23/24oPeriplasmic1075
YDBL_ECOLIP76076
ydbL
b1383 JW5216 periplasmic protein (SEC through IM), YdbLPeriplasmicGN/APeriplasmicSECyesyesY220.838SpI2115.5115-10Yn4-15c21/22oUnknown251
YNBA_ECOLIP76090
ynbA
b1408 JW1405 integral IM protein (SEC in IM), YnbAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN500.126TMH0-0.193337-55No31-64i85-107o113-132i152-168o174-191iCytoplasmicMembrane1011
YDCZ_ECOLIP76111
ydcZ
b1447 JW1442 integral IM protein (SEC in IM), YdcZIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN610.165TMH08.79151-54Yn4-19c24/25o34-55i67-88o94-114i126-145oCytoplasmicMembrane1014
DPPB_ECOLIP0AEF8
dppB
b3543 JW3512 integral IM protein (SEC in IM), Dipeptide transport system permease DppBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.168TMH06.13051-66Ni9-30o100-123i135-160o201-221i259-285o305-331iCytoplasmicMembrane10
DPPC_ECOLIP0AEG1
dppC
b3542 JW3511 integral IM protein (SEC-YidC in IM), Dipeptide transport system permease DppCIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN590.13TMH00.391748-55No32-53i102-127o147-173i215-244o264-287iCytoplasmicMembrane104
DSBA_ECOLIP0AEG4
dsbA

dsf ppfA

dsf ppfAb3860 JW3832 periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbA PeriplasmicPeriplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544PMID: 18406599 PMID: 20615876 PMID: 19525348 PMID: 13129941 PMID: 15838024 PMID: 9298646 PMID: 9300489 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SRP (exp)SECyesyesY200.75SpI1916.7987-00Yn4-15c19/20oPeriplasmic1071
DSBC_ECOLIP0AEG6
dsbC

xprA

xprAb2893 JW2861 periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbC PeriplasmicPeriplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544PMID: 18406599 PMID: 20615876 PMID: 8168498 PMID: 9298646 PMID: 10700276 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY210.778SpI2021.5074-00Yn4-15c20/21oPeriplasmic1082
YNCH_ECOLIP76118
yncH
b1455 JW5235 periplasmic protein (SEC through IM), YncHPeriplasmicGIM (predicted)CytoplasmicSECnoyesN400.113CYT0-0.200913-00Yn2-13c18/19oUnknown235
SAFA_ECOLIP76136
safA

yneN

yneNb1500 JW1494.1 integral IM protein (SEC in IM), Two-component-system connector SafA Integral Inner Membrane PMID: 17998538Integral Inner MembraneBBinout/inIM (predicted)Cell inner membrane -SRPSECnoyesN400.109CYT0-0.200913-11No19-40iCytoplasmicMembrane9
YNEE_ECOLIP76146
yneE
b1520 JW5245 integral IM protein (SEC in IM), UPF0187 YneEIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN380.113TMH013.5444-34Ni28-46o52-70i208-229o235-255iCytoplasmicMembrane9
RZPQ_ECOLIP76158
rzpQ

ydfP

ydfPb1553 JW1545 periplasmic protein (SEC through IM), RzpQPeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoN230.245SpI220.491707-10Yn7-17c22/23oUnknown219
RSEP_ECOLIP0AEH1
rseP

ecfE yaeL

ecfE yaeLb0176 JW0171 integral IM protein (SEC in IM), Regulator of sigma-E protease RseP Integral Inner Membrane Fluorescent fusions of RseP have been monitored via fluorescence microscopy. RseP has been found to generally associated with the membrane.PMID: 17272300 PMID: 11689431 PMID: 11750129 PMID: 11867724 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN330.109TMH03.00034-44No6-29i98-119o376-396i426-444oCytoplasmicMembrane1016
ELAB_ECOLIP0AEH5
elaB

yfbD

yfbDb2266 JW2261 integral IM protein (SEC in IM), ElaBIntegral Inner Membrane ElaB is an YqjD paralog and also has transmembrane motif at the C-terminal region. ElaB is an YqjD paralog and also has transmembrane motif at the C-terminal region. Probably binds to the membrane and ribosomes in a manner similar to that of YqjD PMID: 22661687.PMID: 22661687Integral Inner MembraneBBoutout/inIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN330.125CYT0-0.200913-11No81-99iUnknown299
EMRB_ECOLIP0AEJ0
emrB
b2686 JW2661 integral IM protein (SEC-YidC in IM), Multidrug export EmrB Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 15919996PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSEC-YidCyesyesN360.208TMH05.07213-1313Ni12-33o53-72i81-100o106-128i140-161o167-189i201-220o232-251i272-291o303-324i336-353o365-390i480-498oCytoplasmicMembrane10
ENVZ_ECOLIP0AEJ4
envZ

ompB perA tpo

ompB perA tpob3404 JW3367 integral IM protein (SEC in IM), Osmolarity sensor EnvZ Integral Inner Membrane Contains HAMP an intrinsically disordered domain. EnvZ HAMP domain by itself is unable to form a stable structure and that this structural fragility may be important for its role in signal transduction. PMID: 2668953 PMID: 15522865 PMID: 17635923 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.263TMH07.11037-22Ni12-39o160-179iCytoplasmicMembrane10
YNFA_ECOLIP76169
ynfA
b1582 JW1574 integral IM protein (SEC in IM), UPF0060 YnfAIntegral Inner Membrane Dual topology membrane protein: can insert into the membrane in both directions. PMID: 15919996PMID: 11867724 PMID: 15919996 PMID: 24688744 PMID: 16429150Integral Inner MembraneBBpolyout/out, in/inIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN620.224TMH010.6101-44No6-26i33-54o60-78i85-105oCytoplasmicMembrane10
YNFD_ECOLIP76172
ynfD
b1586 JW5259 periplasmic protein (SEC through IM), YnfDPeriplasmicExperimentally verified gene-product. PMID: 9868784PMID: 9868784PeriplasmicGSoluble IM FractionPeriplasmic (Experimental)SECnoyesY220.874SpI2122.7125-00Yn3-14c21/22oExtracellular979
YDGK_ECOLIP76180
ydgK
b1626 JW1618 integral IM protein (SEC in IM), YdgKIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.147TMH019.3259-44Ni12-36o56-78i90-108o114-133iCytoplasmicMembrane10
FDNI_ECOLIP0AEK7
fdnI
b1476 JW1472 integral IM protein (SEC in IM), Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunit FdnIIntegral Inner Membrane PMID: 15342602 PMID: 11266632 PMID: 11884747 PMID: 15919996 PMID: 11929547Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN370.156TMH022.1464-44Ni21-43o55-76i117-138o150-174iCytoplasmicMembrane1035
FDOI_ECOLIP0AEL0
fdoI
b3892 JW3863 integral IM protein (SEC in IM), Formate dehydrogenase, cytochrome b556(fdo) subunit FdoIIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.115TMH016.4965-44Ni20-43o55-73i113-133o145-172iCytoplasmicMembrane1016
FEPB_ECOLIP0AEL6
fepB
b0592 JW0584 periplasmic protein (SEC through IM), Ferrienterobactin-binding periplasmic protein FepBPeriplasmicPMID: 10986237PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.581SpI2613.3736-00Yn10-21c26/27oPeriplasmic1030
FLGM_ECOLIP0AEM4
flgM
b1071 JW1058 Flagellar cytoplasmic & extra-cellular protein (Flagellum secreted), Negative regulator of flagellin synthesis FlgMCytoplasmic, Extra-cellularAnti-sigma28 factor. FlgM is an anti-sigma factor that binds and inactivates sigma28 (FliA_Ecoli). Once the hook and basal body is assembled, FlgM is secreted by the flagellar export system thereby freeing sigma28 to activate transcription.PMID: 10322161 PMID: 12631703 PMID: 9988479Cytoplasmic, Extra-cellularA, XN/ACytoplasmicFlagellum FlayesyesN490.115CYT0-0.200913-00NiCytoplasmic989
FLIY_ECOLIP0AEM9
fliY

yzzR

yzzRb1920 JW1905 periplasmic protein (SEC through IM), L-cystine-binding FliY PeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesN300.555SpI2910.7798-00Yn10-21c29/30oPeriplasmic1096
YDIY_ECOLIP76206
ydiY
b1722 JW1711 periplasmic protein (SEC through IM), YdiYPeriplasmicGN/APeriplasmicSECyesyesY260.481SpI2520.5374-00Yn7-18c23/24oUnknown215
YNIB_ECOLIP76208
yniB
b1726 JW1715 integral IM protein (SEC in IM), YniBIntegral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell membrane Integral Membrane ProteinSRPSECyesyesN440.107TMH05.0431-34No15-33i45-63o83-101i150-172oCytoplasmicMembrane1040
YNJA_ECOLIP76222
ynjA
b1753 JW1742 integral IM protein (SEC in IM), YnjAIntegral Inner MembraneBBinout/inIM (predicted)CytoplasmicSRPSECyesyesN260.102CYT0-0.200913-01No33-51iCytoplasmicMembrane941
FTSL_ECOLIP0AEN4
ftsL

mraR yabD

mraR yabDb0083 JW0081 IM lipoprotein (SEC through IM), Cell division FtsLInner Membrane LipoproteinLocalizes to potential division sites in filamentous cells. Proven pignal peptidase II substrate. PMID: 1332942PMID: 15174130 PMID: 21890895 PMID: 1332942 PMID: 10027987 PMID: 11703663 PMID: 11948172 PMID: 15165235Inner Membrane LipoproteinEIM (predicted)Cell inner membrane Membrane anchored (Experimental)SECyesyesN240.167CYT0-0.200913-11No35-57iCytoplasmic825
GALP_ECOLIP0AEP1
galP
b2943 JW2910 integral IM protein (SEC in IM), Galactose-proton symporter GalPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN260.158TMH014.2152-1212Ni15-44o56-77i84-103o109-130i142-160o172-192i251-274o290-310i322-342o348-373i385-406o418-436iCytoplasmicMembrane10
GLNH_ECOLIP0AEQ3
glnH
b0811 JW0796 periplasmic protein (SEC through IM), Glutamine-binding periplasmic GlnHPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350PMID: 18498364 PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECSURAyesyesY230.851SpI2219.9168-00Yn7-18c22/23oPeriplasmic1062
GLNP_ECOLIP0AEQ6
glnP
b0810 JW0795 integral IM protein (SEC in IM), Glutamine transport system permease GlnP Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.119TMH07.24978-35No23-42i54-78o90-113i125-144o190-209iCytoplasmicMembrane10
GLPF_ECOLIP0AER0
glpF
b3927 JW3898 integral IM protein (SEC in IM), Glycerol uptake facilitator GlpFIntegral Inner Membrane PMID: 11266632 PMID: 11039922 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN310.125TMH09.6263-66Ni12-34o46-79i86-108o145-166i178-202o230-251iCytoplasmicMembrane10
YNJF_ECOLIP76226
ynjF
b1758 JW1747 integral IM protein (SEC in IM), YnjFIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN430.114CYT0-0.200913-55No33-60i81-102o114-135i147-167o173-195iCytoplasmicMembrane10
YNJH_ECOLIP76227
ynjH
b1760 JW1749 periplasmic protein (SEC through IM), YnjHPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECnoyesY190.569SpI1811.8884-00Yn4-14c18/19oCytoplasmicMembrane927
GLTJ_ECOLIP0AER3
gltJ
b0654 JW0649 integral IM protein (SEC in IM), Glutamate/aspartate import permease GltJIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN400.108TMH09.38551-55No24-50i71-88o108-127i179-199o211-233iCytoplasmicMembrane10
GLTK_ECOLIP0AER5
gltK
b0653 JW0648 integral IM protein (SEC in IM), Glutamate/aspartate import permease GltKIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN310.129TMH07.84867-55No20-44i65-83o95-112i153-178o198-219iCytoplasmicMembrane10
GLTS_ECOLIP0AER8
gltS

gltC

gltCb3653 JW3628 integral IM protein (SEC in IM), Sodium/glutamate symporter GltSIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN270.145CYT0-0.200913-911Yn7-18c26/27o36-52i72-90o96-119i126-148o160-184i215-239o245-268i275-297o303-323i335-357o369-394iCytoplasmicMembrane10
HDEA_ECOLIP0AES9
hdeA

yhhC yhiB

yhhC yhiBb3510 JW3478 periplasmic protein (SEC through IM), Acid stress chaperone HdeAPeriplasmicExperimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 10623550 PMID: 17085547 PMID: 20080625 PMID: 8455549 PMID: 25403562 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECYfgMyesyesY220.698SpI2116.385-10Yn4-15c21/22oUnknown2108
HDEB_ECOLIP0AET2
hdeB

yhhD yhiC

yhhD yhiCb3509 JW5669 periplasmic protein (SEC through IM), Acid stress chaperone HdeBPeriplasmicExperimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 8455550 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 17085547 PMID: 8455550 PMID: 25403562 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECYfgMyesyesY300.837SpI2916.8932-00Yn10-21c29/30oUnknown263
YOAI_ECOLIP76239
yoaI
b1788 JW5885 integral IM protein (SEC in IM), YoaIIntegral Inner MembraneBBinoutout/inIM (predicted)Membrane -SRPSECnoyesN210.164CYT0-0.200913-11No6-30iCytoplasmicMembrane9
YECT_ECOLIP76296
yecT
b1877 JW5310 periplasmic protein (SEC through IM), YecTPeriplasmicGN/APeriplasmicSECnoyesY190.831SpI1811.1311-00Yn4-14c18/19oUnknown431
FLHE_ECOLIP76297
flhE
b1878 JW1867 periplasmic protein (SEC through IM), FlhEPeriplasmicPart of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECFlayesyesY170.511SpI168.24229-00Yn3-14c26/27oUnknown268
HDED_ECOLIP0AET5
hdeD

yhiA

yhiAb3511 JW3479 integral IM protein (SEC in IM), HdeDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.139TMH03.36767-66Ni23-42o48-69i76-97o103-124i136-155o161-187iCytoplasmicMembrane10
HISJ_ECOLIP0AEU0
hisJ
b2309 JW2306 periplasmic protein (SEC through IM), Histidine-binding periplasmic HisJPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 19953543 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY230.792SpI2220.7158-10Yn4-14c22/23oPeriplasmic10
HISM_ECOLIP0AEU3
hisM
b2307 JW2304 integral IM protein (SEC in IM), Histidine transport system permease HisM Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN420.123TMH010.1822-43No20-45i57-79o200-221iCytoplasmicMembrane10
SKP_ECOLIP0AEU7
skp

hlpA ompH

hlpA ompHb0178 JW0173 periplasmic protein (SEC through IM), Chaperone SkpPeriplasmicFolding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamA. PMID: 2167239 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350PMID: 21370981 PMID: 19525348 PMID: 23641708 PMID: 2318304 PMID: 1838129 PMID: 8730870 PMID: 2167239 PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasm Periplasmic(experimental)SECyesyesY210.881SpI2022.0906-00Yn4-15c20/21oPeriplasmic1048
HYFE_ECOLIP0AEW1
hyfE
b2485 JW2470 integral IM protein (SEC in IM), Hydrogenase-4 component E HyfEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN540.165TMH04.55769-77No6-24i31-54o60-80i92-114o126-143i152-171o177-196iCytoplasmicMembrane10
YECR_ECOLIP76308
yecR
b1904 JW1892 OM lipoprotein (SEC through IM, inserted in OM by LOL), YecROuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesN250.325SpII158.98675Pos+2=T00Yn3-14c22/23oUnknown215
YEDR_ECOLIP76334
yedR
b1963 JW1946 integral IM protein (SEC in IM), YedRIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN230.118TMH00.40092-23No6-22i43-62o86-109iCytoplasmicMembrane1043
YOEA_ECOLIP76356
yoeA
b4582 JW1980 b1995 b1998OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative YoeAOuter Membrane b-barrel proteinHN/AOuter Membrane ??-barrel proteinSECBAMpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoY290.402SpI288.15876-00Yn12-23c28/29oOuterMembrane9
KGTP_ECOLIP0AEX3
kgtP

witA

witAb2587 JW2571 integral IM protein (SEC in IM), Alpha-ketoglutarate permease KgtPIntegral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECnoyesN90.105TMH02.8422-1212Ni21-42o62-84i96-116o122-139i160-183o195-214i244-266o278-299i311-331o337-360i372-393o405-423iCytoplasmicMembrane10
LIVH_ECOLIP0AEX7
livH
b3457 JW3422 integral IM protein (SEC in IM), High-affinity branched-chain amino acid transport system permease LivH Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN450.186TMH016.6878-88No20-39i46-64o70-89i105-122o158-175i204-230o242-270i282-301oCytoplasmicMembrane10
MALE_ECOLIP0AEX9
malE
b4034 JW3994 periplasmic & peripheral IM protein (SEC through IM), Maltose/maltodextrin-binding periplasmic MalEPeripheral inner membrane protein facing the periplasm, periplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. MalE is maintained in an unfolded conformation by the cytoplasmic chaperone SecB. Potential Skp substrate PMID: 19953543 PMID: 9298646 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 18498364 PMID: 1989077 PMID: 19953543 PMID: 9298646 PMID: 3009404 PMID: 25403562Peripheral inner membrane protein facing the periplasm, periplasmicF2, GN/APeriplasm Periplasmic (Experimental)SecBSECSURA,SKP,YfgMyesyesY270.712SpI2617.3972-00Yn9-17c26/27oPeriplasmic1096
MARC_ECOLIP0AEY1
marC

ydeB ydeC

ydeB ydeCb1529 JW1522 integral IM protein (SEC in IM), UPF0056 MarCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN380.16TMH010.7793-55No12-32i44-62o68-89i151-172o192-214iCytoplasmicMembrane105
YEGJ_ECOLIP76394
yegJ
b2071 JW2056 periplasmic protein (SEC through IM), YegJPeriplasmicGN/APeriplasmicSECnoyesY190.264SpI199.75379-00Yn4-14c19/20oUnknown279
YEGR_ECOLIP76406
yegR
b2085 JW5837 OM lipoprotein (SEC through IM, inserted in OM by LOL), YegROuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLyesyesY220.281SpII1715.1742Pos+2=A00Yn4-14c22/23oUnknown254
YFAT_ECOLIP76466
yfaT
b2229 JW2223 periplasmic protein (SEC through IM), YfaTPeriplasmicGN/APeriplasmicSECnoyesY200.277SpI193.99575-00Yn4-15c19/20oCytoplasmicMembrane926
MDFA_ECOLIP0AEY8
mdfA

cmlA cmr

cmlA cmrb0842 JW0826 integral IM protein (SEC-YidC in IM), Multidrug transporter MdfA Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN320.122TMH06.20433-1212Ni15-33o53-72i84-103o109-130i142-164o170-188i221-246o258-275i287-308o314-335i347-367o379-399iCytoplasmicMembrane10
MLTD_ECOLIP0AEZ7
mltD

dniR yafG

dniR yafGb0211 JW5018 OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase D MltDOuter Membrane LipoproteinProven signal peptidase II substrate. PMID: 15174133PMID: 15174130 PMID: 15174133Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN270.22SpII1518.5563Pos+2=Q00Yn5-13c18/19oUnknown229
MODB_ECOLIP0AF01
modB

chlJ

chlJb0764 JW0747 integral IM protein (SEC in IM), Molybdenum transport system permease ModB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN340.137TMH08.18777-55No20-37i49-70o90-115i136-158o198-220iCytoplasmicMembrane1020
MOTB_ECOLIP0AF06
motB
b1889 JW1878 integral IM protein (SEC in IM), Motility B MotBIntegral Inner Membrane MotB has one membrane-spanning alpha-helix but the majority of the molecule is in the periplasmic space with a peptidoglycan-binding domain near its C-terminus PMID: 2447650PMID: 19364805 PMID: 23193105 PMID: 2447650 PMID: 12730325Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane Membrane anchored (Experimental)SRPSECyesyesN320.155CYT0-0.200913-11No28-47iCytoplasmicMembrane733
YFDX_ECOLIP76520
yfdX
b2375 JW2372 periplasmic protein (SEC through IM), YfdXPeriplasmicPMID: 11157960PeriplasmicGN/APeriplasmic (Experimental)SECyesyesY220.881SpI2120.5809-00Yn4-15c21/22oUnknown249
YFDY_ECOLIP76521
yfdY
b2377 JW2374 integral IM protein (SEC in IM), YfdYIntegral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN280.123TMH019.5928-33No6-25i32-49o55-75iCytoplasmicMembrane1058
YFEZ_ECOLIP76538
yfeZ
b2433 JW2426 integral IM protein (SEC in IM), YfeZIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.118TMH01.95358-44Ni21-41o61-78i90-110o116-135iCytoplasmicMembrane1040
YFFQ_ECOLIP76548
yffQ
b2448 periplasmic protein (SEC through IM), YffQPeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoN190.652SpI183.21713-00Yn3-13c18/19oUnknown4
MURJ_ECOLIP0AF16
murJ

mviN yceN

mviN yceNb1069 JW1056 integral IM protein (SEC in IM), Lipid II flippase MurJIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN220.165SpI230.241812-1313No27-45i82-110o130-154i161-182o188-205i236-262o274-292i313-334o354-371i383-402o408-429i441-466o478-501iCytoplasmicMembrane10
NARV_ECOLIP0AF32
narV
b1465 JW1460 integral IM protein (SEC in IM), Respiratory nitrate reductase 2 gamma chain NarVIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN190.114TMH010.9916-55No12-29i50-69o89-111i132-151o180-199iCytoplasmicMembrane10
YJBB_ECOLIP0AF43
yjbB
b4020 JW3980 integral IM protein (SEC in IM), YjbBIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN240.13TMH01.33234-88Yn2-13c17/18o45-64i76-98o104-121i133-151o171-194i206-226o238-262i274-294oCytoplasmicMembrane104
GHXP_ECOLIP0AF52
ghxP

yjcD

yjcDb4064 JW4025 integral IM protein (SEC in IM), Guanine/hypoxanthine permease GhxP Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN590.114CYT0-0.200913-1313Ni29-49o55-74i81-101o107-129i141-165o171-195i202-220o256-280i301-321o333-350i357-378o390-416i428-448oCytoplasmicMembrane10
YJCO_ECOLIP0AF56
yjcO
b4078 JW4039 periplasmic protein (SEC through IM), Sel1-repeat-containing YjcO Periplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY180.752SpI1713.9637-00Yn4-13c17/18oUnknown282
YFGI_ECOLIP76573
yfgI
b2506 JW2490 periplasmic protein (SEC through IM), YfgIPeriplasmicGN/APeriplasmicSECnoyesY200.625SpI197.20342-00Yn4-14c32/33oUnknown211
YQIJ_ECOLIP76657
yqiJ
b3050 JW3022 integral IM protein (SEC in IM), YqiJIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN390.125TMH03.14983-33Ni12-35o66-86i98-117oCytoplasmicMembrane1012
YZGL_ECOLIP76692
yzgL
b3427 JW3390 integral IM protein (SEC in IM), YzgLIntegral Inner MembraneBBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECpseudogene (Uniprot)nonoN230.127TMH017.4708-22Ni12-40o46-69iCytoplasmicMembrane1029
YJEI_ECOLIP0AF70
yjeI
b4144 JW5736 OM lipoprotein (SEC through IM, inserted in OM by LOL), YjeIOuter Membrane Lipoprotein Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130PMID: 15174130 PMID: 12488465 PMID: 20932056Outer Membrane LipoproteinISoluble IM FractionOuter membrane Lipoprotein (Experimental)SECLOLyesyesY280.443SpII1820.5638Pos+2=S00Yn6-17c22/23oUnknown287
LPTF_ECOLIP0AF98
lptF

yjgP

yjgPb4261 JW4218 integral IM protein (SEC in IM), polysaccharide export system permease LptF Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN380.124CYT0-0.200913-66Ni16-39o59-76i97-119o268-289i296-316o328-348iCytoplasmicMembrane1013
NARX_ECOLIP0AFA2
narX

narR

narRb1222 JW1213 integral IM protein (SEC in IM), Nitrate/nitrite sensor NarX Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN470.224SpI334.68578-22Ni12-36o154-174iCytoplasmicMembrane1044
NFRB_ECOLIP0AFA5
nfrB
b0569 JW0558 integral IM protein (SEC in IM), Bacteriophage adsorption B NfrBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN200.134CYT0-0.200913-44No12-42i365-384o390-410i422-443oCytoplasmicMembrane107
HOKE_ECOLIP77091
hokE

ybdY

ybdYb4415 JW5084 integral IM protein (SEC in IM), Toxic HokE Integral Inner Membrane Potentially toxic protein.PMID: 19121005Integral Inner MembraneBBinoutin/outN/ACell inner membrane Membrane anchoredSRPSECnoyesN200.132SpI242.84692-10Yn6-24c29/30oPeriplasmic951
YKFB_ECOLIP77162
ykfB
b0250 JW0239 periplasmic protein (SEC through IM), YkfBPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoY240.716SpI2317.8556-00Yn11-19c23/24oUnknown259
YKGH_ECOLIP77180
ykgH
b0310 JW0302 integral IM protein (SEC in IM), YkgHIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN450.104CYT0-0.200913-22Ni21-42o186-209iUnknown225
LOMR_ECOLIP77184
lomR
b4570 JW5884/JW5904 b1369/b1371OM b-barrel protein (SEC through IM), Putative LomROuter Membrane b-barrel proteinHN/ACytoplasmicSECInsertion Sequence, pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN270.169SpI260.733275-00NiOuterMembrane10
NHAB_ECOLIP0AFA7
nhaB
b1186 JW1175 integral IM protein (SEC in IM), Na(+)/H(+) antiporter NhaB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN440.149TMH010.0118-1212No20-47i59-77o89-108i120-141o147-165i202-219o239-260i299-331o351-368i389-413o453-471i478-498oCytoplasmicMembrane10
NIKC_ECOLIP0AFA9
nikC
b3478 JW3443 integral IM protein (SEC in IM), Nickel transport system permease NikC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN250.156TMH010.244-66Ni12-32o75-100i121-148o177-196i203-222o242-260iCytoplasmicMembrane10
NLPI_ECOLIP0AFB1
nlpI

yhbM

yhbMb3163 JW3132 OM lipoprotein (SEC through IM, inserted in OM by LOL), NlpIOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130 Induction of NlpI inhibits nucleoid division and halts cell growth. Elevation of NlpI expression enhances the transcription and the outer membrane localization of the heat shock protein IbpA and IbpB. NlpI physically interacts with IbpB. The C-terminus of NlpI is critical for the enhancement in IbpA and IbpB production, and the N-terminus of NlpI is required for the outer membrane localization of NlpI, IbpA, and IbpB. PMID: 25699035PMID: 15174130 PMID: 25699035Outer Membrane LipoproteinIN/ACell membrane; Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN260.209SpII1814.4676Pos+2=S00Yn8-19c31/32oCytoplasmicMembrane1056
NUOA_ECOLIP0AFC3
nuoA
b2288 JW2283 integral IM protein (SEC in IM), NADH-quinone oxidoreductase subunit A NuoAIntegral Inner Membrane Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 15919996 PMID: 21541338Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN440.182TMH012.5237-33No12-37i68-88o100-122iCytoplasmicMembrane1048
CUSF_ECOLIP77214
cusF

cusX ylcC

cusX ylcCb0573 JW0562 periplasmic protein (SEC through IM), Cation efflux system CusFPeriplasmicPMID: 12813074PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.444SpI228.68332-00Yn4-15c22/23oPeriplasmic10
YAHC_ECOLIP77219
yahC
b0317 JW0309 integral IM protein (SEC in IM), YahCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN700.119TMH010.9451-45Yn4-15c23/24o33-49i61-80o86-104i109-127o133-152iCytoplasmicMembrane10
ESSQ_ECOLIP77237
essQ

ydfS

ydfSb1556 JW5255 integral IM protein (SEC in IM), Prophage lysis S homolog EssQ Integral Inner MembraneBBinoutin/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)yesnoN380.166CYT0-0.200913-21Yn7-15c20/21o36-58iUnknown217
YAFU_ECOLIP77354
yafU
b0218 JW0207 integral IM protein (SEC in IM), Putative YafUIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECpseudogene (Uniprot)nonoN390.16TMH01.65262-22Ni21-38o44-64iCytoplasmicMembrane1073
NUOH_ECOLIP0AFD4
nuoH
b2282 JW2277 integral IM protein (SEC in IM), NADH-quinone oxidoreductase subunit H NuoHIntegral Inner Membrane PMID: 20732298 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN330.139TMH07.32865-89Ni12-32o52-69i81-100o112-133i154-174o186-207i234-257o269-289i301-322oCytoplasmicMembrane10
NUOJ_ECOLIP0AFE0
nuoJ
b2280 JW2275 integral IM protein (SEC in IM), NADH-quinone oxidoreductase subunit J NuoJIntegral Inner Membrane PMID: 20732298 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.504TMH010.2272-54Yn3-14c18/19o28-49i56-74o94-112i133-158oCytoplasmicMembrane1082
NUOK_ECOLIP0AFE4
nuoK
b2279 JW2274 integral IM protein (SEC in IM), NADH-quinone oxidoreductase subunit K NuoKIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 20732298 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.166TMH08.16488-33No6-24i29-54o60-81iCytoplasmicMembrane10
NUOM_ECOLIP0AFE8
nuoM
b2277 JW2272 integral IM protein (SEC in IM), NADH-quinone oxidoreductase subunit M NuoMIntegral Inner Membrane PMID: 20732298 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN590.116TMH06.38779-1414No6-23i30-48o77-103i115-134o140-159i180-199o219-244i256-274o286-307i314-333o345-363i383-405o417-440i461-481oCytoplasmicMembrane10
NUON_ECOLIP0AFF0
nuoN
b2276 JW2271 integral IM protein (SEC in IM), NADH-quinone oxidoreductase subunit N NuoNIntegral Inner Membrane PMID: 20732298 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN610.262TMH09.81822-1414No6-28i35-55o75-93i105-122o128-147i159-181o201-226i238-259o271-290i297-316o328-351i371-394o406-426i447-470oCytoplasmicMembrane10
YAFY_ECOLIP77365
yafY
b0251 JW0240 IM lipoprotein (SEC through IM, inserted in OM by LOL), YafYInner Membrane LipoproteinPMID: 23138451Inner Membrane LipoproteinEN/ACell inner membrane CytoplasmicSECLOLProphage Integrase (EcoGene)nonoY210.302SpII2017.0831Pos+2=D00Yn6-17c21/22oUnknown227
HOKB_ECOLIP77494
hokB

ydcB

ydcBb4428 JW5225 integral IM protein (SEC in IM), Toxic HokB Integral Inner Membrane Potentially toxic protein.PMID: 19121005Integral Inner MembraneBBinout/inN/ACell inner membrane Membrane anchoredSRPSECnoyesN260.105CYT0-0.200913-11No6-24iPeriplasmic942
YBDJ_ECOLIP77506
ybdJ
b0580 JW0569 integral IM protein (SEC in IM), YbdJIntegral Inner MembraneBBinoutin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN360.165TMH013.1416-21Yn7-25c33/34o49-70iCytoplasmicMembrane9
NUPC_ECOLIP0AFF2
nupC

cru

crub2393 JW2389 integral IM protein (SEC in IM), Nucleoside permease NupC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN270.126TMH00.463943-89No6-23i30-48o87-108i167-187o193-212i242-271o277-298i341-361o381-399iCytoplasmicMembrane10
NUPG_ECOLIP0AFF4
nupG
b2964 JW2932 integral IM protein (SEC in IM), Nucleoside permease NupGIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN510.127TMH010.0497-1212Ni12-31o37-58i70-89o95-116i137-155o161-179i211-235o255-275i282-300o306-327i348-369o381-402iCytoplasmicMembrane10
OPPB_ECOLIP0AFH2
oppB
b1244 JW1236 integral IM protein (SEC in IM), Oligopeptide transport system permease OppB Integral Inner Membrane Opp-LacZ hybrid proteins were used to show that OppB, OppC and OppD are membrane-associated proteins. PMID: 2821267PMID: 15919996 PMID: 2821267Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN480.124TMH03.13181-66Ni12-30o100-121i133-161o173-192i228-254o274-300iCytoplasmicMembrane10
OPPC_ECOLIP0AFH6
oppC
b1245 JW1237 integral IM protein (SEC in IM), Oligopeptide transport system permease OppC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN640.213TMH00.755301-66Ni40-64o103-128i140-158o164-183i218-247o267-290iCytoplasmicMembrane10
YDDL_ECOLIP77519
yddL
b1472 JW1468 OM b-barrel protein (SEC through IM), YddLOuter Membrane b-barrel proteinHN/APeriplasmicSECpseudogene (Ochman & Davalos, 2006), pseudogene (Uniprot)yesyesY220.898SpI2118.2436-00Yn5-16c21/22oOuterMembrane948
YAGL_ECOLIP77607
yagL
b0278 JW0272 integral IM protein (SEC in IM), YagLIntegral Inner MembraneBBoutin/outIM (predicted)CytoplasmicSRPSECProphage Integrase (EcoGene)nonoN320.112CYT0-0.200913-01Ni12-32oCytoplasmic813
YAHH_ECOLIQ2EEQ3
yahH
b0322 JW5893 integral IM protein (SEC in IM), Putative YahHMembrane anchored by EchoLOCATIONIntegral Inner MembraneB-N/AMembrane anchoredSECpseudogene (Uniprot), pseudogene (Ecogene)nonoN420.264CYT0-0.200913-00NiUnknown2
YNID_ECOLIQ2EES1
yniD
b4535 JW5911 integral IM protein (SEC in IM), YniDIntegral Inner Membrane Small membrane protein detected experimentally.PMID: 19121005Integral Inner MembraneBBinoutin/outIM (predicted)Membrane Membrane anchoredSRPSECnoyesN310.1TMH04.22082-10Yn14-26c30/31oCytoplasmicMembrane9
OSMY_ECOLIP0AFH8
osmY
b4376 JW4338 periplasmic & extra-cellular protein (SEC through IM), Osmotically-inducible Y OsmYPeriplasmic, Extra-cellular The most efficient excreting fusion partner, OsmY, was used as a carrier protein to excrete heterologous proteins into the medium. PMID: 18727129 The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane PMID: 12175925PMID: 1317380 PMID: 9298646 PMID: 18727129 PMID: 12878000Periplasmic, Extra-cellularG, XN/APeriplasmPeriplasmic (Experimental)SECyesyesY290.787SpI2823.4143-00Yn12-23c28/29oPeriplasmic10108
PBP7_ECOLIP0AFI5
pbpG

yohB

yohBb2134 JW5355 periplasmic protein (SEC through IM), D-alanyl-D-alanine endopeptidase PbpGPeriplasmicPMID: 7721700PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY260.58SpI2517.1021-00Yn6-17c25/26oPeriplasmic1082
PERM_ECOLIP0AFI9
perM

yfgO

yfgOb2493 JW2478 integral IM protein (SEC in IM), Putative permease PerM Integral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN410.135TMH020.7793-77No20-51i72-93o151-177i217-236o242-270i282-301o307-332iCytoplasmicMembrane1026
PITA_ECOLIP0AFJ7
pitA

pit

pitb3493 JW3460 integral IM protein (SEC in IM), Low-affinity inorganic phosphate transporter 1 PitAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN390.147TMH08.59981-109No12-32i52-76o96-116i123-143o155-177i208-227o233-255i379-402o474-494iCytoplasmicMembrane10
YOHO_ECOLIQ2EES6
yohO
b4542 JW5354 integral IM protein (SEC in IM), UPF0387 YohOIntegral Inner Membrane Small membrane protein detected experimentally PMID: 19121005Integral Inner MembraneBBinoutout/inN/ACell inner membrane Membrane anchoredSRPSECnoyesN260.149TMH04.51604-11No6-31iCytoplasmicMembrane9
YPFN_ECOLIQ2EET2
ypfN
b4547 JW2457 integral IM protein (SEC in IM), UPF0370 YpfNIntegral Inner MembraneBBinout/inIM (predicted)Cell membrane Membrane anchoredSRPSECnoyesN260.107CYT0-0.200913-11No6-24iUnknown271
YTJA_ECOLIQ2M5U1
ytjA
b4568 JW5891 integral IM protein (SEC in IM), UPF0391 YtjAIntegral Inner MembraneBBinoutin/inN/ACell membrane Integral Membrane ProteinSRPSECnoyesN310.166TMH011.6664-21Yn4-14c19/20o29-49iCytoplasmicMembrane9
YSAB_ECOLIQ2M7M3
ysaB
b4553 JW3532 OM lipoprotein (SEC through IM, inserted in OM by LOL), YsaBOuter Membrane LipoproteinIN/ACell membrane Outer membrane LipoproteinSECLOLyesyesY270.418SpII1715.6662Pos+2=S00Yn5-16c26/27oUnknown236
PNUC_ECOLIP0AFK2
pnuC
b0751 JW0734 integral IM protein (SEC in IM), Nicotinamide riboside transporter PnuC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN200.115TMH03.57186-67No22-40i47-65o71-88i109-139o159-178i185-205o211-226iCytoplasmicMembrane10
POTB_ECOLIP0AFK4
potB
b1125 JW1111 integral IM protein (SEC in IM), Spermidine/putrescine transport system permease PotB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.107TMH05.71787-67No6-26i54-78o90-108i120-138o144-163i184-214o242-262iCytoplasmicMembrane10
POTC_ECOLIP0AFK6
potC
b1124 JW1110 integral IM protein (SEC in IM), Spermidine/putrescine transport system permease PotC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN600.118TMH010.8409-66Ni12-30o64-86i98-122o128-151i172-195o230-252iCytoplasmicMembrane10
POTD_ECOLIP0AFK9
potD
b1123 JW1109 periplasmic protein (SEC through IM), Spermidine/putrescine-binding periplasmic PotDPeriplasmic Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 1939142 PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY240.865SpI2325.0348-00Yn8-18c23/24oPeriplasmic10
POTI_ECOLIP0AFL1
potI
b0857 JW0841 integral IM protein (SEC in IM), Putrescine transport system permease PotI Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN260.124TMH014.5424-67Ni12-35o60-90i102-129o141-163i184-206o212-232i244-264oCytoplasmicMembrane10
YICS_ECOLIQ2M7X4
yicS
b4555 JW5965 periplasmic protein (SEC through IM), YicSPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesN220.681SpI2110.9898-00Yn4-14c21/22oUnknown2
YOBH_ECOLIQ2MB16
yobH
b4536 JW5298 integral IM protein (SEC in IM), YobHSignal peptide predicted, single transmembrane helice predicted which would be removed upon signal peptide cleavage. Putative outer membrane or exported. Taxonomy: Klebsiella pneumoniae.Integral Inner MembraneBBoutin/outN/AMembrane anchoredSRPSECnoyesN340.169TMH05.54741-10Yn10-20c24/25oCytoplasmicMembrane957
SIRB2_ECOLIQ46755
ychQ

sirB2

sirB2b1213 JW1204 integral IM protein (SEC in IM), YchQIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN390.138TMH06.48818-44No6-28i40-61o73-93i105-123oCytoplasmicMembrane1025
PPIA_ECOLIP0AFL3
ppiA

rot rotA

rot rotAb3363 JW3326 periplasmic protein (SEC through IM), Peptidyl-prolyl cis-trans isomerase A PpiAPeriplasmic Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 2190212 PMID: 22030350PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY250.753SpI2424.1414-00Yn4-15c24/25oPeriplasmic1073
PQIA_ECOLIP0AFL9
pqiA

pqi5A

pqi5Ab0950 JW0933 integral IM protein (SEC in IM), Intertransport PqiA Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN330.119CYT0-0.200913-88Ni53-76o96-122i142-160o166-185i257-277o304-329i350-371o377-400iCytoplasmicMembrane10
PROX_ECOLIP0AFM2
proX

proU

proUb2679 JW2654 periplasmic protein (SEC through IM), Glycine betaine/proline betaine-binding periplasmic ProXPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Identified DsbA substrate PMID: 19953543 PMID: 3305496 Disulfide bridge (157 163) Disulfide bond-containing protein PMID: 25664544PMID: 18498364 PMID: 19953543 PMID: 3305496 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECSURA,DSBAyesyesY220.863SpI2116.9579-00Yn5-16c21/22oPeriplasmic1052
PSPB_ECOLIP0AFM9
pspB
b1305 JW1298 integral IM protein (SEC in IM), Phage shock B PspBIntegral Inner Membrane Defects in yidC and sec secretion induce psp operon. PMID: 14594846PMID: 14594846Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane; Single-pass membrane proteinMembrane anchoredSRPSECProphage Transposase (by homology to BL21)noyesN300.112CYT0-0.200913-11No6-24iCytoplasmicMembrane1062
YADH_ECOLIP0AFN6
yadH
b0128 JW0124 integral IM protein (SEC in IM), transport permease YadHIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.101TMH08.75539-67Yn4-9c14/15o24-46i58-84o104-132i139-164o170-191i203-221o227-248iCytoplasmicMembrane104
PBL_ECOLIQ46790
pbl
b2854 periplasmic protein (SEC through IM), Putative PG-binding-like protein PblPeriplasmicGN/APeriplasm CytoplasmicSECpseudogene (Uniprot)nonoY250.548SpI242.39101-00Yn8-19c24/25oPeriplasmic928
YGFX_ECOLIQ46824
ygfX

cptA

cptAb2896 JW2864 integral IM protein (SEC in IM), YgfXIntegral Inner Membrane PMID: 22239607Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECyesyesN390.141TMH05.61025-12Ni12-32o38-54iCytoplasmicMembrane933
YQGA_ECOLIQ46831
yqgA
b2966 JW2934 integral IM protein (SEC in IM), YqgAIntegral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN200.108TMH03.98827-68No6-23i30-50o56-75i103-123o135-157i164-181o187-206i213-233oCytoplasmicMembrane10
YADS_ECOLIP0AFP0
yadS
b0157 JW0153 integral IM protein (SEC in IM), UPF0126 YadSIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.24TMH02.68818-77No6-23i30-50o62-79i91-110o116-137i149-168o174-190iCytoplasmicMembrane10
YBHR_ECOLIP0AFP9
ybhR
b0792 JW5803 integral IM protein (SEC in IM), Probable multidrug ABC transporter permease YbhR Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN410.153CYT0-0.200913-66Ni21-41o173-197i218-243o255-277i284-302o345-363iCytoplasmicMembrane10
YBHS_ECOLIP0AFQ2
ybhS
b0793 JW0777 integral IM protein (SEC in IM), Probable multidrug ABC transporter permease YbhS Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN670.102CYT0-0.200913-66Ni29-47o182-205i232-255o261-283i295-314o346-370iCytoplasmicMembrane107
DAUA_ECOLIP0AFR2
dauA

ychM

ychMb1206 JW5189 integral IM protein (SEC in IM), C4-dicarboxylic acid transporter DauAIntegral Inner Membrane PMID: 23278959 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN690.11CYT0-0.200913-119No33-53i60-80o100-118i130-152o172-198i210-231o278-300i358-388o408-438iCytoplasmicMembrane10
YCJO_ECOLIP0AFR7
ycjO
b1311 JW1304 integral IM protein (SEC in IM), ABC transporter permease YcjOIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.147TMH012.2234-65Yn14-25c30/31o77-98i110-130o163-189i210-233o265-283iCytoplasmicMembrane10
YGHG_ECOLIQ46835
yghG
b2971 JW2938 OM lipoprotein (SEC through IM, inserted in OM by LOL), YghGOuter Membrane LipoproteinBelongs to the GSP C family. Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLnoyesY290.253SpII2417.6171Pos+2=A00Yn10-22c27/28oUnknown240
YGIZ_ECOLIQ46867
ygiZ
b3027 JW2995 integral IM protein (SEC in IM), YgiZIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.138TMH04.05338-33Ni12-29o54-72i84-103oCytoplasmicMembrane1027
YQEJ_ECOLIQ46943
yqeJ
b2848 JW5455 integral IM protein (SEC in IM), YqeJIntegral Inner MembraneBBoutin/outN/AMembrane PeriplasmicSRPSECnoyesN280.216SpI276.43067-11Ni7-27oUnknown2
YDCV_ECOLIP0AFR9
ydcV
b1443 JW1438 integral IM protein (SEC in IM), ABC transporter permease YdcVIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN330.134TMH08.81303-66Ni12-33o71-91i103-127o133-153i189-212o232-256iCytoplasmicMembrane10
LSRD_ECOLIP0AFS1
lsrD

ydeZ

ydeZb1515 JW1508 integral IM protein (SEC in IM), Autoinducer 2 import system permease LsrDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN240.162TMH09.62635-99Yn9-17c23/24o33-56i63-82o88-110i117-140o160-181i212-234o240-260i267-286o292-310iCytoplasmicMembrane10
TQSA_ECOLIP0AFS5
tqsA

ydgG

ydgGb1601 JW1593 integral IM protein (SEC in IM), AI-2 transport TqsAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.372TMH08.36419-88Ni12-31o37-55i62-83o144-165i197-218o224-246i258-279o299-325iCytoplasmicMembrane10
YDIK_ECOLIP0AFS7
ydiK
b1688 JW1678 integral IM protein (SEC in IM), Putative transport YdiK Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN420.172TMH013.7653-99Ni12-31o37-53i65-86o160-179i191-208o214-235i247-271o277-295i307-334oCytoplasmicMembrane10
MEPM_ECOLIP0AFS9
mepM

yebA

yebAb1856 JW5304 integral IM protein (SEC in IM), Murein DD-endopeptidase MepM MepMIntegral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane CytoplasmicSRPSECyesyesN120.112CYT0-0.200913-11Ni21-39oUnknown233
YFEK_ECOLIQ47702
yfeK
b2419 JW2412 periplasmic protein (SEC through IM), YfeKPeriplasmicGN/APeriplasmicSECnoyesY200.758SpI192.85333-00Yn4-15c19/20oUnknown277
YQJK_ECOLIQ47710
yqjK
b3100 JW3071 integral IM protein (SEC in IM), YqjKIntegral Inner MembraneBBinoutin/outIM (predicted), Detected in IM FractionMembrane anchoredSRPSECyesyesN620.14CYT0-0.200913-11Ni47-65oUnknown230
YJDP_ECOLIQ6BEX5
yjdP
b4487 JW5890 periplasmic protein (SEC through IM), YjdPPeriplasmicGN/APeriplasmicSECnoyesY230.721SpI2214.9551-00Yn6-17c22/23oUnknown2
LDRD_ECOLIQ6BF25
ldrD
b4453 JW5966 integral IM protein (SEC in IM), Small toxic polypeptide LdrDIntegral Inner Membrane Potentially toxic protein.PMID: 19121005Integral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane CytoplasmicSRPSECyesyesN210.155CYT0-0.200913-11No14-31iCytoplasmicMembrane9
YECS_ECOLIP0AFT2
yecS
b1918 JW1903 integral IM protein (SEC in IM), L-cystine transport system permease YecS Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN700.103TMH08.62641-33No21-45i57-78o186-206iCytoplasmicMembrane104
YEJB_ECOLIP0AFU0
yejB
b2178 JW2166 integral IM protein (SEC in IM), ABC transporter permease YejBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN190.157TMH04.74055-66Ni9-30o135-156i168-194o224-247i282-309o329-355iCytoplasmicMembrane10
YFBS_ECOLIP0AFU2
yfbS
b2292 JW2289 integral IM protein (SEC in IM), transporter YfbSIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN210.196TMH07.38351-1010Ni5-22o28-45i57-83o95-124i136-155o175-195i470-493o513-538i550-568o588-608iCytoplasmicMembrane10
YIBH_ECOLIP0AFV0
yibH
b3597 JW3571 integral IM protein (SEC in IM), YibHIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinMembrane anchoredSRPSECyesyesN530.113TMH02.25888-21Yn3-13c18/19o28-49iCytoplasmicMembrane1023
YHID_ECOLIP0AFV2
yhiD

yhhE

yhhEb3508 JW5670 integral IM protein (SEC in IM), Putative magnesium transporter YhiD Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN550.254CYT0-0.200913-55No6-22i34-54o69-87i92-112o118-138iCytoplasmicMembrane1028
LDRB_ECOLIQ6BF87
ldrB
b4421 JW5958 integral IM protein (SEC in IM), Small toxic polypeptide LdrBIntegral Inner Membrane Potentially toxic protein.PMID: 19121005Integral Inner MembraneBBinoutout/inIM (predicted)Cell inner membrane CytoplasmicSRPSECyesyesN210.121CYT0-0.200913-11No14-31iCytoplasmicMembrane9
YMGG_ECOLIQ7DFV3
ymgG
b1172 JW5178 integral IM protein (SEC in IM), UPF0757 YmgGIntegral Inner Membrane Belongs to the UPF0757 family. PMID: 18192383 PMID: 17787032Integral Inner MembraneBBpolyin/inN/AMembrane anchoredSRPSECnoyesY230.871SpI2223.9513-20Yn5-16c22/23oUnknown298
MEPS_ECOLIP0AFV4
mepS

spr yeiV

spr yeiVb2175 JW2163 OM lipoprotein (SEC through IM), inserted in OM by LOL), Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase MepSOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY340.288SpII2611.4182Pos+2=S00Yn14-24c29/30oUnknown236
RSEA_ECOLIP0AFX7
rseA

mclA yfiJ

mclA yfiJb2572 JW2556 integral IM protein (SEC in IM), Anti-sigma-E factor RseAIntegral Inner Membrane Responce to stress complex RpoE-RseA-RseB. Overproduction of N-terminal domain of RseA alone was shown to inhibit ??E activity. RseC which is an inner membrane protein, positively modulates ??E activity. The periplasmic domain of RseA interacts with RseB while its cytoplasmic domain interacts with RpoE, thereby sequestering RpoE away from the transcription complex. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428 The N-terminal domain of RseA sequesters ??E in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. PMID: 11777003 Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase. PMID: 12718891PMID: 9159523 PMID: 9159522 PMID: 12183369 PMID: 17360428 PMID: 11777003 PMID: 12718891Integral Inner MembraneB-IM (predicted-detected)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN620.226CYT0-0.200913-00NiUnknown259
RSEB_ECOLIP0AFX9
rseB
b2571 JW2555 periplasmic protein (SEC through IM), Sigma-E factor regulatory RseB PeriplasmicResponce to stress complex RpoE-RseA-RseB. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428 The N-terminal domain of RseA sequesters ??E in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. PMID: 11777003 PMID: 11777003PMID: 9159523 PMID: 9159522 PMID: 17360428 PMID: 11777003PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY240.61SpI2310.5992-00Yn4-16c28/29oPeriplasmic915
SANA_ECOLIP0AFY2
sanA

yeiF

yeiFb2144 JW2132 integral IM protein (SEC in IM), SanAIntegral Inner Membrane Related to vancomycin resistance and when overexpressed, is able to complement the permeability defects of a vancomycin-susceptible mutant.PMID: 8550448Integral Inner MembraneBBoutin/outN/ACell inner membrane; Single-pass membrane proteinPeriplasmicSRPSECyesyesN230.14TMH06.95693-10Yn5-18c22/23oCytoplasmicMembrane1010
SBMA_ECOLIP0AFY6
sbmA
b0377 JW0368 integral IM protein (SEC in IM), Peptide antibiotic transporter SbmA Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN300.182TMH018.7145-88Ni12-30o58-78i85-112o140-164i206-227o239-263i309-326o332-353iCytoplasmicMembrane10
TRKH_ECOLIP0AFZ7
trkH
b3849 JW5576 integral IM protein (SEC in IM), Trk system potassium uptake TrkH Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006)yesyesN410.127TMH06.71344-1011Ni12-32o38-57i69-90o136-163i184-204o241-262i274-294o331-363i396-417o423-444i456-481oCytoplasmicMembrane10
WZZE_ECOLIP0AG00
wzzE

wzz yifC

wzz yifCb3785 JW5601 integral IM protein (SEC in IM), ECA polysaccharide chain length modulation protein WzzEIntegral Inner Membrane PMID: 15919996 PMID: 16816184 PMID: 16199561Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN470.253TMH08.70367-22No31-50i323-342oCytoplasmicMembrane1019
SOHB_ECOLIP0AG14
sohB
b1272 JW1264 integral IM protein (SEC in IM), Probable protease SohB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionCell inner membrane; Single-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.141CYT0-0.200913-21No12-32iCytoplasmicMembrane108
YIBN_ECOLIP0AG27
yibN
b3611 JW3586 integral IM protein (SEC in IM), YibNIntegral Inner Membrane PMID: 15919657Integral Inner MembraneBBinout/inIM (predicted), Detected in IM FractionMembrane anchoredSRPSECyesyesN360.109CYT0-0.200913-11No12-30iUnknown248
RHTB_ECOLIP0AG34
rhtB

yigK

yigKb3824 JW5585 integral IM protein (SEC in IM), Homoserine/homoserine lactone efflux protein RhtBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane Protein (Experimental)SRPSECpseudogene (Ochman & Davalos, 2006)noyesN250.247TMH011.1132-54Yn7-18c23/24o39-65i116-138o144-168i188-205oCytoplasmicMembrane10
RHTC_ECOLIP0AG38
rhtC

yigJ

yigJb3823 JW5586 integral IM protein (SEC in IM), Threonine efflux protein RhtCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006)yesyesN250.144TMH08.74493-66No6-26i38-63o69-87i119-140o152-174i186-204oCytoplasmicMembrane10
RMUC_ECOLIP0AG71
rmuC

yigN

yigNb3832 JW3809 integral IM protein (SEC in IM), DNA recombination RmuCIntegral Inner Membrane Based on the fact that RmuC is a DNA recombination enzyme we conluded that its N-terminus lays in the periplasm.PMID: 16011798Integral Inner MembraneBBinout/inN/ACytoplasmicSRPSECyesyesN290.536SpI281.08238-11No6-25iUnknown215
SUBI_ECOLIP0AG78
sbp
b3917 JW3888 periplasmic protein (SEC through IM), Sulfate-binding SbpPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9298646PMID: 18498364 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSURAyesyesY200.802SpI1915.2757-00Yn4-14c19/20oPeriplasmic1070
UGPB_ECOLIP0AG80
ugpB
b3453 JW3418 periplasmic protein (SEC through IM), sn-glycerol-3-phosphate-binding periplasmic UgpB Periplasmic Identified DsbA substrate PMID: 19953543 PMID: 3062310 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 3062310 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.804SpI2317.1867-00Yn7-18c23/24oPeriplasmic1064
PSTS_ECOLIP0AG82
pstS

phoS

phoSb3728 JW3706 periplasmic protein (SEC through IM), Phosphate-binding PstS PeriplasmicPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Copurifies with trigger factor in pull down assays. PMID: 9298646PMID: 18498364 PMID: 19737520 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TFSECSURAyesyesY260.546SpI2523.9055-10Yn6-17c25/26oPeriplasmic1079
SECD_ECOLIP0AG90
secD
b0408 JW0398 integral IM protein (SEC in IM), translocase subunit SecD Integral Inner Membrane PMID: 16079137 PMID: 15919657 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN250.158TMH01.57163-66Ni7-25o453-472i477-497o503-524i545-571o577-605iCytoplasmicMembrane1036
SECF_ECOLIP0AG93
secF
b0409 JW0399 integral IM protein (SEC in IM), translocase subunit SecF Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN30.112TMH02.24012-66Ni24-43o142-163i170-191o197-217i248-266o272-297iCytoplasmicMembrane10
SECE_ECOLIP0AG96
secE

prlG

prlGb3981 JW3944 integral IM protein (SEC in IM), translocase subunit SecE Integral Inner Membrane Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN480.137TMH07.80526-33No16-36i43-61o95-119iCytoplasmicMembrane107
SECG_ECOLIP0AG99
secG
b3175 JW3142 integral IM protein (SEC in IM), Protein-export SecGIntegral Inner Membrane PMID: 15919657 PMID: 15919996Integral Inner MembraneBBinoutin/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.153TMH011.5638-21Yn4-22c27/28o51-70iCytoplasmicMembrane1049
SECY_ECOLIP0AGA2
secY

prlA

prlAb3300 JW3262 integral IM protein (SEC in IM), translocase subunit SecY Integral Inner Membrane PMID: 15919996 PMID: 3004955Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN470.104CYT0-0.200913-1010Ni23-44o75-98i119-139o154-174i186-203o215-237i274-296o316-337i373-391o397-418iCytoplasmicMembrane10
UHPT_ECOLIP0AGC0
uhpT
b3666 JW3641 integral IM protein (SEC in IM), Hexose-6-phosphate:phosphate antiporter UhpTIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN530.105TMH00.582302-1212Ni28-45o65-85i97-117o123-148i160-183o189-210i260-278o298-318i327-345o351-380i392-411o423-446iCytoplasmicMembrane10
SLT_ECOLIP0AGC3
slt

sltY

sltYb4392 JW4355 periplasmic protein (SEC through IM), Soluble lytic murein transglycosylase SltPeriplasmic Disulfide bridge (133 166) Disulfide bond-containing protein PMID: 25664544PMID: 1938883 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY280.803SpI2712.0915-00Yn11-22c27/28oPeriplasmic1010
MLTF_ECOLIP0AGC5
mltF

yfhD

yfhDb2558 JW2542 periplasmic peripheral OM protein (SEC through IM), Membrane-bound lytic murein transglycosylase F MltFPeripheral outer membrane protein facing the periplasmPMID: 18234673Peripheral outer membrane protein facing the periplasmF3N/ACell outer membrane PeriplasmicSECnoyesN330.227SpI303.07095-10Yn9-20c32/33oCytoplasmic910
SMP_ECOLIP0AGC7
ytjB

smp

smpb4387 JW4350 integral IM protein (SEC in IM), YtjBIntegral Inner Membrane Has an aminoterminal stop transfer or signal sequence hydrophobic region and a second hydrophobic region near the C-terminus that may function as a stop transfer sequence; The protein was shown to fractionate with the bacterial membrane. No processing of tPMID: 2684780Integral Inner MembraneBBinoutin/outIM (predicted)Cell inner membrane Membrane anchoredSRPSECpseudogene (Ochman & Davalos, 2006)nonoN300.128SpI307.66045-11Yn13-24c29/30o164-184iCytoplasmicMembrane1063
SODC_ECOLIP0AGD1
sodC
b1646 JW1638 periplasmic protein (SEC through IM), Superoxide dismutase SodCPeriplasmic Disulfide bridge (74 169) PMID: 9405149 Disulfide bond-containing protein PMID: 25664544PMID: 8626323 PMID: 9405149 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.82SpI1916.2903-00Yn4-14c19/20oPeriplasmic10
SODF_ECOLIP0AGD3
sodB
b1656 JW1648 periplasmic protein (SEC through IM), Superoxide dismutase SodBPeriplasmicThe superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are <i>E.coli</i> proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm.PeriplasmicGN/ACytoplasmicSECyesyesN170.122CYT0-0.200913-00NiPeriplasmic977
SSTT_ECOLIP0AGE4
sstT

ygjU

ygjUb3089 JW3060 integral IM protein (SEC in IM), Serine/threonine transporter SstT Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.148TMH07.50816-88Ni18-36o42-72i84-106o149-166i187-209o215-240i290-315o327-352iCytoplasmicMembrane1015
XYLE_ECOLIP0AGF4
xylE
b4031 JW3991 integral IM protein (SEC in IM), D-xylose-proton symporter XylEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN300.155TMH06.47202-1211Yn13-24c32/33o56-78i90-109o129-151i163-186o198-220i270-293o313-334i346-364o370-395i407-426o446-463iCytoplasmicMembrane10
YHHJ_ECOLIP0AGH1
yhhJ
b3485 JW5677 integral IM protein (SEC in IM), transport permease YhhJIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.231SpI401.57995-56Ni174-200o220-244i256-281o287-306i318-337o343-364iCytoplasmicMembrane106
SAPB_ECOLIP0AGH3
sapB
b1293 JW1286 integral IM protein (SEC in IM), Putrescine export system permease SapB Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN280.197TMH016.0324-56Ni9-29o82-101i113-141o175-193i245-263o283-305iCytoplasmicMembrane109
SAPC_ECOLIP0AGH5
sapC
b1292 JW1285 integral IM protein (SEC in IM), Putrescine export system permease SapC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN510.132TMH05.59827-56Ni31-54o99-123i144-169o189-208i220-241o261-283iCytoplasmicMembrane1010
PSTC_ECOLIP0AGH8
pstC

phoW

phoWb3727 JW3705 integral IM protein (SEC in IM), Phosphate transport system permease PstC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN30.109TMH04.7509-66Ni21-43o75-100i112-143o163-186i227-249o282-304iCytoplasmicMembrane10
RBSC_ECOLIP0AGI1
rbsC
b3750 JW3729 integral IM protein (SEC in IM), Ribose import permease RbsC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN580.121TMH06.39117-810Ni23-43o49-68i75-93o99-119i126-144o170-190i221-240o252-270i275-292o298-316iCytoplasmicMembrane1018
XYLH_ECOLIP0AGI4
xylH
b3568 JW3540 integral IM protein (SEC in IM), Xylose transport system permease XylH Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.13TMH04.89182-1011Ni25-45o65-83i90-107o113-132i139-156o176-196i217-235o241-259i290-306o326-357i369-385oCytoplasmicMembrane10
UBIA_ECOLIP0AGK1
ubiA

cyr

cyrb4040 JW4000 integral IM protein (SEC in IM), 4-hydroxybenzoate octaprenyltransferase UbiAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN360.118TMH06.00125-77Ni23-40o46-70i99-132o152-182i210-229o235-252i273-289oCytoplasmicMembrane10
YHHT_ECOLIP0AGM0
yhhT
b3474 JW5680 integral IM protein (SEC in IM), Putative transport YhhT Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN310.162TMH05.38885-88Ni12-31o37-55i62-87o133-163i197-215o221-246i253-275o295-315iCytoplasmicMembrane104
YICG_ECOLIP0AGM2
yicG
b3646 JW3621 integral IM protein (SEC in IM), UPF0126 YicGIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN220.368CYT0-0.200913-77No6-23i30-52o64-82i89-109o115-136i148-167o173-191iCytoplasmicMembrane10
URAA_ECOLIP0AGM7
uraA
b2497 JW2482 integral IM protein (SEC in IM), Uracil permease UraAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN470.107TMH03.93213-1213Ni20-38o44-60i67-83o89-108i120-147o159-176i183-202o225-250i262-281o301-319i331-354o366-384i391-409oCytoplasmicMembrane10
XANP_ECOLIP0AGM9
xanP

yicE

yicEb3654 JW3629 integral IM protein (SEC in IM), Xanthine permease XanP Integral Inner Membrane PMID: 16096267 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN660.123CYT0-0.200913-1111No36-58i70-89o128-146i158-181o193-212i219-240o260-280i350-372o378-396i408-427o439-458iCytoplasmicMembrane109
MLTC_ECOLIP0C066
mltC

yggZ

yggZb2963 JW5481 OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase C MltCOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY240.3SpII1614.8852Pos+2=S00Yn5-15c20/21oUnknown217
PROP_ECOLIP0C0L7
proP
b4111 JW4072 integral IM protein (SEC in IM), Proline/betaine transporter ProPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN410.105CYT0-0.200913-1212Ni41-59o65-86i98-116o122-146i167-186o198-217i257-278o298-318i327-346o352-376i388-411o417-438iCytoplasmicMembrane10
MSCS_ECOLIP0C0S1
mscS

yggB

yggBb2924 JW2891 integral IM protein (SEC in IM), Small-conductance mechanosensitive channel MscSIntegral Inner Membrane PMID: 11266632 PMID: 12446901 PMID: 16079137 PMID: 15919657 PMID: 15919996 PMID: 12767977 PMID: 12015316 PMID: 10202137Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN520.15TMH0-0.184901-33No24-45i65-85o97-125iCytoplasmicMembrane1042
MEPA_ECOLIP0C0T5
mepA
b2328 JW2325 periplasmic protein (SEC through IM), Penicillin-insensitive murein endopeptidase MepAPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2187143 Disulfide bridge (44 265;187 235;216 223) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 19953543 PMID: 2187143 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECDSBA,DSBCyesyesY200.847SpI1922.606-00Yn4-14c19/20oPeriplasmic1016
DEGP_ECOLIP0C0V0
degP

htrA ptd

htrA ptdb0161 JW0157 periplasmic protein (SEC through IM), serine endoprotease DegP PeriplasmicSecB substrate. Substrate of periplasmic disulfide oxidoreductases DsbA. HtrA was not detected by the immunoblotting technique in the periplasm or in the fraction of soluble proteins but was found in the inner membrane. DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into 12 and 24-mer cages with large chambers that house the substrate for degradation or refolding. Size exclusion chromatography and electron microscopy show that the size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme. Identified DsbA substrate PMID: 19953543 PMID: 2180903 Disulfide bridges between 83 and 95 PMID: 12878036 Disulfide bridge (83 95) PMID: 12878036 Disulfide bond-containing protein PMID: 25664544PMID: 16352602 PMID: 14726535 PMID: 21370981 PMID: 19525348 PMID: 9083020 PMID: 21526129 PMID: 19953543 PMID: 2180903 PMID: 12878036 PMID: 25664544PeriplasmicGN/ACell inner membrane Periplasmic (Experimental)SECBSECDSBAyesyesY270.841SpI2628.3128-00Yn4-19c26/27oPeriplasmic1031
EMTA_ECOLIP0C960
emtA

mltE sltZ ycgP

mltE sltZ ycgPb1193 JW5821 OM lipoprotein (SEC through IM), inserted in OM by LOL), Endo-type membrane-bound lytic murein transglycosylase A EmtAOuter Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY490.204SpII1518.8581Pos+2=S00Yn5-13c18/19oCytoplasmicMembrane915
EPTC_ECOLIP0CB39
eptC

cptA yijP

cptA yijPb3955 JW3927 integral IM protein (SEC in IM), Phosphoethanolamine transferase EptC Integral Inner Membrane PMID: 16079137Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN90.182TMH012.4414-55Ni20-39o45-63i70-91o120-139i151-169oCytoplasmicMembrane1026
UIDB_ECOLIP0CE44
uidB

gusB uidP

gusB uidPb1616 JW1608 integral IM protein (SEC in IM), Glucuronide carrier homolog UidBIntegral Inner Membrane PMID: 15919996 PMID: 15774881Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN610.197TMH05.96454-1212Ni12-35o41-60i81-101o113-136i148-170o182-201i230-257o263-284i296-316o322-343i376-393o405-427iCytoplasmicMembrane10
ACFD_ECOLIP0CK95
yghJ
b4466 JW5925 ECK2968IM lipoprotein (SEC through IM), inserted in OM by LOL), Putative AcfD homolog YghJInner Membrane LipoproteinEN/ACell membrane Inner membrane lipoproteinSECLOLnoyesY230.283SpII2337.591Pos+2=D00Yn10-21c26/27oUnknown6
RCSC_ECOLIP0DMC5
rcsC
b2218 JW5917/JW5920 integral IM protein (SEC in IM), Sensor histidine kinase RcsCIntegral Inner Membrane Fusions of rcsC to phoA that resulted in PhoA+ strains demonstrated that RcsC is a transmembrane protein with a periplasmic N-terminal domain and cytoplasmic C-terminal domain.PMID: 15919996 PMID: 2404948Integral Inner MembraneBBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN360.159TMH03.16217-21Yn20-31c40/41o314-334iCytoplasmicMembrane10
PHNE2_ECOLIP0DP70
phnE

phnE2

phnE2b4104 JW4065 b4103integral IM protein (SEC in IM), Putative cryptic phosphonate transport system permease PhnE2 Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006), pseudogene (Uniprot)noyesN360.143TMH08.60214-66Ni12-33o75-99i120-143o179-197i206-224o230-250iCytoplasmicMembrane107
RLPA_ECOLIP10100
rlpA
b0633 JW0628 OM lipoprotein (SEC through IM), inserted in OM by LOL), Endolytic PG transglycosylase RlpA RlpAOuter Membrane Lipoprotein Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005). Contains a SPOR domain. PMID: 15174130PMID: 15174130 PMID: 12488465 PMID: 15911532 PMID: 19880599 PMID: 20932056Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesN200.14SpII1719.3561Pos+2=T00Yn5-15c20/21oUnknown2
FADL_ECOLIP10384
fadL

ttr

ttrb2344 JW2341 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Long-chain fatty acid transport FadLOuter Membrane b-barrel proteinSubstrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 1987139PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 15178802 PMID: 16079137 PMID: 20932056 PMID: 19953543 PMID: 1987139Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECSURA,SKPBAMyesyesY260.796SpI2516.4528-00Yn10-20c25/26oOuterMembrane101
IAP_ECOLIP10423
iap
b2753 JW2723 periplasmic peripheral IM protein (SEC through IM), Alkaline phosphatase isozyme conversion IapPeripheral inner membrane protein facing the periplasmPMID: 3316184Peripheral inner membrane protein facing the periplasmF2N/AMembrane associated (Experimental)SECyesyesY250.707SpI2413.6553-00Yn9-20c24/25oUnknown425
NARK_ECOLIP10903
narK
b1223 JW1214 integral IM protein (SEC in IM), Nitrate/nitrite transporter NarK Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN600.17TMH00.305282-1212Ni38-62o74-92i104-124o130-158i178-197o217-234i255-278o290-308i320-339o351-377i404-425o437-457iCytoplasmicMembrane10
UGPA_ECOLIP10905
ugpA
b3452 JW3417 integral IM protein (SEC in IM), sn-glycerol-3-phosphate transport system permease UgpA Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN390.107TMH013.7961-66Ni12-31o78-100i112-130o157-181i206-228o267-288iCytoplasmicMembrane10
UGPE_ECOLIP10906
ugpE
b3451 JW3416 integral IM protein (SEC in IM), sn-glycerol-3-phosphate transport system permease UgpE Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN440.2TMH09.6685-67Ni7-31o81-105i112-133o145-166i187-210o216-238i250-272oCytoplasmicMembrane1028
NARI_ECOLIP11350
narI

chlI

chlIb1227 JW1218 integral IM protein (SEC in IM), Respiratory nitrate reductase 1 gamma chain NarIIntegral Inner Membrane Under anaerobic conditions in the presence of nitrate, E. coli synthesizes the cytoplasmic membrane-bound quinol-nitrate oxidoreductase (nitrate reductase A; NarGHI) PMID: 12910261. Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. PMID: 12910261 In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. PMID: 19295650 When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. PMID: 2674654 The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. PMID: 3371362 PMID: 3053688PMID: 11266632 PMID: 12910261 PMID: 15919996 PMID: 19295650 PMID: 2674654 PMID: 3371362 PMID: 3053688Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN190.113TMH011.8819-55No6-26i47-69o89-109i129-148o179-198iCytoplasmicMembrane10
FUCP_ECOLIP11551
fucP
b2801 JW2772 integral IM protein (SEC in IM), L-fucose-proton symporter FucPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN420.298TMH00.0692344-1212Ni21-41o61-85i92-111o117-134i155-177o210-228i262-282o294-315i327-345o351-373i385-405o411-431iCytoplasmicMembrane10
DAMX_ECOLIP11557
damX

yhfB

yhfBb3388 JW3351 integral IM protein (SEC in IM), Cell division DamXIntegral Inner Membrane Contain a SPOR Domain. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23290046 PMID: 19880599 PMID: 21541338Integral Inner MembraneBBinoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECnoyesN680.101CYT0-0.200913-11Ni103-124oUnknown2
ARGO_ECOLIP11667
argO

yggA

yggAb2923 JW2890 integral IM protein (SEC in IM), Arginine exporter ArgOIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein(experimental)SRPSECyesyesN250.273TMH06.65864-66No6-26i38-61o67-88i109-127o147-170i182-199oCytoplasmicMembrane1018
FECA_ECOLIP13036
fecA
b4291 JW4251 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Fe(3+) dicitrate transport FecAOuter Membrane b-barrel proteinSubstrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 2836368PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 11266632 PMID: 11872840 PMID: 20932056 PMID: 19953543 PMID: 2836368Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane ??-barrel protein (Experimental)SECSURA,SKPBAMnoyesY340.831SpI3318.814-00Yn19-29c33/34oOuterMembrane101
TREA_ECOLIP13482
treA

osmA

osmAb1197 JW1186 periplasmic protein (SEC through IM), trehalase TreAPeriplasmic Potential Skp substrate PMID: 19953543 PMID: 2671658 Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 2671658 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSKPyesyesY310.702SpI3017.5054-00Yn13-26c30/31oPeriplasmic1070
CSRD_ECOLIP13518
csrD

yhdA

yhdAb3252 JW3221 integral IM protein (SEC in IM), RNase E specificity factor CsrD Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN330.172TMH05.84825-22Ni7-31o130-155iCytoplasmicMembrane94
CHIA_ECOLIP13656
chiA

yheB

yheBb3338 JW3300 periplasmic & extra-cellular protein (SEC through IM), TSS secreted over OM), Probable bifunctional chitinase/lysozyme ChiAPeriplasmic, Extra-cellularCan be fully secreted when the cryptic T2S system is expressed. PMID: 10760150 Disulfide bridge (628 673) Disulfide bond-containing protein PMID: 25664544PMID: 11118204 PMID: 10760150 PMID: 25664544Periplasmic, Extra-cellularG, XN/APeriplasm Periplasmic (Experimental)SECT2SnoyesY250.672SpI2420.7505-00Yn11-20c24/25oPeriplasmic10
NHAA_ECOLIP13738
nhaA

ant

antb0019 JW0018 integral IM protein (SEC in IM), Na(+)/H(+) antiporter NhaA Integral Inner Membrane Structure of a Na+/H+ antiporter. A negatively charged ion funnel opens to the cytoplasm and ends in the middle of the membrane at the putative ion-binding site PMID: 15988517.PMID: 11266632 PMID: 15988517 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN340.227TMH07.93195-1111Ni12-39o59-79i91-114o126-145i154-175o181-200i207-238o258-276i288-312o324-351i363-380oCytoplasmicMembrane10
PROW_ECOLIP14176
proW
b2678 JW2653 integral IM protein (SEC-YidC in IM), Glycine betaine/proline betaine transport system permease ProW Integral Inner Membrane PMID: 10949305 PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN240.12CYT0-0.200913-66No97-118i125-143o149-171i192-219o271-291i303-320oCytoplasmicMembrane105
ZRAS_ECOLIP14377
zraS

hydH

hydHb4003 JW3967 integral IM protein (SEC in IM), Sensor ZraSIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN440.129TMH07.59471-22Ni12-35o202-222iCytoplasmicMembrane10
FECB_ECOLIP15028
fecB
b4290 JW4250 periplasmic & peripheral IM protein (SEC through IM), Fe(3+) dicitrate-binding periplasmic FecBPeripheral inner membrane protein facing the periplasm, periplasmicPMID: 2651410Peripheral inner membrane protein facing the periplasm, periplasmicF2, GN/APeriplasmPeriplasmic (Experimental)SECnoyesY220.843SpI218.42911-00Yn7-16c21/22oPeriplasmic98
FECD_ECOLIP15029
fecD
b4288 JW4248 integral IM protein (SEC in IM), Fe(3+) dicitrate transport system permease FecDIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN280.121TMH010.0321-77Yn4-19c31/32o55-76i85-103o109-126i138-158o226-256i268-290o296-315iCytoplasmicMembrane104
FECC_ECOLIP15030
fecC
b4289 JW4249 integral IM protein (SEC in IM), Fe(3+) dicitrate transport system permease FecCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.15TMH07.31223-89Yn8-27c36/37o60-81i93-111o117-140i152-171o177-194i201-220o240-270i282-304o310-328iCytoplasmicMembrane10
CSTA_ECOLIP15078
cstA

ybdC

ybdCb0598 JW0590 integral IM protein (SEC-YidC in IM), Peptide transporter CstA Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN210.185TMH08.48745-1816Ni7-26o32-52i93-111o117-138i159-185o191-209i221-240o260-277i284-305o325-345i373-393o470-494i515-534o546-571i578-598o648-671iCytoplasmicMembrane10
FLK_ECOLIP15286
flk

div

divb2321 JW2318 integral IM protein (SEC in IM), regulator Flk Integral Inner MembraneBBoutout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECyesyesN540.104CYT0-0.200913-11No313-330iCytoplasmicMembrane961
DHG_ECOLIP15877
gcd
b0124 JW0120 integral IM protein (SEC in IM), Quinoglucose dehydrogenase GcdIntegral Inner Membrane PMID: 16079137 PMID: 15919996 PMID: 8509415 PMID: 8419307 PMID: 8554505Integral Inner MembraneBBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN430.121TMH021.0845-55Ni12-35o41-58i65-81o87-106i113-140oCytoplasmicMembrane918
AROP_ECOLIP15993
aroP
b0112 JW0108 integral IM protein (SEC-YidC in IM), Aromatic amino acid transport AroPIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSEC-YidCyesyesN440.121TMH09.5384-1212Ni20-41o47-63i84-105o125-142i154-176o196-220i241-263o283-300i332-353o359-379i400-422o428-446iCytoplasmicMembrane10
PANF_ECOLIP16256
panF
b3258 JW3226 integral IM protein (SEC in IM), Sodium/pantothenate symporter PanFIntegral Inner Membrane Prediction of one very weak transmembrane region.PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN240.187TMH08.73514-1313No6-24i45-63o75-94i122-144o156-178i190-213o233-253i273-298o318-341i369-386o392-417i424-442o448-466iCytoplasmicMembrane10
HYCC_ECOLIP16429
hycC

hevC

hevCb2723 JW2693 integral IM protein (SEC in IM), Formate hydrogenlyase subunit 3 HycCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN320.168TMH011.9962-1416No6-26i33-58o78-96i108-124o130-146i153-173o197-218i230-251o257-280i292-312o332-349i370-395o415-435i456-480o500-521i587-605oCytoplasmicMembrane10
HYCD_ECOLIP16430
hycD

hevD

hevDb2722 JW2692 integral IM protein (SEC in IM), Formate hydrogenlyase subunit 4 HycDIntegral Inner Membrane PMID: 1625581 PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN300.198CYT0-0.200913-88No6-27i62-85o97-119i131-149o169-188i224-242o248-273i285-306oCytoplasmicMembrane10
PHND_ECOLIP16682
phnD

psiD

psiDb4105 JW4066 periplasmic protein (SEC through IM), Phosphonates-binding periplasmic protein PhnDPeriplasmicPMID: 16751609PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY270.567SpI2616.4228-00Yn6-21c26/27oPeriplasmic10
CYSP_ECOLIP16700
cysP
b2425 JW2418 periplasmic protein (SEC through IM), Thiosulfate-binding protein CysPPeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY260.741SpI2514.3932-00Yn10-20c25/26oPeriplasmic1061
CYST_ECOLIP16701
cysU

cysT

cysTb2424 JW2417 integral IM protein (SEC-YidC in IM), Sulfate transport system permease CysT Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN370.285TMH014.8684-75No12-37i58-84o104-125i137-156o242-263iCytoplasmicMembrane10117
FHUE_ECOLIP16869
fhuE
b1102 JW1088 OM b-barrel protein (SEC through IM, inserted in OM by BAM), FhuE receptor FhuEOuter Membrane b-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 3032906PMID: 10806384 PMID: 3032906Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane ??-barrel protein (Experimental)SECBAMnoyesY370.821SpI3620.6615-00Yn19-30c36/37oOuterMembrane100
MREC_ECOLIP16926
mreC
b3250 JW3219 integral IM protein (SEC in IM), Cell shape-determining MreCIntegral Inner Membrane PMID: 15612918Integral Inner MembraneBBoutin/outIM (predicted), Detected in IM FractionCell inner membrane Membrane anchoredSRPSECnoyesN250.114TMH02.1577-10Yn15-23c28/29oUnknown235
CIRA_ECOLIP17315
cirA

cir feuA

cir feuAb2155 JW2142 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Colicin I receptor CirAOuter Membrane b-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 20932056 PMID: 19953543 PMID: 10430870 PMID: 25664544Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane ??-barrel protein (Experimental)SECDSBA,SKPBAMyesyesY260.695SpI2511.8271-00Yn10-20c25/26oOuterMembrane1046
PTQC_ECOLIP17334
chbC

celB

celBb1737 JW1726 integral IM protein (SEC-YidC in IM), PTS system N,N'-diacetylchitobiose-specific EIIC component ChbCIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN280.124CYT0-0.200913-1010Ni35-55o86-107i114-133o145-167i187-207o235-258i301-320o332-352i359-384o404-429iCytoplasmicMembrane105
CYNX_ECOLIP17583
cynX
b0341 JW0332 integral IM protein (SEC-YidC in IM), Cyanate transport CynXIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCnoyesN550.147CYT0-0.200913-1211Yn2-10c18/19o34-55i67-85o91-113i125-147o153-173i201-224o230-251i263-281o287-309i321-339o354-372iCytoplasmicMembrane10
YFAA_ECOLIP17994
yfaA

pufY

pufYb2230 JW2224 periplasmic protein (SEC through IM), YfaAPeriplasmicGSoluble IM FractionPeriplasmicSECnoyesN310.233SpI305.42187-11Ni12-31oCytoplasmicMembrane989
PNCB_ECOLIP18133
pncB
b0931 JW0914 periplasmic protein (SEC through IM), Nicotinate phosphoribosyltransferase PncBPeriplasmicPncB is localized in the periplasm although it does not possess a signal peptide.PMID: 346557PeriplasmicGN/ACytoplasmicSECnoyesN610.11CYT0-0.200913-00NiCytoplasmic932
PGPA_ECOLIP18200
pgpA

yajN

yajNb0418 JW0408 integral IM protein (SEC in IM), Phosphatidylglycerophosphatase A PgpAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN450.137TMH013.1049-35Ni21-51o57-75i87-104o110-126i138-162oCytoplasmicMembrane1023
RSTB_ECOLIP18392
rstB

uspT

uspTb1609 JW1601 integral IM protein (SEC in IM), Sensor RstBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN320.112TMH02.20306-22No6-27i136-157oCytoplasmicMembrane1053
DMSB_ECOLIP18776
dmsB
b0895 JW0878 periplasmic peripheral IM protein (TAT via piggyback through IM), Anaerobic dimethyl sulfoxide reductase chain B DmsBPeripheral inner membrane protein facing the periplasmTAT (piggyback); DmsB is co-exported bound to Tat substrate DmsA. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332PMID: 3062312 PMID: 11389150 PMID: 9546395 PMID: 2170332Peripheral inner membrane protein facing the periplasmF2N/ACytoplasmicTAT (piggyback)yesyesN440.107CYT0-0.200913-00NiUnknown527
DMSA_ECOLIP18775
dmsA
b0894 JW5118 periplasmic peripheral IM protein (TAT-SEC through IM), Dimethyl sulfoxide reductase DmsAPeripheral inner membrane protein facing the periplasmTAT signal peptide, dmsABC complex. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD. PMID: 24093457 Fluorescence analysis of these YFP chimeras revealed a wide range of interactions such as the one between the Tat substrate dimethyl sulfoxide reductase (DmsA) and its dedicated proofreading chaperone DmsD. PMID: 20169075 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 PMID: 3280546 PMID: 3062312 Interacts with DmsD, a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 17218314 PMID: 3062312 PMID: 11389150 PMID: 24093457 PMID: 20169075 PMID: 9546395 PMID: 2170332 PMID: 3280546 PMID: 20153451Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane Periplasmic (Experimental)DmsDTAT,SECyesyesN460.537SpI455.24058-00NiCytoplasmicMembrane1022
DMSC_ECOLIP18777
dmsC
b0896 JW0879 integral IM protein (SEC in IM), Anaerobic dimethyl sulfoxide reductase chain C DmsCIntegral Inner Membrane DMSO reductase subunit C,Periplasmic. Has a membrane bound anchor. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 PMID: 15919996PMID: 15919996 PMID: 3062312 PMID: 11389150 PMID: 9546395 PMID: 2170332Integral Inner MembraneBBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.177TMH013.558-88No12-32i44-68o88-105i117-137o149-171i183-203o223-243i255-277oCytoplasmicMembrane10
GGT_ECOLIP18956
ggt
b3447 JW3412 periplasmic protein (SEC through IM), Glutathione hydrolase proenzyme GgtPeriplasmic Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 1360205 PMID: 2570061 PMID: 25403562PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECYfgMyesyesY260.723SpI2520.0548-00Yn10-21c25/26oPeriplasmic1036
PTOCB_ECOLIP19642
malX
b1621 JW1613 integral IM protein (SEC in IM), PTS system maltose-specific EIICB component MalXIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN440.116TMH07.8693-810Ni21-42o62-89i96-116o136-161i182-205o295-313i320-337o343-362i369-391o397-419iCytoplasmicMembrane105
AGP_ECOLIP19926
agp
b1002 JW0987 periplasmic protein (SEC through IM), Glucose-1-phosphatase AgpPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 2153660 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY230.88SpI2221.9324-00Yn5-16c22/23oPeriplasmic1062
TOLA_ECOLIP19934
tolA

cim excC lky

cim excC lkyb0739 JW0729 integral IM protein (SEC in IM), Tol-Pal system TolA Integral Inner Membrane The N-terminal coiled coil of YbgFvforms a stable trimer and controls the oligomeric status of YbgF. The stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein. PMID: 7744737 Disulfide bridge (363 388) Disulfide bond-containing protein PMID: 25664544PMID: 15919657 PMID: 20816983 PMID: 7744737 PMID: 25664544Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchored (Experimental)SRPSECyesyesN430.131CYT0-0.200913-11Ni12-31oUnknown250
PTFBC_ECOLIP20966
fruA

ptsF

ptsFb2167 JW2154 integral IM protein (SEC in IM), PTS system fructose-specific EIIB'BC component FruAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN200.169CYT0-0.200913-99Ni230-256o268-291i303-329o349-368i389-410o430-451i463-482o488-507i519-551oCytoplasmicMembrane107
SPEA_ECOLIP21170
speA
b2938 JW2905 periplasmic protein (SEC through IM), Biosynthetic arginine decarboxylase SpeAPeriplasmicTrigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction PMID: 19766000. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 2198270 Selective disruption of the cell envelope and an assessment of Spea activity or immunoprecipitable SpeA in various fractions demonstrated its location between the cytoplasmic membrane and peptidoglycan layer. PMID: 3894328PMID: 19737520 PMID: 15919657 PMID: 2198270 PMID: 19766000 PMID: 3894328PeriplasmicGSoluble IM FractionPeriplasm CytoplasmicTFSECyesyesN270.129CYT0-0.200913-00NiPeriplasmic1048
RNI_ECOLIP21338
rna

rnsA

rnsAb0611 JW0603 periplasmic protein (SEC through IM), Ribonuclease I RnaPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbA substrate PMID: 19953543 PMID: 2253883 Disulfide bridge (103 159) PMID: 14726535 PMID: 19953543 PMID: 2253883 PeriplasmicGN/APeriplasm Cytoplasm Periplasmic (Experimental)SECDSBA,DSBCyesyesY240.538SpI2515.6073-00Yn8-18c23/24oPeriplasmic1031
GLTP_ECOLIP21345
gltP
b4077 JW4038 integral IM protein (SEC in IM), Proton/glutamate-aspartate symporter GltPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN310.123TMH011.2813-1010Ni7-27o47-72i84-106o160-181i202-224o236-264i293-311o323-350i362-385o391-409iCytoplasmicMembrane1019
YCIC_ECOLIP21365
yciC
b1255 JW1247 integral IM protein (SEC in IM), UPF0259 YciCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN470.196TMH06.50025-66Ni20-41o77-104i116-136o142-166i187-212o218-239iCytoplasmicMembrane10
NMPC_ECOLIP21420
nmpC

phmA

phmAb0553 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative porin NmpC Outer Membrane b-barrel proteinPMID: 337109Outer Membrane b-barrel proteinHN/ACell outer membrane; Multi-pass membrane protein Outer Membrane ss-barrel protein(experimental)SECBAMpseudogene (Uniprot) yesnoY240.879SpI2325.1335-00Yn4-15c23/24oOuterMembrane1011
YCAD_ECOLIP21503
ycaD
b0898 JW0881 integral IM protein (SEC in IM), MFS-type transporter YcaD Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.154TMH014.4924-1211Yn10-18c23/24o47-65i74-92o98-119i131-149o161-181i202-223o235-253i265-283o289-311i323-345o351-369iCytoplasmicMembrane10
KDPD_ECOLIP21865
kdpD
b0695 JW0683 integral IM protein (SEC in IM), Sensor KdpDIntegral Inner Membrane PMID: 1532388 PMID: 21605551 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECnoyesN460.149CYT0-0.200913-44Ni399-419o425-443i448-465o477-498iCytoplasmicMembrane1022
YCBB_ECOLIP22525
ycbB
b0925 JW0908 periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase YcbBPeripheral outer membrane protein facing the periplasmYcbB forms direct meso- diaminopimelate (DAP-DAP, or 3-3) crosslinks within the peptidoglycan.PMID: 23832002Peripheral outer membrane protein facing the periplasmF3N/AMembrane PeriplasmicSECyesyesY310.448SpI3010.8337-00Yn11-22c30/31oPeriplasmic963
LIVM_ECOLIP22729
livM
b3456 JW3421 integral IM protein (SEC in IM), High-affinity branched-chain amino acid transport system permease LivM Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN210.39SpI2011.5293-1010Yn9-20c25/26o44-61i91-108o114-132i137-156o162-184i191-209o262-283i313-336o348-373i385-403oCytoplasmicMembrane10
TNAB_ECOLIP23173
tnaB

trpP

trpPb3709 JW5619/JW5622 integral IM protein (SEC in IM), Low affinity tryptophan permease TnaBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN250.14TMH016.6914-1111Ni12-32o38-61i82-105o125-143i155-174o194-219i226-246o282-310i322-341o347-369i381-406oCytoplasmicMembrane105
MGLC_ECOLIP23200
mglC
b2148 JW2135 integral IM protein (SEC in IM), Galactoside transport system permease MglC Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN330.106TMH05.37465-98Ni12-31o43-64i105-129o135-155i175-201o227-248i269-299o311-330iCytoplasmicMembrane10
HYFB_ECOLIP23482
hyfB

yffF

yffFb2482 JW2467 integral IM protein (SEC in IM), Hydrogenase-4 component B HyfBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECnoyesN570.166TMH07.57984-1616No6-27i39-62o82-103i110-129o135-154i166-185o205-224i245-267o273-296i303-326o332-363i383-405o425-452i473-496o532-551i652-671oCytoplasmicMembrane10
FECR_ECOLIP23485
fecR
b4292 JW4252 integral IM protein (SEC in IM), FecRIntegral Inner Membrane The protein is oriented such that residues 1 to 85 are in the cytoplasm, residues 86 to 100 form a transmembrane segment, and residues 101 to 317 are in the periplasm where the C-terminus can interact with the N-domain of the outer membrane protein FecA.PMID: 9573190 PMID: 12057967Integral Inner MembraneB-IM (predicted-detected)PeriplasmIntegral Membrane ProteinSRPSECnoyesN350.136CYT0-0.200913-00NiPeriplasmic10
ECOT_ECOLIP23827
eco

eti

etib2209 JW2197 periplasmic protein (SEC through IM), EcoPeriplasmic Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY210.889SpI2016.5012-00Yn4-15c20/21oPeriplasmic1085
PHOQ_ECOLIP23837
phoQ
b1129 JW1115 integral IM protein (SEC in IM), Sensor PhoQIntegral Inner Membrane PMID: 23359663 PMID: 15919996 Integral Inner MembraneBBinout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN230.287TMH06.14229-21Yn17-28c33/34o194-216iCytoplasmicMembrane1020
PDEA_ECOLIP23842
pdeA

yfeA

yfeAb2395 JW5391 integral IM protein (SEC in IM), Probable cyclic di-GMP phosphodiesterase PdeAIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECnoyesN450.113TMH09.46751-89No12-31i43-61o81-104i125-143o163-186i214-232o238-255i262-279o291-312iCytoplasmicMembrane10
OPPA_ECOLIP23843
oppA
b1243 JW1235 periplasmic protein (SEC through IM), oligopeptide-binding protein OppAPeriplasmicSecB substrate. Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (297 443) Disulfide bond-containing protein PMID: 25664544PMID: 16352602 PMID: 19953543 PMID: 9298646 PMID: 22030350 PMID: 25664544PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECBSECyesyesY270.825SpI2618.3169-10Yn8-18c26/27oPeriplasmic1073
DPPA_ECOLIP23847
dppA
b3544 JW3513 periplasmic protein (SEC through IM), dipeptide transport DppAPeriplasmicSubstrate of periplasmic disulfide oxidoreductases DsbA. Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (34 262;450 463) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 15911532 PMID: 19953543 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBA,SKPyesyesY290.638SpI2816.4393-00Yn13-23c28/29oPeriplasmic1085
TRKG_ECOLIP23849
trkG
b1363 JW1358 integral IM protein (SEC in IM), Trk system potassium uptake TrkG Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)yesnoN320.135TMH011.2875-1111Ni12-34o40-60i72-93o139-164i185-206o239-263i275-296o336-356i399-418o424-446i458-483oCytoplasmicMembrane10
PSPE_ECOLIP23857
pspE
b1308 JW1301 periplasmic protein (SEC through IM), Thiosulfate sulfurtransferase PspE PeriplasmicExperimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 9868784 Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 1712397 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECProphage TransposaseyesnoY200.9SpI1920.0627-00Yn5-15c19/20oUnknown2
PRC_ECOLIP23865
prc

tsp

tspb1830 JW1819 periplasmic peripheral IM protein (SEC through IM), Tail-specific protease PrcPeripheral inner membrane protein facing the periplasm Fractionation of the maxicells producing the product or prc gene suggested that the product was associated with the periplasmic side of the cytoplasmic membrane. The prc gene, which is involved in cleavage of the C-terminal peptide from the precursor form of penicillin-binding protein 3 (PBP 3) of Escherichia coli. PMID: 1856173 PMID: 1729701PMID: 1856173 PMID: 1729701Peripheral inner membrane protein facing the periplasmF2N/ACell inner membrane; Peripheral membrane protein; Periplasmic sidePeriplasmic (Experimental)SECyesyesY230.786SpI2211.2952-00Yn7-17c22/23oCytoplasmicMembrane10
FEPD_ECOLIP23876
fepD
b0590 JW0582 integral IM protein (SEC in IM), Ferric enterobactin transport system permease FepD Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.205TMH01.30983-99No6-30i64-81o93-114i121-139o151-172i193-213o239-264i284-304o310-326iCytoplasmicMembrane10
FEPG_ECOLIP23877
fepG
b0589 JW0581 integral IM protein (SEC in IM), Ferric enterobactin transport system permease FepG Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.416TMH010.9363-88Yn8-23c41/42o65-83i95-112o118-138i145-167o187-211i236-262o274-296i303-324oCytoplasmicMembrane10
CYDC_ECOLIP23886
cydC

mdrA mdrH surB

mdrA mdrH surBb0886 JW0869 integral IM protein (SEC in IM), ATP-binding/permease CydCIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN500.199TMH016.2776-66Ni16-35o41-61i133-155o161-182i243-266o278-301iCytoplasmicMembrane1010
CADC_ECOLIP23890
cadC
b4133 JW4094 nucleoid & integral IM protein (SEC in IM), Transcriptional activator CadCIntegral Inner Membrane, NucleoidMembrane-integrated pH sensor. The membrane-integrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP.PMID: 23138451 PMID: 24056175 PMID: 21890895 PMID: 18086202Integral Inner Membrane, NucleoidB, NIM (predicted)Cell membrane Membrane anchoredSRPSECnoyesN500.131CYT0-0.200913-11No159-181iCytoplasmicMembrane9
HTPX_ECOLIP23894
htpX
b1829 JW1818 integral IM protein (SEC in IM), Protease HtpXIntegral Inner Membrane PMID: 12081643 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN350.141TMH012.8648-43Yn4-18c23/24o33-53i158-178o193-217iCytoplasmicMembrane108
EMRE_ECOLIP23895
emrE

eb mvrC

eb mvrCb0543 JW0531 integral IM protein (SEC-YidC in IM), Multidrug transporter EmrE Integral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC. Dual topology membrane protein: can insert into the membrane in both directions. PMID: 15919996PMID: 22108384 PMID: 15919996 PMID: 24688744 PMID: 22178925 PMID: 16429150 PMID: 20508091 PMID: 22444835Integral Inner MembraneBBpolyin/out, out/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSEC-YidCProphage Integrase (EcoGene), pseudogene (Ochman & Davalos, 2006)nonoN620.162TMH06.32256-43No30-48i60-79o85-104iCytoplasmicMembrane107
NLPC_ECOLIP23898
nlpC
b1708 JW1698 OM lipoprotein (SEC through IM), inserted in OM by LOL), Probable endopeptidase NlpC Outer Membrane Lipoprotein Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY210.325SpII1516.0698Pos+2=S00Yn3-13c17/18oUnknown234
ARAJ_ECOLIP23910
araJ
b0396 JW0386 integral IM protein (SEC in IM), Putative transporter AraJ Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN190.21TMH04.92502-1211Yn4-15c20/21o44-63i70-96o102-120i127-150o156-178i199-219o225-251i263-284o290-310i331-350o356-375iCytoplasmicMembrane10
LNT_ECOLIP23930
lnt

cutE

cutEb0657 JW0654 integral IM protein (SEC in IM), ApoN-acyltransferase LntIntegral Inner Membrane PMID: 2032623 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSECyesyesN280.184TMH013.3696-68Ni12-28o34-50i57-79o91-112i124-142o162-188i195-214o489-507iCytoplasmicMembrane102
ENTS_ECOLIP24077
entS

ybdA

ybdAb0591 JW0583 integral IM protein (SEC in IM), Enterobactin exporter EntSIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.337TMH010.2914-1112Ni21-42o54-74i86-105o111-135i147-170o176-199i219-246o258-276i288-305o311-336i357-374o380-399iCytoplasmicMembrane10
ACRD_ECOLIP24177
acrD

yffA

yffAb2470 JW2454 integral IM protein (SEC-YidC in IM), Probable aminoglycoside efflux pump AcrDIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN350.15SpI345.86063-1111Yn10-21c26/27o340-359i366-387o393-413i438-458o470-497i538-558o873-890i897-917o923-944i972-992o1004-1026iCytoplasmicMembrane10
ACRE_ECOLIP24180
acrE

envC

envCb3265 JW3233 IM lipoprotein (SEC through IM), inserted in OM by LOL), Multidrug export AcrE Inner Membrane LipoproteinProven pignal peptidase II substrate.PMID: 8472900 PMID: 15174130Inner Membrane LipoproteinEN/ACell inner membrane Inner membrane lipoproteinSECLOLyesyesY230.241SpII2310.9032Pos+2=N00Yn7-18c23/24oCytoplasmicMembrane970
ACRF_ECOLIP24181
acrF

envD

envDb3266 JW3234 integral IM protein (SEC in IM), Multidrug export AcrF Integral Inner Membrane PMID: 11274125 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN270.286SpI2612.2125-1211Yn10-21c26/27o340-359i366-390o396-416i437-458o470-497i541-558o872-891i898-918o924-948i969-990o1002-1028iCytoplasmicMembrane10
FDNG_ECOLIP24183
fdnG
b1474 JW1470 periplasmic peripheral IM protein (TAT through IM), Formate dehydrogenase, nitrate-inducible, major subunit FdnGPeripheral inner membrane protein facing the periplasmTAT secretion system; Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site. FdnG is the catalytic subunit of formate dehydrogenase-N (FdnGHI). FdnG was found in the periplasmic fraction of a mutant lacking the membrane anchor subunit FdnI, confirming that FdnG is located at the periplasmic face of the cytoplasmic membrane. PMID: 11929547 PMID: 11884747 PMID: 12923080 PMID: 11884747 PMID: 16858726PMID: 17218314 PMID: 15342602 PMID: 11929547 PMID: 12923080 PMID: 11884747 PMID: 16858726Peripheral inner membrane protein facing the periplasmF2N/APeriplasm Membrane associated (Experimental)TATnoyesN340.342SpI336.74593-00Yn11-24c33/34oPeriplasmic10
LPXM_ECOLIP24205
lpxM

msbB

msbBb1855 JW1844 integral IM protein (SEC in IM), Lipid A biosynthesis myristoyltransferase LpxMIntegral Inner MembraneBBinout/inIM (predicted)Cell inner membrane Membrane anchoredSRPSECyesyesN450.121CYT0-0.200913-11No24-43iCytoplasmicMembrane1016
PHEP_ECOLIP24207
pheP
b0576 JW0565 integral IM protein (SEC in IM), Phenylalanine-specific permease PhePIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN530.106TMH01.83401-1212Ni30-49o55-71i107-130o136-156i163-184o204-228i249-271o291-308i340-361o367-387i408-428o434-452iCytoplasmicMembrane10
FLID_ECOLIP24216
fliD

flaV flbC

flaV flbCb1924 JW1909 Flagellar extra-cellular peripheral OM protein (Flagellum secreted), hook-associated 2 FliDPeripheral outer membrane protein facing the extra-cellular spaceFilament capping protein. PMID: 7551038 PMID: 1527488 PMID: 6305924PMID: 7551038 PMID: 1527488 PMID: 6305924Peripheral outer membrane protein facing the extra-cellular spaceF4N/ASecreted Bacterial flagellumCell surface appendage (Experimental)flagellumFlagellumFlanoyesN250.12CYT0-0.200913-00NiExtracellular1093
DACB_ECOLIP24228
dacB
b3182 JW3149 periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacBPeripheral inner membrane protein facing the periplasmExported from cytosol and signal peptide is cleaved. Anchored via C terminal the rest of the protein protudes into periplasm. Involved in peptidoglycan biosynthesis and is Penicillin-binding. PMID: 16411754 PMID: 15576782 DacB(PBP-4) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. PMID: 9371419PMID: 12444970 PMID: 15911532 PMID: 16411754 PMID: 15576782 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2N/APeriplasm Membrane anchored (Experimental)SECyesyesY210.58SpI2013.4163-00Yn6-16c20/21oPeriplasmic934
PTIBC_ECOLIP24241
ascF
b2715 JW5435 integral IM protein (SEC-YidC in IM), PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component AscFIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSEC-YidCnoyesN250.128CYT0-0.200913-810Ni104-127o147-167i179-197o217-234i254-282o288-309i330-350o362-382i389-413o433-456iCytoplasmicMembrane107
TEHA_ECOLIP25396
tehA
b1429 JW1425 integral IM protein (SEC in IM), Tellurite resistance TehA Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.119TMH012.0389-810Ni12-31o43-64i76-97o103-121i141-160o166-184i196-218o230-249i256-274o286-310iCytoplasmicMembrane10
GABP_ECOLIP25527
gabP
b2663 JW2638 integral IM protein (SEC in IM), GABA permease GabPIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.302TMH08.50646-1212Ni21-40o46-66i97-118o124-144i156-180o200-220i241-264o284-308i335-354o360-380i401-423o429-449iCytoplasmicMembrane10
ASLA_ECOLIP25549
aslA

atsA

atsAb3801 JW3773 periplasmic protein (SEC through IM), Arylsulfatase AslAPeriplasmicGN/APeriplasm PeriplasmicSECnoyesY250.743SpI2417.5099-00Yn10-20c24/25oUnknown625
YIDC_ECOLIP25714
yidC
b3705 JW3683 integral IM protein (SEC in IM), insertase YidCIntegral Inner Membrane YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase. The first periplasmic region of YidC include residues 24???346. Residues 215???265 of YidC are sufficient for SecF binding. The interaction of YidC with SecF is though not critical for cell viability as YidC, lacking residues 24-264, was fully functional to support the growth of <i>E. coli</i>. The integration of YidC into the cytoplasmic membrane requires the signal recognition particle, SecA and SecYEG.PMID: 10675323 PMID: 17073462 PMID: 11777926 PMID: 11821429 PMID: 16079137 PMID: 15919657Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRP (exp)SECyesyesN250.321SpI2412.0898-44Yn7-17c24/25o354-373i417-440o464-482i494-519oCytoplasmicMembrane1026
AMY1_ECOLIP25718
malS
b3571 JW3543 periplasmic protein (SEC through IM), alpha-amylase MalSPeriplasmic Disulfide bridge (57 75;121 537) PMID: 9268356 Disulfide bond-containing protein PMID: 25664544PMID: 1544897 PMID: 9268356 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY180.764SpI1714.5348-00Yn3-11c17/18oPeriplasmic1025
END1_ECOLIP25736
endA
b2945 JW2912 periplasmic protein (SEC through IM), Endonuclease-1 EndAPeriplasmic Identified DsbA substrate PMID: 19953543 PMID: 7867949 Disulfide bond-containing protein PMID: 25664544PMID: 4955462 PMID: 19953543 PMID: 7867949 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECDSBAnoyesY230.883SpI2222.5344-00Yn5-16c22/23oUnknown599
LYSP_ECOLIP25737
lysP

cadR

cadRb2156 JW2143 integral IM protein (SEC in IM), Lysine-specific permease LysPIntegral Inner Membrane Lysine-specific permease LysP and the membrane-integrated pH sensor CadC induce lysine-dependent adaptation of E. coli under acidic stress. In absence of lysine or high low pH LysP and CadC form stable complex. Probably CadC is inhibited by LysP via intramembrane and periplasmic contacts under non-inducing conditions. The membrane-integrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP.PMID: 15919996 PMID: 24056175 PMID: 18086202Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN470.175TMH010.7386-1212Ni21-43o49-66i103-125o131-149i161-182o202-224i245-267o287-312i348-365o371-394i415-435o447-467iCytoplasmicMembrane10
YCHE_ECOLIP25743
ychE
b1242 JW1229 integral IM protein (SEC in IM), UPF0056 YchEIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN420.169TMH010.9574-66No12-32i52-72o78-97i118-141o147-168i189-210oCytoplasmicMembrane1013
MDTG_ECOLIP25744
mdtG

yceE

yceEb1053 JW1040 integral IM protein (SEC in IM), Multidrug resistance MdtGIntegral Inner Membrane PMID: 11566977Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane Integral Membrane Protein(experimental)SRPSECnoyesN320.145TMH016.0858-1010Ni16-40o52-74i86-104o110-130i142-162o174-193i220-245o257-278i290-308o374-393iCytoplasmicMembrane10
YEIB_ECOLIP25747
yeiB
b2152 JW2139 integral IM protein (SEC in IM), YeiBIntegral Inner MembraneBBpolyin/inIM (predicted)Cell membrane Integral Membrane ProteinSRPSECyesyesN320.167TMH09.38597-1010Ni9-28o48-71i83-101o107-122i134-153o201-221i233-254o274-292i312-332o338-359iCytoplasmicMembrane10
FLIF_ECOLIP25798
fliF

fla AII.1 fla BI

fla AII.1 fla BIb1938 JW1922 Flagellar integral IM protein (SEC in IM), M-ring protein FliFIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinflagellumSRPSECnoyesN460.141SpI433.78777-22Ni23-43o451-468iCytoplasmicMembrane1054
LOIP_ECOLIP25894
loiP

yggG

yggGb2936 JW2903 OM lipoprotein (SEC through IM), inserted in OM by LOL), Metalloprotease LoiP Outer Membrane Lipoprotein Disulfide bridge (53 108) PMID: 22491786PMID: 17909889 PMID: 22491786 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane Outer membrane LipoproteinSECLOLyesyesN350.146SpII1825.8287Pos+2=Q00Yn5-16c34/35oUnknown260
YDBD_ECOLIP25907
ydbD
b1407 JW5221 periplasmic protein (SEC through IM), YdbDPeriplasmicGN/APeriplasmicSECnoyesY250.744SpI247.29978-00Yn12-20c24/25oCytoplasmicMembrane928
LEP4_ECOLIP25960
gspO

hofD hopD hopO yheC

hofD hopD hopO yheCb3335 JW3297 integral IM protein (SEC in IM), Type 4 prepilin-like proteins leader peptide-processing enzyme GspOIntegral Inner Membrane Part of gsp locus. PMID: 15919996PMID: 11118204 PMID: 15919996Integral Inner MembraneBBpolyin/outIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN240.153TMH00.982152-75Yn4-15c21/22o55-84i104-122o128-149i161-191o203-222iCytoplasmicMembrane10
BGLH_ECOLIP26218
bglH

yieC

yieCb3720 JW3698 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Cryptic porin BglHOuter Membrane b-barrel protein Reconstitution experiments with lipid bilayer membranes defined BglH as a channel-forming component, i.e. it is an outer membrane porin. Can be easily removed from the outer membrane by treatment with detergent. PMID: 10027967 PMID: 10027967PMID: 10027967Outer Membrane b-barrel proteinHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECBAMnoyesY260.64SpI2513.3498-00Yn6-17c25/26oOuterMembrane108
FEPE_ECOLIP26266
fepE
b0587 JW0579 integral IM protein (SEC in IM), Ferric enterobactin transport FepE Integral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN460.11CYT0-0.200913-22Ni42-61o339-362iCytoplasmicMembrane1033
AMIB_ECOLIP26365
amiB

yjeD

yjeDb4169 JW4127 periplasmic protein (SEC through IM), N-acetylmuramoyl-L-alanine amidase AmiB PeriplasmicPMID: 12787347PeriplasmicGN/APeriplasm Periplasmic (Experimental)SECyesyesY230.861SpI2213.4392-00Yn9-17c22/23oUnknown230
APPB_ECOLIP26458
appB

cbdB cyxB

cbdB cyxBb0979 JW0961 integral IM protein (SEC in IM), Cytochrome bd-II ubiquinol oxidase subunit 2 AppBIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECyesyesN410.107TMH010.1949-89No12-40i61-79o85-103i123-148o168-188i209-233o263-283i290-314o334-356iCytoplasmicMembrane10
APPC_ECOLIP26459
appC

cbdA cyxA

cbdA cyxAb0978 JW0960 integral IM protein (SEC-YidC in IM), Cytochrome bd-II ubiquinol oxidase subunit 1 AppCIntegral Inner Membrane Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner MembraneBBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN370.11TMH011.9815-99No20-42i54-71o91-116i128-145o184-208i215-235o386-409i421-442o471-492iCytoplasmicMembrane10
PLSC_ECOLIP26647
plsC

parF

parFb3018 JW2986 integral IM protein (SEC in IM), 1-acyl-sn-glycerol-3-phosphate acyltransferase PlsCIntegral Inner Membrane PMID: 1557036Integral Inner MembraneBBoutin/outIM (predicted)Cell inner membrane Membrane anchored (Experimental)SECyesyesN360.111TMH01.55803-11No6-27iCytoplasmicMembrane1028
FTSP_ECOLIP26648
ftsP

sufI

sufIb3017 JW2985 periplasmic protein (TAT-SEC through IM), Cell division FtsPPeriplasmicTAT secretion system. PMID: 9298646PMID: 17218314 PMID: 10593889 PMID: 19135451 PMID: 9298646 PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECnoyesY280.494SpI2710.6124-00Yn8-19c27/28oPeriplasmic108
RHAT_ECOLIP27125
rhaT
b3907 JW3878 integral IM protein (SEC in IM), L-rhamnose-proton symporter RhaTIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyout/outIM (predicted) , Soluble IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN200.227TMH012.7916-1010No6-26i38-58o64-85i97-121o133-154i175-194o214-234i255-277o289-310i322-343oCytoplasmicMembrane10
RFAL_ECOLIP27243
rfaL

waaL

waaLb3622 JW3597 integral IM protein (SEC in IM), O-antigen ligase RfaLIntegral Inner Membrane PMID: 15919996Integral Inner MembraneBBpolyin/inIM (predicted)Cell inner membrane; Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN370.114TMH00.404949-1212Ni20-38o44-60i72-91o103-122i134-152o172-189i196-212o218-233i240-258o349-368i375-393o399-415iCytoplasmicMembrane10
LPXK_ECOLIP27300
lpxK

ycaH

ycaHb0915 JW0898 integral IM protein (SEC in IM), Tetraacyldisaccharide 4'-kinase LpxKIntegral Inner MembraneBBinout/inIM (predicted)Integral Membrane ProteinSRPSECnoyesN440.107TMH01.00613-11No12-34iUnknown230
EMRA_ECOLIP27303
emrA
b2685 JW2660 integral IM protein (SEC in IM), Multidrug export EmrA Integral Inner Membrane PMID: 1409590 PMID: 12482849Integral Inner MembraneBBoutin/outSoluble IM FractionCell inner membrane Membrane anchoredSRPSECyesyesN380.157TMH06.79297-11Ni23-44oCytoplasmicMembrane1025
RODZ_ECOLIP27434
rodZ

yfgA

yfgAb2516 JW2500 nucleoid & integral IM protein (SEC in IM), Cytoskeleton RodZIntegral Inner Membrane, NucleoidSignal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. Quantitative immunoblotting agaist RodZ purified protein indicate that RodZ is present at ~650 copies per average exponentially growing cell in LB medium and M9. The hth domain of RodZ is not required for transcription but for interaction with MreB. The protein, RodZ, is required for assembly of the actin cytoskeleton MreB that controls cell wall synthesis and cell shape. PMID: 19194484PMID: 23138451 PMID: 9008159 PMID: 19078962 PMID: 21890895 PMID: 19008860 PMID: 15919657 PMCID: PMC2637328 PMID: 19194484Integral Inner Membrane, NucleoidB, NIM (predicted)Cell inner membrane Membrane anchoredSECyesyesN350.243CYT0-0.200913-11Ni112-133oUnknown2