entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
YKFM_ECOLIA5A605
ykfM
b4586 L-fucose-proton symporter (6-deoxy-L-galactose permease) (L-fucose permease)Integral Inner Membrane BBpolyin/outIM (predicted)Membrane | Multi-pass membrane protein-SRPSECnoyesN270.205TMH013.6098-330i12-32o52-75i96-119oCytoplasmicMembrane10
YMGJ_ECOLIA5A612
ymgJ
b4594 Uncharacterized membrane protein ypdKWeak signal peptide prediction. Function Unkown.PeriplasmicGIM (predicted)-SECnoyesN240.144CYT0-0.200913-00Yn6-17c22/23oUnknown2
YNCL_ECOLIA5A615
yncL
b4598 JW1439.1 Putative O-antigen transporterInner membrane.Small membrane protein detected experimentally. Found to co-fractionate with the inner membrane.PMID: 19121005 PMID: 21778229Integral Inner Membrane BBinoutin/outIM (predicted)Membrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN270.099CYT0-0.200913-110i7-29oCytoplasmicMembrane9.82
YNEM_ECOLIA5A616
yneM
b4599 JW1527.1 Uncharacterized protein yneMInner membrane (experimental). Inner membrane (experimental).Found to co-fractionate with the inner membrane. Experimentally verified orientation: N(out)-C(in).PMID: 19121005 PMID: 21778229Integral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN240.123CYT0-0.200913-110i7-25oCytoplasmicMembrane9.82
YDGU_ECOLIA5A617
ydgU
b4601 JW1589 Uncharacterized protein yfbLSmall membrane protein detected experimentally.PMID: 19121005Integral Inner Membrane BBinoutin/outIM (predicted)-SRPSECnoyesN270.097CYT0-0.200913-110i7-26oUnknown2
YNHF_ECOLIA5A618
ynhF
b4602 JW1649.1 O-antigen polymeraseInner membrane (experimental). Inner membrane (experimental).Found to co-fractionate with the inner membrane. Can be inserted in both orientations (Cout-Nin and Cin-Nout).PMID: 19121005 PMID: 21778229 PMID: 21778229 PMID: 21778229Integral Inner Membrane BBinout, Bmultiin/out, out/inIM (predicted)Membrane | Single-pass membrane protein-SRPSECnoyesN270.105CYT0-0.200913-110i7-28oCytoplasmicMembrane9.82
DINQ_ECOLIA5A624
dinQ
b4613 Uncharacterized protein DinQIntegral Inner Membrane BBinoutin/outIM (predicted)Membrane | Single-pass membrane protein-SRPSECnoyesN310.097CYT0-0.200913-110i20-38oUnknown2
TISB_ECOLIA5A627
tisB

ysdB

ysdBb4618 JW3649 Small toxic protein tisB (LexA-regulated protein tisB)Cell inner membranePotentially toxic protein.PMID: 19121005 PMID: 18761622Integral Inner Membrane BBinoutout/inIM (predicted)Cell inner membrane | Single-pass membrane protein-SRPSECpseudogene (Uniprot), pseudogene (Ecogene)nonoN230.099CYT0-0.200913-110o6-25iUnknown2
YJBT_ECOLIA5A628
yjbT
b4620 Uncharacterized protein yjbTBelongs to the yjbT family.PeriplasmicGN/A-SECnoyesY300.744SpI2915.6556-10Yn15-25c29/30oPeriplasmic9.84
YTCA_ECOLIA5A630
ytcA
b4622 Uncharacterized protein ytcAIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Lipid-anchorMembrane | Multi-pass membrane proteinIntegral Membrane ProteinSECnoyesN330.139TMH010.1309-22Yn16-27c32/33o42-61i68-90oCytoplasmicMembrane10
YTHA_ECOLIA8DYQ1
ythA
b4655 JW5769.1 Uncharacterized protein ythASmall membrane protein detected experimentallyPMID: 19121005Integral Inner Membrane BBinin/outN/ACell membrane | Single-pass membrane protein-SECnoyesN410.12CYT0-0.200913-110i21-40oCytoplasmicMembrane9.82
YKGR_ECOLIC1P5Z8
ykgR
b4671 JW5035.1 Uncharacterized membrane protein ykgRIntegral inner membrane (experimental).Found to co-fractionate with the inner membrane. Experimentally verified orientation: N(in)-C(out).PMID: 19121005 PMID: 21778229Integral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane protein-SRPSECnoyesN210.096CYT0-0.200913-110i12-30oUnknown2
YMIB_ECOLIC1P5Z9
ymiB
b4672 JW1278.1 Putative protein ymiBIntegral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane protein-SRPSECnoyesN280.1CYT0-0.200913-110i12-29oUnknown2
RZOQ_ECOLIC1P601
rzoQ
b4689 JW1545.1 Uncharacterized J domain-containing protein djlCInner Membrane LipoproteinEN/ACell membrane | Lipid-anchor-SECLOLnoyesN290.129SpII2211.4311Pos+2=V00Yn9-20c25/26oUnknown2
YOAK_ECOLIC1P602
yoaK
b4676 JW5292.1 Inner membrane protein ybhIInner membrane (experimental). Inner membrane (experimental).Found to co-fractionate with the inner membrane. Can be inserted in both orientations (Cout-Nin and Cin-Nout). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229 PMID: 21778229 PMID: 21778229Integral Inner Membrane BBinout, Bmultiin/out, out/inN/AMembrane | Single-pass membrane protein-SRPYidCnoyesN290.152TMH03.55641-110o6-29iCytoplasmicMembrane9.82
YOAJ_ECOLIC1P603
yoaJ
b4675 JW5292.2 Uncharacterized protein YoaJInner Membrane.Found to co-fractionate with the inner membrane. Experimentally verified orientation: N(in)-C(out). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229 PMID: 21778229Integral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane protein-SRPYidCnoyesN190.098CYT0-0.200913-110o6-23iCytoplasmicMembrane9.82
YOHP_ECOLIC1P609
yohP
b4679 JW5358.1 Inner membrane transport protein ydiMIntegral inner membrane (experimental). Integral inner membrane (experimental).Found to co-fractionate with the inner membrane. Can be inserted in both orientations (Cout-Nin and Cin-Nout).PMID: 19121005 PMID: 21778229Integral Inner Membrane BBinout, Bmultiin/out, out/inN/AMembrane | Single-pass membrane protein-SRPSECnoyesN250.1TMH00.568556-110o6-26iCytoplasmicMembrane9.82
YPDK_ECOLIC1P610
ypdK
b4680 JW2375.1 Uncharacterized transporter YeaVOuter membrane Inner membrane.Found to co-fractionate with the inner membrane.PMID: 19121005 PMID: 21778229Integral Inner Membrane BBinoutout/inN/AMembrane | Single-pass membrane protein-SRPSECnoyesN170.106CYT0-0.200913-110o6-22iCytoplasmicMembrane9.82
SHOB_ECOLIC1P611
shoB

ryfB yphI

ryfB yphIb4687 JW2546.1 IlvBN operon leader peptide (IlvBN operon attenuator peptide)Inner membranePMID: 19121005 PMID: 18710431Integral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane protein-SRPSECnoyesN130.098CYT0-0.200913-110i7-25oCytoplasmicMembrane9.82
YQFG_ECOLIC1P614
yqfG
b4684 JW5470.1 Uncharacterized protein yqfGSmall protein detected experimentally.PMID: 19121005Integral Inner Membrane B-N/A-SRPSECnoyesN240.133TMH02.01664-10Yn7-17c29/30oCytoplasmicMembrane9.82
IBSD_ECOLIC1P616
ibsD
b4664 JW3023.1 Autoinducer 2 import system permease protein lsrD (AI-2 import system permease protein lsrD)Belongs to the toxic protein ibs family. Potentially toxic protein.PMID: 19121005Integral Inner Membrane B-N/AMembrane | Single-pass membrane protein-SECnoyesN180.098CYT0-0.200913-10Yn4-9c13/14oCytoplasmicMembrane9.82
PMRR_ECOLIG3MTW7
pmrR
b4703 JW4072.1Putative membrane protein PmrR Integral Inner Membrane BBoutin/outN/ASECno1Unknown2
YPDI_ECOLIO32528
ypdI
b2376 JW2373 Uncharacterized lipoprotein ypdIOuter Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane LipoproteinSECLOLnoyesY240.415SpII1812.5879Pos+2=N10Yn5-16c23/24oUnknown222.88
YFJS_ECOLIO52982
yfjS
b2636 JW5921 Uncharacterized lipoprotein yfjSInner Membrane LipoproteinEN/ACell membrane | Lipid-anchorInner membrane lipoproteinSECLOLProphage Integrase (EcoGene)nonoY210.479SpII2018.7233Pos+2=D00Yn6-17c21/22oUnknown2.5
GSPB_ECOLIP03825
gspB

pinO pioO pno

pinO pioO pnob3322 JW3284 Putative general secretion pathway protein BIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECnoyesN430.123TMH03.27755-110o28-51iUnknown2
BTUE_ECOLIP06610
btuE
b1710 JW1700 Vitamin B12 transport periplasmic protein BtuE (EC 1.-.-.-) Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmCytoplasmicSEC, non classicalyesyesN370.111CYT0-0.200913-000iPeriplasmic1099.42
SODM_ECOLIP00448
sodA
b3908 JW3879 Superoxide dismutase [Mn] (EC 1.15.1.1) (MnSOD)PeriplasmicThe superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are E.coli proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm.PMID: 21854464PeriplasmicGN/ACytoplasmicSEC, non classicalyesyesN510.145CYT0-0.200913-000iUnknown5.41
PHSM_ECOLIP00490
malP
b3417 JW5689 Maltodextrin phosphorylase (EC 2.4.1.1)Peripheral inner membrane protein facing the cytoplasm (potential).PMID: 15919657Integral Inner Membrane B-Soluble IM FractionCytoplasmicSECyesyesN540.131CYT0-0.200913-000iCytoplasmic9.97
PTM3C_ECOLIP00550
mtlA
b3599 JW3573 PTS system mannitol-specific EIICBA component (EIICBA-Mtl) (EII-Mtl) [Includes: Mannitol permease IIC component (PTS system mannitol-specific EIIC component); Mannitol-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (PTS system mannitol-speInner membrane (experimental). Integral inner membrane (experimental). Inner membrane (experimental). Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. The signal anchor sequence of MtlA confers novel properties onto nascent chains of OmpA of being co-translationally recognized and targeted to SecY by SRP. PMCID: PMC305875 The signal anchor sequence of MtlA confers novel properties onto nascent chains of OmpA of being co-translationally recognized and targeted to SecY by SRP. Once targeted to SecY, ribosome-associated nascent chains of the hybrid protein, however, remain untranslocated unless SecA is present. These results indicate that SRP and SecA cooperate in a sequential, non-overlapping manner. PMCID: PMC305875PMID: 9008159 PMID: 15919657 PMID: 15919996 PMID: 9260961 PMCID: PMC305875Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN230.115TMH011.9934-880i20-42o48-66i78-98o133-154i161-181o213-234i269-292o312-334iCytoplasmicMembrane1026.94
YGDB_ECOLIP08370
ygdB
b2824 JW5450 Uncharacterized protein ygdBPeriplasmicGN/APeriplasmicSECyesyesN270.147SpI267.55205-10Yn10-23c35/36oUnknown2.57
PPDB_ECOLIP08371
ppdB

ygdC

ygdCb2825 JW5451 Prepilin peptidase-dependent protein BIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECyesyesN360.136CYT0-0.200913-110o13-34iUnknown223.18
PPDC_ECOLIP08372
ppdC

ygdA

ygdAb2823 JW2791 Prepilin peptidase-dependent protein CIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECyesyesN300.122TMH06.3414-110o16-33iUnknown224.18
PPB_ECOLIP00634
phoA
b0383 JW0374 Alkaline phosphatase (APase) (EC 3.1.3.1)Periplasm Periplasmic Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 3533724 PMID: 19953543 PMID: 7022451 PMID: 3009404 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAnoyesY220.408SpI213.05238-00Yn5-16c21/22oPeriplasmic1078.02
LEP_ECOLIP00803
lepB
b2568 JW2552 Signal peptidase I (SPase I) (EC 3.4.21.89) (Leader peptidase I)Integral inner membrane (experimental). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased. Lep was shown to be targeted to the membrane by the SRP pathway. PMID: 8985168 Disulfide bridge (171 177) PMID: 2551889 PMID: 21778229 PMID: 15919996 PMID: 8985168 Integral Inner Membrane Bout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinYidCyesyesN220.131SpII203.04951Pos+2=V20Yn5-16c21/22oCytoplasmicMembrane1027.67
LSPA_ECOLIP00804
lspA
b0027 JW0025 Lipoprotein signal peptidase (EC 3.4.23.36) (Prolipoprotein signal peptidase) (Signal peptidase II) (SPase II)Cell inner membrane Integral inner membrane (experimental).Catalyzes the removal of signal peptides from prolipoproteins.PMID: 6378662 PMID: 6368552 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN360.135TMH07.83163-440i12-31o70-88i100-117o137-157iCytoplasmicMembrane105.54
ASPG2_ECOLIP00805
ansB
b2957 JW2924 L-asparaginase 2 (EC 3.5.1.1) (L-asparaginase II) (L-ASNase II) (L-asparagine amidohydrolase II) (Colaspase)Periplasmic Periplasmic Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (99 127) PMID: 8434007 Disulfide bond-containing protein PMID: 25664544PMID: 387570 PMID: 2407723 PMID: 25403562 PMID: 8434007 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.82SpI2219.7315-10Yn7-17c22/23oPeriplasmic1049.18
CVPA_ECOLIP08550
cvpA

dedE

dedEb2313 JW2310 Colicin V production protein (Protein dedE) (Pur regulon 18 kDa protein)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN310.117TMH06.39839-440o6-25i32-52o64-87i99-120oCytoplasmicMembrane10
FTSB_ECOLIP0A6S5
ftsB

ygbQ

ygbQb2748 JW2718 Cell division protein ftsBCell inner membrane Cell inner membranePMID: 11972052 PMID: 15165235Integral Inner Membrane BBoutout/inIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchoredSRPSECyesyesN230.157CYT0-0.200913-10Yn4-14c18/19oCytoplasmicMembrane9.8225.62
ISPZ_ECOLIP0A710
yciB

ispZ

ispZb1254 JW1246 Probable intracellular septation proteinIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN250.173TMH07.58982-550o20-39i51-68o74-96i117-139o151-170iCytoplasmicMembrane10
AMPC_ECOLIP00811
ampC

ampA

ampAb4150 JW4111 Beta-lactamase (EC 3.5.2.6) (Cephalosporinase)PeriplasmicPMID: 6795623PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.854SpI1917.1519-00Yn4-15c19/20oPeriplasmic1026.22
MALF_ECOLIP02916
malF
b4033 JW3993 Maltose transport system permease protein malFCell inner membrane, Periplasmic side Integral inner membrane (experimental). Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. Crystal structure of a catalytic intermediate of the maltose transporter. PMID: 18033289 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 9008159 PMID: 18456666 PMID: 15919996 PMID: 18033289 PMID: 25403562 PMID: 25403562Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane protein | Periplasmic sideIntegral Membrane ProteinSRP (exp)SECYfgMnoyesN650.175TMH014.2023-880i14-34o40-58i67-91o277-303i315-340o371-392i426-447o484-506iCytoplasmicMembrane10
PBPA_ECOLIP02918
mrcA

ponA

ponAb3396 JW3359 Penicillin-binding protein 1A (PBP-1a) (PBP1a) [Includes: Penicillin-insensitive transglycosylase (EC 2.4.2.-) (Peptidoglycan TGase); Penicillin-sensitive transpeptidase (EC 3.4.-.-) (DD-transpeptidase)]Inner membrane (experimental). Membrane anchoredThe formation of a complex between a murein polymerase (PBP1B) and a murein hydrolase (MltA) in the presence of MipA represents a first step in a reconstitution of the hypothetical murein-synthesizing holoenzyme, postulated to be responsible for controlled growth of the stress-bearing sacculus of E. coli. The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Uniprot annotation is Cell inner membrane; Single-pass type II membrane protein, Gene Ontology and Echolocation annotation is Membrane anchored. PMID: 3510188PMID: 10037771 PMID: 21183073 PMID: 15919657 PMID: 3510188Integral Inner Membrane BBoutin/outSoluble IM FractionCell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SRPSECyesyesN250.144TMH02.26474-10Yn7-17c24/25oCytoplasmicMembrane104.59
RNFA_ECOLIP0A766
rnfA

ydgL

ydgLb1627 JW1619 Electron transport complex protein rnfAIntegral inner membrane (experimental).PMID: 15919996 PMID: 24688744Integral Inner Membrane BBpoly, Bmultiout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein(experimental)SRPSECyesyesN210.118TMH07.31415-660o6-26i38-59o65-87i99-122o134-155i167-191oCytoplasmicMembrane10
PSIE_ECOLIP0A7C8
psiE

yjbA

yjbAb4030 JW3990 Protein psiEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN430.143TMH04.00332-440i12-35o55-74i86-105o111-128iCytoplasmicMembrane10
TATE_ECOLIP0A843
tatE

ybeC

ybeCb0627 JW0622 Sec-independent protein translocase protein tatETatA paralog TatE can substitute for TatA and support translocation of Tat substrates including AmiA, AmiC, and TorA.PMID: 22190680Integral Inner Membrane BBoutout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN430.118CYT0-0.200913-10Yn9-20c29/30oCytoplasmicMembrane9.82127.03
PBPB_ECOLIP02919
mrcB

pbpF ponB

pbpF ponBb0149 JW0145 Penicillin-binding protein 1B (PBP-1b) (PBP1b) (Murein polymerase) [Includes: Penicillin-insensitive transglycosylase (EC 2.4.1.129) (Peptidoglycan TGase) (Peptidoglycan glycosyltransferase); Penicillin-sensitive transpeptidase (EC 3.4.-.-) (DD-transpeptiInner membrane (experimental).The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Cell inner membrane; Single-pass type II membrane protein. Monoisotopic peripheral; Contains amphipathic a-helix.PMID: 19458048 PMID: 21183073 PMID: 15919657Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionCell inner membrane | Single-pass type II membrane proteinMembrane anchoredSRPSECyesyesN30.105CYT0-0.200913-110i65-86oCytoplasmicMembrane10
LACY_ECOLIP02920
lacY
b0343 JW0334 Lactose permease (Lactose-proton symport) Integral inner membrane (experimental). Integral inner membrane (experimental).Crystal structure of lactose permease indicated that the molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease PMID: 12893935.PMID: 11266632 PMID: 12893935 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.125TMH018.848-11120i12-34o46-66i75-95o101-122i143-162o168-187i222-239o259-279i291-310o316-337i349-369o381-401iCytoplasmicMembrane10
MELB_ECOLIP02921
melB

mel-4

mel-4b4120 JW4081 Melibiose carrier protein (Melibiose permease) (Melibiose transporter) (Na+ (Li+)/melibiose symporter) (Thiomethylgalactoside permease II)Cell inner membrane Integral inner membrane (experimental).PMID: 7703254 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN180.118TMH08.17946-12120i12-31o37-57i78-100o106-130i150-171o177-198i236-258o270-290i297-318o324-353i374-397o409-432iCytoplasmicMembrane10
YFBV_ECOLIP0A8D9
yfbV
b2295 JW2292 UPF0208 membrane protein yfbVIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.122CYT0-0.200913-220i46-64o70-90iCytoplasmicMembrane1038.96
USPB_ECOLIP0A8S5
uspB

yhiO

yhiOb3494 JW3461 Universal stress protein BIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.13TMH00.843073-220o6-24i90-110oCytoplasmicMembrane1057.89
FLIE_ECOLIP0A8T5
fliE

fla AI flaN

fla AI flaNb1937 JW1921 Flagellar hook-basal body complex protein fliEInner membrane.MS-ring rod junction proteinPMID: 10809679Peripheral inner membrane protein facing the periplasmF2N/ABacterial flagellum basal bodyCytoplasmicflagellumFlagellum noyesN410.269CYT0-0.200913-000iPeriplasmic1062.41
ARAF_ECOLIP02924
araF
b1901 JW1889 L-arabinose-binding periplasmic protein (ABP)PeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY240.816SpI2320.0244-00Yn7-18c23/24oPeriplasmic1086.5
RBSB_ECOLIP02925
rbsB

prlB rbsP

prlB rbsPb3751 JW3730 D-ribose-binding periplasmic proteinPeriplasmic Periplasmic Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 15911532 PMID: 19953543 PMID: 9298646PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECSKPnoyesY260.839SpI2522.7093-00Yn6-17c25/26oPeriplasmic1093.52
TONB_ECOLIP02929
tonB

exbA

exbAb1252 JW5195 Protein tonBIntegral inner membrane protein.PMID: 11967085Integral Inner Membrane BBoutin/outN/ACell inner membrane | Single-pass membrane protein | Periplasmic sideMembrane anchoredSECyesyesN430.176TMH03.06055-010i12-32oUnknown2
TOLC_ECOLIP02930
tolC

mtcB mukA refI

mtcB mukA refIb3035 JW5503 Outer membrane protein tolCOuter membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein. Outer membrane protein (experimental). Outer Membrane Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinPart of MdtABC-TolC multidrug efflux transport system = [(TolC)3][MdtC][MdtB][MdtA]. Also part of MacAB-TolC macrolide efflux transport system = [(TolC)3][MacB]2[MacA]. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000).The crystal structure of MacA has been determined at 3.0A resolution. The inner membrane transporter MacB requires the outer membrane factor TolC and the periplasmic adaptor protein MacA to form a functional tripartite complex. Electron microscopic indicates that the MacA hexamer interacts with TolCIn a tip-to-tip manner. Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 12107133 PMID: 19254725 PMID: 21325274 PMID: 16372265 PMID: 16352602 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 10879525 PMID: 15228545 PMID: 16079137 PMID: 21778229 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECBSECSKPBAMyesyesY230.712SpI2210.2716-00Yn4-14c22/23oOuterMembrane1015.92
YCCT_ECOLIP0A8X4
yccT
b0964 JW0947 UPF0319 protein yccTPeriplasmicGN/APeriplasmicSECyesyesY210.531SpI206.4525-00Yn3-14c20/21oUnknown2.560.35
ESSD_ECOLIP0A9R2
essD

ybcR

ybcRb0554 JW0543 Lysis protein S homolog from lambdoid prophage DLP12Integral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)yesnoN380.193CYT0-0.200913-220i7-25o37-58iUnknown214.71
YOBA_ECOLIP0AA57
yobA
b1841 JW1830 Protein yobAPeriplasmicGN/APeriplasmPeriplasmicSECyesyesY270.511SpI2613.4937-00Yn11-21c26/27oPeriplasmic9.76
OMPF_ECOLIP02931
ompF

cmlB coa cry tolF

cmlB coa cry tolFb0929 JW0912 Outer membrane protein F (Outer membrane protein 1A) (Outer membrane protein B) (Outer membrane protein IA) (Porin OmpF)Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane (experimental) Outer Membrane Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinSubstrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 9298646PMID: 8626309 PMID: 17908933 PMID: 19343722 PMID: 22658749 PMID: 19737520 PMID: 12488465 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 1380671 PMID: 11266632 PMID: 16079137 PMID: 20932056 PMID: 10806384 PMID: 21541338 PMID: 19953543 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)TFSECSKP,SURABAMyesyesY230.883SpI2219.9098-00Yn6-17c22/23oOuterMembrane1018.18
PHOE_ECOLIP02932
phoE

ompE

ompEb0241 JW0231 Outer membrane pore protein EOuter membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinPMID: 1380671 PMID: 11266632 PMID: 20932056 PMID: 216015Outer Membrane b-barrel proteinHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECBAMyesyesY220.841SpI2121.3546-00Yn5-16c21/22oOuterMembrane102.7
MCP1_ECOLIP02942
tsr

cheD

cheDb4355 JW4318 Methyl-accepting chemotaxis protein I (MCP-I) (Serine chemoreceptor protein)Cell inner membrane Integral inner membrane (experimental).PMID: 22380631 PMID: 15919996Integral Inner Membrane BBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.141SpI255.90497-21Yn10-21c26/27o192-214iCytoplasmicMembrane1044.19
YACC_ECOLIP0AA95
yacC
b0122 JW0118 Uncharacterized protein yacCPeriplasmicGN/APeriplasmicSECyesyesY230.239SpI2410.6167-00Yn7-17c22/23oUnknown2.521.8
MATB_ECOLIP0AAA3
matB

yagZ

yagZb0293 JW0287 Fimbrillin matB homologPeriplasmicPutative chaperone–usher fimbrial operon yag (class α); Fimbrial Subunit (yagZ). PMID: 16522795PMID: 20345943 PMID: 16522795PeriplasmicGN/APeriplasmic (Experimental)fimbriaeSECnoyesY230.863SpI2225.6343-00Yn5-15c22/23oUnknown2.534.62
YMCE_ECOLIP0AAA5
sfa

sfa

sfab0991 JW0975 Protein sfaIntegral Inner Membrane BBoutin/outN/ACell membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN460.141TMH04.44945-10Yn15-25c29/30oUnknown2100.09
ZRAP_ECOLIP0AAA9
zraP

yjaI zra

yjaI zrab4002 JW5546 Zinc resistance-associated proteinPeriplasmic Heavy-metal resistance protein (IPR025961) PMID: 9694902 PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.74SpI2628.6968-10Yn7-18c26/27oPeriplasmic10
LAMB_ECOLIP02943
lamB

malB

malBb4036 JW3996 Maltoporin (Lambda receptor protein) (Maltose-inducible porin)Outer membrane protein. Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel protein Potential pubstrate of periplasmic chaperones Skp. Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000). Experimentally verified trimeric beta-barrel. N-terminal region is in periplasm. Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 346375 Disulfide bridges between 47 and 63 PMID: 1988451 Disulfide bond-containing protein PMID: 25664544PMID: 16372265 PMID: 8626309 PMCID: PMC1993877 PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 7824948 PMID: 11266632 PMID: 16079137 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 19953543 PMID: 346375 PMID: 1988451 PMID: 25664544Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSKP,SURA,DEGPBAMyesyesY260.795SpI2520.7922-00Yn8-18c25/26oOuterMembrane1054.55
KEFC_ECOLIP03819
kefC

trkC

trkCb0047 JW0046 Glutathione-regulated potassium-efflux system protein kefC (K(+)/H(+) antiporter)Cell inner membrane Integral inner membrane (experimental).PMID: 2046548 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN340.112TMH07.67646-13130o6-24i31-49o55-72i84-111o117-135i147-169o181-202i214-233o239-258i270-290o296-315i327-348o360-378iCytoplasmicMembrane10
MALM_ECOLIP03841
malM

molA

molAb4037 JW3997 Maltose operon periplasmic proteinPeriplasmicPMID: 2434655PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.606SpI2624.5925-00Yn7-18c26/27oPeriplasmic10
ATKA_ECOLIP03959
kdpA
b0698 JW0686 Potassium-transporting ATPase A chain (EC 3.6.3.12) (ATP phosphohydrolase [potassium-transporting] A chain) (Potassium-binding and translocating subunit A) (Potassium-translocating ATPase A chain)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN210.183TMH00.239367-1212Yn5-16c20/21o59-82i94-115o135-155i167-190o253-271i283-301o327-349i356-374o380-398i418-437o484-505i526-548oCytoplasmicMembrane1010.15
YKGJ_ECOLIP0AAL9
ykgJ
b0288 JW0282 Uncharacterized protein ykgJ PeriplasmicGN/APeriplasmicSECnoyesN270.286SpI244.7292-000iUnknown233.44
YAIY_ECOLIP0AAP7
yaiY
b0379 JW0370 Inner membrane protein yaiYIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.177TMH0-0.122448-220i25-48o68-95iCytoplasmicMembrane1039.97
YAIZ_ECOLIP0AAQ0
yaiZ
b0380 JW5053 Uncharacterized protein yaiZIntegral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN180.115TMH06.70305-220i12-32o38-59iCytoplasmicMembrane9.8272.46
ATKB_ECOLIP03960
kdpB
b0697 JW0685 Potassium-transporting ATPase B chain (EC 3.6.3.12) (ATP phosphohydrolase [potassium-transporting] B chain) (Potassium-binding and translocating subunit B) (Potassium-translocating ATPase B chain)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN550.195CYT0-0.200913-760i35-56o62-82i219-240o252-274i616-636o656-681iCytoplasmicMembrane108.13
ATKC_ECOLIP03961
kdpC
b0696 JW0684 Potassium-transporting ATPase C chain (EC 3.6.3.12) (ATP phosphohydrolase [potassium-transporting] C chain) (Potassium-binding and translocating subunit C) (Potassium-translocating ATPase C chain)Integral Inner Membrane BBoutin/outN/ACell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN390.2TMH011.3621-10Yn7-18c30/31oCytoplasmicMembrane1018.2
FIMA1_ECOLIP04128
fimA

pilA

pilAb4314 JW4277 Type-1 fimbrial protein, A chain (Type-1A pilin)Cell surface appendage Fim operon (class γ1); Fimbrial Subunit. Experimentally verified signal peptide PMID: 2858471 Disulfide bridge (44 84) Disulfide bond-containing protein PMID: 25664544PMID: 20345943 PMID: 6147250 PMID: 2858471 PMID: 25664544Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUyesyesY240.78SpI2325.7568-10Yn5-16c23/24oExtracellular10
LIVK_ECOLIP04816
livK
b3458 JW3423 Leucine-specific-binding protein (L-BP) (LS-BP) Periplasmic Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 9298646 PMID: 22030350 PMID: 8061609 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.817SpI2317.7516-00Yn7-18c23/24oPeriplasmic1078.71
YBAN_ECOLIP0AAR5
ybaN
b0468 JW0457 Inner membrane protein ybaNIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN490.172TMH011.6864-440i5-25o31-52i73-91o97-115iCytoplasmicMembrane1015.92
YBAV_ECOLIP0AAR8
ybaV
b0442 JW0432 Uncharacterized protein ybaVPeriplasmicGN/APeriplasmicSECyesyesY260.681SpI2523.157-00Yn7-17c25/26oCytoplasmicMembrane9.97
YLAC_ECOLIP0AAS0
ylaC
b0458 JW5063 Inner membrane protein ylaCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.106CYT0-0.200913-220i36-55o61-79iCytoplasmicMembrane1060.78
YBFA_ECOLIP0AAU2
ybfA
b0699 JW0688 Uncharacterized protein ybfAIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECnoyesN220.331TMH02.48449-110o12-33iCytoplasmicMembrane9.8212.53
NLPA_ECOLIP04846
nlpA
b3661 JW3635 Lipoprotein 28Lipoprotein (experimental). Proven signal peptidase II substrate.PMID: 15174130Inner Membrane LipoproteinESoluble IM FractionCell inner membrane | Lipid-anchorInner membrane lipoproteinSECLOLyesyesY230.355SpII2321.7987Pos+2=D00Yn10-21c26/27oCytoplasmicMembrane1077.23
FLIC_ECOLIP04949
fliC

flaF hag

flaF hagb1923 JW1908 FlagellinOuter membrane vesicles (OMVs) Cell surface appendageFlameint protein, flagellin. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type fliC, an E. coli strain with a fliC-null mutation produced reduced amounts of OMVs based on both protein and phosphate levels. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 12730325PMID: 24134841 PMID: 21541338 PMID: 12730325Peripheral outer membrane protein facing the extra-cellular spaceF4Soluble IM FractionSecretedBacterial flagellumCell surface appendage (Experimental)flagellumFlagellumFlanoyesN520.13CYT0-0.200913-000iExtracellular1080.39
PTRA_ECOLIP05458
ptrA

ptr

ptrb2821 JW2789 Protease 3 (EC 3.4.24.55) (Pitrilysin) (Protease III) (Protease pi)PeriplasmicPotential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 15502359PMID: 18498364 PMID: 15502359PeriplasmicGN/APeriplasmPeriplasmic(experimental)SECSURAyesyesY240.815SpI2313.0719-00Yn9-18c23/24oPeriplasmic1066.05
MCP3_ECOLIP05704
trg
b1421 JW1417 Methyl-accepting chemotaxis protein III (MCP-III) (Ribose and galactose chemoreceptor protein)Cell inner membrane Integral inner membrane (experimental).PMID: 22380631 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN400.259TMH07.8524-220i20-44o199-222iCytoplasmicMembrane1034.19
YBFB_ECOLIP0AAU5
ybfB
b0702 JW0691 Uncharacterized protein ybfBIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN290.135TMH015.1783-330o6-24i45-68o80-101iCytoplasmicMembrane1035.03
YBGE_ECOLIP0AAV0
ybgE
b0735 JW0725 Uncharacterized protein ybgEIntegral Inner Membrane BBpolyout/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN410.437TMH03.17076-330o17-34i46-67o73-96iCytoplasmicMembrane1027.21
FEPA_ECOLIP05825
fepA

fep feuB

fep feuBb0584 JW5086 Ferrienterobactin receptor (Enterobactin outer-membrane receptor) Outer membrane. Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel protein Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544PMID: 12624733 PMID: 11266632 PMID: 9886293 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 10529396 PMID: 25664544Outer Membrane b-barrel proteinHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSKPBAMyesyesY230.836SpI2211.5401-00Yn7-17c22/23oOuterMembrane1011.34
BTUB_ECOLIP06129
btuB

bfe cer dcrC

bfe cer dcrCb3966 JW3938 Uncharacterized protein yhcFCytoplasmic. Outer membrane protein (experimental). Outer membrane. Outer Membrane Outer Membrane Outer Membrane Outer membrane protein (experimental). Outer Membrane ss-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 12652322 PMID: 2982793 PMID: 2687240 PMID: 12595710 PMID: 10806384 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSKPBAMnoyesY210.828SpI2021.8455-00Yn5-16c20/21oOuterMembrane10
FTSQ_ECOLIP06136
ftsQ
b0093 JW0091 Cell division protein ftsQCell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane FtsQ could be localized to the division site in constricting cells. FtsQ insertion requires SecYEG, the transmembrane electrical potential, SecA, and the SRP pathway. PMID: 15546656 YidC plays a role in the membrane integration of the FtsQ protein as the hydrophobic region of FtsQ can be cross-linked to YidC. PMID: 15546656PMID: 2007547 PMID: 9829918 PMID: 9882666 PMID: 11415986 PMID: 11703663 PMID: 11948172 PMID: 15165235 PMID: 17693520 PMID: 15546656 PMID: 15546656Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchoredSRPSECyesyesN410.115TMH01.18618-110i25-49oUnknown224.26
YBGS_ECOLIP0AAV6
ybgS
b0753 JW0736 Uncharacterized protein ybgS Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY250.845SpI2432.7676-00Yn6-17c24/25oPeriplasmic9.84
YBHQ_ECOLIP0AAW5
ybhQ
b0791 JW0774 Inner membrane protein ybhQIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.117TMH07.89577-440i12-32o38-58i79-96o102-120iCytoplasmicMembrane10
YBHT_ECOLIP0AAW9
ybhT
b0762 JW5102 Bacteriophage N4 adsorption protein BSmall membrane protein (experimental). Outer membrane.Experimentally verified orientation: N(out)-C(in). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229Integral Inner Membrane BBoutin/outIM (predicted)Membrane | Single-pass membrane proteinMembrane anchoredSRPYidCnoyesN210.157TMH01.49031-110i7-29oCytoplasmicMembrane9.8228.74
CDH_ECOLIP06282
cdh
b3918 JW3889 CDP-diacylglycerol pyrophosphatase (EC 3.6.1.26) (CDP-diacylglycerol phosphatidylhydrolase) (CDP-diglyceride hydrolase)PeriplasmicGSoluble IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSECyesyesN190.264SpI197.43557-10Yn4-14c19/20oCytoplasmicMembrane1055.48
BTUC_ECOLIP06609
btuC
b1711 JW1701 Vitamin B12 import system permease protein BtuC Integral inner membrane (experimental). Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 11266632 PMID: 12004122 PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN340.278TMH013.5896-99Yn16-29c34/35o58-77i89-109o115-133i145-168o188-206i213-232o238-262i274-297o303-322iCytoplasmicMembrane10
BIRA_ECOLIP06709
birA

bioR dhbB

bioR dhbBb3973 JW3941 Bifunctional protein BirA [Includes: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase (EC 6.3.4.15) (Biotin--protein ligase)]. Deletion of BioR resulted in a 15-fold increase of bio operon transcription in the presence of high biotin levels.Nucleoid (experimental).PMID: 23138451 PMID: 23387333Nucleoid, Integral Inner Membrane N, BIM (predicted)CytoplasmicSRPSECyesyesN260.122CYT0-0.200913-020i123-139o145-162iCytoplasmic1037.71
FHUA_ECOLIP06971
fhuA

tonA

tonAb0150 JW0146 Uncharacterized protein yohNOuter membrane protein (experimental). Outer membrane protein (experimental). Outer membrane. Outer membrane protein (experimental). Outer Membrane ss-barrel protein SecB substrate; Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 3079747 Disulfide bridge (351 362;725 731) Disulfide bond-containing protein PMID: 25664544PMID: 16352602 PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 9856937 PMID: 10806384 PMID: 19953543 PMID: 19953543 PMID: 3079747 PMID: 25664544Outer Membrane b-barrel proteinHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECBSECSURA,SKPBAMyesyesY340.805SpI3320.4987-00Yn16-24c33/34oOuterMembrane107.42
YBIJ_ECOLIP0AAX3
ybiJ
b0802 JW0787 Uncharacterized protein ybiJPeriplasmicGN/APeriplasmPeriplasmicSECyesyesY230.811SpI2221.8351-10Yn7-18c22/23oUnknown2.5
MCBA_ECOLIP0AAX6
mcbA

ybiM

ybiMb0806 JW5106 Uncharacterized protein mcbA (MqsR-controlled colanic acid and biofilm protein A)PeriplasmicGN/APeriplasmPeriplasmicSECyesyesY230.869SpI2218.838-10Yn4-14c22/23oUnknown2.5
YBJH_ECOLIP0AAY4
ybjH
b0843 JW0827 Uncharacterized protein ybjHPeriplasmicGN/APeriplasmicSECyesyesY230.874SpI2225.9172-10Yn6-17c22/23oUnknown2.510.11
FHUB_ECOLIP06972
fhuB
b0153 JW0149 Iron(3+)-hydroxamate import system permease protein fhuB (Ferric hydroxamate uptake protein B) (Ferrichrome transport system permease protein fhuB) (Ferrichrome uptake protein fhuB) (Iron(III)-hydroxamate import system permease protein fhuB)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.415TMH012.8147-1717Yn5-20c38/39o62-79i91-111o117-137i144-167o194-212i224-241o247-265i277-299o305-326i347-369o389-411i423-443o449-468i480-499o567-594i606-623o635-655iCytoplasmicMembrane10
OMPC_ECOLIP06996
ompC

meoA par

meoA parb2215 JW2203 Bacteriophage N4 adsorption protein AOuter membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinOuter membrane protein related to resistance to ampicillin and tetracycline; Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 16372265 PMID: 17908933 PMID: 22658749 PMID: 19737520 PMID: 12488465 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 10806384 PMID: 21541338 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)TFSECSKPBAMyesyesY220.884SpI2121.1188-00Yn5-16c21/22oOuterMembrane106.56
PNTA_ECOLIP07001
pntA
b1603 JW1595 NAD(P) transhydrogenase subunit alpha (EC 1.6.1.2) (Nicotinamide nucleotide transhydrogenase subunit alpha) (Pyridine nucleotide transhydrogenase subunit alpha)Integral inner membrane (experimental). Integral Membrane Protein Integral Membrane Protein Integral Membrane ProteinPMID: 14660605 PMID: 15919996 PMID: 3525165 PMID: 3525165 PMID: 1932078Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN400.111CYT0-0.200913-540i400-418o424-443i455-472o478-499iCytoplasmicMembrane10110.88
YBJO_ECOLIP0AAZ0
ybjO
b0858 JW0842 Inner membrane protein ybjOIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN180.159TMH04.12536-440i21-44o64-87i94-111o131-151iCytoplasmicMembrane1032.79
YCBK_ECOLIP0AB06
ycbK
b0926 JW0909 Uncharacterized protein ycbKTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTATyesyesN310.559SpI308.29508-10Yn11-22c30/31oUnknown2.511.19
YCCF_ECOLIP0AB12
yccF
b0961 JW0944 Inner membrane protein yccFIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN240.116TMH02.25765-330i7-33o69-90i102-123oCytoplasmicMembrane10
MCP2_ECOLIP07017
tar

cheM

cheMb1886 JW1875 Methyl-accepting chemotaxis protein II (MCP-II) (Aspartate chemoreceptor protein)Integral inner membrane (experimental). Integral inner membrane (experimental). Integral Membrane Protein Integral Membrane Protein Integral Membrane ProteinPole-derived membrane fragments were isolated by affinity capture of vesicles containing the chemotaxis protein, Tar. PMID: 3049536 PMID: 12826616 PMID: 12826616 PMID: 17144658PMID: 22380631 PMID: 15919996 PMID: 3049536 PMID: 12826616 PMID: 17144658Integral Inner Membrane BBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN200.158TMH04.99054-21Yn10-21c26/27o192-212iCytoplasmicMembrane1073.56
MCP4_ECOLIP07018
tap
b1885 JW1874 Methyl-accepting chemotaxis protein IV (MCP-IV) (Dipeptide chemoreceptor protein)Cell inner membrane Integral inner membrane (experimental).PMID: 22380631 PMID: 15919996Integral Inner Membrane BBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN350.179TMH06.20402-21Yn10-21c34/35o187-209iCytoplasmicMembrane105.39
YFIB_ECOLIP07021
yfiB
b2605 JW2586 Putative lipoprotein yfiBOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN250.134SpII1818.8761Pos+2=Q00Yn5-16c20/21oOuterMembrane9.9274.52
USHA_ECOLIP07024
ushA
b0480 JW0469 Protein ushA [Includes: UDP-sugar hydrolase (EC 3.6.1.45) (UDP-sugar diphosphatase) (UDP-sugar pyrophosphatase); 5'-nucleotidase (5'-NT) (EC 3.1.3.5)]Periplasmic Disulfide bridge (258 275) Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 25664544PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECnoyesY260.748SpI2511.3752-00Yn7-18c25/26oPeriplasmic1077.35
YCEK_ECOLIP0AB31
yceK
b1050 JW5151 Uncharacterized protein yceKLipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesN310.129SpII155.35698Pos+2=G00Yn3-13c18/19oUnknown2
YCFJ_ECOLIP0AB35
ycfJ
b1110 JW1096 Uncharacterized protein ycfJIntegral Inner Membrane B-N/AMembrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN360.275SpI238.09735-10Yn6-17c21/22oUnknown2.5
BHSA_ECOLIP0AB40
bhsA

comC ycfR

comC ycfRb1112 JW1098 Multiple stress resistance protein BhsAPeripheral outer membrane protein.By membrane fractionation, ComC was shown to be localized in the outer membrane.PMID: 22089859Peripheral outer membrane protein facing the periplasmF3N/APeriplasmPeriplasmicSECyesyesY230.773SpI2221.6297-00Yn6-14c22/23oUnknown2.570.66
PPA_ECOLIP07102
appA
b0980 JW0963 Periplasmic AppA protein [Includes: Phosphoanhydride phosphohydrolase (EC 3.1.3.2) (pH 2.5 acid phosphatase) (AP); 4-phytase (EC 3.1.3.26)] Periplasmic Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 8387749 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBCyesyesY230.793SpI2213.6965-00Yn3-13c22/23oPeriplasmic1066.26
PUTP_ECOLIP07117
putP
b1015 JW1001 Sodium/proline symporter (Proline permease) (Propionate transporter)Cell inner membrane Integral inner membrane (experimental). Integral Membrane ProteinPMID: 16079137 PMID: 15919996 PMID: 3302614Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN280.128TMH013.7134-12120i7-26o66-88i127-152o164-186i193-213o233-256i277-296o325-350i371-391o403-422i429-449o455-473iCytoplasmicMembrane10
PSTA_ECOLIP07654
pstA

phoT

phoTb3726 JW3704 Phosphate transport system permease protein pstAIntegral Inner membrane (experimental). Integral Inner membrane (experimental).PMID: 3881386 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN140.135TMH014.9057-660i29-50o89-111i123-145o151-169i201-222o267-290iCytoplasmicMembrane10
FHUD_ECOLIP07822
fhuD
b0152 JW0148 Iron(3+)-hydroxamate-binding protein fhuD (Ferric hydroxamate uptake protein D) (Ferrichrome-binding periplasmic protein) (Iron(III)-hydroxamate-binding protein fhuD)Periplasmic TAT secretion system. PMID: 8522527 Iron ion binding protein PMID: 17218314 PMID: 8522527 PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECyesyesY310.749SpI308.81274-00Yn12-23c30/31oPeriplasmic10
YMGD_ECOLIP0AB46
ymgD
b1171 JW5177 Uncharacterized protein ymgD Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECnoyesY200.656SpI1914.7848-00Yn4-14c19/20oUnknown2.586.15
YCHH_ECOLIP0AB49
ychH
b1205 JW1196 Uncharacterized protein ychHIntegral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN440.123TMH012.8445-220i12-29o41-59iCytoplasmicMembrane9.8273.18
ATPZ_ECOLIP0ABC0
atpI

uncI

uncIb3739 JW5611 ATP synthase protein IIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN640.113TMH04.01303-43Yn14-25c30/31o40-59i71-94o100-122iCytoplasmicMembrane1034.91
MRED_ECOLIP0ABH4
mreD
b3249 JW3218 Rod shape-determining protein mreDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.118TMH015.556-550o6-25i37-54o74-92i104-128o134-152iCytoplasmicMembrane10
FIMF_ECOLIP08189
fimF
b4318 JW4281 Protein fimFCell surface appendage Fim operon (class γ1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (38 78) Disulfide bond-containing protein PMID: 25664544PMID: 19820722 PMID: 2890081 PMID: 20345943 PMID: 15372038 PMID: 25664544Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY230.871SpI2217.2366-00Yn6-17c22/23oExtracellular9.72
FIMG_ECOLIP08190
fimG
b4319 JW4282 Protein fimGCell surface appendage Fim operon (class γ1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (39 77) Disulfide bond-containing protein PMID: 25664544PMID: 19820722 PMID: 2890081 PMID: 20345943 PMID: 15372038 PMID: 25664544Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY240.773SpI2318.1281-10Yn7-18c23/24oExtracellular9.6573.47
FIMH_ECOLIP08191
fimH
b4320 JW4283 Protein fimHCell surface appendage Cell surface appendage Cell surface appendage Cell surface appendage Fim operon (class γ1); Potential tip-adhesin. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 1971261 PMID: 2900235 PMID: 2890081 PMID: 20345943PMID: 19820722 PMID: 2890081 PMID: 20345943 PMID: 1971261 PMID: 2900235 PMID: 24140205Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY200.539SpI2114.3197-00Yn4-14c19/20oUnknown4.6519.94
GLPT_ECOLIP08194
glpT
b2240 JW2234 Glycerol-3-phosphate transporter (G-3-P transporter) (G-3-P permease) Integral inner membrane (experimental).PMID: 11266632 PMID: 12893935 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN520.128CYT0-0.200913-12120i26-44o64-82i94-113o119-138i159-183o189-208i254-272o292-311i323-342o348-373i385-405o417-437iCytoplasmicMembrane10
CYOD_ECOLIP0ABJ6
cyoD
b0429 JW0419 Cytochrome o ubiquinol oxidase protein cyoD (Ubiquinol oxidase chain D)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN440.15TMH014.0581-330o18-37i44-68o80-100iCytoplasmicMembrane10
CCMD_ECOLIP0ABM5
ccmD

yojM

yojMb2198 JW2186 Heme exporter protein D (Cytochrome c-type biogenesis protein CcmD)Integral Inner Membrane BBinoutout/inIM (predicted)Cell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN70.153TMH03.24028-110o12-37iCytoplasmicMembrane9.8266.64
EXBD_ECOLIP0ABV2
exbD
b3005 JW2973 Biopolymer transport protein exbDIntegral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass type II membrane proteinMembrane anchoredSRPSECyesyesN420.15TMH00.162291-110o20-41iCytoplasmicMembrane9.9747.5
TOLR_ECOLIP0ABV6
tolR
b0738 JW0728 Protein tolRMembrane anchoredPMID: 7744737Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SRPSECyesyesN370.197TMH00.527259-110i21-39oCytoplasmicMembrane9.8223.96
CPDB_ECOLIP08331
cpdB
b4213 JW4171 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC 3.1.3.6) (EC 3.1.4.16)Periplasm Periplasm (experimental). Periplasmic Periplasmic Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 18498364 PMID: 3005231 PMID: 15911532 PMID: 15911532 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSURAnoyesY200.787SpI1914.5002-00Yn4-14c19/20oPeriplasmic1082.97
CRED_ECOLIP08369
creD

cet

cetb4400 JW4363 Inner membrane protein creDIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN290.114TMH04.58166-65Yn11-22c34/35o295-313i325-343o349-368i375-396o402-420iCytoplasmicMembrane1028.1
SPPA_ECOLIP08395
sppA
b1766 JW1755 Protease 4 (EC 3.4.21.-) (Endopeptidase IV) (Protease IV) (Signal peptide peptidase)Cell inner membrane Cell inner membranePMID: 3522590 PMID: 18476724Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN480.115TMH06.31524-110i21-43oCytoplasmicMembrane1014.1
PHOR_ECOLIP08400
phoR

nmpB

nmpBb0400 JW0390 Phosphate regulon sensor protein phoR (EC 2.7.13.3)Integral Inner Membrane Integral inner membrane (experimental).PMID: 2187152 PMID: 15919996Integral Inner Membrane BBpolyin/inN/ACell inner membrane | Multi-pass membrane proteinMembrane anchoredSRPSECyesyesN240.17TMH020.4877-120i12-36o42-59iCytoplasmicMembrane1013.24
FLIL_ECOLIP0ABX8
fliL

cheC1 fla AI fla QI

cheC1 fla AI fla QIb1944 JW1928 Flagellar protein FliLIntegral Inner Membrane BBoutin/outSoluble IM FractionCell inner membrane | Single-pass membrane proteinOuter membrane LipoproteinSRPSECnoyesN430.268SpII2610.5269Pos+2=A110i14-35oCytoplasmicMembrane1035.39
FLIQ_ECOLIP0AC07
fliQ

flaQ

flaQb1949 JW1933 Flagellar biosynthetic protein FliQIntegral Inner Membrane BBinoutin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinBacterial flagellum basal bodyIntegral Membrane ProteinflagellumSRPSECnoyesN410.283TMH014.373-21Yn16-35c40/41o56-74iCytoplasmicMembrane1030.83
CREC_ECOLIP08401
creC

phoM

phoMb4399 JW4362 Sensor protein creC (EC 2.7.13.3)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBinoutout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN250.159SpI27-0.123855-21Yn7-18c24/25o184-206iCytoplasmicMembrane1016.29
DACC_ECOLIP08506
dacC
b0839 JW0823 D-alanyl-D-alanine carboxypeptidase dacC (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Penicillin-binding protein 6) (PBP-6)Peripheral inner membrane protein facing the periplasm. Membrane anchored Peripheral inner membrane protein facing the periplasm.Peripheral inner membrane protein facing the periplasm. PMID: 9858669 DacC(PBP-6) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. PMID: 9371419PMID: 8162192 PMID: 9858669 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell inner membrane | Peripheral membrane proteinMembrane anchored (Experimental)SECyesyesY280.887SpI2718.4084-00Yn11-22c27/28oCytoplasmicMembrane9.9721.34
DSDX_ECOLIP08555
dsdX

yfdA yfdD

yfdA yfdDb2365 JW2362 DsdX permeaseIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN330.118TMH011.5143-12120o6-23i30-47o59-77i98-128o140-158i178-198o225-244i256-282o302-322i343-365o385-408i420-444oCytoplasmicMembrane1012.2
PTV3B_ECOLIP08722
bglF

bglC bglS

bglC bglSb3722 JW3700 PTS system beta-glucoside-specific EIIBCA component (EIIBCA-Bgl) (EII-Bgl) [Includes: Beta-glucoside-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (PTS system beta-glucoside-specific EIIB component); Beta-glucoside permease IIC component Integral inner membrane (experimental).In the absence of β-glucosidase, associates with BglG to form a pro-form membrane complex that maintains BglG inactive.PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.16CYT0-0.200913-10120i100-121o141-160i172-190o210-230i242-263o269-292i299-322o328-347i354-375o381-400i407-424o430-452iCytoplasmicMembrane10
YAAH_ECOLIP0AC98
yaaH
b0010 JW0009 Inner membrane protein yaaHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN340.122TMH09.07966-660i12-31o37-56i63-84o96-116i123-143o149-169iCytoplasmicMembrane10
HOKC_ECOLIP0ACG4
hokC

gef

gefb4412 JW5002 b0018.1Uncharacterized protein yqeIInner membrane.Potentially toxic protein.PMID: 19121005 PMID: 21890895PeriplasmicGN/ACell inner membrane | Single-pass type II membrane proteinPeriplasmicSECnoyesN220.361SpI215.3199-10Yn8-16c21/22oPeriplasmic9.9732.43
HEMX_ECOLIP09127
hemX
b3803 JW3775 Putative uroporphyrinogen-III C-methyltransferase (Urogen-III methylase) (EC 2.1.1.107) (ORF X)Cell inner membranePMID: 16079137Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN500.207CYT0-0.200913-110i36-57oCytoplasmicMembrane10
OMPT_ECOLIP09169
ompT
b0565 JW0554 Protease 7 (EC 3.4.23.49) (Omptin) (Outer membrane protein 3B) (Protease A) (Protease VII)Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinSecB substrate. Trigger factor-sensitive aggregation. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 9298646PMID: 16352602 PMID: 19737520 PMID: 15911532 PMID: 12624733 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 11566868 PMID: 10806384 PMID: 21541338 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECB,TFSECBAMProphage Integrase (EcoGene)nonoY210.295SpI207.49489-00Yn5-16c20/21oOuterMembrane103.86
PTW3C_ECOLIP09323
nagE

pstN

pstNb0679 JW0665 PTS system N-acetylglucosamine-specific EIICBA component (EIICBA-Nag) (EII-Nag) [Includes: N-acetylglucosamine permease IIC component (PTS system N-acetylglucosamine-specific EIIC component); N-acetylglucosamine-specific phosphotransferase enzyme IIB compInner membrane (experimental). Integral inner membrane (experimental).PMID: 15919657 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN450.132TMH03.42438-10120i12-32o38-61i68-88o94-112i132-153o165-185i192-212o232-252i259-276o282-306i313-331o337-359iCytoplasmicMembrane101.97
HOKD_ECOLIP0ACG6
hokD

relF

relFb1562 JW1554 Protein hokD (Protein relF)PeriplasmicGN/ACell membrane | Single-pass membrane proteinPeriplasmicSECProphage Integrase (EcoGene)nonoN230.157SpI222.62207-10Yn8-19c26/27oPeriplasmic9.96
YDBJ_ECOLIP0ACW2
ydbJ
b4529 JW5215 Uncharacterized protein ydbJOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesY250.374SpII1617.2226Pos+2=S00Yn3-14c19/20oUnknown2.524.24
YDCA_ECOLIP0ACW4
ydcA
b1419 JW1416 Uncharacterized protein ydcA Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECnoyesY210.761SpI2012.9463-00Yn4-15c20/21oUnknown2
YEAL_ECOLIP0ACY6
yeaL
b1789 JW1778 UPF0756 membrane protein yeaLIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN250.203TMH08.39191-44Yn4-12c16/17o26-46i53-71o83-100i112-145oCytoplasmicMembrane10
MOTA_ECOLIP09348
motA

flaJ

flaJb1890 JW1879 Motility protein A (Chemotaxis protein MotA)Integral inner membrane (experimental).Interacts with flagellar brake protein YcgR in the flagellar basal bodies or FliM or FliG.PMID: 20303158 PMID: 20346719 PMID: 19364805 PMID: 23193105 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.167TMH04.42407-44Yn2-11c16/17o32-49i70-87o171-189i201-220oCytoplasmicMembrane1018.62
GLPG_ECOLIP09391
glpG
b3424 JW5687 Rhomboid protease glpG (EC 3.4.21.105) (Intramembrane serine protease) Integral inner membrane (experimental). Intramembrane Protease (experimental). Integral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 11266632 PMID: 17051161 PMID: 16216077 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN160.202CYT0-0.200913-660i96-115o135-160i172-188o194-212i224-242o248-267iCytoplasmicMembrane10
GLPQ_ECOLIP09394
glpQ
b2239 JW2233 Glycerophosphoryl diester phosphodiesterase (Glycerophosphodiester phosphodiesterase) (EC 3.1.4.46)Periplasm PeriplasmicPMID: 2829735 PMID: 1851953PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY260.712SpI2517.875-00Yn8-18c25/26oPeriplasmic1039.37
DEDD_ECOLIP09549
dedD
b2314 JW5378 Protein dedD. DedD is a cell division protein; it contains a C-terminal SPOR domain which targets the protein to the septal ring.Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionMembrane anchoredSRPSECyesyesN300.117SpI270.354359-110i9-27oUnknown2
ARGT_ECOLIP09551
argT
b2310 JW2307 Lysine-arginine-ornithine-binding periplasmic protein (LAO-binding protein) Periplasmic Periplasmic This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 2136858 PMID: 19953543 PMID: 9298647 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.595SpI2219.5206-00Yn4-14c22/23oPeriplasmic10
YOHC_ECOLIP0AD17
yohC
b2135 JW5356 Inner membrane protein yohCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN450.135TMH02.03436-550i33-53o65-91i103-122o128-149i170-190oCytoplasmicMembrane1016.06
YOHK_ECOLIP0AD19
yohK
b2142 JW2130 Inner membrane protein yohKIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN240.211TMH01.42711-64Yn7-18c23/24o33-52i91-112o132-162i206-230oCytoplasmicMembrane10
UHPB_ECOLIP09835
uhpB
b3668 JW3643 Sensor protein uhpB (EC 2.7.13.3)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.141TMH016.0266-790o6-28i40-60o80-100i112-132o138-162i183-201o207-224i231-249o255-273iCytoplasmicMembrane1019.08
UHPC_ECOLIP09836
uhpC
b3667 JW3642 Regulatory protein uhpCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN520.143CYT0-0.200913-12120i26-45o65-84i96-114o120-143i155-179o185-203i245-265o285-308i320-338o344-365i377-401o407-431iCytoplasmicMembrane10
UBIB_ECOLIP0A6A0
ubiB

aarF yigQ yigR yigS

aarF yigQ yigR yigSb3835 JW3812 Probable ubiquinone biosynthesis protein ubiBCell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN500.114CYT0-0.200913-220i501-518o524-542iCytoplasmicMembrane1017.98
CLSA_ECOLIP0A6H8
clsA

cls nov yciJ

cls nov yciJb1249 JW1241 Cardiolipin synthase (CL synthase) (EC 2.7.8.-)Cell inner membrane In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. PMID: 22988102PMID: 15919996 PMID: 22988102Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN220.149TMH05.41966-220o6-25i37-58oCytoplasmicMembrane103.69
YFHG_ECOLIP0AD44
yfhG
b2555 JW2539 Uncharacterized protein yfhGTAT secretion system.PMID: 17218314Outer Membrane LipoproteinIN/AOuter membrane LipoproteinTAT,SECLOLyesyesY230.159SpII256.96106Pos+2=V00Yn13-24c29/30oUnknown255.19
YPHA_ECOLIP0AD47
yphA
b2543 JW2527 Inner membrane protein yphAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN580.13TMH013.6817-440i12-33o53-74i81-100o120-137iCytoplasmicMembrane10
OSMB_ECOLIP0ADA7
osmB
b1283 JW1275 Osmotically-inducible lipoprotein BOuter membrane Lipoprotein (experimental).Proven signal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY240.336SpII2322.8573Pos+2=S00Yn9-17c22/23oCytoplasmicMembrane9.86
CYSZ_ECOLIP0A6J3
cysZ
b2413 JW2406 Protein cysZIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 15919996PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPYidCyesyesN180.111TMH05.82324-450i31-51o71-92i143-163o169-188i209-242oCytoplasmicMembrane1019.52
DSBB_ECOLIP0A6M2
dsbB

roxB ycgA

roxB ycgAb1185 JW5182 Disulfide bond formation protein B (Disulfide oxidoreductase)Integral inner membrane (experimental). Alpha-helcal transmembrane protein (experimental). Integral inner membrane (experimental). Integral inner membrane (experimental). Exhibites aberrant localization by microscopy in the absence of YidC PMID: 22108384. The crystal structure revealed a transmembrane (TM) four-helix-bundle structure of DsbBPMID: 18406599. DsbB oxidizes the periplasmic protein DsbA, which in turn oxidizes the cysteines in other periplasmic proteins to make disulfide bondsPMID: 8430071. Gene fusion approaches were used to show that (i) DsbB is a membrane protein which spans the membrane four times and (ii) both the N- and C-termini of the protein are in the cytoplasmPMID: 7957076. The structure of DsbB revealed four transmembrane helices and one short horizontal helix juxtaposed with Cys130 in the mobile periplasmic loopPMID: 17110337. Disulfide bridge (41 44;104 130) PMID: 10064586PMID: 18406599 PMID: 11266632 PMID: 17110337 PMID: 22108384 PMID: 8430071 PMID: 7957076 PMID: 15919996 PMID: 10064586Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN250.286TMH04.8983-440i12-33o45-64i71-89o145-166iCytoplasmicMembrane1015.79
ENO_ECOLIP0A6P9
eno
b2779 JW2750 Enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)Peripheral inner membrane protein facing the cytoplasm (potential). Cytoplasmic Excreted. Cytoskeleton. Cytoplasmic Cytoplasmic Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. PMID: 8610017 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 17242352 PMID: 8610017 PMID: 15003462 PMID: 18337249 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceA, F1, F4Soluble IM FractionCytoplasm -> cytoskeleton | Secreted | Cell surfaceCytoplasmic (Experimental)Uknown mechanismyesyesN420.139CYT0-0.200913-000iCytoplasmic9.97100.97
ECNA_ECOLIP0ADB4
ecnA
b4410 JW5737 Uncharacterized protein yfeNOuter membrane Lipoprotein (experimental).Small membrane protein detected experimentally. Proven pignal peptidase II substrate. PMID: 15174130PMID: 19121005 PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY230.254SpII1814.5257Pos+2=N00Yn5-16c21/22oCytoplasmicMembrane10
YJJB_ECOLIP0ADD2
yjjB
b4363 JW4327 UPF0442 protein yjjB (Protein P-14)Integral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.153TMH07.02974-350o6-27i34-52o58-75i87-109o129-151iCytoplasmicMembrane1011.1
YHHN_ECOLIP0ADI9
yhhN
b3468 JW3433 Uncharacterized membrane protein yhhNIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN190.165TMH07.64349-66Yn3-14c18/19o34-64i76-96o102-123i130-152o158-177i189-207oCytoplasmicMembrane10
FLGH_ECOLIP0A6S0
flgH

fla FVIII flaY

fla FVIII flaYb1079 JW5153 Flagellar L-ring protein (Basal body L-ring protein)Outer membrane Lipoprotein (experimental). Outer membrane protein (experimental). Proven pignal peptidase II substrate. L ring protein. PMID: 15174130PMID: 15174130 PMID: 12631703 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorBacterial flagellum basal bodyOuter membrane Lipoprotein (Experimental)flagellumSECLOLyesyesY240.235SpII2117.0713Pos+2=A00Yn14-25c33/34oOuterMembrane1024.76
FLGI_ECOLIP0A6S3
flgI

fla FIX flaM

fla FIX flaMb1080 JW1067 Flagellar P-ring protein (Basal body P-ring protein) Periplasmic P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 14726535 PMID: 12631703 PMID: 15838024 PMID: 19953543 PMID: 12730325 PMID: 15838024PeriplasmicGN/APeriplasmBacterial flagellum basal bodyPeriplasmic (Experimental)flagellumSRP (exp)SECDSBAFlanoyesY200.783SpI1915.6262-00Yn4-14c19/20oPeriplasmic1063.67
MRAY_ECOLIP0A6W3
mraY

murX

murXb0087 JW0085 Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) (UDP-MurNAc-pentapeptide phosphotransferase)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.151CYT0-0.200913-10100o20-40i73-90o96-114i134-152o172-193i200-219o239-256i263-284o290-311i338-357oCytoplasmicMembrane10
KDGT_ECOLIP0A712
kdgT
b3909 JW5560 2-keto-3-deoxygluconate permease (KDG permease)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN670.134TMH012.8678-10100i12-29o35-57i78-97o103-127i139-164o170-187i194-211o223-243i255-274o286-309iCytoplasmicMembrane10
MSCL_ECOLIP0A742
mscL

yhdC

yhdCb3291 JW3252 Large-conductance mechanosensitive channelCell inner membrane Integral inner membrane (experimental).PMID: 17113597 PMID: 22108384 PMID: 8890153 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPYidCyesyesN390.143CYT0-0.200913-220i21-52o72-96iCytoplasmicMembrane1066.67
YHJT_ECOLIP0ADJ5
yhjT

yhjT

yhjTb3537 JW5663 Uncharacterized protein yhjTIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECnoyesN260.211CYT0-0.200913-110o6-25iUnknown268.44
YIAA_ECOLIP0ADJ8
yiaA
b3562 JW3534 Inner membrane protein yiaAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN220.118TMH07.26426-440i12-30o42-62i74-94o106-125iCytoplasmicMembrane1059.15
YIAW_ECOLIP0ADK4
yiaW
b3587 JW3559 Inner membrane protein yiaWIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.107TMH011.1792-220o6-25i46-66oCytoplasmicMembrane1020.57
MNTH_ECOLIP0A769
mntH

yfeP

yfePb2392 JW2388 Manganese transport protein mntHCell inner membrane Integral inner membrane (experimental).PMID: 14607838 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN280.129TMH09.54648-11110i21-40o52-75i95-116o122-144i156-176o196-216i237-263o283-313i325-343o349-370i391-410oCytoplasmicMembrane10
GPMB_ECOLIP0A7A2
gpmB

ytjC

ytjCb4395 JW4358 Probable phosphoglycerate mutase gpmB (EC 5.4.2.1) (PGAM) (Phosphoglyceromutase)Large potential TM 146-168aaIntegral Inner Membrane BBinoutout/inN/AMembrane anchoredSRPSECnoyesN170.117CYT0-0.200913-110o146-168iCytoplasmic9.9756.14
DCTA_ECOLIP0A830
dctA
b3528 JW3496 Aerobic C4-dicarboxylate transport proteinIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN190.136TMH05.74612-880i9-27o47-64i76-99o141-164i184-208o220-242i326-344o350-373iCytoplasmicMembrane10
TOLB_ECOLIP0A855
tolB
b0740 JW5100 Protein tolBPeriplasm Periplasmic SecB substrate. Might be is co-translationally translocated through Sec translocon. PMID: 17233825 PMID: 7929011 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 16352602 PMID: 15838024 PMID: 7929011 PMID: 17233825 PMID: 15838024PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECB, SRPSECnoyesN220.555SpI217.66433-00Yn7-16c21/22oPeriplasmic1031.59
YICN_ECOLIP0ADL3
yicN
b3663 JW5637 Uncharacterized protein yicNEcholocation says Membrane anchored, one or two TMH predicted, if signal peptide is also predicted these TMH(s) are not removed as it is probably not cleaved.Integral Inner Membrane B-N/AMembrane anchoredSRPSECyesyesN270.132CYT0-0.200913-10Yn6-17c21/22oUnknown223.5
YIDG_ECOLIP0ADL6
yidG
b3675 JW3651 Inner membrane protein yidGIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN470.125TMH05.45771-330i21-39o45-65i91-116oCytoplasmicMembrane1021.31
YIDH_ECOLIP0ADM0
yidH
b3676 JW3652 Inner membrane protein yidHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.135TMH01.23486-330i21-39o54-72i93-114oCytoplasmicMembrane1020.4
YIDQ_ECOLIP0ADM4
yidQ
b3688 JW5633 Uncharacterized protein yidQOuter membrane LipoproteinPMID: 11274153Inner Membrane LipoproteinEN/AOuter membrane Lipoprotein(experimental)SECLOLyesyesY270.185SpII2016.396Pos+2=S00Yn5-18c26/27oUnknown2114.11
ZUPT_ECOLIP0A8H3
zupT

ygiE

ygiEb3040 JW3008 Zinc transporter zupTIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN590.198TMH010.378-670o6-25i32-50o62-81i125-150o170-192i199-215o235-256iCytoplasmicMembrane1027.87
YIHY_ECOLIP0A8K8
yihY
b3886 JW3857 b2268UPF0761 membrane protein yihYCell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinCytoplasmicSRPSECyesyesN600.127CYT0-0.200913-670o39-61i73-91o103-120i141-160o180-200i212-234o240-266iCytoplasmicMembrane10
FRDC_ECOLIP0A8Q0
frdC
b4152 JW4113 Fumarate reductase subunit C (Fumarate reductase 15 kDa hydrophobic protein) Integral inner membrane (experimental). Integral inner membrane (experimental).Alpha-helcal transmembrane protein (experimental). Transmembrane helices: 3. N-terminal region is in cytoplasm. Helix A and B connected by very short turns to an interfacial helix (N51-L63)PMID: 24057063.PMID: 11266632 PMID: 10373108 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN590.224TMH01.78374-330i26-48o68-88i109-130oCytoplasmicMembrane109.34
FRDD_ECOLIP0A8Q3
frdD
b4151 JW4112 Fumarate reductase subunit D (Fumarate reductase 13 kDa hydrophobic protein) Integral inner membrane (experimental). Integral inner membrane (experimental).Alpha-helcal transmembrane protein (experimental). Transmembrane helices: 3 PMID: 10373108.PMID: 11266632 PMID: 10373108 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN520.162TMH015.5267-330i12-45o57-78i99-118oCytoplasmicMembrane107.09
YCJF_ECOLIP0A8R7
ycjF
b1322 JW1315 UPF0283 membrane protein ycjFIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.1CYT0-0.200913-340i70-87o99-120i214-236o256-275iCytoplasmicMembrane1010.64
YIDX_ECOLIP0ADM6
yidX
b3696 JW5858 Uncharacterized protein yidXOuter Membrane LipoproteinIN/AMembrane | Single-pass membrane proteinOuter membrane LipoproteinSECLOLpseudogene (Ochman & Davalos, 2006)noyesY290.411SpII2320.6632Pos+2=I10Yn11-21c26/27oUnknown254
YIFL_ECOLIP0ADN6
yifL
b4558 JW3781 Uncharacterized lipoprotein yifLOuter membrane protein (experimental). Membrane LipoproteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 16397293PMID: 15911532 PMID: 16397293Outer Membrane LipoproteinISoluble IM FractionCell membrane | Lipid-anchorMembrane Lipoprotein (Experimental)SECLOLnoyesY240.159SpII1927.2584Pos+2=G10Yn7-18c23/24oUnknown2
YGAM_ECOLIP0ADQ7
ygaM
b2672 JW2647 Uncharacterized protein ygaMInner membrane, ribosomal binding YgaM is paralogous to YqjD and binds to ribsomes in a similar manner to YqjD. PMID: 22661687PMID: 22661687Integral Inner Membrane BBinin/outIM (predicted), Detected in IM FractionMembrane anchoredSRPSECnoyesN400.127CYT0-0.200913-110i87-106oUnknown250.39
YCEI_ECOLIP0A8X2
yceI
b1056 JW1043 Protein yceIPeriplasmic Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.827SpI2223.1728-00Yn4-15c22/23oUnknown2.569.38
BLC_ECOLIP0A901
blc

yjeL

yjeLb4149 JW4110 Outer membrane lipoprotein blcOuter membrane Lipoprotein (experimental). Outer membrane protein (experimental). Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130PMID: 15174130 PMID: 20932056 PMID: 21541338Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY190.216SpII1814.624Pos+2=S00Yn3-15c20/21oOuterMembrane1052.35
BAMC_ECOLIP0A903
nlpB

dapX nlpB

dapX nlpBb2477 JW2462 Lipoprotein 34Lipoprotein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Cell outer membrane Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein related to resistance to ampicillin and tetracycline. Part of bam complex. Forms subcomplex with BamCE. Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384PMID: 16372265 PMID: 21823654 PMID: 21139201 PMID: 21370981 PMID: 15174130 PMID: 12488465 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 16079137 PMID: 20932056 PMID: 10806384 PMID: 21541338Outer Membrane LipoproteinISoluble IM FractionCell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN320.177SpII2417.2458Pos+2=S00Yn13-23c27/28oOuterMembrane1086.64
SLYB_ECOLIP0A905
slyB
b1641 JW1633 Outer membrane lipoprotein slyBLipoprotein (experimental). Inner membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384PMID: 15174130 PMID: 15911532 PMID: 10806384 PMID: 20932056 PMID: 10806384 PMID: 21541338Outer Membrane LipoproteinISoluble IM FractionCell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN220.181SpII1719.9051Pos+2=V00Yn5-16c21/22oOuterMembrane9.92
YGDD_ECOLIP0ADR2
ygdD
b2807 JW2778 UPF0382 inner membrane protein ygdDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN250.249TMH014.4685-43Yn7-20c24/25o40-61i68-91o97-121iCytoplasmicMembrane10
YGGE_ECOLIP0ADS6
yggE
b2922 JW2889 Uncharacterized protein yggEPeriplasmicGN/APeriplasmicSECyesyesY220.409SpI2116.0009-00Yn5-16c21/22oPeriplasmic9.76111.24
YGIW_ECOLIP0ADU5
ygiW
b3024 JW2992 Protein ygiWPeriplasmicSecB substrate. PMID: 9298646PMID: 16352602 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECBSECyesyesY210.877SpI2026.1756-00Yn4-15c20/21oUnknown2.528.78
MIPA_ECOLIP0A908
mipA

yeaF

yeaFb1782 JW1771 MltA-interacting proteinOuter Membrane ss-barrel protein Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental).MipA is considered to be a structural protein mediating the assembly of MltA to PBP1B into a complexPMID: 10037771. MipA couples both enzymes to one another in vivo, it is likely to give rise to a membrane adhesion site as depicted. Such contacts between the membranes. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000,Thein 2010)PMID: 15911532PMID: 10806384PMID: 12624733PMID: 20932056 PMID: 10037771PMID: 10037771 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 16079137 PMID: 20932056Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECBAMyesyesY230.835SpI2221.2923-00Yn6-17c22/23oOuterMembrane1011.35
OMPA_ECOLIP0A910
ompA

con tolG tut

con tolG tutb0957 JW0940 Outer membrane protein A (Outer membrane protein II*)Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane Outer Membrane Outer Membrane Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel protein Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544PMID: 8626309 PMID: 17908933 PMID: 19343722 PMID: 19737520 PMID: 23641708 PMID: 12488465 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 9808047 PMID: 7813480 PMID: 16079137 PMID: 21778229 PMID: 20932056 PMID: 10806384 PMID: 21541338 PMID: 19953543 PMID: 19953543 PMID: 19953543 PMID: 9298646 PMID: 24717922 PMID: 25664544Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)TFSECSKP,SURA,DEGP,DSBABAMyesyesY220.872SpI2128.1613-00Yn5-16c21/22oOuterMembrane1010.56
PAL_ECOLIP0A912
pal

excC

excCb0741 JW0731 Peptidoglycan-associated lipoproteinLipoprotein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane Lipoprotein Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 10806384 PMID: 9298646PMID: 15174130 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 19364805 PMID: 23193105 PMID: 20932056 PMID: 10806384 PMID: 9298646Outer Membrane LipoproteinISoluble IM FractionCell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY290.5SpII2121.6668Pos+2=S00Yn6-14c18/19oOuterMembrane1081.44
LPTA_ECOLIP0ADV1
lptA

yhbN

yhbNb3200 JW3167 Lipopolysaccharide export system protein lptAPeriplasmic. Periplasm PeriplasmicPMID: 17056748 PMID: 18480051 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic(experimental)SECyesyesY280.838SpI2724.4302-00Yn12-23c27/28oUnknown2.523.4
MLAC_ECOLIP0ADV7
mlaC

yrbC

yrbCb3192 JW3159 Probable phospholipid-binding protein mlaC PeriplasmicPMID: 15911532PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY220.509SpI2114.0896-00Yn8-16c21/22oUnknown2.561.74
OMPW_ECOLIP0A915
ompW

yciD

yciDb1256 JW1248 Outer membrane protein WOuter membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinOuter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 9298646PMID: 16372265 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 16414958 PMID: 20932056 PMID: 10806384 PMID: 9298646Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECBAMyesyesY220.754SpI2126.4914-00Yn4-15c21/22oOuterMembrane1042.93
OMPX_ECOLIP0A917
ompX

ybiG

ybiGb0814 JW0799 Outer membrane protein XOuter membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinSecB substrate. Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 9298646PMID: 16352602 PMID: 19343722 PMID: 22658749 PMID: 19737520 PMID: 12488465 PMID: 15911532 PMID: 10806384 PMID: 11266632 PMID: 10545325 PMID: 20932056 PMID: 10806384 PMID: 21541338 PMID: 19953543 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECB,TFSECSURA,SKPBAMyesyesY240.63SpI2326.0753-10Yn4-15c23/24oOuterMembrane1014.31
PA1_ECOLIP0A921
pldA
b3821 JW3794 Phospholipase A1 (EC 3.1.1.32) (EC 3.1.1.4) (Detergent-resistant phospholipase A) (DR-phospholipase A) (Outer membrane phospholipase A) (OM PLA) (OMPLA) (Phosphatidylcholine 1-acylhydrolase) Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 PMID: 6397464PMID: 12624733 PMID: 11266632 PMID: 10537112 PMID: 6397463 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 6397464Outer Membrane b-barrel proteinHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSKPBAMyesyesY210.528SpI207.74532-00Yn3-14c20/21oOuterMembrane109.69
YHHA_ECOLIP0ADX7
yhhA
b3448 JW3413 Uncharacterized protein yhhA (ORFQ)PeriplasmicGN/APeriplasmicSECyesyesY180.377SpI1714.5283-00Yn4-11c17/18oUnknown2.5107.69
YQAE_ECOLIP0AE42
yqaE
b2666 JW2641 UPF0057 membrane protein yqaEIntegral Inner Membrane BBinoutout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN270.14TMH06.73627-210o23-48iCytoplasmicMembrane10
CREA_ECOLIP0AE91
creA

yjjD

yjjDb4397 JW4360 Protein creA Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 25664544PeriplasmicGN/APeriplasmicSECDSBAyesyesY220.8SpI2114.9398-00Yn6-16c21/22oUnknown2.5
YJIG_ECOLIP0AEH8
yjiG
b4329 JW4292 Inner membrane protein yjiGIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN360.157TMH02.83449-340i21-39o59-77i89-110o130-152iCytoplasmicMembrane102.44
PGPB_ECOLIP0A924
pgpB
b1278 JW1270 Phosphatidylglycerophosphatase B (EC 3.1.3.27)Cell inner membrane, Cell outer membrane Integral inner membrane (experimental). Cell inner membrane, Cell outer membranePgpB protein has phosphatidyl glycerophosphate phosphatase activity higher in the cytoplasmic membrane, while it has phosphatidic acid and lysophosphatidic acid phosphatase activities higher in the outer membrane.PMID: 2846511 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinCell outer membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN240.144TMH017.4928-660i12-29o49-68i75-96o157-178i185-206o212-230iCytoplasmicMembrane10
TSX_ECOLIP0A927
tsx

nupA

nupAb0411 JW0401 Nucleoside-specific channel-forming protein tsxOuter membrane protein (experimental). Outer Membrane β-barrel protein (experimental). Outer membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384PMID: 16372265 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 19953543Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECBAMProphage TransposaseyesnoY230.882SpI2224.8185-00Yn4-14c22/23oOuterMembrane100
WZA_ECOLIP0A930
wza
b2062 JW2047 Putative polysaccharide export protein wzaOuter membrane, Lipoprotein. Lipoprotein (experimental). Surface exposed.Has a helical structure that crosses membrane. The transmembrane region of Wza is an alpha-helical barrel. Proven pignal peptidase II substrate. It has been demostrated that it can be also secreted and be surface exposed. PMID: 15174130PMCID: PMC3315050 PMID: 15174130 PMID: 10619844Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceI, F4N/ACell outer membrane | Multi-pass membrane proteinOuter membrane Lipoprotein (Experimental)SECLOLyesyesY260.13SpII2012.3938Pos+2=T00Yn11-21c25/26oOuterMembrane9.9244.16
GFCE_ECOLIP0A932
gfcE

yccZ

yccZb0983 JW0966 Putative polysaccharide export protein gfcE (Group 4 capsule protein E homolog)Lipoprotein (experimental). Outer membrane protein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane | Multi-pass membrane proteinOuter membrane Lipoprotein (Experimental)SECLOLyesyesY230.202SpII2015.093Pos+2=T00Yn10-21c26/27oOuterMembrane9.9259.67
YIIR_ECOLIP0AF34
yiiR
b3921 JW3892 Uncharacterized protein yiiRIntegral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN380.144TMH05.06913-440i20-38o44-63i75-93o99-121iCytoplasmicMembrane1049.81
YIJD_ECOLIP0AF40
yijD
b3964 JW3936 Inner membrane protein yijDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.345TMH08.09717-43Yn10-21c26/27o36-51i63-83o89-108iCytoplasmicMembrane1021.82
YJBE_ECOLIP0AF45
yjbE
b4026 JW3986 Uncharacterized protein yjbEPeriplasmicGN/APeriplasmicSECnoyesY220.853SpI2125.8362-00Yn4-15c21/22oExtracellular9.65
MLTA_ECOLIP0A935
mltA

mlt ygdM

mlt ygdMb2813 JW2784 Membrane-bound lytic murein transglycosylase A (EC 4.2.2.n1) (Mlt38) (Murein hydrolase A)Lipoprotein (experimental). Outer membrane protein (experimental). The formation of a complex between a murein polymerase (PBP1B) and a murein hydrolase (MltA). In the presence of MipA represents a first step in a reconstitution of the hypothetical murein-synthesizing holoenzyme. Postulated to be responsible for controlled growth of the stress-bearing sacculus of E.coli. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) PMID: 15174130 SRP recognizes the first signal anchor sequence of a polytopic membrane protein (MtlA) resulting in cotranslational targeting of MtlA to SecY and phospholipids of the plasma membrane. PMID: 10619852 Possible interaction of SRP with the secretory protein pOmpA is prevented by the association of trigger factor with nascent pOmpA. PMID: 10619852PMID: 15174130 PMID: 10037771 PMID: 12624733 PMID: 20932056 PMID: 10619852 PMID: 10619852Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLpseudogene (Ochman & Davalos, 2006)noyesY200.191SpII2022.7365Pos+2=S00Yn9-19c23/24oOuterMembrane1035.58
BAME_ECOLIP0A937
smpA

smpA

smpAb2617 JW2598 Small protein ACell outer membrane Outer membrane protein (experimental).Part of BAM complex. Forms subcomplex with BamCE.PMID: 21823654 PMID: 21139201 PMID: 21370981 PMID: 17404237 PMID: 20932056Outer Membrane LipoproteinISoluble IM FractionCell outer membrane | Lipid-anchorOuter membrane LipoproteinSECLOLyesyesY240.197SpII1919.5033Pos+2=S00Yn5-16c20/21oOuterMembrane9.9244.38
BAMA_ECOLIP0A940
bamA

yaeT yzzN yzzY

yaeT yzzN yzzYb0177 JW0172 Outer membrane protein assembly factor yaeT (Omp85)Outer membrane protein (experimental). Outer Membrane Outer membrane protein (experimental). Outer Membrane ss-barrel proteinPart of bam complexPMID: 21823654PMID: 21370981. Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamAPMID: 23641708. Outer membrane protein determined by 2D gel electrophoresis (PMID: 15911532,PMID: 12624733,PMID: 20932056) Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 21823654 PMID: 21370981 PMID: 19525348 PMID: 23641708 PMID: 12624733 PMID: 10806384 PMID: 16079137 PMID: 20932056 PMID: 19953543 PMID: 9298646Outer Membrane b-barrel proteinHSoluble IM FractionCell outer membraneOuter Membrane ss-barrel protein(experimental)SECBAMyesyesY210.641SpI2010.5737-00Yn6-13c20/21oOuterMembrane1032.48
YJCH_ECOLIP0AF54
yjcH
b4068 JW4029 Inner membrane protein yjcHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN480.119TMH04.65174-220i25-51o63-84iCytoplasmicMembrane1054.4
YJCO_ECOLIP0AF56
yjcO
b4078 JW4039 Uncharacterized protein yjcO Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY180.752SpI1713.9637-00Yn4-13c17/18oUnknown2.582.96
YJET_ECOLIP0AF73
yjeT
b4176 JW4134 Uncharacterized protein yjeTIntegral Inner Membrane BBinoutin/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN270.108TMH04.78812-21Yn3-13c17/18o41-60iCytoplasmicMembrane9.8219.85
PANE_ECOLIP0A9J4
panE

apbA

apbAb0425 JW0415 2-dehydropantoate 2-reductase (EC 1.1.1.169) (Ketopantoate reductase) (KPA reductase) (KPR)Cytoplasmic Lipoprotein CytoplasmicPhobious predicts the sequence that follows signal peptide as non cytoplasmic and non transmembrane. The native enzyme exists in solution as a monomer with a molecular mass of 34 000 Da. PMID: 10736170 Lipoprotein PMID: 10572132 PMID: 15174130 PMID: 10736170PMID: 10736170 PMID: 10572132 PMID: 15174130Outer Membrane LipoproteinIIM (predicted)CytoplasmCytoplasmic (Experimental)SECLOLyesyesN210.133SpII192.5798Pos+2=K00Yn5-16c20/21oCytoplasmic8.9631.23
PTA_ECOLIP0A9M8
pta
b2297 JW2294 Phosphate acetyltransferase (EC 2.3.1.8) (Phosphotransacetylase)Trigger factor-sensitive aggregation. Deletion of the ackA gene alone or together with Pta showed acetic acid accumulation. The deletion mutants of the gene encoding phosphotransacetylase secrete pyruvate before the secretion of acetate.PMID: 19737520 PMID: 21796982 PMID: 23194590 PMID: 20703804 PMID: 7765717PeriplasmicGIM (predicted-detected)CytoplasmCytoplasmicSRP,TFSECnoyesN670.109SpI221.30744-00Yn7-17c22/23oCytoplasmic9.2654.45
TRXB_ECOLIP0A9P4
trxB
b0888 JW0871 Thioredoxin reductase (TRXR) (EC 1.8.1.9) Disulfide bridge (136 139) PMID: 8114095PMID: 8114095PeriplasmicGN/ACytoplasmCytoplasmicSECyesyesN260.317SpI256.69011-00Yn9-17c25/26oCytoplasmic10104.59
PLAP_ECOLIP0AA47
plaP

yeeF

yeeFb2014 JW5330 Inner membrane transport protein yeeFIntegral inner membrane (experimental).Old gene name YeeF.PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.148TMH08.96423-12120i20-42o54-73i94-116o128-146i158-179o199-217i237-260o287-315i336-355o361-383i395-413o419-437iCytoplasmicMembrane10
YJFL_ECOLIP0AF80
yjfL
b4184 JW4142 UPF0719 inner membrane protein yjfLIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.123TMH08.65955-440o6-28i49-69o75-97i109-128oCytoplasmicMembrane1019.01
YJFN_ECOLIP0AF82
yjfN
b4188 JW5742 Uncharacterized protein yjfNPeriplasmicGN/APeriplasmPeriplasmicSECyesyesN200.293SpI217.51366-00Yn4-15c23/24oUnknown2104.21
YJFY_ECOLIP0AF86
yjfY
b4199 JW4157 Uncharacterized protein yjfYPeriplasmicGN/APeriplasmPeriplasmicSECyesyesY210.833SpI2015.6931-00Yn5-15c20/21oUnknown2.593.7
YFDV_ECOLIP0AA49
yfdV
b2372 JW2369 Uncharacterized transporter yfdVIntegral Membrane ProteinPMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN570.126CYT0-0.200913-10100o6-23i35-55o67-90i97-117o129-150i175-196o202-222i234-253o259-278i290-309oCytoplasmicMembrane10
QMCA_ECOLIP0AA53
qmcA

ybbK

ybbKb0489 JW0478 Protein QmcACell inner membranePMID: 16573693Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionCell inner membrane | Single-pass type I membrane proteinMembrane anchoredSRPSECyesyesN180.11TMH01.35378-10Yn2-13c18/19oUnknown213.53
YGHB_ECOLIP0AA60
yghB
b3009 JW2976 Inner membrane protein yghBIntegral inner membrane (experimental).Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF.PMID: 15919996 PMID: 23086209Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.338TMH00.0786812-460o20-38i45-62o68-88i123-143o155-176i188-212oCytoplasmicMembrane108.12
YQJA_ECOLIP0AA63
yqjA
b3095 JW3066 Inner membrane protein yqjAIntegral inner membrane (experimental).Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF.PMID: 15919996 PMID: 23086209Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein SRPSECyesyesN210.127TMH04.47784-660o20-38i45-62o68-88i123-143o155-176i188-212oCytoplasmicMembrane103.55
RHTA_ECOLIP0AA67
rhtA

ybiF

ybiFb0813 JW0798 Inner membrane transporter rhtACell inner membrane Integral inner membrane (experimental).PMID: 12648727 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN320.17TMH06.46418-109Yn10-18c22/23o38-59i71-89o95-115i122-139o145-165i177-196o202-225i237-258o264-283iCytoplasmicMembrane10
PSIF_ECOLIP0AFM4
psiF
b0384 JW5054 Phosphate starvation-inducible protein psiF Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY220.376SpI2113.709-10Yn5-16c21/22oUnknown244.48
PSPB_ECOLIP0AFM9
pspB
b1305 JW1298 Uncharacterized protein yqeK Defects in yidC and sec secretion induce psp operon. PMID: 14594846PMID: 14594846Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECProphage Transposase (by homology to BL21)noyesN300.112CYT0-0.200913-110o6-24iCytoplasmicMembrane1062.96
PSPC_ECOLIP0AFN2
pspC
b1306 JW1299 Phage shock protein C Defects in yidC and sec secretion induce psp operon. PMID: 14594846PMID: 14594846Integral Inner Membrane BBinoutout/inIM (predicted)Cell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECProphage TransposaseyesnoN250.142CYT0-0.200913-110o39-64iCytoplasmicMembrane9.8229.03
YADS_ECOLIP0AFP0
yadS
b0157 JW0153 UPF0126 inner membrane protein yadSIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.24TMH02.68818-770o6-23i30-50o62-79i91-110o116-137i149-168o174-190iCytoplasmicMembrane10
YEDA_ECOLIP0AA70
yedA
b1959 JW1942 Uncharacterized inner membrane transporter yedAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.125TMH011.0617-109Yn6-16c21/22o37-56i68-86o98-117i124-143o149-169i176-199o211-233i245-262o268-290iCytoplasmicMembrane10
YHBE_ECOLIP0AA73
yhbE
b3184 JW3151 Uncharacterized inner membrane transporter yhbEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.149TMH00.144382-10100i7-26o38-56i71-91o103-120i132-151o157-177i189-205o217-236i248-269o281-299iCytoplasmicMembrane10
DGOT_ECOLIP0AA76
dgoT

yidT

yidTb3691 JW5859 D-galactonate transporterIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN430.138TMH04.18214-11120i15-35o55-75i82-98o104-122i142-163o169-188i242-263o283-304i316-332o338-362i369-392o404-424iCytoplasmicMembrane10
EXUT_ECOLIP0AA78
exuT
b3093 JW3064 Hexuronate transporterIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN180.107TMH00.456812-11120i47-66o86-109i116-136o142-162i174-199o205-224i277-299o305-325i345-362o368-389i401-424o430-452iCytoplasmicMembrane10
GARP_ECOLIP0AA80
garP

yhaU

yhaUb3127 JW3096 Probable galactarate transporter (D-galactarate permease)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN330.195TMH03.99251-11120i12-32o52-73i85-103o109-130i151-173o179-199i251-270o290-314i326-345o351-372i379-406o412-434iCytoplasmicMembrane10
RUTC_ECOLIP0AFQ5
rutC

ycdK

ycdKb1010 JW0995 UPF0076 protein rutC (Pyrimidine utilization protein C)PeriplasmicGN/APeriplasmicSECnoyesN460.249SpI242.38691-00Yn5-16c24/25oCytoplasmic9.2638.29
YHID_ECOLIP0AFV2
yhiD

yhhE

yhhEb3508 JW5670 Uncharacterized protein yhiDIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN550.254CYT0-0.200913-550o6-22i34-54o69-87i92-112o118-138iCytoplasmicMembrane1028.82
YMIA_ECOLIP0CB62
ymiA
b4522 JW1267.1 Uncharacterized protein ymiASmall membrane protein detected experimentallyPMID: 19121005Integral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane protein-SECnoyesN350.102TMH01.19842-110i20-41oCytoplasmicMembrane9.82
CODB_ECOLIP0AA82
codB
b0336 JW0327 Cytosine permeaseIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN380.154TMH012.7134-12120i20-40o52-74i95-118o130-147i152-173o193-212i224-253o259-278i299-318o324-345i357-379o385-402iCytoplasmicMembrane10
DSBE_ECOLIP0AA86
dsbE

ccmG yejQ

ccmG yejQb2195 JW2183 Thiol:disulfide interchange protein dsbE (Cytochrome c biogenesis protein CcmG)Peripheral inner membrane protein facing the periplasm (experimental). Periplasmic with N-terminal membrane anchor. Periplasmic with N-terminal membrane anchor The CcmG protein was shown to be membrane bound, facing the periplasm with its C-terminal, hydrophilic domain. PMID: 9537397 PMID: 16364305 Disulfide bridge (80 83) PMID: 9537397 PMID: 16364305 Integral Inner Membrane BBoutin/outN/ACell inner membrane | Single-pass membrane protein | Periplasmic sidePeriplasmic with N-terminal membrane anchor (Experimental)SRPSECyesyesN290.264SpI278.59712-10Yn6-21c30/31oCytoplasmicMembrane1069.04
YEAY_ECOLIP0AA91
yeaY
b1806 JW1795 Uncharacterized lipoprotein yeaYLipoprotein (experimental). Outer membrane protein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN270.138SpII2214.0614Pos+2=V00Yn10-20c25/26oOuterMembrane9.9225.94
YPDA_ECOLIP0AA93
ypdA
b2380 JW5388 Inner membrane protein ypdAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN160.103TMH01.16348-660o6-25i45-64o76-99i106-128o140-161i173-191oCytoplasmicMembrane10
YIAB_ECOLIP11286
yiaB
b3563 JW5654 Inner membrane protein yiaBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN500.132TMH07.23112-440i7-26o32-50i62-87o93-110iCytoplasmicMembrane1038.39
YFIL_ECOLIP11289
yfiL
b2602 JW5412 Uncharacterized protein yfiL (URF2)Outer membrane Lipoprotein (experimental) Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLyesyesY260.211SpII1612.903Pos+2=Q00Yn5-14c19/20oUnknown2.559.3
YJJA_ECOLIP18390
yjjA
b4360 JW5795 Uncharacterized protein yjjA (Protein P-18) Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY260.86SpI2523.6068-00Yn8-19c25/26oUnknown2.5100.08
FLIO_ECOLIP22586
fliO

flaP flbD

flaP flbDb1947 JW5316 Flagellar protein fliOIntegral Inner Membrane BBinoutout/inIM (predicted)Cell membrane | Single-pass membrane proteinBacterial flagellum basal bodyMembrane anchoredflagellumSRPSECnoyesN270.161TMH00.0866025-110o18-39iCytoplasmicMembrane9.9714.69
YAFK_ECOLIP0AA99
yafK
b0224 JW0214 Putative L,D-transpeptidase YafK (EC 2.-.-.-)PeriplasmicGN/APeriplasmicSECyesyesY200.74SpI1910.678-10Yn4-22c27/28oUnknown2.525.56
YAGU_ECOLIP0AAA1
yagU
b0287 JW0281 Inner membrane protein yagUCell inner membrane Integral inner membrane (experimental).PMID: 16079137 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN300.316SpI293.31288-340i14-35o98-120i132-150o162-182iCytoplasmicMembrane1044.15
WZXE_ECOLIP0AAA7
wzxE

wzx yifJ

wzx yifJb3792 JW3766 Protein wzxEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.139TMH05.34268-1211Yn6-16c21/22o45-67i79-105o117-138i145-164o176-195i216-237o257-278i299-322o334-355i362-382o394-412iCytoplasmicMembrane10
YBHL_ECOLIP0AAC4
ybhL
b0786 JW0769 Inner membrane protein ybhLIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.151TMH05.60898-770i20-42o57-77i89-107o113-131i143-161o167-186i207-230oCytoplasmicMembrane10
YCCA_ECOLIP0AAC6
yccA
b0970 JW0953 Modulator of FtsH protease YccAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.268TMH07.78202-770i20-41o47-67i74-94o106-126i133-154o160-184i196-216oCytoplasmicMembrane10
MTR_ECOLIP0AAD2
mtr
b3161 JW3130 Tryptophan-specific transport protein (Tryptophan permease)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN470.192TMH019.2156-11110i12-34o40-65i86-107o127-145i152-170o190-207i228-246o284-309i321-340o346-367i388-410oCytoplasmicMembrane10
YCCB_ECOLIP24244
yccB
b4592 JW0961.1 b0979.1Uncharacterized protein yccBOuter membrane.Experimentally verified orientation: N(out)-C(in).PMID: 19121005 PMID: 21778229Integral Inner Membrane BBinoutout/inN/AMembrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN210.107TMH02.07613-110o6-24iCytoplasmicMembrane9.82
YIGF_ECOLIP27842
yigF
b3817 JW3790 Uncharacterized protein yigFIntegral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECnoyesN400.108CYT0-0.200913-220i52-72o78-107iCytoplasmicMembrane9.8225.78
YIGG_ECOLIP27843
yigG
b3818 JW5590 Uncharacterized protein yobICell inner membrane Integral inner membrane (experimental).PMID: 11867724 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN420.121TMH05.14332-440i18-35o41-57i69-91o103-121iCytoplasmicMembrane10
TYRP_ECOLIP0AAD4
tyrP
b1907 JW1895 Tyrosine-specific transport protein (Tyrosine permease)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN290.164TMH011.6392-1110Yn6-17c29/30o39-58i78-100o120-141i148-169o181-204i216-235o273-298i310-330o336-355i376-399oCytoplasmicMembrane10
SDAC_ECOLIP0AAD6
sdaC

dcrA

dcrAb2796 JW2767 Serine transporterIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN360.132TMH010.9503-11110i24-43o49-67i100-120o140-157i164-187o207-229i250-273o300-324i345-364o370-394i406-428oCytoplasmicMembrane10
TDCC_ECOLIP0AAD8
tdcC
b3116 JW3087 Threonine/serine transporter tdcC (H(+)/threonine-serine symporter)Integral inner membrane (experimental). Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 15919996 PMID: 25403562 PMID: 25403562Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECYfgMyesyesN350.131TMH09.58087-11110i23-42o48-66i97-116o136-155i167-186o206-234i255-278o317-337i358-379o391-409i421-440oCytoplasmicMembrane10
CYCA_ECOLIP0AAE0
cycA

dagA ytfD

dagA ytfDb4208 JW4166 D-serine/D-alanine/glycine transporterIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN530.108TMH03.35425-12120i28-45o51-71i108-130o136-154i166-187o207-231i252-274o286-311i347-365o371-397i417-435o441-460iCytoplasmicMembrane10
YAAI_ECOLIP28696
yaaI
b0013 JW0012 UPF0412 protein yaaIPeriplasmicGN/APeriplasmicSECyesyesY240.727SpI239.88216-00Yn4-15c23/24oUnknown2.559.32
YHHH_ECOLIP28911
yhhH
b3483 JW3449 Uncharacterized protein yhhH (ORF-B2)Integral Inner Membrane BBoutin/outIM (predicted)Membrane anchoredSRPSECnoyesN320.366CYT0-0.200913-110i12-30oUnknown252
YBFC_ECOLIP28915
ybfC
b0704 JW0693 Uncharacterized protein ybfCPrediction of three weak transmembrane helixes (one with 0.6 probability) in Phobius.PeriplasmicGN/APeriplasmicSECnoyesY200.787SpI193.48234-00Yn4-15c19/20oUnknown248.41
YDCC_ECOLIP28917
ydcC
b1460 JW1455 H repeat-associated protein ydcC (ORF-H)PeriplasmicGIM (predicted)CytoplasmicSECTransposase, Rhs ElementnonoN420.169CYT0-0.200913-00Yn29-38c43/44oCytoplasmic8.9623.57
PROY_ECOLIP0AAE2
proY

yajM

yajMb0402 JW5055 Proline-specific permease proYIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN480.136TMH06.32309-12120i16-34o40-59i95-118o124-142i154-176o196-218i239-258o278-298i330-350o356-377i398-421o427-445iCytoplasmicMembrane10
ARCD_ECOLIP0AAE5
ydgI
b1605 JW1597 Putative arginine/ornithine antiporterNucleoid (experimental). Integral inner membrane (experimental).PMID: 23138451 PMID: 15919996Nucleoid, Integral Inner Membrane N, Bout/outIM (predicted)Cell inner membrane | Multi-pass membrane protein.Integral Membrane Protein (Experimental)SRPSECyesyesN220.206TMH08.33129-1312Yn5-16c25/26o41-61i82-107o127-145i157-178o204-222i234-257o283-311i332-352o358-378i385-402o408-426i441-459oCytoplasmicMembrane10
CADB_ECOLIP0AAE8
cadB
b4132 JW4093 Probable cadaverine/lysine antiporterIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN180.152TMH09.00829-12120i7-28o40-58i94-115o121-139i146-169o189-208i220-238o258-283i323-344o350-371i383-401o407-424iCytoplasmicMembrane10
POTE_ECOLIP0AAF1
potE
b0692 JW0679 Putrescine-ornithine antiporter (Putrescine transport protein)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN500.144TMH05.94665-12120i12-30o42-60i90-110o122-140i152-172o184-204i225-249o276-300i321-345o351-374i386-404o410-428iCytoplasmicMembrane10
MARB_ECOLIP31121
marB
b1532 JW1525 Multiple antibiotic resistance protein marBPeriplasmicGN/APeriplasmicSECnoyesY220.602SpI2118.3001-00Yn4-15c21/22oUnknown2.560.83
YDEI_ECOLIP31130
ydeI
b1536 JW1529 Uncharacterized protein ydeIPeriplasmicGN/APeriplasmicSECnoyesY200.882SpI1913.7979-00Yn3-14c19/20oUnknown2.5100.85
YIDI_ECOLIP31446
yidI
b3677 JW3653 Inner membrane protein yidIIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN360.149TMH00.964289-330i12-30o78-98i110-143oCytoplasmicMembrane1027.57
CBRB_ECOLIP31468
cbrB

yieI

yieIb3716 JW3694 Inner membrane protein CbrB (CreB-regulated gene B protein)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN340.114TMH014.4652-440i12-32o38-69i81-100o120-138iCytoplasmicMembrane1025.64
MDLB_ECOLIP0AAG5
mdlB

mdl

mdlb0449 JW5061 Multidrug resistance-like ATP-binding protein mdlB (EC 3.6.3.44)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN380.136SpI374.21577-44Yn25-32c37/38o61-81i138-161o167-185i250-273oCytoplasmicMembrane106.66
FTSH_ECOLIP0AAI3
ftsH

hflB mrsC std tolZ

hflB mrsC std tolZb3178 JW3145 ATP-dependent zinc metalloprotease FtsH (EC 3.4.24.-) (Cell division protease FtsH)Cell inner membrane Cell inner membrane Inner membrane (experimental). Integral inner membrane (experimental). Integral Membrane Protein Integral Membrane Protein Integral Membrane Protein Integral Membrane ProteinTrigger factor-sensitive aggregation. PMID: 8444797 PMID: 1925026 PMID: 8106505 PMID: 8106505 PMID: 7781608PMID: 7500942 PMID: 19737520 PMID: 8444797 PMID: 8947034 PMID: 15919657 PMID: 15919996 PMID: 1925026 PMID: 8106505 PMID: 8106505 PMID: 7781608Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRP,TFSECnoyesN240.152SpI212.2847-21Yn5-16c21/22o98-119iCytoplasmicMembrane1041.14
YNFG_ECOLIP0AAJ1
ynfG
b1589 JW1581 Probable anaerobic dimethyl sulfoxide reductase chain ynfG (DMSO reductase iron-sulfur subunit ynfG)Peripheral inner membrane protein facing the periplasm.The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. ynfFGH are expressed anaerobically. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. YnfH can function with DmsAB, replacing DmsC as the anchor subunit. The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.PMID: 14522592PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/APeriplasmicTAT piggybackyesyesN440.106CYT0-0.200913-000iUnknown5.4138.9
YDCD_ECOLIP31991
ydcD
b1457 JW1452 Uncharacterized protein ydcD (ORF-E2)Integral Inner Membrane BBoutin/outIM (predicted)Membrane anchoredSRPSECnoyesN320.142CYT0-0.200913-110i7-25oUnknown29.14
YIBG_ECOLIP32106
yibG
b3596 JW3570 Uncharacterized protein yibG (ORF-A3)PeriplasmicGN/APeriplasmicSECnoyesY200.567SpI190.904394-00Yn3-14c19/20oUnknown218.53
YIBI_ECOLIP32108
yibI
b3598 JW3572 Uncharacterized protein yibIIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN270.12TMH012.0249-220o6-25i46-66oCytoplasmicMembrane1033.12
YIIQ_ECOLIP32160
yiiQ
b3920 JW3891 Uncharacterized protein yiiQPeriplasmicGN/APeriplasmicSECyesyesY240.736SpI2314.7775-00Yn4-15c23/24oUnknown2.5
FDNH_ECOLIP0AAJ3
fdnH
b1475 JW1471 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit (Anaerobic formate dehydrogenase iron-sulfur subunit) (Formate dehydrogenase-N subunit beta) (FDH-N subunit beta)Membrane anchored Alpha-helcal transmembrane protein (experimental). Transmembrane helices: 1.Part of the formate dehydrogenase N (FDH-N) of Escherichia coli which is a membrane-bound enzyme comprising FdnG, FdnH, and FdnI subunits. PMID: 11884747 PMID: 16858726PMID: 15342602 PMID: 16858726 PMID: 11266632 PMID: 11884747Integral Inner Membrane BBinin/outIM (predicted)Cell membrane | Single-pass membrane proteinMembrane anchored (Experimental)SRPSECyesyesN240.116CYT0-0.200913-110i260-279oCytoplasmicMembrane1080.89
HYBA_ECOLIP0AAJ8
hybA
b2996 JW2964 Hydrogenase-2 operon protein hybAOuter membrane protein (experimental). Inner membrane. PeriplasmicTAT secretion system. Part of membrane-associated hydrogenase 4 complex. PMID: 9738917PMID: 15911532 PMID: 22313414 PMID: 21890895 PMID: 9738917Peripheral inner membrane protein facing the periplasmF2IM (predicted)PeriplasmPeriplasmic (Experimental)TAT,SECyesyesY270.442SpI2619.7463-01Yn9-20c26/27o293-311iCytoplasmicMembrane9.9739.36
NRFC_ECOLIP0AAK7
nrfC

yjcJ

yjcJb4072 JW4033 Protein nrfCPeriplasmicTAT secretion system. PMID: 15280383PMID: 17218314 PMID: 15280383PeriplasmicGN/APeriplasmic (Experimental)TATyesyesN280.447SpI277.45987-00Yn8-19c27/28oCytoplasmic1023.27
NAPG_ECOLIP0AAL3
napG

yojA yojB

yojA yojBb2205 JW2193 Ferredoxin-type protein napGPeriplasmicTAT secretion system. PMID: 11967083PMID: 17218314 PMID: 11967083PeriplasmicGN/APeriplasmic (Experimental)TATyesyesN420.316SpI410.19393-00Yn21-32c41/42oCytoplasmic8.9620.35
CYBH_ECOLIP0AAM1
hyaC
b0974 JW0956 Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunitIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.112TMH010.0819-460o20-40i61-80o100-116i128-151o157-174i186-203oCytoplasmicMembrane10
YIIX_ECOLIP32167
yiiX
b3937 JW3908 Uncharacterized protein yiiXPeriplasmicGN/APeriplasmicSECnoyesY190.818SpI1814.7441-00Yn5-13c18/19oUnknown2.527.16
YIJF_ECOLIP32668
yijF
b3944 JW3916 Uncharacterized protein yijFPeriplasmicGN/APeriplasmicSECyesyesY190.257SpI2011.5911-00Yn3-14c18/19oUnknown2.514.94
YJAH_ECOLIP32681
yjaH
b4001 JW3965 Uncharacterized protein yjaHOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLyesyesN360.151SpII306.9142Pos+2=S00Yn20-28c46/47oUnknown2.525.12
ADRA_ECOLIP0AAP1
adrA

yaiC

yaiCb0385 JW0376 Probable diguanylate cyclase AdrA (DGC) (EC 2.7.7.65)Integral Membrane Protein Integral Membrane Protein Integral Membrane ProteinPMID: 10760159 PMID: 12673057 PMID: 16585767Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein(experimental)SRPSECyesyesN200.116CYT0-0.200913-550i43-61o67-85i106-130o142-167i174-193oCytoplasmicMembrane1022.58
YBBJ_ECOLIP0AAS3
ybbJ
b0488 JW5065 Inner membrane protein ybbJIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.17TMH018.8226-330o6-23i30-49o55-72iCytoplasmicMembrane1012.04
YBDG_ECOLIP0AAT4
mscM

ybdG

ybdGb0577 JW0566 Uncharacterized protein ybdGMembrane protein. Integral inner membrane (experimental).Mechanosensitive channel of miniconductance activity (MscM).PMID: 20616037 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN390.102CYT0-0.200913-550o23-48i68-87o107-127i139-160o166-183iCytoplasmicMembrane1011.27
YBIS_ECOLIP0AAX8
ybiS
b0819 JW0803 Probable L,D-transpeptidase YbiS (EC 2.-.-.-)Peripheral outer membrane protein facing the periplasm (experimental). Periplasmic Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. YbiS has been shown to crosslink Braun's lipoprotein (Lpp) to the peptidoglycan. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 18498364 PMID: 23832002 PMID: 9298646 PMID: 22030350Peripheral outer membrane protein facing the periplasmF3N/APeriplasmPeriplasmic (Experimental)SECSURAyesyesY250.584SpI2419.8413-10Yn8-19c24/25oUnknown2.540.5
YJBG_ECOLIP32688
yjbG
b4028 JW3988 Uncharacterized protein yjbGPeriplasmicGN/APeriplasmicSECyesyesY210.827SpI2017.0485-00Yn5-15c20/21oCytoplasmicMembrane9.86
PSPG_ECOLIP32696
pspG

yjbO

yjbOb4050 JW5716 Phage shock protein GCell inner membranePMID: 19555453Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECProphage TransposasenonoN320.133TMH016.1628-220i7-37o43-64iCytoplasmicMembrane9.82
YJCB_ECOLIP32700
yjcB
b4060 JW5718 Uncharacterized protein yjcBPMID: 19555453Integral Inner Membrane BBpolyout/outIM (predicted)Integral Membrane ProteinSRPSECyesyesN650.12TMH09.92398-220o12-29i36-56oCytoplasmicMembrane9.8245.88
NRFF_ECOLIP32711
nrfF

yjcM

yjcMb4075 JW4036 Formate-dependent nitrite reductase complex subunit nrfFIntegral Inner Membrane BBoutout/inIM (predicted)PeriplasmMembrane anchoredSRPSECnoyesY190.845SpI1816.61-11Yn4-14c18/19o103-121iCytoplasmicMembrane1020.08
ELYC_ECOLIP0AB01
ycbC
b0920 JW0903 Uncharacterized protein ycbCShown to play a critical role in the metabolism of the essential lipid carrier used for the biogenesis of cell wall and other bacterial surface polysaccharides.PMCID: PMC3879167Integral Inner Membrane BBpolyout/outIM (predicted)Integral Membrane ProteinSRPSECyesyesN600.155TMH09.49418-220o12-32i41-59oCytoplasmicMembrane9.8224.71
YMBA_ECOLIP0AB10
ymbA
b0952 JW5127 Uncharacterized lipoprotein ymbAputative protase (enterobacterium homolog)Inner Membrane LipoproteinEN/ACell membrane | Lipid-anchorCytoplasmicSECLOLyesyesN200.205SpII1522.8198Pos+2=S00Yn4-14c19/20oUnknown282.33
EFEO_ECOLIP0AB24
efeO

ycdO

ycdOb1018 JW1003 UPF0409 protein ycdOPeriplasm Periplasmic Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 16551627 PMID: 19953543 PMID: 12107143 PMID: 22030350 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY270.767SpI2618.2235-00Yn9-18c26/27oPeriplasmic1042.79
YCEB_ECOLIP0AB26
yceB
b1063 JW1050 Uncharacterized lipoprotein yceBLipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN550.134SpII1816.4921Pos+2=N00Yn4-15c19/20oUnknown245.35
LPOB_ECOLIP0AB38
lpoB

ycfM

ycfMb1105 JW5157 Uncharacterized protein ycfMLipoprotein (experimental). Cell outer membrane Cell outer membrane Outer membrane protein (experimental).The two OM Lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 21183073 PMID: 21183074 PMID: 20932056Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLyesyesY240.258SpII1923.7759Pos+2=V00Yn8-18c22/23oUnknown296.82
NRFG_ECOLIP32712
nrfG

yjcN

yjcNb4076 JW4037 Formate-dependent nitrite reductase complex subunit nrfGOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLyesyesN350.209SpII216.47674Pos+2=V10Yn11-22c30/31oUnknown264.76
MOKC_ECOLIP33236
mokC

gefL

gefLb0018 JW5879 Regulatory protein mokCIntegral Inner Membrane BBoutin/outN/AMembrane | Single-pass membrane proteinPeriplasmicSRPSECnoyesN410.287SpI401.37208-110i25-44oPeriplasmic9.97
YEHE_ECOLIP33344
yehE
b2112 JW2099 Uncharacterized protein yegJYeh operon is homolog to fim operon (yehd is fimI homolog).PMID: 20345943PeriplasmicGN/APeriplasmicSECnoyesY230.529SpI2211.8793-00Yn6-17c22/23oCytoplasmicMembrane9.8649.18
PPDA_ECOLIP33554
ppdA
b2826 JW2794 Prepilin peptidase-dependent protein APeriplasmicGN/APeriplasmicSECyesyesN270.172TMH07.10154-110i12-33oCytoplasmicMembrane9.8210.54
LAPB_ECOLIP0AB58
yciM
b1280 JW1272 Uncharacterized protein yciMInner membrane. Potential TAT secreted protein. Required to maintain cell envelope integrity. Heat shock protein involved in the assembly of LPS. In pull-down experiments, LapA and LapB co-purified with LPS, Lpt proteins, FtsH (protease), DnaK, and DnaJ (chaperones). PMID: 25403562PMID: 24187084 PMID: 25403562Integral Inner Membrane B-Soluble IM FractionPeriplasmicSECyesyesN250.264CYT0-0.200913-00Yn4-11c16/17oCytoplasmic8.969.37
PNTB_ECOLIP0AB67
pntB
b1602 JW1594 NAD(P) transhydrogenase subunit beta (EC 1.6.1.2) (Nicotinamide nucleotide transhydrogenase subunit beta) (Pyridine nucleotide transhydrogenase subunit beta)Integral inner membrane (experimental). Integral inner membrane (experimental).PMID: 14660605 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.125TMH09.6368-990o6-24i36-51o57-74i86-105o125-143i163-180o186-207i214-234o240-260iCytoplasmicMembrane10
APBE_ECOLIP0AB85
apbE

yojK yojL

yojK yojLb2214 JW5368 Thiamine biosynthesis lipoprotein ApbELipoprotein (experimental).Proven signal peptidase II substrate.PMID: 15174130Inner Membrane LipoproteinEIM (predicted)Cell membrane | Lipid-anchorInner membrane lipoproteinSECLOLyesyesN280.154SpII1912.0176Pos+2=D00Yn8-18c22/23oUnknown265.6
ARSB_ECOLIP0AB93
arsB

arsF

arsFb3502 JW3469 Arsenical pump membrane protein (Arsenic efflux pump protein)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN440.23TMH08.42262-1110Yn2-17c35/36o51-70i91-109o115-132i139-163o175-198i222-240o246-263i275-296o316-334i401-423oCytoplasmicMembrane10
YBBC_ECOLIP33668
ybbC
b0498 JW0487 Uncharacterized protein ybbCOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesY180.367SpII178.97285Pos+2=N00Yn6-17c25/26oUnknown2.548.94
YBBD_ECOLIP33669
ybbD
b0501 JW0489 Putative uncharacterized protein ybbDIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECpseudogene (Uniprot) nonoN350.114TMH09.05171-110o12-32iCytoplasmicMembrane9.8246.29
ASR_ECOLIP36560
asr
b1597 JW5826 Acid shock proteinPeriplasmicPMID: 12670971PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY220.907SpI2128.9272-00Yn4-15c21/22oPeriplasmic10
PPDD_ECOLIP36647
ppdD
b0108 JW0104 Prepilin peptidase-dependent protein DIntegral Inner Membrane BBoutin/outIM (predicted)FimbriumMembrane anchoredSRPSECnoyesN530.143TMH04.07217-110i12-30oExtracellular9.719.63
ATP6_ECOLIP0AB98
atpB

papD uncB

papD uncBb3738 JW3716 ATP synthase subunit a (ATP synthase F0 sector subunit a) (F-ATPase subunit 6)Integral inner membrane (experimental). Integral Membrane ProteinExhibites aberrant localization by microscopy in the absence of YidC. PMID: 10806384PMID: 22108384 PMID: 15263011 PMID: 15919996 PMID: 10806384Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPYidCyesyesN670.123CYT0-0.200913-550o36-60i100-118o147-166i211-233o239-264iCytoplasmicMembrane107.26
ATPF_ECOLIP0ABA0
atpF

papF uncF

papF uncFb3736 JW3714 ATP synthase subunit b (ATP synthase F(0) sector subunit b) (ATPase subunit I) (F-type ATPase subunit b) (F-ATPase subunit b) Membrane anchoredPMID: 15263011 PMID: 21541338 PMID: 6278247Integral Inner Membrane BBinout/inSoluble IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchored (Experimental)SRPSECnoyesN320.35CYT0-0.200913-110o6-26iCytoplasmicMembrane1019.55
ATMA_ECOLIP0ABB8
mgtA

corB mgt

corB mgtb4242 JW4201 Magnesium-transporting ATPase, P-type 1 (EC 3.6.3.2) (Mg(2+) transport ATPase, P-type 1)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN470.122CYT0-0.200913-780i89-112o118-137i291-309o315-342i768-791o803-821i833-857o869-891iCytoplasmicMembrane1050.58
HFLC_ECOLIP0ABC3
hflC

hflA

hflAb4175 JW4133 Uncharacterized protein yqeLCell inner membrane Membrane anchored N-terminally located transmembrane segment. PMID: 9792691 Translocation of the C-terminal domains of HflKC was retarded markedly when sodium azide was added to inhibit SecA ATPase and blocked almost completely in secY- or secD-defective mutant cells. PMID: 9792691PMID: 8947034 PMID: 16834335 PMID: 9792691 PMID: 9792691Integral Inner Membrane BBinout/inSoluble IM FractionCell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SRPSECProphage Transposase (by homology to BL21)yesyesN250.135CYT0-0.200913-110o6-23iCytoplasmic8.9646.95
FXSA_ECOLIP37147
fxsA

yjeG

yjeGb4140 JW4100 UPF0716 protein fxsA (Suppressor of F exclusion of phage T7)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN640.114TMH05.51241-330o6-24i29-47o78-101iCytoplasmicMembrane1025.95
HOKA_ECOLIP37305
hokA

yiaZ

yiaZb4455 JW3526 Protein hokAPotentially toxic protein.PMID: 19121005Integral Inner Membrane BBoutin/outIM (predicted)Membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN250.11CYT0-0.200913-110i7-24oPeriplasmic9.24110.3
HFLK_ECOLIP0ABC7
hflK

hflA

hflAb4174 JW4132 Modulator of FtsH protease HflKCell inner membrane Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 N-terminally located transmembrane segment. PMID: 9792691 Translocation of the C-terminal domains of HflKC was retarded markedly when sodium azide was added to inhibit SecA ATPase and blocked almost completely in secY- or secD-defective mutant cells. PMID: 9792691PMID: 8947034 PMID: 21541338 PMID: 9792691 PMID: 9792691Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchoredSRPSECProphage TransposaseyesnoN30.101CYT0-0.200913-110i80-100oUnknown5.4820.03
BETT_ECOLIP0ABC9
betT
b0314 JW0306 High-affinity choline transport proteinCell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN370.108TMH011.6116-12120i16-35o55-79i91-115o143-166i194-215o235-253i265-290o319-338i350-368o403-422i448-467o479-497iCytoplasmicMembrane10
YEAV_ECOLIP0ABD1
yeaV
b1801 JW5293 Uncharacterized protein ynbGIntegral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN190.119TMH02.33182-1011Yn2-13c18/19o34-59i80-104o136-158i170-191o203-221i228-246o258-277i289-308o348-371i392-412o418-438iCytoplasmicMembrane10
C561_ECOLIP0ABE5
cybB
b1418 JW5224 Cytochrome b561 (Cytochrome b-561)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN230.142TMH07.83021-440i12-33o39-62i82-106o143-162iCytoplasmicMembrane1031.95
YHHL_ECOLIP37614
yhhL
b3466 JW5683 Uncharacterized protein yhhLIntegral Inner Membrane BBinoutout/outIM (predicted)Integral Membrane ProteinSRPSECnoyesN250.114TMH01.98334-21Yn8-19c24/25o34-51iCytoplasmicMembrane9.8247.43
YHHM_ECOLIP37615
yhhM
b3467 JW3432 Uncharacterized protein yhhMSingle transmembrane helice predicted which would be removed upon signal peptide cleavage IM-cter inside.Integral Inner Membrane BBinout/inN/AMembrane anchoredSRPSECyesyesN320.135CYT0-0.200913-110o6-24iUnknown275.11
YHHQ_ECOLIP37619
yhhQ
b3471 JW3436 Inner membrane protein yhhQIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.133TMH011.3075-660i12-31o43-64i71-92o104-127i139-165o185-206iCytoplasmicMembrane10
YIAM_ECOLIP37674
yiaM
b3577 JW3549 2,3-diketo-L-gulonate TRAP transporter small permease protein yiaMIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN230.132TMH06.97669-440i7-28o40-59i80-101o113-137iCytoplasmicMembrane1013.66
PGSA_ECOLIP0ABF8
pgsA
b1912 JW1897 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5) (Phosphatidylglycerophosphate synthase) (PGP synthase)Integral inner membrane (experimental). Integral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 12558186 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN350.17TMH012.5336-460i7-27o33-52i64-83o89-108i120-142o148-169iCytoplasmicMembrane107.29
CDSA_ECOLIP0ABG1
cdsA

cds

cdsb0175 JW5810 Phosphatidate cytidylyltransferase (EC 2.7.7.41) (CDP-DAG synthase) (CDP-DG synthase) (CDP-diacylglycerol synthase) (CDS) (CDP-diglyceride pyrophosphorylase) (CDP-diglyceride synthase) (CTP:phosphatidate cytidylyltransferase)Integral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. PMID: 15919996PMID: 9008159 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRP (exp)SECyesyesN300.287TMH018.3357-88Yn6-13c18/19o28-44i56-73o93-113i120-141o153-171i192-210o216-234i264-282oCytoplasmicMembrane10
FTSW_ECOLIP0ABG4
ftsW
b0089 JW0087 Cell division protein ftsWInner membrane. Cell inner membrane Cell inner membrane Cell inner membrane Integral inner membrane (experimental).Interacts directly with FtsN. The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex.PMID: 20847002 PMID: 20497333 PMID: 9603865 PMID: 12423747 PMID: 11807049 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN670.137TMH010.7628-109Yn15-26c32/33o48-66i87-104o110-129i175-192o198-215i222-239o307-328i340-364o376-395iCytoplasmicMembrane10
RODA_ECOLIP0ABG7
mrdB

rodA

rodAb0634 JW0629 Rod shape-determining protein rodAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.198TMH06.46483-990o20-38i50-68o74-95i136-154o160-176i183-202o263-289i310-332o338-359iCytoplasmicMembrane10
CORA_ECOLIP0ABI4
corA
b3816 JW3789 Magnesium transport protein CorAIntegral inner membrane (experimental). Exhibites aberrant localization by microscopy in the absence of YidC. Single particle electron microscopy analysis and two-dimensional crystals of PPD (large soluble periplasmic domain) of CorA indicated that E. coli CorA PPD is a pyramid-like homotetramer with a central cavity.PMID: 22108384 PMID: 15919996 PMID: 16835234Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN270.101CYT0-0.200913-220i255-278o290-310iCytoplasmicMembrane1065.69
YIAT_ECOLIP37681
yiaT
b3584 JW3556 Putative outer membrane protein yiaTPMID: 20639525Outer Membrane b-barrel proteinHN/ACell outer membrane | Peripheral membrane proteinOuter Membrane β-barrel proteinSECnoyesY220.602SpI2111.315-00Yn7-17c21/22oOuterMembrane1022.26
SIEB_ECOLIP38392
sieB
b1353 JW5209 Superinfection exclusion protein BCell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Membrane | Peripheral membrane proteinIntegral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN420.105TMH03.43476-220i20-38o50-70iCytoplasmicMembrane1013.63
CYOB_ECOLIP0ABI8
cyoB
b0431 JW0421 Ubiquinol oxidase subunit 1 (EC 1.10.3.-) (Cytochrome o subunit 1) (Cytochrome o ubiquinol oxidase subunit 1) (Oxidase BO(3) subunit 1) (Ubiquinol oxidase chain A) (Ubiquinol oxidase polypeptide I)Integral inner membrane (experimental). Cell inner membrane Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 11017202 PMID: 11266632 PMID: 22108384 PMID: 6365921 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN310.108TMH010.2812-14140o16-38i59-79o103-126i146-170o190-213i233-255o275-298i310-334o346-368i380-403o415-436i457-479o491-518i593-626oCytoplasmicMembrane10
CYOA_ECOLIP0ABJ1
cyoA
b0432 JW0422 Ubiquinol oxidase subunit 2 (EC 1.10.3.-) (Cytochrome o subunit 2) (Cytochrome o ubiquinol oxidase subunit 2) (Ubiquinol oxidase chain B) (Oxidase BO(3) subunit 2) (Ubiquinol oxidase polypeptide II)Lipoprotein (experimental). Alpha-helcal transmembrane protein (experimental). Cell inner membrane Cell inner membrane Integral inner membrane (experimental). Integral inner membrane (experimental). Proven signal peptidase II substrate PMID: 15174130. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 The SRP interacts with the first TM of CyoA (rather than with its signal sequence) to mediate targeting of CyoA to the Sec/YidC insertion site in the inner membrane. PMID: 16481320 Efficient insertion of in vitro synthesized pre-CyoA into proteoliposomes requires YidC, SecYEG, and SecA and occurs independently of the proton motive force. PMID: 16513637PMID: 15174130 PMID: 11266632 PMID: 11017202 PMID: 6365921 PMID: 16079137 PMID: 15919657 PMID: 15919996 PMID: 21541338 PMID: 16481320 PMID: 16513637Integral Inner Membrane, Inner Membrane LipoproteinB, EBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN310.189TMH011.3494-32Yn10-22c27/28o43-67i88-108oCytoplasmicMembrane1019.49
CYOC_ECOLIP0ABJ3
cyoC
b0430 JW0420 Cytochrome o ubiquinol oxidase subunit 3 (EC 1.10.3.-) (Cytochrome o ubiquinol oxidase subunit III) (Ubiquinol oxidase chain C)Integral inner membrane (experimental). Integral inner membrane (experimental).PMID: 11017202 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN140.175TMH03.44396-550i29-50o70-91i98-118o138-165i177-201oCytoplasmicMembrane10
YTFJ_ECOLIP39187
ytfJ
b4216 JW4175 Uncharacterized protein ytfJPeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic(experimental)SECyesyesY210.734SpI2013.6826-00Yn6-14c22/23oUnknown2.518.26
YJEO_ECOLIP39284
yjeO
b4158 JW4119 Toxin yoeB (EC 3.1.-.-)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN260.227TMH07.24951-32Yn6-17c25/26o55-72i79-98oCytoplasmicMembrane1056.51
YTFB_ECOLIP39310
ytfB
b4206 JW5745 Uncharacterized protein ytfBIntegral Inner Membrane BBinoutin/outIM (predicted)Membrane | Single-pass membrane proteinCytoplasmicSRPSECnoyesN570.108CYT0-0.200913-010i30-48oExtracellular9.7157.94
CYDA_ECOLIP0ABJ9
cydA

cyd-1

cyd-1b0733 JW0722 Cytochrome d ubiquinol oxidase subunit 1 (EC 1.10.3.-) (Cytochrome bd-I oxidase subunit I) (Cytochrome d ubiquinol oxidase subunit I)Cell inner membrane Cell inner membrane Inner membrane (experimental). Integral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15013751 PMID: 16079137 PMID: 15919657 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN370.106TMH09.54728-990o20-42i54-71o91-116i128-151o184-208i220-238o388-409i421-444o471-494iCytoplasmicMembrane10
CYDB_ECOLIP0ABK2
cydB

cyd-2

cyd-2b0734 JW0723 Cytochrome d ubiquinol oxidase subunit 2 (EC 1.10.3.-) (Cytochrome bd-I oxidase subunit II) (Cytochrome d ubiquinol oxidase subunit II)Cell inner membrane Cell inner membrane Integral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15013751 PMID: 16079137 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN290.118TMH010.5085-880i9-28o77-99i119-141o161-181i202-226o264-283i290-315o335-360iCytoplasmicMembrane10
CSGB_ECOLIP0ABK7
csgB
b1041 JW1024 Minor curlin subunitCell surface appendage Cell surface appendage Cell surface appendageCurli component. CsgB localization requires at least one component of the curli secretion apparatus, CsgF. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041PMID: 16704339 PMCID: PMC2630086 PMID: 21645131 PMID: 19131513 PMID: 19131513 PMID: 9383186 PMID: 9312041Peripheral outer membrane protein facing the extra-cellular spaceF4N/AFimbrium. Part of the curli surface structure.Cell surface appendage (Experimental)CurliSECCurliyesyesY220.53SpI2116.29-00Yn5-13c21/22oExtracellular9.65
NRFA_ECOLIP0ABK9
nrfA
b4070 JW4031 Cytochrome c-552 (EC 1.7.2.2) (Ammonia-forming cytochrome c nitrite reductase) (Cytochrome c nitrite reductase)Periplasmic Disulfide bond-containing protein PMID: 25664544PMID: 793493 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.665SpI2614.744-00Yn11-21c26/27oPeriplasmic1025.45
YJHE_ECOLIP39355
yjhE
b4282 Putative uncharacterized protein yjhEIntegral Inner Membrane B-IM (predicted)Integral Membrane ProteinSRPSECpseudogene (Uniprot) nonoN380.334CYT0-0.200913-220i22-49o55-76iCytoplasmicMembrane1015.12
YJIH_ECOLIP39379
yjiH
b4330 JW5783 Uncharacterized protein yjiHIntegral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN380.143TMH05.26808-440i21-39o86-104i116-135o176-201iCytoplasmicMembrane9.8216.56
YGJQ_ECOLIP42598
ygjQ
b3086 JW3057 Uncharacterized protein ygjQIntegral Inner Membrane BBoutin/outN/AMembrane | Single-pass membrane proteinPeriplasmicSRPSECnoyesN370.199SpI366.02153-10Yn21-31c36/37oCytoplasmicMembrane9.82
NRFB_ECOLIP0ABL1
nrfB

yjcI

yjcIb4071 JW4032 Cytochrome c-type protein nrfBPeriplasmic Disulfide bond-containing protein PMID: 25664544PMID: 15280383 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY330.498SpI256.62799-00Yn7-18c23/24oPeriplasmic9.8416.12
NAPB_ECOLIP0ABL3
napB

yejY

yejYb2203 JW5367 Diheme cytochrome c napBPeriplasm Periplasm Periplasm Periplasm Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 10548535 PMID: 17130127 PMID: 11844760 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY280.825SpI2714.6891-00Yn9-20c27/28oPeriplasmic10
NAPC_ECOLIP0ABL5
napC

yejX

yejXb2202 JW2190 Cytochrome c-type protein napCPeriplasm Inner membrane Periplasmic with N-terminal membrane anchor Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 Electrons are fed into the NapAB dimer from a membrane-anchored tetra-heme cytochrome c,encoded by the napC gene. PMID: 8830238 PMID: 12186631PMID: 11844760 PMID: 8830238 PMID: 12186631Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Single-pass membrane proteinPeriplasmic with N-terminal membrane anchor (Experimental)SRPSECyesyesN520.116TMH06.01984-110i21-44oCytoplasmicMembrane1014.18
CCMB_ECOLIP0ABL8
ccmB

yejV

yejVb2200 JW2188 Heme exporter protein B (Cytochrome c-type biogenesis protein CcmB)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN200.11TMH09.26722-660i21-39o45-70i82-103o123-145i157-180o192-215iCytoplasmicMembrane104.38
CCMC_ECOLIP0ABM1
ccmC

yejT yejU

yejT yejUb2199 JW2187 Heme exporter protein C (Cytochrome c-type biogenesis protein CcmC)Integral inner membrane (experimental). Exhibites aberrant localization by microscopy in the absence of YidC. CcmC, CcmE, and heme interact directly with each other, establishing a periplasmic heme delivery pathway for cytochrome c maturation. PMID: 11384983PMID: 22108384 PMID: 15919996 PMID: 11384983Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN290.15TMH014.9856-660i21-43o63-84i91-113o133-149i161-182o202-221iCytoplasmicMembrane10
YGJV_ECOLIP42603
ygjV
b3090 JW3061 Inner membrane protein ygjVIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN560.156TMH04.26984-550o6-24i36-59o71-89i101-124o136-157iCytoplasmicMembrane10
YQJB_ECOLIP42615
mzrA

ecfM yqjB

ecfM yqjBb3096 JW3067 Uncharacterized protein yqjBCell inner membrane Cell inner membranePMID: 19432797 PMID: 20889743Integral Inner Membrane BBoutin/outN/AMembrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN300.154SpI294.521-10Yn11-22c27/28oUnknown2.514.58
YQJC_ECOLIP42616
yqjC
b3097 JW5516 Protein yqjCPeriplasmicGN/APeriplasmicSECyesyesY210.508SpI209.52426-00Yn5-15c20/21oUnknown2.579.95
YQJF_ECOLIP42619
yqjF
b3101 JW5850 Inner membrane protein yqjFIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.118TMH08.80873-440i7-26o46-66i73-91o103-122iCytoplasmicMembrane10
CCMH_ECOLIP0ABM9
ccmH

yejP

yejPb2194 JW2182 Cytochrome c-type biogenesis protein CcmHIntegral inner membrane (experimental). Integral inner memrbane. Might be is co-translationally translocated through Sec translocon. CcmH is a periplasm-resident oxidoreductase anchored to the inner membrane by two transmembrane segments. Both N- and C-terminal domains are located and function in the periplasmic compartment. PMID: 22789558 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 15838024 PMID: 15919996 PMID: 22789558 PMID: 15838024Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesY190.795SpI188.87226-32Yn3-13c18/19o102-120i147-166oCytoplasmicMembrane1041.31
KDGL_ECOLIP0ABN1
dgkA
b4042 JW4002 Diacylglycerol kinase (DAGK) (EC 2.7.1.107) (Diglyceride kinase) (DGK)Integral inner membrane (experimental). Integral Membrane Protein Integral Membrane ProteinPMID: 15919996 PMID: 2984194 PMID: 8071224Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN540.112CYT0-0.200913-230i30-51o57-80i100-121oCytoplasmicMembrane1031.25
DCUA_ECOLIP0ABN5
dcuA

genA

genAb4138 JW5735 Anaerobic C4-dicarboxylate transporter dcuAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN280.186TMH07.74612-11110o6-38i45-66o86-109i130-152o164-190i225-243o255-277i289-306o326-345i357-381o410-431iCytoplasmicMembrane10
DCUB_ECOLIP0ABN9
dcuB

genF

genFb4123 JW4084 Anaerobic C4-dicarboxylate transporter dcuBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN680.125TMH08.74519-1110Yn2-13c17/18o27-44i51-71o91-118i139-162o174-195i236-254o266-288i300-318o342-372i421-445oCytoplasmicMembrane10
PTPC2_ECOLIP42905
agaW
b3134 Putative N-acetylgalactosamine permease IIC component 2 (EIIC-Aga') (PTS system N-acetylgalactosamine-specific EIIC component 2)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006)noyesN700.132TMH06.59862-330o6-26i33-56o94-116iCytoplasmicMembrane109.53
YGAH_ECOLIP43667
ygaH
b2683 JW2658 Uncharacterized protein ygaHIntegral Inner Membrane BBpolyout/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN180.142CYT0-0.200913-33Yn5-15c20/21o36-57i69-85o91-109iCytoplasmicMembrane10
YHCE_ECOLIP45421
yhcE
b4569 b3217Putative uncharacterized protein yhcEPotential fimbrial yhc operon (class β); Uknown function.PMID: 20345943PeriplasmicGN/ACell outer membrane | Multi-pass membrane proteinPeriplasmicfimbriaeSECpseudogene (Uniprot)nonoY200.69SpI194.02903-00Yn4-11c16/17oUnknown220.63
YHCF_ECOLIP45422
yhcF
b3219 JW3188 Uncharacterized protein yehDPotential fimbrial yhc operon (class β); Fimbrial Subunit.PMID: 20345943PeriplasmicGN/APeriplasmicfimbriaeSECnoyesY210.835SpI2016.471-00Yn6-16c20/21oUnknown2.57.33
DCUC_ECOLIP0ABP3
dcuC
b0621 JW0613/JW0616 Anaerobic C4-dicarboxylate transporter dcuCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN560.117TMH00.537839-12120i5-22o28-46i79-98o118-144i156-175o195-218i238-260o266-288i309-333o339-363i406-424o436-458iCytoplasmicMembrane103.79
DEDA_ECOLIP0ABP6
dedA
b2317 JW2314 Protein dedA (Protein DSG-1)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC. Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF.PMID: 22108384 PMID: 15919996 PMID: 23086209Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN200.121CYT0-0.200913-440i27-49o69-92i156-178o190-208iCytoplasmicMembrane10
YIJE_ECOLIP0ABT8
yijE
b3943 JW5557 Uncharacterized inner membrane transporter yiJEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN300.12TMH07.7108-10100i12-29o35-59i71-91o97-118i125-141o153-171i183-205o217-239i246-266o272-289iCytoplasmicMembrane10
EXBB_ECOLIP0ABU7
exbB
b3006 JW2974 Biopolymer transport protein exbBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.153CYT0-0.200913-330o21-43i132-157o177-198iCytoplasmicMembrane1014.97
TOLQ_ECOLIP0ABU9
tolQ

fii

fiib0737 JW0727 Protein tolQIntegral inner membrane (experimental). Integral Membrane Protein Integral Membrane ProteinSignal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. PMID: 8300535 PMID: 8331075PMID: 9008159 PMID: 15919996 PMID: 8300535 PMID: 8331075Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRP (exp)SECyesyesN340.244TMH02.05597-330o15-35i139-159o171-192iCytoplasmicMembrane109.25
YHDT_ECOLIP45566
yhdT
b3257 JW3225 Uncharacterized protein yhdTIntegral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN300.15TMH015.9222-220i12-33o45-65iCytoplasmicMembrane9.8243.21
YBCI_ECOLIP45570
ybcI
b0527 JW0516 Inner membrane protein ybcIIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.121TMH04.35035-440i27-47o59-78i85-103o149-170iCytoplasmicMembrane104.77
HOFP_ECOLIP45750
hofP

yrfA

yrfAb3392 JW5694 Uncharacterized protein yrfANo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.PeriplasmicGIM (predicted-detected)CytoplasmicSECnoyesN210.138CYT0-0.200913-00Yn6-16c20/21oUnknown2
YRFB_ECOLIP45751
hofO

yrfB

yrfBb3393 JW3356 Uncharacterized protein yrfBIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECyesyesN400.213TMH06.9432-110o20-38iUnknown219.63
SFMA_ECOLIP0ABW5
sfmA
b0530 JW0519 Sfm fimbrial protein, A chain (Type-1A pilin)Potential fimbrial sfm operon (class γ1); Fimbrial Subunit. Disulfide bridge (41 81) PMID: 19820722 PMID: 20345943 PeriplasmicGN/AFimbriumPeriplasmicfimbriaeSECCUyesyesY230.832SpI2221.4596-00Yn6-17c22/23oExtracellular1083.3
FLGB_ECOLIP0ABW9
flgB

fla FII flbA

fla FII flbAb1073 JW1060 Flagellar basal-body rod protein flgB (Putative proximal rod protein)Periplasmic. No signal Peptide. Periplasmic PeriplasmProximal rod protein. PMID: 12730325 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 15136044PeriplasmicGN/ABacterial flagellum basal bodyPeriplasmic (Experimental)flagellumFlagellum FlayesyesN340.201CYT0-0.200913-000iPeriplasmic1093.85
FLGC_ECOLIP0ABX2
flgC

fla FIII flaW

fla FIII flaWb1074 JW1061 Flagellar basal-body rod protein flgC (Putative proximal rod protein)Periplasmic. No signal Peptide. Periplasmic Periplasm PeriplasmProximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGN/ABacterial flagellum basal bodyPeriplasmic (Experimental)flagellumFlagellum FlayesyesN320.201CYT0-0.200913-000iPeriplasmic1043.37
FLGG_ECOLIP0ABX5
flgG

fla FVII flaL

fla FVII flaLb1078 JW1065 Flagellar basal-body rod protein flgG (Distal rod protein)Periplasmic. No signal Peptide. Periplasmic Periplasm PeriplasmDistal rod protein PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGN/ABacterial flagellum basal bodyPeriplasmic (Experimental)flagellumFlagellum FlayesyesN240.123CYT0-0.200913-000iExtracellular1078.81
YBJC_ECOLIP46119
ybjC
b0850 JW0834 Uncharacterized protein ybjCIntegral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECyesyesN260.131TMH06.40394-220i12-29o44-68iCytoplasmicMembrane9.8245.74
YAJI_ECOLIP46122
yajI
b0412 JW5056 Uncharacterized lipoprotein yajIOuter Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane LipoproteinSECLOLnoyesY240.215SpII1918.9902Pos+2=V00Yn8-18c23/24oUnknown288.04
YFIM_ECOLIP46126
yfiM
b2586 JW2570 Uncharacterized protein yfiMContains the following signature IPR018736 Protein of unknown function DUF2279,Periplasmic lipoproteinInner Membrane LipoproteinEN/ACytoplasmicSECLOLyesyesY200.599SpII1415.2395Pos+2=S00Yn3-13c18/19oCytoplasmicMembrane9.8612.64
YGBE_ECOLIP46141
ygbE
b2749 JW2719 Inner membrane protein ygbEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.233TMH010.6872-330o23-46i53-79o85-106iCytoplasmicMembrane10
SURA_ECOLIP0ABZ6
surA
b0053 JW0052 Chaperone surA (Peptidyl-prolyl cis-trans isomerase surA) (PPIase surA) (EC 5.2.1.8) (Rotamase surA) (Survival protein A)Periplasmic PMID: 21370981 PMID: 19525348 PMID: 9298646 PMID: 22030350 PMID: 14506253PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY210.795SpI2015.5107-00Yn7-15c20/21oPeriplasmic1086.14
BAMD_ECOLIP0AC02
bamD

yfiO

yfiOb2595 JW2577 UPF0169 lipoprotein yfiOOuter membrane Lipoprotein Lipoprotein (experimental). Outer membrane protein. Outer membrane protein (experimental). Cell outer membrane Outer membrane protein (experimental).Part of bam complex. Forms subcomplex with BamCE. Outer membrane protein related to resistance to ampicillin and tetracycline. YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 16372265PMID: 16372265 PMID: 15174130 PMID: 16824102 PMID: 21823654 PMID: 21139201 PMID: 21370981 PMID: 12488465 PMID: 15911532 PMID: 16079137 PMID: 20932056Outer Membrane LipoproteinISoluble IM FractionCell outer membrane | Lipid-anchorOuter membrane Lipoprotein(experimental)SECLOLyesyesY240.171SpII1927.0165Pos+2=S00Yn7-17c22/23oOuterMembrane1022.46
FLIP_ECOLIP0AC05
fliP

flaR

flaRb1948 JW1932 Flagellar biosynthetic protein fliPPolytopic inner membrane. Cell inner membrane, Bacterial flagellum basal bodyA cleavable signal peptide is required for the full function of FliP protein.PMID: 15194505 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinBacterial flagellum basal bodyIntegral Membrane ProteinflagellumSRPSECnoyesY220.692TMH013.015-54Yn4-15c21/22o45-74i86-105o185-211i223-244oCytoplasmicMembrane10
FOCA_ECOLIP0AC23
focA

ycaE

ycaEb0904 JW0887 Probable formate transporter 1 (Formate channel 1)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN190.124TMH01.35944-660i35-55o75-98i118-141o161-180i192-212o255-277iCytoplasmicMembrane10
YGGM_ECOLIP46142
yggM
b2956 JW2923 Uncharacterized protein yggMPeriplasmicGN/APeriplasmicSECyesyesY220.874SpI2116.8631-00Yn5-16c21/22oPeriplasmic9.8432.42
RSEC_ECOLIP46187
rseC
b2570 JW2554 Sigma-E factor regulatory protein rseCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN250.187CYT0-0.200913-220i79-98o104-121iCytoplasmicMembrane1027.4
AAEX_ECOLIP46478
aaeX

yhcR

yhcRb3242 JW5541 Protein AaeXIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.106TMH010.7464-220o6-31i43-62oCytoplasmicMembrane9.8234.45
YQGD_ECOLIP46879
yqgD
b2941 JW2908 Uncharacterized protein yqgDIntegral Inner Membrane BBinoutout/inIM (predicted)Integral Membrane ProteinSRPSECpseudogene (Ecogene)nonoN620.118TMH01.01241-210o27-50iCytoplasmicMembrane9.82
NIRC_ECOLIP0AC26
nirC
b3367 JW3330 Probable nitrite transporterIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.428TMH04.72286-660i25-47o59-80i101-126o146-169i181-206o226-248iCytoplasmicMembrane10
FTSX_ECOLIP0AC30
ftsX

ftsS

ftsSb3462 JW3427 Cell division protein ftsXCell inner membrane Cell inner membrane Cell inner membrane Integral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 3323846 PMID: 10048040 PMID: 14729705 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN390.105CYT0-0.200913-440o72-95i214-240o272-290i323-342oCytoplasmicMembrane1023.24
DHSD_ECOLIP0AC44
sdhD
b0722 JW0712 Succinate dehydrogenase hydrophobic membrane anchor subunit. Alpha-helcal transmembrane protein (experimental). Integral inner membrane (experimental).PArt of colisuccinate dehydrogenase (SQR) composed of two hydrophilic subunits, a flavoprotein (SdhA) and iron-sulfur protein (SdhB) subunit, and two hydrophobic membrane anchor subunits, SdhC and SdhD.PMID: 11266632 PMID: 12560550 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN390.133TMH010.7371-330i17-39o59-80i92-113oCytoplasmicMembrane1044.37
KDTA_ECOLIP0AC75
waaA

kdtA

kdtAb3633 JW3608 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.4.-.-) (KDO transferase)Potential membrane association. Monotopic membrane protein.Involved in lipopolysaccharide biosynthesis PMID: 2651435 PMID: 1577828. Embedded in the cytoplasmic face of the inner membrane, WaaA catalyzes the transfer of 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) to the lipid A precursor of LPS (in Aquifex aeolicus) PMID: 22474366.PMID: 2651435 PMID: 1577828 PMID: 15866922 PMID: 22474366 PMID: 19275539Integral Inner Membrane BBinout/inIM (predicted)Cell inner membrane | Single-pass membrane proteinCytoplasmicSRPSECyesyesN640.121CYT0-0.200913-010o6-23iUnknown215.12
CSGC_ECOLIP52107
csgC
b1043 JW1026 Curli assembly protein CsgCUniprot mentions an 8aa signal peptide but Signal P prediction is for a "typical" 22 residue peptide Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PMID: 21645131 PMID: 19131513 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmicSECnoyesN220.179SpI222.07115-00Yn4-16c21/22oUnknown2.568.8
YPJK_ECOLIP52134
ypjK
b2635 JW5888 Uncharacterized protein ypjKIntegral Inner Membrane BBinoutout/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN460.265TMH05.94794-31Yn3-14c22/23o46-68iCytoplasmicMembrane10
YFJT_ECOLIP52135
yfjT
b2637 JW2618 Uncharacterized protein yfjTPeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoY240.673SpI2319.2157-00Yn9-19c23/24oUnknown2.522.23
HSLJ_ECOLIP52644
hslJ

ydbI

ydbIb1379 JW1374 Heat shock protein hslJOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLyesyesY250.238SpII1616.4623Pos+2=V00Yn4-15c20/21oUnknown2106.74
WECA_ECOLIP0AC78
wecA

rfe

rfeb3784 JW3758 Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.n2) (UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase) (Undecaprenyl-phosphate GlcNAc-1-phosphate transferase)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.115CYT0-0.200913-11110o6-24i45-66o72-89i101-119o131-149i161-179o185-206i213-232o244-263i294-315o321-342iCytoplasmicMembrane10
GNTP_ECOLIP0AC94
gntP

yjiB

yjiBb4321 JW4284 High-affinity gluconate transporter (Gluconate permease 3) (Gnt-III system)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN430.178TMH07.17039-13120i7-23o29-49i61-79o99-132i139-157o177-201i222-249o261-280i301-320o340-373i380-402o422-446iCytoplasmicMembrane10
GNTU_ECOLIP0AC96
gntU
b4476 JW5686 Low-affinity gluconate transporter (Gluconate permease) (Gnt-I system)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECpseudogene (Ochman & Davalos, 2006)yesyesN310.139TMH010.5274-13120i5-22o28-47i59-80o100-128i140-163o175-197i226-250o262-282i303-320o340-373i385-408o428-445iCytoplasmicMembrane10
HEMY_ECOLIP0ACB7
hemY
b3802 JW3774 Protein hemYIntegral inner membrane (experimental).Trigger factor-sensitive aggregation.PMID: 19737520 PMID: 15919996Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP,TFSECyesyesN230.235TMH010.5296-21Yn4-17c26/27o42-62iCytoplasmicMembrane1019.06
MBHM_ECOLIP0ACE0
hybC
b2994 JW2962 Hydrogenase-2 large chain (HYD2) (EC 1.12.99.6) (Membrane-bound hydrogenase 2 large subunit) (NiFe hydrogenase)Peripheral inner membrane protein facing the periplasm (experimental). Peripheral inner membrane protein facing the periplasm (experimental). Periplasmic The protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system piggyback. PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544PMID: 10224080 PMID: 3516690 PMID: 8772179 PMID: 9738917 PMID: 25664544Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane | Peripheral membrane proteinPeriplasmic (Experimental)TAT piggybackyesyesN340.139CYT0-0.200913-000iCytoplasmicMembrane1028.87
YJJZ_ECOLIP55914
yjjZ
b4567 JW5797 Uncharacterized protein yjjZIntegral Inner Membrane BBpolyin/outIM (predicted)Integral Membrane ProteinSRPSECyesyesN260.223TMH09.90172-330i7-25o31-48i55-75oCytoplasmicMembrane9.8219.7
HTRB_ECOLIP0ACV0
htrB

waaM

waaMb1054 JW1041 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) (Heat shock protein B)Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN200.104TMH01.65586-120i12-30o36-52iCytoplasmicMembrane1011.65
DDG_ECOLIP0ACV2
lpxP

ddg

ddgb2378 JW2375 Protein ddgExhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384Integral Inner Membrane BBinout/inIM (predicted)Cell inner membrane | Single-pass membrane proteinMembrane anchoredSRPYidCyesyesN490.166TMH01.68704-110o20-46iCytoplasmicMembrane9.829.41
LAPA_ECOLIP0ACV4
yciS
b1279 JW1271 Inner membrane protein yciSIntegral inner membrane (experimental). Heat shock protein involved in the assembly of LPS. In pull-down experiments, LapA and LapB co-purified with LPS, Lpt proteins, FtsH (protease), DnaK, and DnaJ (chaperones). A specific interaction was also observed between WaaC and LapB. LapB potentially coordinates assembly of proteins involved in LPS synthesis at the plasma membrane and regulates turnover of LpxC. PMID: 25403562 Heat shock protein involved in the assembly of LPS. In pull-down experiments, LapA and LapB co-purified with LPS, Lpt proteins, FtsH (protease), DnaK, and DnaJ (chaperones). PMID: 25403562PMID: 15919996 PMID: 25403562Integral Inner Membrane BBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.34TMH011.7404-21Yn4-16c21/22o45-67iCytoplasmicMembrane1036.3
YDGC_ECOLIP0ACX0
ydgC
b1607 JW1599 Inner membrane protein ydgCIntegral inner membrane (experimental). Dual topology membrane protein: can insert into the membrane in both directions. PMID: 15919996PMID: 15919996 PMID: 24688744 PMID: 16429150Integral Inner Membrane BBpoly, Bmultiin/out, out/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.169TMH03.73379-43Yn7-17c21/22o31-47i59-79o85-108iCytoplasmicMembrane1023.69
YEBS_ECOLIP0AD03
yebS
b1833 JW1822 Inner membrane protein yebSIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN570.112CYT0-0.200913-880i66-84o120-141i162-179o185-208i266-291o311-339i360-378o384-406iCytoplasmicMembrane10
BLR_ECOLIP56976
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b4409 JW5963 Beta-lactam resistance proteinCell inner membrane Cell inner membraneSmall membrane protein detected experimentally.PMID: 19121005 PMID: 10931331 PMID: 22885295Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN270.106CYT0-0.200913-110i7-26oCytoplasmicMembrane10
YMGF_ECOLIP58034
ymgF
b4520 JW1156 Uncharacterized protein ymgFCell inner membranePMID: 18978050Integral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECnoyesN370.129TMH00.559611-220i12-31o37-59iCytoplasmicMembrane9.8224.68
RZOD_ECOLIP58041
rzoD
b4510 JW5080 Outer membrane lipoprotein Rz1 from lambdoid prophage DLP12 Disulfide bridge (29 29) Outer Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLProphage Integrase (EcoGene)nonoY200.187SpII1919.9508Pos+2=T00Yn8-18c22/23oUnknown2147.46
RZOR_ECOLIP58042
rzoR
b4528 JW5213 Outer membrane lipoprotein Rz1 from lambdoid prophage Rac Disulfide bridge (29 29) Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane LipoproteinSECLOLProphage Integrase (EcoGene)nonoY200.189SpII1920.8844Pos+2=T00Yn8-18c22/23oUnknown288.14
YEEZ_ECOLIP0AD12
yeeZ
b2016 JW1998 Protein yeeZPotential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P].PMID: 18498364PeriplasmicGN/APeriplasmicSECSURAyesyesN250.197SpI246.50377-00Yn4-12c24/25oCytoplasmic8.9639.47
YEHU_ECOLIP0AD14
yehU
b2126 JW5353 Inner membrane protein yehUIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN220.144TMH06.36838-660i7-24o44-62i74-93o105-129i141-163o175-195iCytoplasmicMembrane102.15
YEJM_ECOLIP0AD27
yejM

yejN

yejNb2188 JW2176 Inner membrane protein yejMIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN680.133TMH014.6981-550i21-39o51-73i85-103o135-157i169-191oCytoplasmicMembrane102.24
YFCA_ECOLIP0AD30
yfcA
b2327 JW2324 UPF0721 transmembrane protein yfcAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN540.218TMH011.5476-780i7-28o53-73i85-105o111-127i139-158o164-183i195-217o237-255iCytoplasmicMembrane10
YFHB_ECOLIP0AD42
yfhB
b2560 JW5408 Uncharacterized protein yfhB Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 25403562 PMID: 25403562Integral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECYfgMnoyesN520.106CYT0-0.200913-110o36-54iCytoplasmicMembrane9.828.32
YOHJ_ECOLIP60632
yohJ
b2141 JW2129 UPF0299 membrane protein yohJIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN300.138TMH013.9988-440i12-32o38-55i67-86o92-113iCytoplasmicMembrane1015.71
YPFJ_ECOLIP64429
ypfJ
b2475 JW2460 Uncharacterized protein ypfJIntegral Inner Membrane BBoutin/outIM (predicted)Membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN330.101CYT0-0.200913-110i38-58oUnknown2
YBJM_ECOLIP64439
ybjM
b0848 JW0832 Inner membrane protein ybjMIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN340.187TMH012.0012-440i7-28o40-57i64-83o95-117iCytoplasmicMembrane1052.86
IVY_ECOLIP0AD59
ivy

ykfE

ykfEb0220 JW0210 Inhibitor of vertebrate lysozymePeriplasmic Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 11278658 PMID: 25664544PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECyesyesY290.849SpI2816.6514-00Yn13-23c28/29oPeriplasmic1027.17
PBP2_ECOLIP0AD65
mrdA

pbpA

pbpAb0635 JW0630 Penicillin-binding protein 2 (PBP-2)Inner membrane (experimental).PMID: 1103132 PMID: 15919657Integral Inner Membrane BBoutin/outIM (predicted), Soluble IM Fraction, Detected in IM FractionCell inner membrane | Peripheral membrane proteinMembrane anchoredSRPSECyesyesN370.174CYT0-0.200913-110i21-40oCytoplasmicMembrane9.828.4
FTSI_ECOLIP0AD68
ftsI

pbpB

pbpBb0084 JW0082 Peptidoglycan synthase ftsI (EC 2.4.1.129) (Penicillin-binding protein 3) (PBP-3) (Peptidoglycan glycosyltransferase 3)Septal ring. Inner membrane.The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein. Localizes to potential division sites in filamentous cells. Interacts directly with FtsN and ZapA. The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex.PMID: 11703663 PMID: 20497333 PMID: 20847002Integral Inner Membrane BBoutin/outSoluble IM FractionCell inner membrane | Single-pass membrane protein | Periplasmic sideMembrane anchoredSRPSECyesyesN370.141SpI453.45111-110i20-44oCytoplasmicMembrane9.9722.11
LIVJ_ECOLIP0AD96
livJ
b3460 JW3425 Leu/Ile/Val-binding protein (LIV-BP) Periplasmic Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 19953543 PMID: 9298646 PMID: 22030350 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.832SpI2320.6668-00Yn7-18c23/24oPeriplasmic1096.06
YCEO_ECOLIP64442
yceO
b1058 JW1045 Uncharacterized protein yceOSmall membrane protein detected experimentally.PMID: 19121005Integral Inner Membrane BBinoutin/outIM (predicted)Membrane anchoredSRPSECnoyesN160.097CYT0-0.200913-110i25-45oCytoplasmicMembrane9.82
YNAJ_ECOLIP64445
ynaJ
b1332 JW1326 Uncharacterized protein ynaJIntegral Inner Membrane BBoutin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN330.241TMH015.6597-21Yn15-26c31/32o55-80iCytoplasmicMembrane9.8229.56
YNBE_ECOLIP64448
ynbE
b1382 JW1377 Uncharacterized protein ynbEOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesN250.214SpII164.04646Pos+2=T00Yn4-15c24/25oUnknown2
BRNQ_ECOLIP0AD99
brnQ
b0401 JW0391 Branched-chain amino acid transport system 2 carrier protein (LIV-II)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN250.304TMH015.0757-12120i12-29o41-64i76-98o118-138i150-171o191-212i224-246o281-307i314-333o339-359i371-389o409-426iCytoplasmicMembrane10
TESA_ECOLIP0ADA1
tesA

apeA pldC

apeA pldCb0494 JW0483 Acyl-CoA thioesterase I (EC 3.1.2.-) (Lecithinase B) (Lysophospholipase L1) (EC 3.1.1.5) (Protease I)PeriplasmicPMID: 8098033PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesN270.484SpI2611.0906-10Yn12-22c26/27oPeriplasmic1040.74
NLPD_ECOLIP0ADA3
nlpD
b2742 JW2712 Lipoprotein nlpDOuter membrane Lipoprotein (experimental) Outer membrane protein (experimental).Proven signal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell inner membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY250.134SpII2521.9047Pos+2=S00Yn13-24c29/30oOuterMembrane9.9385.13
YAJG_ECOLIP0ADA5
yajG
b0434 JW0424 Uncharacterized lipoprotein yajGOuter membrane protein (experimental). Outer membrane protein (experimental). Outer membrane LipoproteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 9298646PMID: 15911532 PMID: 20932056 PMID: 9298646Outer Membrane LipoproteinISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY200.403SpII1719.0402Pos+2=A00Yn5-15c19/20oUnknown2.5
OSME_ECOLIP0ADB1
osmE

anr

anrb1739 JW1728 Osmotically-inducible lipoprotein E (Activator of ntr-like gene protein)Lipoprotein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane Lipoprotein Proven signal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 21541338 PMID: 10806384 PMID: 25664544Outer Membrane LipoproteinISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY240.207SpII2020.0226Pos+2=T10Yn7-18c23/24oUnknown298.73
YDCX_ECOLIP64453
ydcX
b1445 JW5232 Uncharacterized protein ydcXIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN190.119TMH010.662-220o6-25i37-56oCytoplasmicMembrane9.82
YNCJ_ECOLIP64459
yncJ
b1436 JW1432 Uncharacterized protein yncJPeriplasmicGN/APeriplasmicSECnoyesY230.752SpI228.63896-00Yn5-16c22/23oUnknown2.538.04
YDHI_ECOLIP64471
ydhI
b1643 JW1635 Uncharacterized protein ydhIIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN320.128TMH010.2168-220o20-44i56-77oCytoplasmicMembrane9.8243.86
ECNB_ECOLIP0ADB7
ecnB

yjeU

yjeUb4411 JW4108 Entericidin BOuter membrane Lipoprotein (experimental). Outer membrane protein (experimental). Potentially toxic protein. Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130PMID: 15174130 PMID: 19121005 PMID: 20932056 PMID: 21541338Outer Membrane LipoproteinISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY220.27SpII2117.5085Pos+2=N10Yn5-15c20/21oCytoplasmicMembrane10
LPTE_ECOLIP0ADC1
lptE

rlpB

rlpBb0641 JW0636 LPS-assembly lipoprotein lptE (Rare lipoprotein B)Lipoprotein (experimental). Outer membrane protein (experimental). Outer membrane Lipoprotein Proven signal peptidase II substrate. PMID: 16861298PMID: 15174130 PMID: 20932056 PMID: 16861298 PMID: 22901060Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN250.125SpII1817.3262Pos+2=G00Yn4-15c20/21oOuterMembrane1080.93
LOLC_ECOLIP0ADC3
lolC

ycfU

ycfUb1116 JW5161 Lipoprotein-releasing system transmembrane protein lolCIntegral Membrane ProteinPMID: 15276320 PMID: 10783239Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN190.179TMH02.37377-450o25-48i269-290o310-336i343-360o366-385iCytoplasmicMembrane1029.76
LPTG_ECOLIP0ADC6
lptG

yjgQ

yjgQb4262 JW5760 Lipopolysaccharide export system permease protein lptGIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN360.105TMH04.7678-660i12-33o66-84i105-125o279-298i305-322o334-356iCytoplasmicMembrane109.06
YJJP_ECOLIP0ADD5
yjjP
b4364 JW5796 Inner membrane protein yjjPIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN280.178CYT0-0.200913-350i114-135o141-158i170-189o195-214i226-252oCytoplasmicMembrane1016.1
YDJM_ECOLIP64481
ydjM
b1728 JW5281 Inner membrane protein ydjMIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN280.333CYT0-0.200913-04Yn7-17c22/23o32-48i69-90o110-128i149-166oCytoplasmicMembrane1015.67
YEAQ_ECOLIP64485
yeaQ
b1795 JW1784 UPF0410 protein yeaQIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN180.108TMH015.2932-330o6-21i28-52o58-77iCytoplasmicMembrane10
YOAF_ECOLIP64493
yoaF
b1793 JW1782 Uncharacterized protein yoaFOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesY240.245SpII1620.7594Pos+2=S00Yn4-15c23/24oUnknown290.73
YEBO_ECOLIP64499
yebO
b1825 JW1814 Uncharacterized protein yebOIntegral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN360.143CYT0-0.200913-110o12-32iUnknown246.45
TAMA_ECOLIP0ADE4
tamA

yftM ytfM

yftM ytfMb4220 JW4179 Translocation and assembly module TamAOuter Membrane Outer MembranePart of the translocation and assembly module (TAM). TAM system promotes efficient secretion of autotransporters in proteobacteria.PMID: 17214547 PMID: 22466966Outer Membrane b-barrel proteinHN/AOuter Membrane β-barrel proteinSECBAMyesyesY220.861SpI2111.5811-00Yn7-15c21/22oOuterMembrane10
YGFZ_ECOLIP0ADE8
ygfZ

yzzW

yzzWb2898 JW2866 tRNA-modifying protein ygfZPeriplasmic CytoplasmicSubcellular localization analysis of cytotoxic necrotizing factor 1 (CNF1) demonstrated that YgfZ, a periplasmic protein, contributes to secretion of CNF1 (a normally cytoplasmic protein) into outer-membrane vesicles (OMVs) in pathogenic E.coli strain RS218 O18:K1:H7 PMID: 22174383. PMID: 9298646PMID: 22174383 PMID: 9298646Cytoplasmic, PeriplasmicA, GN/ACytoplasmCytoplasmic (Experimental)SEC, non classicalyesyesN310.252CYT0-0.200913-000iUnknown282.19
YIAF_ECOLIP0ADK0
yiaF
b3554 JW5655 Uncharacterized protein yiaFUncharacterized protein yiaF - echolocation Occurrence of signal peptide determined by LipoP signal sequence shown based upon lipoP, not possible to determine which membrane based on +2 amino acid rule.Inner Membrane LipoproteinESoluble IM FractionMembrane LipoproteinSECLOLyesyesN280.132SpII2510.3533Pos+2=F10Yn10-21c26/27oUnknown2.567.16
NEPI_ECOLIP0ADL1
nepI

yicM

yicMb3662 JW5938 Purine ribonucleoside efflux pump nepIIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN480.151TMH08.80109-12120i21-44o56-75i87-106o112-133i145-164o176-196i217-239o254-275i282-301o307-329i341-363o369-389iCytoplasmicMembrane10
YEBY_ECOLIP64506
yebY
b1839 JW1828 Uncharacterized protein yebY Identified DsbA substrate PMID: 19953543PMID: 19953543PeriplasmicGN/APeriplasmicSECDSBAyesyesY210.862SpI2019.6801-10Yn5-12c20/21oUnknown2.535.83
MGRB_ECOLIP64512
mgrB

yobG

yobGb1826 JW1815 Protein mgrBSmall membrane protein detected experimentally. Localization and bacterial two-hybrid studies suggest that MgrB resides in the inner-membrane and interacts directly with PhoQ.PMID: 19121005 PMID: 20041203Integral Inner Membrane BBinoutin/outN/APeriplasmicSECnoyesN220.24SpI2110.2239-10Yn6-17c21/22oCytoplasmicMembrane9.8653.79
YOHN_ECOLIP64534
rcnB

yohN

yohNb2107 JW5346 Uncharacterized protein yehEPeriplasmPMID: 21665978PeriplasmicGN/APeriplasmicSECnoyesY220.81SpI2123.7077-10Yn8-16c21/22oUnknown2.579.93
YEIS_ECOLIP64536
yeiS
b2145 JW5359 Uncharacterized protein yeiSIntegral Inner Membrane B-IM (predicted)Membrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN240.124TMH02.78571-220o6-24i54-77oCytoplasmicMembrane9.8257.44
YIGM_ECOLIP0ADP5
yigM
b3827 JW3803 Uncharacterized membrane protein yigMIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN200.164TMH06.74192-109Yn2-13c19/20o29-47i59-81o87-105i112-130o136-158i170-190o202-222i234-253o259-276iCytoplasmicMembrane10
YIID_ECOLIP0ADQ2
yiiD
b3888 JW3859 Uncharacterized protein yiiDCopurifies with trigger factor in pull down assays.PMID: 19737520Integral Inner Membrane BBinoutout/inIM (predicted)CytoplasmicSRP,TFSECnoyesN290.101CYT0-0.200913-010o219-240iCytoplasmic8.9653.85
YQAA_ECOLIP0ADR0
yqaA
b2689 JW2664 Inner membrane protein yqaAIntegral inner membrane (experimental).Member of the DedA family. DedA family proteins may play a more general role in membrane protein function or quality control and in maintenance of the PMF. PMID: 15919996PMID: 15919996 PMID: 23086209Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN190.233TMH05.40517-43Yn4-12c17/18o41-65i86-110o116-141iCytoplasmicMembrane104.67
YGGN_ECOLIP0ADS9
yggN
b2958 JW2925 Uncharacterized protein yggNPeriplasmic Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535PMID: 14726535 PMID: 19953543PeriplasmicGN/APeriplasmic(experimental)SECDSBAyesyesY210.797SpI2021.2471-00Yn6-16c20/21oUnknown2.580.23
YGIM_ECOLIP0ADT8
ygiM
b3055 JW3027 Uncharacterized protein ygiMIntegral Inner Membrane BBinoutout/inIM (predicted), Detected in IM FractionMembrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesY230.542SpI2218.2018-11Yn6-17c22/23o173-195iUnknown2.512.61
LPTC_ECOLIP0ADV9
lptC

yrbK

yrbKb3199 JW3166 Lipopolysaccharide export system protein lptCCell inner membrane Cell inner membraneEcholocation says Signal peptide may be membrane anchored or cleaved and the protein therefore exported (signalP score near threshold)PMID: 18424520 PMID: 20720015Integral Inner Membrane BBoutin/outSoluble IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN260.212SpI257.46611-10Yn7-18c27/28oUnknown251.41
YPEC_ECOLIP64542
ypeC
b2390 JW2387 Uncharacterized protein ypeCPeriplasmicGN/APeriplasmicSECnoyesY220.729SpI2114.9819-00Yn4-15c21/22oUnknown2.514.14
YFGG_ECOLIP64545
yfgG
b2504 JW5399 Uncharacterized protein yfgGIntegral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN410.141TMH03.8997-10Yn20-31c39/40oCytoplasmicMembrane9.82
YFIR_ECOLIP64548
yfiR
b2603 JW2584 Uncharacterized protein yfiR Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY230.684SpI2213.4131-00Yn7-17c22/23oUnknown6.5821.98
AlaE_ECOLIP64550
alaE

ygaW

ygaWb2670 JW2645 Uncharacterized protein ygaWCell inner membranePMID: 21531828Integral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN490.117TMH06.24909-440i12-36o42-62i82-104o110-131iCytoplasmicMembrane1012.19
YHCB_ECOLIP0ADW3
yhcB
b3233 JW5539 Putative cytochrome d ubiquinol oxidase subunit 3 (EC 1.10.3.-) (Cytochrome bd-I oxidase subunit III)Inner membrane (experimental). Inner membraneHas been isolated from inner membrane preparations in a complex with one subunit each of CydA and CydB, suggesting that YhcB is in fact a subunit of the cytochrome bd ubiquinol oxidase complex. YhcB was demonstrated to interact with RodZ as well as several other proteins involved in cell shape maintenance and an inner membrane protein YciS of unknown function, using bacterial two-hybrid system.These observations seem to indicate that YhcB is involved in the biogenesis of cell envelope and the maintenance of cell shape together with RodZ. PMID: 16079137 PMID: 21210718 PMID: 15919657 Binout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN210.134CYT0-0.200913-110o6-24iCytoplasmicMembrane9.8283.09
PPID_ECOLIP0ADY1
ppiD

ybaU

ybaUb0441 JW0431 Peptidyl-prolyl cis-trans isomerase D (PPIase D) (EC 5.2.1.8) (Rotamase D)Inner membrane, Periplasmic side Membrane anchored By in vivo and in vitro site-directed cross-linking, it has been demostrated that the periplasmic chaperone PpiD is located in front of the lateral gate of SecY, through which transmembrane domains exit the SecY channel. Blue native PAGE analyses verify the presence of a SecYEG-PpiD complex in native Escherichia coli membranes. PMID: 24951590 Consistent with a coupling between translocation across the SecYEG translocon and folding by periplasmic chaperones, a lack of PpiD retards the release of a translocating outer membrane protein into the periplasm. PMID: 18439025PMID: 16079137 PMID: 9670013 PMID: 24951590 PMID: 18439025Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionCell inner membrane | Single-pass type II membrane protein | Periplasmic sideMembrane anchored (Experimental)SRPSECyesyesN430.199TMH03.55242-110i12-35oCytoplasmicMembrane1052.9
YAJC_ECOLIP0ADZ7
yajC
b0407 JW0397 UPF0092 membrane protein yajCCell inner membrane Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 16079137 PMID: 21541338Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN170.156TMH0-0.000234246-110o20-39iCytoplasmicMembrane1041.19
ACRA_ECOLIP0AE06
acrA

lir mtcA

lir mtcAb0463 JW0452 Acriflavine resistance protein ALipoprotein (experimental). Inner membrane. Cell inner membrane. Inner membrane lipoproteinProven signal peptidase II substrate. PMID: 10894736PMID: 15174130 PMID: 15228545 PMID: 16079137 PMID: 10894736Inner Membrane LipoproteinESoluble IM FractionCell inner membrane | Lipid-anchorInner membrane lipoprotein (Experimental)SECLOLyesyesY220.194SpII2419.636Pos+2=D00Yn6-16c21/22oCytoplasmicMembrane1064.7
YGGT_ECOLIP64564
yggT
b2952 JW2919 Uncharacterized protein yggTIntegral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECYfgMyesyesN310.194TMH00.565687-440o6-25i67-91o97-119i144-165oCytoplasmicMembrane105.26
YQJE_ECOLIP64585
yqjE
b3099 JW3070 Inner membrane protein yqjEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN620.126CYT0-0.200913-220i50-76o82-104iCytoplasmicMembrane10
AMPE_ECOLIP0AE14
ampE
b0111 JW0107 Protein AmpEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN230.115CYT0-0.200913-44Yn2-12c16/17o40-58i65-85o148-168i266-283oCytoplasmicMembrane103.49
AMPG_ECOLIP0AE16
ampG
b0433 JW0423 Protein AmpGIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN380.305TMH012.6077-14140i15-38o44-61i82-101o107-131i143-165o177-195i225-250o262-281i288-309o321-342i354-376o382-402i423-445o465-485iCytoplasmicMembrane10
APHA_ECOLIP0AE22
aphA

napA yjbP

napA yjbPb4055 JW4015 Class B acid phosphatase (EC 3.1.3.2)Periplasm PeriplasmicPMID: 9011040 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY260.643SpI2514.5914-00Yn7-17c25/26oUnknown2.5
ARAE_ECOLIP0AE24
araE
b2841 JW2809 Arabinose-proton symporter (Arabinose transporter)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN450.136TMH07.37397-12120i23-51o63-84i91-110o116-137i149-167o179-198i258-281o297-317i329-348o360-384i405-422o428-446iCytoplasmicMembrane10
ARAH_ECOLIP0AE26
araH
b4460 JW1887 L-arabinose transport system permease protein AraHIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCpseudogene (Ochman & Davalos, 2006)noyesN470.147TMH013.0464-9100i21-42o54-72i79-96o102-124i131-150o170-191i222-240o252-269i276-295o301-317iCytoplasmicMembrane10
YHAH_ECOLIP64590
yhaH
b3103 JW3074 Inner membrane protein yhaHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.122TMH08.08221-330o23-40i52-70o82-103iCytoplasmicMembrane108.9
YHAI_ECOLIP64592
yhaI
b3104 JW3075 Inner membrane protein yhaIIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.264TMH00.961473-330i23-44o50-68i80-98oCytoplasmicMembrane1028.42
YHCN_ECOLIP64614
yhcN
b3238 JW5540 Uncharacterized protein yhcNPeriplasmicGN/APeriplasmPeriplasmicSECyesyesY230.834SpI2222.7033-00Yn7-18c22/23oUnknown2.5
ARTM_ECOLIP0AE30
artM
b0861 JW0845 Arginine ABC transporter permease protein ArtMIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN270.106TMH012.0162-550o20-39i51-70o82-100i155-176o188-211iCytoplasmicMembrane10
ARTQ_ECOLIP0AE34
artQ
b0862 JW0846 Arginine ABC transporter permease protein ArtQIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN300.173TMH017.1037-450o12-35i42-68o101-118i161-182o202-223iCytoplasmicMembrane1026.25
YTFL_ECOLIP0AE45
ytfL
b4218 JW4177 UPF0053 inner membrane protein ytfLIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.622SpI269.49148-43Yn5-18c26/27o56-80i100-119o139-162iCytoplasmicMembrane1048.28
CITT_ECOLIP0AE74
citT

ybdS

ybdSb0612 JW0604 Citrate carrier (Citrate transporter) (Citrate/succinate antiporter)Integral Membrane ProteinPMID: 9696764Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN580.164TMH013.6692-13140i7-27o33-50i57-74o94-117i138-155o161-178i190-213o233-259i288-304o310-329i341-361o381-412i424-443o463-486iCytoplasmicMembrane10
CPXA_ECOLIP0AE82
cpxA

ecfB eup ssd

ecfB eup ssdb3911 JW3882 Sensor protein CpxA (EC 2.7.13.3)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN190.111TMH01.20569-220o6-27i165-184oCytoplasmicMembrane1016.7
YHDU_ECOLIP64619
yhdU
b3263 JW3231 Uncharacterized protein yhdUPotential Signal PeptideIntegral Inner Membrane BBinoutin/outIM (predicted)Membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN310.117TMH00.197917-10Yn7-18c30/31oCytoplasmicMembrane9.8282.37
YHDV_ECOLIP64622
yhdV
b3267 JW3235 Uncharacterized protein yhdVOuter membrane Lipoprotein (experimental).Proven signal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIIM (predicted)Cell membrane | Multi-pass membrane proteinOuter membrane Lipoprotein (Experimental)SECLOLnoyesY210.268SpII1614.2179Pos+2=A11Yn4-15c20/21o50-71iUnknown2.530.35
CPXP_ECOLIP0AE85
cpxP

yiiO

yiiOb4484 JW5558 Periplasmic protein CpxPPeriplasmic. Membrane associated PeriplasmNovel bacterial periplasmic protein with no homologues of known function. CpxP is thought to interact with the two-component sensor kinase, CpxA, to inhibit induction of the Cpx envelope stress response in the absence of protein misfolding. CpxP has also been shown to facilitate DegP-mediated proteolysis of misfolded proteins. PMID: 21317318PMID: 17904518 PMID: 10972835 PMID: 21317318Peripheral inner membrane protein facing the periplasmF2N/APeriplasmMembrane associated (Experimental)SECpseudogene (Ochman & Davalos, 2006)yesyesY220.806SpI2116.0374-00Yn3-14c21/22oPeriplasmic10104.98
CSGE_ECOLIP0AE95
csgE
b1039 JW1022 Curli production assembly/transport component csgEPeriplasmicPotential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475PMID: 16704339 PMID: 18498364 PMID: 21645131 PMID: 19131513 PMID: 9928475PeriplasmicGN/APeriplasmic (Experimental)CurliSECSURAyesyesY230.789SpI223.89784-00Yn7-17c22/23oUnknown215.08
CSGF_ECOLIP0AE98
csgF
b1038 JW1021 Curli production assembly/transport component csgFOuter membrane protein. Peripheral outer membrane protein facing the periplasm. Peripheral outer membrane protein facing the periplasm. Cell surface appendage PeriplasmicCurli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475PMID: 16704339 PMID: 16522795 PMID: 21645131 PMID: 19131513 PMID: 19131513 PMID: 9928475Peripheral outer membrane protein facing the extra-cellular spaceF4N/APeriplasmic (Experimental)CurliSECCurliyesyesY200.853SpI1913.9164-10Yn6-14c19/20oExtracellular9.6430.3
CSGG_ECOLIP0AEA2
csgG
b1037 JW1020 Curli production assembly/transport component csgGOuter membrane Lipoprotein (experimental)Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130PMID: 16704339 PMID: 15174130 PMID: 21645131 PMID: 16420357 PMID: 19131513Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceI, F4N/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)CurliSECLOLyesyesY200.544SpII1512.9163Pos+2=L00Yn4-13c18/19oCytoplasmicMembrane9.8244.19
YHFL_ECOLIP64627
yhfL
b3369 JW3332 Uncharacterized protein yhfLOuter membrane Lipoprotein (experimental). Inner membrane. Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 21890895Outer Membrane LipoproteinIN/AMembrane | Single-pass membrane proteinOuter membrane Lipoprotein (Experimental)SECLOLnoyesN220.162SpII1915.9797Pos+2=T00Yn7-17c22/23oUnknown2
YHFU_ECOLIP64631
yhfU
b3378 JW5697 Uncharacterized protein yhfUIntegral Inner Membrane BBinoutout/inN/AMembrane anchoredSRPSECnoyesN370.114CYT0-0.200913-110o47-69iUnknown290.44
YRFC_ECOLIP64634
hofN

yrfC

yrfCb3394 JW3357 Uncharacterized protein yrfCIntegral Inner Membrane BBoutin/outN/AMembrane anchoredSRPSECyesyesN490.175TMH03.72613-110i21-40oCytoplasmicMembrane9.8222.14
YJDO_ECOLIP64646
yjdO

yjdO

yjdOb4559 JW5732 Uncharacterized protein yjdOToxin-antitoxin system Ghot-GhoS.In vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA.PMID: 22941047Integral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN330.1TMH09.94204-220i7-26o32-56iCytoplasmicMembrane9.82
CYOE_ECOLIP0AEA5
cyoE
b0428 JW0418 Protoheme IX farnesyltransferase (EC 2.5.1.-) (Heme B farnesyltransferase) (Heme O synthase)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN580.11TMH02.59686-990i12-30o36-60i80-102o108-127i134-156o162-183i209-227o233-252i264-285oCytoplasmicMembrane10
CYSW_ECOLIP0AEB0
cysW
b2423 JW2416 Sulfate transport system permease protein cysWIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.154TMH013.7791-660i20-43o63-89i101-124o144-162i202-228o248-268iCytoplasmicMembrane1052.07
DACA_ECOLIP0AEB2
dacA

pfv

pfvb0632 JW0627 D-alanyl-D-alanine carboxypeptidase dacA (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Beta-lactamase) (EC 3.5.2.6) (Penicillin-binding protein 5) (PBP-5)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Membrane anchored Membrane anchored Peripheral inner membrane protein facing the periplasm.Anchored to inner membrane by C-terminal tailPMID: 2194801. Using natural isotope abundance NMR, the structure of the PBP 5 anchor peptide within a micelle was determinedPMID: 20192190. The structure conforms to a helix-bend-helix-turn-helix motif and reveals that the anchor enters the membrane so as to form an amphiphilic structure within the interface of the hydrophilic/hydrophobic boundary regions near the lipid head groups. PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones PMID: 20545860. PMID: 3279397 PMID: 8424800 DacA(PBP-5) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. A pH dependent loss of alpha-helical conformation in the peptide corresponding to the PBP5 C-terminus was found to correlate with the susceptibility of PBP5 to membrane extraction. PMID: 9371419PMID: 2194801 PMID: 20192190 PMID: 20545860 PMID: 24057063 PMID: 3279397 PMID: 8424800 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideMembrane anchored (Experimental)SECyesyesY300.737SpI2917.6151-00Yn13-24c29/30oCytoplasmicMembrane9.978.45
YFGH_ECOLIP65290
yfgH
b2505 JW5400 Uncharacterized lipoprotein yfgHLipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY270.349SpII2124.716Pos+2=Q00Yn7-17c22/23oUnknown2.573.25
YGDR_ECOLIP65294
ygdR
b2833 JW2801 Uncharacterized lipoprotein ygdROuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesN280.252SpII1915.677Pos+2=S10Yn6-17c22/23oUnknown2.5
YQHH_ECOLIP65298
yqhH
b3014 JW2982 Uncharacterized lipoprotein yqhHOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN240.242SpII1915.1531Pos+2=V10Yn4-17c22/23oOuterMembrane9.9317.87
YHGN_ECOLIP67143
yhgN
b3434 JW3397 UPF0056 inner membrane protein yhgNIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN260.114CYT0-0.200913-660o6-27i39-57o63-85i106-127o139-162i174-195oCytoplasmicMembrane1037.49
YNAI_ECOLIP0AEB5
ynaI
b1330 JW1323 MscS family inner membrane protein ynaICell inner membrane Integral inner membrane (experimental).PMID: 22874652 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN220.127TMH07.65863-450o12-30i42-65o77-96i117-134o140-159iCytoplasmicMembrane1014.54
YOAE_ECOLIP0AEC0
yoaE
b1816 JW1805 UPF0053 inner membrane protein yoaEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.115TMH01.99313-770o12-36i48-69o81-101i122-148o154-172i184-207o213-231iCytoplasmicMembrane1010.84
ARCB_ECOLIP0AEC3
arcB
b3210 JW5536 Aerobic respiration control sensor protein ArcB (EC 2.7.13.3)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN360.18TMH09.51619-220o20-46i58-77oCytoplasmicMembrane10
BARA_ECOLIP0AEC5
barA
b2786 JW2757 Signal transduction histidine-protein kinase BarA (EC 2.7.13.3)Cell inner membraneExhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN340.123TMH07.64299-220o12-31i177-199oCytoplasmicMembrane1056.26
YQHA_ECOLIP67244
yqhA
b3002 JW2971 UPF0114 protein yqhAIntegral Membrane ProteinPMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN320.15TMH06.53453-340o12-32i53-75o109-126i138-156oCytoplasmicMembrane1015.29
DCUS_ECOLIP0AEC8
dcuS

yjdH

yjdHb4125 JW4086 Uncharacterized protein yjeNIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN480.105TMH05.80308-220i21-41o183-205iCytoplasmicMembrane1035.3
DCRB_ECOLIP0AEE1
dcrB

yhhR

yhhRb3472 JW5682 Protein dcrBOuter membrane protein (experimental).PMID: 12558182 PMID: 15911532Inner Membrane LipoproteinESoluble IM FractionPeriplasmInner membrane lipoproteinSECLOLyesyesN300.169SpII1921.1Pos+2=D00Yn8-15c20/21oPeriplasmic10100.37
DEGS_ECOLIP0AEE3
degS

hhoB htrH

hhoB htrHb3235 JW3204 Protease degS (EC 3.4.21.-)Inner membrane anchored. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428PMID: 11442831 PMID: 17360428PeriplasmicGSoluble IM FractionPeriplasmMembrane anchoredSECyesyesN280.167SpI284.49468-10Yn9-20c28/29oPeriplasmic9.4475.55
DGAL_ECOLIP0AEE5
mglB
b2150 JW2137 Autoinducer 2 import ATP-binding protein lsrA (AI-2 import ATP-binding protein lsrA) (EC 3.6.3.-) (EGO10A) Periplasmic Periplasmic Potential Skp substrate PMID: 19953543 PMID: 7012152 PMID: 9298646PMID: 19953543 PMID: 7012152 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSKPnoyesY240.861SpI2323.7963-10Yn5-16c23/24oPeriplasmic1075.23
YOBD_ECOLIP67601
yobD
b1820 JW1809 UPF0266 membrane protein yobDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.285TMH03.32601-230o6-24i45-62o68-87iCytoplasmicMembrane10
MDTI_ECOLIP69210
mdtI

ydgE

ydgEb1599 JW1591 Spermidine export protein mdtIIntegral inner membrane (experimental).PMID: 15919996 PMID: 24688744Integral Inner Membrane BBpoly, Bmultiout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN560.185TMH03.45748-440o6-25i37-56o62-84i91-108oCytoplasmicMembrane1014.92
MDTJ_ECOLIP69212
mdtJ

ydgF

ydgFb1600 JW1592 Spermidine export protein mdtJIntegral inner membrane (experimental).PMID: 15919996 PMID: 24688744Integral Inner Membrane BBpoly, Bmultiout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN260.125TMH07.68505-43Yn3-13c21/22o31-52i59-80o86-102iCytoplasmicMembrane1036.97
DPPB_ECOLIP0AEF8
dppB
b3543 JW3512 Dipeptide transport system permease protein dppBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.168TMH06.13051-660i9-30o100-123i135-160o201-221i259-285o305-331iCytoplasmicMembrane10
DPPC_ECOLIP0AEG1
dppC
b3542 JW3511 Dipeptide transport system permease protein dppCIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN590.13TMH00.391748-550o32-53i102-127o147-173i215-244o264-287iCytoplasmicMembrane104.14
DSBA_ECOLIP0AEG4
dsbA

dsf ppfA

dsf ppfAb3860 JW3832 Thiol:disulfide interchange protein DsbAPeriplasmic Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential β-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544PMID: 18406599 PMID: 20615876 PMID: 19525348 PMID: 13129941 PMID: 15838024 PMID: 9298646 PMID: 15838024 PMID: 13129941 PMID: 9300489 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SRP (exp)SECyesyesY200.75SpI1916.7987-00Yn4-15c19/20oPeriplasmic1071.65
DSBC_ECOLIP0AEG6
dsbC

xprA

xprAb2893 JW2861 Thiol:disulfide interchange protein DsbCPeriplasm Periplasmic Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential β-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544PMID: 18406599 PMID: 20615876 PMID: 8168498 PMID: 9298646 PMID: 10700276 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY210.778SpI2021.5074-00Yn4-15c20/21oPeriplasmic1082.33
PGAD_ECOLIP69432
pgaD

ycdP

ycdPb1021 JW1006 Biofilm PGA synthesis protein PgaDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN380.135TMH010.5144-220i16-36o56-74iCytoplasmicMembrane109.97
PTXB_ECOLIP69789
glvB

glvC

glvCb3682 JW3659 Phosphotransferase enzyme IIB component glvB (EC 2.7.1.69) (PTS system EIIB component)Integral Inner Membrane BBinout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinMembrane anchoredSRPSECpseudogene (Ochman & Davalos, 2006)yesnoN450.17CYT0-0.200913-110o12-31iCytoplasmicMembrane9.8233.58
PTFB1_ECOLIP69808
fryB

ypdH

ypdHb2387 JW5389 Fructose-like phosphotransferase enzyme IIB component 1 (EC 2.7.1.69) (PTS system fructose-like EIIB component 1)PeriplasmicGN/ACytoplasmPeriplasmicSECyesyesY250.406SpI2111.3983-00Yn5-16c24/25oUnknown4.9994.5
NANC_ECOLIP69856
nanC

yjhA

yjhAb4311 JW5778 Probable N-acetylneuraminic acid outer membrane channel protein nanC (NanR-regulated channel) (Porin nanC)PeriplasmicGN/ACell outer membranePeriplasmicSECnoyesY240.708SpI2319.1942-00Yn6-15c23/24oOuterMembrane108.83
RSEP_ECOLIP0AEH1
rseP

ecfE yaeL

ecfE yaeLb0176 JW0171 Regulator of sigma E protease (EC 3.4.24.-)Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membraneFluorescent fusions of RseP have been monitored via fluorescence microscopy. RseP has been found to generally associated with the membrane.PMID: 17272300 PMID: 11689431 PMID: 11750129 PMID: 11867724 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN330.109TMH03.00034-440o6-29i98-119o376-396i426-444oCytoplasmicMembrane1016.14
ELAB_ECOLIP0AEH5
elaB

yfbD

yfbDb2266 JW2261 Protein elaBRibosomeElaB is an YqjD paralog and also has transmembrane motif at the C-terminal region. ElaB is an YqjD paralog and also has transmembrane motif at the C-terminal region. Probably binds to the membrane and ribosomes in a manner similar to that of YqjD PMID: 22661687.PMID: 22661687Integral Inner Membrane BBoutout/inIM (predicted), Detected in IM FractionMembrane anchoredSRPSECyesyesN330.125CYT0-0.200913-110o81-99iUnknown299.87
EMRB_ECOLIP0AEJ0
emrB
b2686 JW2661 Multidrug resistance protein BIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 15919996PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPYidCyesyesN360.208TMH05.07213-13130i12-33o53-72i81-100o106-128i140-161o167-189i201-220o232-251i272-291o303-324i336-353o365-390i480-498oCytoplasmicMembrane10
ENVZ_ECOLIP0AEJ4
envZ

ompB perA tpo

ompB perA tpob3404 JW3367 Osmolarity sensor protein envZ (EC 2.7.13.3)Integral inner membrane (experimental).Contains HAMP an intrinsically disordered domain. EnvZ HAMP domain by itself is unable to form a stable structure and that this structural fragility may be important for its role in signal transduction. PMID: 2668953 PMID: 15522865 PMID: 17635923 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.263TMH07.11037-220i12-39o160-179iCytoplasmicMembrane10
YAAX_ECOLIP75616
yaaX
b0005 JW0004 Uncharacterized protein yaaXPeriplasmicGN/APeriplasmicSECnoyesY240.841SpI2319.693-10Yn6-17c23/24oUnknown2.577.96
FBPB_ECOLIP75681
fbpB

afuB

afuBb0263 JW0255 Putative ferric transport system permease protein fbpB (Fragment)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN460.154CYT0-0.200913-220i33-56o76-101iCytoplasmicMembrane9.8230.56
YKGB_ECOLIP75685
ykgB
b0301 JW5038 Inner membrane protein ykgBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.117TMH00.0510112-340i12-32o87-105i112-133o153-175iCytoplasmicMembrane10
FDNI_ECOLIP0AEK7
fdnI
b1476 JW1472 Formate dehydrogenase, nitrate-inducible, cytochrome b556(fdn) subunit (Anaerobic formate dehydrogenase cytochrome b556 subunit) (Formate dehydrogenase-N subunit gamma) (FDH-N subunit gamma) Alpha-helcal transmembrane protein (experimental). Transmembrane helices: 3. Integral inner membrane (experimental). PMID: 15342602 PMID: 11266632 PMID: 11884747 PMID: 15919996 PMID: 11929547Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN370.156TMH022.1464-440i21-43o55-76i117-138o150-174iCytoplasmicMembrane1035.28
FDOI_ECOLIP0AEL0
fdoI
b3892 JW3863 Formate dehydrogenase, cytochrome b556(fdo) subunit (Aerobic formate dehydrogenase cytochrome b556 subunit) (FDH-Z subunit gamma) (Formate dehydrogenase-O subunit gamma)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.115TMH016.4965-440i20-43o55-73i113-133o145-172iCytoplasmicMembrane1016.96
FEPB_ECOLIP0AEL6
fepB
b0592 JW0584 Ferrienterobactin-binding periplasmic proteinPeriplasmicPMID: 10986237PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.581SpI2613.3736-00Yn10-21c26/27oPeriplasmic1030.41
FLGM_ECOLIP0AEM4
flgM
b1071 JW1058 Negative regulator of flagellin synthesis (Anti-sigma-28 factor)Extra-cellular.Anti-sigma28 factor. FlgM is an anti-sigma factor that binds and inactivates sigma28 (FliA_Ecoli). Once the hook and basal body is assembled, FlgM is secreted by the flagellar export system thereby freeing sigma28 to activate transcription.PMID: 10322161 PMID: 12631703 PMID: 9988479Cytoplasmic, Extra-cellularA, XN/ACytoplasmicFlagellum FlayesyesN490.115CYT0-0.200913-000iCytoplasmic9.2689.1
FLIY_ECOLIP0AEM9
fliY

yzzR

yzzRb1920 JW1905 Cystine-binding periplasmic protein (CBP) (Protein fliY) (Sulfate starvation-induced protein 7) (SSI7)PeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesN300.555SpI2910.7798-00Yn10-21c29/30oPeriplasmic1096.19
YKGI_ECOLIP75687
ykgI
b0303 JW5039 Uncharacterized protein ykgIPeriplasmicGN/APeriplasmPeriplasmicSECnoyesY230.662SpI2216.2846-00Yn6-17c22/23oUnknown2.5
YAHO_ECOLIP75694
yahO
b0329 JW0321 Uncharacterized protein yahOPeriplasmicGN/APeriplasmPeriplasmicSECnoyesY220.85SpI2113.1005-00Yn8-16c21/22oUnknown2.585.68
YBBV_ECOLIP75711
ybbV
b0510 JW0498 Protein hokBIntegral Inner Membrane BBinout/inIM (predicted)Membrane anchoredSRPSECnoyesN610.11CYT0-0.200913-110o15-33iUnknown2
RZPD_ECOLIP75719
rzpD

ybcT

ybcTb0556 JW5079 Putative Rz endopeptidase from lambdoid prophage DLP12 (EC 3.4.-.-)Required for normal curli expression and biofilm development.PMID: 23378572Integral Inner Membrane B-N/APeriplasmicSRPSECProphage Integrase (EcoGene)nonoN230.233SpI223.631-10Yn6-17c22/23oUnknown228.64
FTSL_ECOLIP0AEN4
ftsL

mraR yabD

mraR yabDb0083 JW0081 Cell division protein ftsLLipoprotein (experimental). Integral Inner Membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membraneLocalizes to potential division sites in filamentous cells. Proven pignal peptidase II substrate. PMID: 1332942PMID: 15174130 PMID: 21890895 PMID: 1332942 PMID: 10027987 PMID: 11703663 PMID: 11948172 PMID: 15165235Inner Membrane LipoproteinEIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SEC, non classicalyesyesN240.167CYT0-0.200913-110o35-57iCytoplasmic8.9625.32
GALP_ECOLIP0AEP1
galP
b2943 JW2910 Galactose-proton symporter (Galactose transporter)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN260.158TMH014.2152-12120i15-44o56-77i84-103o109-130i142-160o172-192i251-274o290-310i322-342o348-373i385-406o418-436iCytoplasmicMembrane10
GLNH_ECOLIP0AEQ3
glnH
b0811 JW0796 Glutamine-binding periplasmic protein (GlnBP)Periplasmic Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350PMID: 18498364 PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSURAyesyesY230.851SpI2219.9168-00Yn7-18c22/23oPeriplasmic1062.21
GLNP_ECOLIP0AEQ6
glnP
b0810 JW0795 Glutamine transport system permease protein glnPIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.119TMH07.24978-350o23-42i54-78o90-113i125-144o190-209iCytoplasmicMembrane10
GLPF_ECOLIP0AER0
glpF
b3927 JW3898 Glycerol uptake facilitator protein (Aquaglyceroporin) Outer membrane. Integral inner membrane (experimental).PMID: 11266632 PMID: 11039922 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN310.125TMH09.6263-660i12-34o46-79i86-108o145-166i178-202o230-251iCytoplasmicMembrane10
YBFN_ECOLIP75734
ybfN

ybfN

ybfNb0682 JW0668 Uncharacterized lipoprotein ybfNOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY190.487SpII1619.5262Pos+2=A00Yn4-14c18/19oUnknown2.577.74
YBFP_ECOLIP75737
ybfP
b0689 JW0676 Uncharacterized lipoprotein ybfPLipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY250.444SpII2215.3591Pos+2=A10Yn7-20c24/25oUnknown2.551.52
GLTJ_ECOLIP0AER3
gltJ
b0654 JW0649 Glutamate/aspartate transport system permease protein gltJIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.108TMH09.38551-550o24-50i71-88o108-127i179-199o211-233iCytoplasmicMembrane10
GLTK_ECOLIP0AER5
gltK
b0653 JW0648 Glutamate/aspartate transport system permease protein gltKIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN310.129TMH07.84867-550o20-44i65-83o95-112i153-178o198-219iCytoplasmicMembrane10
GLTS_ECOLIP0AER8
gltS

gltC

gltCb3653 JW3628 Sodium/glutamate symport carrier protein (Glutamate permease)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN270.145CYT0-0.200913-911Yn7-18c26/27o36-52i72-90o96-119i126-148o160-184i215-239o245-268i275-297o303-323i335-357o369-394iCytoplasmicMembrane10
HDEA_ECOLIP0AES9
hdeA

yhhC yhiB

yhhC yhiBb3510 JW3478 Chaperone-like protein hdeA (10K-S protein)Periplasm Periplasmic Experimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 10623550 PMID: 17085547 PMID: 20080625 PMID: 8455549 PMID: 25403562 PMID: 25403562 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECYfgMyesyesY220.698SpI2116.385-10Yn4-15c21/22oUnknown2.5108.01
HDEB_ECOLIP0AET2
hdeB

yhhD yhiC

yhhD yhiCb3509 JW5669 Protein hdeB (10K-L protein)Periplasm Periplasmic Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 8455550 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 17085547 PMID: 8455550 PMID: 25403562 PMID: 25403562 PMID: 25664544PeriplasmicGN/APeriplasmic (Experimental)SECYfgMyesyesY300.837SpI2916.8932-00Yn10-21c29/30oUnknown2.563.24
YBHM_ECOLIP75769
ybhM
b0787 JW0770 Uncharacterized protein ybhMIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN410.18TMH04.77792-770i21-41o53-70i77-98o104-129i136-155o161-179i209-229oCytoplasmicMembrane1067.12
YCAP_ECOLIP75839
ycaP
b0906 JW0889 UPF0702 transmembrane protein ycaPIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN640.104TMH00.694512-330o18-37i49-69o75-96iCytoplasmic8.9626.62
GFCC_ECOLIP75883
gfcC

ymcB

ymcBb0985 JW0968 Uncharacterized protein gfcC (Group 4 capsule protein C homolog)PeriplasmicGN/APeriplasmicSECyesyesY230.723SpI228.45245-00Yn4-15c22/23oUnknown2.541.93
HDED_ECOLIP0AET5
hdeD

yhiA

yhiAb3511 JW3479 Protein hdeDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.139TMH03.36767-660i23-42o48-69i76-97o103-124i136-155o161-187iCytoplasmicMembrane10
HISJ_ECOLIP0AEU0
hisJ
b2309 JW2306 Histidine-binding periplasmic protein (HBP) Periplasmic Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 19953543 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY230.792SpI2220.7158-10Yn4-14c22/23oPeriplasmic10
HISM_ECOLIP0AEU3
hisM
b2307 JW2304 Histidine transport system permease protein hisMIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.123TMH010.1822-430o20-45i57-79o200-221iCytoplasmicMembrane10
SKP_ECOLIP0AEU7
skp

hlpA ompH

hlpA ompHb0178 JW0173 Chaperone protein skp (DNA-binding 17 kDa protein) (Histone-like protein HLP-1) (Seventeen kilodalton protein)Periplasm Periplasm Periplasm Periplasmic Periplasmic Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamA. PMID: 2167239 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350PMID: 21370981 PMID: 19525348 PMID: 23641708 PMID: 2318304 PMID: 1838129 PMID: 8730870 PMID: 2167239 PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasmPeriplasmic(experimental)SECyesyesY210.881SpI2022.0906-00Yn4-15c20/21oPeriplasmic1048.54
HYFE_ECOLIP0AEW1
hyfE
b2485 JW2470 Hydrogenase-4 component E (EC 1.-.-.-)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN540.165TMH04.55769-770o6-24i31-54o60-80i92-114o126-143i152-171o177-196iCytoplasmicMembrane10
GFCA_ECOLIP75885
gfcA

ymcD

ymcDb0987 JW5133 Threonine-rich inner membrane protein gfcA (Group 4 capsule protein A homolog)Integral inner membrane (experimental).When the protein is over-expressed protein is located on the Inner Membrane.PMID: 15919996Integral Inner Membrane Bout/outIM (predicted)Cell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesY220.858SpI2131.9353-20Yn5-16c21/22oCytoplasmicMembrane10113.93
YCDZ_ECOLIP75916
ycdZ
b1036 JW5147 Inner membrane protein ycdZIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN650.232TMH014.4904-550o20-42i49-70o76-94i99-117o123-147iCytoplasmicMembrane10
YMDA_ECOLIP75917
ymdA
b1044 JW1031 Uncharacterized protein YmdAPeriplasmicGN/APeriplasmicSECnoyesY230.463SpI2213.2508-10Yn11-22c28/29oExtracellular9.6536.55
KGTP_ECOLIP0AEX3
kgtP

witA

witAb2587 JW2571 Alpha-ketoglutarate permeaseIntegral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECnoyesN90.105TMH02.8422-12120i21-42o62-84i96-116o122-139i160-183o195-214i244-266o278-299i311-331o337-360i372-393o405-423iCytoplasmicMembrane10
LIVH_ECOLIP0AEX7
livH
b3457 JW3422 High-affinity branched-chain amino acid transport system permease protein livH (LIV-I protein H)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN450.186TMH016.6878-880o20-39i46-64o70-89i105-122o158-175i204-230o242-270i282-301oCytoplasmicMembrane10
MALE_ECOLIP0AEX9
malE
b4034 JW3994 Maltose-binding periplasmic protein (MMBP) (Maltodextrin-binding protein) Periplasmic Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. MalE is maintained in an unfolded conformation by the cytoplasmic chaperone SecB. Potential Skp substrate PMID: 19953543 PMID: 9298646 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 18498364 PMID: 1989077 PMID: 19953543 PMID: 9298646 PMID: 3009404 PMID: 25403562 PMID: 25403562PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SecBSECSURA,SKP,YfgMyesyesY270.712SpI2617.3972-00Yn9-17c26/27oPeriplasmic1096.4
MARC_ECOLIP0AEY1
marC

ydeB ydeC

ydeB ydeCb1529 JW1522 UPF0056 inner membrane protein marCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN380.16TMH010.7793-550o12-32i44-62o68-89i151-172o192-214iCytoplasmicMembrane105
FLGA_ECOLIP75933
flgA
b1072 JW1059 Flagella basal body P-ring formation protein flgAPeriplasmicPeriplasmic chaperone for P ring protein. PMID: 12730325PMID: 12631703 PMID: 12730325PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SEC FlayesyesY220.722SpI215.00523-00Yn8-16c21/22oPeriplasmic9.7612.09
YCFL_ECOLIP75946
ycfL
b1104 JW1090 Uncharacterized protein ycfLOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesY240.214SpII1819.4253Pos+2=R00Yn5-16c21/22oUnknown270.14
YMFA_ECOLIP75962
ymfA
b1122 JW5164 Inner membrane protein ymfAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN290.212TMH06.47267-220i7-28o117-139iCytoplasmicMembrane10
MDFA_ECOLIP0AEY8
mdfA

cmlA cmr

cmlA cmrb0842 JW0826 Multidrug translocase mdfA (Chloramphenicol resistance pump cmr)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN320.122TMH06.20433-12120i15-33o53-72i84-103o109-130i142-164o170-188i221-246o258-275i287-308o314-335i347-367o379-399iCytoplasmicMembrane10
MLTD_ECOLIP0AEZ7
mltD

dniR yafG

dniR yafGb0211 JW5018 Membrane-bound lytic murein transglycosylase D (EC 4.2.2.n1) (Murein hydrolase D) (Regulatory protein dniR)Lipoprotein (experimental). Outer membrane LipoproteinProven signal peptidase II substrate. PMID: 15174133PMID: 15174130 PMID: 15174133Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN270.22SpII1518.5563Pos+2=Q00Yn5-13c18/19oUnknown229.22
MODB_ECOLIP0AF01
modB

chlJ

chlJb0764 JW0747 Uncharacterized protein yjhHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN340.137TMH08.18777-550o20-37i49-70o90-115i136-158o198-220iCytoplasmicMembrane1020.17
MOTB_ECOLIP0AF06
motB
b1889 JW1878 Motility protein B (Chemotaxis protein MotB)Cell inner membrane Membrane anchored Integral inner membrane MotB has one membrane-spanning alpha-helix but the majority of the molecule is in the periplasmic space with a peptidoglycan-binding domain near its C-terminus PMID: 2447650PMID: 19364805 PMID: 23193105 PMID: 2447650 PMID: 12730325 PMID: 2447650Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SRPSECyesyesN320.155CYT0-0.200913-110o28-47iCytoplasmicMembrane7.8833.3
YMFE_ECOLIP75968
ymfE
b1138 JW5166 Uncharacterized protein ymfEIntegral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN370.102TMH01.7441-440i21-39o59-79i151-173o185-208iCytoplasmicMembrane107.7
Y1142_ECOLIP75971
b1142
b1142 JW1128 Putative uncharacterized protein b1142Integral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECpseudogene (Uniprot), pseudogene (Ecogene), pseudogene (Ochman & Davalos, 2006)nonoN630.119CYT0-0.200913-220i41-62o74-92iUnknown254.37
YMFR_ECOLIP75979
ymfR
b1150 JW1136 Uncharacterized protein ymfRIntegral Inner Membrane BBinoutin/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN220.153TMH011.0494-21Yn4-17c21/22o31-49iCytoplasmicMembrane9.8216.11
YCGJ_ECOLIP76001
ycgJ
b1177 JW1166 Uncharacterized protein ycgJ Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesY230.404SpI228.58297-00Yn6-16c24/25oUnknown2.577.92
MURJ_ECOLIP0AF16
murJ

mviN yceN

mviN yceNb1069 JW1056 Virulence factor mviN homologCell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN220.165SpI230.241812-13130o27-45i82-110o130-154i161-182o188-205i236-262o274-292i313-334o354-371i383-402o408-429i441-466o478-501iCytoplasmicMembrane10
NARV_ECOLIP0AF32
narV
b1465 JW1460 Respiratory nitrate reductase 2 gamma chain (EC 1.7.99.4)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN190.114TMH010.9916-550o12-29i50-69o89-111i132-151o180-199iCytoplasmicMembrane10
YJBB_ECOLIP0AF43
yjbB
b4020 JW3980 Uncharacterized protein yjbBIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN240.13TMH01.33234-88Yn2-13c17/18o45-64i76-98o104-121i133-151o171-194i206-226o238-262i274-294oCytoplasmicMembrane104.03
YJCD_ECOLIP0AF52
yjcD
b4064 JW4025 Putative permease yjcDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN590.114CYT0-0.200913-13130i29-49o55-74i81-101o107-129i141-165o171-195i202-220o256-280i301-321o333-350i357-378o390-416i428-448oCytoplasmicMembrane10
YJEI_ECOLIP0AF70
yjeI
b4144 JW5736 Uncharacterized protein yjeIOuter membrane Lipoprotein (experimental) Outer membrane protein (experimental). Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130PMID: 15174130 PMID: 12488465 PMID: 20932056Outer Membrane LipoproteinISoluble IM FractionOuter membrane Lipoprotein (Experimental)SECLOLyesyesY280.443SpII1820.5638Pos+2=S00Yn6-17c22/23oUnknown2.587.52
PLIG_ECOLIP76002
pliG

ycgK

ycgKb1178 JW1167 Uncharacterized protein ycgKPeriplasm SecB substrate. Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 16352602 PMID: 20734102 PMID: 25403562 PMID: 25403562PeriplasmicGN/APeriplasmicSECBSECnoyesY230.798SpI2219.6754-00Yn9-17c22/23oUnknown2.572.87
YMGE_ECOLIP76011
ymgE

tag

tagb1195 JW1184 UPF0410 protein ymge (Transglycosylase-associated gene protein)Integral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN270.106TMH011.3901-330o6-21i28-47o59-81iCytoplasmicMembrane1016.22
YDBL_ECOLIP76076
ydbL
b1383 JW5216 Uncharacterized protein ydbLPeriplasmicGN/APeriplasmicSECyesyesY220.838SpI2115.5115-10Yn4-15c21/22oUnknown2.551.39
LPTF_ECOLIP0AF98
lptF

yjgP

yjgPb4261 JW4218 Lipopolysaccharide export system permease protein lptFIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN380.124CYT0-0.200913-660i16-39o59-76i97-119o268-289i296-316o328-348iCytoplasmicMembrane1013.66
NARX_ECOLIP0AFA2
narX

narR

narRb1222 JW1213 Nitrate/nitrite sensor protein narX (EC 2.7.13.3)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN470.224SpI334.68578-220i12-36o154-174iCytoplasmicMembrane1044.11
NFRB_ECOLIP0AFA5
nfrB
b0569 JW0558 Bacteriophage N4 adsorption protein BCell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN200.134CYT0-0.200913-440o12-42i365-384o390-410i422-443oCytoplasmicMembrane107.03
NHAB_ECOLIP0AFA7
nhaB
b1186 JW1175 Na(+)/H(+) antiporter nhaB (Sodium/proton antiporter nhaB)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN440.149TMH010.0118-12120o20-47i59-77o89-108i120-141o147-165i202-219o239-260i299-331o351-368i389-413o453-471i478-498oCytoplasmicMembrane10
YNBA_ECOLIP76090
ynbA
b1408 JW1405 Inner membrane protein ynbAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN500.126TMH0-0.193337-550o31-64i85-107o113-132i152-168o174-191iCytoplasmicMembrane1011.98
YDCZ_ECOLIP76111
ydcZ
b1447 JW1442 Inner membrane protein ydcZIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN610.165TMH08.79151-54Yn4-19c24/25o34-55i67-88o94-114i126-145oCytoplasmicMembrane1014.36
YNCH_ECOLIP76118
yncH
b1455 JW5235 Uncharacterized protein yncHPeriplasmicGIM (predicted)CytoplasmicSECnoyesN400.113CYT0-0.200913-00Yn2-13c18/19oUnknown235.64
NIKC_ECOLIP0AFA9
nikC
b3478 JW3443 Nickel transport system permease protein nikCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN250.156TMH010.244-660i12-32o75-100i121-148o177-196i203-222o242-260iCytoplasmicMembrane10
NLPI_ECOLIP0AFB1
nlpI

yhbM

yhbMb3163 JW3132 Lipoprotein nlpIOuter membrane Lipoprotein (experimental) Proven pignal peptidase II substrate. PMID: 15174130 Induction of NlpI inhibits nucleoid division and halts cell growth. Elevation of NlpI expression enhances the transcription and the outer membrane localization of the heat shock protein IbpA and IbpB. NlpI physically interacts with IbpB. The C-terminus of NlpI is critical for the enhancement in IbpA and IbpB production, and the N-terminus of NlpI is required for the outer membrane localization of NlpI, IbpA, and IbpB. PMID: 25699035PMID: 15174130 PMID: 25699035Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN260.209SpII1814.4676Pos+2=S00Yn8-19c31/32oCytoplasmicMembrane1056.21
NUOA_ECOLIP0AFC3
nuoA
b2288 JW2283 NADH-quinone oxidoreductase subunit A (EC 1.6.99.5) (NADH dehydrogenase I subunit A) (NDH-1 subunit A) (NUO1)Cell inner membrane Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 15919996 PMID: 21541338Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN440.182TMH012.5237-330o12-37i68-88o100-122iCytoplasmicMembrane1048.69
NUOH_ECOLIP0AFD4
nuoH
b2282 JW2277 NADH-quinone oxidoreductase subunit H (EC 1.6.99.5) (NADH dehydrogenase I subunit H) (NDH-1 subunit H) (NUO8)Integral inner membrane (experimental).PMID: 20732298 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN330.139TMH07.32865-890i12-32o52-69i81-100o112-133i154-174o186-207i234-257o269-289i301-322oCytoplasmicMembrane10
NUOJ_ECOLIP0AFE0
nuoJ
b2280 JW2275 NADH-quinone oxidoreductase subunit J (EC 1.6.99.5) (NADH dehydrogenase I subunit J) (NDH-1 subunit J) (NUO10)Integral inner membrane (experimental).PMID: 20732298 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.504TMH010.2272-54Yn3-14c18/19o28-49i56-74o94-112i133-158oCytoplasmicMembrane1082.78
YNEN_ECOLIP76136
safA

yneN

yneNb1500 JW1494.1 Two-component-system connector protein yneN (Regulatory protein b1500)Cell inner membranePMID: 17998538Integral Inner Membrane BBinout/inIM (predicted)Cell inner membrane | Single-pass type II membrane protein-SRPSECnoyesN400.109CYT0-0.200913-110o19-40iCytoplasmicMembrane9.82
YNEE_ECOLIP76146
yneE
b1520 JW5245 UPF0187 protein yneEIntegral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN380.113TMH013.5444-340i28-46o52-70i208-229o235-255iCytoplasmicMembrane9.82
RZPQ_ECOLIP76158
rzpQ

ydfP

ydfPb1553 JW1545 Uncharacterized protein rzpQPeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoN230.245SpI220.491707-10Yn7-17c22/23oUnknown219.08
NUOK_ECOLIP0AFE4
nuoK
b2279 JW2274 NADH-quinone oxidoreductase subunit K (EC 1.6.99.5) (NADH dehydrogenase I subunit K) (NDH-1 subunit K) (NUO11)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 20732298 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN460.166TMH08.16488-330o6-24i29-54o60-81iCytoplasmicMembrane10
NUOM_ECOLIP0AFE8
nuoM
b2277 JW2272 NADH-quinone oxidoreductase subunit M (EC 1.6.99.5) (NADH dehydrogenase I subunit M) (NDH-1 subunit M) (NUO13)Integral inner membrane (experimental).PMID: 20732298 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN590.116TMH06.38779-14140o6-23i30-48o77-103i115-134o140-159i180-199o219-244i256-274o286-307i314-333o345-363i383-405o417-440i461-481oCytoplasmicMembrane10
NUON_ECOLIP0AFF0
nuoN
b2276 JW2271 NADH-quinone oxidoreductase subunit N (EC 1.6.99.5) (NADH dehydrogenase I subunit N) (NDH-1 subunit N) (NUO14)Integral inner membrane (experimental).PMID: 20732298 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN610.262TMH09.81822-14140o6-28i35-55o75-93i105-122o128-147i159-181o201-226i238-259o271-290i297-316o328-351i371-394o406-426i447-470oCytoplasmicMembrane10
NUPC_ECOLIP0AFF2
nupC

cru

crub2393 JW2389 Nucleoside permease nupC (Nucleoside-transport system protein nupC)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN270.126TMH00.463943-890o6-23i30-48o87-108i167-187o193-212i242-271o277-298i341-361o381-399iCytoplasmicMembrane10
NUPG_ECOLIP0AFF4
nupG
b2964 JW2932 Nucleoside permease NupGIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.127TMH010.0497-12120i12-31o37-58i70-89o95-116i137-155o161-179i211-235o255-275i282-300o306-327i348-369o381-402iCytoplasmicMembrane10
YNFD_ECOLIP76172
ynfD
b1586 JW5259 Uncharacterized protein ynfDPeriplasmicExperimentally verified gene-product. PMID: 9868784PMID: 9868784PeriplasmicGSoluble IM FractionPeriplasmic (Experimental)SECnoyesY220.874SpI2122.7125-00Yn3-14c21/22oExtracellular9.6579.09
YDGK_ECOLIP76180
ydgK
b1626 JW1618 Inner membrane protein ydgKIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN400.147TMH019.3259-440i12-36o56-78i90-108o114-133iCytoplasmicMembrane10
YDHO_ECOLIP76190
ydhO
b1655 JW5270 Uncharacterized protein ydhOPeriplasmicGN/APeriplasmicSECyesyesY280.421SpI2715.3587-00Yn8-19c27/28oUnknown6.49
OPPB_ECOLIP0AFH2
oppB
b1244 JW1236 Oligopeptide transport system permease protein oppBIntegral inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm. Opp-LacZ hybrid proteins were used to show that OppB, OppC and OppD are membrane-associated proteins. PMID: 2821267PMID: 15919996 PMID: 2821267Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN480.124TMH03.13181-660i12-30o100-121i133-161o173-192i228-254o274-300iCytoplasmicMembrane10
OPPC_ECOLIP0AFH6
oppC
b1245 JW1237 Oligopeptide transport system permease protein oppCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN640.213TMH00.755301-660i40-64o103-128i140-158o164-183i218-247o267-290iCytoplasmicMembrane10
OSMY_ECOLIP0AFH8
osmY
b4376 JW4338 Osmotically-inducible protein YPeriplasmic Periplasmic Extra-celullar Peripheral inner membrane protein facing the periplasm (potential). The most efficient excreting fusion partner, OsmY, was used as a carrier protein to excrete heterologous proteins into the medium. PMID: 18727129 The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane PMID: 12175925PMID: 1317380 PMID: 9298646 PMID: 18727129 PMID: 12878000Periplasmic, Extra-cellularG, XN/APeriplasmPeriplasmic (Experimental)SECyesyesY290.787SpI2823.4143-00Yn12-23c28/29oPeriplasmic10108.86
PBP7_ECOLIP0AFI5
pbpG

yohB

yohBb2134 JW5355 D-alanyl-D-alanine endopeptidase (DD-endopeptidase) (EC 3.4.21.-) (Penicillin-binding protein 7) (PBP-7)PeriplasmicPMID: 7721700PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY260.58SpI2517.1021-00Yn6-17c25/26oPeriplasmic1082.72
YDIY_ECOLIP76206
ydiY
b1722 JW1711 Uncharacterized protein ydiYPeriplasmicGN/APeriplasmicSECyesyesY260.481SpI2520.5374-00Yn7-18c23/24oUnknown2.515.66
YNIB_ECOLIP76208
yniB
b1726 JW1715 Uncharacterized protein yniBIntegral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN440.107TMH05.0431-340o15-33i45-63o83-101i150-172oCytoplasmicMembrane1040.74
YDJY_ECOLIP76220
ydjY
b1751 JW5283 Uncharacterized protein ydjY 4Fe-4S ferredoxin-type, iron-sulphur binding domain (IPR017896) Inner Membrane LipoproteinESoluble IM FractionInner membrane lipoproteinSECLOLnoyesN340.346SpII2615.9526Pos+2=D00Yn13-24c29/30oUnknown2.596.26
YNJA_ECOLIP76222
ynjA
b1753 JW1742 Uncharacterized protein ynjAIntegral Inner Membrane BBinout/inIM (predicted)CytoplasmicSRPSECyesyesN260.102CYT0-0.200913-010o33-51iCytoplasmicMembrane9.8241.21
PERM_ECOLIP0AFI9
perM

yfgO

yfgOb2493 JW2478 Putative permease perMIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.135TMH020.7793-770o20-51i72-93o151-177i217-236o242-270i282-301o307-332iCytoplasmicMembrane1026
PITA_ECOLIP0AFJ7
pitA

pit

pitb3493 JW3460 Low-affinity inorganic phosphate transporter 1Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN390.147TMH08.59981-1090o12-32i52-76o96-116i123-143o155-177i208-227o233-255i379-402o474-494iCytoplasmicMembrane10
PNUC_ECOLIP0AFK2
pnuC
b0751 JW0734 Nicotinamide riboside transporter pnuCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.115TMH03.57186-670o22-40i47-65o71-88i109-139o159-178i185-205o211-226iCytoplasmicMembrane10
POTB_ECOLIP0AFK4
potB
b1125 JW1111 Spermidine/putrescine transport system permease protein potBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.107TMH05.71787-670o6-26i54-78o90-108i120-138o144-163i184-214o242-262iCytoplasmicMembrane10
POTC_ECOLIP0AFK6
potC
b1124 JW1110 Spermidine/putrescine transport system permease protein potCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN600.118TMH010.8409-660i12-30o64-86i98-122o128-151i172-195o230-252iCytoplasmicMembrane10
YNJF_ECOLIP76226
ynjF
b1758 JW1747 Inner membrane protein ynjFIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN430.114CYT0-0.200913-550o33-60i81-102o114-135i147-167o173-195iCytoplasmicMembrane10
YNJH_ECOLIP76227
ynjH
b1760 JW1749 Uncharacterized protein ynjH Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECnoyesY190.569SpI1811.8884-00Yn4-14c18/19oCytoplasmicMembrane9.8627.32
YOAI_ECOLIP76239
yoaI
b1788 JW5885 Xanthosine permease (Xanthosine transporter)Integral Inner Membrane BBinoutout/inIM (predicted)Membrane | Single-pass membrane protein-SRPSECnoyesN210.164CYT0-0.200913-110o6-30iCytoplasmicMembrane9.82
LEUE_ECOLIP76249
leuE

yeaS

yeaSb1798 JW1787 Leucine efflux proteinIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN470.141TMH012.792-660o12-32i44-69o81-99i120-142o154-179i191-209oCytoplasmicMembrane10
POTD_ECOLIP0AFK9
potD
b1123 JW1109 Spermidine/putrescine-binding periplasmic protein (SPBP)Periplasmic Periplasmic Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 1939142 PMID: 9298646 PMID: 22030350PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY240.865SpI2325.0348-00Yn8-18c23/24oPeriplasmic10
POTI_ECOLIP0AFL1
potI
b0857 JW0841 Putrescine transport system permease protein potIIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN260.124TMH014.5424-670i12-35o60-90i102-129o141-163i184-206o212-232i244-264oCytoplasmicMembrane10
PPIA_ECOLIP0AFL3
ppiA

rot rotA

rot rotAb3363 JW3326 Peptidyl-prolyl cis-trans isomerase A (PPIase A) (EC 5.2.1.8) (Cyclophilin A) (Rotamase A)Periplasmic Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 2190212 PMID: 22030350PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY250.753SpI2424.1414-00Yn4-15c24/25oPeriplasmic1073.9
PQIA_ECOLIP0AFL9
pqiA

pqi5A

pqi5Ab0950 JW0933 Paraquat-inducible protein AIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN330.119CYT0-0.200913-880i53-76o96-122i142-160o166-185i257-277o304-329i350-371o377-400iCytoplasmicMembrane10
PROX_ECOLIP0AFM2
proX

proU

proUb2679 JW2654 Glycine betaine-binding periplasmic protein Periplasmic Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Identified DsbA substrate PMID: 19953543 PMID: 3305496 Disulfide bridge (157 163) Disulfide bond-containing protein PMID: 25664544PMID: 18498364 PMID: 19953543 PMID: 3305496 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSURA,DSBAyesyesY220.863SpI2116.9579-00Yn5-16c21/22oPeriplasmic1052.14
YECT_ECOLIP76296
yecT
b1877 JW5310 Uncharacterized protein yecTPeriplasmicGN/APeriplasmicSECnoyesY190.831SpI1811.1311-00Yn4-14c18/19oUnknown4.931.26
FLHE_ECOLIP76297
flhE
b1878 JW1867 Flagellar protein flhEPart of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECFlayesyesY170.511SpI168.24229-00Yn3-14c26/27oUnknown2.568.14
YECR_ECOLIP76308
yecR
b1904 JW1892 Uncharacterized protein yecRLipoprotein (experimental) Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/AOuter membrane Lipoprotein (Experimental)SECLOLnoyesN250.325SpII158.98675Pos+2=T00Yn3-14c22/23oUnknown215.11
YADH_ECOLIP0AFN6
yadH
b0128 JW0124 Inner membrane transport permease yadHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.101TMH08.75539-67Yn4-9c14/15o24-46i58-84o104-132i139-164o170-191i203-221o227-248iCytoplasmicMembrane104.72
YBHR_ECOLIP0AFP9
ybhR
b0792 JW5803 Inner membrane transport permease ybhRIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.153CYT0-0.200913-660i21-41o173-197i218-243o255-277i284-302o345-363iCytoplasmicMembrane10
YBHS_ECOLIP0AFQ2
ybhS
b0793 JW0777 Inner membrane transport permease ybhSIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN670.102CYT0-0.200913-660i29-47o182-205i232-255o261-283i295-314o346-370iCytoplasmicMembrane107.64
YCHM_ECOLIP0AFR2
ychM

ychM

ychMb1206 JW5189 Putative sulfate transporter ychMCell inner membrane Integral inner membrane (experimental).PMID: 23278959 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN690.11CYT0-0.200913-1190o33-53i60-80o100-118i130-152o172-198i210-231o278-300i358-388o408-438iCytoplasmicMembrane10
YCJO_ECOLIP0AFR7
ycjO
b1311 JW1304 Inner membrane ABC transporter permease protein ycjOIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.147TMH012.2234-65Yn14-25c30/31o77-98i110-130o163-189i210-233o265-283iCytoplasmicMembrane10
YEDR_ECOLIP76334
yedR
b1963 JW1946 Inner membrane protein yedRIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN230.118TMH00.40092-230o6-22i43-62o86-109iCytoplasmicMembrane1043.19
YOEA_ECOLIP76356
yoeA
b4582 JW1980 b1995 b1998Putative uncharacterized protein yoeAOuter Membrane b-barrel proteinHN/ACell inner membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinSECBAMpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoY290.402SpI288.15876-00Yn12-23c28/29oOuterMembrane9.93
YEGJ_ECOLIP76394
yegJ
b2071 JW2056 Uncharacterized protein YegJPeriplasmicGN/APeriplasmicSECnoyesY190.264SpI199.75379-00Yn4-14c19/20oUnknown2.579.39
YDCV_ECOLIP0AFR9
ydcV
b1443 JW1438 Inner membrane ABC transporter permease protein ydcVIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN330.134TMH08.81303-660i12-33o71-91i103-127o133-153i189-212o232-256iCytoplasmicMembrane10
LSRD_ECOLIP0AFS1
lsrD

ydeZ

ydeZb1515 JW1508 Uncharacterized protein yjhG (EC 4.-.-.-)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN240.162TMH09.62635-99Yn9-17c23/24o33-56i63-82o88-110i117-140o160-181i212-234o240-260i267-286o292-310iCytoplasmicMembrane10
TQSA_ECOLIP0AFS5
tqsA

ydgG

ydgGb1601 JW1593 AI-2 transport protein tqsA (Transport of quorum-sensing signal protein)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.372TMH08.36419-880i12-31o37-55i62-83o144-165i197-218o224-246i258-279o299-325iCytoplasmicMembrane10
YDIK_ECOLIP0AFS7
ydiK
b1688 JW1678 UPF0118 inner membrane protein ydiKIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.172TMH013.7653-990i12-31o37-53i65-86o160-179i191-208o214-235i247-271o277-295i307-334oCytoplasmicMembrane10
YEBA_ECOLIP0AFS9
yebA
b1856 JW5304 Uncharacterized metalloprotease yebA (EC 3.4.24.-) (ORFU)Integral Inner Membrane BBoutin/outIM (predicted)Cell membrane | Single-pass membrane proteinCytoplasmicSRPSECyesyesN120.112CYT0-0.200913-110i21-39oUnknown233.61
YEGR_ECOLIP76406
yegR
b2085 JW5837 Uncharacterized protein yegROuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLyesyesY220.281SpII1715.1742Pos+2=A00Yn4-14c22/23oUnknown2.554.53
YFAT_ECOLIP76466
yfaT
b2229 JW2223 Uncharacterized protein yfaTPeriplasmicGN/APeriplasmicSECnoyesY200.277SpI193.99575-00Yn4-15c19/20oCytoplasmicMembrane9.8226.12
YFCO_ECOLIP76498
yfcO
b2332 JW2329 Uncharacterized protein yfcOPutative chaperone–usher fimbrial operon yfc (class π); Unkown functionPMID: 20345943PeriplasmicGSoluble IM FractionPeriplasmicfimbriaeSECnoyesY220.835SpI2114.5584-00Yn6-16c21/22oExtracellular9.6541.82
YFCQ_ECOLIP76500
yfcQ
b2334 JW2331 Uncharacterized fimbrial-like protein yfcQPutative chaperone–usher fimbrial operon yfc (class π); Fimbrial Subunit.PMID: 20345943PeriplasmicGN/APeriplasmicfimbriaeSECyesyesY190.841SpI1813.6429-00Yn4-11c18/19oUnknown2.596.19
YECS_ECOLIP0AFT2
yecS
b1918 JW1903 Inner membrane amino-acid ABC transporter permease protein yecSIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN700.103TMH08.62641-330o21-45i57-78o186-206iCytoplasmicMembrane104.96
YEJB_ECOLIP0AFU0
yejB
b2178 JW2166 Inner membrane ABC transporter permease protein yejBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN190.157TMH04.74055-660i9-30o135-156i168-194o224-247i282-309o329-355iCytoplasmicMembrane10
YFBS_ECOLIP0AFU2
yfbS
b2292 JW2289 Uncharacterized transporter yfbSIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN210.196TMH07.38351-10100i5-22o28-45i57-83o95-124i136-155o175-195i470-493o513-538i550-568o588-608iCytoplasmicMembrane10
YIBH_ECOLIP0AFV0
yibH
b3597 JW3571 Inner membrane protein yibHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinMembrane anchoredSRPSECyesyesN530.113TMH02.25888-21Yn3-13c18/19o28-49iCytoplasmicMembrane1023.7
SPR_ECOLIP0AFV4
spr

yeiV

yeiVb2175 JW2163 Lipoprotein sprOuter membrane Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY340.288SpII2611.4182Pos+2=S00Yn14-24c29/30oUnknown2.536.96
YFCR_ECOLIP76501
yfcR
b2335 JW2332 Uncharacterized protein yfcRPutative chaperone–usher fimbrial operon yfc (class π); Fimbrial Subunit.PMID: 20345943PeriplasmicGN/APeriplasmicfimbriaeSECyesyesY290.68SpI2811.4125-00Yn10-22c30/31oUnknown2.549.6
YFDX_ECOLIP76520
yfdX
b2375 JW2372 Protein yfdXPeriplasmicPMID: 11157960PeriplasmicGN/APeriplasmic (Experimental)SECyesyesY220.881SpI2120.5809-00Yn4-15c21/22oUnknown2.549.64
YFDY_ECOLIP76521
yfdY
b2377 JW2374 Uncharacterized protein yfdYIntegral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN280.123TMH019.5928-330o6-25i32-49o55-75iCytoplasmicMembrane1058.61
RSEA_ECOLIP0AFX7
rseA

mclA yfiJ

mclA yfiJb2572 JW2556 Sigma-E factor negative regulatory proteinIntegral inner membrane protein. Integral inner membrane protein. Cell inner membrane Integral inner membrane. Nucleoid (experimental). Responce to stress complex RpoE-RseA-RseB. Overproduction of N-terminal domain of RseA alone was shown to inhibit σE activity. RseC which is an inner membrane protein, positively modulates σE activity. The periplasmic domain of RseA interacts with RseB while its cytoplasmic domain interacts with RpoE, thereby sequestering RpoE away from the transcription complex. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428 The N-terminal domain of RseA sequesters σE in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. PMID: 11777003 Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase. PMID: 12718891PMID: 9159523 PMID: 9159522 PMID: 12183369 PMID: 17360428 PMID: 11777003 PMID: 12718891Integral Inner Membrane B-IM (predicted-detected)Cell membrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN620.226CYT0-0.200913-000iUnknown259.17
RSEB_ECOLIP0AFX9
rseB
b2571 JW2555 Sigma-E factor regulatory protein rseBPeriplasm. Periplasm. Periplasm.Responce to stress complex RpoE-RseA-RseB. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428 The N-terminal domain of RseA sequesters σE in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. PMID: 11777003 PMID: 11777003PMID: 9159523 PMID: 9159522 PMID: 17360428 PMID: 11777003PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY240.61SpI2310.5992-00Yn4-16c28/29oPeriplasmic9.7615.17
SANA_ECOLIP0AFY2
sanA

yeiF

yeiFb2144 JW2132 Protein sanASingle pass inner membrane protein.Related to vancomycin resistance and when overexpressed, is able to complement the permeability defects of a vancomycin-susceptible mutant.PMID: 8550448Integral Inner Membrane BBoutin/outN/ACell inner membrane | Single-pass membrane proteinPeriplasmicSRPSECyesyesN230.14TMH06.95693-10Yn5-18c22/23oCytoplasmicMembrane1010.99
SBMA_ECOLIP0AFY6
sbmA
b0377 JW0368 Protein sbmAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN300.182TMH018.7145-880i12-30o58-78i85-112o140-164i206-227o239-263i309-326o332-353iCytoplasmicMembrane10
YFEZ_ECOLIP76538
yfeZ
b2433 JW2426 Inner membrane protein yfeZIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.118TMH01.95358-440i21-41o61-78i90-110o116-135iCytoplasmicMembrane1040.67
YFFQ_ECOLIP76548
yffQ
b2448 Uncharacterized protein yffQPeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoN190.652SpI183.21713-00Yn3-13c18/19oUnknown4.9
YFGI_ECOLIP76573
yfgI
b2506 JW2490 Uncharacterized protein yfgIPeriplasmicGN/APeriplasmicSECnoyesY200.625SpI197.20342-00Yn4-14c32/33oUnknown2.511.76
TRKH_ECOLIP0AFZ7
trkH
b3849 JW5576 Trk system potassium uptake protein trkHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006)yesyesN410.127TMH06.71344-10110i12-32o38-57i69-90o136-163i184-204o241-262i274-294o331-363i396-417o423-444i456-481oCytoplasmicMembrane10
WZZE_ECOLIP0AG00
wzzE

wzz yifC

wzz yifCb3785 JW5601 Lipopolysaccharide biosynthesis protein wzzEIntegral inner membrane (experimental). Integral Membrane Protein Integral Membrane ProteinPMID: 15919996 PMID: 16816184 PMID: 16199561Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN470.253TMH08.70367-220o31-50i323-342oCytoplasmicMembrane1019.93
SOHB_ECOLIP0AG14
sohB
b1272 JW1264 Probable protease sohB (EC 3.4.21.-)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionCell inner membrane | Single-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.141CYT0-0.200913-210o12-32iCytoplasmicMembrane108.79
YIBN_ECOLIP0AG27
yibN
b3611 JW3586 Uncharacterized protein yibNInner membrane (experimental).PMID: 15919657Integral Inner Membrane BBinout/inIM (predicted), Detected in IM FractionMembrane anchoredSRPSECyesyesN360.109CYT0-0.200913-110o12-30iUnknown248.32
RHTB_ECOLIP0AG34
rhtB

yigK

yigKb3824 JW5585 Homoserine/homoserine lactone efflux proteinIntegral Membrane ProteinPMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECpseudogene (Ochman & Davalos, 2006)noyesN250.247TMH011.1132-54Yn7-18c23/24o39-65i116-138o144-168i188-205oCytoplasmicMembrane10
YGAZ_ECOLIP76630
ygaZ
b2682 JW2657 Inner membrane protein ygaZIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN360.194TMH04.25735-560o22-39i51-70o76-94i140-163o175-193i205-238oCytoplasmicMembrane10
YQIJ_ECOLIP76657
yqiJ
b3050 JW3022 Inner membrane protein yqiJIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN390.125TMH03.14983-330i12-35o66-86i98-117oCytoplasmicMembrane1012.35
YZGL_ECOLIP76692
yzgL
b3427 JW3390 Putative uncharacterized protein yzgLIntegral Inner Membrane BBpolyin/inIM (predicted)Integral Membrane ProteinSRPSECpseudogene (Uniprot)nonoN230.127TMH017.4708-220i12-40o46-69iCytoplasmicMembrane1029.62
RHTC_ECOLIP0AG38
rhtC

yigJ

yigJb3823 JW5586 Threonine efflux proteinIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006)yesyesN250.144TMH08.74493-660o6-26i38-63o69-87i119-140o152-174i186-204oCytoplasmicMembrane10
RMUC_ECOLIP0AG71
rmuC

yigN

yigNb3832 JW3809 DNA recombination protein rmuCSingle-pass integral inner membrane proteinBased on the fact that RmuC is a DNA recombination enzyme we conluded that its N-terminus lays in the periplasm.PMID: 16011798Integral Inner Membrane BBinout/inN/ACytoplasmicSRPSECyesyesN290.536SpI281.08238-110o6-25iUnknown215.99
SUBI_ECOLIP0AG78
sbp
b3917 JW3888 Sulfate-binding protein (Sulfate starvation-induced protein 2) (SSI2)PeriplasmicPotential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9298646PMID: 18498364 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSURAyesyesY200.802SpI1915.2757-00Yn4-14c19/20oPeriplasmic1070.13
UGPB_ECOLIP0AG80
ugpB
b3453 JW3418 sn-glycerol-3-phosphate-binding periplasmic protein ugpB Periplasmic Identified DsbA substrate PMID: 19953543 PMID: 3062310 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 3062310 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.804SpI2317.1867-00Yn7-18c23/24oPeriplasmic1064.05
PSTS_ECOLIP0AG82
pstS

phoS

phoSb3728 JW3706 Phosphate-binding protein pstS (PBP)PeriplasmicPotential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Copurifies with trigger factor in pull down assays. PMID: 9298646PMID: 18498364 PMID: 19737520 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TFSECSURAyesyesY260.546SpI2523.9055-10Yn6-17c25/26oPeriplasmic1079.81
HOKE_ECOLIP77091
hokE

ybdY

ybdYb4415 JW5084 Protein hokEPotentially toxic protein.PMID: 19121005Integral Inner Membrane BBinoutin/outN/AMembrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN200.132SpI242.84692-10Yn6-24c29/30oPeriplasmic9.9651.96
YKFB_ECOLIP77162
ykfB
b0250 JW0239 Uncharacterized protein ykfB Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoY240.716SpI2317.8556-00Yn11-19c23/24oUnknown2.559.81
YKGH_ECOLIP77180
ykgH
b0310 JW0302 Uncharacterized protein ykgHIntegral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN450.104CYT0-0.200913-220i21-42o186-209iUnknown225.47
SECD_ECOLIP0AG90
secD
b0408 JW0398 Protein-export membrane protein secDCell inner membrane Inner membrane (experimental). Integral inner membrane (experimental).PMID: 16079137 PMID: 15919657 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN250.158TMH01.57163-660i7-25o453-472i477-497o503-524i545-571o577-605iCytoplasmicMembrane1036.08
SECF_ECOLIP0AG93
secF
b0409 JW0399 Protein-export membrane protein secFIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN30.112TMH02.24012-660i24-43o142-163i170-191o197-217i248-266o272-297iCytoplasmicMembrane10
SECE_ECOLIP0AG96
secE

prlG

prlGb3981 JW3944 Preprotein translocase subunit SecEIntegral inner membrane (experimental).Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRP (exp)SECyesyesN480.137TMH07.80526-330o16-36i43-61o95-119iCytoplasmicMembrane107.85
SECG_ECOLIP0AG99
secG
b3175 JW3142 Protein-export membrane protein SecG (P12) (Preprotein translocase band 1 subunit)Inner membrane (experimental). Integral inner membrane (experimental).PMID: 15919657 PMID: 15919996Integral Inner Membrane BBinoutin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN290.153TMH011.5638-21Yn4-22c27/28o51-70iCytoplasmicMembrane1049.35
SECY_ECOLIP0AGA2
secY

prlA

prlAb3300 JW3262 Preprotein translocase subunit SecYIntegral inner membrane (experimental). Integral Membrane ProteinPMID: 15919996 PMID: 3004955Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN470.104CYT0-0.200913-10100i23-44o75-98i119-139o154-174i186-203o215-237i274-296o316-337i373-391o397-418iCytoplasmicMembrane10
CUSF_ECOLIP77214
cusF

cusX ylcC

cusX ylcCb0573 JW0562 Cation efflux system protein cusFPeriplasmicPMID: 12813074PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.444SpI228.68332-00Yn4-15c22/23oPeriplasmic10
YAHC_ECOLIP77219
yahC
b0317 JW0309 Uncharacterized protein yahCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN700.119TMH010.9451-45Yn4-15c23/24o33-49i61-80o86-104i109-127o133-152iCytoplasmicMembrane10
UHPT_ECOLIP0AGC0
uhpT
b3666 JW3641 Hexose phosphate transport proteinIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN530.105TMH00.582302-12120i28-45o65-85i97-117o123-148i160-183o189-210i260-278o298-318i327-345o351-380i392-411o423-446iCytoplasmicMembrane10
SLT_ECOLIP0AGC3
slt

sltY

sltYb4392 JW4355 Soluble lytic murein transglycosylase (EC 4.2.2.n1) (Exomuramidase) (Peptidoglycan lytic exotransglycosylase) (Slt70)Periplasmic Disulfide bridge (133 166) Disulfide bond-containing protein PMID: 25664544PMID: 1938883 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY280.803SpI2712.0915-00Yn11-22c27/28oPeriplasmic1010.89
MLTF_ECOLIP0AGC5
mltF

yfhD

yfhDb2558 JW2542 Membrane-bound lytic murein transglycosylase F (EC 4.2.2.n1) (Murein lyase F)Peripheral outer membrane protein.PMID: 18234673Peripheral outer membrane protein facing the periplasmF3N/ACell outer membrane | Peripheral membrane proteinPeriplasmicSECnoyesN330.227SpI303.07095-10Yn9-20c32/33oCytoplasmic9.2610.36
SMP_ECOLIP0AGC7
smp

ytjB

ytjBb4387 JW4350 Protein smpHas an aminoterminal stop transfer or signal sequence hydrophobic region and a second hydrophobic region near the C-terminus that may function as a stop transfer sequence; The protein was shown to fractionate with the bacterial membrane. No processing of tPMID: 2684780Integral Inner Membrane BBinoutin/outIM (predicted)Cell membrane | Peripheral membrane proteinMembrane anchoredSRPSECpseudogene (Ochman & Davalos, 2006)nonoN300.128SpI307.66045-11Yn13-24c29/30o164-184iCytoplasmicMembrane1063.29
ESSQ_ECOLIP77237
essQ

ydfS

ydfSb1556 JW5255 Lysis protein S homolog from lambdoid prophage QinIntegral Inner Membrane BBinoutin/inIM (predicted)Integral Membrane ProteinSRPSECProphage Integrase (EcoGene)yesnoN380.166CYT0-0.200913-21Yn7-15c20/21o36-58iUnknown217.06
RNFG_ECOLIP77285
rnfG

ydgP

ydgPb1631 JW1623 Electron transport complex protein rnfG Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/ACell inner membranePeriplasmicSECyesyesY250.427SpI2411.9411-00Yn9-17c24/25oUnknown2.542.9
YBET_ECOLIP77296
ybeT
b0647 JW0642 Uncharacterized protein ybeTOuter Membrane LipoproteinIN/AOuter membrane LipoproteinSECLOLnoyesN220.112SpII185.89834Pos+2=I10Yn8-17c21/22oUnknown249.04
SODC_ECOLIP0AGD1
sodC
b1646 JW1638 Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Bacteriocuprein)Periplasmic Disulfide bridge (74 169) PMID: 9405149 Disulfide bond-containing protein PMID: 25664544PMID: 8626323 PMID: 9405149 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.82SpI1916.2903-00Yn4-14c19/20oPeriplasmic10
SODF_ECOLIP0AGD3
sodB
b1656 JW1648 Superoxide dismutase [Fe] (EC 1.15.1.1)PeriplasmThe superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are E.coli proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm. PeriplasmicGN/ACytoplasmicSEC, non classicalyesyesN170.122CYT0-0.200913-000iPeriplasmic9.4477.73
SSTT_ECOLIP0AGE4
sstT

ygjU

ygjUb3089 JW3060 Serine/threonine transporter sstT (Na(+)/serine-threonine symporter)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN420.148TMH07.50816-880i18-36o42-72i84-106o149-166i187-209o215-240i290-315o327-352iCytoplasmicMembrane1015.42
XYLE_ECOLIP0AGF4
xylE
b4031 JW3991 D-xylose-proton symporter (D-xylose transporter)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN300.155TMH06.47202-1211Yn13-24c32/33o56-78i90-109o129-151i163-186o198-220i270-293o313-334i346-364o370-395i407-426o446-463iCytoplasmicMembrane10
YAFU_ECOLIP77354
yafU
b0218 JW0207 Putative inner membrane protein yafUIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECpseudogene (Uniprot)nonoN390.16TMH01.65262-220i21-38o44-64iCytoplasmicMembrane1073.08
HOKB_ECOLIP77494
hokB

ydcB

ydcBb4428 JW5225 Autoinducer 2 import system permease protein lsrC (AI-2 import system permease protein lsrC)Potentially toxic protein.PMID: 19121005Integral Inner Membrane BBinout/inN/AMembrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN260.105CYT0-0.200913-110o6-24iPeriplasmic9.9642.15
YBDJ_ECOLIP77506
ybdJ
b0580 JW0569 Uncharacterized protein ybdJIntegral Inner Membrane BBinoutin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN360.165TMH013.1416-21Yn7-25c33/34o49-70iCytoplasmicMembrane9.86
YDDL_ECOLIP77519
yddL
b1472 JW1468 Putative uncharacterized protein yddLPeriplasmicGN/APeriplasmicSECpseudogene (Ochman & Davalos, 2006)yesyesY220.898SpI2118.2436-00Yn5-16c21/22oOuterMembrane9.9348.14
YHHJ_ECOLIP0AGH1
yhhJ
b3485 JW5677 Inner membrane transport permease yhhJIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.231SpI401.57995-560i174-200o220-244i256-281o287-306i318-337o343-364iCytoplasmicMembrane106.97
SAPB_ECOLIP0AGH3
sapB
b1293 JW1286 Peptide transport system permease protein sapBIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN280.197TMH016.0324-560i9-29o82-101i113-141o175-193i245-263o283-305iCytoplasmicMembrane109.44
SAPC_ECOLIP0AGH5
sapC
b1292 JW1285 Peptide transport system permease protein sapCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.132TMH05.59827-560i31-54o99-123i144-169o189-208i220-241o261-283iCytoplasmicMembrane1010.97
PSTC_ECOLIP0AGH8
pstC

phoW

phoWb3727 JW3705 Phosphate transport system permease protein pstCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN30.109TMH04.7509-660i21-43o75-100i112-143o163-186i227-249o282-304iCytoplasmicMembrane10
RBSC_ECOLIP0AGI1
rbsC
b3750 JW3729 Ribose transport system permease protein rbsCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN580.121TMH06.39117-8100i23-43o49-68i75-93o99-119i126-144o170-190i221-240o252-270i275-292o298-316iCytoplasmicMembrane1018.02
YAGL_ECOLIP77607
yagL
b0278 JW0272 Uncharacterized protein yagLIntegral Inner Membrane BBoutin/outIM (predicted)CytoplasmicSRPSECProphage Integrase (EcoGene)nonoN320.112CYT0-0.200913-010i12-32oCytoplasmic8.9613.93
GTRA_ECOLIP77682
yfdG
b2350 JW2346 Bactoprenol-linked glucose translocase homolog from prophage CPS-53Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECProphage Integrase (EcoGene)nonoN380.341TMH011.761-440i12-30o36-55i67-85o91-110iCytoplasmicMembrane10
SPY_ECOLIP77754
spy
b1743 JW1732 Spheroplast protein YPeriplasmicPMID: 9068658PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY240.817SpI2320.7622-00Yn4-15c23/24oPeriplasmic10104.11
XYLH_ECOLIP0AGI4
xylH
b3568 JW3540 Xylose transport system permease protein xylHIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN510.13TMH04.89182-10110i25-45o65-83i90-107o113-132i139-156o176-196i217-235o241-259i290-306o326-357i369-385oCytoplasmicMembrane10
UBIA_ECOLIP0AGK1
ubiA

cyr

cyrb4040 JW4000 4-hydroxybenzoate octaprenyltransferase (EC 2.5.1.-) (4-HB polyprenyltransferase)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN360.118TMH06.00125-770i23-40o46-70i99-132o152-182i210-229o235-252i273-289oCytoplasmicMembrane10
YHHT_ECOLIP0AGM0
yhhT
b3474 JW5680 UPF0118 inner membrane protein yhhTIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN310.162TMH05.38885-880i12-31o37-55i62-87o133-163i197-215o221-246i253-275o295-315iCytoplasmicMembrane104.32
YICG_ECOLIP0AGM2
yicG
b3646 JW3621 UPF0126 inner membrane protein yicGIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN220.368CYT0-0.200913-770o6-23i30-52o64-82i89-109o115-136i148-167o173-191iCytoplasmicMembrane10
URAA_ECOLIP0AGM7
uraA
b2497 JW2482 Uracil permease (Uracil transporter)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN470.107TMH03.93213-12130i20-38o44-60i67-83o89-108i120-147o159-176i183-202o225-250i262-281o301-319i331-354o366-384i391-409oCytoplasmicMembrane10
LYSD_ECOLIP78285
rrrD

arrD ybcS

arrD ybcSb0555 JW0544 Probable lysozyme from lambdoid prophage DLP12 (EC 3.2.1.17) (Endolysin) (Lysis protein) (Muramidase)PeriplasmicGN/APeriplasmicSECProphage Integrase (EcoGene)nonoN220.149SpI214.27124-00Yn8-16c21/22oUnknown276.96
YAHH_ECOLIQ2EEQ3
yahH
b0322 JW5893 Putative uncharacterized protein yahHMembrane anchored by EchoLOCATIONIntegral Inner Membrane B-N/AMembrane anchoredSECpseudogene (Uniprot), pseudogene (Ecogene)nonoN420.264CYT0-0.200913-000iUnknown2
YNID_ECOLIQ2EES1
yniD
b4535 JW5911 Uncharacterized protein yniDSmall membrane protein detected experimentally.PMID: 19121005Integral Inner Membrane BBinoutin/outIM (predicted)Membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN310.1TMH04.22082-10Yn14-26c30/31oCytoplasmicMembrane9.82
YOHO_ECOLIQ2EES6
yohO
b4542 JW5354 UPF0387 membrane protein yohOSmall membrane protein detected experimentally PMID: 19121005Integral Inner Membrane BBinoutout/inN/ACell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN260.149TMH04.51604-110o6-31iCytoplasmicMembrane9.82
XANP_ECOLIP0AGM9
xanP

yicE

yicEb3654 JW3629 Xanthine permease XanPCell inner membrane Integral inner membrane (experimental).PMID: 16096267 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN660.123CYT0-0.200913-11110o36-58i70-89o128-146i158-181o193-212i219-240o260-280i350-372o378-396i408-427o439-458iCytoplasmicMembrane109.17
MLTC_ECOLIP0C066
mltC

yggZ

yggZb2963 JW5481 Membrane-bound lytic murein transglycosylase C (EC 4.2.2.n1) (Murein hydrolase C)Outer membrane Lipoprotein (experimental) Outer membrane protein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY240.3SpII1614.8852Pos+2=S00Yn5-15c20/21oUnknown217.78
PROP_ECOLIP0C0L7
proP
b4111 JW4072 Proline/betaine transporter (Proline porter II) (PPII)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN410.105CYT0-0.200913-12120i41-59o65-86i98-116o122-146i167-186o198-217i257-278o298-318i327-346o352-376i388-411o417-438iCytoplasmicMembrane10
MSCS_ECOLIP0C0S1
mscS

yggB

yggBb2924 JW2891 Small-conductance mechanosensitive channel Integral inner membrane (experimental). Integral inner membrane (experimental). Inner membrane (experimental). Integral inner membrane (experimental). Integral inner membrane (experimental). Integral Membrane Protein Integral Membrane Protein Integral Membrane ProteinPMID: 11266632 PMID: 12446901 PMID: 16079137 PMID: 15919657 PMID: 15919996 PMID: 12446901 PMID: 12767977 PMID: 12015316 PMID: 10202137Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN520.15TMH0-0.184901-330o24-45i65-85o97-125iCytoplasmicMembrane1042.06
YPFN_ECOLIQ2EET2
ypfN
b4547 JW2457 UPF0370 protein ypfNIntegral Inner Membrane BBinout/inIM (predicted)Cell membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN260.107CYT0-0.200913-110o6-24iUnknown271.1
YTJA_ECOLIQ2M5U1
ytjA
b4568 JW5891 UPF0391 membrane protein ytjAIntegral Inner Membrane BBinoutin/inN/ACell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.166TMH011.6664-21Yn4-14c19/20o29-49iCytoplasmicMembrane9.86
YSAB_ECOLIQ2M7M3
ysaB
b4553 JW3532 Uncharacterized lipoprotein ysaBOuter Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane LipoproteinSECLOLyesyesY270.418SpII1715.6662Pos+2=S00Yn5-16c26/27oUnknown236.16
MEPA_ECOLIP0C0T5
mepA
b2328 JW2325 Penicillin-insensitive murein endopeptidase (EC 3.4.24.-) (D-alanyl-D-alanine-endopeptidase) (DD-endopeptidase) Periplasmic Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2187143 Disulfide bridge (44 265;187 235;216 223) Disulfide bond-containing protein PMID: 25664544PMID: 14726535 PMID: 19953543 PMID: 19953543 PMID: 2187143 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECDSBA,DSBCyesyesY200.847SpI1922.606-00Yn4-14c19/20oPeriplasmic1016.62
DEGP_ECOLIP0C0V0
degP

htrA ptd

htrA ptdb0161 JW0157 Periplasmic serine endoprotease DegPPeripheral inner membrane protein facing the periplasm. Periplasmic SecB substrate. Substrate of periplasmic disulfide oxidoreductases DsbA. HtrA was not detected by the immunoblotting technique in the periplasm or in the fraction of soluble proteins but was found in the inner membrane. DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into 12 and 24-mer cages with large chambers that house the substrate for degradation or refolding. Size exclusion chromatography and electron microscopy show that the size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme. Identified DsbA substrate PMID: 19953543 PMID: 2180903 Disulfide bridges between 83 and 95 PMID: 12878036 Disulfide bridge (83 95) PMID: 12878036 Disulfide bond-containing protein PMID: 25664544PMID: 16352602 PMID: 14726535 PMID: 21370981 PMID: 19525348 PMID: 9083020 PMID: 21526129 PMID: 19953543 PMID: 2180903 PMID: 12878036 PMID: 12878036 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECBSECDSBAyesyesY270.841SpI2628.3128-00Yn4-19c26/27oPeriplasmic1031.01
EMTA_ECOLIP0C960
emtA

mltE sltZ ycgP

mltE sltZ ycgPb1193 JW5821 Endo-type membrane-bound lytic murein transglycosylase A (EC 4.2.2.n2) (Peptidoglycan lytic endotransglycosylase)Lipoprotein (experimental). Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY490.204SpII1518.8581Pos+2=S00Yn5-13c18/19oCytoplasmicMembrane9.8215.95
CPTA_ECOLIP0CB39
cptA

cptA yijP

cptA yijPb3955 JW3927 Phosphoethanolamine transferase CptA (EC 2.7.-.-)Cell inner membranePMID: 16079137Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN90.182TMH012.4414-550i20-39o45-63i70-91o120-139i151-169oCytoplasmicMembrane1026.69
YICS_ECOLIQ2M7X4
yicS
b4555 JW5965 Uncharacterized protein yicS Non secreted. Uknown location Disulfide bond-containing protein PMID: 25664544 PMID: 25664544 novel bacterial periplasmic protein with no homologues of known function PMID: 21317318PMID: 25664544 PMID: 25664544PeriplasmicGN/APeriplasmicSECyesyesN220.681SpI2110.9898-00Yn4-14c21/22oUnknown2.5
YOBH_ECOLIQ2MB16
yobH
b4536 JW5298 Uncharacterized protein yobHSignal peptide predicted, single transmembrane helice predicted which would be removed upon signal peptide cleavage. Putative outer membrane or exported. Taxonomy: Klebsiella pneumoniae.Integral Inner Membrane BBoutin/outN/AMembrane anchoredSRPSECnoyesN340.169TMH05.54741-10Yn10-20c24/25oCytoplasmicMembrane9.8257.93
UIDB_ECOLIP0CE44
uidB

gusB uidP

gusB uidPb1616 JW1608 Glucuronide carrier protein homologIntegral inner membrane (experimental). Integral Membrane ProteinPMID: 15919996 PMID: 15774881Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN610.197TMH05.96454-12120i12-35o41-60i81-101o113-136i148-170o182-201i230-257o263-284i296-316o322-343i376-393o405-427iCytoplasmicMembrane10
ACFD_ECOLIP0CK95
yghJ
b4466 JW5925 ECK2968Putative lipoprotein AcfD homologInner Membrane LipoproteinEN/ACell membrane | Lipid-anchorInner membrane lipoproteinSECLOLnoyesY230.283SpII2337.591Pos+2=D00Yn10-21c26/27oUnknown6.26
RCSC_ECOLIP0DMC5
rcsC
b2218 JW5917/JW5920 Sensor kinase protein RcsC (EC 2.7.13.3) (Capsular synthesis regulator component C)Cell inner membraneFusions of rcsC to phoA that resulted in PhoA+ strains demonstrated that RcsC is a transmembrane protein with a periplasmic N-terminal domain and cytoplasmic C-terminal domain.PMID: 15919996 PMID: 2404948Integral Inner Membrane BBinoutout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN360.159TMH03.16217-21Yn20-31c40/41o314-334iCytoplasmicMembrane10
SIRB2_ECOLIQ46755
sirB2

ychQ

ychQb1213 JW1204 Protein sirB2Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN390.138TMH06.48818-440o6-28i40-61o73-93i105-123oCytoplasmicMembrane1025.14
PBL_ECOLIQ46790
pbl
b2854 Putative peptidoglycan-binding-like proteinPeriplasmicGN/APeriplasmCytoplasmicSECpseudogene (Uniprot)nonoY250.548SpI242.39101-00Yn8-19c24/25oPeriplasmic9.4428.8
YGEQ_ECOLIQ46797
ygeQ
b2863 JW5461 Putative uncharacterized protein ygeQPeriplasmicGN/APeriplasmicSECpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoY370.449SpI3611.1359-00Yn20-31c36/37oUnknown268.19
RLPA_ECOLIP10100
rlpA
b0633 JW0628 Rare lipoprotein AOuter membrane Lipoprotein (experimental) Outer membrane protein (experimental). Septal ring protein. Peripheral inner membrane protein facing the cytoplasm (experimental). Outer membrane protein (experimental). Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005). Contains a SPOR domain. PMID: 15174130PMID: 15174130 PMID: 12488465 PMID: 15911532 PMID: 19880599 PMID: 20932056Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesN200.14SpII1719.3561Pos+2=T00Yn5-15c20/21oUnknown2.5
FADL_ECOLIP10384
fadL

ttr

ttrb2344 JW2341 Long-chain fatty acid transport protein (Outer membrane fadL protein) (Outer membrane flp protein)Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane. Outer Membrane Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinSubstrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 1987139PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 11266632 PMID: 15178802 PMID: 16079137 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 19953543 PMID: 1987139Outer Membrane b-barrel proteinHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSURA,SKPBAMyesyesY260.796SpI2516.4528-00Yn10-20c25/26oOuterMembrane101.16
IAP_ECOLIP10423
iap
b2753 JW2723 Alkaline phosphatase isozyme conversion protein (EC 3.4.11.-)Membrane associatedPMID: 3316184Peripheral inner membrane protein facing the periplasmF2N/AMembrane associated (Experimental)SECyesyesY250.707SpI2413.6553-00Yn9-20c24/25oUnknown4.925.51
NARK_ECOLIP10903
narK
b1223 JW1214 Nitrite extrusion protein 1 (Nitrite facilitator 1)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN600.17TMH00.305282-12120i38-62o74-92i104-124o130-158i178-197o217-234i255-278o290-308i320-339o351-377i404-425o437-457iCytoplasmicMembrane10
YGFX_ECOLIQ46824
ygfX

ygfX

ygfXb2896 JW2864 Uncharacterized protein ygfXCell inner membranePMID: 22239607Integral Inner Membrane BBpolyin/inIM (predicted)Membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN390.141TMH05.61025-120i12-32o38-54iCytoplasmicMembrane9.8233.04
YQGA_ECOLIQ46831
yqgA
b2966 JW2934 Uncharacterized protein yqgAIntegral Inner Membrane BBpolyout/outIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN200.108TMH03.98827-680o6-23i30-50o56-75i103-123o135-157i164-181o187-206i213-233oCytoplasmicMembrane10
UGPA_ECOLIP10905
ugpA
b3452 JW3417 sn-glycerol-3-phosphate transport system permease protein ugpAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN390.107TMH013.7961-660i12-31o78-100i112-130o157-181i206-228o267-288iCytoplasmicMembrane10
UGPE_ECOLIP10906
ugpE
b3451 JW3416 sn-glycerol-3-phosphate transport system permease protein ugpEIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN440.2TMH09.6685-670i7-31o81-105i112-133o145-166i187-210o216-238i250-272oCytoplasmicMembrane1028.1
NARI_ECOLIP11350
narI

chlI

chlIb1227 JW1218 Respiratory nitrate reductase 1 gamma chain (EC 1.7.99.4) (Cytochrome B-NR) (Nitrate reductase A subunit gamma) (Quinol-nitrate oxidoreductase subunit gamma) Integral inner membrane (experimental). Integral inner membrane (experimental). Integral Membrane ProteinUnder anaerobic conditions in the presence of nitrate, E. coli synthesizes the cytoplasmic membrane-bound quinol-nitrate oxidoreductase (nitrate reductase A; NarGHI) PMID: 12910261. Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. PMID: 12910261 In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. PMID: 19295650 When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. PMID: 2674654 The heterotrimeric NarGHI has an αβγ structure encoded by narGHI respectively. The α and β subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane γ subunit. PMID: 3371362 PMID: 3053688PMID: 11266632 PMID: 12910261 PMID: 15919996 PMID: 19295650 PMID: 2674654 PMID: 3371362 PMID: 3053688Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN190.113TMH011.8819-550o6-26i47-69o89-109i129-148o179-198iCytoplasmicMembrane10
FUCP_ECOLIP11551
fucP
b2801 JW2772 Uncharacterized protein yjeVIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN420.298TMH00.0692344-12120i21-41o61-85i92-111o117-134i155-177o210-228i262-282o294-315i327-345o351-373i385-405o411-431iCytoplasmicMembrane10
YGHD_ECOLIQ46832
yghD
b2968 JW2935 Putative general secretion pathway protein M-type yghDIntegral Inner Membrane BBoutin/outIM (predicted)Cell inner membraneMembrane anchoredSRPSECnoyesN430.102CYT0-0.200913-110i38-57oUnknown228.25
YGHE_ECOLIQ46833
yghE
b2969 Putative general secretion pathway protein L-type yghEIntegral Inner Membrane BBinoutin/outIM (predicted)Cell inner membraneCytoplasmicSRPSECpseudogene (Uniprot) nonoN380.296CYT0-0.200913-110i136-159oUnknown2
YGHF_ECOLIQ46834
yghF
b2970 JW5484 Putative general secretion pathway protein C-type yghFPeriplasmicGN/APeriplasmCytoplasmicSECnoyesN430.145SpI161.1016-00Yn2-9c14/15oCytoplasmicMembrane9.8236.27
DAMX_ECOLIP11557
damX

yhfB

yhfBb3388 JW3351 Protein damXSeptal ring. Septal ring protein. Peripheral inner membrane protein facing the cytoplasm (experimental). Contain a SPOR Domain. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23290046 PMID: 19880599 PMID: 21541338Integral Inner Membrane BBinoutin/outIM (predicted), Detected in IM FractionCell membrane | Single-pass membrane proteinMembrane anchoredSRPSECnoyesN680.101CYT0-0.200913-110i103-124oUnknown2
ARGO_ECOLIP11667
argO

yggA

yggAb2923 JW2890 Arginine exporter protein ArgOIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein(experimental)SRPSECyesyesN250.273TMH06.65864-660o6-26i38-61o67-88i109-127o147-170i182-199oCytoplasmicMembrane1018.85
FECA_ECOLIP13036
fecA
b4291 JW4251 Fe(3+) dicitrate transport protein fecA (Iron(III) dicitrate transport protein fecA)Outer membrane protein (experimental). Outer membrane. Outer membrane protein (experimental). Outer Membrane ss-barrel proteinSubstrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 2836368PMID: 19343722 PMID: 15911532 PMID: 12624733 PMID: 11266632 PMID: 11872840 PMID: 20932056 PMID: 19953543 PMID: 19953543 PMID: 2836368Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECSURA,SKPBAMnoyesY340.831SpI3318.814-00Yn19-29c33/34oOuterMembrane101.41
TREA_ECOLIP13482
treA

osmA

osmAb1197 JW1186 Periplasmic trehalase (EC 3.2.1.28) (Alpha,alpha-trehalase) (Alpha,alpha-trehalose glucohydrolase) Periplasmic Potential Skp substrate PMID: 19953543 PMID: 2671658 Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 2671658 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECSKPyesyesY310.702SpI3017.5054-00Yn13-26c30/31oPeriplasmic1070.34
YGHG_ECOLIQ46835
yghG
b2971 JW2938 Uncharacterized lipoprotein yghGOuter membrane LipoproteinBelongs to the GSP C family. Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIN/ACell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLnoyesY290.253SpII2417.6171Pos+2=A00Yn10-22c27/28oUnknown2.540.56
YGHS_ECOLIQ46843
yghS
b2985 JW5491 Uncharacterized ATP-binding protein yghSIntegral Inner Membrane BBinoutin/outIM (predicted)CytoplasmicSRPSECnoyesN330.133CYT0-0.200913-010i161-178oCytoplasmic8.9635.57
CSRD_ECOLIP13518
csrD

yhdA

yhdAb3252 JW3221 RNase E specificity factor CsrD (Regulator of CsrB and CsrC decay CsrD)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN330.172TMH05.84825-220i7-31o130-155iCytoplasmicMembrane9.824.27
CHIA_ECOLIP13656
chiA

yheB

yheBb3338 JW3300 Probable bifunctional chitinase/lysozyme [Includes: Chitinase (EC 3.2.1.14); Lysozyme (EC 3.2.1.17)]Cytoplasm; Periplasm. Periplasm Can be fully secreted when the cryptic T2S system is expressed. PMID: 10760150 Disulfide bridge (628 673) Disulfide bond-containing protein PMID: 25664544PMID: 11118204 PMID: 10760150 PMID: 25664544Periplasmic, Extra-cellularG, XN/APeriplasmPeriplasmic (Experimental)SECT2SnoyesY250.672SpI2420.7505-00Yn11-20c24/25oPeriplasmic10
NHAA_ECOLIP13738
nhaA

ant

antb0019 JW0018 Na(+)/H(+) antiporter nhaA (Sodium/proton antiporter nhaA) Integral inner membrane (experimental). Integral inner membrane (experimental).Structure of a Na+/H+ antiporter. A negatively charged ion funnel opens to the cytoplasm and ends in the middle of the membrane at the putative ion-binding site PMID: 15988517.PMID: 11266632 PMID: 15988517 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN340.227TMH07.93195-11110i12-39o59-79i91-114o126-145i154-175o181-200i207-238o258-276i288-312o324-351i363-380oCytoplasmicMembrane10
PROW_ECOLIP14176
proW
b2678 JW2653 Glycine betaine/L-proline transport system permease protein proWIntegral inner membrane (experimental).PMID: 10949305 PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN240.12CYT0-0.200913-660o97-118i125-143o149-171i192-219o271-291i303-320oCytoplasmicMembrane105.07
YGHT_ECOLIQ46844
yghT
b2986 JW2954 Uncharacterized ATP-binding protein yghTIntegral Inner Membrane BBinout/inIM (predicted)CytoplasmicSRPSECnoyesN360.139CYT0-0.200913-010o77-95iCytoplasmic8.9650.11
YGIZ_ECOLIQ46867
ygiZ
b3027 JW2995 Inner membrane protein ygiZIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.138TMH04.05338-330i12-29o54-72i84-103oCytoplasmicMembrane1027.07
YQEJ_ECOLIQ46943
yqeJ
b2848 JW5455 Uncharacterized protein yfbKIntegral Inner Membrane BBoutin/outN/AMembrane | Single-pass membrane proteinPeriplasmicSRPSECnoyesN280.216SpI276.43067-110i7-27oUnknown2
ZRAS_ECOLIP14377
zraS

hydH

hydHb4003 JW3967 Sensor protein zraS (EC 2.7.13.3)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN440.129TMH07.59471-220i12-35o202-222iCytoplasmicMembrane10
FECB_ECOLIP15028
fecB
b4290 JW4250 Fe(3+) dicitrate-binding periplasmic protein (Iron(III) dicitrate-binding periplasmic protein)PeriplasmicPMID: 2651410PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY220.843SpI218.42911-00Yn7-16c21/22oPeriplasmic9.998.95
FECD_ECOLIP15029
fecD
b4288 JW4248 Fe(3+) dicitrate transport system permease protein fecD (Iron(III) dicitrate transport system permease protein fecD)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN280.121TMH010.0321-77Yn4-19c31/32o55-76i85-103o109-126i138-158o226-256i268-290o296-315iCytoplasmicMembrane104.14
FECC_ECOLIP15030
fecC
b4289 JW4249 Fe(3+) dicitrate transport system permease protein fecC (Iron(III) dicitrate transport system permease protein fecC)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN310.15TMH07.31223-89Yn8-27c36/37o60-81i93-111o117-140i152-171o177-194i201-220o240-270i282-304o310-328iCytoplasmicMembrane10
YAIO_ECOLIQ47534
yaiO
b0358 JW0349 Uncharacterized protein yaiOTMBB-DB tool predicts this protein as an outer membrane beta barrel protein.PeriplasmicGN/APeriplasmicSECnoyesY200.841SpI1912.701-10Yn5-15c19/20oUnknown2.55.67
YFEK_ECOLIQ47702
yfeK
b2419 JW2412 Uncharacterized protein yfeKPeriplasmicGN/APeriplasmicSECnoyesY200.758SpI192.85333-00Yn4-15c19/20oUnknown2.577.15
YQJK_ECOLIQ47710
yqjK
b3100 JW3071 Uncharacterized protein yqjKIntegral Inner Membrane BBinoutin/outIM (predicted), Detected in IM FractionMembrane anchoredSRPSECyesyesN620.14CYT0-0.200913-110i47-65oUnknown230.6
YJDP_ECOLIQ6BEX5
yjdP
b4487 JW5890 Uncharacterized protein yjdPPeriplasmicGN/APeriplasmicSECnoyesY230.721SpI2214.9551-00Yn6-17c22/23oUnknown2.5
CSTA_ECOLIP15078
cstA

ybdC

ybdCb0598 JW0590 Uncharacterized protein yjcFIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN210.185TMH08.48745-18160i7-26o32-52i93-111o117-138i159-185o191-209i221-240o260-277i284-305o325-345i373-393o470-494i515-534o546-571i578-598o648-671iCytoplasmicMembrane10
FLK_ECOLIP15286
flk

div

divb2321 JW2318 Flagellar regulator flkIntegral Inner Membrane BBoutout/inIM (predicted)Cell inner membrane | Single-pass membrane proteinMembrane anchoredSRPSECyesyesN540.104CYT0-0.200913-110o313-330iCytoplasmicMembrane9.8261.56
DHG_ECOLIP15877
gcd
b0124 JW0120 Quinoprotein glucose dehydrogenase (EC 1.1.5.2) (Glucose dehydrogenase [pyrroloquinoline-quinone])Cell inner membrane, Periplasmic side Integral inner membrane (experimental). Cell inner membrane, Periplasmic side Integral Membrane Protein Integral Membrane Protein Integral Membrane ProteinPMID: 16079137 PMID: 15919996 PMID: 8509415 PMID: 8419307 PMID: 8554505Integral Inner Membrane BBpolyin/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane protein | Periplasmic sideIntegral Membrane Protein (Experimental)SRPSECyesyesN430.121TMH021.0845-550i12-35o41-58i65-81o87-106i113-140oCytoplasmicMembrane9.8618.68
AROP_ECOLIP15993
aroP
b0112 JW0108 Aromatic amino acid transport protein AroP (General aromatic amino acid permease)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN440.121TMH09.5384-12120i20-41o47-63i84-105o125-142i154-176o196-220i241-263o283-300i332-353o359-379i400-422o428-446iCytoplasmicMembrane10
LDRD_ECOLIQ6BF25
ldrD
b4453 JW5966 Small toxic polypeptide ldrDPotentially toxic protein.PMID: 19121005Integral Inner Membrane BBinoutout/inIM (predicted)CytoplasmicSRPSECyesyesN210.155CYT0-0.200913-110o14-31iCytoplasmicMembrane9.82
LDRA_ECOLIQ6BF86
ldrA

ldrC

ldrCb4419 JW5957Small toxic polypeptide ldrA/ldrCPotentially toxic protein.PMID: 19121005Integral Inner Membrane BBinoutout/inIM (predicted)CytoplasmicSRPSECyesyesN210.125CYT0-0.200913-110o6-31iCytoplasmicMembrane9.82
LDRB_ECOLIQ6BF87
ldrB
b4421 JW5958 Small toxic polypeptide ldrBPotentially toxic protein.PMID: 19121005Integral Inner Membrane BBinoutout/inIM (predicted)CytoplasmicSRPSECyesyesN210.121CYT0-0.200913-110o14-31iCytoplasmicMembrane9.82
YMGG_ECOLIQ7DFV3
ymgG
b1172 JW5178 UPF0757 protein ymgGBelongs to the UPF0757 family. PMID: 18192383 PMID: 17787032Integral Inner Membrane BBpolyin/inN/AMembrane anchoredSRPSECnoyesY230.871SpI2223.9513-20Yn5-16c22/23oUnknown2.598.94
PANF_ECOLIP16256
panF
b3258 JW3226 Sodium/pantothenate symporter (Pantothenate permease)Integral inner membrane (experimental).Prediction of one very weak transmembrane region.PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN240.187TMH08.73514-13130o6-24i45-63o75-94i122-144o156-178i190-213o233-253i273-298o318-341i369-386o392-417i424-442o448-466iCytoplasmicMembrane10
HYCC_ECOLIP16429
hycC

hevC

hevCb2723 JW2693 Formate hydrogenlyase subunit 3 (FHL subunit 3) (Hydrogenase-3 component C)Cell inner membranePMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN320.168TMH011.9962-14160o6-26i33-58o78-96i108-124o130-146i153-173o197-218i230-251o257-280i292-312o332-349i370-395o415-435i456-480o500-521i587-605oCytoplasmicMembrane10
HYCD_ECOLIP16430
hycD

hevD

hevDb2722 JW2692 Formate hydrogenlyase subunit 4 (FHL subunit 4) (Hydrogenase 3 component D)Integral inner membrane (experimental).PMID: 1625581 PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN300.198CYT0-0.200913-880o6-27i62-85o97-119i131-149o169-188i224-242o248-273i285-306oCytoplasmicMembrane10
PHND_ECOLIP16682
phnD

psiD

psiDb4105 JW4066 Phosphonates-binding periplasmic proteinPeriplasmicPMID: 16751609PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.567SpI2616.4228-00Yn6-21c26/27oPeriplasmic10
PHNE_ECOLIP16683
phnE
b4104 JW4065 b4103Putative phosphonates transport system permease protein phnECell inner membranePMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECpseudogene (Ochman & Davalos, 2006)noyesN360.143TMH08.60214-660i12-33o75-99i120-143o179-197i206-224o230-250iCytoplasmicMembrane107.9
PHNP_ECOLIP16692
phnP
b4092 JW4053 Protein phnPPeriplasmicPMID: 8335257PeriplasmicGN/APeriplasmic (Experimental)SECyesyesN210.153CYT0-0.200913-00Yn3-10c15/16oCytoplasmic9.26
CYSP_ECOLIP16700
cysP
b2425 JW2418 Thiosulfate-binding proteinPeriplasmicPMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECyesyesY260.741SpI2514.3932-00Yn10-20c25/26oPeriplasmic1061.88
CYST_ECOLIP16701
cysU

cysT

cysTb2424 JW2417 Sulfate transport system permease protein cysTIntegral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN370.285TMH014.8684-750o12-37i58-84o104-125i137-156o242-263iCytoplasmicMembrane10117.73
FHUE_ECOLIP16869
fhuE
b1102 JW1088 FhuE receptor (Outer-membrane receptor for Fe(III)-coprogen, Fe(III)-ferrioxamine B and Fe(III)-rhodotrulic acid)Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel proteinOuter membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 3032906PMID: 10806384 PMID: 10806384 PMID: 3032906Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECBAMnoyesY370.821SpI3620.6615-00Yn19-30c36/37oOuterMembrane100.86
MREC_ECOLIP16926
mreC
b3250 JW3219 Rod shape-determining protein mreCCell inner membranePMID: 15612918Integral Inner Membrane BBoutin/outIM (predicted), Detected in IM FractionMembrane anchoredSRPSECnoyesN250.114TMH02.1577-10Yn15-23c28/29oUnknown235.38
CIRA_ECOLIP17315
cirA

cir feuA

cir feuAb2155 JW2142 Colicin I receptorInner membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer membrane protein (experimental). Outer Membrane ss-barrel protein Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 12624733 PMID: 10806384 PMID: 20932056 PMID: 10806384 PMID: 19953543 PMID: 19953543 PMID: 10430870 PMID: 25664544Outer Membrane b-barrel proteinHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECDSBA,SKPBAMyesyesY260.695SpI2511.8271-00Yn10-20c25/26oOuterMembrane1046.98
PTQC_ECOLIP17334
chbC

celB

celBb1737 JW1726 N,N'-diacetylchitobiose permease IIC component (EIIC-Chb) (PTS system N,N'-diacetylchitobiose-specific EIIC component)Integral inner membrane (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN280.124CYT0-0.200913-10100i35-55o86-107i114-133o145-167i187-207o235-258i301-320o332-352i359-384o404-429iCytoplasmicMembrane105.27
CYNX_ECOLIP17583
cynX
b0341 JW0332 Cyanate transport protein cynXCell inner membraneExhibites aberrant localization by microscopy in the absence of YidC.PMID: 22108384 PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCnoyesN550.147CYT0-0.200913-1211Yn2-10c18/19o34-55i67-85o91-113i125-147o153-173i201-224o230-251i263-281o287-309i321-339o354-372iCytoplasmicMembrane10
YFAA_ECOLIP17994
yfaA

pufY

pufYb2230 JW2224 Uncharacterized protein yfaAPeriplasmicGSoluble IM FractionPeriplasmicSECnoyesN310.233SpI305.42187-110i12-31oCytoplasmicMembrane9.8689.6
PNCB_ECOLIP18133
pncB
b0931 JW0914 Nicotinate phosphoribosyltransferase (NAPRTase) (EC 2.4.2.11)Periplasm.PncB is localized in the periplasm although it does not possess a signal peptide.PMID: 346557PeriplasmicGN/ACytoplasmCytoplasmicSEC, non classicalnoyesN610.11CYT0-0.200913-000iCytoplasmic9.9732.26
PGPA_ECOLIP18200
pgpA

yajN

yajNb0418 JW0408 Phosphatidylglycerophosphatase A (EC 3.1.3.27)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN450.137TMH013.1049-350i21-51o57-75i87-104o110-126i138-162oCytoplasmicMembrane1023
RSTB_ECOLIP18392
rstB

uspT

uspTb1609 JW1601 Sensor protein rstB (EC 2.7.13.3)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN320.112TMH02.20306-220o6-27i136-157oCytoplasmicMembrane1053.8
DMSB_ECOLIP18776
dmsB
b0895 JW0878 Anaerobic dimethyl sulfoxide reductase chain B (DMSO reductase iron-sulfur subunit)Peripheral inner membrane protein facing the cytoplasm or the periplasm. TAT piggyback; DmsB is co-exported bound to Tat substrate DmsA. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332.PMID: 3062312 PMID: 11389150 PMID: 9546395 PMID: 2170332Peripheral inner membrane protein facing the periplasmF2N/ACytoplasmicTAT piggybackyesyesN440.107CYT0-0.200913-000iUnknown5.4127.7
DMSA_ECOLIP18775
dmsA
b0894 JW5118 Anaerobic dimethyl sulfoxide reductase chain A (DMSO reductase) (EC 1.8.99.-)Periplasmic Peripheral inner membrane protein facing the cytoplasm or the periplasm. Cell membrane, Peripheral membrane protein, Cytoplasmic side TAT signal peptide, dmsABC complex. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD. PMID: 24093457 Fluorescence analysis of these YFP chimeras revealed a wide range of interactions such as the one between the Tat substrate dimethyl sulfoxide reductase (DmsA) and its dedicated proofreading chaperone DmsD. PMID: 20169075 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395. TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332. PMID: 3280546 PMID: 3062312 Interacts with DmsD, a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 17218314 PMID: 3062312 PMID: 11389150 PMID: 24093457 PMID: 20169075 PMID: 9546395 PMID: 2170332 PMID: 3280546 PMID: 20153451Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane | Peripheral membrane protein | Cytoplasmic sidePeriplasmic (Experimental)DmsDTAT,SECyesyesN460.537SpI455.24058-000iCytoplasmicMembrane1022.52
DMSC_ECOLIP18777
dmsC
b0896 JW0879 Anaerobic dimethyl sulfoxide reductase chain C (DMSO reductase anchor subunit)Integral inner membrane (experimental). Integral inner membrane. Peripheral inner membrane protein facing the cytoplasm or the periplasm. DMSO reductase subunit C,Periplasmic. Has a membrane bound anchor. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 PMID: 15919996PMID: 15919996 PMID: 3062312 PMID: 11389150 PMID: 9546395 PMID: 2170332Integral Inner Membrane BBpolyout/outIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN220.177TMH013.558-880o12-32i44-68o88-105i117-137o149-171i183-203o223-243i255-277oCytoplasmicMembrane10
GGT_ECOLIP18956
ggt
b3447 JW3412 Gamma-glutamyltranspeptidase (EC 2.3.2.2) [Cleaved into: Gamma-glutamyltranspeptidase large chain; Gamma-glutamyltranspeptidase small chain]Periplasm Periplasmic Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 1360205 PMID: 2570061 PMID: 25403562 PMID: 25403562PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECYfgMyesyesY260.723SpI2520.0548-00Yn10-21c25/26oPeriplasmic1036.63
PTOCB_ECOLIP19642
malX
b1621 JW1613 PTS system maltose- and glucose-specific EIICB component [Includes: Maltose and glucose permease IIC component (PTS system maltose- and glucose-specific EIIC component); Maltose- and glucose-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN440.116TMH07.8693-8100i21-42o62-89i96-116o136-161i182-205o295-313i320-337o343-362i369-391o397-419iCytoplasmicMembrane105.71
AGP_ECOLIP19926
agp
b1002 JW0987 Glucose-1-phosphatase (G1Pase) (EC 3.1.3.10)Periplasmic Periplasmic Disulfide bond-containing protein PMID: 25664544PMID: 2153660 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY230.88SpI2221.9324-00Yn5-16c22/23oPeriplasmic1062.1
TOLA_ECOLIP19934
tolA

cim excC lky

cim excC lkyb0739 JW0729 Protein tolAInner membrane (experimental). Membrane anchored The N-terminal coiled coil of YbgFvforms a stable trimer and controls the oligomeric status of YbgF. The stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein. PMID: 7744737 Disulfide bridge (363 388) Disulfide bond-containing protein PMID: 25664544PMID: 15919657 PMID: 20816983 PMID: 7744737 PMID: 25664544Integral Inner Membrane BBoutin/outIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SRPSECyesyesN430.131CYT0-0.200913-110i12-31oUnknown250.77
PTFBC_ECOLIP20966
fruA

ptsF

ptsFb2167 JW2154 PTS system fructose-specific EIIBC component (EIIBC-Fru) [Includes: Fructose-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (EIII-Fru) (PTS system fructose-specific EIIB component); Fructose permease IIC component (PTS system fructose-specIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN200.169CYT0-0.200913-990i230-256o268-291i303-329o349-368i389-410o430-451i463-482o488-507i519-551oCytoplasmicMembrane107.17
SPEA_ECOLIP21170
speA
b2938 JW2905 Biosynthetic arginine decarboxylase (ADC) (EC 4.1.1.19)Peripheral inner membrane protein facing the cytoplasm (potential). Periplasmic Peripheral inner membrane protein facing the cytoplasm (potential). PeriplasmTrigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 2198270 Selective disruption of the cell envelope and an assessment of Spea activity or immunoprecipitable SpeA in various fractions demonstrated its location between the cytoplasmic membrane and peptidoglycan layer. PMID: 3894328PMID: 19737520 PMID: 15919657 PMID: 2198270 PMID: 19766000 PMID: 3894328PeriplasmicGSoluble IM FractionPeriplasmCytoplasmicTFSEC, non classicalyesyesN270.129CYT0-0.200913-000iPeriplasmic1048
RNI_ECOLIP21338
rna

rnsA

rnsAb0611 JW0603 Ribonuclease I (RNase I) (EC 3.1.27.6) (Enterobacter ribonuclease) Periplasmic Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbA substrate PMID: 19953543 PMID: 2253883 Disulfide bridge (103 159) PMID: 14726535 PMID: 19953543 PMID: 2253883 PeriplasmicGN/APeriplasmCytoplasmPeriplasmic (Experimental)SECDSBA,DSBCyesyesY240.538SpI2515.6073-00Yn8-18c23/24oPeriplasmic1031.78
GLTP_ECOLIP21345
gltP
b4077 JW4038 Proton glutamate symport protein (Glutamate-aspartate carrier protein)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN310.123TMH011.2813-10100i7-27o47-72i84-106o160-181i202-224o236-264i293-311o323-350i362-385o391-409iCytoplasmicMembrane1019.34
YCIC_ECOLIP21365
yciC
b1255 JW1247 UPF0259 membrane protein yciCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN470.196TMH06.50025-660i20-41o77-104i116-136o142-166i187-212o218-239iCytoplasmicMembrane10
NMPC_ECOLIP21420
nmpC

phmA

phmAb0553 Putative outer membrane porin protein nmpCOuter Membrane ss-barrel proteinPMID: 337109Outer Membrane b-barrel proteinHN/AOuter Membrane ss-barrel protein(experimental)SECBAMpseudogene (Uniprot) yesnoY240.879SpI2325.1335-00Yn4-15c23/24oOuterMembrane1011.44
YCAD_ECOLIP21503
ycaD
b0898 JW0881 Uncharacterized MFS-type transporter ycaDIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN350.154TMH014.4924-1211Yn10-18c23/24o47-65i74-92o98-119i131-149o161-181i202-223o235-253i265-283o289-311i323-345o351-369iCytoplasmicMembrane10
KDPD_ECOLIP21865
kdpD
b0695 JW0683 Sensor protein kdpD (EC 2.7.13.3)Cell inner membrane Integral inner membrane (experimental).PMID: 1532388 PMID: 21605551 PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN460.149CYT0-0.200913-440i399-419o425-443i448-465o477-498iCytoplasmicMembrane1022.58
RECJ_ECOLIP21893
recJ
b2892 JW2860 Single-stranded-DNA-specific exonuclease recJ (EC 3.1.-.-)Integral Inner Membrane BBinoutin/outIM (predicted)CytoplasmicSRPSECyesyesN390.112CYT0-0.200913-010i188-206oCytoplasmic9.97
YCBB_ECOLIP22525
ycbB
b0925 JW0908 Probable L,D-transpeptidase YcbB (EC 2.-.-.-)Peripheral outer membrane protein facing the periplasm (experimental).YcbB forms direct meso- diaminopimelate (DAP-DAP, or 3-3) crosslinks within the peptidoglycan.PMID: 23832002Peripheral outer membrane protein facing the periplasmF3N/AMembrane | Single-pass membrane proteinPeriplasmicSECyesyesY310.448SpI3010.8337-00Yn11-22c30/31oPeriplasmic9.8463.67
LIVM_ECOLIP22729
livM
b3456 JW3421 High-affinity branched-chain amino acid transport system permease protein livM (LIV-I protein M)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN210.39SpI2011.5293-1010Yn9-20c25/26o44-61i91-108o114-132i137-156o162-184i191-209o262-283i313-336o348-373i385-403oCytoplasmicMembrane10
TNAB_ECOLIP23173
tnaB

trpP

trpPb3709 JW5619/JW5622 Low affinity tryptophan permeaseIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN250.14TMH016.6914-11110i12-32o38-61i82-105o125-143i155-174o194-219i226-246o282-310i322-341o347-369i381-406oCytoplasmicMembrane105.98
MGLC_ECOLIP23200
mglC
b2148 JW2135 Galactoside transport system permease protein mglCIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyin/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN330.106TMH05.37465-980i12-31o43-64i105-129o135-155i175-201o227-248i269-299o311-330iCytoplasmicMembrane10
HYFB_ECOLIP23482
hyfB

yffF

yffFb2482 JW2467 Hydrogenase-4 component B (EC 1.-.-.-)Integral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpolyout/outIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECnoyesN570.166TMH07.57984-16160o6-27i39-62o82-103i110-129o135-154i166-185o205-224i245-267o273-296i303-326o332-363i383-405o425-452i473-496o532-551i652-671oCytoplasmicMembrane10
FECR_ECOLIP23485
fecR
b4292 JW4252 Protein fecRInner membraneThe protein is oriented such that residues 1 to 85 are in the cytoplasm, residues 86 to 100 form a transmembrane segment, and residues 101 to 317 are in the periplasm where the C-terminus can interact with the N-domain of the outer membrane protein FecA.PMID: 9573190 PMID: 12057967Integral Inner Membrane B-IM (predicted-detected)PeriplasmIntegral Membrane ProteinSRPSECnoyesN350.136CYT0-0.200913-000iPeriplasmic10
ECOT_ECOLIP23827
eco

eti

etib2209 JW2197 EcotinPeriplasmic Periplasmic Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)SECnoyesY210.889SpI2016.5012-00Yn4-15c20/21oPeriplasmic1085.11
PHOQ_ECOLIP23837
phoQ
b1129 JW1115 Sensor protein phoQ (EC 2.7.13.3)Integral inner membrane (experimental). Sensor protein PhoQ (P23837) PMID: 23359663 PMID: 15919996 Integral Inner Membrane BBinout/inIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECyesyesN230.287TMH06.14229-21Yn17-28c33/34o194-216iCytoplasmicMembrane1020.04
YFEA_ECOLIP23842
yfeA
b2395 JW5391 Uncharacterized protein YfeAIntegral inner membrane (experimental).PMID: 15919996Integral Inner Membrane BBpoly