entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refsnterm_ctermsuboci_fullsublocisubloci_iimbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
RIR1_ECOLIP00452
nrdA

dnaF

dnaFb2234 JW2228 Ribonucleoside-diphosphate reductase 1 subunit alpha (EC 1.17.4.1) (Protein B1) (Ribonucleoside-diphosphate reductase 1 R1 subunit) (Ribonucleotide reductase 1)Peripheral inner membrane protein facing the cytoplasm (Experimental) (Experimental)Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490PMID: 23230279 PMID: 9395490Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicTFyesyesN400.104CYT0-0.200913-00="0"iCytoplasmic9.9763.38
AK2H_ECOLIP00562
metL

metM

metMb3940 JW3911 Bifunctional aspartokinase/homoserine dehydrogenase 2 (Aspartokinase II/homoserine dehydrogenase II) (AKII-HDII) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.3)]Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicyesyesN240.117CYT0-0.200913-00="0"iCytoplasmicMembrane9.82
PPB_ECOLIP00634
phoA
b0383 JW0374 Alkaline phosphatase (APase) (EC 3.1.3.1)Periplasm (Probable) Periplasmic (Experimental) (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544PMID: 3533724 PMID: 7022451 PMID: 3009404 PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAnoyesY220.408SpI213.05238-00Yn5-16c21/22oPeriplasmic1078.02
BTUE_ECOLIP06610
btuE
b1710 JW1700 Vitamin B12 transport periplasmic protein BtuE (EC 1.-.-.-) Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmCytoplasmicSEC, non classicalyesyesN370.111CYT0-0.200913-00="0"iPeriplasmic1099.42
RL31_ECOLIP0A7M9
rpmE
b3936 JW3907 50S ribosomal protein L31 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544PMID: 21541338RibosomalrrN/ACytoplasmicyesyesN310.105CYT0-0.200913-00="0"iCytoplasmic9.2666.19
FLHD_ECOLIP0A8S9
flhD

flbB

flbBb1892 JW1881 Transcriptional activator FlhDNucleoid (Experimental) Disulfide bridge (65 65) PMID: 23138451NucleoidNNN/ACytoplasmCytoplasmicnoyesN220.156CYT0-0.200913-00="0"iCytoplasmic9.9788.42
LEP_ECOLIP00803
lepB
b2568 JW2552 Signal peptidase I (SPase I) (EC 3.4.21.89) (Leader peptidase I)Integral inner membrane (Experimental) (Experimental)Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased. Lep was shown to be targeted to the membrane by the SRP pathway. PMID: 8985168 Disulfide bridge (171 177) PMID: 21778229 PMID: 15919996 PMID: 8985168 out/outIntegral Inner Membrane BBIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinYidCyesyesN220.131SpII203.04951Pos+2=V20Yn5-16c21/22oCytoplasmicMembrane1027.67
ASPG2_ECOLIP00805
ansB
b2957 JW2924 L-asparaginase 2 (EC 3.5.1.1) (L-asparaginase II) (L-ASNase II) (L-asparagine amidohydrolase II) (Colaspase)Periplasmic (Experimental) (Experimental) Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (99 127) PMID: 8434007 Disulfide bond-containing protein PMID: 25664544PMID: 387570 PMID: 2407723 PMID: 25403562 PMID: 8434007PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.82SpI2219.7315-10Yn7-17c22/23oPeriplasmic1049.18
SYI_ECOLIP00956
ileS

ilvS

ilvSb0026 JW0024 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase) (IleRS)Translation unit. Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN610.113CYT0-0.200913-00="0"iCytoplasmic10
SYGA_ECOLIP00960
glyQ

glyS(A)

glyS(A)b3560 JW3531 Glycyl-tRNA synthetase alpha subunit (EC 6.1.1.14) (Glycine--tRNA ligase alpha subunit) (GlyRS)Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmCytoplasmicTFnoyesN570.112CYT0-0.200913-00="0"iCytoplasmic9.9759.14
YEDF_ECOLIP0AA31
yedF
b1930 JW1915 UPF0033 protein yedF Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN240.136CYT0-0.200913-00="0"iUnknown290.76
YAJD_ECOLIP0AAQ2
yajD
b0410 JW0400 Uncharacterized protein yajD Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN380.105CYT0-0.200913-00="0"iUnknown2108.15
YBEL_ECOLIP0AAT9
ybeL
b0643 JW0638 Uncharacterized protein ybeL Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN220.103CYT0-0.200913-00="0"iCytoplasmic8.9695.86
LAMB_ECOLIP02943
lamB

malB

malBb4036 JW3996 Maltoporin (Lambda receptor protein) (Maltose-inducible porin)Outer membrane protein (Probable) Outer membrane protein (Experimental) Outer Membrane ss-barrel protein (Experimental) (Experimental)Potential pubstrate of periplasmic chaperones Skp. Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000). Experimentally verified trimeric beta-barrel. N-terminal region is in periplasm. Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 346375 Disulfide bridges between 47 and 63 PMID: 1988451 Disulfide bond-containing protein PMID: 25664544PMID: 16372265 PMID: 15911532 PMID: 10806384 PMID: 16079137 PMID: 20932056 PMID: 346375 PMID: 1988451Outer Membrane b-barrel proteinHHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSKP,SURA,DEGPBAMyesyesY260.795SpI2520.7922-00Yn8-18c25/26oOuterMembrane1054.55
FIMA1_ECOLIP04128
fimA

pilA

pilAb4314 JW4277 Type-1 fimbrial protein, A chain (Type-1A pilin)Cell surface appendage (Experimental)Fim operon (class γ1); Fimbrial Subunit. Experimentally verified signal peptide PMID: 2858471 Disulfide bridge (44 84) Disulfide bond-containing protein PMID: 25664544PMID: 2858471Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUyesyesY240.78SpI2325.7568-10Yn5-16c23/24oExtracellular10
TYRB_ECOLIP04693
tyrB
b4054 JW4014 Aromatic-amino-acid aminotransferase (ARAT) (AROAT) (EC 2.6.1.57) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN100.122CYT0-0.200913-00="0"iCytoplasmic1038.03
LIVK_ECOLIP04816
livK
b3458 JW3423 Leucine-specific-binding protein (L-BP) (LS-BP)Periplasmic (Experimental) (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 22030350 PMID: 8061609PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.817SpI2317.7516-00Yn7-18c23/24oPeriplasmic1078.71
YBGS_ECOLIP0AAV6
ybgS
b0753 JW0736 Uncharacterized protein ybgS Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY250.845SpI2432.7676-00Yn6-17c24/25oPeriplasmic9.84
YCAR_ECOLIP0AAZ7
ycaR
b0917 JW0900 UPF0434 protein ycaR Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN560.117CYT0-0.200913-00="0"iUnknown2
YMGD_ECOLIP0AB46
ymgD
b1171 JW5177 Uncharacterized protein ymgD Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECnoyesY200.656SpI1914.7848-00Yn4-14c19/20oUnknown2.586.15
FEPA_ECOLIP05825
fepA

fep feuB

fep feuBb0584 JW5086 Ferrienterobactin receptor (Enterobactin outer-membrane receptor) (Probable) Outer membrane (Probable) Outer membrane protein (Experimental) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544PMID: 11266632 PMID: 9886293 PMID: 20932056 PMID: 10806384 PMID: 10529396Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECSKPBAMyesyesY230.836SpI2211.5401-00Yn7-17c22/23oOuterMembrane1011.34
GSHR_ECOLIP06715
gor
b3500 JW3467 Glutathione reductase (GR) (GRase) (EC 1.8.1.7) (Experimental) Disulfide bridge (42 47) PMID: 8061609PMID: 8061609CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN240.239CYT0-0.200913-00="0"iCytoplasmic1067.27
FHUA_ECOLIP06971
fhuA

tonA

tonAb0150 JW0146 Uncharacterized protein yohNOuter membrane protein (Experimental) (Probable) Outer membrane (Probable) Outer Membrane ss-barrel protein (Experimental)SecB substrate; Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 3079747 Disulfide bridge (351 362;725 731) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 10806384 PMID: 11266632 PMID: 9856937 PMID: 3079747Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)SECBSECSURA,SKPBAMyesyesY340.805SpI3320.4987-00Yn16-24c33/34oOuterMembrane107.42
ACYP_ECOLIP0AB65
yccX
b0968 JW5131 Acylphosphatase (EC 3.6.1.7) (Acylphosphate phosphohydrolase) Disulfide bridge (5 49) PMID: 17134700CytoplasmicAAN/ACytoplasmicnoyesN280.119CYT0-0.200913-00Yn4-11c16/17oCytoplasmic9.26103.06
GLRX3_ECOLIP0AC62
grxC

yibM

yibMb3610 JW3585 Glutaredoxin-3 (Grx3)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (12 15) PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicyesyesN300.112CYT0-0.200913-00="0"iUnknown279.59
NRDH_ECOLIP0AC65
nrdH

ygaN

ygaNb2673 JW2648 Glutaredoxin-like protein nrdH Disulfide bridge (11 14) CytoplasmicAAN/ACytoplasmicnoyesN570.105CYT0-0.200913-00="0"iUnknown2102.78
YDCA_ECOLIP0ACW4
ydcA
b1419 JW1416 Uncharacterized protein ydcA Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECnoyesY210.761SpI2012.9463-00Yn4-15c20/21oUnknown2
USHA_ECOLIP07024
ushA
b0480 JW0469 Protein ushA [Includes: UDP-sugar hydrolase (EC 3.6.1.45) (UDP-sugar diphosphatase) (UDP-sugar pyrophosphatase); 5'-nucleotidase (5'-NT) (EC 3.1.3.5)]Periplasmic (Experimental) Disulfide bridge (258 275) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECnoyesY260.748SpI2511.3752-00Yn7-18c25/26oPeriplasmic1077.35
PPA_ECOLIP07102
appA
b0980 JW0963 Periplasmic AppA protein [Includes: Phosphoanhydride phosphohydrolase (EC 3.1.3.2) (pH 2.5 acid phosphatase) (AP); 4-phytase (EC 3.1.3.26)]Periplasmic (Experimental) Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544PMID: 8387749PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBCyesyesY230.793SpI2213.6965-00Yn3-13c22/23oPeriplasmic1066.26
TDH_ECOLIP07913
tdh
b3616 JW3591 L-threonine 3-dehydrogenase (EC 1.1.1.103) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAIM (predicted)CytoplasmCytoplasmicyesyesN250.103CYT0-0.200913-00="0"iCytoplasmic1050.47
ILVB_ECOLIP08142
ilvB
b3671 JW3646 Acetolactate synthase isozyme 1 large subunit (AHAS-I) (EC 2.2.1.6) (Acetohydroxy-acid synthase I large subunit) (ALS-I) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN450.118CYT0-0.200913-00="0"iCytoplasmic9.9729.18
YHEV_ECOLIP0ADW8
yheV
b4551 JW3312 Uncharacterized protein yheV Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN240.14CYT0-0.200913-00="0"iUnknown2
BCP_ECOLIP0AE52
bcp
b2480 JW2465 Putative peroxiredoxin bcp (EC 1.11.1.15) (Bacterioferritin comigratory protein) (Thioredoxin reductase) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN510.123CYT0-0.200913-00="0"iCytoplasmic8.96
CREA_ECOLIP0AE91
creA

yjjD

yjjDb4397 JW4360 Protein creA Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECDSBAyesyesY220.8SpI2114.9398-00Yn6-16c21/22oUnknown2.5
YJCO_ECOLIP0AF56
yjcO
b4078 JW4039 Uncharacterized protein yjcO Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY180.752SpI1713.9637-00Yn4-13c17/18oUnknown2.582.96
FIMF_ECOLIP08189
fimF
b4318 JW4281 Protein fimFCell surface appendage (Experimental)Fim operon (class γ1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (38 78) Disulfide bond-containing protein PMID: 25664544PMID: 15372038Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY230.871SpI2217.2366-00Yn6-17c22/23oExtracellular9.72
FIMG_ECOLIP08190
fimG
b4319 JW4282 Protein fimGCell surface appendage (Experimental)Fim operon (class γ1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (39 77) Disulfide bond-containing protein PMID: 25664544PMID: 15372038Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY240.773SpI2318.1281-10Yn7-18c23/24oExtracellular9.6573.47
FOLC_ECOLIP08192
folC

dedC

dedCb2315 JW2312 Bifunctional protein folC [Includes: Folylpolyglutamate synthase (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) (Tetrahydrofolate synthase); Dihydrofolate synthase (EC 6.3.2.12)] Disulfide bridge (354 401) CytoplasmicAAN/ACytoplasmicyesyesN260.113CYT0-0.200913-00="0"iCytoplasmic9.9784.67
CPDB_ECOLIP08331
cpdB
b4213 JW4171 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC 3.1.3.6) (EC 3.1.4.16)Periplasm (Probable) Periplasm (Experimental) Periplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 3005231 PMID: 15911532 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECSURAnoyesY200.787SpI1914.5002-00Yn4-14c19/20oPeriplasmic1082.97
PSIF_ECOLIP0AFM4
psiF
b0384 JW5054 Phosphate starvation-inducible protein psiF Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY220.376SpI2113.709-10Yn5-16c21/22oUnknown244.48
THIO2_ECOLIP0AGG4
trxC

yfiG

yfiGb2582 JW2566 Thioredoxin-2 (Trx-2) (EC 1.8.1.8) (Protein-disulfide reductase) Disulfide bridge (64 67) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN700.102CYT0-0.200913-00="0"iCytoplasmic1092.23
YJJA_ECOLIP18390
yjjA
b4360 JW5795 Uncharacterized protein yjjA (Protein P-18) Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY260.86SpI2523.6068-00Yn8-19c25/26oUnknown2.5100.08
AK3_ECOLIP08660
lysC

apk

apkb4024 JW3984 Lysine-sensitive aspartokinase 3 (EC 2.7.2.4) (Aspartate kinase III) (AKIII) (Lysine-sensitive aspartokinase III) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN430.206CYT0-0.200913-00="0"iUnknown4.9
PT1_ECOLIP08839
ptsI
b2416 JW2409 Phosphoenolpyruvate-protein phosphotransferase (EC 2.7.3.9) (Phosphotransferase system, enzyme I)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 2644649 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmCytoplasmicyesyesN150.115CYT0-0.200913-00="0"iCytoplasmic1090.19
ARGT_ECOLIP09551
argT
b2310 JW2307 Lysine-arginine-ornithine-binding periplasmic protein (LAO-binding protein)Periplasmic (Experimental)This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 9298647 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.595SpI2219.5206-00Yn4-14c22/23oPeriplasmic10
GLTB_ECOLIP09831
gltB

aspB

aspBb3212 JW3179 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) (Glutamate synthase subunit alpha) (GLTS alpha chain) (NADPH-GOGAT)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicnoyesN480.135CYT0-0.200913-00="0"iCytoplasmic9.97
CSGC_ECOLIP52107
csgC
b1043 JW1026 Curli assembly protein CsgCUniprot mentions an 8aa signal peptide but Signal P prediction is for a "typical" 22 residue peptide Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmPeriplasmicSECnoyesN220.179SpI222.07115-00Yn4-16c21/22oUnknown2.568.8
RZOD_ECOLIP58041
rzoD
b4510 JW5080 Outer membrane lipoprotein Rz1 from lambdoid prophage DLP12 Disulfide bridge (29 29) Outer Membrane LipoproteinIIN/AOuter membrane LipoproteinSECLOLProphage Integrase (EcoGene)nonoY200.187SpII1919.9508Pos+2=T00Yn8-18c22/23oUnknown2147.46
LEUC_ECOLIP0A6A6
leuC
b0072 JW0071 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) (Alpha-IPM isomerase) (IPMI) (Isopropylmalate isomerase) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN410.123CYT0-0.200913-00="0"iCytoplasmic9.9770.18
DAPF_ECOLIP0A6K1
dapF
b3809 JW5592 Diaminopimelate epimerase (DAP epimerase) (EC 5.1.1.7) Disulfide bridge (73 217) CytoplasmicAAIM (predicted)CytoplasmCytoplasmicyesyesN190.106CYT0-0.200913-00="0"iCytoplasmic9.9717.3
DSBB_ECOLIP0A6M2
dsbB

roxB ycgA

roxB ycgAb1185 JW5182 Disulfide bond formation protein B (Disulfide oxidoreductase)Integral inner membrane (Experimental) Alpha-helcal transmembrane protein (Experimental) Integral inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC PMID: 22108384. The crystal structure revealed a transmembrane (TM) four-helix-bundle structure of DsbBPMID: 18406599. DsbB oxidizes the periplasmic protein DsbA, which in turn oxidizes the cysteines in other periplasmic proteins to make disulfide bondsPMID: 8430071. Gene fusion approaches were used to show that (i) DsbB is a membrane protein which spans the membrane four times and (ii) both the N- and C-termini of the protein are in the cytoplasmPMID: 7957076. The structure of DsbB revealed four transmembrane helices and one short horizontal helix juxtaposed with Cys130 in the mobile periplasmic loopPMID: 17110337. Disulfide bridge (41 44;104 130) PMID: 10064586PMID: 18406599 PMID: 17110337 PMID: 8430071 PMID: 7957076 PMID: 15919996in/inIntegral Inner Membrane BBIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPYidCyesyesN250.286TMH04.8983-44="0"i12-33o45-64i71-89o145-166iCytoplasmicMembrane1015.79
FLGI_ECOLIP0A6S3
flgI

fla FIX flaM

fla FIX flaMb1080 JW1067 Flagellar P-ring protein (Basal body P-ring protein)Periplasmic (Experimental) (Experimental)P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 12730325 PMID: 15838024PeriplasmicGGN/APeriplasmBacterial flagellum basal bodyPeriplasmic (Experimental)flagellumSRP (exp)SECDSBAFlanoyesY200.783SpI1915.6262-00Yn4-14c19/20oPeriplasmic1063.67
RZOR_ECOLIP58042
rzoR
b4528 JW5213 Outer membrane lipoprotein Rz1 from lambdoid prophage Rac Disulfide bridge (29 29) Outer Membrane LipoproteinIIN/ACell outer membrane | Lipid-anchorOuter membrane LipoproteinSECLOLProphage Integrase (EcoGene)nonoY200.189SpII1920.8844Pos+2=T00Yn8-18c22/23oUnknown288.14
YEBY_ECOLIP64506
yebY
b1839 JW1828 Uncharacterized protein yebY Identified DsbA substrate PMID: 19953543PeriplasmicGGN/APeriplasmicSECDSBAyesyesY210.862SpI2019.6801-10Yn5-12c20/21oUnknown2.535.83
YFIR_ECOLIP64548
yfiR
b2603 JW2584 Uncharacterized protein yfiR Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY230.684SpI2213.4131-00Yn7-17c22/23oUnknown6.5821.98
GLRX1_ECOLIP68688
grxA

grx

grxb0849 JW0833 Glutaredoxin-1 (Grx1) Disulfide bridge (11 14) CytoplasmicAAN/ACytoplasmicyesyesN180.204CYT0-0.200913-00="0"iUnknown2
GSH1_ECOLIP0A6W9
gshA

gsh-I

gsh-Ib2688 JW2663 Glutamate--cysteine ligase (EC 6.3.2.2) (Gamma-ECS) (GCS) (Gamma-glutamylcysteine synthetase) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN210.149CYT0-0.200913-00="0"iCytoplasmic9.9726.48
HSLO_ECOLIP0A6Y5
hslO

yrfI

yrfIb3401 JW5692 33 kDa chaperonin (Heat shock protein 33) (HSP33)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (230 232;263 266) PMID: 10764757PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1IM (predicted)CytoplasmCytoplasmicyesyesN630.125CYT0-0.200913-00="0"iCytoplasmic1088.87
MSRA_ECOLIP0A744
msrA

pms

pmsb4219 JW4178 Peptide methionine sulfoxide reductase msrA (Protein-methionine-S-oxide reductase) (EC 1.8.4.11) (Peptide-methionine (S)-S-oxide reductase) (Peptide Met(O) reductase)Cytoplasm (Experimental) YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300 Disulfide bridge (52 199); (199 207) PMID: 17272300CytoplasmicAAN/ACytoplasmicyesyesN160.11CYT0-0.200913-00="0"iUnknown285.08
MSRB_ECOLIP0A746
msrB

yeaA

yeaAb1778 JW1767 Peptide methionine sulfoxide reductase msrB (EC 1.8.4.12) (Peptide-methionine (R)-S-oxide reductase)Cytoplasm (Experimental)Fluorescent fusions of YeaA have been monitored via fluorescence microscopy. YeaA was found to be located evenly throughout cytoplasm. Disulfide bond-containing protein PMID: 25664544PMID: 17272300CytoplasmicAAN/ACytoplasmicyesyesN620.122CYT0-0.200913-00="0"iCytoplasmic9.9796.83
NAGB_ECOLIP0A759
nagB

glmD

glmDb0678 JW0664 Glucosamine-6-phosphate deaminase (EC 3.5.99.6) (GlcN6P deaminase) (GNPDA) (Glucosamine-6-phosphate isomerase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (219 219) PMID: 8240271PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1IM (predicted)CytoplasmicyesyesN320.107CYT0-0.200913-00="0"iCytoplasmic8.9628.22
YCGJ_ECOLIP76001
ycgJ
b1177 JW1166 Uncharacterized protein ycgJ Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY230.404SpI228.58297-00Yn6-16c24/25oUnknown2.577.92
YNJH_ECOLIP76227
ynjH
b1760 JW1749 Uncharacterized protein ynjH Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECnoyesY190.569SpI1811.8884-00Yn4-14c18/19oCytoplasmicMembrane9.8627.32
MSRC_ECOLIP76270
msrC

yebR

yebRb1832 JW1821 Protein yebR Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN200.13CYT0-0.200913-00="0"iUnknown257.22
FLHE_ECOLIP76297
flhE
b1878 JW1867 Flagellar protein flhEPart of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECFlayesyesY170.511SpI168.24229-00Yn3-14c26/27oUnknown2.568.14
PYRI_ECOLIP0A7F3
pyrI
b4244 JW4203 Aspartate carbamoyltransferase regulatory chainCytoplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 3912513 PMID: 8740179CytoplasmicAAN/ACytoplasmic (Experimental)yesyesN370.104CYT0-0.200913-00="0"iUnknown2116.96
RECA_ECOLIP0A7G6
recA

lexB recH rnmB tif umuB zab

lexB recH rnmB tif umuB zabb2699 JW2669 Protein recA (Recombinase A)Peripheral inner membrane protein facing the cytoplasm (Probable)Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 2228969Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCytoplasmCytoplasmicTFnoyesN30.103CYT0-0.200913-00="0"iCytoplasmic1088.16
RS12_ECOLIP0A7S3
rpsL

strA

strAb3342 JW3304 30S ribosomal protein S12 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544PMID: 21541338RibosomalrrSoluble IM FractionCytoplasmicnoyesN270.105CYT0-0.200913-00="0"iCytoplasmic1084.22
TPX_ECOLIP0A862
tpx

yzzJ

yzzJb1324 JW1317 Thiol peroxidase (EC 1.11.1.-) (Scavengase P20)Peripheral inner membrane protein facing the cytoplasm (Experimental) Periplasmic (Experimental)Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. PMID: 7499381 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 7499381 PMID: 9298646Peripheral inner membrane protein facing the cytoplasmF1F1N/APeriplasmPeriplasmic (Experimental)yesyesN330.123CYT0-0.200913-00="0"iPeriplasmic10
OMPA_ECOLIP0A910
ompA

con tolG tut

con tolG tutb0957 JW0940 Outer membrane protein A (Outer membrane protein II*)Outer membrane protein (Experimental) Outer Membrane (Probable) (Potential) Outer Membrane ss-barrel protein (Experimental) (Experimental)Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544PMID: 10806384 PMID: 9808047 PMID: 7813480 PMID: 16079137 PMID: 21778229 PMID: 20932056 PMID: 19953543 PMID: 9298646 PMID: 24717922Outer Membrane b-barrel proteinHHSoluble IM FractionCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)TFSECSKP,SURA,DEGP,DSBABAMyesyesY220.872SpI2128.1613-00Yn5-16c21/22oOuterMembrane1010.56
ALF1_ECOLIP0A991
fbaB

dhnA

dhnAb2097 JW5344 Fructose-bisphosphate aldolase class 1 (EC 4.1.2.13) (Fructose-biphosphate aldolase class I) (FBP aldolase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmCytoplasmicnoyesN640.115CYT0-0.200913-00="0"iUnknown24.72
FUR_ECOLIP0A9A9
fur
b0683 JW0669 Ferric uptake regulation protein (Ferric uptake regulator)Nucleoid (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23138451NucleoidNNN/ACytoplasmCytoplasmicyesyesN370.121CYT0-0.200913-00="0"iCytoplasmic10109.55
G3P1_ECOLIP0A9B2
gapA
b1779 JW1768 Glyceraldehyde-3-phosphate dehydrogenase A (GAPDH-A) (EC 1.2.1.12)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCytoplasmCytoplasmicTFyesyesN210.121CYT0-0.200913-00="0"iCytoplasmic9.9773.46
ACEA_ECOLIP0A9G6
aceA

icl

iclb4015 JW3975 Isocitrate lyase (ICL) (Isocitrase) (Isocitratase) (EC 4.1.3.1)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmCytoplasmicyesyesN300.126CYT0-0.200913-00="0"iCytoplasmic1042.87
DLDH_ECOLIP0A9P0
lpdA

lpd

lpdb0116 JW0112 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes) (Glycine cleavage system L protein)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 6352260 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (45 50) Disulfide bond-containing protein PMID: 25664544PMID: 16079137 PMID: 6352260 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane proteinPeripheral membrane proteinCytoplasmic (Experimental)TFyesyesN230.176CYT0-0.200913-00="0"iCytoplasmic1079.07
YKFB_ECOLIP77162
ykfB
b0250 JW0239 Uncharacterized protein ykfB Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECProphage Integrase (EcoGene)nonoY240.716SpI2317.8556-00Yn11-19c23/24oUnknown2.559.81
RNFG_ECOLIP77285
rnfG

ydgP

ydgPb1631 JW1623 Electron transport complex protein rnfG Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/ACell inner membranePeriplasmicSECyesyesY250.427SpI2411.9411-00Yn9-17c24/25oUnknown2.542.9
TRXB_ECOLIP0A9P4
trxB
b0888 JW0871 Thioredoxin reductase (TRXR) (EC 1.8.1.9) Disulfide bridge (136 139) PMID: 8114095PeriplasmicGGN/ACytoplasmCytoplasmicSECyesyesN260.317SpI256.69011-00Yn9-17c25/26oCytoplasmic10104.59
THIO_ECOLIP0AA25
trxA

fipA tsnC

fipA tsnCb3781 JW5856 Thioredoxin-1 (Trx-1)Peripheral inner membrane protein facing the cytoplasm (Experimental) (Experimental)Trigger factor-sensitive aggregation. Disulfide bridge (33 36) PMID: 10489448 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10489448Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicTFnoyesN200.119CYT0-0.200913-00="0"iCytoplasmic9.2689.01
DSBE_ECOLIP0AA86
dsbE

ccmG yejQ

ccmG yejQb2195 JW2183 Thiol:disulfide interchange protein dsbE (Cytochrome c biogenesis protein CcmG)Peripheral inner membrane protein facing the periplasm Periplasmic with N-terminal membrane anchor (Experimental) Periplasmic with N-terminal membrane anchor (Experimental)The CcmG protein was shown to be membrane bound, facing the periplasm with its C-terminal, hydrophilic domain. PMID: 9537397 PMID: 16364305 Disulfide bridge (80 83) PMID: 9537397 PMID: 16364305in/outIntegral Inner Membrane BBN/ACell inner membrane | Single-pass membrane protein | Periplasmic sidePeriplasmic with N-terminal membrane anchor (Experimental)SRPSECyesyesN290.264SpI278.59712-10Yn6-21c30/31oCytoplasmicMembrane1069.04
EFEO_ECOLIP0AB24
efeO

ycdO

ycdOb1018 JW1003 UPF0409 protein ycdOPeriplasm (Probable) Periplasmic (Experimental) (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 16551627 PMID: 12107143 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY270.767SpI2618.2235-00Yn9-18c26/27oPeriplasmic1042.79
CDD_ECOLIP0ABF6
cdd
b2143 JW2131 Cytidine deaminase (EC 3.5.4.5) (Cytidine aminohydrolase) (CDA)One very weak transmembrane reg ion pred ict ion. Disulfide bond-containing protein PMID: 25664544CytoplasmicAAIM (predicted)CytoplasmicyesyesN260.113CYT0-0.200913-00="0"iUnknown242.24
YKGA_ECOLIP77601
ykgA
b0300 JW5037 Putative HTH-type transcriptional regulator ykgANucleoid (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23138451NucleoidNNN/ACytoplasmicpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN560.1CYT0-0.200913-00="0"iCytoplasmic9.9714.3
RL362_ECOLIQ2EEQ2
rpmJ2

ykgO

ykgOb4506 JW5034 50S ribosomal protein L36 2Translation, Ribosomal. Expressed during stationary phase in minimal medium (at protein level). Disulfide bond-containing protein PMID: 25664544RibosomalrrN/A-noyesN380.12CYT0-0.200913-00="0"iUnknown2136.42
YICS_ECOLIQ2M7X4
yicS
b4555 JW5965 Uncharacterized protein yicS Disulfide bond-containing protein PMID: 25664544 PMID: 25664544 novel bacterial periplasmic protein with no homologues of known function PMID: 21317318PeriplasmicGGN/APeriplasmicSECyesyesN220.681SpI2110.9898-00Yn4-14c21/22oUnknown2.5
NRFA_ECOLIP0ABK9
nrfA
b4070 JW4031 Cytochrome c-552 (EC 1.7.2.2) (Ammonia-forming cytochrome c nitrite reductase) (Cytochrome c nitrite reductase)Periplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 793493PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.665SpI2614.744-00Yn11-21c26/27oPeriplasmic1025.45
NRFB_ECOLIP0ABL1
nrfB

yjcI

yjcIb4071 JW4032 Cytochrome c-type protein nrfBPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 15280383PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY330.498SpI256.62799-00Yn7-18c23/24oPeriplasmic9.8416.12
NAPB_ECOLIP0ABL3
napB

yejY

yejYb2203 JW5367 Diheme cytochrome c napBPeriplasm (Probable) Periplasm (Experimental) Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 10548535 PMID: 17130127 PMID: 11844760PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY280.825SpI2714.6891-00Yn9-20c27/28oPeriplasmic10
SFMA_ECOLIP0ABW5
sfmA
b0530 JW0519 Sfm fimbrial protein, A chain (Type-1A pilin)Potential fimbrial sfm operon (class γ1); Fimbrial Subunit. Disulfide bridge (41 81) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUyesyesY230.832SpI2221.4596-00Yn6-17c22/23oExtracellular1083.3
FUMA_ECOLIP0AC33
fumA
b1612 JW1604 Fumarate hydratase class I, aerobic (Fumarase) (EC 4.2.1.2)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicyesyesN570.116CYT0-0.200913-00="0"iCytoplasmic9.9737.19
GLRX2_ECOLIP0AC59
grxB
b1064 JW1051 Glutaredoxin-2 (Grx2) Disulfide bridge (9 12) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN330.104CYT0-0.200913-00="0"iCytoplasmic8.9665.99
NIFU_ECOLIP0ACD4
nifU

icsU yfhN

icsU yfhNb2529 JW2513 NifU-like proteinPeripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicyesyesN200.105CYT0-0.200913-00="0"iCytoplasmic9.2697.36
MBHM_ECOLIP0ACE0
hybC
b2994 JW2962 Hydrogenase-2 large chain (HYD2) (EC 1.12.99.6) (Membrane-bound hydrogenase 2 large subunit) (NiFe hydrogenase)Peripheral inner membrane protein facing the periplasm (Experimental) Periplasmic (Experimental)The protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system piggyback. PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544PMID: 3516690 PMID: 8772179 PMID: 9738917Peripheral inner membrane protein facing the periplasmF2F2Soluble IM FractionCell membrane | Peripheral membrane proteinPeriplasmic (Experimental)TAT piggybackyesyesN340.139CYT0-0.200913-00="0"iCytoplasmicMembrane1028.87
OXYR_ECOLIP0ACQ4
oxyR

momR mor

momR morb3961 JW3933 Hydrogen peroxide-inducible genes activator (Morphology and auto-aggregation control protein)Nucleoid (Experimental) Disulfide bridge (180 259) PMID: 12015987 Disulfide bridge (199 208) PMID: 9497290PMID: 23138451NucleoidNNSoluble IM FractionCytoplasmicyesyesN220.192CYT0-0.200913-00="0"iCytoplasmic9.9716.97
IVY_ECOLIP0AD59
ivy

ykfE

ykfEb0220 JW0210 Inhibitor of vertebrate lysozymePeriplasmic (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544PMID: 11278658PeriplasmicGGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECyesyesY290.849SpI2816.6514-00Yn13-23c28/29oPeriplasmic1027.17
AMPH_ECOLIP0AD70
ampH

yaiH

yaiHb0376 JW5052 Penicillin-binding protein AmpHInner membrane (Probable)Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein PMID: 25664544PMID: 22001512Peripheral inner membrane protein facing the cytoplasmF1F1N/APeriplasmicSECSURAyesyesY220.613SpI2116.801-00Yn5-16c21/22oPeriplasmic9.7641.35
LIVJ_ECOLIP0AD96
livJ
b3460 JW3425 Leu/Ile/Val-binding protein (LIV-BP)Periplasmic (Experimental) (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.832SpI2320.6668-00Yn7-18c23/24oPeriplasmic1096.06
OSME_ECOLIP0ADB1
osmE

anr

anrb1739 JW1728 Osmotically-inducible lipoprotein E (Activator of ntr-like gene protein)Lipoprotein (Experimental) Outer membrane protein (Experimental) Outer membrane Lipoprotein (Experimental)Proven signal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 10806384Outer Membrane LipoproteinIISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY240.207SpII2020.0226Pos+2=T10Yn7-18c23/24oUnknown298.73
YGGN_ECOLIP0ADS9
yggN
b2958 JW2925 Uncharacterized protein yggNPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535PMID: 14726535PeriplasmicGGN/APeriplasmic(experimental)SECDSBAyesyesY210.797SpI2021.2471-00Yn6-16c20/21oUnknown2.580.23
YGIB_ECOLIP0ADT2
ygiB
b3037 JW5927 UPF0441 protein ygiBPeripheral inner membrane protein facing the cytoplasm (Experimental)YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein PMID: 25664544PMID: 20545840 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCytoplasmicyesyesN360.178SpII369.72422Pos+2=E00Yn21-32c37/38oPeriplasmic9.83
AHPC_ECOLIP0AE08
ahpC
b0605 JW0598 Alkyl hydroperoxide reductase subunit C (EC 1.11.1.15) (Alkyl hydroperoxide reductase protein C22) (Peroxiredoxin) (SCRP-23) (Sulfate starvation-induced protein 8) (SSI8) (Thioredoxin peroxidase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (47 47;166 166) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicyesyesN490.111CYT0-0.200913-00="0"iCytoplasmic1090.35
DSBA_ECOLIP0AEG4
dsbA

dsf ppfA

dsf ppfAb3860 JW3832 Thiol:disulfide interchange protein DsbAPeriplasmic (Experimental) (Experimental)Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential β-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 15838024 PMID: 13129941PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SRP (exp)SECyesyesY200.75SpI1916.7987-00Yn4-15c19/20oPeriplasmic1071.65
DSBC_ECOLIP0AEG6
dsbC

xprA

xprAb2893 JW2861 Thiol:disulfide interchange protein DsbCPeriplasm (Probable) Periplasmic (Experimental)Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential β-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544PMID: 8168498 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY210.778SpI2021.5074-00Yn4-15c20/21oPeriplasmic1082.33
HDEA_ECOLIP0AES9
hdeA

yhhC yhiB

yhhC yhiBb3510 JW3478 Chaperone-like protein hdeA (10K-S protein)Periplasm (Probable) Periplasmic (Experimental) (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544PMID: 10623550 PMID: 17085547 PMID: 8455549 PMID: 25403562PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECYfgMyesyesY220.698SpI2116.385-10Yn4-15c21/22oUnknown2.5108.01
HDEB_ECOLIP0AET2
hdeB

yhhD yhiC

yhhD yhiCb3509 JW5669 Protein hdeB (10K-L protein)Periplasm (Probable) Periplasmic (Experimental) (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 8455550 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bond-containing protein PMID: 25664544PMID: 17085547 PMID: 8455550 PMID: 25403562PeriplasmicGGN/APeriplasmic (Experimental)SECYfgMyesyesY300.837SpI2916.8932-00Yn10-21c29/30oUnknown2.563.24
HDHA_ECOLIP0AET8
hdhA

hsdH

hsdHb1619 JW1611 7-alpha-hydroxysteroid dehydrogenase (7-alpha-HSDH) (EC 1.1.1.159) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN480.2CYT0-0.200913-00="0"iCytoplasmic9.2668.03
HISJ_ECOLIP0AEU0
hisJ
b2309 JW2306 Histidine-binding periplasmic protein (HBP)Periplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY230.792SpI2220.7158-10Yn4-14c22/23oPeriplasmic10
PROX_ECOLIP0AFM2
proX

proU

proUb2679 JW2654 Glycine betaine-binding periplasmic protein (Experimental) Periplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Identified DsbA substrate PMID: 19953543 PMID: 3305496 Disulfide bridge (157 163) Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 3305496PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECSURA,DSBAyesyesY220.863SpI2116.9579-00Yn5-16c21/22oPeriplasmic1052.14
UGPB_ECOLIP0AG80
ugpB
b3453 JW3418 sn-glycerol-3-phosphate-binding periplasmic protein ugpBPeriplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 3062310 Disulfide bond-containing protein PMID: 25664544PMID: 3062310PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.804SpI2317.1867-00Yn7-18c23/24oPeriplasmic1064.05
SLT_ECOLIP0AGC3
slt

sltY

sltYb4392 JW4355 Soluble lytic murein transglycosylase (EC 4.2.2.n1) (Exomuramidase) (Peptidoglycan lytic exotransglycosylase) (Slt70)Periplasmic (Experimental) Disulfide bridge (133 166) Disulfide bond-containing protein PMID: 25664544PMID: 1938883PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY280.803SpI2712.0915-00Yn11-22c27/28oPeriplasmic1010.89
SODC_ECOLIP0AGD1
sodC
b1646 JW1638 Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Bacteriocuprein)Periplasmic (Experimental) Disulfide bridge (74 169) PMID: 9405149 Disulfide bond-containing protein PMID: 25664544PMID: 8626323PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.82SpI1916.2903-00Yn4-14c19/20oPeriplasmic10
MEPA_ECOLIP0C0T5
mepA
b2328 JW2325 Penicillin-insensitive murein endopeptidase (EC 3.4.24.-) (D-alanyl-D-alanine-endopeptidase) (DD-endopeptidase)Periplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2187143 Disulfide bridge (44 265;187 235;216 223) Disulfide bond-containing protein PMID: 25664544PMID: 2187143PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBA,DSBCyesyesY200.847SpI1922.606-00Yn4-14c19/20oPeriplasmic1016.62
DEGP_ECOLIP0C0V0
degP

htrA ptd

htrA ptdb0161 JW0157 Periplasmic serine endoprotease DegPPeripheral inner membrane protein facing the periplasm (Probable) Periplasmic (Experimental) (Experimental)SecB substrate. Substrate of periplasmic disulfide oxidoreductases DsbA. HtrA was not detected by the immunoblotting technique in the periplasm or in the fraction of soluble proteins but was found in the inner membrane. DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into 12 and 24-mer cages with large chambers that house the substrate for degradation or refolding. Size exclusion chromatography and electron microscopy show that the size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme. Identified DsbA substrate PMID: 19953543 PMID: 2180903 Disulfide bridges between 83 and 95 PMID: 12878036 Disulfide bridge (83 95) PMID: 12878036 Disulfide bond-containing protein PMID: 25664544PMID: 9083020 PMID: 2180903 PMID: 12878036PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECBSECDSBAyesyesY270.841SpI2628.3128-00Yn4-19c26/27oPeriplasmic1031.01
GATZ_ECOLIP0C8J8
gatZ
b2095 JW2082 D-tagatose-1,6-bisphosphate aldolase subunit gatZPeripheral inner membrane protein facing the cytoplasm (Experimental)Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCytoplasmicTFyesyesN330.318CYT0-0.200913-00="0"iCytoplasmic8.966.6
EFTU1_ECOLIP0CE47
tufA
b3339 JW3301 Elongation factor Tu 1 (EF-Tu 1) (P-43)Peripheral inner membrane protein facing the cytoplasm (By similarity) Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) (Experimental)Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. PMID: 7525272 PMID: 7021545 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451 Disulfide bond-containing protein PMID: 25664544PMID: 20133608 PMID: 2022614 PMID: 775340 PMID: 7525272 PMID: 7021545 PMID: 20153451Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmCell membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN470.197CYT0-0.200913-00="0"iCytoplasmic1099.39
NADA_ECOLIP11458
nadA

nicA

nicAb0750 JW0733 Quinolinate synthase A (EC 2.5.1.72) Disulfide bridge (291 294) CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN580.105CYT0-0.200913-00="0"iCytoplasmic1034.82
TREA_ECOLIP13482
treA

osmA

osmAb1197 JW1186 Periplasmic trehalase (EC 3.2.1.28) (Alpha,alpha-trehalase) (Alpha,alpha-trehalose glucohydrolase)Periplasmic (Experimental) Potential Skp substrate PMID: 19953543 PMID: 2671658 Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PMID: 2671658PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECSKPyesyesY310.702SpI3017.5054-00Yn13-26c30/31oPeriplasmic1070.34
CHIA_ECOLIP13656
chiA

yheB

yheBb3338 JW3300 Probable bifunctional chitinase/lysozyme [Includes: Chitinase (EC 3.2.1.14); Lysozyme (EC 3.2.1.17)]Cytoplasm; Periplasm (Probable) Periplasm (Probable)Can be fully secreted when the cryptic T2S system is expressed. PMID: 10760150 Disulfide bridge (628 673) Disulfide bond-containing protein PMID: 25664544PMID: 11118204 PMID: 10760150Periplasmic, Extra-cellularG, XG,XN/APeriplasmPeriplasmic (Experimental)SECT2SnoyesY250.672SpI2420.7505-00Yn11-20c24/25oPeriplasmic10
FUMB_ECOLIP14407
fumB
b4122 JW4083 Fumarate hydratase class I, anaerobic (Fumarase) (EC 4.2.1.2) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN320.117CYT0-0.200913-00="0"iCytoplasmic9.9769.97
SELD_ECOLIP16456
selD

fdhB

fdhBb1764 JW1753 Selenide, water dikinase (EC 2.7.9.3) (Selenium donor protein) (Selenophosphate synthase) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN380.121CYT0-0.200913-00="0"iCytoplasmic8.9661.54
CIRA_ECOLIP17315
cirA

cir feuA

cir feuAb2155 JW2142 Colicin I receptorInner membrane protein (Experimental) Outer membrane protein (Experimental) (Potential) (Experimental) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 10806384 PMID: 20932056 PMID: 19953543 PMID: 10430870Outer Membrane b-barrel proteinHHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECDSBA,SKPBAMyesyesY260.695SpI2511.8271-00Yn10-20c25/26oOuterMembrane1046.98
AGP_ECOLIP19926
agp
b1002 JW0987 Glucose-1-phosphatase (G1Pase) (EC 3.1.3.10)Periplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 2153660 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY230.88SpI2221.9324-00Yn5-16c22/23oPeriplasmic1062.1
TOLA_ECOLIP19934
tolA

cim excC lky

cim excC lkyb0739 JW0729 Protein tolAInner membrane (Experimental) Membrane anchored (Experimental)The N-terminal coiled coil of YbgFvforms a stable trimer and controls the oligomeric status of YbgF. The stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein. PMID: 7744737 Disulfide bridge (363 388) Disulfide bond-containing protein PMID: 25664544PMID: 15919657 PMID: 7744737in/outIntegral Inner Membrane BBIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SRPSECyesyesN430.131CYT0-0.200913-11="0"i12-31oUnknown250.77
RNI_ECOLIP21338
rna

rnsA

rnsAb0611 JW0603 Ribonuclease I (RNase I) (EC 3.1.27.6) (Enterobacter ribonuclease)Periplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbA substrate PMID: 19953543 PMID: 2253883 Disulfide bridge (103 159) PMID: 2253883PeriplasmicGGN/APeriplasmCytoplasmPeriplasmic (Experimental)SECDSBA,DSBCyesyesY240.538SpI2515.6073-00Yn8-18c23/24oPeriplasmic1031.78
ECOT_ECOLIP23827
eco

eti

etib2209 JW2197 EcotinPeriplasmic (Probable) Periplasmic (Experimental) Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY210.889SpI2016.5012-00Yn4-15c20/21oPeriplasmic1085.11
OPPA_ECOLIP23843
oppA
b1243 JW1235 Periplasmic oligopeptide-binding proteinPeriplasmic (Experimental) (Experimental)SecB substrate. Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (297 443) Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 22030350PeriplasmicGGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECBSECyesyesY270.825SpI2618.3169-10Yn8-18c26/27oPeriplasmic1073.74
DPPA_ECOLIP23847
dppA
b3544 JW3513 Periplasmic dipeptide transport protein (Dipeptide-binding protein) (DBP)Periplasmic (Probable) (Potential) (Experimental) Periplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (34 262;450 463) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 19953543 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBA,SKPyesyesY290.638SpI2816.4393-00Yn13-23c28/29oPeriplasmic1085.98
PSPE_ECOLIP23857
pspE
b1308 JW1301 Thiosulfate sulfurtransferase PspE (TST) (EC 2.8.1.1) (Phage shock protein E)Periplasmic (Experimental) Periplasm (Potential)Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 9868784 Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 1712397PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECProphage TransposaseyesnoY200.9SpI1920.0627-00Yn5-15c19/20oUnknown2.5
AMY1_ECOLIP25718
malS
b3571 JW3543 Alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase)Periplasmic (Experimental) Disulfide bridge (57 75;121 537) PMID: 9268356 Disulfide bond-containing protein PMID: 25664544PMID: 1544897PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY180.764SpI1714.5348-00Yn3-11c17/18oPeriplasmic1025.14
END1_ECOLIP25736
endA
b2945 JW2912 Endonuclease-1 (EC 3.1.21.1) (Endonuclease I) (Endo I)Periplasm (Probable) Periplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 7867949 Disulfide bond-containing protein PMID: 25664544PMID: 4955462 PMID: 7867949PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAnoyesY230.883SpI2222.5344-00Yn5-16c22/23oUnknown5.0299.73
MNMA_ECOLIP25745
mnmA

asuE trmU ycfB

asuE trmU ycfBb1133 JW1119 tRNA-specific 2-thiouridylase mnmA (EC 2.8.1.-) Disulfide bridge (102 199) CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN230.288CYT0-0.200913-00="0"iCytoplasmic1068.02
LOIP_ECOLIP25894
yggG

yggG

yggGb2936 JW2903 Uncharacterized metalloprotease yggG (EC 3.4.24.-)Cell outer membrane (Probable) Outer membrane protein (Experimental) Disulfide bridge (53 108) PMID: 22491786PMID: 17909889 PMID: 22491786 PMID: 20932056Outer Membrane LipoproteinIIN/AOuter membrane LipoproteinSECLOLyesyesN350.146SpII1825.8287Pos+2=Q00Yn5-16c34/35oUnknown260.15
MLIC_ECOLIP28224
mliC

ydhA

ydhAb1639 JW1631 Membrane-bound lysozyme inhibitor of C-type lysozymeCell outer membrane (Probable) Disulfide bridge (37 102) PMID: 16937244PMID: 18369469Outer Membrane LipoproteinIIN/ACell outer membrane | Lipid-anchorOuter membrane LipoproteinSECLOLyesyesY230.288SpII177.61087Pos+2=S00Yn6-15c20/21oUnknown253.07
FIMD_ECOLIP30130
fimD
b4317 JW5780 Outer membrane usher protein fimDOuter membrane protein (Experimental) Outer membrane protein (Probable) Outer Membrane ss-barrel protein (Experimental)Fim operon (class γ1); Usher. Οuter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Identified in the outer membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 12842468 Disulfide bridge (855 877) PMID: 12488465 PMID: 15911532 PMID: 12842468Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)fimbriaeSECCUyesyesY380.415SpI371.20154-10Yn23-38c45/46oOuterMembrane10
THIC_ECOLIP30136
thiC
b3994 JW3958 Phosphomethylpyrimidine synthase (EC 4.-.-.-) (Hydroxymethylpyrimidine phosphate synthase) (HMP-P synthase) (HMP-phosphate synthase) (HMPP synthase) (Thiamine biosynthesis protein thiC)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Disulfide bond-containing protein PMID: 25664544CytoplasmicAAIM (predicted-detected)CytoplasmicyesyesN290.108CYT0-0.200913-00="0"iCytoplasmic9.9710.15
ARTI_ECOLIP30859
artI
b0863 JW0847 Putative ABC transporter arginine-binding protein 2Periplasm (Probable) Periplasmic (Probable) Periplasmic (Experimental) (Experimental) Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8801422 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 8801422 PMID: 15911532 PMID: 9298646 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.883SpI1918.5986-00Yn4-15c19/20oPeriplasmic1076.54
ARTJ_ECOLIP30860
artJ
b0860 JW0844 ABC transporter arginine-binding protein 1Periplasm (Probable) Periplasmic (Experimental) (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 8801423 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 8801422 PMID: 8801423 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY200.849SpI1918.9337-00Yn4-15c19/20oPeriplasmic1073.37
YEDD_ECOLIP31063
yedD
b1928 JW1913 Uncharacterized lipoprotein yedDOuter membrane Lipoprotein (Experimental) Outer membrane protein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532Outer Membrane LipoproteinIISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECDSBALOLnoyesY200.338SpII1512.6143Pos+2=A00Yn4-12c16/17oUnknown2.5130.89
YDEH_ECOLIP31129
ydeH

ydeG

ydeGb1535 JW1528 Diguanylate cyclase YdeH (DGC) (EC 2.7.7.65) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN240.128CYT0-0.200913-00="0"iCytoplasmic9.9736.55
POTF_ECOLIP31133
potF
b0854 JW0838 Putrescine-binding periplasmic proteinPeriplasmic (Experimental) Disulfide bridge (175 239) PMID: 9651355 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.731SpI2615.245-00Yn9-20c26/27oPeriplasmic1061.65
THIB_ECOLIP31550
thiB

tbpA yabL

tbpA yabLb0068 JW0067 Thiamine-binding periplasmic proteinPotential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmPeriplasmicSECSURAnoyesY190.685SpI1810.962-00Yn5-13c18/19oPeriplasmic1022.25
LPTD_ECOLIP31554
lptD

imp ostA yabG

imp ostA yabGb0054 JW0053 LPS-assembly protein lptD (Organic solvent tolerance protein)Outer membrane protein (Experimental) Outer Membrane (Probable) Outer Membrane ss-barrel protein (Experimental)The Protein-disulfide Isomerase DsbC Cooperates with SurA and DsbA in the Assembly of LptD. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (31 724;173 725) PMID: 15911532 PMID: 12724388 PMID: 16079137 PMID: 20932056 PMID: 9298646Outer Membrane b-barrel proteinHHSoluble IM FractionCell outer membraneOuter Membrane β-barrel protein (Experimental)SECDSBA,DSBC,SURA,SKPBAMyesyesY250.626SpI2418.6702-00Yn7-17c24/25oOuterMembrane10
RHAB_ECOLIP32171
rhaB
b3904 JW3875 Rhamnulokinase (EC 2.7.1.5) (Rhamnulose kinase) Disulfide bridge (68 222;353 370;413 417) CytoplasmicAAN/ACytoplasmicyesyesN210.191CYT0-0.200913-00="0"iCytoplasmic8.963.96
HTRE_ECOLIP33129
htrE
b0139 JW0135 Outer membrane usher protein htrE (Heat shock protein E)Putative chaperone–usher fimbrial operon (class γ4); Usher. Disulfide bridge (838 862) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMnoyesY300.677SpI296.72527-00="0"iOuterMembrane108.62
YEBF_ECOLIP33219
yebF
b1847 JW1836 Protein yebFExtra-cellular (Probable) Secreted and released (Experimental)Outer membrane proteins OmpF, OmpC, and OmpX are central to the YebF export mechanism. Identified DsbA substrate PMID: 19953543 PMID: 16369539 Disulfide bond-containing protein PMID: 25664544PMID: 16369539Extra-cellularXXN/ASecretedSecreted and released (Experimental)SECOMVnoyesY220.777SpI2120.4773-00Yn5-16c21/22oExtracellular10108.11
YEHB_ECOLIP33341
yehB
b2109 JW2096 Vitamin B12 transporter BtuB (Cobalamin receptor) (Outer membrane cobalamin translocator)Putative chaperone–usher fimbrial operon yeh (class γ4); Usher. Disulfide bridge (809 825) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMnoyesY230.679SpI2211.7505-00Yn7-18c22/23oOuterMembrane105.85
YEHD_ECOLIP33343
yehD
b2111 JW2098 Uncharacterized protein ybcHPutative chaperone–usher fimbrial operon yeh (class γ4); Fimbrial Subunit. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicfimbriaeSECnoyesY230.891SpI2224.5442-10Yn4-15c22/23oUnknown2.595.56
NAPA_ECOLIP33937
napA

yojC yojD yojE

yojC yojD yojEb2206 JW2194 Periplasmic nitrate reductase (EC 1.7.99.4)Periplasm (Probable) Periplasm (Experimental)TAT secretion system. Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 11844760PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)TATyesyesY320.617SpI3120.6365-00Yn13-23c31/32oPeriplasmic10
AHPF_ECOLIP35340
ahpF
b0606 JW0599 Alkyl hydroperoxide reductase subunit F (EC 1.8.1.-) (Alkyl hydroperoxide reductase F52A protein)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (345 348) PMID: 19766000 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCytoplasmicyesyesN350.108CYT0-0.200913-00="0"iCytoplasmicMembrane7.8876.12
DSBD_ECOLIP36655
dsbD

cutA2 cycZ dipZ

cutA2 cycZ dipZb4136 JW5734 Thiol:disulfide interchange protein dsbD (EC 1.8.1.8) (C-type cytochrome biogenesis protein cycZ) (Inner membrane copper tolerance protein) (Protein-disulfide reductase) (Disulfide reductase)Integral inner membrane (Experimental)DsbD is an inner membrane protein that is recognised and targeted to the Sec pathway by short, cleavable, N-terminal signal sequence. PMID: 15919996 Disulfide bridge (122 128;182 304;480 483) PMID: 15919996in/outIntegral Inner Membrane BBIM (predicted), Detected in IM FractionCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesY200.853SpI1910.1575-87Yn5-13c19/20o168-195i207-231o243-267i288-317o329-351i363-381o387-406iCytoplasmicMembrane10
ACNB_ECOLIP36683
acnB

yacI yacJ

yacI yacJb0118 JW0114 Aconitate hydratase 2 (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)Trigger factor-sensitive aggregation. PMID: 10585860 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10585860Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmic (Experimental)TFyesyesN420.124CYT0-0.200913-00="0"iCytoplasmic9.9790.34
RLMN_ECOLIP36979
rlmN

yfgB

yfgBb2517 JW2501 Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-) (23S rRNA m2A2503 methyltransferase)Trigger factor-sensitive aggregation. Disulfide bridge (118 355) CytoplasmicAAN/ACytoplasmCytoplasmicTFyesyesN580.101CYT0-0.200913-00="0"iCytoplasmic9.9760.19
BTUF_ECOLIP37028
btuF

yadT

yadTb0158 JW0154 Vitamin B12-binding proteinPeriplasm (Probable) Disulfide bridge (183 259) Disulfide bond-containing protein PMID: 25664544PMID: 11790740PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.751SpI2210.8439-00Yn8-18c22/23oPeriplasmic10
YADN_ECOLIP37050
yadN
b0141 JW0137 Uncharacterized fimbrial-like protein yadNPutative chaperone–usher fimbrial operon (class γ4); Fimbrial Subunit. Disulfide bridge (41 86) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY240.713SpI2317.9817-00Yn5-15c23/24oExtracellular9.6577.2
PTKB_ECOLIP37188
gatB
b2093 JW2077 Galactitol-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (EIIB-Gat) (PTS system galactitol-specific EIIB component)Peripheral inner membrane protein facing the cytoplasm (Potential) Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544PMID: 16963640 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmCytoplasmicnoyesY200.743SpI198.62134-00Yn5-14c19/20oCytoplasmic10
YHJJ_ECOLIP37648
yhjJ
b3527 JW3495 Protein yhjJPeriplasmic (Experimental) Potential Skp substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECSKPyesyesY250.722SpI2414.0569-00Yn8-19c24/25oUnknown2.583.47
EPTB_ECOLIP37661
eptB

yhjW

yhjWb3546 JW5660 Phosphoethanolamine transferase eptB (EC 2.7.-.-)Cell inner membrane (Probable) Integral inner membrane (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 15795227 PMID: 15919996in/outIntegral Inner Membrane BBIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesN380.136TMH011.408-55="0"i12-30o42-65i77-103o123-140i161-178oCytoplasmicMembrane10
YIBQ_ECOLIP37691
yibQ
b3614 JW5645 Uncharacterized protein yibQPeriplasmicGGN/APeriplasmicSECyesyesY240.782SpI2313.9145-10Yn8-18c23/24oUnknown2.514.82
WBBK_ECOLIP37751
yefI

yefI

yefIb2032 JW2017 Uncharacterized protein yefIPeripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN210.119CYT0-0.200913-00="0"iUnknown232.03
GLTI_ECOLIP37902
gltI

ybeJ yzzK

ybeJ yzzKb0655 JW5092 Glutamate/aspartate periplasmic-binding proteinPeriplasmic (Experimental) (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 8740179 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 8740179 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY230.775SpI2221.5903-00Yn7-17c22/23oPeriplasmic1093.99
YBGD_ECOLIP37909
ybgD
b0719 JW0709 Uncharacterized fimbrial-like protein ybgDPutative chaperone–usher fimbrial operon yeh (class π); Fimbrial Subunit. Disulfide bridge (42 81) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY240.878SpI2315.4399-00Yn7-18c23/24oExtracellular9.72
SFMF_ECOLIP38052
sfmF

ybcG

ybcGb0534 JW5072 Fimbrial-like protein sfmFPotential fimbrial sfm operon (class γ1); Fimbrial Subunit. Disulfide bridge (40 80) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY210.763SpI2016.3129-00Yn4-15c20/21oExtracellular9.7164.72
TORT_ECOLIP38683
torT

yccH

yccHb0994 JW0979 Periplasmic protein torTPeriplasmic (Experimental) (Experimental)Might be is co-translationally translocated through Sec translocon. PMID: 8576063 The precursor of TorT harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 Disulfide bond-containing protein PMID: 25664544PMID: 8576063 PMID: 24717922 PMID: 15838024PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SRP (exp)SECyesyesY190.789SpI1812.9296-00Yn3-14c18/19oPeriplasmic1012.65
ZNUA_ECOLIP39172
znuA

yebL yzzP

yebL yzzPb1857 JW5831 High-affinity zinc uptake system protein znuAPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.573SpI2318.3411-00Yn6-17c26/27oPeriplasmic1084.91
FIMI_ECOLIP39264
fimI
b4315 JW5779 Fimbrin-like protein fimIFim operon (class γ1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Disulfide bridge (43 83) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY200.716SpI1916.0857-00Yn6-13c19/20oExtracellular9.7237.53
YTFQ_ECOLIP39325
ytfQ
b4227 JW4186 ABC transporter periplasmic-binding protein ytfQPeriplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY220.849SpI2121.6548-10Yn5-13c21/22oPeriplasmic1090.28
OPGB_ECOLIP39401
mdoB

opgB yjjO

opgB yjjOb4359 JW5794 Phosphoglycerol transferase I (EC 2.7.8.20) (Phosphatidylglycerol--membrane-oligosaccharide glycerophosphotransferase)Cell inner membrane (Probable) Disulfide bond-containing protein PMID: 25664544PMID: 15919996out/outIntegral Inner Membrane BBIM (predicted)Cell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSRPSECnoyesN210.198TMH05.71979-44Yn4-14c18/19o28-47i59-74o80-98i110-128oCytoplasmicMembrane107.34
RIR3_ECOLIP39452
nrdE
b2675 JW2650 Ribonucleoside-diphosphate reductase 2 subunit alpha (EC 1.17.4.1) (R1E protein) (Ribonucleotide reductase 2) Disulfide bridge (178 415) CytoplasmicAAN/ACytoplasmicyesyesN300.107CYT0-0.200913-00="0"iCytoplasmic9.977.62
YGIL_ECOLIP39834
ygiL
b3043 JW3011 Uncharacterized fimbrial-like protein ygiLPutative chaperone–usher fimbrial operon (class π); Unknown function. Disulfide bridge (41 80) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY240.867SpI2315.5994-00Yn7-18c23/24oExtracellular9.7292.29
OPGD_ECOLIP40120
mdoD

opgD ydcG yzzZ

opgD ydcG yzzZb1424 JW1420 Glucans biosynthesis protein DPeriplasmic (Experimental)TAT secretion system. PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)TAT,SECnoyesY330.683SpI3218.7932-00Yn14-25c32/33oPeriplasmic1061.48
YRAH_ECOLIP42913
yraH
b3142 JW3111 Uncharacterized fimbrial-like protein yraHPotential fimbrial yra operon (class γ1); Fimbrial Subunit. Disulfide bridge (38 78) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY230.673SpI2211.7644-00Yn7-17c22/23oExtracellular9.72
YRAJ_ECOLIP42915
yraJ
b3144 JW3113 Uncharacterized outer membrane usher protein yraJPotential fimbrial yra operon (class γ1); Usher. Disulfide bridge (815 837) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMnoyesN410.51SpI409.33083-00="0"iOuterMembrane106.52
YHCD_ECOLIP45420
yhcD
b3216 JW3183 Uncharacterized outer membrane usher protein yehBPotential fimbrial yhc operon (class β); Uknown function. Disulfide bridge (769 792) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMnoyesY220.809SpI2110.0887-00Yn5-16c21/22oOuterMembrane10
LPOA_ECOLIP45464
lpoA

yraM

yraMb3147 JW3116 Uncharacterized protein yraMPeriplasm (Probable) Cell outer membrane (Probable) Outer membrane protein (Experimental)The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 21183073 PMID: 21183074 PMID: 20932056Outer Membrane LipoproteinIISoluble IM FractionMembrane LipoproteinSECLOLnoyesY260.162SpII2612.6937Pos+2=G00Yn14-27c45/46oCytoplasmicMembrane9.82105.05
AIS_ECOLIP45565
ais

pmrG

pmrGb2252 JW2246 Lipopolysaccharide core heptose(II)-phosphate phosphatase (EC 3.1.3.-) (Polymyxin resistance protein pmrG)Periplasmic protein by virtue of its homology with the well characterized Salmonella homologue PmrGPMID: 16803591. Disulfide bond-containing protein PMID: 25664544PMID: 16803591PeriplasmicGGN/APeriplasmMembrane anchoredSECyesyesN320.351SpI319.81523-10Yn16-27c31/32oUnknown2.550.08
LUXS_ECOLIP45578
luxS

ygaG

ygaGb2687 JW2662 S-ribosylhomocysteine lyase (EC 4.4.1.21) (AI-2 synthesis protein) (Autoinducer-2 production protein luxS)Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1N/ACytoplasmicTFnoyesN190.106CYT0-0.200913-00="0"iCytoplasmic8.96110.02
YHDW_ECOLIP45766
yhdW
b3268 JW3236 Putative amino-acid ABC transporter-binding protein yhdW Disulfide bond-containing protein PMID: 25664544PeriplasmicGGSoluble IM FractionCell inner membraneCytoplasmicSECpseudogene (Uniprot) nonoY250.628SpI2420.0416-00Yn6-17c24/25oPeriplasmic1089.71
YBHC_ECOLIP46130
ybhC
b0772 JW0755 Putative acyl-CoA thioester hydrolase ybhC (EC 3.1.2.-)Lipoprotein (Experimental) Outer membrane protein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) PMID: 10806384 Disulfide bridge (185 197) PMID: 19452549PMID: 15174130 PMID: 15911532 PMID: 10806384 PMID: 20932056Outer Membrane LipoproteinIISoluble IM FractionCell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLyesyesY240.274SpII2124.9976Pos+2=S00Yn9-19c24/25oOuterMembrane9.9371.31
DPAL_ECOLIP66899
ygeX
b2871 JW2839 Putative diaminopropionate ammonia-lyase (Diaminopropionatase) (EC 4.3.1.15) (Alpha,beta-diaminopropionate ammonia-lyase) Disulfide bridge (265 291) PMID: 22505717CytoplasmicAAN/ACytoplasmicyesyesN330.114CYT0-0.200913-00="0"iCytoplasmic9.2675.45
RCSF_ECOLIP69411
rcsF
b0196 JW0192 Protein rcsFOuter membrane Lipoprotein (Experimental) Cell outer membrane (Probable) Outer membrane protein (Experimental) Proven pignal peptidase II substrate. Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bridge (74 118;109 124) Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 16740933 PMID: 23813676 PMID: 20932056Outer Membrane LipoproteinIISoluble IM FractionCell outer membraneOuter membrane Lipoprotein (Experimental)SECDSBALOLyesyesY230.182SpII1513.2891Pos+2=S00Yn3-13c21/22oOuterMembrane1081.91
YAHK_ECOLIP75691
yahK
b0325 JW0317 Uncharacterized zinc-type alcohol dehydrogenase-like protein yahK (EC 1.-.-.-)Peripheral Inner membrane protein facing the cytoplasm (Potential) Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1IM (predicted-detected)CytoplasmicnoyesN530.148CYT0-0.200913-00="0"iCytoplasmic9.9773.5
YBGO_ECOLIP75748
ybgO
b0716 JW5098 Uncharacterized protein ybgOPutative chaperone–usher fimbrial operon yeh (class π); Unkown function. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicfimbriaeSECnoyesN240.359SpI237.76951-00Yn7-18c23/24oCytoplasmic8.9653.02
YBGQ_ECOLIP75750
ybgQ
b0718 JW5099 Uncharacterized outer membrane usher protein ybgQPutative chaperone–usher fimbrial operon yeh (class π); Usher. Disulfide bridge (795 814) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMnoyesY220.84SpI218.29107-00Yn6-16c21/22oOuterMembrane1010.92
FIU_ECOLIP75780
fiu

ybiL

ybiLb0805 JW0790 Catecholate siderophore receptor fiu (Ferric iron uptake protein) (TonB-dependent receptor fiu)Outer Membrane β-barrel protein (Experimental) Outer membrane protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 10806384 PMID: 20932056Outer Membrane b-barrel proteinHHN/ACell outer membraneOuter Membrane β-barrel protein (Experimental)SECSKPBAMyesyesY340.525SpI3311.8291-10Yn14-25c33/34oOuterMembrane1010.6
YBJP_ECOLIP75818
ybjP
b0865 JW0849 Uncharacterized lipoprotein ybjPLipoprotein (Experimental) Outer membrane protein (Experimental) Outer membrane Lipoprotein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Identified DsbA substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 10806384Outer Membrane LipoproteinIISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECDSBALOLnoyesY280.445SpII1810.6734Pos+2=T00Yn9-19c24/25oUnknown269.59
YDCS_ECOLIP76108
ydcS
b1440 JW1435 Putative ABC transporter periplasmic-binding protein ydcS Periplasmic (Experimental) (Experimental) Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY230.836SpI2217.8592-00Yn10-18c22/23oPeriplasmic1016.45
YNFB_ECOLIP76170
ynfB
b1583 JW1575 UPF0482 protein ynfBOuter membrane protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544PMID: 15911532Peripheral outer membrane protein facing the periplasmF3F3N/APeriplasmicSECyesyesY290.87SpI2819.5483-00Yn9-20c28/29oUnknown2.549.16
ZINT_ECOLIP76344
zinT

yodA

yodAb1973 JW1956 Metal-binding protein yodA (Cadmium-induced protein yodA)Cytoplasm, Periplasm (Probable) Periplasmic (Experimental)After 45 min of cadmium exposure the YodA protein was primarily detected in the cytoplasmic fraction but was later (150 min) found in both the cytoplasmic and periplasmic compartments. PMID: 9579078 Disulfide bond-containing protein PMID: 25664544PMID: 12480884 PMID: 9579078PeriplasmicGGN/APeriplasmic (Experimental)SECyesyesY240.683SpI2312.2997-00Yn8-18c23/24oUnknown2.5
YFCP_ECOLIP76499
yfcP
b2333 JW2330 Uncharacterized fimbrial-like protein yfcPPutative chaperone–usher fimbrial operon yfc (class π); Fimbrial Subunit. Disulfide bridge (43 83) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUyesyesY280.79SpI2714.214-10Yn8-19c27/28oUnknown2.5
YFEY_ECOLIP76537
yfeY
b2432 JW2425 Uncharacterized protein yfeYLipoprotein (Experimental) Outer membrane protein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIIN/AOuter membrane Lipoprotein (Experimental)SECLOLyesyesY290.248SpII1720.3796Pos+2=S00Yn8-19c27/28oUnknown259.44
YPFG_ECOLIP76559
ypfG
b2466 JW2450 Uncharacterized protein ypfG Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY220.804SpI2115.2807-10Yn5-16c21/22oCytoplasmicMembrane9.8661.8
YFHM_ECOLIP76578
yfhM
b2520 JW2504 Uncharacterized lipoprotein yfhM Identified DsbA substrate PMID: 19953543Inner Membrane LipoproteinEESoluble IM FractionCell membrane | Lipid-anchorInner membrane lipoproteinSECDSBALOLyesyesY250.18SpII1728.7232Pos+2=D00Yn6-13c18/19oUnknown4.69
YQIG_ECOLIP76655
yqiG
b3046 JW5507 Putative outer membrane usher protein yqiGPutative chaperone–usher fimbrial operon (class π); Usher. Disulfide bridge (798 817) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMnoyesN210.333CYT0-0.200913-00="0"iOuterMembrane10
DSBG_ECOLIP77202
dsbG

ybdP

ybdPb0604 JW0597 Thiol:disulfide interchange protein DsbGPeriplasmic (Experimental) Disulfide bridge (126 129) PMID: 15184683 Disulfide bond-containing protein PMID: 25664544PMID: 9383195PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY180.618SpI1712.1105-00Yn5-12c17/18oPeriplasmic1050.7
RCLA_ECOLIP77212
ykgC
b0304 JW5040 Probable pyridine nucleotide-disulfide oxidoreductase ykgC Disulfide bridge (43 48) CytoplasmicAAN/ACytoplasmicnoyesN200.197CYT0-0.200913-00="0"iCytoplasmic9.9746.88
YAGV_ECOLIP77263
yagV
b0289 JW5030 Uncharacterized protein yagVPutative chaperone–usher fimbrial operon yag(class α); Unknown function. Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicfimbriaeSECSURAnoyesY280.534SpI278.52618-10Yn12-22c27/28oUnknown2.517.86
YPHF_ECOLIP77269
yphF
b2548 JW2532 ABC transporter periplasmic-binding protein yphFPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY270.817SpI2620.8859-00Yn11-22c26/27oPeriplasmic9.7663.54
YDER_ECOLIP77294
ydeR
b1503 JW1497 Uncharacterized fimbrial-like protein ydeRPotential fimbrial yde operon (class γ1); Fimbrial Subunit. Disulfide bridge (39 77) PeriplasmicGGN/AFimbriumPeriplasmicfimbriaeSECCUnoyesY240.701SpI2320.0135-00Yn8-18c23/24oUnknown2.567.33
BORD_ECOLIP77330
borD

ybcU

ybcUb0557 JW0546 Lipoprotein bor homolog from lambdoid prophage DLP12Lipoprotein (Experimental)Proven pignal peptidase II substrate. PMID: 15174130 Disulfide bond-containing protein PMID: 25664544PMID: 15174130Outer Membrane LipoproteinIISoluble IM FractionCell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)SECLOLProphage Integrase (EcoGene)nonoY210.303SpII1621.3732Pos+2=A00Yn5-14c18/19oUnknown2.532.74
HLYE_ECOLIP77335
hlyE

clyA hpr sheA ycgD

clyA hpr sheA ycgDb1182 JW5181 Hemolysin E, chromosomal (Cytotoxin ClyA) (Hemolysis-inducing protein) (Latent pore-forming 34 kDa hemolysin) (Silent hemolysin SheA)Inner membrane (Potential) Periplasm, Extracellular space (Probable) Secreted and released (Experimental)HlyE(ClyA) forms a dodecameric transmembrane pore. The ability of ClyA to translocate to the periplasm is abolished in deletion mutants lacking the last 23 or 11 amino acid residues of the C-terminal region. Also, analysis of ClyA mutants with defined deletions and amino acid exchanges revealed that several ClyA segments which are distant of each other in the primary structure, namely, the N-terminal αA1 region, the C-terminal region, and the hydrophobic sequence ranging from residues 89 to 101, are crucial for ClyA translocation from the E. coli cytoplasm to the periplasm. In the soluble ClyA monomer, these regions are found closely adjacent to each other in the tail domain. ClyA assembles into an oligomeric structure in solution in the absence of either bilayer membranes or detergents at physiological temperature.These oligomers can rearrange to create transmembrane pores when in contact with detergents or biological membranes. ClyA does not form transmembrane pores on E. coli lipid membranes. Recombinant E. coli overexpressing the cloned clyA gene accumulated this haemolysin in the periplasm and released only very small amounts of it into the external medium. PMID: 11902713 Disulfide bridge (87 285) PMID: 14532000PMID: 20511497 PMID: 10027972 PMID: 11902713Periplasmic, Extra-cellularG, XG,XN/ASecretedPeriplasmHost cell membrane | Single-pass membrane proteinSecreted and released (Experimental)Uknown mechanismOMVnoyesN580.117CYT0-0.200913-10="0"iExtracellular1062.94
YBCL_ECOLIP77368
ybcL
b0545 JW0533 UPF0098 protein ybcLPeriplasm (Probable) Disulfide bridge (46 129) Disulfide bond-containing protein PMID: 25664544PMID: 11439028PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECProphage Integrase (EcoGene)yesnoY200.717SpI1919.8157-00Yn4-15c19/20oPeriplasmic1048.6
RCLR_ECOLIP77379
ykgD
b0305 JW0298 Uncharacterized HTH-type transcriptional regulator ykgDNucleoid (Experimental) Disulfide bridge (21 89) PMID: 24078635PMID: 23138451NucleoidNNIM (predicted)CytoplasmicnoyesN340.112CYT0-0.200913-00="0"iUnknown213.56
SFMD_ECOLIP77468
sfmD
b0532 JW0521 Outer membrane usher protein sfmDPotential fimbrial sfm operon (class γ1); Usher. Disulfide bridge (840 862) Outer Membrane b-barrel proteinHHN/ACell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel proteinfimbriaeSECBAMyesyesN360.404TMH03.9822-11="0"i12-32oOuterMembrane10
YAIW_ECOLIP77562
yaiW
b0378 JW0369 Uncharacterized protein yaiWOuter membrane protein (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 20932056Outer Membrane LipoproteinIIN/AOuter membrane LipoproteinSECLOLnoyesY260.354SpII2028.9818Pos+2=S00Yn7-18c23/24oUnknown2.510.13
THII_ECOLIP77718
thiI

yajK

yajKb0423 JW0413 tRNA sulfurtransferase (EC 2.8.1.4) (Sulfur carrier protein ThiS sulfurtransferase) (Thiamine biosynthesis protein thiI) (tRNA 4-thiouridine synthase) Disulfide bridge (344 456) PMID: 16855700CytoplasmicAAN/ACytoplasmCytoplasmicnoyesN550.111CYT0-0.200913-00="0"iCytoplasmic10
YDES_ECOLIP77789
ydeS
b1504 JW1498 Uncharacterized fimbrial-like protein ydeSPotential fimbrial yde operon (class γ1); Fimbrial Subunit. Disulfide bridge (38 78) Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumMembrane anchoredfimbriaeSECCUnoyesY230.426SpI2211.5353-10Yn6-17c22/23oExtracellular1079.19
COPA_ECOLIQ59385
copA

ybaR

ybaRb0484 JW0473 Copper-exporting P-type ATPase A (EC 3.6.3.n1)Integral inner membrane (Experimental) Integral Membrane Protein (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 10639134 Disulfide bond-containing protein PMID: 25664544PMID: 15919996 PMID: 10639134in/inIntegral Inner Membrane BBIM (predicted), Detected in IM FractionCell membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPYidCnoyesN500.162CYT0-0.200913-88="0"i185-205o217-238i245-264o284-302i436-458o464-487i778-797o803-824iCytoplasmicMembrane1021.12