entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refsnterm_ctermsuboci_fullsublocisubloci_iimbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
RIR1_ECOLIP00452
nrdA

dnaF

dnaFb2234 JW2228 cytoplasmic peripheral IM protein, Ribonucleoside-diphosphate reductase 1 subunit alpha NrdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490PMID: 23230279 PMID: 9395490Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicTFyesyesN400.104CYT0-0.200913-00NiCytoplasmic963
AK2H_ECOLIP00562
metL

metM

metMb3940 JW3911 cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 2 MetLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicyesyesN240.117CYT0-0.200913-00NiCytoplasmicMembrane9
PPB_ECOLIP00634
phoA
b0383 JW0374 periplasmic protein (SEC through IM), Alkaline phosphatase PhoAPeriplasmic (Probable) Periplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544PMID: 3533724 PMID: 7022451 PMID: 3009404 PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAnoyesY220.408SpI213.05238-00Yn5-16c21/22oPeriplasmic1078
FLHD_ECOLIP0A8S9
flhD

flbB

flbBb1892 JW1881 nucleoid protein, transcriptional regulator FlhDCytoplasmic, Nucleoid (Experimental) Disulfide bridge (65 65) PMID: 23138451Cytoplasmic, NucleoidA, NA,NN/ACytoplasmCytoplasmicnoyesN220.156CYT0-0.200913-00NiCytoplasmic988
YEDF_ECOLIP0AA31
yedF
b1930 JW1915 cytoplasmic protein, Putative sulfur carrier YedF Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN240.136CYT0-0.200913-00NiUnknown290
YAJD_ECOLIP0AAQ2
yajD
b0410 JW0400 cytoplasmic protein, Putative HNH nuclease YajD Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN380.105CYT0-0.200913-00NiUnknown2108
LEP_ECOLIP00803
lepB
b2568 JW2552 integral IM protein (SEC-YidC in IM), Signal peptidase I LepBIntegral Inner Membrane (Probable) Integral Inner Membrane (Experimental)Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased. Lep was shown to be targeted to the membrane by the SRP pathway. PMID: 8985168 Disulfide bridge (171 177) PMID: 21778229 PMID: 15919996 PMID: 8985168 out/outIntegral Inner MembraneBBIM (predicted)Cell inner membrane Integral Membrane ProteinSEC-YidCyesyesN220.131SpII203.04951Pos+2=V20Yn5-16c21/22oCytoplasmicMembrane1027
ASPG2_ECOLIP00805
ansB
b2957 JW2924 periplasmic protein (SEC through IM), L-asparaginase 2 AnsBPeriplasmic (Experimental)PMID: 387570 PMID: 2407723 PMID: 25403562 PMID: 8434007PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.82SpI2219.7315-10Yn7-17c22/23oPeriplasmic1049
SYI_ECOLIP00956
ileS

ilvS

ilvSb0026 JW0024 cytoplasmic protein, Isoleucine--tRNA ligase IleSTranslation unit. Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN610.113CYT0-0.200913-00NiCytoplasmic10
SYGA_ECOLIP00960
glyQ

glyS(A)

glyS(A)b3560 JW3531 cytoplasmic peripheral IM protein, Glycine--tRNA ligase alpha subunit GlyQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmCytoplasmicTFnoyesN570.112CYT0-0.200913-00NiCytoplasmic959
YBEL_ECOLIP0AAT9
ybeL
b0643 JW0638 cytoplasmic protein, YbeL Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN220.103CYT0-0.200913-00NiCytoplasmic895
YBGS_ECOLIP0AAV6
ybgS
b0753 JW0736 periplasmic protein (SEC through IM), YbgS Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY250.845SpI2432.7676-00Yn6-17c24/25oPeriplasmic9
YCAR_ECOLIP0AAZ7
ycaR
b0917 JW0900 cytoplasmic protein, UPF0434 YcaR Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN560.117CYT0-0.200913-00NiUnknown2
LAMB_ECOLIP02943
lamB

malB

malBb4036 JW3996 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Maltoporin LamBOuter Membrane b-barrel protein (Probable) Outer Membrane b-barrel protein (Experimental)Potential pubstrate of periplasmic chaperones Skp. Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000). Experimentally verified trimeric beta-barrel. N-terminal region is in periplasm. Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 346375 Disulfide bridges between 47 and 63 PMID: 1988451 Disulfide bond-containing protein PMID: 25664544PMID: 16372265 PMID: 15911532 PMID: 10806384 PMID: 16079137 PMID: 20932056 PMID: 346375 PMID: 1988451Outer Membrane b-barrel proteinHHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)SECSKP,SURA,DEGPBAMyesyesY260.795SpI2520.7922-00Yn8-18c25/26oOuterMembrane1054
FIMA1_ECOLIP04128
fimA

pilA

pilAb4314 JW4277 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), Type-1 protein, A chain FimAPeripheral outer membrane protein facing the extra-cellular space (Experimental)Fim operon (class ??1); Fimbrial Subunit. Experimentally verified signal peptide PMID: 2858471 Disulfide bridge (44 84) Disulfide bond-containing protein PMID: 25664544PMID: 2858471Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUyesyesY240.78SpI2325.7568-10Yn5-16c23/24oExtracellular10
TYRB_ECOLIP04693
tyrB
b4054 JW4014 cytoplasmic protein, Aromatic-amino-acid aminotransferase TyrB Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN100.122CYT0-0.200913-00NiCytoplasmic1038
LIVK_ECOLIP04816
livK
b3458 JW3423 periplasmic protein (SEC through IM), Leucine-specific-binding LivKPeriplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 22030350 PMID: 8061609PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.817SpI2317.7516-00Yn7-18c23/24oPeriplasmic1078
YMGD_ECOLIP0AB46
ymgD
b1171 JW5177 periplasmic protein (SEC through IM), YmgD Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECnoyesY200.656SpI1914.7848-00Yn4-14c19/20oUnknown286
ACYP_ECOLIP0AB65
yccX
b0968 JW5131 cytoplasmic protein, Acylphosphatase YccX Disulfide bridge (5 49) PMID: 17134700CytoplasmicAAN/ACytoplasmicnoyesN280.119CYT0-0.200913-00Yn4-11c16/17oCytoplasmic9103
FEPA_ECOLIP05825
fepA

fep feuB

fep feuBb0584 JW5086 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrienterobactin receptor FepAOuter Membrane b-barrel protein (Probable) Outer Membrane b-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544PMID: 11266632 PMID: 9886293 PMID: 20932056 PMID: 10806384 PMID: 10529396Outer Membrane b-barrel proteinHHN/ACell outer membrane; Multi-pass membrane proteinOuter Membrane ??-barrel protein (Experimental)SECSKPBAMyesyesY230.836SpI2211.5401-00Yn7-17c22/23oOuterMembrane1011
BTUE_ECOLIP06610
btuE
b1710 JW1700 cytoplasmic protein, Thioredoxin/glutathione peroxidase BtuE Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/APeriplasm CytoplasmicyesyesN370.111CYT0-0.200913-00NiPeriplasmic1099
GSHR_ECOLIP06715
gor
b3500 JW3467 cytoplasmic protein, Glutathione reductase GorCytoplasmic (Experimental) Disulfide bridge (42 47) PMID: 8061609PMID: 8061609CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN240.239CYT0-0.200913-00NiCytoplasmic1067
FHUA_ECOLIP06971
fhuA

tonA

tonAb0150 JW0146 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrichrome transporter/phage receptor FhuAOuter Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable)SecB substrate; Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 3079747 Disulfide bridge (351 362;725 731) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 10806384 PMID: 11266632 PMID: 9856937 PMID: 3079747Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)SECBSECSURA,SKPBAMyesyesY340.805SpI3320.4987-00Yn16-24c33/34oOuterMembrane107
NRDH_ECOLIP0AC65
nrdH

ygaN

ygaNb2673 JW2648 cytoplasmic protein, Glutaredoxin-like NrdH NrdH Disulfide bridge (11 14) CytoplasmicAAN/ACytoplasmicnoyesN570.105CYT0-0.200913-00NiUnknown2102
YDCA_ECOLIP0ACW4
ydcA
b1419 JW1416 periplasmic protein (SEC through IM), YdcA Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECnoyesY210.761SpI2012.9463-00Yn4-15c20/21oUnknown2
USHA_ECOLIP07024
ushA
b0480 JW0469 periplasmic protein (SEC through IM), UshAPeriplasmic (Experimental)PMID: 9298646PeriplasmicGGSoluble IM FractionPeriplasm Periplasmic (Experimental)SECnoyesY260.748SpI2511.3752-00Yn7-18c25/26oPeriplasmic1077
PPA_ECOLIP07102
appA
b0980 JW0963 periplasmic protein (SEC through IM), AppA Periplasmic (Experimental) Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544PMID: 8387749PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBCyesyesY230.793SpI2213.6965-00Yn3-13c22/23oPeriplasmic1066
TDH_ECOLIP07913
tdh
b3616 JW3591 cytoplasmic protein, L-threonine 3-dehydrogenase Tdh Disulfide bond-containing protein PMID: 25664544CytoplasmicAAIM (predicted)Cytoplasm CytoplasmicyesyesN250.103CYT0-0.200913-00NiCytoplasmic1050
ILVB_ECOLIP08142
ilvB
b3671 JW3646 cytoplasmic protein, Acetolactate synthase isozyme 1 large subunit IlvB Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN450.118CYT0-0.200913-00NiCytoplasmic929
YHEV_ECOLIP0ADW8
yheV
b4551 JW3312 cytoplasmic protein, YheV Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN240.14CYT0-0.200913-00NiUnknown2
BCP_ECOLIP0AE52
bcp
b2480 JW2465 cytoplasmic protein, Peroxiredoxin Bcp Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN510.123CYT0-0.200913-00NiCytoplasmic8
CREA_ECOLIP0AE91
creA

yjjD

yjjDb4397 JW4360 periplasmic protein (SEC through IM), CreA Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECDSBAyesyesY220.8SpI2114.9398-00Yn6-16c21/22oUnknown2
FIMF_ECOLIP08189
fimF
b4318 JW4281 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimFPeripheral outer membrane protein facing the extra-cellular space (Experimental)Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (38 78) Disulfide bond-containing protein PMID: 25664544PMID: 15372038Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY230.871SpI2217.2366-00Yn6-17c22/23oExtracellular9
FIMG_ECOLIP08190
fimG
b4319 JW4282 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimGPeripheral outer membrane protein facing the extra-cellular space (Experimental)Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (39 77) Disulfide bond-containing protein PMID: 25664544PMID: 15372038Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbriumCell surface appendage (Experimental)fimbriaeSECCUnoyesY240.773SpI2318.1281-10Yn7-18c23/24oExtracellular973
FOLC_ECOLIP08192
folC

dedC

dedCb2315 JW2312 cytoplasmic protein, Dihydrofolate synthase/folylpolyglutamate synthase FolC Disulfide bridge (354 401) CytoplasmicAAN/ACytoplasmicyesyesN260.113CYT0-0.200913-00NiCytoplasmic984
CPDB_ECOLIP08331
cpdB
b4213 JW4171 periplasmic protein (SEC through IM), 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase CpdBPeriplasmic (Probable) Periplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 3005231 PMID: 15911532 PMID: 9298646PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECSURAnoyesY200.787SpI1914.5002-00Yn4-14c19/20oPeriplasmic1082
PSIF_ECOLIP0AFM4
psiF
b0384 JW5054 periplasmic protein (SEC through IM), Phosphate starvation-inducible PsiF Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY220.376SpI2113.709-10Yn5-16c21/22oUnknown244
YJJA_ECOLIP18390
yjjA
b4360 JW5795 periplasmic protein (SEC through IM), YjjA Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY260.86SpI2523.6068-00Yn8-19c25/26oUnknown2100
YEBY_ECOLIP64506
yebY
b1839 JW1828 periplasmic protein (SEC through IM), YebY Identified DsbA substrate PMID: 19953543PeriplasmicGGN/APeriplasmicSECDSBAyesyesY210.862SpI2019.6801-10Yn5-12c20/21oUnknown235
AK3_ECOLIP08660
lysC

apk

apkb4024 JW3984 cytoplasmic protein, Lysine-sensitive aspartokinase 3 LysC Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN430.206CYT0-0.200913-00NiUnknown4
PT1_ECOLIP08839
ptsI
b2416 JW2409 cytoplasmic peripheral IM protein, Phosphoenolpyruvate-phosphotransferase PtsICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 2644649 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasm CytoplasmicyesyesN150.115CYT0-0.200913-00NiCytoplasmic1090
ARGT_ECOLIP09551
argT
b2310 JW2307 periplasmic protein (SEC through IM), Lysine/arginine/ornithine-binding periplasmic ArgTPeriplasmic (Experimental)This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 9298647 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY230.595SpI2219.5206-00Yn4-14c22/23oPeriplasmic10
GLTB_ECOLIP09831
gltB

aspB

aspBb3212 JW3179 cytoplasmic peripheral IM protein, Glutamate synthase (NADPH) large chain GltBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicnoyesN480.135CYT0-0.200913-00NiCytoplasmic9
YFIR_ECOLIP64548
yfiR
b2603 JW2584 periplasmic protein (SEC through IM), YfiR Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasm PeriplasmicSECyesyesY230.684SpI2213.4131-00Yn7-17c22/23oUnknown621
LEUC_ECOLIP0A6A6
leuC
b0072 JW0071 cytoplasmic protein, 3-isopropylmalate dehydratase large subunit LeuC Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN410.123CYT0-0.200913-00NiCytoplasmic970
DAPF_ECOLIP0A6K1
dapF
b3809 JW5592 cytoplasmic protein, Diaminopimelate epimerase DapF Disulfide bridge (73 217) CytoplasmicAAIM (predicted)Cytoplasm CytoplasmicyesyesN190.106CYT0-0.200913-00NiCytoplasmic917
DSBB_ECOLIP0A6M2
dsbB

roxB ycgA

roxB ycgAb1185 JW5182 integral IM protein (SEC-YidC in IM), Disulfide bond formation B DsbBIntegral Inner Membrane (Experimental) Integral Inner Membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC PMID: 22108384. The crystal structure revealed a transmembrane (TM) four-helix-bundle structure of DsbBPMID: 18406599. DsbB oxidizes the periplasmic protein DsbA, which in turn oxidizes the cysteines in other periplasmic proteins to make disulfide bondsPMID: 8430071. Gene fusion approaches were used to show that (i) DsbB is a membrane protein which spans the membrane four times and (ii) both the N- and C-termini of the protein are in the cytoplasmPMID: 7957076. The structure of DsbB revealed four transmembrane helices and one short horizontal helix juxtaposed with Cys130 in the mobile periplasmic loopPMID: 17110337. Disulfide bridge (41 44;104 130) PMID: 10064586PMID: 18406599 PMID: 17110337 PMID: 8430071 PMID: 7957076 PMID: 15919996in/inIntegral Inner MembraneBBIM (predicted), Detected in IM FractionCell inner membrane Integral Membrane ProteinSRPSEC-YidCyesyesN250.286TMH04.8983-44Ni12-33o45-64i71-89o145-166iCytoplasmicMembrane1015
FLGI_ECOLIP0A6S3
flgI

fla FIX flaM

fla FIX flaMb1080 JW1067 Flagellar periplasmic protein (SEC through IM), P-ring FlgIPeriplasmic (Experimental)P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 12730325 PMID: 15838024PeriplasmicGGN/APeriplasm Bacterial flagellum basal bodyPeriplasmic (Experimental)flagellumSRP (exp)SECDSBAFlanoyesY200.783SpI1915.6262-00Yn4-14c19/20oPeriplasmic1063
GLRX1_ECOLIP68688
grxA

grx

grxb0849 JW0833 cytoplasmic protein, Glutaredoxin 1 GrxA Disulfide bridge (11 14) CytoplasmicAAN/ACytoplasmicyesyesN180.204CYT0-0.200913-00NiUnknown2
YCGJ_ECOLIP76001
ycgJ
b1177 JW1166 periplasmic protein (SEC through IM), YcgJ Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY230.404SpI228.58297-00Yn6-16c24/25oUnknown277
YNJH_ECOLIP76227
ynjH
b1760 JW1749 periplasmic protein (SEC through IM), YnjH Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECnoyesY190.569SpI1811.8884-00Yn4-14c18/19oCytoplasmicMembrane927
GSH1_ECOLIP0A6W9
gshA

gsh-I

gsh-Ib2688 JW2663 cytoplasmic protein, Glutamate--cysteine ligase GshA Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN210.149CYT0-0.200913-00NiCytoplasmic926
HSLO_ECOLIP0A6Y5
hslO

yrfI

yrfIb3401 JW5692 cytoplasmic peripheral IM protein, 33 kDa chaperonin HslOCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (230 232;263 266) PMID: 10764757PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1IM (predicted)CytoplasmCytoplasmicyesyesN630.125CYT0-0.200913-00NiCytoplasmic1088
MSRA_ECOLIP0A744
msrA

pms

pmsb4219 JW4178 cytoplasmic protein, Peptide methionine sulfoxide reductase MsrA Cytoplasmic (Experimental) YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300 Disulfide bridge (52 199); (199 207) PMID: 17272300CytoplasmicAAN/ACytoplasmicyesyesN160.11CYT0-0.200913-00NiUnknown285
MSRB_ECOLIP0A746
msrB

yeaA

yeaAb1778 JW1767 cytoplasmic protein, Peptide methionine sulfoxide reductase MsrB Cytoplasmic (Experimental)Fluorescent fusions of YeaA have been monitored via fluorescence microscopy. YeaA was found to be located evenly throughout cytoplasm. Disulfide bond-containing protein PMID: 25664544PMID: 17272300CytoplasmicAAN/ACytoplasmicyesyesN620.122CYT0-0.200913-00NiCytoplasmic996
NAGB_ECOLIP0A759
nagB

glmD

glmDb0678 JW0664 cytoplasmic peripheral IM protein, Glucosamine-6-phosphate deaminase NagBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (219 219) PMID: 8240271PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1IM (predicted)CytoplasmicyesyesN320.107CYT0-0.200913-00NiCytoplasmic828
MSRC_ECOLIP76270
msrC

yebR

yebRb1832 JW1821 cytoplasmic protein, Free methionine-R-sulfoxide reductase MsrC Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicnoyesN200.13CYT0-0.200913-00NiUnknown257
FLHE_ECOLIP76297
flhE
b1878 JW1867 periplasmic protein (SEC through IM), FlhEPart of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECFlayesyesY170.511SpI168.24229-00Yn3-14c26/27oUnknown268
YKFB_ECOLIP77162
ykfB
b0250 JW0239 periplasmic protein (SEC through IM), YkfB Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECProphage Integrase (EcoGene)nonoY240.716SpI2317.8556-00Yn11-19c23/24oUnknown259
PYRI_ECOLIP0A7F3
pyrI
b4244 JW4203 cytoplasmic protein, Aspartate carbamoyltransferase regulatory chain PyrICytoplasmic (Experimental)PMID: 3912513 PMID: 8740179CytoplasmicAAN/ACytoplasmic (Experimental)yesyesN370.104CYT0-0.200913-00NiUnknown2116
RECA_ECOLIP0A7G6
recA

lexB recH rnmB tif umuB zab

lexB recH rnmB tif umuB zabb2699 JW2669 cytoplasmic peripheral IM protein, RecACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 2228969Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCytoplasmCytoplasmicTFnoyesN30.103CYT0-0.200913-00NiCytoplasmic1088
RL31_ECOLIP0A7M9
rpmE
b3936 JW3907 ribosomal protein, 50S L31 RpmECytoplasmic, Ribosomal (Probable) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544PMID: 21541338Cytoplasmic, RibosomalA, rA,rN/ACytoplasmicribosomeyesyesN310.105CYT0-0.200913-00NiCytoplasmic966
RS12_ECOLIP0A7S3
rpsL

strA

strAb3342 JW3304 ribosomal protein, 30S S12 RpsLCytoplasmic, Ribosomal (Probable) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544PMID: 21541338Cytoplasmic, RibosomalA, rA,rSoluble IM FractionCytoplasmicribosomenoyesN270.105CYT0-0.200913-00NiCytoplasmic1084
TPX_ECOLIP0A862
tpx

yzzJ

yzzJb1324 JW1317 cytoplasmic peripheral IM protein, Thiol peroxidase TpxCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. PMID: 7499381 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 7499381 PMID: 9298646Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/APeriplasm Periplasmic (Experimental)yesyesN330.123CYT0-0.200913-00NiPeriplasmic10
RL362_ECOLIQ2EEQ2
ykgO

rpmJ2

rpmJ2b4506 JW5034 ribosomal protein, 50S L36 2 YkgOTranslation, Ribosomal. Expressed during stationary phase in minimal medium (at protein level). Disulfide bond-containing protein PMID: 25664544Cytoplasmic, RibosomalA, rA,rN/A-ribosomenoyesN380.12CYT0-0.200913-00NiUnknown2136
YICS_ECOLIQ2M7X4
yicS
b4555 JW5965 periplasmic protein (SEC through IM), YicS Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesN220.681SpI2110.9898-00Yn4-14c21/22oUnknown2
OMPA_ECOLIP0A910
ompA

con tolG tut

con tolG tutb0957 JW0940 OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpAOuter Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable) Outer Membrane b-barrel protein (Potential)Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544PMID: 10806384 PMID: 9808047 PMID: 7813480 PMID: 16079137 PMID: 21778229 PMID: 20932056 PMID: 19953543 PMID: 9298646 PMID: 24717922Outer Membrane b-barrel proteinHHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)TFSECSKP,SURA,DEGP,DSBABAMyesyesY220.872SpI2128.1613-00Yn5-16c21/22oOuterMembrane1010
ALF1_ECOLIP0A991
fbaB

dhnA

dhnAb2097 JW5344 cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 1 FbaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasm CytoplasmicnoyesN640.115CYT0-0.200913-00NiUnknown24
FUR_ECOLIP0A9A9
fur
b0683 JW0669 nucleoid protein, Ferric uptake regulation FurCytoplasmic, Nucleoid (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23138451Cytoplasmic, NucleoidA, NA,NN/ACytoplasmCytoplasmicyesyesN370.121CYT0-0.200913-00NiCytoplasmic10109
G3P1_ECOLIP0A9B2
gapA
b1779 JW1768 cytoplasmic peripheral IM protein, Glyceraldehyde-3-phosphate dehydrogenase A GapACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCytoplasm CytoplasmicTFyesyesN210.121CYT0-0.200913-00NiCytoplasmic973
ACEA_ECOLIP0A9G6
aceA

icl

iclb4015 JW3975 cytoplasmic peripheral IM protein, Isocitrate lyase AceACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicyesyesN300.126CYT0-0.200913-00NiCytoplasmic1042
DLDH_ECOLIP0A9P0
lpdA

lpd

lpdb0116 JW0112 cytoplasmic peripheral IM protein, Dihydroyl dehydrogenase LpdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 6352260 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (45 50) Disulfide bond-containing protein PMID: 25664544PMID: 16079137 PMID: 6352260 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)TFyesyesN230.176CYT0-0.200913-00NiCytoplasmic1079
TRXB_ECOLIP0A9P4
trxB
b0888 JW0871 cytoplasmic protein, Thioredoxin reductase TrxB Disulfide bridge (136 139) PMID: 8114095CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN260.317SpI256.69011-00Yn9-17c25/26oCytoplasmic10104
THIO_ECOLIP0AA25
trxA

fipA tsnC

fipA tsnCb3781 JW5856 cytoplasmic peripheral IM protein, Thioredoxin 1 TrxACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bridge (33 36) PMID: 10489448 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10489448Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicTFnoyesN200.119CYT0-0.200913-00NiCytoplasmic989
DSBE_ECOLIP0AA86
dsbE

ccmG yejQ

ccmG yejQb2195 JW2183 integral IM protein (SEC in IM), Thiol:disulfide interchange DsbE Integral Inner Membrane (Experimental)PMID: 9537397 PMID: 16364305in/outIntegral Inner MembraneBBN/ACell inner membrane; Single-pass membrane protein; Periplasmic sidePeriplasmic with N-terminal membrane anchor (Experimental)SRPSECyesyesN290.264SpI278.59712-10Yn6-21c30/31oCytoplasmicMembrane1069
EFEO_ECOLIP0AB24
efeO

ycdO

ycdOb1018 JW1003 periplasmic protein (SEC through IM), Iron uptake system component EfeO Periplasmic (Probable) Periplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 16551627 PMID: 12107143 PMID: 22030350PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECDSBAyesyesY270.767SpI2618.2235-00Yn9-18c26/27oPeriplasmic1042
CDD_ECOLIP0ABF6
cdd
b2143 JW2131 cytoplasmic protein, Cytidine deaminase CddOne very weak transmembrane reg ion pred ict ion. Disulfide bond-containing protein PMID: 25664544CytoplasmicAAIM (predicted)CytoplasmicyesyesN260.113CYT0-0.200913-00NiUnknown242
NRFA_ECOLIP0ABK9
nrfA
b4070 JW4031 periplasmic protein (SEC through IM), Cytochrome c-552 NrfAPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 793493PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY270.665SpI2614.744-00Yn11-21c26/27oPeriplasmic1025
NRFB_ECOLIP0ABL1
nrfB

yjcI

yjcIb4071 JW4032 periplasmic protein (SEC through IM), Cytochrome c-type NrfBPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 15280383PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECnoyesY330.498SpI256.62799-00Yn7-18c23/24oPeriplasmic916
NAPB_ECOLIP0ABL3
napB

yejY

yejYb2203 JW5367 periplasmic protein (SEC through IM), nitrate reductase, electron transfer subunit NapBPeriplasmic (Probable) Periplasmic (Experimental) Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 10548535 PMID: 17130127 PMID: 11844760PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY280.825SpI2714.6891-00Yn9-20c27/28oPeriplasmic10
SFMA_ECOLIP0ABW5
sfmA
b0530 JW0519 Fimbrial periplasmic protein (SEC through IM), SfmAPotential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (41 81) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUyesyesY230.832SpI2221.4596-00Yn6-17c22/23oExtracellular1083
FUMA_ECOLIP0AC33
fumA
b1612 JW1604 cytoplasmic peripheral IM protein, Fumarate hydratase class I, aerobic FumACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicyesyesN570.116CYT0-0.200913-00NiCytoplasmic937
GLRX2_ECOLIP0AC59
grxB
b1064 JW1051 cytoplasmic protein, Glutaredoxin 2 GrxB Disulfide bridge (9 12) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN330.104CYT0-0.200913-00NiCytoplasmic865
GLRX3_ECOLIP0AC62
grxC

yibM

yibMb3610 JW3585 cytoplasmic peripheral IM protein, Glutaredoxin 3 GrxCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (12 15) PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicyesyesN300.112CYT0-0.200913-00NiUnknown279
ISCU_ECOLIP0ACD4
iscU

nifU yfhN

nifU yfhNb2529 JW2513 cytoplasmic peripheral IM protein, Iron-sulfur cluster assembly scaffold IscU Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicyesyesN200.105CYT0-0.200913-00NiCytoplasmic997
MBHM_ECOLIP0ACE0
hybC
b2994 JW2962 periplasmic peripheral IM protein (TAT via piggyback through IM), Hydrogenase-2 large chain HybCPeripheral inner membrane protein facing the periplasm (Experimental)The protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system (piggyback). PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544PMID: 3516690 PMID: 8772179 PMID: 9738917Peripheral inner membrane protein facing the periplasmF2F2Soluble IM FractionCell membrane; Peripheral membrane proteinPeriplasmic (Experimental)TAT (piggyback)yesyesN340.139CYT0-0.200913-00NiCytoplasmicMembrane1028
OXYR_ECOLIP0ACQ4
oxyR

momR mor

momR morb3961 JW3933 nucleoid protein, Hydrogen peroxide-inducible genes activator OxyRCytoplasmic, Nucleoid (Experimental) Disulfide bridge (180 259) PMID: 12015987 Disulfide bridge (199 208) PMID: 9497290PMID: 23138451Cytoplasmic, NucleoidA, NA,NSoluble IM FractionCytoplasmicyesyesN220.192CYT0-0.200913-00NiCytoplasmic916
IVY_ECOLIP0AD59
ivy

ykfE

ykfEb0220 JW0210 periplasmic protein (SEC through IM), Inhibitor of vertebrate lysozyme IvyPeriplasmic (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544PMID: 11278658PeriplasmicGGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECyesyesY290.849SpI2816.6514-00Yn13-23c28/29oPeriplasmic1027
AMPH_ECOLIP0AD70
ampH

yaiH

yaiHb0376 JW5052 cytoplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein PMID: 25664544PMID: 22001512Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACell inner membrane PeriplasmicSURAyesyesY220.613SpI2116.801-00Yn5-16c21/22oPeriplasmic941
LIVJ_ECOLIP0AD96
livJ
b3460 JW3425 periplasmic protein (SEC through IM), Leu/Ile/Val-binding LivJPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 22030350PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.832SpI2320.6668-00Yn7-18c23/24oPeriplasmic1096
OSME_ECOLIP0ADB1
osmE

anr

anrb1739 JW1728 OM lipoprotein (SEC through IM), inserted in OM by LOL), Osmotically-inducible putative OsmE Outer Membrane Lipoprotein (Experimental)Proven signal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 10806384Outer Membrane LipoproteinIISoluble IM FractionCell inner membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY240.207SpII2020.0226Pos+2=T10Yn7-18c23/24oUnknown298
YGGN_ECOLIP0ADS9
yggN
b2958 JW2925 periplasmic protein (SEC through IM), YggNPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535PMID: 14726535PeriplasmicGGN/APeriplasmic(experimental)SECDSBAyesyesY210.797SpI2021.2471-00Yn6-16c20/21oUnknown280
YGIB_ECOLIP0ADT2
ygiB
b3037 JW5927 cytoplasmic peripheral IM protein, UPF0441 YgiBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein PMID: 25664544PMID: 20545840 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCytoplasmicyesyesN360.178SpII369.72422Pos+2=E00Yn21-32c37/38oPeriplasmic9
AHPC_ECOLIP0AE08
ahpC
b0605 JW0598 cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase C AhpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (47 47;166 166) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasm CytoplasmicyesyesN490.111CYT0-0.200913-00NiCytoplasmic1090
DSBA_ECOLIP0AEG4
dsbA

dsf ppfA

dsf ppfAb3860 JW3832 periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbA Periplasmic (Experimental)Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 15838024 PMID: 13129941PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SRP (exp)SECyesyesY200.75SpI1916.7987-00Yn4-15c19/20oPeriplasmic1071
DSBC_ECOLIP0AEG6
dsbC

xprA

xprAb2893 JW2861 periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbC Periplasmic (Probable) Periplasmic (Experimental)Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544PMID: 8168498 PMID: 9298646PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY210.778SpI2021.5074-00Yn4-15c20/21oPeriplasmic1082
HDEA_ECOLIP0AES9
hdeA

yhhC yhiB

yhhC yhiBb3510 JW3478 periplasmic protein (SEC through IM), Acid stress chaperone HdeAPeriplasmic (Probable) Periplasmic (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544PMID: 10623550 PMID: 17085547 PMID: 8455549 PMID: 25403562PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECYfgMyesyesY220.698SpI2116.385-10Yn4-15c21/22oUnknown2108
HDEB_ECOLIP0AET2
hdeB

yhhD yhiC

yhhD yhiCb3509 JW5669 periplasmic protein (SEC through IM), Acid stress chaperone HdeBPeriplasmic (Probable) Periplasmic (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 8455550 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bond-containing protein PMID: 25664544PMID: 17085547 PMID: 8455550 PMID: 25403562PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECYfgMyesyesY300.837SpI2916.8932-00Yn10-21c29/30oUnknown263
HDHA_ECOLIP0AET8
hdhA

hsdH

hsdHb1619 JW1611 cytoplasmic protein, 7-alpha-hydroxysteroid dehydrogenase HdhA Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN480.2CYT0-0.200913-00NiCytoplasmic968
HISJ_ECOLIP0AEU0
hisJ
b2309 JW2306 periplasmic protein (SEC through IM), Histidine-binding periplasmic HisJPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY230.792SpI2220.7158-10Yn4-14c22/23oPeriplasmic10
YJCO_ECOLIP0AF56
yjcO
b4078 JW4039 periplasmic protein (SEC through IM), Sel1-repeat-containing YjcO Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY180.752SpI1713.9637-00Yn4-13c17/18oUnknown282
PROX_ECOLIP0AFM2
proX

proU

proUb2679 JW2654 periplasmic protein (SEC through IM), Glycine betaine/proline betaine-binding periplasmic ProXPeriplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Identified DsbA substrate PMID: 19953543 PMID: 3305496 Disulfide bridge (157 163) Disulfide bond-containing protein PMID: 25664544PMID: 19953543 PMID: 3305496PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECSURA,DSBAyesyesY220.863SpI2116.9579-00Yn5-16c21/22oPeriplasmic1052
UGPB_ECOLIP0AG80
ugpB
b3453 JW3418 periplasmic protein (SEC through IM), sn-glycerol-3-phosphate-binding periplasmic UgpB Periplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 3062310 Disulfide bond-containing protein PMID: 25664544PMID: 3062310PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY240.804SpI2317.1867-00Yn7-18c23/24oPeriplasmic1064
SLT_ECOLIP0AGC3
slt

sltY

sltYb4392 JW4355 periplasmic protein (SEC through IM), Soluble lytic murein transglycosylase SltPeriplasmic (Experimental) Disulfide bridge (133 166) Disulfide bond-containing protein PMID: 25664544PMID: 1938883PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY280.803SpI2712.0915-00Yn11-22c27/28oPeriplasmic1010
SODC_ECOLIP0AGD1
sodC
b1646 JW1638 periplasmic protein (SEC through IM), Superoxide dismutase SodCPeriplasmic (Experimental) Disulfide bridge (74 169) PMID: 9405149 Disulfide bond-containing protein PMID: 25664544PMID: 8626323PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY200.82SpI1916.2903-00Yn4-14c19/20oPeriplasmic10
THIO2_ECOLIP0AGG4
trxC

yfiG

yfiGb2582 JW2566 cytoplasmic protein, Thioredoxin 2 TrxC Disulfide bridge (64 67) Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN700.102CYT0-0.200913-00NiCytoplasmic1092
MEPA_ECOLIP0C0T5
mepA
b2328 JW2325 periplasmic protein (SEC through IM), Penicillin-insensitive murein endopeptidase MepAPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2187143 Disulfide bridge (44 265;187 235;216 223) Disulfide bond-containing protein PMID: 25664544PMID: 2187143PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECDSBA,DSBCyesyesY200.847SpI1922.606-00Yn4-14c19/20oPeriplasmic1016
DEGP_ECOLIP0C0V0
degP

htrA ptd

htrA ptdb0161 JW0157 periplasmic protein (SEC through IM), serine endoprotease DegP Periplasmic (Probable) Periplasmic (Experimental)SecB substrate. Substrate of periplasmic disulfide oxidoreductases DsbA. HtrA was not detected by the immunoblotting technique in the periplasm or in the fraction of soluble proteins but was found in the inner membrane. DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into 12 and 24-mer cages with large chambers that house the substrate for degradation or refolding. Size exclusion chromatography and electron microscopy show that the size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme. Identified DsbA substrate PMID: 19953543 PMID: 2180903 Disulfide bridges between 83 and 95 PMID: 12878036 Disulfide bridge (83 95) PMID: 12878036 Disulfide bond-containing protein PMID: 25664544PMID: 9083020 PMID: 2180903 PMID: 12878036PeriplasmicGGN/ACell inner membrane Periplasmic (Experimental)SECBSECDSBAyesyesY270.841SpI2628.3128-00Yn4-19c26/27oPeriplasmic1031
GATZ_ECOLIP0C8J8
gatZ
b2095 JW2082 cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCytoplasmicTFyesyesN330.318CYT0-0.200913-00NiCytoplasmic86
EFTU1_ECOLIP0CE47
tufA
b3339 JW3301 cytoplasmic peripheral IM protein, Elongation factor Tu 1 TufACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. PMID: 7525272 PMID: 7021545 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451 Disulfide bond-containing protein PMID: 25664544PMID: 20133608 PMID: 2022614 PMID: 775340 PMID: 7525272 PMID: 7021545 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasm Cell inner membrane; Peripheral membrane protein Cytoplasmic (Experimental)yesyesN470.197CYT0-0.200913-00NiCytoplasmic1099
NADA_ECOLIP11458
nadA

nicA

nicAb0750 JW0733 cytoplasmic protein, Quinolinate synthase A NadA Disulfide bridge (291 294) CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN580.105CYT0-0.200913-00NiCytoplasmic1034
TREA_ECOLIP13482
treA

osmA

osmAb1197 JW1186 periplasmic protein (SEC through IM), trehalase TreAPeriplasmic (Experimental) Potential Skp substrate PMID: 19953543 PMID: 2671658 Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PMID: 2671658PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECSKPyesyesY310.702SpI3017.5054-00Yn13-26c30/31oPeriplasmic1070
CHIA_ECOLIP13656
chiA

yheB

yheBb3338 JW3300 periplasmic & extra-cellular protein (SEC through IM), TSS secreted over OM), Probable bifunctional chitinase/lysozyme ChiAPeriplasmic, Extra-cellular (Probable)Can be fully secreted when the cryptic T2S system is expressed. PMID: 10760150 Disulfide bridge (628 673) Disulfide bond-containing protein PMID: 25664544PMID: 11118204 PMID: 10760150Periplasmic, Extra-cellularG, XG,XN/APeriplasm Periplasmic (Experimental)SECT2SnoyesY250.672SpI2420.7505-00Yn11-20c24/25oPeriplasmic10
FUMB_ECOLIP14407
fumB
b4122 JW4083 cytoplasmic protein, Fumarate hydratase class I, anaerobic FumB Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN320.117CYT0-0.200913-00NiCytoplasmic969
SELD_ECOLIP16456
selD

fdhB

fdhBb1764 JW1753 cytoplasmic protein, Selenide, water dikinase SelD Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN380.121CYT0-0.200913-00NiCytoplasmic861
CIRA_ECOLIP17315
cirA

cir feuA

cir feuAb2155 JW2142 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Colicin I receptor CirAOuter Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Potential)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 10806384 PMID: 20932056 PMID: 19953543 PMID: 10430870Outer Membrane b-barrel proteinHHN/ACell outer membraneOuter Membrane ??-barrel protein (Experimental)SECDSBA,SKPBAMyesyesY260.695SpI2511.8271-00Yn10-20c25/26oOuterMembrane1046
AGP_ECOLIP19926
agp
b1002 JW0987 periplasmic protein (SEC through IM), Glucose-1-phosphatase AgpPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 2153660 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY230.88SpI2221.9324-00Yn5-16c22/23oPeriplasmic1062
TOLA_ECOLIP19934
tolA

cim excC lky

cim excC lkyb0739 JW0729 integral IM protein (SEC in IM), Tol-Pal system TolA Integral Inner Membrane (Experimental)The N-terminal coiled coil of YbgFvforms a stable trimer and controls the oligomeric status of YbgF. The stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein. PMID: 7744737 Disulfide bridge (363 388) Disulfide bond-containing protein PMID: 25664544PMID: 15919657 PMID: 7744737in/outIntegral Inner MembraneBBIM (predicted)Cell inner membrane Membrane anchored (Experimental)SRPSECyesyesN430.131CYT0-0.200913-11Ni12-31oUnknown250
RNI_ECOLIP21338
rna

rnsA

rnsAb0611 JW0603 periplasmic protein (SEC through IM), Ribonuclease I RnaPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbA substrate PMID: 19953543 PMID: 2253883 Disulfide bridge (103 159) PMID: 2253883PeriplasmicGGN/APeriplasm Cytoplasm Periplasmic (Experimental)SECDSBA,DSBCyesyesY240.538SpI2515.6073-00Yn8-18c23/24oPeriplasmic1031
ECOT_ECOLIP23827
eco

eti

etib2209 JW2197 periplasmic protein (SEC through IM), EcoPeriplasmic (Probable) Periplasmic (Experimental) Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY210.889SpI2016.5012-00Yn4-15c20/21oPeriplasmic1085
OPPA_ECOLIP23843
oppA
b1243 JW1235 periplasmic protein (SEC through IM), oligopeptide-binding protein OppAPeriplasmic (Experimental)SecB substrate. Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (297 443) Disulfide bond-containing protein PMID: 25664544PMID: 9298646 PMID: 22030350PeriplasmicGGSoluble IM FractionPeriplasmPeriplasmic (Experimental)SECBSECyesyesY270.825SpI2618.3169-10Yn8-18c26/27oPeriplasmic1073
DPPA_ECOLIP23847
dppA
b3544 JW3513 periplasmic protein (SEC through IM), dipeptide transport DppAPeriplasmic (Probable) Periplasmic (Potential) Periplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (34 262;450 463) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 19953543 PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBA,SKPyesyesY290.638SpI2816.4393-00Yn13-23c28/29oPeriplasmic1085
PSPE_ECOLIP23857
pspE
b1308 JW1301 periplasmic protein (SEC through IM), Thiosulfate sulfurtransferase PspE Periplasmic (Experimental) Periplasmic (Potential)Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 9868784 Disulfide bond-containing protein PMID: 25664544PMID: 9868784 PMID: 1712397PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECProphage TransposaseyesnoY200.9SpI1920.0627-00Yn5-15c19/20oUnknown2
AMY1_ECOLIP25718
malS
b3571 JW3543 periplasmic protein (SEC through IM), alpha-amylase MalSPeriplasmic (Experimental) Disulfide bridge (57 75;121 537) PMID: 9268356 Disulfide bond-containing protein PMID: 25664544PMID: 1544897PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECnoyesY180.764SpI1714.5348-00Yn3-11c17/18oPeriplasmic1025
END1_ECOLIP25736
endA
b2945 JW2912 periplasmic protein (SEC through IM), Endonuclease-1 EndAPeriplasmic (Probable) Periplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 7867949 Disulfide bond-containing protein PMID: 25664544PMID: 4955462 PMID: 7867949PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECDSBAnoyesY230.883SpI2222.5344-00Yn5-16c22/23oUnknown599
MNMA_ECOLIP25745
mnmA

asuE trmU ycfB

asuE trmU ycfBb1133 JW1119 cytoplasmic protein, tRNA-specific 2-thiouridylase MnmA Disulfide bridge (102 199) CytoplasmicAAN/ACytoplasmCytoplasmicyesyesN230.288CYT0-0.200913-00NiCytoplasmic1068
LOIP_ECOLIP25894
loiP

yggG

yggGb2936 JW2903 OM lipoprotein (SEC through IM), inserted in OM by LOL), Metalloprotease LoiP Outer Membrane Lipoprotein (Probable) Outer Membrane Lipoprotein (Experimental) Disulfide bridge (53 108) PMID: 22491786PMID: 17909889 PMID: 22491786 PMID: 20932056Outer Membrane LipoproteinIIN/ACell outer membrane Outer membrane LipoproteinSECLOLyesyesN350.146SpII1825.8287Pos+2=Q00Yn5-16c34/35oUnknown260
MLIC_ECOLIP28224
mliC

ydhA

ydhAb1639 JW1631 OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lysozyme inhibitor of C-type lysozyme MliCOuter Membrane Lipoprotein (Probable) Disulfide bridge (37 102) PMID: 16937244PMID: 18369469Outer Membrane LipoproteinIIN/ACell outer membrane Outer membrane LipoproteinSECLOLyesyesY230.288SpII177.61087Pos+2=S00Yn6-15c20/21oUnknown253
FIMD_ECOLIP30130
fimD
b4317 JW5780 Fimbrial OM b-barrel protein (SEC through IM, chaperone usher export system in OM), FimDOuter Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable)Fim operon (class ??1); Usher. ??uter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Identified in the outer membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 12842468 Disulfide bridge (855 877) PMID: 12488465 PMID: 15911532 PMID: 12842468Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel protein (Experimental)fimbriaeSECCUyesyesY380.415SpI371.20154-10Yn23-38c45/46oOuterMembrane10
THIC_ECOLIP30136
thiC
b3994 JW3958 cytoplasmic protein, Phosphomethylpyrimidine synthase ThiCNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Disulfide bond-containing protein PMID: 25664544CytoplasmicAAIM (predicted-detected)CytoplasmicyesyesN290.108CYT0-0.200913-00NiCytoplasmic910
ARTI_ECOLIP30859
artI
b0863 JW0847 periplasmic protein (SEC through IM), Putative ABC transporter arginine-binding 2 ArtIPeriplasmic (Probable) Periplasmic (Experimental) Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8801422 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 8801422 PMID: 15911532 PMID: 9298646 PMID: 22030350PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY200.883SpI1918.5986-00Yn4-15c19/20oPeriplasmic1076
ARTJ_ECOLIP30860
artJ
b0860 JW0844 periplasmic protein (SEC through IM), ABC transporter arginine-binding 1 ArtJPeriplasmic (Probable) Periplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 8801423 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 8801422 PMID: 8801423 PMID: 22030350PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECDSBAyesyesY200.849SpI1918.9337-00Yn4-15c19/20oPeriplasmic1073
YEDD_ECOLIP31063
yedD
b1928 JW1913 OM lipoprotein (SEC through IM, inserted in OM by LOL), YedDOuter Membrane Lipoprotein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532Outer Membrane LipoproteinIISoluble IM FractionCell membrane Outer membrane Lipoprotein (Experimental)SECDSBALOLnoyesY200.338SpII1512.6143Pos+2=A00Yn4-12c16/17oUnknown2130
DGCZ_ECOLIP31129
dgcZ

ydeG ydeH

ydeG ydeHb1535 JW1528 cytoplasmic protein, Diguanylate cyclase DgcZ Disulfide bond-containing protein PMID: 25664544CytoplasmicAAN/ACytoplasmicyesyesN240.128CYT0-0.200913-00NiCytoplasmic936
POTF_ECOLIP31133
potF
b0854 JW0838 periplasmic protein (SEC through IM), Putrescine-binding periplasmic protein PotFPeriplasmic (Experimental) Disulfide bridge (175 239) PMID: 9651355 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY270.731SpI2615.245-00Yn9-20c26/27oPeriplasmic1061
THIB_ECOLIP31550
thiB

tbpA yabL

tbpA yabLb0068 JW0067 periplasmic protein (SEC through IM), Thiamine-binding periplasmic protein ThiBPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmPeriplasmicSECSURAnoyesY190.685SpI1810.962-00Yn5-13c18/19oPeriplasmic1022
LPTD_ECOLIP31554
lptD

imp ostA yabG

imp ostA yabGb0054 JW0053 OM b-barrel protein (SEC through IM, inserted in OM by BAM), LPS-assembly LptD Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable)The Protein-disulfide Isomerase DsbC Cooperates with SurA and DsbA in the Assembly of LptD. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (31 724;173 725) PMID: 15911532 PMID: 12724388 PMID: 16079137 PMID: 20932056 PMID: 9298646Outer Membrane b-barrel proteinHHSoluble IM FractionCell outer membrane Outer Membrane ??-barrel protein (Experimental)SECDSBA,DSBC,SURA,SKPBAMyesyesY250.626SpI2418.6702-00Yn7-17c24/25oOuterMembrane10
RHAB_ECOLIP32171
rhaB
b3904 JW3875 cytoplasmic protein, L-Rhamnulokinase RhaB Disulfide bridge (68 222;353 370;413 417) CytoplasmicAAN/ACytoplasmicyesyesN210.191CYT0-0.200913-00NiCytoplasmic83
HTRE_ECOLIP33129
htrE
b0139 JW0135 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), HtrEPutative chaperone???usher fimbrial operon (class ??4); Usher. Disulfide bridge (838 862) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMnoyesY300.677SpI296.72527-00NiOuterMembrane108
YEBF_ECOLIP33219
yebF
b1847 JW1836 extra-cellular protein (SEC through IM, secreted in OMV), YebFExtra-cellular (Probable) Extra-cellular (Experimental)Outer membrane proteins OmpF, OmpC, and OmpX are central to the YebF export mechanism. Identified DsbA substrate PMID: 19953543 PMID: 16369539 Disulfide bond-containing protein PMID: 25664544PMID: 16369539Extra-cellularXXN/ASecreted Secreted and released (Experimental)SECOMVnoyesY220.777SpI2120.4773-00Yn5-16c21/22oExtracellular10108
YEHB_ECOLIP33341
yehB
b2109 JW2096 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YehBPutative chaperone???usher fimbrial operon yeh (class ??4); Usher. Disulfide bridge (809 825) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMnoyesY230.679SpI2211.7505-00Yn7-18c22/23oOuterMembrane105
YEHD_ECOLIP33343
yehD
b2111 JW2098 Fimbrial periplasmic protein (SEC through IM), YehDPutative chaperone???usher fimbrial operon yeh (class ??4); Fimbrial Subunit. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicfimbriaeSECnoyesY230.891SpI2224.5442-10Yn4-15c22/23oUnknown295
NAPA_ECOLIP33937
napA

yojC yojD yojE

yojC yojD yojEb2206 JW2194 periplasmic protein (TAT through IM), nitrate reductase NapAPeriplasmic (Probable) Periplasmic (Experimental)TAT secretion system. Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 11844760PeriplasmicGGN/APeriplasm Periplasmic (Experimental)TATyesyesY320.617SpI3120.6365-00Yn13-23c31/32oPeriplasmic10
AHPF_ECOLIP35340
ahpF
b0606 JW0599 cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase subunit F AhpFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (345 348) PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCytoplasmicyesyesN350.108CYT0-0.200913-00NiCytoplasmicMembrane776
DSBD_ECOLIP36655
dsbD

cutA2 cycZ dipZ

cutA2 cycZ dipZb4136 JW5734 integral IM protein (SEC in IM), Thiol:disulfide interchange DsbD Integral Inner Membrane (Experimental)DsbD is an inner membrane protein that is recognised and targeted to the Sec pathway by short, cleavable, N-terminal signal sequence. PMID: 15919996 Disulfide bridge (122 128;182 304;480 483) PMID: 15919996in/outIntegral Inner MembraneBBIM (predicted), Detected in IM FractionCell inner membrane; Multi-pass membrane proteinIntegral Membrane Protein (Experimental)SRPSECyesyesY200.853SpI1910.1575-87Yn5-13c19/20o168-195i207-231o243-267i288-317o329-351i363-381o387-406iCytoplasmicMembrane10
ACNB_ECOLIP36683
acnB

yacI yacJ

yacI yacJb0118 JW0114 cytoplasmic peripheral IM protein, Aconitate hydratase B AcnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 10585860 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10585860Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmic (Experimental)TFyesyesN420.124CYT0-0.200913-00NiCytoplasmic990
RLMN_ECOLIP36979
rlmN

yfgB

yfgBb2517 JW2501 cytoplasmic protein, Dual-specificity RNA methyltransferase RlmNTrigger factor-sensitive aggregation. Disulfide bridge (118 355) CytoplasmicAAN/ACytoplasm CytoplasmicTFyesyesN580.101CYT0-0.200913-00NiCytoplasmic960
BTUF_ECOLIP37028
btuF

yadT

yadTb0158 JW0154 periplasmic protein (SEC through IM), Vitamin B12-binding protein BtuFPeriplasmic (Probable) Disulfide bridge (183 259) Disulfide bond-containing protein PMID: 25664544PMID: 11790740PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECyesyesY230.751SpI2210.8439-00Yn8-18c22/23oPeriplasmic10
YADN_ECOLIP37050
yadN
b0141 JW0137 Fimbrial periplasmic protein (SEC through IM), YadNPutative chaperone???usher fimbrial operon (class ??4); Fimbrial Subunit. Disulfide bridge (41 86) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY240.713SpI2317.9817-00Yn5-15c23/24oExtracellular977
PTKB_ECOLIP37188
gatB
b2093 JW2077 cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIB component GatBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544PMID: 16963640 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasm CytoplasmicnoyesY200.743SpI198.62134-00Yn5-14c19/20oCytoplasmic10
YHJJ_ECOLIP37648
yhjJ
b3527 JW3495 periplasmic protein (SEC through IM), YhjJPeriplasmic (Experimental) Potential Skp substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECSKPyesyesY250.722SpI2414.0569-00Yn8-19c24/25oUnknown283
EPTB_ECOLIP37661
eptB

yhjW

yhjWb3546 JW5660 integral IM protein (SEC in IM), EptBIntegral Inner Membrane (Probable) Integral Inner Membrane (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 15795227 PMID: 15919996in/outIntegral Inner MembraneBBIM (predicted)Cell inner membrane Integral Membrane Protein (Experimental)SRPSECyesyesN380.136TMH011.408-55Ni12-30o42-65i77-103o123-140i161-178oCytoplasmicMembrane10
YIBQ_ECOLIP37691
yibQ
b3614 JW5645 periplasmic protein (SEC through IM), YibQPeriplasmicGGN/APeriplasmicSECyesyesY240.782SpI2313.9145-10Yn8-18c23/24oUnknown214
WBBK_ECOLIP37751
wbbK

yefI

yefIb2032 JW2017 cytoplasmic peripheral IM protein, Putative glycosyltransferase WbbK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Soluble IM FractionCell inner membrane CytoplasmicnoyesN210.119CYT0-0.200913-00NiUnknown232
GLTI_ECOLIP37902
gltI

ybeJ yzzK

ybeJ yzzKb0655 JW5092 periplasmic protein (SEC through IM), Glutamate/aspartate import solute-binding protein GltIPeriplasmic (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 8740179 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 8740179 PMID: 22030350PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECDSBAyesyesY230.775SpI2221.5903-00Yn7-17c22/23oPeriplasmic1093
YBGD_ECOLIP37909
ybgD
b0719 JW0709 Fimbrial periplasmic protein (SEC through IM), YbgDPutative chaperone???usher fimbrial operon yeh (class ??); Fimbrial Subunit. Disulfide bridge (42 81) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY240.878SpI2315.4399-00Yn7-18c23/24oExtracellular9
SFMF_ECOLIP38052
sfmF

ybcG

ybcGb0534 JW5072 Fimbrial periplasmic protein (SEC through IM), SfmFPotential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (40 80) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY210.763SpI2016.3129-00Yn4-15c20/21oExtracellular964
TORT_ECOLIP38683
torT

yccH

yccHb0994 JW0979 periplasmic protein (SEC through IM), Periplasmic TorTPeriplasmic (Experimental)Might be is co-translationally translocated through Sec translocon. PMID: 8576063 The precursor of TorT harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 Disulfide bond-containing protein PMID: 25664544PMID: 8576063 PMID: 24717922 PMID: 15838024PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SRP (exp)SECyesyesY190.789SpI1812.9296-00Yn3-14c18/19oPeriplasmic1012
ZNUA_ECOLIP39172
znuA

yebL yzzP

yebL yzzPb1857 JW5831 periplasmic protein (SEC through IM), High-affinity zinc uptake system ZnuA Periplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY270.573SpI2318.3411-00Yn6-17c26/27oPeriplasmic1084
FIMI_ECOLIP39264
fimI
b4315 JW5779 Fimbrial periplasmic protein (SEC through IM), Fimbrin-like FimIFim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Disulfide bridge (43 83) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY200.716SpI1916.0857-00Yn6-13c19/20oExtracellular937
YTFQ_ECOLIP39325
ytfQ
b4227 JW4186 periplasmic protein (SEC through IM), ABC transporter periplasmic-binding YtfQPeriplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECDSBAyesyesY220.849SpI2121.6548-10Yn5-13c21/22oPeriplasmic1090
OPGB_ECOLIP39401
mdoB

opgB yjjO

opgB yjjOb4359 JW5794 integral IM protein (SEC in IM), Phosphoglycerol transferase I MdoBIntegral Inner Membrane (Probable) Disulfide bond-containing protein PMID: 25664544PMID: 15919996out/outIntegral Inner MembraneBBIM (predicted)Cell inner membrane Integral Membrane ProteinSRPSECnoyesN210.198TMH05.71979-44Yn4-14c18/19o28-47i59-74o80-98i110-128oCytoplasmicMembrane107
RIR3_ECOLIP39452
nrdE
b2675 JW2650 cytoplasmic protein, Ribonucleoside-diphosphate reductase 2 subunit alpha NrdE Disulfide bridge (178 415) CytoplasmicAAN/ACytoplasmicyesyesN300.107CYT0-0.200913-00NiCytoplasmic97
YGIL_ECOLIP39834
ygiL
b3043 JW3011 Fimbrial periplasmic protein (SEC through IM), YgiLPutative chaperone???usher fimbrial operon (class ??); Unknown function. Disulfide bridge (41 80) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY240.867SpI2315.5994-00Yn7-18c23/24oExtracellular992
OPGD_ECOLIP40120
mdoD

opgD ydcG yzzZ

opgD ydcG yzzZb1424 JW1420 periplasmic protein (TAT-SEC through IM), Glucans biosynthesis D MdoDPeriplasmic (Experimental)TAT secretion system. PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)TAT,SECnoyesY330.683SpI3218.7932-00Yn14-25c32/33oPeriplasmic1061
YRAH_ECOLIP42913
yraH
b3142 JW3111 Fimbrial periplasmic protein (SEC through IM), YraHPotential fimbrial yra operon (class ??1); Fimbrial Subunit. Disulfide bridge (38 78) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY230.673SpI2211.7644-00Yn7-17c22/23oExtracellular9
YRAJ_ECOLIP42915
yraJ
b3144 JW3113 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YraJPotential fimbrial yra operon (class ??1); Usher. Disulfide bridge (815 837) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMnoyesN410.51SpI409.33083-00NiOuterMembrane106
YHCD_ECOLIP45420
yhcD
b3216 JW3183 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YhcDPotential fimbrial yhc operon (class ??); Uknown function. Disulfide bridge (769 792) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMnoyesY220.809SpI2110.0887-00Yn5-16c21/22oOuterMembrane10
LPOA_ECOLIP45464
lpoA

yraM

yraMb3147 JW3116 OM lipoprotein (SEC through IM), inserted in OM by LOL), Penicillin-binding activator LpoA Outer Membrane Lipoprotein (Probable) Outer Membrane Lipoprotein (Experimental)The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 21183073 PMID: 21183074 PMID: 20932056Outer Membrane LipoproteinIISoluble IM FractionCell outer membrane Membrane LipoproteinSECLOLnoyesY260.162SpII2612.6937Pos+2=G00Yn14-27c45/46oCytoplasmicMembrane9105
AIS_ECOLIP45565
ais

pmrG

pmrGb2252 JW2246 periplasmic protein (SEC through IM), polysaccharide core heptose AisPeriplasmic ( By similarity)Periplasmic protein by virtue of its homology with the well characterized Salmonella homologue PmrGPMID: 16803591. Disulfide bond-containing protein PMID: 25664544PMID: 16803591PeriplasmicGGN/APeriplasm Membrane anchoredSECyesyesN320.351SpI319.81523-10Yn16-27c31/32oUnknown250
LUXS_ECOLIP45578
luxS

ygaG

ygaGb2687 JW2662 cytoplasmic peripheral IM protein, S-ribosylhomocysteine lyase LuxSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1N/ACytoplasmicTFnoyesN190.106CYT0-0.200913-00NiCytoplasmic8110
YHDW_ECOLIP45766
yhdW
b3268 JW3236 periplasmic protein (SEC through IM), Putative amino-acid ABC transporter-binding YhdW Disulfide bond-containing protein PMID: 25664544PeriplasmicGGSoluble IM FractionPeriplasm CytoplasmicSECpseudogene (Uniprot) nonoY250.628SpI2420.0416-00Yn6-17c24/25oPeriplasmic1089
YBHC_ECOLIP46130
ybhC
b0772 JW0755 OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative acyl-CoA thioester hydrolase YbhC Outer Membrane Lipoprotein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) PMID: 10806384 Disulfide bridge (185 197) PMID: 19452549PMID: 15174130 PMID: 15911532 PMID: 10806384 PMID: 20932056Outer Membrane LipoproteinIISoluble IM FractionCell outer membrane Outer membrane Lipoprotein (Experimental)SECLOLyesyesY240.274SpII2124.9976Pos+2=S00Yn9-19c24/25oOuterMembrane971
CSGC_ECOLIP52107
csgC
b1043 JW1026 periplasmic protein (SEC through IM), Curli assembly CsgCUniprot mentions an 8aa signal peptide but Signal P prediction is for a "typical" 22 residue peptide Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasm PeriplasmicSECnoyesN220.179SpI222.07115-00Yn4-16c21/22oUnknown268
RZOD_ECOLIP58041
rzoD
b4510 JW5080 OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage RzoD (o-spanin) Disulfide bridge (29 29) Outer Membrane LipoproteinIIN/ACell outer membrane Outer membrane LipoproteinSECLOLProphage Integrase (EcoGene)nonoY200.187SpII1919.9508Pos+2=T00Yn8-18c22/23oUnknown2147
RZOR_ECOLIP58042
rzoR
b4528 JW5213 OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage RzoR (o-spanin) Disulfide bridge (29 29) Outer Membrane LipoproteinIIN/ACell outer membrane Outer membrane LipoproteinSECLOLProphage Integrase (EcoGene)nonoY200.189SpII1920.8844Pos+2=T00Yn8-18c22/23oUnknown288
DPAL_ECOLIP66899
ygeX
b2871 JW2839 cytoplasmic protein, Diaminopropionate ammonia-lyase YgeX Disulfide bridge (265 291) PMID: 22505717CytoplasmicAAN/ACytoplasmicyesyesN330.114CYT0-0.200913-00NiCytoplasmic975
RCSF_ECOLIP69411
rcsF
b0196 JW0192 OM lipoprotein (SEC through IM, inserted in OM by LOL), RcsFOuter Membrane Lipoprotein (Experimental) Outer Membrane Lipoprotein (Probable) Proven pignal peptidase II substrate. Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bridge (74 118;109 124) Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 16740933 PMID: 23813676 PMID: 20932056Outer Membrane LipoproteinIISoluble IM FractionCell outer membrane Outer membrane Lipoprotein (Experimental)SECDSBALOLyesyesY230.182SpII1513.2891Pos+2=S00Yn3-13c21/22oOuterMembrane1081
YAHK_ECOLIP75691
yahK
b0325 JW0317 cytoplasmic peripheral IM protein, Aldehyde reductase YahKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1IM (predicted-detected)CytoplasmicnoyesN530.148CYT0-0.200913-00NiCytoplasmic973
YBGO_ECOLIP75748
ybgO
b0716 JW5098 Fimbrial periplasmic protein (SEC through IM), YbgOPutative chaperone???usher fimbrial operon yeh (class ??); Unkown function. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicfimbriaeSECnoyesN240.359SpI237.76951-00Yn7-18c23/24oCytoplasmic853
YBGQ_ECOLIP75750
ybgQ
b0718 JW5099 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YbgQPutative chaperone???usher fimbrial operon yeh (class ??); Usher. Disulfide bridge (795 814) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMnoyesY220.84SpI218.29107-00Yn6-16c21/22oOuterMembrane1010
FIU_ECOLIP75780
fiu

ybiL

ybiLb0805 JW0790 OM b-barrel protein (SEC through IM, inserted in OM by BAM), Catecholate siderophore receptor FiuOuter Membrane b-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 10806384 PMID: 20932056Outer Membrane b-barrel proteinHHN/ACell outer membraneOuter Membrane ??-barrel protein (Experimental)SECSKPBAMyesyesY340.525SpI3311.8291-10Yn14-25c33/34oOuterMembrane1010
YBJP_ECOLIP75818
ybjP
b0865 JW0849 OM lipoprotein (SEC through IM, inserted in OM by LOL), YbjPOuter Membrane Lipoprotein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Identified DsbA substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 10806384Outer Membrane LipoproteinIISoluble IM FractionCell membrane Outer membrane Lipoprotein (Experimental)SECDSBALOLnoyesY280.445SpII1810.6734Pos+2=T00Yn9-19c24/25oUnknown269
YDCS_ECOLIP76108
ydcS
b1440 JW1435 periplasmic protein (SEC through IM), Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding YdcSPeriplasmic (Experimental) Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 22030350PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECnoyesY230.836SpI2217.8592-00Yn10-18c22/23oPeriplasmic1016
YNFB_ECOLIP76170
ynfB
b1583 JW1575 periplasmic peripheral OM protein (SEC through IM), UPF0482 YnfBPeripheral outer membrane protein facing the periplasm (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544PMID: 15911532Peripheral outer membrane protein facing the periplasmF3F3N/APeriplasmicSECyesyesY290.87SpI2819.5483-00Yn9-20c28/29oUnknown249
ZINT_ECOLIP76344
zinT

yodA

yodAb1973 JW1956 periplasmic protein (SEC through IM), Metal-binding ZinT Periplasmic (Probable) Periplasmic (Experimental)After 45 min of cadmium exposure the YodA protein was primarily detected in the cytoplasmic fraction but was later (150 min) found in both the cytoplasmic and periplasmic compartments. PMID: 9579078 Disulfide bond-containing protein PMID: 25664544PMID: 12480884 PMID: 9579078PeriplasmicGGN/ACytoplasm Periplasmic (Experimental)SECyesyesY240.683SpI2312.2997-00Yn8-18c23/24oUnknown2
YFCP_ECOLIP76499
yfcP
b2333 JW2330 Fimbrial periplasmic protein (SEC through IM), YfcPPutative chaperone???usher fimbrial operon yfc (class ??); Fimbrial Subunit. Disulfide bridge (43 83) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUyesyesY280.79SpI2714.214-10Yn8-19c27/28oUnknown2
YFEY_ECOLIP76537
yfeY
b2432 JW2425 OM lipoprotein (SEC through IM, inserted in OM by LOL), YfeYOuter Membrane Lipoprotein (Experimental) Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130 Disulfide bond-containing protein PMID: 25664544PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIIN/AOuter membrane Lipoprotein (Experimental)SECLOLyesyesY290.248SpII1720.3796Pos+2=S00Yn8-19c27/28oUnknown259
YPFG_ECOLIP76559
ypfG
b2466 JW2450 periplasmic protein (SEC through IM), YpfG Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicSECyesyesY220.804SpI2115.2807-10Yn5-16c21/22oCytoplasmicMembrane961
YFHM_ECOLIP76578
yfhM
b2520 JW2504 IM lipoprotein (SEC through IM), inserted in OM by LOL), Alpha-2-macroglobulin YfhM Identified DsbA substrate PMID: 19953543Inner Membrane LipoproteinEESoluble IM FractionCell inner membrane Inner membrane lipoproteinSECDSBALOLyesyesY250.18SpII1728.7232Pos+2=D00Yn6-13c18/19oUnknown4
YQIG_ECOLIP76655
yqiG
b3046 JW5507 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative usher YqiG Putative chaperone???usher fimbrial operon (class ??); Usher. Disulfide bridge (798 817) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMpseudogene (Uniprot)noyesN210.333CYT0-0.200913-00NiOuterMembrane10
DSBG_ECOLIP77202
dsbG

ybdP

ybdPb0604 JW0597 periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbG Periplasmic (Experimental) Disulfide bridge (126 129) PMID: 15184683 Disulfide bond-containing protein PMID: 25664544PMID: 9383195PeriplasmicGGN/APeriplasmPeriplasmic (Experimental)SECyesyesY180.618SpI1712.1105-00Yn5-12c17/18oPeriplasmic1050
RCLA_ECOLIP77212
rclA

ykgC

ykgCb0304 JW5040 cytoplasmic protein, Probable pyridine nucleotide-disulfide oxidoreductase RclA Disulfide bridge (43 48) CytoplasmicAAN/ACytoplasmicnoyesN200.197CYT0-0.200913-00NiCytoplasmic946
ECPE_ECOLIP77263
ecpE

matF yagV

matF yagVb0289 JW5030 Fimbrial periplasmic protein (SEC through IM), Probable chaperone EcpEPutative chaperone???usher fimbrial operon yag(class ??); Unknown function. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/APeriplasmicfimbriaeSECSURAnoyesY280.534SpI278.52618-10Yn12-22c27/28oUnknown217
YPHF_ECOLIP77269
yphF
b2548 JW2532 periplasmic protein (SEC through IM), ABC transporter periplasmic-binding YphFPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECnoyesY270.817SpI2620.8859-00Yn11-22c26/27oPeriplasmic963
RSXG_ECOLIP77285
rsxG

rnfG ydgP

rnfG ydgPb1631 JW1623 periplasmic protein (SEC through IM), Ion-translocating oxidoreductase complex subunit G RsxG Disulfide bond-containing protein PMID: 25664544PeriplasmicGGN/ACell inner membrane PeriplasmicSECyesyesY250.427SpI2411.9411-00Yn9-17c24/25oUnknown242
YDER_ECOLIP77294
ydeR
b1503 JW1497 Fimbrial periplasmic protein (SEC through IM), YdeRPotential fimbrial yde operon (class ??1); Fimbrial Subunit. Disulfide bridge (39 77) PeriplasmicGGN/AFimbrium PeriplasmicfimbriaeSECCUnoyesY240.701SpI2320.0135-00Yn8-18c23/24oUnknown267
BORD_ECOLIP77330
borD

ybcU

ybcUb0557 JW0546 OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage Bor homolog BorDOuter Membrane Lipoprotein (Experimental)Proven pignal peptidase II substrate. PMID: 15174130 Disulfide bond-containing protein PMID: 25664544PMID: 15174130Outer Membrane LipoproteinIISoluble IM FractionCell membrane Outer membrane Lipoprotein (Experimental)SECLOLProphage Integrase (EcoGene)nonoY210.303SpII1621.3732Pos+2=A00Yn5-14c18/19oUnknown232
HLYE_ECOLIP77335
hlyE

clyA hpr sheA ycgD

clyA hpr sheA ycgDb1182 JW5181 periplasmic & extra-cellular protein (SEC through IM), secreted in OMV), Hemolysin E, chromosomal HlyEPeriplasmic, Extra-cellular (Potential) Periplasmic, Extra-cellular (Probable) Periplasmic, Extra-cellular (Experimental)HlyE(ClyA) forms a dodecameric transmembrane pore. The ability of ClyA to translocate to the periplasm is abolished in deletion mutants lacking the last 23 or 11 amino acid residues of the C-terminal region. Also, analysis of ClyA mutants with defined deletions and amino acid exchanges revealed that several ClyA segments which are distant of each other in the primary structure, namely, the N-terminal ??A1 region, the C-terminal region, and the hydrophobic sequence ranging from residues 89 to 101, are crucial for ClyA translocation from the E. coli cytoplasm to the periplasm. In the soluble ClyA monomer, these regions are found closely adjacent to each other in the tail domain. ClyA assembles into an oligomeric structure in solution in the absence of either bilayer membranes or detergents at physiological temperature.These oligomers can rearrange to create transmembrane pores when in contact with detergents or biological membranes. ClyA does not form transmembrane pores on E. coli lipid membranes. Recombinant E. coli overexpressing the cloned clyA gene accumulated this haemolysin in the periplasm and released only very small amounts of it into the external medium. PMID: 11902713 Disulfide bridge (87 285) PMID: 14532000PMID: 20511497 PMID: 10027972 PMID: 11902713Periplasmic, Extra-cellularG, XG,XN/ASecreted Periplasm Host cell membrane Secreted and released (Experimental)SECOMVnoyesN580.117CYT0-0.200913-10NiExtracellular1062
YBCL_ECOLIP77368
ybcL
b0545 JW0533 periplasmic protein (SEC through IM), UPF0098 YbcLPeriplasmic (Probable) Disulfide bridge (46 129) Disulfide bond-containing protein PMID: 25664544PMID: 11439028PeriplasmicGGN/APeriplasm Periplasmic (Experimental)SECProphage Integrase (EcoGene)yesnoY200.717SpI1919.8157-00Yn4-15c19/20oPeriplasmic1048
RCLR_ECOLIP77379
rclR

ykgD

ykgDb0305 JW0298 nucleoid protein, RCS-specific HTH-type transcriptional activator RclR Cytoplasmic, Nucleoid (Experimental) Disulfide bridge (21 89) PMID: 24078635 Disulfide bridge (21 89) PMID: 24078635PMID: 23138451Cytoplasmic, NucleoidA, NA,NIM (predicted)CytoplasmicnoyesN340.112CYT0-0.200913-00NiUnknown213
SFMD_ECOLIP77468
sfmD
b0532 JW0521 Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), SfmDPotential fimbrial sfm operon (class ??1); Usher. Disulfide bridge (840 862) Outer Membrane b-barrel proteinHHN/ACell outer membrane Outer Membrane ??-barrel proteinfimbriaeSECBAMyesyesN360.404TMH03.9822-11Ni12-32oOuterMembrane10
YAIW_ECOLIP77562
yaiW
b0378 JW0369 OM lipoprotein (SEC through IM, inserted in OM by LOL), YaiWOuter Membrane Lipoprotein (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 20932056Outer Membrane LipoproteinIIN/AOuter membrane LipoproteinSECLOLnoyesY260.354SpII2028.9818Pos+2=S00Yn7-18c23/24oUnknown210
YKGA_ECOLIP77601
ykgA
b0300 JW5037 nucleoid protein, Putative HTH-type transcriptional regulator YkgA Cytoplasmic, Nucleoid (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23138451Cytoplasmic, NucleoidA, NA,NN/ACytoplasmicpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN560.1CYT0-0.200913-00NiCytoplasmic914
THII_ECOLIP77718
thiI

yajK

yajKb0423 JW0413 cytoplasmic protein, tRNA sulfurtransferase ThiI Disulfide bridge (344 456) PMID: 16855700CytoplasmicAAN/ACytoplasmCytoplasmicnoyesN550.111CYT0-0.200913-00NiCytoplasmic10
YDES_ECOLIP77789
ydeS
b1504 JW1498 Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), YdeSPotential fimbrial yde operon (class ??1); Fimbrial Subunit. Disulfide bridge (38 78) Peripheral outer membrane protein facing the extra-cellular spaceF4F4N/AFimbrium Membrane anchoredfimbriaeSECCUnoyesY230.426SpI2211.5353-10Yn6-17c22/23oExtracellular1079
COPA_ECOLIQ59385
copA

atcU f834 ybaR

atcU f834 ybaRb0484 JW0473 integral IM protein (SEC-YidC in IM), Copper-exporting P-type ATPase CopAIntegral Inner Membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 10639134 Disulfide bond-containing protein PMID: 25664544PMID: 15919996 PMID: 10639134in/inIntegral Inner MembraneBBIM (predicted), Detected in IM FractionCopper-exporting P-type ATPase: Cell inner membrane Integral Membrane Protein (Experimental)SRPSEC-YidCnoyesN500.162CYT0-0.200913-88Ni185-205o217-238i245-264o284-302i436-458o464-487i778-797o803-824iCytoplasmicMembrane1021