entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermsubloci_uniprotsubloci_echoLbernselorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
FABZ_ECOLIP0A6Q6
fabZ

sefA yaeA

sefA yaeAb0180 JW0175 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase ((3R)-hydroxymyristoyl ACP dehydrase) (EC 4.2.1.-) (17 kDa actomyosin component)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 8354462 PMID: 7806516 PMID: 8805534 PMID: 8910376 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15911532 PMID: 8910376 PMID: 8354462 PMID: 7806516 PMID: 8805534 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmic (Experimental)N/ATFyesyesNo320.104CYT0-0.200913-00="0"iCytoplasmic1027.87
MIAB_ECOLIP0AEI1
miaB

yleA

yleAb0661 JW0658 (Dimethylallyl)adenosine tRNA methylthiotransferase miaB (EC 2.-.-.-) (tRNA-i(6)A37 methylthiotransferase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo560.146CYT0-0.200913-00="0"iCytoplasmic9.9770.61
GLGB_ECOLIP07762
glgB
b3432 JW3395 1,4-alpha-glucan-branching enzyme (EC 2.4.1.18) (1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase) (Glycogen-branching enzyme) (BE)CytoplasmicAACytoplasmicN/AyesyesNo270.139CYT0-0.200913-00="0"iCytoplasmic9.9718.15
MENA_ECOLIP32166
menA

yiiW

yiiWb3930 JW3901 1,4-dihydroxy-2-naphthoate octaprenyltransferase (DHNA-octaprenyltransferase) (EC 2.5.1.74)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECyesyesNo360.309TMH04.61773-99="0"i21-38o44-63i98-118o124-143i150-172o178-199i228-246o252-270i290-307oCytoplasmicMembrane1014.32
AMPD_ECOLIP13016
ampD
b0110 JW0106 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC 3.5.1.28) (N-acetylmuramoyl-L-alanine amidase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo450.136CYT0-0.200913-00="0"iCytoplasmic1060.06
HIS4_ECOLIP10371
hisA
b2024 JW2006 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16) (Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo550.11CYT0-0.200913-00="0"iCytoplasmic1044.27
PLSC_ECOLIP26647
plsC

parF

parFb3018 JW2986 1-acyl-sn-glycerol-3-phosphate acyltransferase (1-AGP acyltransferase) (1-AGPAT) (EC 2.3.1.51) (EC 2.3.1.n4) (Lysophosphatidic acid acyltransferase) (LPAAT) (Phosphatidic acid synthase) (PA synthase)Integral Inner Membrane (Probable)PMID: 1557036Integral Inner Membrane BBin/outCell inner membrane | Peripheral membrane proteinMembrane anchored (Experimental)IM (predicted)SECyesyesNo360.111TMH01.55803-11="0"o6-27iCytoplasmicMembrane1028.89
DXR_ECOLIP45568
dxr

ispC yaeM

ispC yaeMb0173 JW0168 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP reductoisomerase) (EC 1.1.1.267) (1-deoxyxylulose-5-phosphate reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate synthase)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo550.115CYT0-0.200913-00="0"iCytoplasmicMembrane9.82
DXS_ECOLIP77488
dxs

yajP

yajPb0420 JW0410 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7) (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental) YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300PMID: 23230279 PMID: 17272300Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo520.114CYT0-0.200913-00="0"iCytoplasmic9.9721.77
K1PF_ECOLIP0AEW9
fruK

fpk

fpkb2168 JW2155 1-phosphofructokinase (EC 2.7.1.56) (Fructose 1-phosphate kinase)CytoplasmicAACytoplasmicN/AnoyesNo390.121CYT0-0.200913-00="0"iCytoplasmic9.2679.32
CH10_ECOLIP0A6F9
groS

groES mopB

groES mopBb4142 JW4102 10 kDa chaperonin (Protein Cpn10) (groES protein)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental) (Experimental) Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 23230279 PMID: 22380631 PMID: 20153451Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AnoyesNo430.185CYT0-0.200913-00="0"iCytoplasmic1086.32
CPDB_ECOLIP08331
cpdB
b4213 JW4171 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC 3.1.3.6) (EC 3.1.4.16)Periplasm (Probable) Periplasm (Experimental) Periplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 3005231 PMID: 15911532 PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECSurAnoyesYes200.787SpI1914.5002-00Yn4-14c19/20oPeriplasmic1082.97
LIGT_ECOLIP37025
ligT

yadP

yadPb0147 JW5011 2'-5'-RNA ligase (EC 6.5.1.-)CytoplasmicAACytoplasmicN/AnoyesNo400.11CYT0-0.200913-00="0"iUnknown279.3
DAPD_ECOLIP0A9D8
dapD
b0166 JW0161 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117) (Tetrahydrodipicolinate N-succinyltransferase) (THDP succinyltransferase) (THP succinyltransferase) (Tetrahydropicolinate succinylase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo240.195CYT0-0.200913-00="0"iCytoplasmic1041.15
GPMA_ECOLIP62707
gpmA

gpm pgm pgmA

gpm pgm pgmAb0755 JW0738 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (BPG-dependent PGAM) (PGAM) (Phosphoglyceromutase) (dPGM) (EC 5.4.2.1)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo410.153CYT0-0.200913-00="0"iCytoplasmic8.96102.79
GPMI_ECOLIP37689
gpmI

pgmI yibO

pgmI yibOb3612 JW3587 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (BPG-independent PGAM) (Phosphoglyceromutase) (iPGM) (EC 5.4.2.1)CytoplasmicAACytoplasmicN/AyesyesNo200.111CYT0-0.200913-00="0"iCytoplasmic10104.04
ENTA_ECOLIP15047
entA
b0596 JW0588 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28)CytoplasmicAACytoplasmicN/AyesyesNo310.19CYT0-0.200913-00="0"iCytoplasmic9.2663.13
MHPB_ECOLIP0ABR9
mhpB
b0348 JW0339 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (EC 1.13.11.16)CytoplasmicAACytoplasmicN/AnoyesNo230.132CYT0-0.200913-00="0"iCytoplasmicMembrane9.828.2
DLGD_ECOLIP37672
dlgD

yiaK

yiaKb3575 JW3547 2,3-diketo-L-gulonate reductase (2,3-DKG reductase) (EC 1.1.1.130) (3-dehydro-L-gulonate 2-dehydrogenase)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo290.127CYT0-0.200913-00="0"iCytoplasmic9.9736.99
YIAN_ECOLIP37675
yiaN
b3578 JW5651 2,3-diketo-L-gulonate TRAP transporter large permease protein yiaNIntegral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo660.188TMH016.4337-1111="0"o12-35i56-75o95-124i136-158o170-191i212-234o240-256i277-295o315-343i355-375o395-419iCytoplasmicMembrane10
YIAM_ECOLIP37674
yiaM
b3577 JW3549 2,3-diketo-L-gulonate TRAP transporter small permease protein yiaMIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo230.132TMH06.97669-44="0"i7-28o40-59i80-101o113-137iCytoplasmicMembrane1013.66
YIAO_ECOLIP37676
yiaO
b3579 JW3551 2,3-diketo-L-gulonate-binding periplasmic protein yiaO (2,3-DKG-binding protein) (Extracytoplasmic solute receptor protein yiaO)Periplasm (Probable)PMID: 16385129PeriplasmicGGSecretedPeriplasmicN/ASECnoyesYes250.815SpI2415.7433-00Yn6-17c24/25oPeriplasmic1020.37
FADH_ECOLIP42593
fadH

ygjL

ygjLb3081 JW3052 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) (2,4-dienoyl coenzyme A reductase)CytoplasmicAACytoplasmicN/AyesyesNo200.11CYT0-0.200913-00="0"iCytoplasmic9.9717.09
DKGA_ECOLIQ46857
dkgA

yqhE

yqhEb3012 JW5499 2,5-diketo-D-gluconic acid reductase A (2,5-DKG reductase A) (2,5-DKGR A) (25DKGR-A) (EC 1.1.1.274) (AKR5C)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo260.118CYT0-0.200913-00="0"iCytoplasmic9.9727.53
DKGB_ECOLIP30863
dkgB

yafB

yafBb0207 JW0197 2,5-diketo-D-gluconic acid reductase B (2,5-DKG reductase B) (2,5-DKGR B) (25DKGR-B) (EC 1.1.1.274) (AKR5D)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo370.144CYT0-0.200913-00="0"iCytoplasmic9.9778.61
CITG_ECOLIP77231
citG

ybdT

ybdTb0613 JW0605 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (2-(5''-triphosphoribosyl)-3'-dephospho-CoA synthase) (EC 2.7.8.25)CytoplasmicAACytoplasmicIM (predicted)yesyesNo650.116CYT0-0.200913-00="0"iUnknown25.59
KBL_ECOLIP0AB77
kbl
b3617 JW3592 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (EC 2.3.1.29) (Glycine acetyltransferase)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo340.12CYT0-0.200913-00="0"iCytoplasmic9.9737.95
HPPK_ECOLIP26281
folK
b0142 JW0138 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase) (PPPK) (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK)CytoplasmicAACytoplasmicN/AyesyesNo220.266CYT0-0.200913-00="0"iCytoplasmic9.9766.52
ISPF_ECOLIP62617
ispF

mecS ygbB

mecS ygbBb2746 JW2716 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECDP-synthase) (MECPS) (EC 4.6.1.12)CytoplasmicAACytoplasmicN/AyesyesNo120.113CYT0-0.200913-00="0"iCytoplasmic9.2684.05
ISPD_ECOLIQ46893
ispD

ygbP

ygbPb2747 JW2717 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (CDP-ME synthase) (MEP cytidylyltransferase) (MCT)Copurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmicN/ATFyesyesNo470.108CYT0-0.200913-00="0"iCytoplasmic9.9767.12
DGOA_ECOLIQ6BF16
dgoA

yidU

yidUb4477 JW5628 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (EC 4.1.2.21) (2-oxo-3-deoxygalactonate 6-phosphate aldolase) (6-phospho-2-dehydro-3-deoxygalactonate aldolase)CytoplasmicAACytoplasmicN/AnoyesNo240.37CYT0-0.200913-00="0"iCytoplasmic9.9731.8
KDUD_ECOLIP37769
kduD

ygeC yqeD

ygeC yqeDb2842 JW2810 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (EC 1.1.1.127) (2-keto-3-deoxygluconate 5-dehydrogenase) (2-keto-3-deoxygluconate oxidoreductase) (KDG oxidoreductase)CytoplasmicAACytoplasmicN/AyesyesNo340.193CYT0-0.200913-00="0"iCytoplasmic1070.92
DGOK_ECOLIP31459
dgoK

yidV

yidVb3693 JW3670 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58) (2-keto-3-deoxy-galactonokinase) (2-oxo-3-deoxygalactonate kinase)CytoplasmicAACytoplasmicIM (predicted)noyesNo340.174CYT0-0.200913-00="0"iUnknown27.26
KDGK_ECOLIP37647
kdgK

yhjI

yhjIb3526 JW5668 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) (2-keto-3-deoxygluconokinase) (3-deoxy-2-oxo-D-gluconate kinase) (KDG kinase)CytoplasmicAACytoplasmicN/AyesyesNo540.131CYT0-0.200913-00="0"iUnknown2
KDSA_ECOLIP0A715
kdsA
b1215 JW1206 2-dehydro-3-deoxyphosphooctonate aldolase (EC 2.5.1.55) (3-deoxy-D-manno-octulosonic acid 8-phosphate synthase) (KDO-8-phosphate synthase) (KDO 8-P synthase) (KDOPS) (Phospho-2-dehydro-3-deoxyoctonate aldolase)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo560.121CYT0-0.200913-00="0"iCytoplasmic1029.78
PANE_ECOLIP0A9J4
panE

apbA

apbAb0425 JW0415 2-dehydropantoate 2-reductase (EC 1.1.1.169) (Ketopantoate reductase) (KPA reductase) (KPR)Lipoprotein (By similarity) Cytoplasmic (Experimental)Phobious predicts the sequence that follows signal peptide as non cytoplasmic and non transmembrane. The native enzyme exists in solution as a monomer with a molecular mass of 34 000 Da. PMID: 10736170 Lipoprotein PMID: 10572132 PMID: 15174130 PMID: 10736170PMID: 10572132 PMID: 15174130Outer Membrane LipoproteinIICytoplasmCytoplasmic (Experimental)IM (predicted)SECLOL: Localization Of LipoproteinsyesyesNo210.133SpII192.5798Pos+2=K00Yn5-16c20/21oCytoplasmic8.9631.23
GARR_ECOLIP0ABQ2
garR

yhaE

yhaEb3125 JW5526 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60) (Tartronate semialdehyde reductase) (TSAR)CytoplasmicAACytoplasmicN/AyesyesNo380.214CYT0-0.200913-00="0"iCytoplasmic9.9790.88
GLXR_ECOLIP77161
glxR

glxB1 ybbQ

glxB1 ybbQb0509 JW0497 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60) (Tartronate semialdehyde reductase) (TSAR)CytoplasmicAACytoplasmicN/AyesyesNo230.151CYT0-0.200913-00Yn3-10c22/23oCytoplasmic9.9771.6
MHPC_ECOLIP77044
mhpC
b0349 JW0340 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (EC 3.7.1.n1) (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) (2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase) (2-hydroxy-6-oxonona-2,4-diene-1,9-dioic aCytoplasmicAACytoplasmicN/AnoyesNo520.107CYT0-0.200913-00="0"iCytoplasmic8.9616.54
LEU1_ECOLIP09151
leuA
b0074 JW0073 2-isopropylmalate synthase (EC 2.3.3.13) (Alpha-IPM synthase) (Alpha-isopropylmalate synthase)CytoplasmicAACytoplasmicN/AnoyesNo350.106CYT0-0.200913-00="0"iCytoplasmic9.979.56
RHMA_ECOLIP76469
rhmA

yfaU

yfaUb2245 JW2239 2-keto-3-deoxy-L-rhamnonate aldolase (KDR aldolase) (EC 4.1.2.n3) (2-dehydro-3-deoxyrhamnonate aldolase) (2-keto-3-deoxy acid sugar aldolase)CytoplasmicAACytoplasmicIM (predicted)yesyesNo320.417CYT0-0.200913-00="0"iCytoplasmic9.266.94
KDGT_ECOLIP0A712
kdgT
b3909 JW5560 2-keto-3-deoxygluconate permease (KDG permease)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo670.134TMH012.8678-1010="0"i12-29o35-57i78-97o103-127i139-164o170-187i194-211o223-243i255-274o286-309iCytoplasmicMembrane10
MHPD_ECOLIP77608
mhpD
b0350 JW0341 2-keto-4-pentenoate hydratase (EC 4.2.1.80) (2-hydroxypentadienoic acid hydratase)CytoplasmicAACytoplasmicN/AnoyesNo380.173CYT0-0.200913-00="0"iCytoplasmic8.9674.85
PRPD_ECOLIP77243
prpD

yahT

yahTb0334 JW0325 2-methylcitrate dehydratase (EC 4.2.1.79)CytoplasmicAACytoplasmicN/AnoyesNo340.119CYT0-0.200913-00="0"iCytoplasmic9.9710.29
PRPC_ECOLIP31660
prpC

yahS yzzD

yahS yzzDb0333 JW0324 2-methylcitrate synthase (EC 2.3.3.5) (Citrate synthase 2) (Methylcitrate synthase)CytoplasmicAACytoplasmicN/AnoyesNo340.114CYT0-0.200913-00="0"iCytoplasmic9.9792.5
UBIF_ECOLIP75728
ubiF

yleB

yleBb0662 JW0659 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)yesyesNo310.178TMH01.41397-00="0"iUnknown214.14
UBIH_ECOLIP25534
ubiH

visB

visBb2907 JW2875 2-octaprenyl-6-methoxyphenol hydroxylase (EC 1.14.13.-)Peripheral inner membrane protein facing the cytoplasm (Probable)The biosynthesis of UQ is studied in great detail in bacteria and yeast but only to a limited extent in animal tissues. The UQ biosynthetic enzymes may constitute a complex that is tightly bound to the membrane.PMID: 11583838Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo420.38SpI251.25365-00Yn3-13c18/19oUnknown6.4915.42
ODO1_ECOLIP0AFG3
sucA
b0726 JW0715 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Inner membrane (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279 PMID: 15919657Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo240.133CYT0-0.200913-00="0"iCytoplasmic9.976.61
MEND_ECOLIP17109
menD
b2264 JW5374 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (SEPHCHC synthase) (EC 2.2.1.9) (Menaquinone biosynthesis protein menD)CytoplasmicAACytoplasmicN/AyesyesNo410.136CYT0-0.200913-00="0"iCytoplasmicMembrane9.823.8
MENH_ECOLIP37355
menH

yfbB

yfbBb2263 JW2258 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) (EC 4.2.99.20)CytoplasmicAACytoplasmicN/AyesyesNo300.146CYT0-0.200913-00="0"iUnknown2
MENE_ECOLIP37353
menE
b2260 JW2255 2-succinylbenzoate--CoA ligase (EC 6.2.1.26) (o-succinylbenzoyl-CoA synthetase) (OSB-CoA synthetase)Peripheral inner membrane protein facing the cytoplasm (By similarity)Homologous to FadD13 protein of Mycobacterium that is peripheral.PMID: 22560731Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo480.113CYT0-0.200913-00="0"iCytoplasmic9.9731.4
RLMB_ECOLIP63177
rlmB

yjfH

yjfHb4180 JW4138 23S rRNA (guanosine-2'-O-)-methyltransferase rlmB (EC 2.1.1.-) (23S rRNA Gm2251 2'-O-methyltransferase)CytoplasmicAACytoplasmicN/AyesyesNo240.115CYT0-0.200913-00="0"iCytoplasmic9.9793.4
RLMD_ECOLIP55135
rlmD

rumA ygcA

rumA ygcAb2785 JW2756 23S rRNA (uracil-5-)-methyltransferase rumA (EC 2.1.1.-) (23S rRNA(M-5-U1939)-methyltransferase)CytoplasmicAACytoplasmicN/AnoyesNo510.112CYT0-0.200913-00="0"iCytoplasmic9.97
RLMC_ECOLIP75817
rlmC

rumB ybjF

rumB ybjFb0859 JW0843 23S rRNA (uracil-5-)-methyltransferase rumB (EC 2.1.1.-) (23S rRNA(M-5-U747)-methyltransferase)Translation, Ribosomal.CytoplasmicAACytoplasmicN/AyesyesNo280.107CYT0-0.200913-00="0"iCytoplasmic9.9715.5
FER_ECOLIP0A9R4
fdx
b2525 JW2509 2Fe-2S ferredoxinCytoplasmicAACytoplasmicN/AyesyesNo420.124CYT0-0.200913-00="0"iCytoplasmic8.9682.3
CYSQ_ECOLIP22255
cysQ

amtA

amtAb4214 JW4172 3'(2'),5'-bisphosphate nucleotidase cysQ (EC 3.1.3.7) (3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase) (3'-phosphoadenosine 5'-phosphate phosphatase) (PAP phosphatase) (DPNPase)Peripheral inner membrane protein facing the cytoplasm (Probable) Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 15911532PMID: 15911532Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideMembrane associated (Experimental)N/AnoyesNo400.152CYT0-0.200913-00="0"iCytoplasmic9.2683.82
RIBB_ECOLIP0A7J0
ribB

htrP

htrPb3041 JW3009 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) (EC 4.1.99.12)Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo220.191CYT0-0.200913-00="0"iCytoplasmic9.97116.29
MHPA_ECOLIP77397
mhpA
b0347 JW0338 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (3-HCI hydroxylase) (3-HPP hydroxylase) (EC 1.14.13.n3)CytoplasmicAACytoplasmicIM (predicted)noyesNo320.135CYT0-0.200913-00="0"iUnknown27.47
AROD_ECOLIP05194
aroD
b1693 JW1683 3-dehydroquinate dehydratase (3-dehydroquinase) (EC 4.2.1.10) (Type I DHQase)CytoplasmicAACytoplasmicN/AyesyesNo370.183CYT0-0.200913-00="0"iCytoplasmic8.9697.1
AROB_ECOLIP07639
aroB
b3389 JW3352 3-dehydroquinate synthase (EC 4.2.3.4)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo290.12CYT0-0.200913-00="0"iCytoplasmic1022.48
UBIG_ECOLIP17993
ubiG

pufX yfaB

pufX yfaBb2232 JW2226 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64) (3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase) (DHHB methyltransferase)CytoplasmicAACytoplasmicN/AyesyesNo250.101CYT0-0.200913-00="0"iCytoplasmic9.9778.87
KDSC_ECOLIP0ABZ4
kdsC

yrbI yrbJ

yrbI yrbJb3198 JW3165 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45) (KDO 8-P phosphatase)Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Potential)Short signal peptide of 17aa. Prediction by Phobius and the SignalP NN approach/extensive islands of hydrophobicity by K/D. No obvious biochemical reason that the enzyme should be secreted. Signal peptide like sequence could act as peripheral membrane association element. Lipopolysaccharide (LPS) is an amphiphilic molecule composed of a hydrophilic heteropolysaccharide and the membrane-embedded Lipid A. The innermost 8-carbon sugar 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) is the only conserved structural element found in all LPS, linking the lipid A to the carbohydrate domain of the molecule. The KDO 8-phosphate phosphatase (KdsC) is one of the enzymes of the KDO biosynthesis pathway and is responsible for cleavage of the phosphate group from KDO 8-phosphate to yield KDO. PMID: 12639950 In Escherichia coli, KdsC has been characterized as a homotetrameric molecule with a random-coiled C-terminal tail in each subunit. PMID: 12639950 PMID: 12639950PMID: 12639950Peripheral inner membrane protein facing the cytoplasmF1F1Cytoplasmic (Experimental)N/AyesyesNo180.326CYT0-0.200913-00Yn4-12c17/18oCytoplasmic9.9790.36
KDTA_ECOLIP0AC75
waaA

kdtA

kdtAb3633 JW3608 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.4.-.-) (KDO transferase)Potential membrane association Monotopic membrane protein (By similarity)Involved in lipopolysaccharide biosynthesis PMID: 2651435 PMID: 1577828. Embedded in the cytoplasmic face of the inner membrane, WaaA catalyzes the transfer of 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) to the lipid A precursor of LPS (in Aquifex aeolicus) PMID: 22474366.PMID: 1577828 PMID: 15866922 PMID: 22474366Integral Inner Membrane BBout/inCell inner membrane | Single-pass membrane proteinCytoplasmicIM (predicted)SRPSECyesyesNo640.121CYT0-0.200913-01="0"o6-23iUnknown215.12
KDSB_ECOLIP04951
kdsB
b0918 JW0901 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38) (CMP-2-keto-3-deoxyoctulosonic acid synthase) (CKS) (CMP-KDO synthase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo610.107CYT0-0.200913-00="0"iCytoplasmic10115.48
FABA_ECOLIP0A6Q3
fabA
b0954 JW0937 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.60) (Beta-hydroxydecanoyl thioester dehydrase)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo310.116CYT0-0.200913-00="0"iCytoplasmic1065.85
LEUC_ECOLIP0A6A6
leuC
b0072 JW0071 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) (Alpha-IPM isomerase) (IPMI) (Isopropylmalate isomerase) Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AnoyesNo410.123CYT0-0.200913-00="0"iCytoplasmic9.9770.18
LEUD_ECOLIP30126
leuD
b0071 JW0070 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) (Alpha-IPM isomerase) (IPMI) (Isopropylmalate isomerase)CytoplasmicAACytoplasmicIM (predicted)noyesNo180.111CYT0-0.200913-00="0"iCytoplasmic9.2692.5
LEU3_ECOLIP30125
leuB
b0073 JW5807 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (3-IPM-DH) (Beta-IPM dehydrogenase) (IMDH)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo220.115CYT0-0.200913-00="0"iCytoplasmic1058.18
SGBH_ECOLIP37678
sgbH

yiaQ

yiaQb3581 JW3553 3-keto-L-gulonate-6-phosphate decarboxylase sgbH (KGPDC) (EC 4.1.1.85) (3-dehydro-L-gulonate-6-phosphate decarboxylase)CytoplasmicAACytoplasmicN/AyesyesNo180.147CYT0-0.200913-00="0"iCytoplasmic8.9641.32
ULAD_ECOLIP39304
ulaD

sgaH yjfV

sgaH yjfVb4196 JW4154 3-keto-L-gulonate-6-phosphate decarboxylase ulaD (EC 4.1.1.85) (3-dehydro-L-gulonate-6-phosphate decarboxylase) (KGPDC) (L-ascorbate utilization protein D)CytoplasmicAACytoplasmicN/AyesyesNo470.112CYT0-0.200913-00="0"iCytoplasmic8.96
FADA_ECOLIP21151
fadA

oldA

oldAb3845 JW5578 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothiolase) (Fatty acid oxidation complex subunit beta)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo280.133CYT0-0.200913-00="0"iCytoplasmic9.9710.44
FADI_ECOLIP76503
fadI

yfcY

yfcYb2342 JW2339 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (ACSs) (Acetyl-CoA acyltransferase) (Acyl-CoA ligase) (Beta-ketothiolase) (Fatty acid oxidation complex subunit beta)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo320.192CYT0-0.200913-00="0"iCytoplasmic9.979.12
THTM_ECOLIP31142
sseA
b2521 JW2505 3-mercaptopyruvate sulfurtransferase (MST) (EC 2.8.1.2) (Rhodanese-like protein)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo100.108CYT0-0.200913-00="0"iUnknown5.41
PANB_ECOLIP31057
panB
b0134 JW0130 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) (Ketopantoate hydroxymethyltransferase) (KPHMT)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo350.122CYT0-0.200913-00="0"iCytoplasmic8.9643.82
UBID_ECOLIP0AAB4
ubiD

yigC yigY

yigC yigYb3843 JW3819 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-) (Polyprenyl p-hydroxybenzoate decarboxylase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane proteinCytoplasmicIM (predicted)yesyesNo650.107CYT0-0.200913-00="0"iCytoplasmic9.9737.33
UBIX_ECOLIP0AG03
ubiX

dedF

dedFb2311 JW2308 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-) (Polyprenyl p-hydroxybenzoate decarboxylase)CytoplasmicAACytoplasmicN/AyesyesNo600.127CYT0-0.200913-00Yn4-15c20/21oCytoplasmic9.2621.64
FABG_ECOLIP0AEK2
fabG
b1093 JW1079 3-oxoacyl-[acyl-carrier-protein] reductase (EC 1.1.1.100) (3-ketoacyl-acyl carrier protein reductase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo230.249CYT0-0.200913-00="0"iCytoplasmic9.2693.23
FABB_ECOLIP0A953
fabB

fabC

fabCb2323 JW2320 3-oxoacyl-[acyl-carrier-protein] synthase 1 (EC 2.3.1.41) (3-oxoacyl-[acyl-carrier-protein] synthase I) (Beta-ketoacyl-ACP synthase I) (KAS I)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo250.163CYT0-0.200913-00Yn4-15c24/25oCytoplasmic1013.39
FABF_ECOLIP0AAI5
fabF

fabJ

fabJb1095 JW1081 3-oxoacyl-[acyl-carrier-protein] synthase 2 (EC 2.3.1.179) (3-oxoacyl-[acyl-carrier-protein] synthase II) (Beta-ketoacyl-ACP synthase II) (KAS II)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo480.158CYT0-0.200913-00Yn6-14c20/21oCytoplasmic9.268.31
FABH_ECOLIP0A6R0
fabH
b1091 JW1077 3-oxoacyl-[acyl-carrier-protein] synthase 3 (EC 2.3.1.180) (3-oxoacyl-[acyl-carrier-protein] synthase III) (Beta-ketoacyl-ACP synthase III) (KAS III) (EcFabH)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo210.114CYT0-0.200913-00="0"iCytoplasmic1091.94
HCAB_ECOLIP0CI31
hcaB

phdD yfhX

phdD yfhXb2541 JW2525 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (EC 1.3.1.n1) (2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase) (CI-dihydrodiol dehydrogenase) (Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase) (Cis-3-(2-caCytoplasmicAACytoplasmicN/AnoyesNo340.119CYT0-0.200913-00="0"iCytoplasmic9.269.75
HCAC_ECOLIP0ABW0
hcaC

hcaA3 phdB yfhW

hcaA3 phdB yfhWb2540 JW2524 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit (Digoxigenin ferredoxin subunit)CytoplasmicAACytoplasmicN/AnoyesNo380.146CYT0-0.200913-00="0"iUnknown2112.99
HCAD_ECOLIP77650
hcaD

hcaA4 phdA yfhY

hcaA4 phdA yfhYb2542 JW2526 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component (EC 1.18.1.3) (Digoxigenin system ferredoxin--NAD(+) reductase component)CytoplasmicAACytoplasmicN/AnoyesNo290.119CYT0-0.200913-00="0"iCytoplasmic9.9740.87
HCAE_ECOLIP0ABR5
hcaE

digA hcaA hcaA1 phdC1 yfhU

digA hcaA hcaA1 phdC1 yfhUb2538 JW2522 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha (EC 1.14.12.19) (Digoxigenin subunit alpha)CytoplasmicAACytoplasmicN/AnoyesNo480.107CYT0-0.200913-00="0"iCytoplasmic9.9713.78
HCAF_ECOLIQ47140
hcaF

digB hcaA2 hcaB phdC2 yfhV

digB hcaA2 hcaB phdC2 yfhVb2539 JW2523 3-phenylpropionate/cinnamic acid dioxygenase subunit beta (EC 1.14.12.19) (Digoxigenin subunit beta)CytoplasmicAACytoplasmicN/AnoyesNo510.125CYT0-0.200913-00="0"iCytoplasmic9.266.28
AROA_ECOLIP0A6D3
aroA
b0908 JW0891 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo380.15CYT0-0.200913-00="0"iCytoplasmic8.9661.27
RS1_ECOLIP0AG67
rpsA

ssyF

ssyFb0911 JW0894 30S ribosomal protein S1Cytoplasmic (Experimental)PMID: 7041110 PMID: 7783627 PMID: 9600841 PMID: 11248028RibosomalrrCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo310.131CYT0-0.200913-00="0"iCytoplasmic1086.64
RS10_ECOLIP0A7R5
rpsJ

nusE

nusEb3321 JW3283 30S ribosomal protein S10RibosomalrrCytoplasmicN/AyesyesNo240.151CYT0-0.200913-00="0"iCytoplasmic9.97
RS11_ECOLIP0A7R9
rpsK
b3297 JW3259 30S ribosomal protein S11RibosomalrrCytoplasmicN/AyesyesNo480.109CYT0-0.200913-00="0"iCytoplasmic9.2680.92
RS12_ECOLIP0A7S3
rpsL

strA

strAb3342 JW3304 30S ribosomal protein S12 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544PMID: 21541338RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo270.105CYT0-0.200913-00="0"iCytoplasmic1084.22
RS13_ECOLIP0A7S9
rpsM
b3298 JW3260 30S ribosomal protein S13RibosomalrrCytoplasmicN/AyesyesNo330.108CYT0-0.200913-00="0"iCytoplasmic9.9798.64
RS14_ECOLIP0AG59
rpsN
b3307 JW3269 30S ribosomal protein S14RibosomalrrCytoplasmicN/AyesyesNo370.154CYT0-0.200913-00="0"iCytoplasmic9.26111.76
RS15_ECOLIP0ADZ4
rpsO

secC

secCb3165 JW3134 30S ribosomal protein S15RibosomalrrCytoplasmicN/AyesyesNo100.137CYT0-0.200913-00="0"iCytoplasmic9.26128.68
RS16_ECOLIP0A7T3
rpsP
b2609 JW2590 30S ribosomal protein S16RibosomalrrCytoplasmicN/AyesyesNo230.105CYT0-0.200913-00="0"iCytoplasmic9.26108.94
RS17_ECOLIP0AG63
rpsQ

neaA

neaAb3311 JW3273 30S ribosomal protein S17Experimentally verified gene-product. Methionine start codon is cleaved.RibosomalrrCytoplasmicN/AyesyesNo360.119CYT0-0.200913-00="0"iCytoplasmic9.9783.23
RS18_ECOLIP0A7T7
rpsR
b4202 JW4160 30S ribosomal protein S18RibosomalrrCytoplasmicN/AnoyesNo600.102CYT0-0.200913-00="0"iCytoplasmic9.2663.82
RS19_ECOLIP0A7U3
rpsS
b3316 JW3278 30S ribosomal protein S19RibosomalrrCytoplasmicN/AnoyesNo230.114CYT0-0.200913-00="0"iCytoplasmic9.2691.48
RS2_ECOLIP0A7V0
rpsB
b0169 JW0164 30S ribosomal protein S2Translation unitRibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo180.121CYT0-0.200913-00="0"iCytoplasmic1076.54
RS20_ECOLIP0A7U7
rpsT
b0023 JW0022 30S ribosomal protein S20RibosomalrrCytoplasmicN/AnoyesNo380.179CYT0-0.200913-00="0"iCytoplasmic9.26102.96
RS21_ECOLIP68679
rpsU
b3065 JW3037 30S ribosomal protein S21RibosomalrrCytoplasmicN/AnoyesNo270.102CYT0-0.200913-00="0"iCytoplasmic9.26100.26
RS3_ECOLIP0A7V3
rpsC
b3314 JW3276 30S ribosomal protein S3RibosomalrrCytoplasmicN/AnoyesNo250.137CYT0-0.200913-00="0"iCytoplasmic1075.11
RS4_ECOLIP0A7V8
rpsD

ramA

ramAb3296 JW3258 30S ribosomal protein S4Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AyesyesNo280.125CYT0-0.200913-00="0"iCytoplasmic9.97
RS5_ECOLIP0A7W1
rpsE

spc

spcb3303 JW3265 30S ribosomal protein S5Translation unitRibosomalrrCytoplasmicN/AyesyesNo380.112CYT0-0.200913-00="0"iCytoplasmic9.97
RS6_ECOLIP02358
rpsF
b4200 JW4158 30S ribosomal protein S6 [Cleaved into: 30S ribosomal protein S6, fully modified isoform; 30S ribosomal protein S6, non-modified isoform]Ribosome Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AyesyesNo630.103CYT0-0.200913-00="0"iCytoplasmic1098.05
RS7_ECOLIP02359
rpsG
b3341 JW3303 30S ribosomal protein S7RibosomalrrCytoplasmicN/AyesyesNo400.11CYT0-0.200913-00="0"iCytoplasmic9.2694.44
RS8_ECOLIP0A7W7
rpsH
b3306 JW3268 30S ribosomal protein S8TranslationRibosomalrrCytoplasmicN/AyesyesNo200.135CYT0-0.200913-00="0"iCytoplasmic9.9785.97
RS9_ECOLIP0A7X3
rpsI
b3230 JW3199 30S ribosomal protein S9RibosomalrrCytoplasmicN/AyesyesNo30.103CYT0-0.200913-00="0"iCytoplasmic9.97113.14
HSLO_ECOLIP0A6Y5
hslO

yrfI

yrfIb3401 JW5692 33 kDa chaperonin (Heat shock protein 33) (HSP33)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (230 232;263 266) PMID: 10764757PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicIM (predicted)yesyesNo630.125CYT0-0.200913-00="0"iCytoplasmic1088.87
ACPT_ECOLIP37623
acpT

yhhU

yhhUb3475 JW3440 4'-phosphopantetheinyl transferase AcpT (EC 2.7.8.-)CytoplasmicAACytoplasmicN/AyesyesNo630.119CYT0-0.200913-00="0"iUnknown213.91
ENTD_ECOLIP19925
entD
b0583 JW5085 4'-phosphopantetheinyl transferase entD (EC 2.7.8.-) (Enterobactin synthase component D) (Enterochelin synthase D)Peripheral inner membrane protein facing the cytoplasm (Experimental)Posttranslationally modifies both EntB/G and EntF. Posttranslationally modifies both EntB/G and EntFPMID: 2526281CytoplasmicAAMembrane anchoredN/AyesyesNo380.136CYT0-0.200913-00="0"iCytoplasmic8.9636.31
MALQ_ECOLIP15977
malQ

malA

malAb3416 JW3379 4-alpha-glucanotransferase (EC 2.4.1.25) (Amylomaltase) (Disproportionating enzyme) (D-enzyme)Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo230.214SpI220.437923-00="0"iCytoplasmic1031.98
WECF_ECOLIP56258
wecF

rffT yifM

rffT yifMb4481 JW5596 4-alpha-L-fucosyltransferase (EC 2.4.1.-) (TDP-Fuc4NAc:lipid II Fuc4NAc transferase) (Fuc4NAc transferase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1in/inCell inner membrane | Peripheral membrane proteinCytoplasmicIM (predicted)pseudogene (Ochman & Davalos, 2006)yesyesNo290.203CYT0-0.200913-00="0"iUnknown248.35
GABT_ECOLIP22256
gabT
b2662 JW2637 4-aminobutyrate aminotransferase (EC 2.6.1.19) ((S)-3-amino-2-methylpropionate transaminase) (EC 2.6.1.22) (GABA aminotransferase) (GABA-AT) (Gamma-amino-N-butyrate transaminase) (GABA transaminase) (Glutamate:succinic semialdehyde transaminase) (L-AIBAT)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo650.16CYT0-0.200913-00="0"iCytoplasmic9.9777.26
PUUE_ECOLIP50457
puuE

goaG

goaGb1302 JW1295 4-aminobutyrate aminotransferase (EC 2.6.1.19) (GABA aminotransferase) (GABA-AT) (Gamma-amino-N-butyrate transaminase) (GABA transaminase) (Glutamate:succinic semialdehyde transaminase)CytoplasmicAACytoplasmicN/AyesyesNo290.166CYT0-0.200913-00="0"iCytoplasmic9.9742.41
KDUI_ECOLIQ46938
kduI

yqeE

yqeEb2843 JW2811 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17) (5-keto-4-deoxyuronate isomerase) (DKI isomerase)CytoplasmicAACytoplasmicN/AyesyesNo130.123CYT0-0.200913-00="0"iCytoplasmic8.9663.24
ISPE_ECOLIP62615
ispE

ipk ychB

ipk ychBb1208 JW1199 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK) (EC 2.7.1.148) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase)CytoplasmicAACytoplasmicN/AyesyesNo230.205CYT0-0.200913-00="0"iCytoplasmic8.9699.89
HOA_ECOLIP51020
mhpE
b0352 JW0343 4-hydroxy-2-oxovalerate aldolase (HOA) (EC 4.1.3.39) (4-hydroxy-2-keto-pentanoic acid aldolase) (4-hydroxy-2-oxopentanoate aldolase)CytoplasmicAACytoplasmicN/AnoyesNo500.105CYT0-0.200913-00="0"iCytoplasmic109.55
ISPG_ECOLIP62620
ispG

gcpE

gcpEb2515 JW2499 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (EC 1.17.7.1) (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase) (Protein gcpE) (Protein E)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo440.118CYT0-0.200913-00="0"iCytoplasmic9.97
ISPH_ECOLIP62623
ispH

lytB yaaE

lytB yaaEb0029 JW0027 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)CytoplasmicAACytoplasmicN/AyesyesNo280.217CYT0-0.200913-00="0"iCytoplasmic9.9775.45
UBIA_ECOLIP0AGK1
ubiA

cyr

cyrb4040 JW4000 4-hydroxybenzoate octaprenyltransferase (EC 2.5.1.-) (4-HB polyprenyltransferase)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECyesyesNo360.118TMH06.00125-77="0"i23-40o46-70i99-132o152-182i210-229o235-252i273-289oCytoplasmicMembrane10
PDXA_ECOLIP19624
pdxA
b0052 JW0051 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262) (4-(phosphohydroxy)-L-threonine dehydrogenase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo660.214CYT0-0.200913-00="0"iCytoplasmic9.9794.55
MTNN_ECOLIP0AF12
mtnN

mtn pfs yadA

mtn pfs yadAb0159 JW0155 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase) (MTAN) (EC 3.2.2.9) (5'-methylthioadenosine nucleosidase) (MTA nucleosidase) (P46) (S-adenosylhomocysteine nucleosidase) (AdoHcy nucleosidase) (SAH nucleosidase) (SRH nucleoCytoplasmicAACytoplasmicN/AyesyesNo560.13CYT0-0.200913-00="0"iCytoplasmic8.96106.46
5DNU_ECOLIP76491
yfbR
b2291 JW2288 5'-nucleotidase yfbR (EC 3.1.3.5) (5'-deoxyribonucleotidase) (Nucleoside 5'-monophosphate phosphohydrolase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo460.185CYT0-0.200913-00="0"iCytoplasmic8.9667.69
YJJG_ECOLIP0A8Y1
yjjG
b4374 JW4336 5'-nucleotidase yjjG (EC 3.1.3.5) (Nucleoside 5'-monophosphate phosphohydrolase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo370.136CYT0-0.200913-00="0"iCytoplasmicMembrane8.4652.25
METF_ECOLIP0AEZ1
metF
b3941 JW3913 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)CytoplasmicAACytoplasmicN/AyesyesNo180.111CYT0-0.200913-00="0"iCytoplasmic9.9725.78
HIUH_ECOLIP76341
hiuH

yedX

yedXb1970 JW1953 5-hydroxyisourate hydrolase (HIU hydrolase) (HIUHase) (EC 3.5.2.17) (Transthyretin-like protein) (TLP) (Transthyretin-related protein) (TRP)Periplasm (Probable)PMID: 12542701PeriplasmicGGSecretedPeriplasmicN/ASECyesyesYes240.79SpI2318.1944-00Yn6-17c23/24oUnknown2.578.26
GARL_ECOLIP23522
garL

yhaF

yhaFb3126 JW3095 5-keto-4-deoxy-D-glucarate aldolase (KDGluc aldolase) (KDGlucA) (EC 4.1.2.20) (2-dehydro-3-deoxy-D-glucarate aldolase) (2-keto-3-deoxy-D-glucarate aldolase) (5-dehydro-4-deoxy-D-glucarate aldolase) (Alpha-keto-beta-deoxy-D-glucarate aldolase)CytoplasmicAACytoplasmicN/AyesyesNo290.122CYT0-0.200913-00="0"iCytoplasmic9.26
MCRA_ECOLIP24200
mcrA

rglA

rglAb1159 JW1145 5-methylcytosine-specific restriction enzyme A (EC 3.1.21.-) (EcoKMcrA)CytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo390.103CYT0-0.200913-00="0"iCytoplasmic8.9639.62
MCRB_ECOLIP15005
mcrB

rglB

rglBb4346 JW5871 5-methylcytosine-specific restriction enzyme B (EC 3.1.21.-) (EcoKMcrBC)Nucleoid (Experimental) Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities. 26494802649480NucleoidNNCytoplasmicN/AyesyesNo520.179CYT0-0.200913-00="0"iUnknown242.77
METE_ECOLIP25665
metE
b3829 JW3805 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) (Cobalamin-independent methionine synthase) (Methionine synthase, vitamin-B12 independent isozyme)CytoplasmicAACytoplasmicN/AyesyesNo230.107CYT0-0.200913-00="0"iCytoplasmic8.9633.45
RL1_ECOLIP0A7L0
rplA
b3984 JW3947 50S ribosomal protein L1Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo370.122CYT0-0.200913-00="0"iCytoplasmic9.97
RL10_ECOLIP0A7J3
rplJ
b3985 JW3948 50S ribosomal protein L10 (50S ribosomal protein L8)Inner membrane (Experimental)TranslationPMID: 15919657RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo250.154CYT0-0.200913-00="0"iCytoplasmic9.2674.56
RL11_ECOLIP0A7J7
rplK

relC

relCb3983 JW3946 50S ribosomal protein L11 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo270.18CYT0-0.200913-00="0"iCytoplasmic9.26104.93
RL13_ECOLIP0AA10
rplM
b3231 JW3200 50S ribosomal protein L13 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo300.125CYT0-0.200913-00="0"iCytoplasmic9.9776.25
RL14_ECOLIP0ADY3
rplN
b3310 JW3272 50S ribosomal protein L14 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo340.256CYT0-0.200913-00="0"iCytoplasmic9.2671.04
RL15_ECOLIP02413
rplO
b3301 JW3263 50S ribosomal protein L15Inner membrane (Experimental)Ribosomal.PMID: 15919657RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo670.101CYT0-0.200913-00="0"iCytoplasmic9.9764.26
RL16_ECOLIP0ADY7
rplP
b3313 JW3275 50S ribosomal protein L16RibosomalrrCytoplasmicN/AyesyesNo360.141CYT0-0.200913-00="0"iCytoplasmic9.9789.5
RL17_ECOLIP0AG44
rplQ
b3294 JW3256 50S ribosomal protein L17Inner membrane (Experimental)PMID: 15919657RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo420.113CYT0-0.200913-00="0"iCytoplasmic9.9776.67
RL18_ECOLIP0C018
rplR
b3304 JW3266 50S ribosomal protein L18RibosomalrrCytoplasmicN/AyesyesNo600.124CYT0-0.200913-00="0"iCytoplasmic9.97
RL19_ECOLIP0A7K6
rplS
b2606 JW2587 50S ribosomal protein L19 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AyesyesNo430.107CYT0-0.200913-00="0"iCytoplasmic9.97101.49
RL2_ECOLIP60422
rplB
b3317 JW3279 50S ribosomal protein L2Translation unitRibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo320.106CYT0-0.200913-00="0"iCytoplasmic9.9799.75
RL20_ECOLIP0A7L3
rplT

pdzA

pdzAb1716 JW1706 50S ribosomal protein L20RibosomalrrCytoplasmicN/AyesyesNo470.112CYT0-0.200913-00="0"iCytoplasmic9.2699.59
RL21_ECOLIP0AG48
rplU
b3186 JW3153 50S ribosomal protein L21 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo370.108CYT0-0.200913-00="0"iCytoplasmic9.9755.9
RL22_ECOLIP61175
rplV

eryB

eryBb3315 JW3277 50S ribosomal protein L22RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo590.241CYT0-0.200913-00="0"iCytoplasmic9.9782.3
RL23_ECOLIP0ADZ0
rplW
b3318 JW3280 50S ribosomal protein L23RibosomalrrCytoplasmicN/AnoyesNo230.116CYT0-0.200913-00="0"iCytoplasmic9.2670.05
RL24_ECOLIP60624
rplX
b3309 JW3271 50S ribosomal protein L24 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AyesyesNo540.102CYT0-0.200913-00="0"iCytoplasmic9.26111.8
RL25_ECOLIP68919
rplY
b2185 JW2173 50S ribosomal protein L25RibosomalrrCytoplasmicN/AnoyesNo550.112CYT0-0.200913-00="0"iCytoplasmic9.9786.3
RL27_ECOLIP0A7L8
rpmA
b3185 JW3152 50S ribosomal protein L27 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo640.126CYT0-0.200913-00="0"iCytoplasmic9.2634.33
RL28_ECOLIP0A7M2
rpmB
b3637 JW3612 50S ribosomal protein L28RibosomalrrCytoplasmicN/AnoyesNo540.114CYT0-0.200913-00="0"iCytoplasmic9.2654.57
RL29_ECOLIP0A7M6
rpmC
b3312 JW3274 50S ribosomal protein L29RibosomalrrCytoplasmicN/AnoyesNo330.119CYT0-0.200913-00="0"iCytoplasmic9.26
RL3_ECOLIP60438
rplC
b3320 JW3282 50S ribosomal protein L3Translation unit Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AyesyesNo270.111CYT0-0.200913-00="0"iCytoplasmic9.97
RL30_ECOLIP0AG51
rpmD
b3302 JW3264 50S ribosomal protein L30 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo360.114CYT0-0.200913-00="0"iCytoplasmic9.26100.94
RL31_ECOLIP0A7M9
rpmE
b3936 JW3907 50S ribosomal protein L31 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544PMID: 21541338RibosomalrrCytoplasmicN/AyesyesNo310.105CYT0-0.200913-00="0"iCytoplasmic9.2666.19
RL31B_ECOLIP0A7N1
rpmE2

ykgM

ykgMb0296 JW5035 50S ribosomal protein L31 type BCytoplasmicAACytoplasmicN/AyesyesNo560.107CYT0-0.200913-00="0"iCytoplasmic9.2671.7
RL32_ECOLIP0A7N4
rpmF
b1089 JW1075 50S ribosomal protein L32RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo240.104CYT0-0.200913-00="0"iCytoplasmic9.26
RL33_ECOLIP0A7N9
rpmG
b3636 JW3611 50S ribosomal protein L33 Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo250.103CYT0-0.200913-00="0"iCytoplasmic9.26
RL34_ECOLIP0A7P5
rpmH

rimA ssaF

rimA ssaFb3703 JW3680 50S ribosomal protein L34Small membrane protein detected experimentally Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicN/AnoyesNo230.103CYT0-0.200913-00="0"iCytoplasmic9.2690.43
RL35_ECOLIP0A7Q1
rpmI
b1717 JW1707 50S ribosomal protein L35 (Ribosomal protein A)RibosomalrrCytoplasmicN/AnoyesNo620.1CYT0-0.200913-00="0"iCytoplasmic9.26121.89
RL36_ECOLIP0A7Q6
rpmJ
b3299 JW3261 50S ribosomal protein L36 (Ribosomal protein B)Small membrane protein detected experimentallyRibosomalrrCytoplasmicN/AnoyesNo350.106CYT0-0.200913-00="0"iCytoplasmic9.26
RL362_ECOLIQ2EEQ2
rpmJ2

ykgO

ykgOb4506 JW5034 50S ribosomal protein L36 2Translation, Ribosomal. Expressed during stationary phase in minimal medium (at protein level). Disulfide bond-containing protein PMID: 25664544Ribosomalrr-N/AnoyesNo380.12CYT0-0.200913-00="0"iUnknown2136.42
RL4_ECOLIP60723
rplD

eryA

eryAb3319 JW3281 50S ribosomal protein L4Translation unit. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 21541338RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo370.154CYT0-0.200913-00="0"iCytoplasmic9.26
RL5_ECOLIP62399
rplE
b3308 JW3270 50S ribosomal protein L5RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo460.11CYT0-0.200913-00="0"iCytoplasmic9.97
RL6_ECOLIP0AG55
rplF
b3305 JW3267 50S ribosomal protein L6RibosomalrrCytoplasmicN/AyesyesNo470.109CYT0-0.200913-00="0"iCytoplasmic9.26
RL7_ECOLIP0A7K2
rplL
b3986 JW3949 50S ribosomal protein L7/L12 (L8)Experimentally verified gene-product. Methionine start codon is cleaved.RibosomalrrCytoplasmicN/AnoyesNo490.715CYT0-0.200913-00="0"iUnknown4.9998.51
RL9_ECOLIP0A7R1
rplI
b4203 JW4161 50S ribosomal protein L9Inner membrane (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 15919657 PMID: 21541338RibosomalrrCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo240.144CYT0-0.200913-00="0"iCytoplasmic1084.07
RISB_ECOLIP61714
ribH

ribH ybaF

ribH ybaFb0415 JW0405 6,7-dimethyl-8-ribityllumazine synthase (DMRL synthase) (Lumazine synthase) (EC 2.5.1.9) (Riboflavin synthase beta chain)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo690.129CYT0-0.200913-00="0"iCytoplasmic9.26
QUED_ECOLIP65870
queD

ygcM

ygcMb2765 JW2735 6-carboxy-5,6,7,8-tetrahydropterin synthase (CPH4 synthase) (EC 4.-.-.-) (Queuosine biosynthesis protein queD)CytoplasmicAACytoplasmicN/AyesyesNo180.109CYT0-0.200913-00="0"iCytoplasmic9.2690.89
CHBF_ECOLIP17411
chbF

celF ydjD

celF ydjDb1734 JW1723 6-phospho-beta-glucosidase (EC 3.2.1.86) (Cellobiose-6-phosphate hydrolase) (Phospho-chitobiase)CytoplasmicAACytoplasmicN/AyesyesNo630.113CYT0-0.200913-00="0"iCytoplasmic8.9624.02
ASCB_ECOLIP24240
ascB
b2716 JW2686 6-phospho-beta-glucosidase AscB (EC 3.2.1.86)CytoplasmicAACytoplasmicN/AyesyesNo160.219CYT0-0.200913-00="0"iCytoplasmic9.2628.46
BGLA_ECOLIQ46829
bglA

bglD yqfC

bglD yqfCb2901 JW2869 6-phospho-beta-glucosidase BglA (EC 3.2.1.86)Trigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo200.217CYT0-0.200913-00="0"iCytoplasmic9.9769.73
BGLB_ECOLIP11988
bglB
b3721 JW3699 6-phospho-beta-glucosidase BglB (EC 3.2.1.86)CytoplasmicAACytoplasmicN/AyesyesNo160.139CYT0-0.200913-00="0"iCytoplasmic9.2619.33
K6PF1_ECOLIP0A796
pfkA
b3916 JW3887 6-phosphofructokinase isozyme 1 (EC 2.7.1.11) (6-phosphofructokinase isozyme I) (Phosphofructokinase 1) (Phosphohexokinase 1)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo200.426CYT0-0.200913-00="0"iCytoplasmic1076.65
K6PF2_ECOLIP06999
pfkB
b1723 JW5280 6-phosphofructokinase isozyme 2 (Phosphofructokinase-2) (EC 2.7.1.11)CytoplasmicAACytoplasmicN/AyesyesNo580.118CYT0-0.200913-00="0"iCytoplasmic9.9783.6
6PGD_ECOLIP00350
gnd
b2029 JW2011 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo200.182CYT0-0.200913-00="0"iCytoplasmic8.9690.25
RCNR_ECOLIP64530
rcnR

yohL

yohLb2105 JW2092 6-phosphogluconolactonase (6-P-gluconolactonase) (Pgl) (EC 3.1.1.31)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo370.161CYT0-0.200913-00="0"iCytoplasmic8.96101.47
CH60_ECOLIP0A6F5
groL

groEL mopA

groEL mopAb4143 JW4103 60 kDa chaperonin (Protein Cpn60) (groEL protein)Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo580.12CYT0-0.200913-00="0"iCytoplasmic1094.55
HDHA_ECOLIP0AET8
hdhA

hsdH

hsdHb1619 JW1611 7-alpha-hydroxysteroid dehydrogenase (7-alpha-HSDH) (EC 1.1.1.159) Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AyesyesNo480.2CYT0-0.200913-00="0"iCytoplasmic9.2668.03
QUEE_ECOLIP64554
queE

ygcF

ygcFb2777 JW2748 7-carboxy-7-deazaguanine synthase homologOld gene name YgaW.CytoplasmicAACytoplasmicN/AyesyesNo390.13CYT0-0.200913-00="0"iCytoplasmic9.9763.44
QUEC_ECOLIP77756
queC

ybaX

ybaXb0444 JW0434 7-cyano-7-deazaguanine synthase (EC 6.3.-.-) (7-cyano-7-carbaguanine synthase) (PreQ(0) synthase) (Queuosine biosynthesis protein queC)CytoplasmicAACytoplasmicIM (predicted)yesyesNo220.126CYT0-0.200913-00="0"iCytoplasmic8.96104.89
BIOF_ECOLIP12998
bioF
b0776 JW0759 8-amino-7-oxononanoate synthase (AONS) (EC 2.3.1.47) (7-keto-8-amino-pelargonic acid synthase) (7-KAP synthase) (KAPA synthase) (8-amino-7-ketopelargonate synthase) (L-alanine--pimeloyl-CoA ligase)CytoplasmicAACytoplasmicN/AyesyesNo300.112CYT0-0.200913-00="0"iCytoplasmic9.263.2
YBCO_ECOLIP68661
ybcO
b0549 JW0537 82 prophage-derived uncharacterized protein ybcOCytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo320.12CYT0-0.200913-00="0"iCytoplasmic8.9697.85
MUTY_ECOLIP17802
mutY

micA

micAb2961 JW2928 A/G-specific adenine glycosylase (EC 3.2.2.-)CytoplasmicAACytoplasmicN/AyesyesNo580.111CYT0-0.200913-00="0"iCytoplasmic9.2631.84
ARTJ_ECOLIP30860
artJ
b0860 JW0844 ABC transporter arginine-binding protein 1Periplasm (Probable) Periplasmic (Experimental) (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 8801423 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350PMID: 8801422 PMID: 8801423 PMID: 22030350PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECDsbAyesyesYes200.849SpI1918.9337-00Yn4-15c19/20oPeriplasmic1073.37
UUP_ECOLIP43672
uup

ycbH ycbI

ycbH ycbIb0949 JW0932 ABC transporter ATP-binding protein uupPeripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.126CYT0-0.200913-00="0"iCytoplasmic10
YOJI_ECOLIP33941
yojI

yojJ

yojJb2211 JW2199 ABC transporter ATP-binding protein yojIIntegral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo300.136TMH012.5457-66="0"i12-36o56-80i125-144o150-169i231-253o273-296iCytoplasmicMembrane1018.16
YPHF_ECOLIP77269
yphF
b2548 JW2532 ABC transporter periplasmic-binding protein yphFPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes270.817SpI2620.8859-00Yn11-22c26/27oPeriplasmic9.7663.54
YTFQ_ECOLIP39325
ytfQ
b4227 JW4186 ABC transporter periplasmic-binding protein ytfQPeriplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECDsbAyesyesYes220.849SpI2121.6548-10Yn5-13c21/22oPeriplasmic1090.28
ACDH_ECOLIP77580
mhpF

mhpE

mhpEb0351 JW0342 Acetaldehyde dehydrogenase (EC 1.2.1.10) (Acetaldehyde dehydrogenase [acetylating])CytoplasmicAACytoplasmicN/AnoyesNo700.129CYT0-0.200913-00="0"iCytoplasmic8.9658.13
ATOD_ECOLIP76458
atoD
b2221 JW2215 Acetate CoA-transferase subunit alpha (EC 2.8.3.8) (Acetyl-CoA:acetoacetate-CoA transferase subunit alpha)CytoplasmicAACytoplasmicN/AnoyesNo390.125TMH00.259434-00="0"iCytoplasmic9.9754.47
ATOA_ECOLIP76459
atoA
b2222 JW2216 Acetate CoA-transferase subunit beta (EC 2.8.3.8) (Acetyl-CoA:acetoacetate-CoA transferase subunit beta)CytoplasmicAACytoplasmicN/AnoyesNo610.12CYT0-0.200913-00="0"iCytoplasmic9.9725.77
ACKA_ECOLIP0A6A3
ackA

ack

ackb2296 JW2293 Acetate kinase (EC 2.7.2.1) (Acetokinase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo200.328CYT0-0.200913-00="0"iCytoplasmic10112.72
ICLR_ECOLIP16528
iclR
b4018 JW3978 Acetate operon repressorNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AyesyesNo460.149CYT0-0.200913-00="0"iCytoplasmic9.2620.61
ATOC_ECOLIQ06065
atoC

az

azb2220 JW2214 Acetoacetate metabolism regulatory protein AtoC (Ornithine decarboxylase antizyme) (Ornithine/arginine decarboxylase inhibitor)Nucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasm.CytoplasmicN/AyesyesNo350.155CYT0-0.200913-00="0"iCytoplasmic1039.49
ILVB_ECOLIP08142
ilvB
b3671 JW3646 Acetolactate synthase isozyme 1 large subunit (AHAS-I) (EC 2.2.1.6) (Acetohydroxy-acid synthase I large subunit) (ALS-I) Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AyesyesNo450.118CYT0-0.200913-00="0"iCytoplasmic9.9729.18
ILVN_ECOLIP0ADF8
ilvN
b3670 JW3645 Acetolactate synthase isozyme 1 small subunit (EC 2.2.1.6) (Acetohydroxy-acid synthase I small subunit) (AHAS-I) (ALS-I)CytoplasmicAACytoplasmicN/AnoyesNo360.123CYT0-0.200913-00="0"iCytoplasmic9.2699.18
ILVG_ECOLIP00892
ilvG
b4488 b3767Acetolactate synthase isozyme 2 large subunit (AHAS-II) (EC 2.2.1.6) (ALS-II) (Acetohydroxy-acid synthase II large subunit)CytoplasmicAACytoplasmicN/Apseudogene (Ochman & Davalos, 2006)yesyesNo220.189CYT0-0.200913-00="0"iCytoplasmic9.9716.73
ILVM_ECOLIP0ADG1
ilvM
b3769 JW3742 Acetolactate synthase isozyme 2 small subunit (EC 2.2.1.6) (ALS-II) (Acetohydroxy-acid synthase II small subunit) (AHAS-II)CytoplasmicAACytoplasmicN/AnoyesNo430.131CYT0-0.200913-00="0"iUnknown257.64
ILVI_ECOLIP00893
ilvI
b0077 JW0076 Acetolactate synthase isozyme 3 large subunit (EC 2.2.1.6) (AHAS-III) (ALS-III) (Acetohydroxy-acid synthase III large subunit)CytoplasmicAACytoplasmicN/AyesyesNo250.127CYT0-0.200913-00="0"iCytoplasmic9.973.22
ILVH_ECOLIP00894
ilvH

brnP

brnPb0078 JW0077 Acetolactate synthase isozyme 3 small subunit (EC 2.2.1.6) (ALS-III) (Acetohydroxy-acid synthase III small subunit) (AHAS-III)CytoplasmicAACytoplasmicN/AnoyesNo180.108CYT0-0.200913-00Yn4-9c17/18oCytoplasmic9.9715.13
AES_ECOLIP23872
aes

ybaC

ybaCb0476 JW0465 Acetyl esterase (EC 3.1.1.-) (EcE)Cytoplasm (Probable)PMID: 9576853CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo340.108CYT0-0.200913-00="0"iCytoplasmic1034.4
ATOB_ECOLIP76461
atoB
b2224 JW2218 Acetyl-CoA acetyltransferase (EC 2.3.1.9) (Acetoacetyl-CoA thiolase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo230.375CYT0-0.200913-00="0"iCytoplasmic9.978.53
ACCA_ECOLIP0ABD5
accA
b0185 JW0180 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (ACCase subunit alpha) (Acetyl-CoA carboxylase carboxyltransferase subunit alpha) (EC 6.4.1.2)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.103CYT0-0.200913-00="0"iCytoplasmic1028.79
ACCD_ECOLIP0A9Q5
accD

dedB usg

dedB usgb2316 JW2313 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (ACCase subunit beta) (Acetyl-CoA carboxylase carboxyltransferase subunit beta) (EC 6.4.1.2)Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation related.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo180.112CYT0-0.200913-00="0"iCytoplasmic9.9762.49
ACSA_ECOLIP27550
acs

yfaC

yfaCb4069 JW4030 Acetyl-coenzyme A synthetase (EC 6.2.1.1) (Acetate--CoA ligase) (Acyl-activating enzyme)Peripheral inner membrane protein facing the cytoplasm (By similarity) Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous to FadD13 protein of Mycobacterium that is peripheral. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 22560731 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo270.136CYT0-0.200913-00="0"iCytoplasmic9.9762.33
ARGB_ECOLIP0A6C8
argB
b3959 JW5553 Acetylglutamate kinase (EC 2.7.2.8) (N-acetyl-L-glutamate 5-phosphotransferase) (NAG kinase) (AGK)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo220.115CYT0-0.200913-00="0"iCytoplasmic9.2681.14
ARGE_ECOLIP23908
argE
b3957 JW3929 Acetylornithine deacetylase (AO) (Acetylornithinase) (EC 3.5.1.16) (N-acetylornithinase) (NAO)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo230.163CYT0-0.200913-00="0"iCytoplasmic9.9742.16
ARGD_ECOLIP18335
argD

dapC dtu

dapC dtub3359 JW3322 Acetylornithine/succinyldiaminopimelate aminotransferase (ACOAT) (DapATase) (Succinyldiaminopimelate transferase) (EC 2.6.1.11) (EC 2.6.1.17)Copurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo250.129CYT0-0.200913-00="0"iCytoplasmic9.9729.13
YPEA_ECOLIP76539
ypeA
b2434 JW2427 Acetyltransferase ypeA (EC 2.3.1.-)CytoplasmicAACytoplasmicN/AyesyesNo530.103CYT0-0.200913-00="0"iCytoplasmic8.9680.41
ASR_ECOLIP36560
asr
b1597 JW5826 Acid shock proteinPeriplasmic (Experimental)PMID: 12670971PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes220.907SpI2128.9272-00Yn4-15c21/22oPeriplasmic10
MSYB_ECOLIP25738
msyB
b1051 JW1039 Acidic protein msyBCytoplasmicAACytoplasmicN/AyesyesNo560.112CYT0-0.200913-00="0"iCytoplasmic8.9691.33
ACON1_ECOLIP25516
acnA

acn

acnb1276 JW1268 Aconitate hydratase 1 (Aconitase 1) (EC 4.2.1.3) (Citrate hydro-lyase 1)Cytoplasmic (Experimental)PMID: 10585860CytoplasmicAACytoplasmic (Experimental)N/AnoyesNo470.106CYT0-0.200913-00="0"iCytoplasmic9.9740.75
ACNB_ECOLIP36683
acnB

yacI yacJ

yacI yacJb0118 JW0114 Aconitate hydratase 2 (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)Trigger factor-sensitive aggregation. PMID: 10585860 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10585860Peripheral inner membrane protein facing the cytoplasmF1F1Cytoplasmic (Experimental)N/ATFyesyesNo420.124CYT0-0.200913-00="0"iCytoplasmic9.9790.34
ACRA_ECOLIP0AE06
acrA

lir mtcA

lir mtcAb0463 JW0452 Acriflavine resistance protein ALipoprotein (Experimental) Inner membrane (Probable) Cell inner membrane (Experimental) Inner membrane lipoprotein (Experimental)Proven signal peptidase II substrate. PMID: 10894736PMID: 15174130 PMID: 15228545 PMID: 16079137 PMID: 10894736Inner Membrane LipoproteinEECell inner membrane | Lipid-anchorInner membrane lipoprotein (Experimental)Soluble protein detected in Inner Membrane FractionSECLOL: Localization Of LipoproteinsyesyesYes220.194SpII2419.636Pos+2=D00Yn6-16c21/22oCytoplasmicMembrane1064.7
ACRB_ECOLIP31224
acrB

acrE

acrEb0462 JW0451 Acriflavine resistance protein B (Probable) Alpha-helcal transmembrane protein (Experimental) Integral inner membrane (Probable) Cell inner membrane (Probable) Integral inner membrane (Experimental)AcrB is a major multidrug exporter in Escherichia coli. It cooperates with a membrane fusion protein, AcrA, and an outer membrane channel, TolC (AcrAB-TolC multidrug efflux system) PMID: 15228545. Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertionPMID: 9008159. Exhibites aberrant localization by microscopy in the absence of YidCPMID: 22108384. Crystal structure of AcrB shows three AcrB protomers are organized as a homotrimer in the shape of a jellyfish. In the transmembrane region, each protomer has twelve transmembrane alpha-helicesPMID: 12374972.PMID: 11266632 PMID: 12374972 PMID: 15228545 PMID: 16079137 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRP (exp)YidCyesyesNo550.156TMH04.86972-1112="0"i12-32o340-359i366-390o396-416i437-458o470-493i541-557o875-892i899-919o925-946i975-995o1007-1029iCytoplasmicMembrane10
ACRE_ECOLIP24180
acrE

envC

envCb3265 JW3233 Acriflavine resistance protein E (Protein envC)Lipoprotein (Experimental)Proven pignal peptidase II substrate.PMID: 8472900 PMID: 15174130Inner Membrane LipoproteinEECell inner membrane | Lipid-anchorInner membrane lipoproteinN/ASECLOL: Localization Of LipoproteinsyesyesYes230.241SpII2310.9032Pos+2=N00Yn7-18c23/24oCytoplasmicMembrane9.9770.72
ACRF_ECOLIP24181
acrF

envD

envDb3266 JW3234 Acriflavine resistance protein F (Protein envD)Cell inner membrane (Probable) Integral inner membrane (Probable)PMID: 11274125 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo270.286SpI2612.2125-1211Yn10-21c26/27o340-359i366-390o396-416i437-458o470-497i541-558o872-891i898-918o924-948i969-990o1002-1028iCytoplasmicMembrane10
ACP_ECOLIP0A6A8
acpP
b1094 JW1080 Acyl carrier protein (ACP) (Cytosolic-activating factor) (CAF) (Fatty acid synthase acyl carrier protein)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of phospholipid metabolism complex PlsB-PssA-YbgC-AcpPMID: 16294310. Various methods were used to demonstrate that ACP could be cross-linked specifically to an inner membrane protein of E. coli, most probably to the glycerol-3-phosphate acyl transferase (GPAT)PMID: 1310877. Evidence for interactions of acyl carrier protein with glycerol-3-phosphate acyltransferase, an inner membrane protein of Escherichia coliPMID: 2676605. Membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier proteinPMID: 2668033. PMID: 3884600 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1310877 PMID: 2676605 PMID: 2668033 PMID: 3884600 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmic (Experimental)N/Apseudogene (Ochman & Davalos, 2006)noyesNo470.119CYT0-0.200913-00="0"iCytoplasmic10
ACPH_ECOLIP21515
acpH

yajB

yajBb0404 JW0394 Acyl carrier protein phosphodiesterase (ACP phosphodiesterase) (EC 3.1.4.14)CytoplasmicAACytoplasmicN/AnoyesNo240.265CYT0-0.200913-00Yn3-13c23/24oCytoplasmic8.9613.56
YBGC_ECOLIP0A8Z3
ybgC
b0736 JW0726 Acyl-CoA thioester hydrolase YbgC (Acyl-CoA thioesterase) (EC 3.1.2.-)Peripheral inner membrane protein facing the cytoplasm (Probable)Phospholipid metabolism complex PlsB-PssA-YbgC-acp PMID: 16294310Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicN/AyesyesNo520.116CYT0-0.200913-00="0"iCytoplasmic9.2670.38
YCIA_ECOLIP0A8Z0
yciA
b1253 JW1245 Acyl-CoA thioester hydrolase yciA (EC 3.1.2.-) (Protein P14)CytoplasmicAACytoplasmicN/AyesyesNo260.145CYT0-0.200913-00="0"iUnknown279.67
TESB_ECOLIP0AGG2
tesB
b0452 JW0442 Acyl-CoA thioesterase 2 (EC 3.1.2.-) (Acyl-CoA thioesterase II) (TEII)CytoplasmicAACytoplasmicN/AyesyesNo470.117CYT0-0.200913-00="0"iCytoplasmic9.2623.57
TESA_ECOLIP0ADA1
tesA

apeA pldC

apeA pldCb0494 JW0483 Acyl-CoA thioesterase I (EC 3.1.2.-) (Lecithinase B) (Lysophospholipase L1) (EC 3.1.1.5) (Protease I)Periplasmic (Experimental)PMID: 8098033PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesNo270.484SpI2611.0906-10Yn12-22c26/27oPeriplasmic1040.74
FADE_ECOLIQ47146
fadE

yafH

yafHb0221 JW5020 Acyl-coenzyme A dehydrogenase (ACDH) (EC 1.3.99.-)Trigger factor-sensitive aggregation.Integral Inner Membrane BBout/outIntegral Membrane ProteinIM (predicted)SRP,TFSECyesyesNo460.138TMH011.7565-22Yn3-13c17/18o27-58i279-296oCytoplasmicMembrane9.27
YCIY_ECOLIA5A613
yciY
b4595 Acyl-coenzyme A thioesterase paaI (EC 3.1.2.-) (Phenylacetic acid degradation protein paaI)CytoplasmicAACytoplasmicN/AnoyesNo180.1CYT0-0.200913-00="0"iUnknown2
LPXA_ECOLIP0A722
lpxA
b0181 JW0176 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (UDP-N-acetylglucosamine acyltransferase) (EC 2.3.1.129)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo250.206CYT0-0.200913-00="0"iCytoplasmic1021.62
ACYP_ECOLIP0AB65
yccX
b0968 JW5131 Acylphosphatase (EC 3.6.1.7) (Acylphosphate phosphohydrolase) Disulfide bridge (5 49) PMID: 17134700CytoplasmicAACytoplasmicN/AnoyesNo280.119CYT0-0.200913-00Yn4-11c16/17oCytoplasmic9.26103.06
ADEC_ECOLIP31441
ade

yicP

yicPb3665 JW3640 Adenine deaminase (Adenase) (Adenine aminase) (EC 3.5.4.2)CytoplasmicAACytoplasmicIM (predicted)noyesNo310.144CYT0-0.200913-00="0"iCytoplasmic8.9634.94
APT_ECOLIP69503
apt
b0469 JW0458 Adenine phosphoribosyltransferase (APRT) (EC 2.4.2.7)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo560.174CYT0-0.200913-00="0"iCytoplasmic9.9789.01
ADD_ECOLIP22333
add
b1623 JW1615 Adenosine deaminase (EC 3.5.4.4) (Adenosine aminohydrolase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo400.118CYT0-0.200913-00="0"iCytoplasmic9.9715.06
BIOA_ECOLIP12995
bioA
b0774 JW0757 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) (7,8-diamino-pelargonic acid aminotransferase) (DAPA AT) (DAPA aminotransferase) (Diaminopelargonic acid synthase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo550.168CYT0-0.200913-00="0"iCytoplasmic1021.69
CYAA_ECOLIP00936
cyaA

cya

cyab3806 JW3778 Adenylate cyclase (EC 4.6.1.1) (ATP pyrophosphate-lyase) (Adenylyl cyclase)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo260.196CYT0-0.200913-00="0"iCytoplasmic9.97
KAD_ECOLIP69441
adk

dnaW plsA

dnaW plsAb0474 JW0463 Adenylate kinase (AK) (EC 2.7.4.3) (ATP-AMP transphosphorylase)Cytoplasm (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)Adk cell localization has been monitored via fluorescence microscopy after protein was chromosomally tagged with fluorescent protein/s. These experiments indicate that Adk is distributed diffusely throughout cytoplasm. PMID: 8650159PMID: 17272300 PMID: 23230279 PMID: 8650159Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo180.12CYT0-0.200913-00Yn3-12c17/18oCytoplasmic10104.67
PUR8_ECOLIP0AB89
purB
b1131 JW1117 Adenylosuccinate lyase (ASL) (EC 4.3.2.2) (Adenylosuccinase) (ASase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo610.215CYT0-0.200913-00="0"iCytoplasmic9.9774.4
PURA_ECOLIP0A7D4
purA

adeK

adeKb4177 JW4135 Adenylosuccinate synthetase (AMPSase) (AdSS) (EC 6.3.4.4) (IMP--aspartate ligase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo620.102CYT0-0.200913-00="0"iCytoplasmic1041.37
CYSC_ECOLIP0A6J1
cysC
b2750 JW2720 Adenylyl-sulfate kinase (EC 2.7.1.25) (APS kinase) (ATP adenosine-5'-phosphosulfate 3'-phosphotransferase) (Adenosine-5'-phosphosulfate kinase)CytoplasmicAACytoplasmicIM (predicted)yesyesNo480.15CYT0-0.200913-00="0"iCytoplasmic9.9783.87
NUDE_ECOLIP45799
nudE

yrfE

yrfEb3397 JW3360 ADP compounds hydrolase nudE (EC 3.6.1.-)CytoplasmicAACytoplasmicN/AyesyesNo700.106CYT0-0.200913-00="0"iCytoplasmic9.9762.52
RFAF_ECOLIP37692
rfaF

waaF

waaFb3620 JW3595 ADP-heptose--LPS heptosyltransferase 2 (EC 2.-.-.-) (ADP-heptose--LPS heptosyltransferase II)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo420.134CYT0-0.200913-00="0"iCytoplasmic9.2624.65
HLDD_ECOLIP67910
hldD

htrM rfaD waaD

htrM rfaD waaDb3619 JW3594 ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20) (ADP-L-glycero-beta-D-manno-heptose-6-epimerase) (ADP-glyceromanno-heptose 6-epimerase) (ADP-hep 6-epimerase) (AGME)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)PMID: 23230279 PMID: 2198271Peripheral inner membrane protein facing the cytoplasmF1F1Cytoplasmic (Experimental)N/AyesyesNo190.195CYT0-0.200913-00="0"iCytoplasmic9.9726.48
ADPP_ECOLIQ93K97
nudF

aspP yqiE yzzG

aspP yqiE yzzGb3034 JW3002 ADP-ribose pyrophosphatase (EC 3.6.1.13) (ADP-ribose diphosphatase) (ADP-ribose phosphohydrolase) (ASPPase) (Adenosine diphosphoribose pyrophosphatase) (ADPR-PPase)Peripheral inner membrane protein facing the cytoplasm (Probable)Soluble protein, detected in inner membrane fractionPMID: 19766000.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble IM Fraction +yesyesNo380.101CYT0-0.200913-00="0"iCytoplasmic8.9657.62
DCTA_ECOLIP0A830
dctA
b3528 JW3496 Aerobic C4-dicarboxylate transport proteinIntegral inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPYidCnoyesNo190.136TMH05.74612-88="0"i9-27o47-64i76-99o141-164i184-208o220-242i326-344o350-373iCytoplasmicMembrane10
GLPD_ECOLIP13035
glpD

glyD

glyDb3426 JW3389 Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)Peripheral inner membrane protein facing the cytoplasm (Experimental) Inner membrane (Experimental) Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental)Binds to the cytoplasmic membrane through an amphipathic C-terminal alpha-helix (357-374)PMID: 11955283. Identified in PlsB-PflB-GlpD-Psd PMID: 20521846. Detergent-depleted enzyme can be functionally reconstituted with Escherichia coli membrane vesicles to support glycerol-3-phosphate-dependent active transport of L-prolinePMID: 340460. When GlpD is lost by mutation, the cells not only fail to grow on glycerol or l-alpha-glycerophosphate, but are subject to growth inhibition in the presence of either compound PMID: 5321485. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8982270 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 11955283 PMID: 340460 PMID: 15919657 PMID: 15911532 PMID: 8982270 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo350.138CYT0-0.200913-00="0"iCytoplasmic1048.25
ARCA_ECOLIP0A9Q1
arcA

cpxC dye fexA msp seg sfrA

cpxC dye fexA msp seg sfrAb4401 JW4364 Aerobic respiration control protein ArcA (Dye resistance protein)Peripheral inner membrane protein facing the cytoplasm (Probable) Nucleoid (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 18792681 PMID: 23138451 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmCytoplasmicN/AyesyesNo280.105CYT0-0.200913-00="0"iCytoplasmic1090.11
ARCB_ECOLIP0AEC3
arcB
b3210 JW5536 Aerobic respiration control sensor protein ArcB (EC 2.7.13.3)Cell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo360.18TMH09.51619-22="0"o20-46i58-77oCytoplasmicMembrane10
AER_ECOLIP50466
aer

air yqjJ

air yqjJb3072 JW3043 Aerotaxis receptorCell inner membrane (Probable) Integral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 22380631 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinMembrane anchoredIM (predicted), Detected in IM FractionSRPYidCyesyesNo340.205CYT0-0.200913-11="0"i171-200oCytoplasmicMembrane1031.17
SPEB_ECOLIP60651
speB
b2937 JW2904 Agmatinase (EC 3.5.3.11) (Agmatine ureohydrolase) (AUH)CytoplasmicAACytoplasmicN/AyesyesNo530.127CYT0-0.200913-00="0"iCytoplasmic9.9767.99
TQSA_ECOLIP0AFS5
tqsA

ydgG

ydgGb1601 JW1593 AI-2 transport protein tqsA (Transport of quorum-sensing signal protein)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo310.372TMH08.36419-88="0"i12-31o37-55i62-83o144-165i197-218o224-246i258-279o299-325iCytoplasmicMembrane10
ALR1_ECOLIP0A6B4
alr
b4053 JW4013 Alanine racemase, biosynthetic (EC 5.1.1.1)CytoplasmicAACytoplasmicN/AyesyesNo380.208CYT0-0.200913-00="0"iCytoplasmic9.9746.32
ALR2_ECOLIP29012
dadX

alnB dadB

alnB dadBb1190 JW1179 Alanine racemase, catabolic (EC 5.1.1.1)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo250.206CYT0-0.200913-00="0"iCytoplasmic102.69
SYA_ECOLIP00957
alaS

lovB

lovBb2697 JW2667 Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine--tRNA ligase) (AlaRS)Nucleoid (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23138451 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmCytoplasmicN/ATFnoyesNo260.129CYT0-0.200913-00="0"iCytoplasmic9.9757.55
YQHD_ECOLIQ46856
yqhD
b3011 JW2978 Alcohol dehydrogenase yqhD (EC 1.1.1.-)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo210.138CYT0-0.200913-00="0"iCytoplasmic9.9791.94
ADHP_ECOLIP39451
adhP

yddN

yddNb1478 JW1474 Alcohol dehydrogenase, propanol-preferring (EC 1.1.1.1)Peripheral Inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicIM (predicted-detected)yesyesNo400.124CYT0-0.200913-00="0"iCytoplasmic9.9786.56
ALDB_ECOLIP37685
aldB

yiaX

yiaXb3588 JW3561 Aldehyde dehydrogenase B (EC 1.2.1.-)CytoplasmicAACytoplasmicN/AnoyesNo380.103CYT0-0.200913-00="0"iCytoplasmic9.26
SAD_ECOLIP76149
sad

yneI

yneIb1525 JW5247 Aldehyde dehydrogenase-like protein yneI (EC 1.2.1.-)CytoplasmicAACytoplasmicN/AyesyesNo290.131CYT0-0.200913-00="0"iCytoplasmic9.9755.79
ADHE_ECOLIP0A9Q7
adhE

ana

anab1241 JW1228 Aldehyde-alcohol dehydrogenase [Includes: Alcohol dehydrogenase (ADH) (EC 1.1.1.1); Acetaldehyde dehydrogenase [acetylating] (ACDH) (EC 1.2.1.10); Pyruvate-formate-lyase deactivase (PFL deactivase)]Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo560.127CYT0-0.200913-00="0"iCytoplasmic9.2645.84
GAL1_ECOLIP0A6T3
galK

galA

galAb0757 JW0740 Aldose 1-epimerase (EC 5.1.3.3) (Galactose mutarotase) (Type-1 mutarotase)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo260.119CYT0-0.200913-00="0"iCytoplasmic8.9680.31
SSUB_ECOLIP0AAI1
ssuB

ycbE

ycbEb0933 JW0916 Aliphatic sulfonates import ATP-binding protein SsuB (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo220.181CYT0-0.200913-00="0"iCytoplasmicMembrane1023.53
PPB_ECOLIP00634
phoA
b0383 JW0374 Alkaline phosphatase (APase) (EC 3.1.3.1)Periplasm (Probable) Periplasmic (Experimental) (Experimental)Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544PMID: 3533724 PMID: 7022451 PMID: 3009404 PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECDsbAnoyesYes220.408SpI213.05238-00Yn5-16c21/22oPeriplasmic1078.02
IAP_ECOLIP10423
iap
b2753 JW2723 Alkaline phosphatase isozyme conversion protein (EC 3.4.11.-)Membrane associated (Experimental)PMID: 3316184Peripheral inner membrane protein facing the periplasmF2F2Membrane associated (Experimental)N/ASECyesyesYes250.707SpI2413.6553-00Yn9-20c24/25oUnknown4.925.51
SSUD_ECOLIP80645
ssuD

ycbN

ycbNb0935 JW0918 Alkanesulfonate monooxygenase (EC 1.14.14.5) (FMNH2-dependent aliphatic sulfonate monooxygenase) (Sulfate starvation-induced protein 6) (SSI6)CytoplasmicAACytoplasmicN/AyesyesNo390.14CYT0-0.200913-00="0"iCytoplasmic9.97
AHPC_ECOLIP0AE08
ahpC
b0605 JW0598 Alkyl hydroperoxide reductase subunit C (EC 1.11.1.15) (Alkyl hydroperoxide reductase protein C22) (Peroxiredoxin) (SCRP-23) (Sulfate starvation-induced protein 8) (SSI8) (Thioredoxin peroxidase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (47 47;166 166) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo490.111CYT0-0.200913-00="0"iCytoplasmic1090.35
AHPF_ECOLIP35340
ahpF
b0606 JW0599 Alkyl hydroperoxide reductase subunit F (EC 1.8.1.-) (Alkyl hydroperoxide reductase F52A protein)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (345 348) PMID: 19766000 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo350.108CYT0-0.200913-00="0"iCytoplasmicMembrane7.8876.12
ALLC_ECOLIP77425
allC

glxB7 ylbB

glxB7 ylbBb0516 JW0504 Allantoate amidohydrolase (EC 3.5.3.-) (Allantoate deiminase)CytoplasmicAACytoplasmicN/AnoyesNo240.136CYT0-0.200913-00="0"iCytoplasmic8.9625.43
ALLB_ECOLIP77671
allB

glxB3 ybbX

glxB3 ybbXb0512 JW0500 Allantoinase (EC 3.5.2.5) (Allantoin-utilizing enzyme)CytoplasmicAACytoplasmicN/AnoyesNo320.118CYT0-0.200913-00="0"iCytoplasmic9.97106.8
OTSA_ECOLIP31677
otsA
b1896 JW5312 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15) (Osmoregulatory trehalose synthesis protein A) (Trehalose-6-phosphate synthase) (UDP-glucose-glucosephosphate glucosyltransferase)Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo190.248CYT0-0.200913-00="0"iCytoplasmic1030.22
AMY1_ECOLIP25718
malS
b3571 JW3543 Alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase)Periplasmic (Experimental) Disulfide bridge (57 75;121 537) PMID: 9268356 Disulfide bond-containing protein PMID: 25664544PMID: 1544897PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes180.764SpI1714.5348-00Yn3-11c17/18oPeriplasmic1025.14
AGAL_ECOLIP06720
melA

mel-7

mel-7b4119 JW4080 Alpha-galactosidase (EC 3.2.1.22) (Melibiase)CytoplasmicAACytoplasmicN/AyesyesNo240.108CYT0-0.200913-00Yn7-18c23/24oCytoplasmic8.9620.86
YIHQ_ECOLIP32138
yihQ
b3878 JW3849 Alpha-glucosidase yihQ (EC 3.2.1.20)CytoplasmicAACytoplasmicN/AnoyesNo470.105CYT0-0.200913-00="0"iUnknown231.1
KGTP_ECOLIP0AEX3
kgtP

witA

witAb2587 JW2571 Alpha-ketoglutarate permeaseIntegral inner membrane (Probable)Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRP (exp)SECnoyesNo90.105TMH02.8422-1212="0"i21-42o62-84i96-116o122-139i160-183o195-214i244-266o278-299i311-331o337-360i372-393o405-423iCytoplasmicMembrane10
ALKB_ECOLIP05050
alkB

aidD

aidDb2212 JW2200 Alpha-ketoglutarate-dependent dioxygenase AlkB (EC 1.14.11.-) (Alkylated DNA repair protein AlkB)Nucleoid (Probable)DNA/RNA repair enzymes. AlkB repairs 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) lesions.PMID: 20084272NucleoidNNCytoplasmicIM (predicted)yesyesNo300.219CYT0-0.200913-00="0"iUnknown218.47
TAUD_ECOLIP37610
tauD

ssiD yaiG

ssiD yaiGb0368 JW0360 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17) (2-aminoethanesulfonate dioxygenase) (Sulfate starvation-induced protein 3) (SSI3)CytoplasmicAACytoplasmicN/AyesyesNo220.149CYT0-0.200913-00="0"iCytoplasmic9.9738.7
MNGB_ECOLIP54746
mngB

ybgG

ybgGb0732 JW0721 Alpha-mannosidase mngB (EC 3.2.1.-)CytoplasmicAACytoplasmicN/AnoyesNo210.101CYT0-0.200913-00="0"iUnknown24.39
COBC_ECOLIP52086
cobC

phpB

phpBb0638 JW0633 Alpha-ribazole phosphatase (EC 3.1.3.73) (Alpha-ribazole-5'-phosphate phosphatase)CytoplasmicAACytoplasmicN/AyesyesNo360.16CYT0-0.200913-00="0"iCytoplasmic9.9735.46
XYLS_ECOLIP31434
yicI
b3656 JW3631 Alpha-xylosidase (EC 3.2.1.-)CytoplasmicAACytoplasmicN/AnoyesNo250.174CYT0-0.200913-00="0"iUnknown231.7
UXAA_ECOLIP42604
uxaA

ygjW

ygjWb3091 JW3062 Altronate hydrolase (EC 4.2.1.7) (Altronic acid hydratase)Peripheral Inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicIM (predicted-detected)yesyesNo680.185CYT0-0.200913-00="0"iCytoplasmic8.96113.96
UXAB_ECOLIP0A6L7
uxaB
b1521 JW1514 Altronate oxidoreductase (EC 1.1.1.58) (Tagaturonate dehydrogenase) (Tagaturonate reductase)CytoplasmicAACytoplasmicN/AnoyesNo640.106CYT0-0.200913-00="0"iCytoplasmic9.9780.39
PUR1_ECOLIP0AG16
purF
b2312 JW2309 Amidophosphoribosyltransferase (ATase) (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (GPATase)Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo320.119CYT0-0.200913-00="0"iCytoplasmic9.9715.84
ARGA_ECOLIP0A6C5
argA
b2818 JW2786 Amino-acid acetyltransferase (EC 2.3.1.1) (N-acetylglutamate synthase) (AGS) (NAGS)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo600.101CYT0-0.200913-00="0"iCytoplasmic9.9757.9
PEPD_ECOLIP15288
pepD

pepH

pepHb0237 JW0227 Aminoacyl-histidine dipeptidase (EC 3.4.13.3) (Beta-alanyl-histidine dipeptidase) (Carnosinase) (Cysteinylglycinase) (Peptidase D) (Xaa-His dipeptidase) (X-His dipeptidase)CytoplasmicAACytoplasmicN/AyesyesNo290.15CYT0-0.200913-00="0"iCytoplasmic8.9687.7
ABGT_ECOLIP46133
abgT

ydaH

ydaHb1336 JW5822 ECK1332Aminobenzoyl-glutamate transport proteinCell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECnoyesNo550.352TMH03.72666-1313="0"i30-52o85-104i125-147o153-175i182-202o214-232i260-281o301-322i343-362o382-403i415-434o440-458i470-495oCytoplasmicMembrane10
ABGA_ECOLIP77357
abgA

ydaJ

ydaJb1338 JW5205 Aminobenzoyl-glutamate utilization protein ACytoplasmicAACytoplasmicN/AnoyesNo520.112CYT0-0.200913-00="0"iUnknown231.24
ABGB_ECOLIP76052
abgB

ydaI

ydaIb1337 JW1331 ECK1333Aminobenzoyl-glutamate utilization protein BCytoplasmicAACytoplasmicN/AnoyesNo610.103CYT0-0.200913-00="0"iUnknown249.36
PABC_ECOLIP28305
pabC
b1096 JW1082 Aminodeoxychorismate lyase (EC 4.1.3.38) (4-amino-4-deoxychorismate lyase) (ADC lyase) (ADCL)CytoplasmicAACytoplasmicN/AyesyesNo300.109CYT0-0.200913-00="0"iCytoplasmic9.2611.38
GCST_ECOLIP27248
gcvT
b2905 JW2873 Aminomethyltransferase (EC 2.1.2.10) (Glycine cleavage system T protein)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AnoyesNo310.116CYT0-0.200913-00="0"iCytoplasmic9.97101.03
AMPN_ECOLIP04825
pepN
b0932 JW0915 Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide hydrolase)Peripheral inner membrane protein facing the cytoplasm or cytoplasmic (By similarity) Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous peptidases are membrane associated in mammals, some data propose that the enzyme may associate with the E.coli membrane PMID: 323010. It is structurally classified with EC 3.4.11.2 membrane alanyl aminopeptidases. However, the extent of membrane association needs further analysis as it is generally soluble in the cytoplasmic fraction of E.coli and other bacteria. PMID: 323010 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 323010 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmic (Experimental)N/AyesyesNo500.108CYT0-0.200913-00="0"iCytoplasmicMembrane10101.76
YPDE_ECOLIP77585
ypdE
b2384 JW2381 Aminopeptidase ypdE (EC 3.4.11.-)CytoplasmicAACytoplasmicN/AyesyesNo240.106CYT0-0.200913-00="0"iCytoplasmic9.9792.69
YPDF_ECOLIP76524
ypdF
b2385 JW2382 Aminopeptidase ypdF (EC 3.4.11.-)CytoplasmicAACytoplasmicN/AyesyesNo190.122CYT0-0.200913-00="0"iCytoplasmic9.9732.88
YBDL_ECOLIP77806
ybdL
b0600 JW0593 Aminotransferase ybdL (EC 2.6.1.-)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo280.272SpI270.280409-00="0"iCytoplasmic9.268.95
AMTB_ECOLIP69681
amtB

ybaG

ybaGb0451 JW0441 Ammonia channel (Ammonia transporter) (Probable) Integral inner membrane (Experimental) Integral Membrane Protein (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 9720863 PMID: 10231487 PMID: 11847102 PMID: 14668330PMID: 11266632 PMID: 15361618 PMID: 11847102 PMID: 15919996 PMID: 9720863 PMID: 10231487 PMID: 14668330Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPYidCyesyesYes230.645SpI2212.8449-1211Yn3-14c22/23o32-54i66-86o120-142i149-171o183-205i217-236o248-268i280-297o303-324i336-356o376-396iCytoplasmicMembrane10
AMN_ECOLIP0AE12
amn
b1982 JW1963 AMP nucleosidase (EC 3.2.2.4)CytoplasmicAACytoplasmicN/AyesyesNo280.119CYT0-0.200913-00="0"iCytoplasmic9.9737.4
DCUA_ECOLIP0ABN5
dcuA

genA

genAb4138 JW5735 Anaerobic C4-dicarboxylate transporter dcuAIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPSECyesyesNo280.186TMH07.74612-1111="0"o6-38i45-66o86-109i130-152o164-190i225-243o255-277i289-306o326-345i357-381o410-431iCytoplasmicMembrane10
DCUB_ECOLIP0ABN9
dcuB

genF

genFb4123 JW4084 Anaerobic C4-dicarboxylate transporter dcuBIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPSECnoyesNo680.125TMH08.74519-1110Yn2-13c17/18o27-44i51-71o91-118i139-162o174-195i236-254o266-288i300-318o342-372i421-445oCytoplasmicMembrane10
DCUC_ECOLIP0ABP3
dcuC
b0621 JW0613/JW0616 Anaerobic C4-dicarboxylate transporter dcuCIntegral inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECnoyesNo560.117TMH00.537839-1212="0"i5-22o28-46i79-98o118-144i156-175o195-218i238-260o266-288i309-333o339-363i406-424o436-458iCytoplasmicMembrane103.79
DMSA_ECOLIP18775
dmsA
b0894 JW5118 Anaerobic dimethyl sulfoxide reductase chain A (DMSO reductase) (EC 1.8.99.-)Periplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm or the periplasm (Probable) Cell membrane, Peripheral membrane protein, Cytoplasmic side (Experimental) (Experimental)TAT signal peptide, dmsABC complex. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD. PMID: 24093457 Fluorescence analysis of these YFP chimeras revealed a wide range of interactions such as the one between the Tat substrate dimethyl sulfoxide reductase (DmsA) and its dedicated proofreading chaperone DmsD. PMID: 20169075 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395. TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332. PMID: 3280546 PMID: 3062312 Interacts with DmsD, a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 3062312 PMID: 11389150 PMID: 3280546 PMID: 20153451Peripheral inner membrane protein facing the periplasmF2F2Cell membrane | Peripheral membrane protein | Cytoplasmic sidePeriplasmic (Experimental)Soluble protein detected in Inner Membrane FractionDmsDTAT,SECyesyesNo460.537SpI455.24058-00="0"iCytoplasmicMembrane1022.52
DMSB_ECOLIP18776
dmsB
b0895 JW0878 Anaerobic dimethyl sulfoxide reductase chain B (DMSO reductase iron-sulfur subunit)Peripheral inner membrane protein facing the cytoplasm or the periplasm (Probable)TAT piggyback; DmsB is co-exported bound to Tat substrate DmsA. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332.PMID: 11389150Peripheral inner membrane protein facing the periplasmF2F2CytoplasmicN/ATAT piggybackyesyesNo440.107CYT0-0.200913-00="0"iUnknown5.4127.7
DMSC_ECOLIP18777
dmsC
b0896 JW0879 Anaerobic dimethyl sulfoxide reductase chain C (DMSO reductase anchor subunit)Integral inner membrane (Experimental) Integral inner membrane (Probable) Peripheral inner membrane protein facing the cytoplasm or the periplasm (Probable)DMSO reductase subunit C,Periplasmic. Has a membrane bound anchor. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 PMID: 15919996PMID: 15919996 PMID: 3062312 PMID: 11389150Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPSECyesyesNo220.177TMH013.558-88="0"o12-32i44-68o88-105i117-137o149-171i183-203o223-243i255-277oCytoplasmicMembrane10
YNFH_ECOLIP76173
ynfH
b1590 JW5261 Anaerobic dimethyl sulfoxide reductase chain ynfH (DMSO reductase anchor subunit ynfH)Integral inner membrane (Experimental)The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. ynfFGH are expressed anaerobically. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. YnfH can function with DmsAB, replacing DmsC as the anchor subunit. The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.PMID: 14522592.PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo340.15TMH013.2137-88="0"o12-34i46-67o87-104i116-138o150-169i181-204o224-243i250-271oCytoplasmicMembrane10
GLPA_ECOLIP0A9C0
glpA
b2241 JW2235 Anaerobic glycerol-3-phosphate dehydrogenase subunit A (G-3-P dehydrogenase) (EC 1.1.5.3)Peripheral inner membrane protein facing the cytoplasm (Probable) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo620.155CYT0-0.200913-00="0"iCytoplasmicMembrane105.6
GLPB_ECOLIP13033
glpB
b2242 JW2236 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (Anaerobic G-3-P dehydrogenase subunit B) (Anaerobic G3Pdhase B) (EC 1.1.5.3)Peripheral inner membrane protein facing the cytoplasm (Experimental) Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic (Experimental) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. PMID: 6387427 Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. PMID: 1987111 PMCID: PMC3210124 PMID: 3286606PMID: 20706981 PMID: 6387427 PMID: 1987111 PMCID: PMC3210124 PMID: 3286606Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)IM (predicted)yesyesNo300.137CYT0-0.200913-00Yn10-21c28/29oCytoplasmicMembrane1024.58
GLPC_ECOLIP0A996
glpC
b2243 JW2237 Anaerobic glycerol-3-phosphate dehydrogenase subunit C (G-3-P dehydrogenase)Peripheral inner membrane protein facing the cytoplasm (Probable) Membrane associated (Experimental) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. PMID: 3286606 PMID: 3286606Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinMembrane associated (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo350.222CYT0-0.200913-00="0"iCytoplasmicMembrane1013.54
NORV_ECOLIQ46877
norV

flrD ygaI ygaJ ygaK

flrD ygaI ygaJ ygaKb2710 JW2680 Anaerobic nitric oxide reductase flavorubredoxin (FlRd) (FlavoRb)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo180.103CYT0-0.200913-00="0"iCytoplasmic1039.49
NORR_ECOLIP37013
norR

ygaA

ygaAb2709 JW5843 Anaerobic nitric oxide reductase transcription regulator norRNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AyesyesNo230.113CYT0-0.200913-00="0"iCytoplasmic9.9730.34
NRDD_ECOLIP28903
nrdD
b4238 JW4197 Anaerobic ribonucleoside-triphosphate reductase (EC 1.17.4.2)CytoplasmicAACytoplasmicN/AyesyesNo590.103CYT0-0.200913-00="0"iCytoplasmic9.9759.55
NRDG_ECOLIP0A9N8
nrdG

yjgE

yjgEb4237 JW4196 Anaerobic ribonucleoside-triphosphate reductase-activating protein (EC 1.97.1.-) (Class III anaerobic ribonucleotide reductase small component)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo300.17CYT0-0.200913-00="0"iCytoplasmic1068.69
ASLB_ECOLIP25550
aslB

atsB

atsBb3800 JW5594 Anaerobic sulfatase-maturating enzyme homolog AslB (AnSME homolog)CytoplasmicAACytoplasmicN/AyesyesNo220.108CYT0-0.200913-00="0"iCytoplasmic9.9715.35
YDEM_ECOLIP76134
ydeM
b1497 JW1492 Anaerobic sulfatase-maturating enzyme homolog ydeM (AnSME homolog)CytoplasmicAACytoplasmicN/AyesyesNo320.118CYT0-0.200913-00="0"iCytoplasmic9.977.19
ANMK_ECOLIP77570
anmK

ydhH

ydhHb1640 JW1632 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.n1) (AnhMurNAc kinase)CytoplasmicAACytoplasmicN/AyesyesNo240.131CYT0-0.200913-00="0"iCytoplasmicMembrane9.8255.93
RCSB_ECOLIP0DMC7
rcsB
b2217 JW2205 Ankyrin repeat protein A (Ankyrin-like regulatory protein)Peripheral inner membrane protein facing the cytoplasm (Experimental) Nucleoid (Potential)Regulator protein RcsB is a member of the two-component regulatory system RcsC/RcsB. It is involved in the activation of colanic acid capsule synthesis (cps) and cell division (ftsZ) genes. It is believed that the RcsB protein is activated through phosphorylation, probably by the sensor protein RcsC. RcsB protein for Regulator capsule synthesis B, is a response regulator that belongs to the multicomponent RcsF/RcsC/RcsD/RcsA-RcsB phosphorelay system. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 2404948 Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AnoyesNo660.117CYT0-0.200913-00="0"iCytoplasmic1090.28
TRPE_ECOLIP00895
trpE
b1264 JW1256 Anthranilate synthase component 1 (EC 4.1.3.27) (Anthranilate synthase component I)Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo190.11CYT0-0.200913-00="0"iCytoplasmic1066.12
TRPG_ECOLIP00904
trpD

trpGD

trpGDb1263 JW1255 Anthranilate synthase component II (EC 4.1.3.27) [Includes: Glutamine amidotransferase; Anthranilate phosphoribosyltransferase (EC 2.4.2.18)]Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental)Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 4594441 PMID: 6283099PMID: 19766000 PMID: 15911532 PMID: 4594441 PMID: 6283099Peripheral inner membrane protein facing the cytoplasmF1F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo190.166CYT0-0.200913-00="0"iCytoplasmic9.2622.66
IRAD_ECOLIP39375
iraD

yjiD

yjiDb4326 JW5782 Anti-adapter protein iraDCytoplasmicAACytoplasmCytoplasmicN/AnoyesNo390.204CYT0-0.200913-00="0"iUnknown270.34
IRAM_ECOLIP75987
iraM

elb1 elbA ycgW

elb1 elbA ycgWb1160 JW1147 Anti-adapter protein iraM (Enhancing lycopene biosynthesis protein 1)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo230.126CYT0-0.200913-00="0"iUnknown254.91
IRAP_ECOLIP0AAN9
iraP

yaiB

yaiBb0382 JW0373 Anti-adapter protein iraPCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo420.126CYT0-0.200913-00="0"iUnknown283.98
AG43_ECOLIP39180
flu

yeeQ yzzX

yeeQ yzzXb2000 JW1982 Antigen 43 (AG43) (Fluffing protein) [Cleaved into: Antigen 43 alpha chain; Antigen 43 beta chain]Outer membrane protein (Experimental) (Probable) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (PMID: 15911532, PMID: 12624733, PMID: 10806384). Potential substrate of TAM secretion systemPMID: 22466966. Contains autotransporter beta-domain (IPR005546) PMID: 9298646PMID: 10806384 PMID: 15911532 PMID: 9298646Outer Membrane b-barrel proteinHHOuter Membrane β-barrel protein (Experimental)N/ASECAutotransporter(T5S), TAMProphage Integrase (EcoGene)nonoNo530.646SpI525.41594-10Yn34-45c52/53oOuterMembrane1032.89
REQ1_ECOLIQ47274
quuD

ybcQ

ybcQb0551 JW0539 Antitermination protein Q homolog from lambdoid prophage DLP12CytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo290.16CYT0-0.200913-00="0"iUnknown226.76
REQ2_ECOLIP76161
quuQ

ydfT

ydfTb1559 JW1551 Antitermination protein Q homolog from lambdoid prophage QinCytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo370.273CYT0-0.200913-00="0"iCytoplasmic8.9640.38
MQSA_ECOLIQ46864
mqsA

ygiT

ygiTb3021 JW2989 Antitoxin MqsANucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AnoyesNo670.113CYT0-0.200913-00="0"iCytoplasmic8.9646.04
RELB_ECOLIP0C079
relB
b1564 JW1556 Antitoxin RelBNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AProphage Integrase (EcoGene)nonoNo310.137CYT0-0.200913-00="0"iCytoplasmic1094.26
YJHH_ECOLIP39359
yjhH
b4298 JW5775 Antitoxin yefMCytoplasmicAACytoplasmicN/AnoyesNo520.11CYT0-0.200913-00="0"iPeriplasmic9.88.39
CITX_ECOLIP0A6G5
citX

ybdU

ybdUb0614 JW0606 Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.61) (Apo-ACP nucleodityltransferase) (Holo-ACP synthase) (Holo-citrate lyase synthase)CytoplasmicAACytoplasmicN/AyesyesNo480.133CYT0-0.200913-00="0"iCytoplasmic8.96
LNT_ECOLIP23930
lnt

cutE

cutEb0657 JW0654 Apolipoprotein N-acyltransferase (ALP N-acyltransferase) (EC 2.3.1.-) (Copper homeostasis protein cutE)Cell inner membrane (Probable)PMID: 2032623 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo280.184TMH013.3696-68="0"i12-28o34-50i57-79o91-112i124-142o162-188i195-214o489-507iCytoplasmicMembrane102.91
AQPZ_ECOLIP60844
aqpZ

bniP

bniPb0875 JW0859 Aquaporin Z (Bacterial nodulin-like intrinsic protein) (Probable) Integral inner membrane (Experimental) Cell inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidCPMID: 22108384. AqpZ forms tetramer in the inner membranePMID: 14691544. Sucrose gradient sedimentation analysis showed that the native, solubilized AqpZ protein is a trypsin-resistant tetramerPMID: 10518952. Upon reconstitution into proteoliposomes, AqpZ displays very high osmotic water permeability. No permeation by glycerol, urea or sorbitol was detectedPMID: 10518952. Electron crystallography has shown monomers to consist of six membrane spanning alpha-helices confirming sequence based predictionsPMID: 10487750.PMID: 11266632 PMID: 14691544 PMID: 10518952 PMID: 10487750 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo430.268TMH014.8231-65Yn5-17c26/27o36-60i81-103o129-149i161-180o206-226iCytoplasmicMembrane10
KDSD_ECOLIP45395
kdsD

yrbH

yrbHb3197 JW3164 Arabinose 5-phosphate isomerase (EC 5.3.1.13)CytoplasmicAACytoplasmicIM (predicted)yesyesNo690.13CYT0-0.200913-00="0"iCytoplasmic8.9662.19
ARAG_ECOLIP0AAF3
araG
b1900 JW1888 Arabinose import ATP-binding protein AraG (EC 3.6.3.17)Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 7028715Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo380.293CYT0-0.200913-00="0"iCytoplasmicMembrane1037.9
ARAC_ECOLIP0A9E0
araC
b0064 JW0063 Arabinose operon regulatory proteinNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasm.CytoplasmicN/AnoyesNo290.152CYT0-0.200913-00="0"iCytoplasmic1027.75
ARAE_ECOLIP0AE24
araE
b2841 JW2809 Arabinose-proton symporter (Arabinose transporter)Integral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo450.136TMH07.37397-1212="0"i23-51o63-84i91-110o116-137i149-167o179-198i258-281o297-317i329-348o360-384i405-422o428-446iCytoplasmicMembrane10
ARTM_ECOLIP0AE30
artM
b0861 JW0845 Arginine ABC transporter permease protein ArtMIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo270.106TMH012.0162-55="0"o20-39i51-70o82-100i155-176o188-211iCytoplasmicMembrane10
ARTQ_ECOLIP0AE34
artQ
b0862 JW0846 Arginine ABC transporter permease protein ArtQIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo300.173TMH017.1037-45="0"o12-35i42-68o101-118i161-182o202-223iCytoplasmicMembrane1026.25
ARGO_ECOLIP11667
argO

yggA

yggAb2923 JW2890 Arginine exporter protein ArgOIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein(experimental)IM (predicted)SRPSECyesyesNo250.273TMH06.65864-66="0"o6-26i38-61o67-88i109-127o147-170i182-199oCytoplasmicMembrane1018.85
ASTA_ECOLIP0AE37
astA

ydjV

ydjVb1747 JW1736 Arginine N-succinyltransferase (AST) (EC 2.3.1.109) (AOST)CytoplasmicAACytoplasmicN/AyesyesNo340.16CYT0-0.200913-00="0"iCytoplasmic9.9712.07
ARGR_ECOLIP0A6D0
argR

xerA

xerAb3237 JW3206 Arginine repressorNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasm.CytoplasmicN/AyesyesNo620.115CYT0-0.200913-00="0"iCytoplasmic1090.79
ARTP_ECOLIP0AAF6
artP
b0864 JW0848 Arginine transport ATP-binding protein ArtP (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) The artPIQM artJ genes encode a third arginine-uptake system in addition to the known argT hisJQMP system of Salmonella typhimurium and E. coli and the arginine (-ornithine) carrier (aps) of E. coli. The artQ, artM and artP products are similar to the transmembraneous proteins and the ATPase of binding-protein-dependent carriers. PMID: 8801422 PMID: 8801422 PMID: 15758229 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 8801422 PMID: 15758229 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo200.124CYT0-0.200913-00="0"iCytoplasmicMembrane7.8826.62
ADIC_ECOLIP60061
adiC

aniC yjdD yjdE

aniC yjdD yjdEb4115 JW4076 Arginine/agmatine antiporterIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo620.127TMH08.65108-1212="0"i12-35o41-61i91-116o122-141i153-173o193-217i229-254o274-296i317-340o352-374i386-404o410-428iCytoplasmicMembrane10
ARLY_ECOLIP11447
argH
b3960 JW3932 Argininosuccinate lyase (ASAL) (EC 4.3.2.1) (Arginosuccinase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo530.138CYT0-0.200913-00="0"iCytoplasmic9.97
ASSY_ECOLIP0A6E4
argG
b3172 JW3140 Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo370.163CYT0-0.200913-00="0"iCytoplasmic9.9719.39
SYR_ECOLIP11875
argS
b1876 JW1865 Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA ligase) (ArgRS)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo230.136CYT0-0.200913-00="0"iCytoplasmic9.9724.19
AROP_ECOLIP15993
aroP
b0112 JW0108 Aromatic amino acid transport protein AroP (General aromatic amino acid permease)Integral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo440.121TMH09.5384-1212="0"i20-41o47-63i84-105o125-142i154-176o196-220i241-263o283-300i332-353o359-379i400-422o428-446iCytoplasmicMembrane10
TYRB_ECOLIP04693
tyrB
b4054 JW4014 Aromatic-amino-acid aminotransferase (ARAT) (AROAT) (EC 2.6.1.57) Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo100.122CYT0-0.200913-00="0"iCytoplasmic1038.03
ARSC_ECOLIP0AB96
arsC

arsG

arsGb3503 JW3470 Arsenate reductase (EC 1.20.4.1) (Arsenical pump modifier)CytoplasmicAACytoplasmicN/AyesyesNo140.101CYT0-0.200913-00="0"iCytoplasmic9.9792.41
ARSB_ECOLIP0AB93
arsB

arsF

arsFb3502 JW3469 Arsenical pump membrane protein (Arsenic efflux pump protein)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECnoyesNo440.23TMH08.42262-1110Yn2-17c35/36o51-70i91-109o115-132i139-163o175-198i222-240o246-263i275-296o316-334i401-423oCytoplasmicMembrane10
ARSR_ECOLIP37309
arsR

arsE

arsEb3501 JW3468 Arsenical resistance operon repressorNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasm.CytoplasmicN/Apseudogene (Ochman & Davalos, 2006)nonoNo400.112CYT0-0.200913-00Yn3-10c14/15oUnknown228.86
ASLA_ECOLIP25549
aslA

atsA

atsAb3801 JW3773 Arylsulfatase (AS) (EC 3.1.6.1) (Aryl-sulfate sulphohydrolase)PeriplasmicGGSecretedPeriplasmicN/ASECnoyesYes250.743SpI2417.5099-00Yn10-20c24/25oUnknown6.4925.41
ULAA_ECOLIP39301
ulaA

sgaT yjfS

sgaT yjfSb4193 JW5744 Ascorbate-specific permease IIC component ulaA (Ascorbate-specific PTS system EIIC component)Cell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo200.105TMH03.53199-1010="0"o15-34i46-66o100-121i142-170o233-253i265-285o305-331i338-359o379-402i423-445oCytoplasmicMembrane10
ULAC_ECOLIP69820
ulaC

ptxA sgaA yjfU

ptxA sgaA yjfUb4195 JW4153 Ascorbate-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (PTS system ascorbate-specific EIIA component)Peripheral inner membrane protein facing the cytoplasm (Potential)Peripheral inner membrane protein facing the cytoplasm.PMID: 12644495Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AnoyesNo470.126CYT0-0.200913-00="0"iCytoplasmicMembrane7.8884.35
ULAB_ECOLIP69822
ulaB

sgaB yjfT

sgaB yjfTb4194 JW4152 Ascorbate-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (Ascorbate-specific PTS system EIIB component)Peripheral inner membrane protein facing the cytoplasm (By similarity)The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectivelyPMID: 12644495. All three proteins are required for the anaerobic utilization and uptake of L-ascorbate in vivo and its phosphoenolpyruvate-dependent phosphorylation in vitroPMID: 12644495.PMID: 12644495Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AnoyesNo620.14CYT0-0.200913-00="0"iUnknown253.44
ASNB_ECOLIP22106
asnB
b0674 JW0660 Asparagine synthetase B [glutamine-hydrolyzing] (EC 6.3.5.4)Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo370.141CYT0-0.200913-00="0"iCytoplasmic9.97
SYN_ECOLIP0A8M0
asnS

tss

tssb0930 JW0913 Asparaginyl-tRNA synthetase (EC 6.1.1.22) (Asparagine--tRNA ligase) (AsnRS)Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/ATFyesyesNo530.182CYT0-0.200913-00="0"iCytoplasmic1070.21
PAND_ECOLIP0A790
panD
b0131 JW0127 Aspartate 1-decarboxylase (EC 4.1.1.11) (Aspartate alpha-decarboxylase) [Cleaved into: Aspartate 1-decarboxylase beta chain; Aspartate 1-decarboxylase alpha chain]Cytoplasm (Probable)PMID: 6767707CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo620.203CYT0-0.200913-00="0"iUnknown275.2
AAT_ECOLIP00509
aspC
b0928 JW0911 Aspartate aminotransferase (AspAT) (EC 2.6.1.1) (Transaminase A)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)PMID: 23230279 PMID: 3521591Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmic (Experimental)N/AyesyesNo230.126CYT0-0.200913-00="0"iCytoplasmic10
ASPA_ECOLIP0AC38
aspA
b4139 JW4099 Aspartate ammonia-lyase (Aspartase) (EC 4.3.1.1)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected with chromosomally tap-tagged TF with >80% confidence score PMID: 15690043PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo220.123CYT0-0.200913-00="0"iCytoplasmic9.9787.37
PYRB_ECOLIP0A786
pyrB
b4245 JW4204 Aspartate carbamoyltransferase catalytic chain (EC 2.1.3.2) (Aspartate transcarbamylase) (ATCase)Trigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo340.111CYT0-0.200913-00="0"iCytoplasmic9.9778.75
PYRI_ECOLIP0A7F3
pyrI
b4244 JW4203 Aspartate carbamoyltransferase regulatory chainCytoplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 3912513 PMID: 8740179CytoplasmicAACytoplasmic (Experimental)N/AyesyesNo370.104CYT0-0.200913-00="0"iUnknown2116.96
ASNA_ECOLIP00963
asnA
b3744 JW3722 Aspartate--ammonia ligase (EC 6.3.1.1) (Asparagine synthetase A)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo450.101CYT0-0.200913-00="0"iCytoplasmic1090.31
DHAS_ECOLIP0A9Q9
asd

hom

homb3433 JW3396 Aspartate-semialdehyde dehydrogenase (ASA dehydrogenase) (ASADH) (EC 1.2.1.11)Peripheral Inner membrane protein facing the cytoplasm (Potential) Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicIM (predicted-detected)yesyesNo440.197CYT0-0.200913-00="0"iCytoplasmic9.2618.65
SYD_ECOLIP21889
aspS

tls

tlsb1866 JW1855 Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase) (AspRS)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)SecY-interacting protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19139097 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo270.104CYT0-0.200913-00="0"iCytoplasmic1081.96
HIS1_ECOLIP60757
hisG
b2019 JW2001 ATP phosphoribosyltransferase (ATP-PRT) (ATP-PRTase) (EC 2.4.2.17)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo440.181CYT0-0.200913-00="0"iCytoplasmic1064.44
ATPE_ECOLIP0A6E6
atpC

papG uncC

papG uncCb3731 JW3709 ATP synthase epsilon chain (ATP synthase F1 sector epsilon subunit) (F-ATPase epsilon subunit)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 21778229 PMID: 9868784 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionnoyesNo410.107CYT0-0.200913-00="0"iCytoplasmicMembrane10120.16
ATPG_ECOLIP0ABA6
atpG

papC uncG

papC uncGb3733 JW3711 ATP synthase gamma chain (ATP synthase F1 sector gamma subunit) (F-ATPase gamma subunit)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 7508444 PMID: 2900236 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 7508444 PMID: 2900236 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo410.162CYT0-0.200913-00="0"iCytoplasmicMembrane1048.82
ATPZ_ECOLIP0ABC0
atpI

uncI

uncIb3739 JW5611 ATP synthase protein IIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo640.113TMH04.01303-43Yn14-25c30/31o40-59i71-94o100-122iCytoplasmicMembrane1034.91
ATP6_ECOLIP0AB98
atpB

papD uncB

papD uncBb3738 JW3716 ATP synthase subunit a (ATP synthase F0 sector subunit a) (F-ATPase subunit 6)Integral inner membrane (Probable) Integral Membrane Protein (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 10806384PMID: 15919996 PMID: 10806384Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPYidCyesyesNo670.123CYT0-0.200913-55="0"o36-60i100-118o147-166i211-233o239-264iCytoplasmicMembrane107.26
ATPA_ECOLIP0ABB0
atpA

papA uncA

papA uncAb3734 JW3712 ATP synthase subunit alpha (EC 3.6.3.14) (ATP synthase F1 sector subunit alpha) (F-ATPase subunit alpha)Peripheral inner membrane protein facing the cytoplasm (Probable) Inner membrane (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicIM (predicted)TFYidCnoyesNo280.106CYT0-0.200913-00="0"iCytoplasmic1060.67
ATPF_ECOLIP0ABA0
atpF

papF uncF

papF uncFb3736 JW3714 ATP synthase subunit b (ATP synthase F(0) sector subunit b) (ATPase subunit I) (F-type ATPase subunit b) (F-ATPase subunit b)Membrane anchored (Experimental)PMID: 6278247Integral Inner Membrane BBout/inCell inner membrane | Single-pass membrane proteinMembrane anchored (Experimental)Soluble protein detected in Inner Membrane FractionSRPSECnoyesNo320.35CYT0-0.200913-11="0"o6-26iCytoplasmicMembrane1019.55
ATPB_ECOLIP0ABB4
atpD

papB uncD

papB uncDb3732 JW3710 ATP synthase subunit beta (EC 3.6.3.14) (ATP synthase F1 sector subunit beta) (F-ATPase subunit beta)Inner membrane (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionYidCyesyesNo230.123CYT0-0.200913-00="0"iCytoplasmicMembrane1077.87
ATPL_ECOLIP68699
atpE

papH uncE

papH uncEb3737 JW3715 ATP synthase subunit c (ATP synthase F(0) sector subunit c) (Dicyclohexylcarbodiimide-binding protein) (F-type ATPase subunit c) (F-ATPase subunit c) (Lipid-binding protein)Integral inner membrane (Probable) Integral Membrane Protein (Experimental)Experimentally verified gene-productPMID: 9868784. Methionine start codon is cleavedPMID: 9868784. Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreasedPMID: 15263011PMID: 21778229. PMID: 9237612PMID: 15919996 PMID: 9237612Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPYidCnoyesNo280.117TMH07.95599-22="0"o12-32i53-76oCytoplasmicMembrane1056.33
ATPD_ECOLIP0ABA4
atpH

papE uncH

papE uncHb3735 JW3713 ATP synthase subunit delta (ATP synthase F(1) sector subunit delta) (F-type ATPase subunit delta) (F-ATPase subunit delta)Peripheral inner membrane protein facing the cytoplasm (Probable) Inner membrane (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo360.135CYT0-0.200913-00="0"iCytoplasmic1089.37
PHNN_ECOLIP16690
phnN
b4094 JW4055 ATP-binding protein phnNCytoplasmicAACytoplasmicN/AnoyesNo450.118CYT0-0.200913-00="0"iCytoplasmic9.264.48
CYDC_ECOLIP23886
cydC

mdrA mdrH surB

mdrA mdrH surBb0886 JW0869 ATP-binding/permease protein cydCIntegral inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo500.199TMH016.2776-66="0"i16-35o41-61i133-155o161-182i243-266o278-301iCytoplasmicMembrane1010.22
CYDD_ECOLIP29018
cydD

htrD

htrDb0887 JW0870 ATP-binding/permease protein cydDInner membrane (Experimental) Integral inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919657 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPYidCyesyesNo430.111TMH00.800558-56="0"i29-49o61-82i140-159o165-188i245-272o278-297iCytoplasmicMembrane10
CLPS_ECOLIP0A8Q6
clpS

yljA

yljAb0881 JW0865 ATP-dependent Clp protease adapter protein ClpSCytoplasmicAACytoplasmicN/AyesyesNo530.102CYT0-0.200913-00="0"iCytoplasmic1062.56
CLPA_ECOLIP0ABH9
clpA

lopD

lopDb0882 JW0866 ATP-dependent Clp protease ATP-binding subunit ClpACytoplasmicAACytoplasmicN/AnoyesNo180.153CYT0-0.200913-00="0"iCytoplasmic1060.56
CLPX_ECOLIP0A6H1
clpX

lopC

lopCb0438 JW0428 ATP-dependent Clp protease ATP-binding subunit ClpXCytoplasmicAACytoplasmicN/AyesyesNo310.124CYT0-0.200913-00="0"iCytoplasmic9.9769.98
CLPP_ECOLIP0A6G7
clpP

lopP

lopPb0437 JW0427 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) (Caseinolytic protease) (Endopeptidase Clp) (Heat shock protein F21.5) (Protease Ti)CytoplasmicAACytoplasmCytoplasmicIM (predicted)noyesNo310.108CYT0-0.200913-00="0"iCytoplasmic10
RECG_ECOLIP24230
recG

radC

radCb3652 JW3627 ATP-dependent DNA helicase recG (EC 3.6.4.12)Cytoplasm (Experimental) Nucleoid (Experimental) YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300 Has a role in constitutive stable DNA replication (CsdR) and R-loop formation. 7774596PMID: 17272300 7774596NucleoidNNCytoplasmicN/AyesyesNo200.141CYT0-0.200913-00="0"iCytoplasmic9.97
RECQ_ECOLIP15043
recQ
b3822 JW5855 ATP-dependent DNA helicase recQ (EC 3.6.4.12)CytoplasmicAACytoplasmicN/AnoyesNo50.114CYT0-0.200913-00="0"iCytoplasmic9.9747.29
REP_ECOLIP09980
rep
b3778 JW5604 ATP-dependent DNA helicase rep (EC 3.6.4.12)NucleoidNNCytoplasmicN/AyesyesNo230.124CYT0-0.200913-00="0"iCytoplasmic9.9719.24
HSLU_ECOLIP0A6H5
hslU

htpI

htpIb3931 JW3902 ATP-dependent protease ATPase subunit HslU (Heat shock protein HslU) (Unfoldase HslU)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo240.141CYT0-0.200913-00="0"iCytoplasmic1074.44
HSLV_ECOLIP0A7B8
hslV

htpO yiiC

htpO yiiCb3932 JW3903 ATP-dependent protease subunit HslV (EC 3.4.25.2) (Heat shock protein HslV)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo300.118CYT0-0.200913-00="0"iCytoplasmic10
HRPA_ECOLIP43329
hrpA
b1413 JW5905 ATP-dependent RNA helicase hrpA (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo520.107CYT0-0.200913-00="0"iCytoplasmic8.96
HRPB_ECOLIP37024
hrpB

yadO

yadOb0148 JW0144 ATP-dependent RNA helicase hrpB (EC 3.6.4.13)CytoplasmicAACytoplasmicN/AyesyesNo320.17CYT0-0.200913-00Yn6-17c26/27oCytoplasmicMembrane9.823.75
RHLB_ECOLIP0A8J8
rhlB

mmrA

mmrAb3780 JW3753 ATP-dependent RNA helicase rhlB (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental) Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 23230279 PMID: 18337249 PMID: 24438330Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AyesyesNo560.132CYT0-0.200913-00="0"iCytoplasmic9.97
RHLE_ECOLIP25888
rhlE
b0797 JW0781 ATP-dependent RNA helicase rhlE (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental)Assembly cofactor of ribosome; associates with the 70S subunit. Overexpression of protein suppresses defects in deletion of DeaD. PMID: 18083833PMID: 23230279 PMID: 18083833Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo460.14CYT0-0.200913-00="0"iCytoplasmic9.9762.62
SRMB_ECOLIP21507
srmB

rbaB

rbaBb2576 JW2560 ATP-dependent RNA helicase srmB (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (Experimental) Inner membrane (Experimental)Assembly cofactor of ribosome; associate with the 50S subunit.PMID: 23230279 PMID: 15919657Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo330.109CYT0-0.200913-00="0"iCytoplasmic1068.57
FTSH_ECOLIP0AAI3
ftsH

hflB mrsC std tolZ

hflB mrsC std tolZb3178 JW3145 ATP-dependent zinc metalloprotease FtsH (EC 3.4.24.-) (Cell division protease FtsH)Cell inner membrane (Probable) Inner membrane (Experimental) Integral inner membrane (Probable) Integral Membrane Protein (Experimental)Trigger factor-sensitive aggregation. PMID: 8444797 PMID: 1925026 PMID: 8106505 PMID: 8106505 PMID: 7781608PMID: 8444797 PMID: 8947034 PMID: 15919657 PMID: 15919996 PMID: 1925026 PMID: 8106505 PMID: 7781608Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRP,TFSECnoyesNo240.152SpI212.2847-21Yn5-16c21/22o98-119iCytoplasmicMembrane1041.14
DBPA_ECOLIP21693
dbpA
b1343 JW1337 ATP-independent RNA helicase dbpA (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; Fragment of 23S rRNA. Assembly cofactor of ribosome; Fragment of 23S rRNA. PMID: 21529161PMID: 21529161Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo320.341CYT0-0.200913-00="0"iCytoplasmic9.9712.57
RAVA_ECOLIP31473
ravA

yieN

yieNb3746 JW3725 ATPase ravA (EC 3.6.3.-) (Regulatory ATPase variant A)CytoplasmicAACytoplasmCytoplasmicIM (predicted)noyesNo690.182CYT0-0.200913-00="0"iCytoplasmic1027.09
DGAL_ECOLIP0AEE5
mglB
b2150 JW2137 Autoinducer 2 import ATP-binding protein lsrA (AI-2 import ATP-binding protein lsrA) (EC 3.6.3.-) (EGO10A)Periplasmic (Experimental) Potential Skp substrate PMID: 19953543 PMID: 7012152 PMID: 9298646PMID: 7012152 PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECSkpnoyesYes240.861SpI2323.7963-10Yn5-16c23/24oPeriplasmic1075.23
HOKB_ECOLIP77494
hokB

ydcB

ydcBb4428 JW5225 Autoinducer 2 import system permease protein lsrC (AI-2 import system permease protein lsrC)Potentially toxic protein.Integral Inner Membrane BBout/inMembrane | Single-pass membrane proteinMembrane anchoredN/ASRPSECnoyesNo260.105CYT0-0.200913-11="0"o6-24iPeriplasmic9.9642.15
IBSD_ECOLIC1P616
ibsD
b4664 JW3023.1 Autoinducer 2 import system permease protein lsrD (AI-2 import system permease protein lsrD)Belongs to the toxic protein ibs family. Potentially toxic protein.Integral Inner Membrane BB-Membrane | Single-pass membrane protein-N/ASECnoyesNo180.098CYT0-0.200913-10Yn4-9c13/14oCytoplasmicMembrane9.82
LSRK_ECOLIP77432
lsrK

ydeV

ydeVb1511 JW1504 Autoinducer 2 kinase lsrK (AI-2 kinase) (EC 2.7.1.-)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo270.149CYT0-0.200913-00="0"iCytoplasmic9.266.02
LSRB_ECOLIP76142
lsrB

yneA

yneAb1516 JW1509 Autoinducer 2-binding protein lsrB (AI-2-binding protein lsrB)Periplasmic (Experimental)PMID: 15601708PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes270.845SpI2621.9126-00Yn9-20c26/27oPeriplasmic1075.63
LSRG_ECOLIP64461
lsrG

yneC

yneCb1518 JW1511 Autoinducer 2-degrading protein lsrG (AI-2-degrading protein lsrG)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo330.101CYT0-0.200913-00="0"iUnknown276.67
GRCA_ECOLIP68066
grcA

yfiD

yfiDb2579 JW2563 Autonomous glycyl radical cofactorPeripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo300.139CYT0-0.200913-00="0"iCytoplasmic9.97
BFR_ECOLIP0ABD3
bfr
b3336 JW3298 Bacterioferritin (BFR) (Cytochrome b-1) (Cytochrome b-557)CytoplasmicAACytoplasmicN/AnoyesNo300.153CYT0-0.200913-00="0"iCytoplasmic1095.71
BFD_ECOLIP0AE56
bfd

yheA

yheAb3337 JW3299 Bacterioferritin-associated ferredoxinCytoplasmicAACytoplasmicN/AnoyesNo180.109CYT0-0.200913-00="0"iCytoplasmic10
OMPC_ECOLIP06996
ompC

meoA par

meoA parb2215 JW2203 Bacteriophage N4 adsorption protein AOuter membrane protein (Experimental) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein related to resistance to ampicillin and tetracycline; Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 15911532 PMID: 10806384 PMID: 9298646Outer Membrane b-barrel proteinHHCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)Soluble protein detected in Inner Membrane FractionTFSECSkpBAM: β-Barrel Assembly MachineryyesyesYes220.884SpI2121.1188-00Yn5-16c21/22oOuterMembrane106.56
YBHT_ECOLIP0AAW9
ybhT
b0762 JW5102 Bacteriophage N4 adsorption protein BSmall membrane protein (Probable) Outer membrane (Probable)Experimentally verified orientation: N(out)-C(in). Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased.PMID: 19121005 PMID: 21778229Integral Inner Membrane BBin/outMembrane | Single-pass membrane proteinMembrane anchoredIM (predicted)SRPYidCnoyesNo210.157TMH01.49031-11="0"i7-29oCytoplasmicMembrane9.8228.74
NFRB_ECOLIP0AFA5
nfrB
b0569 JW0558 Bacteriophage N4 adsorption protein BCell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo200.134CYT0-0.200913-44="0"o12-42i365-384o390-410i422-443oCytoplasmicMembrane107.03
LIT_ECOLIP11072
lit
b1139 JW1125 Bacteriophage T4 late gene expression-blocking protein (GpLit)Single weak hydrophobic region. Could be a transmembrane domain but could also be a peripheral inner membrane protein.Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AProphage Integrase (EcoGene)nonoNo200.181CYT0-0.200913-01="0"i61-81oCytoplasmic8.96
GTRB_ECOLIP77293
yfdH
b2351 JW2347 Bactoprenol glucosyl transferase homolog from prophage CPS-53 (EC 2.4.1.-)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECProphage Integrase (EcoGene), pseudogene (Ochman & Davalos, 2006)nonoNo470.115CYT0-0.200913-22="0"i228-249o261-286iCytoplasmicMembrane10
GTRA_ECOLIP77682
yfdG
b2350 JW2346 Bactoprenol-linked glucose translocase homolog from prophage CPS-53Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECProphage Integrase (EcoGene)nonoNo380.341TMH011.761-44="0"i12-30o36-55i67-85o91-110iCytoplasmicMembrane10
FLGD_ECOLIP75936
flgD

fla FIV flaV

fla FIV flaVb1075 JW1062 Basal-body rod modification protein flgDCell surface appendage (Experimental)Hook-capping protein. The hook cap FlgD is displaced by FlgK, allowing filament growth PMID: 12631703 Using the hook scaffolding protein FlgD of Escherichia coli as a model substrate, it is demonstrated that the export signal is contained within the N-terminal 71 amino acids of FlgD. PMID: 16897036 PMID: 16897036PMID: 12631703Peripheral outer membrane protein facing the extra-cellular spaceF4F4SecretedCell surface appendage (Experimental)N/AFlagellum Flapseudogene (Ochman & Davalos, 2006)yesnoNo290.171SpI282.5996-00="0"iExtracellular10106.45
BEPA_ECOLIP66948
yfgC
b2494 JW2479 Beta-barrel assembly-enhancing proteasePeriplasm (Potential)BepA maintains the integrity of OM both by promoting assembly of OMPs and by proteolytically eliminating OMPs when their correct assembly was compromised. BepA is a metalloprotease that cleaves its targets preferentially between Phe-Phe residues. Potential Skp substrate PMID: 19953543PMID: 22491786PeriplasmicGGPeriplasmicN/ASECSkpyesyesYes280.636SpI2715.2429-00Yn9-19c27/28oUnknown2.510.5
BGAL_ECOLIP00722
lacZ
b0344 JW0335 Beta-galactosidase (Beta-gal) (EC 3.2.1.23) (Lactase)CytoplasmicAACytoplasmicN/AyesyesNo360.136CYT0-0.200913-00="0"iUnknown241.96
BGLR_ECOLIP05804
uidA

gurA gusA

gurA gusAb1617 JW1609 Beta-glucuronidase (GUS) (EC 3.2.1.31) (Beta-D-glucuronoside glucuronosohydrolase)Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo590.12CYT0-0.200913-00="0"iCytoplasmic8.9646.03
NAGZ_ECOLIP75949
nagZ

ycfO

ycfOb1107 JW1093 Beta-hexosaminidase (EC 3.2.1.52) (Beta-N-acetylhexosaminidase) (N-acetyl-beta-glucosaminidase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo400.114CYT0-0.200913-00="0"iCytoplasmic1011.52
YDBC_ECOLIP25906
ydbC
b1406 JW1403 Beta-ketoadipyl-CoA thiolase (EC 2.3.1.174) (3-oxoadipyl-CoA thiolase)CytoplasmicAACytoplasmicN/AyesyesNo380.123CYT0-0.200913-00="0"iCytoplasmic9.2661.28
BLR_ECOLIP56976
blr
b4409 JW5963 Beta-lactam resistance proteinCell inner membrane (Probable)Small membrane protein detected experimentally.PMID: 10931331 PMID: 22885295Integral Inner Membrane BBin/outCell inner membrane | Single-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo270.106CYT0-0.200913-11="0"i7-26oCytoplasmicMembrane10
AMPC_ECOLIP00811
ampC

ampA

ampAb4150 JW4111 Beta-lactamase (EC 3.5.2.6) (Cephalosporinase)Periplasmic (Experimental)PMID: 6795623PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes200.854SpI1917.1519-00Yn4-15c19/20oPeriplasmic1026.22
BETB_ECOLIP17445
betB
b0312 JW0304 Betaine aldehyde dehydrogenase (BADH) (EC 1.2.1.8)CytoplasmicAACytoplasmicN/AnoyesNo410.128CYT0-0.200913-00="0"iCytoplasmic9.9733.6
BCR_ECOLIP28246
bcr

bicA bicR sur suxA

bicA bicR sur suxAb2182 JW5363 Bicyclomycin resistance protein (Sulfonamide resistance protein)Integral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo330.128TMH013.9575-1212="0"i7-26o46-65i77-96o102-123i135-159o165-185i215-241o253-273i285-307o313-332i344-366o372-391iCytoplasmicMembrane10
SPOT_ECOLIP0AG24
spoT
b3650 JW3625 Bifunctional (p)ppGpp synthase/hydrolase SpoT [Includes: GTP pyrophosphokinase (EC 2.7.6.5) ((p)ppGpp synthase) (ATP:GTP 3'-pyrophosphotransferase) (Stringent response-like protein) (ppGpp synthase II); Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydroCytoplasm (Probable)PMID: 17616600CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo690.124CYT0-0.200913-00="0"iCytoplasmic8.968.32
COBU_ECOLIP0AE76
cobU
b1993 JW1971 Bifunctional adenosylcobalamin biosynthesis protein CobU (Adenosylcobinamide kinase) (EC 2.7.1.156) (Adenosylcobinamide-phosphate guanylyltransferase) (EC 2.7.7.62)CytoplasmicAACytoplasmicN/AnoyesNo200.144CYT0-0.200913-00="0"iCytoplasmic9.9762.13
AK1H_ECOLIP00561
thrA

thrA1 thrA2

thrA1 thrA2b0002 JW0001 Bifunctional aspartokinase/homoserine dehydrogenase 1 (Aspartokinase I/homoserine dehydrogenase I) (AKI-HDI) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.3)]Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental)Copurifies with trigger factor in pull down assays. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 4562989 PMID: 354697 PMID: 7003595 PMID: 6777249PMID: 15911532 PMID: 4562989 PMID: 354697 PMID: 7003595 PMID: 6777249Peripheral inner membrane protein facing the cytoplasmF1F1Cytoplasmic (Experimental)N/ATFyesyesNo150.142CYT0-0.200913-00="0"iCytoplasmic8.96
AK2H_ECOLIP00562
metL

metM

metMb3940 JW3911 Bifunctional aspartokinase/homoserine dehydrogenase 2 (Aspartokinase II/homoserine dehydrogenase II) (AKII-HDII) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.3)]Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo240.117CYT0-0.200913-00="0"iCytoplasmicMembrane9.82
GSP_ECOLIP0AES0
gsp
b2988 JW2956 Bifunctional glutathionylspermidine synthetase/amidase [Includes: Glutathionylspermidine synthase (EC 6.3.1.8) (GSP synthetase) (Glutathione:spermidine ligase [ADP-forming]); Glutathionylspermidine amidase (EC 3.5.1.78) (Glutathionylspermidine amidohydrolCytoplasmicAAMembrane anchoredN/AyesyesNo250.115CYT0-0.200913-10="0"iCytoplasmic8.9650.4
ARNA_ECOLIP77398
arnA

pmrI yfbG

pmrI yfbGb2255 JW2249 Bifunctional polymyxin resistance protein ArnA (Polymyxin resistance protein PmrI) [Includes: UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13) (ArnAFT) (UDP-L-Ara4N formyltransferase); UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acidPeripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AnoyesNo590.178CYT0-0.200913-00="0"iCytoplasmic9.973.48
AAS_ECOLIP31119
aas
b2836 JW2804 Bifunctional protein aas [Includes: 2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) (2-acyl-GPE acyltransferase) (Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase); Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20) (Acyl-ACP sIntegral membrane protein (experimental; from GenProtEC) Exhibites aberrant localization by microscopy in the absence of YidC.Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinSoluble protein detected in Inner Membrane FractionSRPYidCyesyesNo570.217CYT0-0.200913-02="0"o258-277i415-438oCytoplasmicMembrane107.49
BIRA_ECOLIP06709
birA

bioR dhbB

bioR dhbBb3973 JW3941 Bifunctional protein BirA [Includes: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase (EC 6.3.4.15) (Biotin--protein ligase)]. Deletion of BioR resulted in a 15-fold increase of bio operon transcription in the presence of high biotin levels.Nucleoid (Experimental)PMID: 23138451Nucleoid, Integral Inner Membrane N, BN,BCytoplasmicIM (predicted)SRPSECyesyesNo260.122CYT0-0.200913-02="0"i123-139o145-162iCytoplasmic1037.71
FOLC_ECOLIP08192
folC

dedC

dedCb2315 JW2312 Bifunctional protein folC [Includes: Folylpolyglutamate synthase (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) (Tetrahydrofolate synthase); Dihydrofolate synthase (EC 6.3.2.12)] Disulfide bridge (354 401) CytoplasmicAACytoplasmicN/AyesyesNo260.113CYT0-0.200913-00="0"iCytoplasmic9.9784.67
FOLD_ECOLIP24186
folD

ads

adsb0529 JW0518 Bifunctional protein folD [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)]CytoplasmicAACytoplasmicN/AyesyesNo490.145CYT0-0.200913-00="0"iCytoplasmic9.9744.45
GLMU_ECOLIP0ACC7
glmU

yieA

yieAb3730 JW3708 Bifunctional protein glmU [Includes: UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) (N-acetylglucosamine-1-phosphate uridyltransferase); Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)]Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo490.123CYT0-0.200913-00="0"iCytoplasmic1094.89
HLDE_ECOLIP76658
hldE

rfaE waaE yqiF

rfaE waaE yqiFb3052 JW3024 Bifunctional protein hldE [Includes: D-beta-D-heptose 7-phosphate kinase (EC 2.7.1.-) (D-beta-D-heptose 7-phosphotransferase); D-beta-D-heptose 1-phosphate adenosyltransferase (EC 2.7.7.-)]CytoplasmicAACytoplasmicN/AyesyesNo680.175CYT0-0.200913-00="0"iCytoplasmic9.9777.16
PUTA_ECOLIP09546
putA

poaA

poaAb1014 JW0999 Bifunctional protein putA [Includes: Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase); Delta-1-pyrroline-5-carboxylate dehydrogenase (P5C dehydrogenase) (EC 1.5.1.12)]Peripheral inner membrane protein facing the cytoplasm (By similarity) Nucleoid protein (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)The proline dehydrogenase PutA protein from S. typhimurium was shown to act as a membrane-associated enzyme or as a transcriptional repressor PMID: 8483946. Catabolism of proline requires PutA to associate with the membrane because reoxidation of the FAD cofactor in PutA needs the presence of an electron acceptor. Repression of the put operon requires PutA to bind to the put control-region DNA in the cytoplasm. Transcription factor PMID: 23138451. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 8483946 PMID: 23138451 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo640.118CYT0-0.200913-00="0"iCytoplasmic9.97
PUR9_ECOLIP15639
purH
b4006 JW3970 Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (ATIC) (IMP synthase) (Inosinicase)]CytoplasmicAACytoplasmicN/AyesyesNo270.151CYT0-0.200913-00="0"iCytoplasmic9.9772.06
ADIA_ECOLIP28629
adiA

adi

adib4117 JW5731 Biodegradative arginine decarboxylase (ADC) (EC 4.1.1.19)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo210.114CYT0-0.200913-00="0"iCytoplasmic1030.94
PGAD_ECOLIP69432
pgaD

ycdP

ycdPb1021 JW1006 Biofilm PGA synthesis protein PgaDIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo380.135TMH010.5144-22="0"i16-36o56-74iCytoplasmicMembrane109.97
BSSR_ECOLIP0AAY1
bssR

yliH

yliHb0836 JW0820 Biofilm regulator BssRCytoplasmicAACytoplasmicN/AyesyesNo230.138CYT0-0.200913-00="0"iUnknown251.51
BSSS_ECOLIP0AB33
bssS

yceP

ycePb1060 JW5152 Biofilm regulator BssSCytoplasmicAACytoplasmicN/AyesyesNo390.109CYT0-0.200913-00="0"iUnknown288.62
EXBB_ECOLIP0ABU7
exbB
b3006 JW2974 Biopolymer transport protein exbBIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo400.153CYT0-0.200913-33="0"o21-43i132-157o177-198iCytoplasmicMembrane1014.97
EXBD_ECOLIP0ABV2
exbD
b3005 JW2973 Biopolymer transport protein exbDIntegral Inner Membrane BBout/inCell inner membrane | Single-pass type II membrane proteinMembrane anchoredIM (predicted), Detected in IM FractionSRPSECyesyesNo420.15TMH00.162291-11="0"o20-41iCytoplasmicMembrane9.9747.5
SPEA_ECOLIP21170
speA
b2938 JW2905 Biosynthetic arginine decarboxylase (ADC) (EC 4.1.1.19)Peripheral inner membrane protein facing the cytoplasm (Experimental) Periplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Probable) Periplasm (Experimental)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 2198270 Selective disruption of the cell envelope and an assessment of Spea activity or immunoprecipitable SpeA in various fractions demonstrated its location between the cytoplasmic membrane and peptidoglycan layer. PMID: 3894328PMID: 15919657 PMID: 2198270 PMID: 19766000 PMID: 3894328PeriplasmicGGSecretedCytoplasmicSoluble protein detected in Inner Membrane FractionTFSEC, non classicalyesyesNo270.129CYT0-0.200913-00="0"iPeriplasmic1048
BCCP_ECOLIP0ABD8
accB

fabE

fabEb3255 JW3223 Biotin carboxyl carrier protein of acetyl-CoA carboxylase (BCCP)CytoplasmicAACytoplasmicN/AyesyesNo500.616CYT0-0.200913-00="0"iCytoplasmic8.9695.13
ACCC_ECOLIP24182
accC

fabG

fabGb3256 JW3224 Biotin carboxylase (EC 6.3.4.14) (Acetyl-CoA carboxylase subunit A) (ACC) (EC 6.4.1.2)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation PMID: 19737520.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo360.117CYT0-0.200913-00="0"iCytoplasmic9.9729.79
BISC_ECOLIP20099
bisC
b3551 JW5940 Biotin sulfoxide reductase (EC 1.-.-.-) (BDS reductase) (BSO reductase)CytoplasmicAACytoplasmicN/AyesyesNo240.128CYT0-0.200913-00="0"iPeriplasmic9.44
BIOB_ECOLIP12996
bioB
b0775 JW0758 Biotin synthase (EC 2.8.1.6)CytoplasmicAACytoplasmicIM (predicted)yesyesNo330.13CYT0-0.200913-00="0"iCytoplasmic9.2658.7
BIOC_ECOLIP12999
bioC
b0777 JW0760 Biotin synthesis protein BioCCytoplasmicAACytoplasmicN/AyesyesNo240.14CYT0-0.200913-00="0"iUnknown26.32
APAH_ECOLIP05637
apaH
b0049 JW0048 Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] (EC 3.6.1.41) (Ap4A hydrolase) (Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase) (Diadenosine tetraphosphatase)CytoplasmicAACytoplasmicN/AyesyesNo290.105CYT0-0.200913-00="0"iCytoplasmic9.2627.11
CUEO_ECOLIP36649
cueO

yacK

yacKb0123 JW0119 Blue copper oxidase cueO (Copper efflux oxidase)Periplasmic (Experimental)TAT secretion system. PMID: 9298646PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ATAT,SECyesyesYes290.821SpI2816.1248-00Yn11-22c28/29oPeriplasmic106.35
YCGF_ECOLIP75990
ycgF
b1163 JW1150 Blue light- and temperature-regulated antirepressor YcgF (Blrp)CytoplasmicAACytoplasmicN/AnoyesNo490.111CYT0-0.200913-00="0"iCytoplasmicMembrane7.8820.39
BRNQ_ECOLIP0AD99
brnQ
b0401 JW0391 Branched-chain amino acid transport system 2 carrier protein (LIV-II)Integral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo250.304TMH015.0757-1212="0"i12-29o41-64i76-98o118-138i150-171o191-212i224-246o281-307i314-333o339-359i371-389o409-426iCytoplasmicMembrane10
ILVE_ECOLIP0AB80
ilvE
b3770 JW5606 Branched-chain-amino-acid aminotransferase (BCAT) (EC 2.6.1.42) (Transaminase B)CytoplasmicAACytoplasmicN/AyesyesNo390.117CYT0-0.200913-00="0"iCytoplasmic9.9730.57
CHAA_ECOLIP31801
chaA
b1216 JW1207 Calcium/proton antiporterIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECyesyesNo620.229TMH01.59472-1111="0"i15-32o38-60i72-94o106-131i143-164o170-188i221-240o252-269i290-313o319-340i347-365oCytoplasmicMembrane10
ARPA_ECOLIP23325
arpA

arp yjaC

arp yjaCb4017 JW3977 Capsular synthesis regulator component BCytoplasmicAACytoplasmicN/AnoyesNo210.126CYT0-0.200913-00="0"iUnknown2
ARCC_ECOLIP37306
arcC

ybcF

ybcFb0521 JW0510 Carbamate kinase (EC 2.7.2.2)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo220.244CYT0-0.200913-00Yn3-14c19/20oCytoplasmic9.2616.07
ARCM_ECOLIP77624
yahI
b0323 JW0315 Carbamate kinase-like protein yahIPeripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo200.12CYT0-0.200913-00="0"iCytoplasmic9.9782.53
ARCL_ECOLIQ46807
yqeA
b2874 JW2842 Carbamate kinase-like protein yqeACytoplasmicAACytoplasmicN/AyesyesNo200.148SpI152.26791-00Yn5-10c15/16oCytoplasmic9.9797.34
CARB_ECOLIP00968
carB

pyrA

pyrAb0033 JW0031 Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase ammonia chain)Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo240.228CYT0-0.200913-00Yn9-16c21/22oUnknown4.99
CARA_ECOLIP0A6F1
carA

pyrA

pyrAb0032 JW0030 Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase glutamine chain)Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo220.124CYT0-0.200913-00="0"iCytoplasmic9.9760.46
HYPF_ECOLIP30131
hypF

hydA

hydAb2712 JW5433 Carbamoyltransferase hypF (EC 2.1.3.-) (Carbamoyl phosphate-converting enzyme hypF) ([NiFe]-hydrogenase maturation factor hypF) (Hydrogenase maturation protein hypF) Carbamoyltransferase, HypF-type (IPR004421) CytoplasmicAACytoplasmicN/AnoyesNo200.113CYT0-0.200913-00="0"iCytoplasmic8.9673.66
CDAR_ECOLIP37047
cdaR

sdaR yaeG

sdaR yaeGb0162 JW5013 Carbohydrate diacid regulator (Sugar diacid regulator)Nucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AyesyesNo310.103CYT0-0.200913-00="0"iCytoplasmic9.978.59
FUCA_ECOLIP0AB87
fucA

fucC prd

fucC prdb2800 JW2771 Carbon starvation protein ACytoplasmicAACytoplasmicN/AnoyesNo280.115CYT0-0.200913-00="0"iCytoplasmic9.9754.19
CSRA_ECOLIP69913
csrA

zfiA

zfiAb2696 JW2666 Carbon storage regulatorCytoplasmicAACytoplasmicN/AnoyesNo370.101CYT0-0.200913-00="0"iUnknown2
CYNT_ECOLIP0ABE9
cynT
b0339 JW0330 Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonate dehydratase 1)CytoplasmicAACytoplasmicN/AnoyesNo460.101CYT0-0.200913-00="0"iCytoplasmic9.2646.89
CAN_ECOLIP61517
can

cynT2 yadF

cynT2 yadFb0126 JW0122 Carbonic anhydrase 2 (EC 4.2.1.1) (Carbonate dehydratase 2)CytoplasmicAACytoplasmicN/AyesyesNo330.123CYT0-0.200913-00="0"iCytoplasmic9.9747.63
CAAL_ECOLIP77213
ybdK
b0581 JW0570 Carboxylate-amine ligase ybdK (EC 6.3.-.-)CytoplasmicAACytoplasmicN/AyesyesNo320.11CYT0-0.200913-00="0"iCytoplasmic9.978.96
BIOH_ECOLIP13001
bioH

bioB

bioBb3412 JW3375 Carboxylesterase BioH (EC 3.1.1.1) (Biotin synthesis protein BioH)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo270.241CYT0-0.200913-00="0"iCytoplasmic9.9764.28
CLSA_ECOLIP0A6H8
clsA

cls nov yciJ

cls nov yciJb1249 JW1241 Cardiolipin synthase (CL synthase) (EC 2.7.8.-)Cell inner membrane (Probable) (Experimental) In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. PMID: 22988102PMID: 15919996 PMID: 22988102Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo220.149TMH05.41966-22="0"o6-25i37-58oCytoplasmicMembrane103.69
CAIE_ECOLIP39206
caiE
b0035 JW5004 Carnitine operon protein CaiECytoplasmicAACytoplasmicN/AyesyesNo270.148CYT0-0.200913-00="0"iCytoplasmic9.2618.67
CAID_ECOLIP31551
caiD

yaaL

yaaLb0036 JW0035 Carnitinyl-CoA dehydratase (EC 4.2.1.-) (Crotonobetainyl-CoA hydratase)CytoplasmicAACytoplasmicN/AyesyesNo260.176CYT0-0.200913-00="0"iCytoplasmic9.9715.36
CRP_ECOLIP0ACJ8
crp

cap csm

cap csmb3357 JW5702 Catabolite gene activator (cAMP receptor protein) (cAMP regulatory protein)Peripheral inner membrane protein facing the cytoplasm (Experimental) Nucleoid protein (Probable)Trigger factor-sensitive aggregation.PMID: 15650111 PMID: 23138451 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/ATFyesyesNo240.105CYT0-0.200913-00="0"iCytoplasmic9.97
CATE_ECOLIP21179
katE
b1732 JW1721 Catalase HPII (EC 1.11.1.6) (Hydroxyperoxidase II)Trigger factor-sensitive aggregation.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo480.103CYT0-0.200913-00="0"iCytoplasmic1043.37
KATG_ECOLIP13029
katG
b3942 JW3914 Catalase-peroxidase (CP) (EC 1.11.1.6) (EC 1.11.1.7) (Hydroperoxidase I) (HPI) (Peroxidase/catalase)Trigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo300.121CYT0-0.200913-00="0"iCytoplasmic1084.35
FIU_ECOLIP75780
fiu

ybiL

ybiLb0805 JW0790 Catecholate siderophore receptor fiu (Ferric iron uptake protein) (TonB-dependent receptor fiu)Outer Membrane β-barrel protein (Experimental) Outer membrane protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544PMID: 10806384 PMID: 20932056Outer Membrane b-barrel proteinHHSingle-pass type I membrane proteinOuter Membrane β-barrel protein (Experimental)N/ASECSkpBAM: β-Barrel Assembly MachineryyesyesYes340.525SpI3311.8291-10Yn14-25c33/34oOuterMembrane1010.6
CUSA_ECOLIP38054
cusA

ybdE

ybdEb0575 JW0564 Cation efflux system protein cusAIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo390.202TMH06.2187-1212="0"i12-32o338-357i364-384o390-411i442-464o484-505i538-554o874-891i898-918o924-948i985-1006o1012-1037iCytoplasmicMembrane10
CUSB_ECOLIP77239
cusB

ylcD

ylcDb0574 JW0563 Cation efflux system protein cusBMFP family function as auxiliary proteins or 'adaptors', connecting a primary porter in the cytoplasmic membrane of a Gram-negative bacterium with an outer membrane factor (OMF; TC #1.B.17) protein that serves a porin or channel function in the outer membrane.Peripheral inner membrane protein facing the periplasmF2F2Membrane anchoredN/ASECyesyesNo290.243SpI287.5302-10Yn4-16c28/29oUnknown2.556.44
CUSC_ECOLIP77211
cusC

ibeB ylcB

ibeB ylcBb0572 JW0561 Cation efflux system protein cusCLipoprotein (Experimental) Cell outer membrane (Probable) Outer mebrane protein (Experimental) Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130 PMID: 21249122 PMID: 20932056Outer Membrane LipoproteinIICell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)N/ASECLOL: Localization Of LipoproteinsyesyesYes220.405SpII1711.8848Pos+2=S00Yn6-15c20/21oOuterMembrane1027.76
CUSF_ECOLIP77214
cusF

cusX ylcC

cusX ylcCb0573 JW0562 Cation efflux system protein cusFPeriplasmic (Experimental)PMID: 12813074PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes230.444SpI228.68332-00Yn4-15c22/23oPeriplasmic10
CHAC_ECOLIP39163
chaC
b1218 JW1209 Cation transport protein ChaCCytoplasmicAACytoplasmicN/AyesyesNo320.137CYT0-0.200913-00="0"iUnknown227.74
CHAB_ECOLIP0AE63
chaB
b1217 JW1208 Cation transport regulator ChaBCytoplasmicAACytoplasmicN/AnoyesNo220.289CYT0-0.200913-00="0"iUnknown2111.56
ACTP_ECOLIP32705
actP

yjcG

yjcGb4067 JW4028 Cation/acetate symporter ActP (Acetate permease) (Acetate transporter ActP)Cell inner membrane (Probable) Integral inner membrane (Experimental)PMID: 16079137 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesYes190.597SpI1816.2988-1313Yn4-14c18/19o34-54i75-97o103-122i148-167o179-197i209-227o262-286i298-323o355-383i404-423o429-453i465-486o498-517iCytoplasmicMembrane10
CDH_ECOLIP06282
cdh
b3918 JW3889 CDP-diacylglycerol pyrophosphatase (EC 3.6.1.26) (CDP-diacylglycerol phosphatidylhydrolase) (CDP-diglyceride hydrolase)PeriplasmicGGCell inner membrane | Single-pass membrane proteinMembrane anchoredSoluble protein detected in Inner Membrane FractionSECyesyesNo190.264SpI197.43557-10Yn4-14c19/20oCytoplasmicMembrane1055.48
PGSA_ECOLIP0ABF8
pgsA
b1912 JW1897 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5) (Phosphatidylglycerophosphate synthase) (PGP synthase)Integral inner membrane (Experimental)Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 9008159 PMID: 12558186 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRP (exp)SECyesyesNo350.17TMH012.5336-46="0"i7-27o33-52i64-83o89-108i120-142o148-169iCytoplasmicMembrane107.29
PSS_ECOLIP23830
pssA

pss

pssb2585 JW2569 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) (Phosphatidylserine synthase)Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid metabolism complex PlsB-PssA-YbgC-Acp. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicN/ATFyesyesNo480.108CYT0-0.200913-00="0"iCytoplasmic1035.8
CEDA_ECOLIP0AE60
cedA

ydjP

ydjPb1731 JW1720 Cell division activator CedACytoplasmicAACytoplasmicN/AyesyesNo610.153CYT0-0.200913-00="0"iUnknown2107.67
FTSE_ECOLIP0A9R7
ftsE
b3463 JW3428 Cell division ATP-binding protein ftsEPeripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Is the putative ATP-binding protein component of an ATP-binding cassette (ABC) transporter. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. PMID: 10048040 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 10048040 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo320.118CYT0-0.200913-00="0"iCytoplasmicMembrane1011.64
SULA_ECOLIP0AFZ5
sulA

sfiA

sfiAb0958 JW0941 Cell division inhibitor sulASeptal ring (Probable) SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ.PMID: 12808143CytoplasmicAACytoplasmicN/AyesyesNo400.133CYT0-0.200913-00="0"iCytoplasmicMembrane1061.09
FTSA_ECOLIP0ABH0
ftsA

divA

divAb0094 JW0092 Cell division protein ftsAPeripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)FtsA has a conserved C-terminal membrane targeting sequence (MTS) with extremely weak TmHMM and Phobius predictions. Deletion or point mutations in the MTS, inactivate FtsA function and inhibit cell division. Because FtsA binds to the tubulin-like FtsZ protein that forms the Z ring, it is thought that the MTS of FtsA is required, along with the transmembrane protein ZipA, to assemble the Z ring and anchor it to the cytoplasmic membrane. FtsA function in cell division depends on additive effects of membrane binding and self-interaction, and that the specific requirement of an amphipathic helix for tethering FtsA to the membrane can be bypassed. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18186792 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo250.12CYT0-0.200913-00="0"iCytoplasmic9.9784.72
FTSB_ECOLIP0A6S5
ftsB

ygbQ

ygbQb2748 JW2718 Cell division protein ftsBCell inner membrane (Probable)PMID: 11972052 PMID: 15165235Integral Inner Membrane BBout/inCell inner membrane | Single-pass type II membrane proteinMembrane anchoredIM (predicted)SRPSECyesyesNo230.157CYT0-0.200913-10Yn4-14c18/19oCytoplasmicMembrane9.8225.62
FTSL_ECOLIP0AEN4
ftsL

mraR yabD

mraR yabDb0083 JW0081 Cell division protein ftsLLipoprotein (Experimental) Integral Inner Membrane (Probable) Cell inner membrane (Probable)Localizes to potential division sites in filamentous cells. Proven pignal peptidase II substrate. PMID: 1332942PMID: 15174130 PMID: 21890895 PMID: 1332942 PMID: 10027987 PMID: 11703663 PMID: 11948172 PMID: 15165235Inner Membrane LipoproteinEECell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)IM (predicted)SEC, non classicalyesyesNo240.167CYT0-0.200913-11="0"o35-57iCytoplasmic8.9625.32
FTSN_ECOLIP29131
ftsN

msgA

msgAb3933 JW3904 Cell division protein ftsNIntegral inner membrane (Probable)Localizes to potential division sites in filamentous cells. FtsN interacts directly with itself, FtsW, FtsI and ZapA.PMID: 11703663 PMID: 20497333Integral Inner Membrane BBin/outCell inner membrane | Single-pass type II membrane proteinMembrane anchoredSoluble protein detected in Inner Membrane FractionSRPSECyesyesNo410.126SpI400.332384-11="0"i29-54oCytoplasmicMembrane1023.63
FTSQ_ECOLIP06136
ftsQ
b0093 JW0091 Cell division protein ftsQCell inner membrane (Probable) (Experimental)FtsQ could be localized to the division site in constricting cells. FtsQ insertion requires SecYEG, the transmembrane electrical potential, SecA, and the SRP pathway. PMID: 15546656 YidC plays a role in the membrane integration of the FtsQ protein as the hydrophobic region of FtsQ can be cross-linked to YidC. PMID: 15546656PMID: 2007547 PMID: 9829918 PMID: 9882666 PMID: 11415986 PMID: 11703663 PMID: 11948172 PMID: 15165235 PMID: 17693520 PMID: 15546656Integral Inner Membrane BBin/outCell inner membrane | Single-pass type II membrane proteinMembrane anchoredIM (predicted)SRPSECyesyesNo410.115TMH01.18618-11="0"i25-49oUnknown224.26
FTSW_ECOLIP0ABG4
ftsW
b0089 JW0087 Cell division protein ftsWInner membrane (Probable) Cell inner membrane (Probable) Integral inner membrane (Experimental)Interacts directly with FtsN. The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex.PMID: 20847002 PMID: 9603865 PMID: 12423747 PMID: 11807049 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo670.137TMH010.7628-109Yn15-26c32/33o48-66i87-104o110-129i175-192o198-215i222-239o307-328i340-364o376-395iCytoplasmicMembrane10
FTSX_ECOLIP0AC30
ftsX

ftsS

ftsSb3462 JW3427 Cell division protein ftsXCell inner membrane (Probable) Integral inner membrane (Experimental)Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 3323846 PMID: 10048040 PMID: 14729705 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRP (exp)SECyesyesNo390.105CYT0-0.200913-44="0"o72-95i214-240o272-290i323-342oCytoplasmicMembrane1023.24
FTSY_ECOLIP10121
ftsY
b3464 JW3429 Cell division protein ftsYPeripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains an essential and autonomous membrane-binding amphipathic helix. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18281057 PMID: 8194520 PMID: 9177162 PMID: 11353766 PMID: 17726013 PMID: 19414018 PMID: 21543314 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo30.107CYT0-0.200913-00="0"iCytoplasmicMembrane10
FTSZ_ECOLIP0A9A6
ftsZ

sfiB sulB

sfiB sulBb0095 JW0093 Cell division protein ftsZPeripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental) (Experimental)Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. Assembles at midcell at the inner surface of the cytoplasmic membrane. SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. PMID: 8917533 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Over-expression of NlpI can interrupt the nucleoids division and the assembly of FtsZ at the septum, mediated by IbpA/IbpB, suggesting a role of the NlpI/IbpA/IbpB complex in the cell division. PMID: 25699035PMID: 1944597 PMID: 20497333 PMID: 8917533 PMID: 23230279 PMID: 25699035Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo260.12CYT0-0.200913-00="0"iCytoplasmicMembrane1079.85
ZAPA_ECOLIP0ADS2
zapA

ygfE

ygfEb2910 JW2878 Cell division protein zapA (Z ring-associated protein zapA)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental)Binds to the FtsZ ring. ZapA interacts directly with itself, FtsN, FtsZ and FtsI. Peripheral inner membrane protein via binding to the FtsZ ring. ZapA orthologue, YgfE, binds FtsZ polymers and promotes FtsZ polymer bundling. PMID: 12368265PMID: 20487275 PMID: 20497333 PMID: 17428494 PMID: 12368265Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo670.152CYT0-0.200913-00="0"iUnknown297.8
ZAPB_ECOLIP0AF36
zapB

yiiU

yiiUb3928 JW3899 Cell division protein zapBPeripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental)PMID: 20487275 PMID: 18394147Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AnoyesNo190.122CYT0-0.200913-00="0"iUnknown281.78
ZAPD_ECOLIP36680
zapD

yacF

yacFb0102 JW0099 Cell division protein ZapDCytoplasm (Probable) Peripherally acossiated with the inner membrane, cytoplasmic side (Experimental) Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity. 22505682PMID: 22505682 22505682Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo390.116CYT0-0.200913-00="0"iCytoplasmic8.9680.17
ZIPA_ECOLIP77173
zipA
b2412 JW2404 Cell division protein zipAPeriplasmic with N-terminal membrane anchor (Experimental)Has been purified as a complex with ZipA-EptA. PMID: 9008158PMID: 16079137 PMID: 9008158Integral Inner Membrane BBin/outCell inner membrane | Single-pass type I membrane proteinPeriplasmic with N-terminal membrane anchor (Experimental)Soluble protein detected in Inner Membrane FractionSRPSECyesyesNo250.169CYT0-0.200913-11="0"i7-26oCytoplasmicMembrane1096.4
MINE_ECOLIP0A734
minE
b1174 JW1163 Cell division topological specificity factorPeripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature. PMID: 21738659PMID: 20025670 PMID: 10096083 PMID: 21738659Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo180.123CYT0-0.200913-00="0"iCytoplasmic9.97101.97
CVRA_ECOLIP76007
cvrA

ycgO

ycgOb1191 JW5184 Cell volume regulation protein AIntegral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo460.165TMH09.70846-1113="0"o6-23i30-47o59-76i88-113o119-141i162-179o191-210i217-237o243-261i273-292o298-323i335-357o363-381iCytoplasmicMembrane10
BCSA_ECOLIP37653
bcsA

yhjO yhjP

yhjO yhjPb3533 JW5665 Cellulose synthase catalytic subunit [UDP-forming] (EC 2.4.1.12) (Experimental) BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis. 24127606 Cellulose filaments have been visualized in situ via scanning electron and fluorescence microscopy. PMID: 2409795424127606 PMID: 24097954Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo160.123CYT0-0.200913-109Yn3-10c15/16o31-50i150-168o174-190i197-216o228-252i524-547o553-575i639-659o665-690iCytoplasmicMembrane10
BCSC_ECOLIP37650
bcsC

yhjL

yhjLb3530 JW5942 Cellulose synthase operon protein CPeriplasmicGGPeriplasmicN/ASECnoyesYes240.51SpI236.58448-00Yn8-18c23/24oUnknown4.72
WZZB_ECOLIP76372
wzzB

cld rol wzz

cld rol wzzb2027 JW5836 Chain length determinant protein (Polysaccharide antigen chain regulator)Cell inner membrane (Probable) Integral inner membrane (Probable)PMID: 16079137 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECnoyesNo530.174CYT0-0.200913-22="0"o32-51i296-316oCytoplasmicMembrane108.11
CBPM_ECOLIP63264
cbpM

yccD

yccDb0999 JW0984 Chaperone modulatory protein CbpMCytoplasmicAACytoplasmicN/AyesyesNo220.105CYT0-0.200913-00="0"iUnknown235.8
CLPB_ECOLIP63284
clpB

htpM

htpMb2592 JW2573 Chaperone protein ClpB (Heat shock protein F84.1)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental)PMID: 23230279 PMID: 8376377Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo220.119CYT0-0.200913-00="0"iCytoplasmic1075.38
DNAJ_ECOLIP08622
dnaJ

groP

groPb0015 JW0014 Chaperone protein dnaJ (HSP40) (Heat shock protein J)Peripheral inner membrane protein facing the cytoplasm (Experimental) (Experimental) Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 23230279 PMID: 20153451Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo390.105CYT0-0.200913-00="0"iCytoplasmic1052.19
DNAK_ECOLIP0A6Y8
dnaK

groP grpF seg

groP grpF segb0014 JW0013 Chaperone protein dnaK (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) (Experimental)DnaK associates with the inner membrane. The ability to isolate DnaK with a membrane fraction prepared under gentle lysis conditions supports a peripheral association between DnaK and the cytoplasmic membrane. PMID: 7961473 Overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to green fluorescent protein (GFP) led to an increased association (2.3–4.8-fold) of DnaK with the membrane. PMID: 17446557 PMID: 1396676 PMID: 7783627 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 16079137 PMID: 17446557 PMID: 1396676 PMID: 7783627 PMID: 23230279 PMID: 20153451Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo200.127CYT0-0.200913-00="0"iCytoplasmic10115.64
ECPD_ECOLIP33128
ecpD
b0140 JW0136 Chaperone protein ecpDPutative chaperone–usher fimbrial operon (class γ4); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P].PeriplasmicGGSecretedPeriplasmicN/AfimbriaeSECSurAnoyesYes260.667SpI2516.6944-00Yn8-19c25/26oPeriplasmic10
FIMC_ECOLIP31697
fimC
b4316 JW4279 Chaperone protein fimCPeriplasmic (Experimental)Fim operon (class γ1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 1363735PMID: 1363735PeriplasmicGGSecretedPeriplasmic (Experimental)N/AfimbriaeSECSurAyesyesNo370.473SpI3611.9808-10Yn14-23c41/42oPeriplasmic10
HCHA_ECOLIP31658
hchA

yedU yzzC

yedU yzzCb1967 JW1950 Chaperone protein hchA (EcHsp31) (Hsp31)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo280.113CYT0-0.200913-00="0"iCytoplasmic1078.95
HSCA_ECOLIP0A6Z1
hscA

hsc

hscb2526 JW2510 Chaperone protein hscA (Hsc66)CytoplasmicAACytoplasmicN/AyesyesNo220.165CYT0-0.200913-00="0"iCytoplasmic9.97101.91
HSCC_ECOLIP77319
hscC

ybeW

ybeWb0650 JW0645 Chaperone protein hscC (Hsc62)CytoplasmicAACytoplasmicN/AnoyesNo210.135CYT0-0.200913-00="0"iCytoplasmic9.9774.03
HTPG_ECOLIP0A6Z3
htpG
b0473 JW0462 Chaperone protein htpG (Heat shock protein C62.5) (Heat shock protein htpG) (High temperature protein G)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo430.189CYT0-0.200913-00="0"iCytoplasmic10109.49
SFMC_ECOLIP77249
sfmC
b0531 JW0520 Chaperone protein sfmC (Experimental)Potential fimbrial sfm operon (class γ1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Might be is co-translationally translocated through Sec translocon. The precursor of SfmC harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 The precursor of SfmC harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 24717922 PMID: 15838024PeriplasmicGGSecretedPeriplasmicN/AfimbriaeSRP (exp)SECSurAyesyesYes240.844SpI2314.9715-10Yn7-18c23/24oPeriplasmic1028.49
SKP_ECOLIP0AEU7
skp

hlpA ompH

hlpA ompHb0178 JW0173 Chaperone protein skp (DNA-binding 17 kDa protein) (Histone-like protein HLP-1) (Seventeen kilodalton protein)Periplasm (Probable) Periplasmic (Experimental) (Experimental)Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamA. PMID: 2167239 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350PMID: 2318304 PMID: 1838129 PMID: 8730870 PMID: 2167239 PMID: 9298646 PMID: 22030350PeriplasmicGGSecretedPeriplasmic(experimental)N/ASECyesyesYes210.881SpI2022.0906-00Yn4-15c20/21oPeriplasmic1048.54
TORD_ECOLIP36662
torD
b0998 JW0983 Chaperone protein torDCytoplasm (Probable)Trimethylamine N-oxide (TMAO) respiration system is encoded by the torCAD operon and comprises a periplasmic TMAO reductase (TorA) and a c-type cytochrome (TorC), which shuttles electrons to TorA. TorD, a cytoplasmic chaperone that interacts with the unfolded trimethylamine N-oxide reductase enzyme (TorA). The absence of TorD protein leads to a two times decrease of the final amount of TorA enzyme.PMID: 9632735CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo530.154CYT0-0.200913-00="0"iCytoplasmic10133.45
SURA_ECOLIP0ABZ6
surA
b0053 JW0052 Chaperone surA (Peptidyl-prolyl cis-trans isomerase surA) (PPIase surA) (EC 5.2.1.8) (Rotamase surA) (Survival protein A)Periplasmic (Experimental) (Experimental)PMID: 9298646 PMID: 22030350PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes210.795SpI2015.5107-00Yn7-15c20/21oPeriplasmic1086.14
HDEA_ECOLIP0AES9
hdeA

yhhC yhiB

yhhC yhiBb3510 JW3478 Chaperone-like protein hdeA (10K-S protein)Periplasm (Probable) Periplasmic (Experimental) (Experimental)Experimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544PMID: 10623550 PMID: 17085547 PMID: 8455549 PMID: 25403562PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECYfgMyesyesYes220.698SpI2116.385-10Yn4-15c21/22oUnknown2.5108.01
CHEA_ECOLIP07363
cheA
b1888 JW1877 Chemotaxis protein CheA (EC 2.7.13.3)Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicIM (predicted), Soluble IM Fraction +noyesNo170.108CYT0-0.200913-00="0"iCytoplasmic1088.79
CHEW_ECOLIP0A964
cheW
b1887 JW1876 Chemotaxis protein CheWCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo600.102CYT0-0.200913-00="0"iCytoplasmic1081.49
CHEY_ECOLIP0AE67
cheY
b1882 JW1871 Chemotaxis protein CheYCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo370.12CYT0-0.200913-00="0"iCytoplasmic1095.42
CHER_ECOLIP07364
cheR

cheX

cheXb1884 JW1873 Chemotaxis protein methyltransferase (EC 2.1.1.80)CytoplasmicAACytoplasmicN/AyesyesNo440.139CYT0-0.200913-00="0"iCytoplasmic1011.42
CHEB_ECOLIP07330
cheB
b1883 JW1872 Chemotaxis response regulator protein-glutamate methylesterase (EC 3.1.1.61)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo420.119CYT0-0.200913-00="0"iCytoplasmic108.91
BETA_ECOLIP17444
betA
b0311 JW0303 Choline dehydrogenase (CDH) (CHD) (EC 1.1.99.1)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 3512525 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane proteinCytoplasmicN/AYidCnoyesNo190.163CYT0-0.200913-00="0"iCytoplasmicMembrane108.8
AROC_ECOLIP12008
aroC
b2329 JW2326 Chorismate synthase (EC 4.2.3.5) (5-enolpyruvylshikimate-3-phosphate phospholyase)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)yesyesNo250.109CYT0-0.200913-00="0"iCytoplasmic9.9758.16
UBIC_ECOLIP26602
ubiC
b4039 JW5713 Chorismate--pyruvate lyase (CL) (CPL) (EC 4.1.3.40)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo210.105CYT0-0.200913-00="0"iCytoplasmic1066.18
DNAA_ECOLIP03004
dnaA
b3702 JW3679 Chromosomal replication initiator protein dnaAPeripheral inner membrane protein facing the cytoplasm (Experimental) Nucleoid (Experimental)C-terminal amphiphilic helix (355-370). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 10660626 PMID: 23138451 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmCytoplasmicN/AnoyesNo320.146CYT0-0.200913-00="0"iCytoplasmic1038.43
ICIA_ECOLIP0A8S1
iciA
b2916 JW2883 Chromosome initiation inhibitor (OriC replication inhibitor)Nucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AyesyesNo250.133CYT0-0.200913-00="0"iCytoplasmic9.9717.03
MUKB_ECOLIP22523
mukB
b0924 JW0907 Chromosome partition protein mukB (Structural maintenance of chromosome-related protein)Nucleoid (Experimental) Nucleoid (Probable) Cell poles (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 16982609 PMID: 20921377 PMID: 11886550 PMID: 22380631 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1Cytoplasm nucleoidCytoplasmicN/AyesyesNo460.13CYT0-0.200913-00="0"iCytoplasmic10
MUKE_ECOLIP22524
mukE

kicA ycbA

kicA ycbAb0923 JW0906 Chromosome partition protein mukE (Protein kicA)Nucleoid (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 16982609 PMID: 20921377 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1Cytoplasm nucleoidCytoplasmicN/AyesyesNo250.113CYT0-0.200913-00="0"iCytoplasmic9.9785.77
MUKF_ECOLIP60293
mukF

kicB

kicBb0922 JW0905 Chromosome partition protein mukF (Protein kicB)Nucleoid (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 16982609 PMID: 20921377 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1Cytoplasm nucleoidCytoplasmicN/AyesyesNo370.127CYT0-0.200913-00="0"iCytoplasmic1076.09
CINAL_ECOLIP77808
yfaY
b2249 JW2243 CinA-like proteinCytoplasmicAACytoplasmicN/AyesyesNo270.146CYT0-0.200913-00="0"iUnknown252.34
CITT_ECOLIP0AE74
citT

ybdS

ybdSb0612 JW0604 Citrate carrier (Citrate transporter) (Citrate/succinate antiporter)Integral Membrane Protein (Experimental)PMID: 9696764Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECyesyesNo580.164TMH013.6692-1314="0"i7-27o33-50i57-74o94-117i138-155o161-178i190-213o233-259i288-304o310-329i341-361o381-412i424-443o463-486iCytoplasmicMembrane10
CITD_ECOLIP69330
citD

ybdX

ybdXb0617 JW0609 Citrate lyase acyl carrier protein (Citrate lyase gamma chain)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo100.125CYT0-0.200913-00="0"iCytoplasmic1078.86
CILA_ECOLIP75726
citF

ybdV

ybdVb0615 JW5087 Citrate lyase alpha chain (Citrase alpha chain) (EC 4.1.3.6) (Citrate (pro-3S)-lyase alpha chain) (Citrate CoA-transferase subunit) (EC 2.8.3.10)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo270.108CYT0-0.200913-00="0"iCytoplasmic1036.85
CITE_ECOLIP0A9I1
citE

ybdW

ybdWb0616 JW0608 Citrate lyase subunit beta (Citrase beta chain) (EC 4.1.3.6) (Citrate (pro-3S)-lyase subunit beta) (Citryl-CoA lyase subunit) (EC 4.1.3.34)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo260.381CYT0-0.200913-00="0"iCytoplasmic1020.61
CISY_ECOLIP0ABH7
gltA

gluT icdB

gluT icdBb0720 JW0710 Citrate synthase (EC 2.3.3.1)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo430.113CYT0-0.200913-00="0"iCytoplasmic9.9722.15
APHA_ECOLIP0AE22
aphA

napA yjbP

napA yjbPb4055 JW4015 Class B acid phosphatase (EC 3.1.3.2)Periplasm (Probable) Periplasmic (Experimental)PMID: 9011040 PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes260.643SpI2514.5914-00Yn7-17c25/26oUnknown2.5
HSCB_ECOLIP0A6L9
hscB

yfhE

yfhEb2527 JW2511 Co-chaperone protein hscB (Hsc20)CytoplasmicAACytoplasmicN/AyesyesNo580.11CYT0-0.200913-00="0"iCytoplasmic9.26
BTUR_ECOLIP0A9H5
btuR

cobA

cobAb1270 JW1262 Cob(I)yrinic acid a,c-diamide adenosyltransferase (EC 2.5.1.17) (Cob(I)alamin adenosyltransferase) (Corrinoid adenosyltransferase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo450.117CYT0-0.200913-00="0"iCytoplasmic10103.77
COBS_ECOLIP36561
cobS
b1992 JW1970 Cobalamin synthase (EC 2.-.-.-)Cell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo580.15TMH03.98364-46="0"i34-58o64-88i109-132o138-158i178-196o202-219iCytoplasmicMembrane102.51
COABC_ECOLIP0ABQ0
coaBC

dfp

dfpb3639 JW5642 Coenzyme A biosynthesis bifunctional protein CoaBC (DNA/pantothenate metabolism flavoprotein) [Includes: Phosphopantothenoylcysteine decarboxylase (PPCDC) (EC 4.1.1.36) (CoaC); Phosphopantothenate--cysteine ligase (EC 6.3.2.5) (CoaB) (PPC synthetase) (PPCCytoplasmicAACytoplasmicN/AyesyesNo550.128CYT0-0.200913-00="0"iCytoplasmic9.97
WCAK_ECOLIP71242
wcaK
b2045 JW2030 Colanic acid biosynthesis protein wcaK (EC 2.-.-.-)CytoplasmicAACytoplasmicN/AnoyesNo350.188CYT0-0.200913-00="0"iCytoplasmic8.964.27
WCAM_ECOLIP71244
wcaM

yefK

yefKb2043 JW2028 Colanic acid biosynthesis protein wcaMTAT secretion system.PeriplasmicGGPeriplasmicN/ATATnoyesYes290.421SpI2815.7954-00Yn10-21c28/29oUnknown2.5
RCSA_ECOLIP0DMC9
rcsA
b1951 JW1935 Colanic acid capsular biosynthesis activation protein ANucleoid (Experimental) Nucleoid (Potential) LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 23138451 NucleoidNNCytoplasmicN/AnoyesNo280.126CYT0-0.200913-00="0"iUnknown231.55
CSPA_ECOLIP0A9X9
cspA

cspS

cspSb3556 JW3525 Cold shock protein cspA (CSP-A) (7.4 kDa cold shock protein) (CS7.4)Nucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmCytoplasmicN/AnoyesNo200.102CYT0-0.200913-00="0"iCytoplasmic1092.28
CSPB_ECOLIP36995
cspB
b1557 JW1549 Cold shock-like protein cspB (CSP-B)CytoplasmicAACytoplasmCytoplasmicN/AProphage Integrase (EcoGene)nonoNo660.108CYT0-0.200913-00="0"iCytoplasmic9.26114.06
CSPC_ECOLIP0A9Y6
cspC

msmB

msmBb1823 JW1812 Cold shock-like protein cspC (CSP-C)Nucleoid (Experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Cold-shock DNA-binding domain protein (Stress protein, member of the CspA-family). 80222618022261NucleoidNNCytoplasmCytoplasmicN/AnoyesNo260.104CYT0-0.200913-00="0"iCytoplasmic9.2693.85
CSPD_ECOLIP0A968
cspD

cspH ybjA

cspH ybjAb0880 JW0864 Cold shock-like protein cspD (CSP-D)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo620.114CYT0-0.200913-00="0"iCytoplasmic10
CSPE_ECOLIP0A972
cspE

gicA msmC

gicA msmCb0623 JW0618 Cold shock-like protein cspE (CSP-E)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo460.103CYT0-0.200913-00="0"iCytoplasmic9.26
CSPF_ECOLIP0A976
cspF
b1558 JW1550 Cold shock-like protein cspF (CSP-F)CytoplasmicAACytoplasmCytoplasmicN/AProphage Integrase (EcoGene)nonoNo650.108CYT0-0.200913-00="0"iCytoplasmic9.2621
CSPG_ECOLIP0A978
cspG

cspI

cspIb0990 JW0974 Cold shock-like protein cspG (CPS-G)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo660.102CYT0-0.200913-00="0"iCytoplasmic9.26
CSPH_ECOLIP0A982
cspH

cspK

cspKb0989 JW5134 Cold shock-like protein cspH (CSP-H)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo430.109CYT0-0.200913-00="0"iCytoplasmic9.26102.85
CSPI_ECOLIP0A986
cspI

cspJ

cspJb1552 JW1544 Cold shock-like protein cspI (CPS-I)CytoplasmicAACytoplasmCytoplasmicN/AProphage Integrase (EcoGene)nonoNo200.103CYT0-0.200913-00="0"iCytoplasmic9.26
DEAD_ECOLIP0A9P6
deaD

csdA mssB rhlD

csdA mssB rhlDb3162 JW5531 Cold-shock DEAD box protein A (EC 3.6.4.13) (ATP-dependent RNA helicase deaD) (Translation factor W2)Septal ring protein Peripheral inner membrane protein facing the cytoplasm (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasm (Experimental)Trigger factor-sensitive aggregation PMID: 19737520. Contains a SPOR domain. PMID: 8552679PMID: 19880599 PMID: 23230279 PMID: 8552679Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/ATFyesyesNo350.151CYT0-0.200913-00="0"iCytoplasmic1050.36
CIRA_ECOLIP17315
cirA

cir feuA

cir feuAb2155 JW2142 Colicin I receptorInner membrane protein (Experimental) Outer membrane protein (Experimental) (Potential) (Experimental) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 10806384 PMID: 20932056 PMID: 19953543 PMID: 10430870Outer Membrane b-barrel proteinHHSingle-pass type I membrane proteinOuter Membrane β-barrel protein (Experimental)N/ASECDSBA,SKPBAM: β-Barrel Assembly MachineryyesyesYes260.695SpI2511.8271-00Yn10-20c25/26oOuterMembrane1046.98
CVPA_ECOLIP08550
cvpA

dedE

dedEb2313 JW2310 Colicin V production protein (Protein dedE) (Pur regulon 18 kDa protein)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECyesyesNo310.117TMH06.39839-44="0"o6-25i32-52o64-87i99-120oCytoplasmicMembrane10
CUTC_ECOLIP67826
cutC

cutCM

cutCMb1874 JW1863 Copper homeostasis protein cutC Copper ion binding protein CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo230.17CYT0-0.200913-00="0"iCytoplasmic8.9620.15
COPA_ECOLIQ59385
copA

ybaR

ybaRb0484 JW0473 Copper-exporting P-type ATPase A (EC 3.6.3.n1)Integral inner membrane (Experimental) Integral Membrane Protein (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 10639134 Disulfide bond-containing protein PMID: 25664544PMID: 15919996 PMID: 10639134Integral Inner Membrane BBin/inCell membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPYidCnoyesNo500.162CYT0-0.200913-88="0"i185-205o217-238i245-264o284-302i436-458o464-487i778-797o803-824iCytoplasmicMembrane1021.12
HEM6_ECOLIP36553
hemF
b2436 JW2429 Coproporphyrinogen-III oxidase, aerobic (Coprogen oxidase) (Coproporphyrinogenase) (EC 1.3.3.3)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo260.169CYT0-0.200913-00="0"iCytoplasmic10101.63
RUSA_ECOLIP0AG74
rusA

rus ybcP

rus ybcPb0550 JW0538 Crossover junction endodeoxyribonuclease rusA (EC 3.1.22.4) (Holliday junction nuclease rusA) (Holliday junction resolvase)CytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo360.103CYT0-0.200913-00="0"iUnknown275.94
RUVC_ECOLIP0A814
ruvC
b1863 JW1852 Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Holliday junction nuclease ruvC) (Holliday junction resolvase ruvC)Cytoplasm (Probable) Nucleoid (Experimental) Nuclease that resolves Holliday junction intermediates in genetic recombination. 1661673PMID: 21219465 1661673NucleoidNNCytoplasmicN/AyesyesNo650.111CYT0-0.200913-00="0"iCytoplasmic9.9718.49
CAIA_ECOLIP60584
caiA

yaaO

yaaOb0039 JW0038 Crotonobetainyl-CoA dehydrogenase (EC 1.3.99.-) (Crotonobetainyl-CoA reductase)CytoplasmicAACytoplasmicN/AyesyesNo300.118CYT0-0.200913-00="0"iCytoplasmic1041.08
PAAH_ECOLIP76083
paaH

ydbU

ydbUb1395 JW1390 Crotonobetainyl-CoA:carnitine CoA-transferase (EC 2.8.3.-)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo410.145CYT0-0.200913-00="0"iCytoplasmic9.977.35
BGLG_ECOLIP11989
bglG

bglC

bglCb3723 JW3701 Cryptic beta-glucoside bgl operon antiterminatorPeripheral inner membrane protein facing the cytoplasm (Experimental)BglG is a transcriptional antiterminator which controls the expression bgl operon. It has been detected at the membrane when it is expressed with BglF, when expressed as monocistron, the bglG transcripts were observed at the cell poles (unpublished data). In the absence of β-glucosidase, BglG also assembles near membrane and forms a pro-form complex with BglF that maintains BglG inactive. In the presence of sugar, BglG associates with the proteins of the sugar phosphotransfer system (PTS) and localizes at the cell poles. BglG requires two distinct (de)phosphorylation events to gain activity.PMID: 22984181Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AnoyesNo380.103CYT0-0.200913-00="0"iCytoplasmic8.9648.58
BGLH_ECOLIP26218
bglH

yieC

yieCb3720 JW3698 Cryptic outer membrane porin BglHOuter membrane (Probable) Reconstitution experiments with lipid bilayer membranes defined BglH as a channel-forming component, i.e. it is an outer membrane porin. Can be easily removed from the outer membrane by treatment with detergent. PMID: 10027967 PMID: 10027967PMID: 10027967Outer Membrane b-barrel proteinHHCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)N/ASECBAM: β-Barrel Assembly MachinerynoyesYes260.64SpI2513.3498-00Yn6-17c25/26oOuterMembrane108.76
CSGD_ECOLIP52106
csgD
b1040 JW1023 CsgBAC operon transcriptional regulatory proteinNucleoid (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Nucleoid (Potential)Necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. CsgD despite its function as a transcription activator, is localized in the cytoplasmic membrane. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 20874755 PMID: 16513732 PMID: 23138451 Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo260.267CYT0-0.200913-00="0"iCytoplasmic9.2620.1
NUDG_ECOLIP77788
nudG

ynjG

ynjGb1759 JW1748 CTP pyrophosphohydrolase (EC 3.6.1.-)CytoplasmicAACytoplasmicN/AyesyesNo210.113CYT0-0.200913-00="0"iCytoplasmic8.9690.47
PYRG_ECOLIP0A7E5
pyrG
b2780 JW2751 CTP synthase (EC 6.3.4.2) (CTP synthetase) (UTP--ammonia ligase)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)PyrG=CTP synthase or CtpS in some systems. CTP synthase forms filaments in Caulobacter crescentus that regulate cell curvature independently of its catalytic function. The morphogenic role of CtpS requires its functional interaction with the intermediate filament, crescentin (CreS). Trigger factor-sensitive aggregation. PMID: 19737520 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 20639870 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicIM (predicted)TFyesyesNo270.24SpI261.52462-10="0"iCytoplasmic9.9777.31
CSGC_ECOLIP52107
csgC
b1043 JW1026 Curli assembly protein CsgCUniprot mentions an 8aa signal peptide but Signal P prediction is for a "typical" 22 residue peptide Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544PeriplasmicGGSecretedPeriplasmicN/ASECnoyesNo220.179SpI222.07115-00Yn4-16c21/22oUnknown2.568.8
CSGE_ECOLIP0AE95
csgE
b1039 JW1022 Curli production assembly/transport component csgEPeriplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475PMID: 9928475PeriplasmicGGPeriplasmic (Experimental)N/ACurliSECSurAyesyesYes230.789SpI223.89784-00Yn7-17c22/23oUnknown215.08
CSGF_ECOLIP0AE98
csgF
b1038 JW1021 Curli production assembly/transport component csgFOuter membrane protein (Probable) Peripheral outer membrane protein facing the periplasm (Probable) Cell surface appendage (Probable) Periplasmic (Experimental)Curli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475PMID: 16522795 PMID: 21645131 PMID: 19131513 PMID: 9928475Peripheral outer membrane protein facing the extra-cellular spaceF4F4Periplasmic (Experimental)N/ACurliSECCurliyesyesYes200.853SpI1913.9164-10Yn6-14c19/20oExtracellular9.6430.3
CSGG_ECOLIP0AEA2
csgG
b1037 JW1020 Curli production assembly/transport component csgGOuter membrane Lipoprotein (Experimental)Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130PMID: 15174130 PMID: 21645131Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceI, F4I, F4Cell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)N/ACurliSECLOL: Localization Of LipoproteinsyesyesYes200.544SpII1512.9163Pos+2=L00Yn4-13c18/19oCytoplasmicMembrane9.8244.19
CBPA_ECOLIP36659
cbpA
b1000 JW0985 Curved DNA-binding proteinNucleoid (Probable)Copurifies with trigger factor in pull down assays.PMID: 10947847 PMID: 8302830NucleoidNNCytoplasm nucleoidCytoplasmicN/ATFyesyesNo390.103CYT0-0.200913-00="0"iCytoplasmic1061.49
CYNS_ECOLIP00816
cynS

cnt

cntb0340 JW0331 Cyanate hydratase (Cyanase) (EC 4.2.1.104) (Cyanate hydrolase) (Cyanate lyase)CytoplasmicAACytoplasmicN/AnoyesNo580.182CYT0-0.200913-00="0"iUnknown6.4952.43
CYNX_ECOLIP17583
cynX
b0341 JW0332 Cyanate transport protein cynXCell inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCnoyesNo550.147CYT0-0.200913-1211Yn2-10c18/19o34-55i67-85o91-113i125-147o153-173i201-224o230-251i263-281o287-309i321-339o354-372iCytoplasmicMembrane10
GMR_ECOLIP77334
gmr

yciR

yciRb1285 JW1278 Cyclic di-GMP phosphodiesterase Gmr (EC 3.1.4.52)CytoplasmicAACytoplasmicN/AnoyesNo220.115CYT0-0.200913-00="0"iCytoplasmicMembrane7.88
YAHA_ECOLIP21514
yahA
b0315 JW0307 Cyclic di-GMP phosphodiesterase yahA (EC 3.1.4.-)Nucleoid (Experimental) Nucleoid (Potential) LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 23138451 NucleoidNNCytoplasmicN/AyesyesNo280.118CYT0-0.200913-00="0"iCytoplasmicMembrane7.8825.85
YFGF_ECOLIP77172
yfgF
b2503 JW2488 Cyclic di-GMP phosphodiesterase YfgF (EC 3.1.4.52)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo640.246TMH018.0434-99="0"o14-32i44-74o86-106i126-149o169-187i216-235o241-258i265-285o297-316iCytoplasmicMembrane10
YHJH_ECOLIP37646
yhjH
b3525 JW3493 Cyclic di-GMP phosphodiesterase YhjH (EC 3.1.4.52)CytoplasmicAACytoplasmicN/AyesyesNo470.118CYT0-0.200913-00="0"iCytoplasmicMembrane7.886.54
BCSB_ECOLIP37652
bcsB

yhjN

yhjNb3532 JW3500 Cyclic di-GMP-binding protein (Cellulose synthase regulatory subunit)Integral inner membrane (Experimental) (Experimental)BscC is involved in biosynthesis of cellulose, which is a significant constituent of the extracellular matrix. Encodes the catalytic subunit of cellulose synthase. Exhibites aberrant localization by microscopy in the absence of YidC. BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis. 24127606 Cellulose filaments have been visualized in situ via scanning electron and fluorescence microscopy. PMID: 24097954PMID: 15919996 24127606 PMID: 24097954Integral Inner Membrane BBout/inCell inner membrane | Single-pass type I membrane protein.Integral Membrane ProteinIM (predicted)SRPYidCnoyesYes260.342SpI2520.4203-21Yn5-13c25/26o745-766iCytoplasmicMembrane1082.28
CFA_ECOLIP0A9H7
cfa

cdfA

cdfAb1661 JW1653 Cyclopropane-fatty-acyl-phospholipid synthase (CFA synthase) (Cyclopropane fatty acid synthase) (EC 2.1.1.79)Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AYidCyesyesNo380.183CYT0-0.200913-00="0"iCytoplasmic1026.75
YBAK_ECOLIP0AAR3
ybaK
b0481 JW0470 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase ybaKCytoplasmicAACytoplasmCytoplasmicN/AnoyesNo630.121CYT0-0.200913-00="0"iCytoplasmic9.9799.59
METC_ECOLIP06721
metC
b3008 JW2975 Cystathionine beta-lyase metC (CBL) (EC 4.4.1.8) (Beta-cystathionase) (Cysteine lyase)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo420.116CYT0-0.200913-00="0"iCytoplasmic1027.58
METB_ECOLIP00935
metB
b3939 JW3910 Cystathionine gamma-synthase (CGS) (EC 2.5.1.48) (O-succinylhomoserine (thiol)-lyase)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo370.127CYT0-0.200913-00="0"iCytoplasmic1044.96
ISCS_ECOLIP0A6B7
iscS

yfhO yzzO

yfhO yzzOb2530 JW2514 Cysteine desulfurase (EC 2.8.1.7) (NifS protein homolog) (ThiI transpersulfidase)Peripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo480.104CYT0-0.200913-00="0"iCytoplasmic9.9737.46
SUFS_ECOLIP77444
sufS

csdB ynhB

csdB ynhBb1680 JW1670 Cysteine desulfurase (EC 2.8.1.7) (Selenocysteine beta-lyase) (SCL) (Selenocysteine lyase) (EC 4.4.1.16) (Selenocysteine reductase)Peripheral inner membrane protein facing the cytoplasm (Probable)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS probably through direct interaction.PMID: 12941942Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo660.18CYT0-0.200913-00="0"iCytoplasmic1011.17
SUFE_ECOLIP76194
sufE

ynhA

ynhAb1679 JW1669 Cysteine desulfuration protein sufEPeripheral inner membrane protein (Probable)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction .PMID: 12941942Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo660.103CYT0-0.200913-00="0"iCytoplasmic9.26104.06
CSDA_ECOLIQ46925
csdA

ygdJ

ygdJb2810 JW2781 Cysteine sulfinate desulfinase (CSD) (EC 4.4.1.-)Ribosomal (Experimental) Peripheral inner membrane protein facing the cytoplasm (Probable)Assembly cofactor of ribosome; associate with the 50S subunit. Under cold-shock conditions CsdA participates as an alternative DEAD-box RNA helicase together with RNase E in a ‘cold shock’ RNA degradosome (Prud’homme-Genereux et al., 2004). It is therefore possible that CsdA may associate with the inner membrane via RNAseE.PMID: 21529161 PMID: 15554978Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo590.194CYT0-0.200913-00="0"iCytoplasmic9.979.8
CYSK_ECOLIP0ABK5
cysK

cysZ

cysZb2414 JW2407 Cysteine synthase A (CSase A) (EC 2.5.1.47) (O-acetylserine (thiol)-lyase A) (OAS-TL A) (O-acetylserine sulfhydrylase A) (Sulfate starvation-induced protein 5) (SSI5)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo310.116CYT0-0.200913-00="0"iCytoplasmic9.2677.42
CYSM_ECOLIP16703
cysM
b2421 JW2414 Cysteine synthase B (CSase B) (EC 2.5.1.47) (O-acetylserine (thiol)-lyase B) (OAS-TL B) (O-acetylserine sulfhydrylase B)CytoplasmicAACytoplasmicN/AyesyesNo250.12CYT0-0.200913-00="0"iCytoplasmic9.9752.45
EAMB_ECOLIP38101
eamB

yfiK

yfiKb2578 JW2562 Cysteine/O-acetylserine efflux proteinCell inner membrane (Probable) Integral inner membrane (Experimental)PMID: 12562784 PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECnoyesNo450.172TMH07.57291-65Yn4-15c27/28o43-67i74-91o111-133i140-163o175-194iCytoplasmicMembrane10
SYC_ECOLIP21888
cysS
b0526 JW0515 Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA ligase) (CysRS) Trigger factor-sensitive aggregation.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo210.121CYT0-0.200913-00="0"iCytoplasmic9.9740.47
FLIY_ECOLIP0AEM9
fliY

yzzR

yzzRb1920 JW1905 Cystine-binding periplasmic protein (CBP) (Protein fliY) (Sulfate starvation-induced protein 7) (SSI7)Periplasmic (Experimental)PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesNo300.555SpI2910.7798-00Yn10-21c29/30oPeriplasmic1096.19
CDD_ECOLIP0ABF6
cdd
b2143 JW2131 Cytidine deaminase (EC 3.5.4.5) (Cytidine aminohydrolase) (CDA)One very weak transmembrane reg ion pred ict ion. Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicIM (predicted)yesyesNo260.113CYT0-0.200913-00="0"iUnknown242.24
KCY_ECOLIP0A6I0
cmk

mssA ycaF ycaG

mssA ycaF ycaGb0910 JW0893 Cytidylate kinase (CK) (EC 2.7.4.14) (Cytidine monophosphate kinase) (CMP kinase) (Protein mssA) (p25)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo560.152CYT0-0.200913-00="0"iCytoplasmic1086.37
C561_ECOLIP0ABE5
cybB
b1418 JW5224 Cytochrome b561 (Cytochrome b-561)Integral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCnoyesNo230.142TMH07.83021-44="0"i12-33o39-62i82-106o143-162iCytoplasmicMembrane1031.95
C56H_ECOLIP76345
yodB
b1974 JW5323 Cytochrome b561 homolog 1Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo250.146TMH08.67869-44="0"i9-26o46-63i84-108o144-162iCytoplasmicMembrane1015.59
C56I_ECOLIP75925
yceJ
b1057 JW1044 Cytochrome b561 homolog 2Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo310.121TMH013.644-44="0"i12-35o55-72i92-111o144-164iCytoplasmicMembrane1010.58
APPC_ECOLIP26459
appC

cbdA cyxA

cbdA cyxAb0978 JW0960 Cytochrome bd-II oxidase subunit 1 (EC 1.10.3.-) (Cytochrome bd-II oxidase subunit I)Integral inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPYidCyesyesNo370.11TMH011.9815-99="0"o20-42i54-71o91-116i128-145o184-208i215-235o386-409i421-442o471-492iCytoplasmicMembrane10
APPB_ECOLIP26458
appB

cbdB cyxB

cbdB cyxBb0979 JW0961 Cytochrome bd-II oxidase subunit 2 (EC 1.10.3.-) (Cytochrome bd-II oxidase subunit II)Integral inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo410.107TMH010.1949-89="0"o12-40i61-79o85-103i123-148o168-188i209-233o263-283i290-314o334-356iCytoplasmicMembrane10
CCMA_ECOLIP33931
ccmA

yejW

yejWb2201 JW5366 Cytochrome c biogenesis ATP-binding export protein CcmA (EC 3.6.3.41) (Heme exporter protein A)Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo290.16CYT0-0.200913-00="0"iCytoplasmicMembrane7.8847.18
NRFA_ECOLIP0ABK9
nrfA
b4070 JW4031 Cytochrome c-552 (EC 1.7.2.2) (Ammonia-forming cytochrome c nitrite reductase) (Cytochrome c nitrite reductase)Periplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 793493PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes270.665SpI2614.744-00Yn11-21c26/27oPeriplasmic1025.45
CCME_ECOLIP69490
ccmE

yejS

yejSb2197 JW2185 Cytochrome c-type biogenesis protein CcmE (Cytochrome c maturation protein E) (Heme chaperone CcmE)Periplasmic with N-terminal membrane anchor (Experimental) CcmC, CcmE, and heme interact directly with each other, establishing a periplasmic heme delivery pathway for cytochrome c maturation. PMID: 11384983 PMID: 9770295 PMID: 16364305PMID: 9770295 PMID: 16364305Integral Inner Membrane BBin/outCell inner membrane | Single-pass type II membrane protein | Periplasmic sidePeriplasmic with N-terminal membrane anchor (Experimental)N/ASRPSECyesyesNo290.181TMH08.60358-11="0"i9-30oCytoplasmic8.9666.48
CCMF_ECOLIP33927
ccmF

yejR

yejRb2196 JW2184 Cytochrome c-type biogenesis protein CcmFIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo540.151TMH012.9934-1515="0"o6-29i41-62o96-114i121-142o178-198i210-230o250-267i274-294o314-332i353-374o394-412i424-444o450-472i484-503o609-629iCytoplasmicMembrane10
CCMH_ECOLIP0ABM9
ccmH

yejP

yejPb2194 JW2182 Cytochrome c-type biogenesis protein CcmHIntegral inner membrane (Experimental) (Experimental)Might be is co-translationally translocated through Sec translocon. CcmH is a periplasm-resident oxidoreductase anchored to the inner membrane by two transmembrane segments. Both N- and C-terminal domains are located and function in the periplasmic compartment. PMID: 22789558 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 15919996 PMID: 15838024Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRP (exp)SECyesyesYes190.795SpI188.87226-32Yn3-13c18/19o102-120i147-166oCytoplasmicMembrane1041.31
NRFE_ECOLIP32710
nrfE

yjcL

yjcLb4074 JW4035 Cytochrome c-type biogenesis protein nrfECell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo100.193TMH07.53708-1212="0"i21-54o79-97i104-126o158-178i190-212o232-248i260-288o294-312i333-357o377-394i401-422o528-545iCytoplasmicMembrane10
NAPC_ECOLIP0ABL5
napC

yejX

yejXb2202 JW2190 Cytochrome c-type protein napCPeriplasm (Experimental) Inner membrane (Probable) Periplasmic with N-terminal membrane anchor (Experimental) Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 Electrons are fed into the NapAB dimer from a membrane-anchored tetra-heme cytochrome c,encoded by the napC gene. PMID: 8830238 PMID: 12186631PMID: 11844760 PMID: 8830238 PMID: 12186631Integral Inner Membrane BBin/outCell inner membrane | Single-pass membrane proteinPeriplasmic with N-terminal membrane anchor (Experimental)IM (predicted)SRPSECyesyesNo520.116TMH06.01984-11="0"i21-44oCytoplasmicMembrane1014.18
NRFB_ECOLIP0ABL1
nrfB

yjcI

yjcIb4071 JW4032 Cytochrome c-type protein nrfBPeriplasmic (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 15280383PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes330.498SpI256.62799-00Yn7-18c23/24oPeriplasmic9.8416.12
TORC_ECOLIP33226
torC
b0996 JW0981 Cytochrome c-type protein torCPeriplasmic with N-terminal membrane anchor Single-pass type II membrane protein ( Experimental)TAT secretion system. Binds 5 heme groups per subunit ; Belongs to the torC/torY family. Trimethylamine N-oxide (TMAO) respiration system is encoded by the torCAD operon and comprises a periplasmic TMAO reductase (TorA) and a c-type cytochrome (TorC), which shuttles electrons to TorA. The immature C-terminal domain of TorC strongly down-regulated the tor operon and interacted with the TorS detector region. Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase PMID: 11056172PMID: 11056172Integral Inner Membrane BBin/outCell inner membrane | Single-pass type II membrane proteinPeriplasmic with N-terminal membrane anchor (Experimental)N/ASRPSECyesyesNo200.12TMH06.50112-11="0"i12-34oCytoplasmicMembrane9.8243.43
TORY_ECOLIP52005
torY

yecK

yecKb1873 JW1862 Cytochrome c-type protein torYIntegral inner membrane (By similarity) Periplasmic with N-terminal membrane anchor (Experimental) Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorZ. Required for electron transfer to the TorZ terminal enzyme. PMID: 11004177 PMID: 11004177Integral Inner Membrane BBin/outCell inner membrane | Single-pass type II membrane proteinPeriplasmic with N-terminal membrane anchor (Experimental)N/ASRPSECyesyesNo320.123SpI267.35562-10Yn7-18c26/27oUnknown4.9344.83
CYDA_ECOLIP0ABJ9
cydA

cyd-1

cyd-1b0733 JW0722 Cytochrome d ubiquinol oxidase subunit 1 (EC 1.10.3.-) (Cytochrome bd-I oxidase subunit I) (Cytochrome d ubiquinol oxidase subunit I)Cell inner membrane (Probable) Inner membrane (Experimental) Integral inner membrane (Experimental)Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 15013751 PMID: 16079137 PMID: 15919657 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRP (exp)SECyesyesNo370.106TMH09.54728-99="0"o20-42i54-71o91-116i128-151o184-208i220-238o388-409i421-444o471-494iCytoplasmicMembrane10
CYDB_ECOLIP0ABK2
cydB

cyd-2

cyd-2b0734 JW0723 Cytochrome d ubiquinol oxidase subunit 2 (EC 1.10.3.-) (Cytochrome bd-I oxidase subunit II) (Cytochrome d ubiquinol oxidase subunit II)Cell inner membrane (Probable) Integral inner membrane (Experimental)Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion.PMID: 15013751 PMID: 16079137 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRP (exp)SECyesyesNo290.118TMH010.5085-88="0"i9-28o77-99i119-141o161-181i202-226o264-283i290-315o335-360iCytoplasmicMembrane10
CYOD_ECOLIP0ABJ6
cyoD
b0429 JW0419 Cytochrome o ubiquinol oxidase protein cyoD (Ubiquinol oxidase chain D)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECnoyesNo440.15TMH014.0581-33="0"o18-37i44-68o80-100iCytoplasmicMembrane10
CYOC_ECOLIP0ABJ3
cyoC
b0430 JW0420 Cytochrome o ubiquinol oxidase subunit 3 (EC 1.10.3.-) (Cytochrome o ubiquinol oxidase subunit III) (Ubiquinol oxidase chain C)Integral inner membrane (Experimental)PMID: 11017202 PMID: 15919996Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPSECyesyesNo140.175TMH03.44396-55="0"i29-50o70-91i98-118o138-165i177-201oCytoplasmicMembrane10
AMY2_ECOLIP26612
amyA

yedC

yedCb1927 JW1912 Cytoplasmic alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo280.136CYT0-0.200913-00="0"iCytoplasmic1069.39
TREF_ECOLIP62601
treF
b3519 JW3487 Cytoplasmic trehalase (EC 3.2.1.28) (Alpha,alpha-trehalase) (Alpha,alpha-trehalose glucohydrolase)Cytoplasm (Probable) Cytoplasmic (Experimental)PMID: 10816581 PMID: 8892826CytoplasmicAACytoplasmCytoplasmic (Experimental)N/AyesyesNo320.101CYT0-0.200913-00="0"iCytoplasmic1048.63
CODA_ECOLIP25524
codA
b0337 JW0328 Cytosine deaminase (EC 3.5.4.1) (Cytosine aminohydrolase)CytoplasmicAACytoplasmicN/AnoyesNo490.104CYT0-0.200913-00="0"iCytoplasmic9.9711.3
CODB_ECOLIP0AA82
codB
b0336 JW0327 Cytosine permeaseIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPYidCnoyesNo380.154TMH012.7134-1212="0"i20-40o52-74i95-118o130-147i152-173o193-212i224-253o259-278i299-318o324-345i357-379o385-402iCytoplasmicMembrane10
RODZ_ECOLIP27434
rodZ

yfgA

yfgAb2516 JW2500 Cytoskeleton protein rodZNucleoid (Experimental) Inner Membrane; Cytoskeleton (Probable) Integral inner membrane (Potential) Cytoskeleton Integral inner membrane (Probable) Inner membrane (Experimental) (Experimental)Signal recognition particle is required for insertion in the bacterial inner membrane. SRP pathway inhibition blocks membrane insertion. Quantitative immunoblotting agaist RodZ purified protein indicate that RodZ is present at ~650 copies per average exponentially growing cell in LB medium and M9. The hth domain of RodZ is not required for transcription but for interaction with MreB. The protein, RodZ, is required for assembly of the actin cytoskeleton MreB that controls cell wall synthesis and cell shape. PMID: 19194484PMID: 23138451 PMID: 19078962 PMID: 21890895 PMID: 19008860 PMID: 15919657 PMID: 19194484Nucleoid, Integral Inner Membrane N, BN,BCell inner membrane | Single-pass type II membrane proteinMembrane anchoredIM (predicted)SECyesyesNo350.243CYT0-0.200913-11="0"i112-133oUnknown2
AMPA_ECOLIP68767
pepA

carP xerB

carP xerBb4260 JW4217 Cytosol aminopeptidase (EC 3.4.11.1) (Aminopeptidase A/I) (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo350.112CYT0-0.200913-00="0"iCytoplasmic9.9758.35
GMHB_ECOLIP63228
gmhB

yaeD

yaeDb0200 JW0196 D,D-heptose 1,7-bisphosphate phosphatase (EC 3.1.3.-) (D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo600.122CYT0-0.200913-00="0"iCytoplasmic9.9792.65
SERA_ECOLIP0A9T0
serA
b2913 JW2880 D-3-phosphoglycerate dehydrogenase (PGDH) (EC 1.1.1.95)CytoplasmicAACytoplasmicN/AyesyesNo300.107CYT0-0.200913-00="0"iCytoplasmic9.9760.79
DDLA_ECOLIP0A6J8
ddlA
b0381 JW0372 D-alanine--D-alanine ligase A (EC 6.3.2.4) (D-Ala-D-Ala ligase A) (D-alanylalanine synthetase A)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo310.109CYT0-0.200913-00="0"iCytoplasmic1092.36
DDLB_ECOLIP07862
ddlB

ddl

ddlb0092 JW0090 D-alanine--D-alanine ligase B (EC 6.3.2.4) (D-Ala-D-Ala ligase B) (D-alanylalanine synthetase B)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo250.174CYT0-0.200913-00="0"iCytoplasmic1096.97
DACA_ECOLIP0AEB2
dacA

pfv

pfvb0632 JW0627 D-alanyl-D-alanine carboxypeptidase dacA (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Beta-lactamase) (EC 3.5.2.6) (Penicillin-binding protein 5) (PBP-5)Peripheral inner membrane protein facing the cytoplasm (Experimental) Membrane anchored (Experimental) Peripheral inner membrane protein facing the periplasm (Experimental)Anchored to inner membrane by C-terminal tailPMID: 2194801. Using natural isotope abundance NMR, the structure of the PBP 5 anchor peptide within a micelle was determinedPMID: 20192190. The structure conforms to a helix-bend-helix-turn-helix motif and reveals that the anchor enters the membrane so as to form an amphiphilic structure within the interface of the hydrophilic/hydrophobic boundary regions near the lipid head groups. PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones PMID: 20545860. PMID: 3279397 PMID: 8424800 DacA(PBP-5) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. A pH dependent loss of alpha-helical conformation in the peptide corresponding to the PBP5 C-terminus was found to correlate with the susceptibility of PBP5 to membrane extraction. PMID: 9371419PMID: 2194801 PMID: 20192190 PMID: 20545860 PMID: 3279397 PMID: 8424800 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2F2Cell inner membrane | Peripheral membrane protein | Cytoplasmic sideMembrane anchored (Experimental)Soluble protein detected in Inner Membrane FractionSECyesyesYes300.737SpI2917.6151-00Yn13-24c29/30oCytoplasmicMembrane9.978.45
DACB_ECOLIP24228
dacB
b3182 JW3149 D-alanyl-D-alanine carboxypeptidase dacB (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (D-alanyl-D-alanine endopeptidase) (DD-endopeptidase) (EC 3.4.21.-) (Penicillin-binding protein 4) (PBP-4)Peripheral inner membrane protein facing the periplasm (Experimental) Outer membrane protein (Experimental) Membrane anchored (Experimental)Exported from cytosol and signal peptide is cleaved. Anchored via C terminal the rest of the protein protudes into periplasm. Involved in peptidoglycan biosynthesis and is Penicillin-binding. PMID: 16411754 PMID: 15576782 DacB(PBP-4) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. PMID: 9371419PMID: 12444970 PMID: 15911532 PMID: 16411754 PMID: 15576782 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2F2SecretedMembrane anchored (Experimental)N/ASECyesyesYes210.58SpI2013.4163-00Yn6-16c20/21oPeriplasmic9.7634.4
DACC_ECOLIP08506
dacC
b0839 JW0823 D-alanyl-D-alanine carboxypeptidase dacC (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Penicillin-binding protein 6) (PBP-6)Peripheral inner membrane protein facing the periplasm (Potential) Membrane anchored (Experimental) Peripheral inner membrane protein facing the periplasm (Experimental)Peripheral inner membrane protein facing the periplasm. PMID: 9858669 DacC(PBP-6) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. PMID: 9371419PMID: 8162192 PMID: 9858669 PMID: 9371419Peripheral inner membrane protein facing the periplasmF2F2Cell inner membrane | Peripheral membrane proteinMembrane anchored (Experimental)Soluble protein detected in Inner Membrane FractionSECyesyesYes280.887SpI2718.4084-00Yn11-22c27/28oCytoplasmicMembrane9.9721.34
DACD_ECOLIP33013
dacD

phsE yeeC

phsE yeeCb2010 JW5329 D-alanyl-D-alanine carboxypeptidase dacD (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Penicillin-binding protein 6b) (PBP-6b)Membrane anchored (Experimental)Penicillin-binding protein 5 (PBP 5); membrane-bound cell wall dd-carboxypeptidase, localized in the outer leaflet of the cytosolic membrane. PMID: 8955390PMID: 8955390Peripheral inner membrane protein facing the periplasmF2F2Cell inner membrane | Peripheral membrane proteinMembrane anchored (Experimental)N/ASECyesyesYes220.485SpI2112.6922-00Yn5-16c21/22oCytoplasmicMembrane9.9912.62
DDPX_ECOLIP77790
ddpX

vanX yddT

vanX yddTb1488 JW1483 D-alanyl-D-alanine dipeptidase (D-Ala-D-Ala dipeptidase) (EC 3.4.13.22)Cytoplasm (Probable)PMID: 10500118CytoplasmicAACell outer membraneCytoplasmicN/AnoyesNo520.159CYT0-0.200913-00="0"iCytoplasmic9.9745.71
PBP7_ECOLIP0AFI5
pbpG

yohB

yohBb2134 JW5355 D-alanyl-D-alanine endopeptidase (DD-endopeptidase) (EC 3.4.21.-) (Penicillin-binding protein 7) (PBP-7)Periplasmic (Experimental)PMID: 7721700PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes260.58SpI2517.1021-00Yn6-17c25/26oPeriplasmic1082.72
ALSA_ECOLIP32721
alsA

yjcW

yjcWb4087 JW4048 D-allose import ATP-binding protein AlsA (EC 3.6.3.17)Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo210.291CYT0-0.200913-00="0"iCytoplasmicMembrane1018.1
ALSK_ECOLIP32718
alsK

yjcT

yjcTb4084 JW5724 D-allose kinase (Allokinase) (EC 2.7.1.55)CytoplasmicAACytoplasmicN/AnoyesNo250.107CYT0-0.200913-00="0"iCytoplasmic9.9730.51
ALSC_ECOLIP32720
alsC

yjcV

yjcVb4086 JW4047 D-allose transport system permease protein AlsCIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCnoyesNo490.11TMH04.98579-98="0"i21-42o71-93i105-129o168-193i222-246o252-272i279-298o304-324iCytoplasmicMembrane10
ALSB_ECOLIP39265
alsB

yjcX

yjcXb4088 JW4049 D-allose-binding periplasmic protein (ALBP)Periplasmic (Experimental)PMID: 14499611PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes240.81SpI2318.079-10Yn8-18c23/24oPeriplasmic1053.3
ALSE_ECOLIP32719
alsE

yjcU

yjcUb4085 JW4046 D-allulose-6-phosphate 3-epimerase (EC 5.1.3.-)CytoplasmicAACytoplasmicN/AnoyesNo270.112CYT0-0.200913-00="0"iCytoplasmic9.9775.65
DADA_ECOLIP0A6J5
dadA

dadR

dadRb1189 JW1178 D-amino acid dehydrogenase small subunit (EC 1.4.99.1)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 6102989 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionTFYidCyesyesNo230.155TMH00.264511-00Yn3-11c16/17oCytoplasmicMembrane1012.21
DCYD_ECOLIP76316
dcyD

yedO

yedOb1919 JW5313 D-cysteine desulfhydrase (EC 4.4.1.15)Cytoplasmic (Experimental)PMID: 11527960CytoplasmicAACytoplasmic(experimental)N/AnoyesNo490.104CYT0-0.200913-00="0"iCytoplasmic109.95
FOLX_ECOLIP0AC19
folX
b2303 JW2300 D-erythro-7,8-dihydroneopterin triphosphate epimerase (EC 5.-.-.-) (Dihydroneopterin triphosphate 2'-epimerase)CytoplasmicAACytoplasmicN/AyesyesNo460.107CYT0-0.200913-00="0"iCytoplasmic8.9689.59
E4PD_ECOLIP0A9B6
epd

gapB

gapBb2927 JW2894 D-erythrose-4-phosphate dehydrogenase (E4PDH) (EC 1.2.1.72)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo210.147CYT0-0.200913-00="0"iCytoplasmic1031.75
GARD_ECOLIP39829
garD

yhaG

yhaGb3128 JW3097 D-galactarate dehydratase (GalcD) (EC 4.2.1.42)CytoplasmicAACytoplasmicN/AnoyesNo360.116CYT0-0.200913-00="0"iCytoplasmic8.9648.35
DGOD_ECOLIQ6BF17
dgoD

yidU

yidUb4478 JW5629 D-galactonate dehydratase (GalD) (EC 4.2.1.6)CytoplasmicAACytoplasmicN/AnoyesNo440.134CYT0-0.200913-00="0"iCytoplasmic8.964.43
DGOT_ECOLIP0AA76
dgoT

yidT

yidTb3691 JW5859 D-galactonate transporterIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo430.138TMH04.18214-1112="0"i15-35o55-75i82-98o104-122i142-163o169-188i242-263o283-304i316-332o338-362i369-392o404-424iCytoplasmicMembrane10
LDHD_ECOLIP52643
ldhA

hslI htpH

hslI htpHb1380 JW1375 D-lactate dehydrogenase (D-LDH) (EC 1.1.1.28) (Fermentative lactate dehydrogenase)CytoplasmicAACytoplasmicN/AyesyesNo420.109CYT0-0.200913-00="0"iCytoplasmic9.9772.48
DLD_ECOLIP06149
dld
b2133 JW2121 D-lactate dehydrogenase (EC 1.1.1.28) (Respiratory D-lactate dehydrogenase)Inner membrane (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Requires phospholipid for maximal activity. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicSoluble protein detected in Inner Membrane FractionYidCyesyesNo330.121CYT0-0.200913-00="0"iCytoplasmicMembrane1078.9
DMLA_ECOLIP76251
dmlA

yeaU

yeaUb1800 JW1789 D-malate dehydrogenase [decarboxylating] (EC 1.1.1.83) (D-malate degradation protein A) (D-malate oxidase)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo400.107CYT0-0.200913-00="0"iCytoplasmic9.9711.03
UXUB_ECOLIP39160
uxuB
b4323 JW4286 D-mannonate oxidoreductase (EC 1.1.1.57) (Fructuronate reductase)CytoplasmicAACytoplasmicN/AyesyesNo390.139CYT0-0.200913-00="0"iCytoplasmic9.9752.52
METI_ECOLIP31547
metI

yaeE

yaeEb0198 JW0194 D-methionine transport system permease protein metIIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo320.111TMH018.923-55="0"o20-40i52-76o82-105i142-162o182-203iCytoplasmicMembrane10
METQ_ECOLIP28635
metQ

yaeC

yaeCb0197 JW0193 D-methionine-binding lipoprotein metQLipoprotein (Experimental) Inner membrane lipoprotein (Experimental) Proven pignal peptidase II substrate. PMID: 12218041PMID: 15174130 PMID: 12218041Inner Membrane LipoproteinEECell membrane | Lipid-anchorInner membrane lipoprotein (Experimental)Soluble protein detected in Inner Membrane FractionSECLOL: Localization Of LipoproteinsyesyesNo250.18SpII2219.6725Pos+2=G00Yn7-18c23/24oCytoplasmicMembrane9.9785.92
PHYDA_ECOLIQ46806
hyuA

ygeZ

ygeZb2873 JW2841 D-phenylhydantoinase (EC 3.5.2.-) (Hydantoin-utilizing enzyme hyuA)CytoplasmicAACytoplasmicN/AyesyesNo140.108CYT0-0.200913-00="0"iCytoplasmic9.9723.97
LSRR_ECOLIP76141
lsrR

ydeW

ydeWb1512 JW1505 D-ribose pyranase (EC 5.5.1.n1)Nucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmCytoplasmicN/AnoyesNo340.103CYT0-0.200913-00="0"iUnknown219.47
RBSB_ECOLIP02925
rbsB

prlB rbsP

prlB rbsPb3751 JW3730 D-ribose-binding periplasmic proteinPeriplasmic (Probable) Periplasmic (Experimental) Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 PMID: 9298646PMID: 15911532 PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)Soluble protein detected in Inner Membrane FractionSECSkpnoyesYes260.839SpI2522.7093-00Yn6-17c25/26oPeriplasmic1093.52
SDHD_ECOLIP00926
dsdA
b2366 JW2363 D-serine dehydratase (EC 4.3.1.18) (D-serine deaminase) (DSD)CytoplasmicAACytoplasmicN/AnoyesNo370.142CYT0-0.200913-00="0"iCytoplasmicMembrane8.4666.98
CYCA_ECOLIP0AAE0
cycA

dagA ytfD

dagA ytfDb4208 JW4166 D-serine/D-alanine/glycine transporterIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPYidCyesyesNo530.108TMH03.35425-1212="0"i28-45o51-71i108-130o136-154i166-187o207-231i252-274o286-311i347-365o371-397i417-435o441-460iCytoplasmicMembrane10
GATY_ECOLIP0C8J6
gatY

yegF

yegFb2096 JW5343 D-tagatose-1,6-bisphosphate aldolase subunit gatY (TBPA) (TagBP aldolase) (EC 4.1.2.40) (D-tagatose-bisphosphate aldolase class II) (Tagatose-bisphosphate aldolase)Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo490.137CYT0-0.200913-00="0"iCytoplasmic9.9711.46
GATZ_ECOLIP0C8J8
gatZ
b2095 JW2082 D-tagatose-1,6-bisphosphate aldolase subunit gatZPeripheral inner membrane protein facing the cytoplasm (Experimental)Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo330.318CYT0-0.200913-00="0"iCytoplasmic8.966.6
KBAY_ECOLIP0AB74
kbaY

agaY kba yraC

agaY kba yraCb3137 JW3106 D-tagatose-1,6-bisphosphate aldolase subunit kbaY (TBPA) (TagBP aldolase) (EC 4.1.2.40) (D-tagatose-bisphosphate aldolase class II) (Ketose 1,6-bisphosphate aldolase class II) (Tagatose-bisphosphate aldolase)CytoplasmicAACytoplasmicN/AyesyesNo490.226CYT0-0.200913-00="0"iCytoplasmic9.9714.48
KBAZ_ECOLIP0C8K0
kbaZ

agaZ yhaX

agaZ yhaXb3132 JW3101 D-tagatose-1,6-bisphosphate aldolase subunit kbaZCytoplasmicAACytoplasmicN/AyesyesNo400.224CYT0-0.200913-00="0"iCytoplasmic8.9661.59
DTD_ECOLIP0A6M4
dtd

yihZ

yihZb3887 JW3858 D-tyrosyl-tRNA(Tyr) deacylase (EC 3.1.-.-)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo220.108CYT0-0.200913-00="0"iUnknown237.94
XYLF_ECOLIP37387
xylF

xylT

xylTb3566 JW3538 D-xylose-binding periplasmic proteinPeriplasmic (Experimental)PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes240.78SpI2314.4657-00Yn6-17c23/24oPeriplasmic10
XYLE_ECOLIP0AGF4
xylE
b4031 JW3991 D-xylose-proton symporter (D-xylose transporter)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo300.155TMH06.47202-1211Yn13-24c32/33o56-78i90-109o129-151i163-186o198-220i270-293o313-334i346-364o370-395i407-426o446-463iCytoplasmicMembrane10
THIH_ECOLIP30140
thiH
b3990 JW3953 Dehydroglycine synthase (EC 1.3.99.n2) (Tyrosine lyase)CytoplasmicAACytoplasmicN/AyesyesNo260.141CYT0-0.200913-00="0"iUnknown2
HEM2_ECOLIP0ACB2
hemB

ncf

ncfb0369 JW0361 Delta-aminolevulinic acid dehydratase (ALAD) (ALADH) (EC 4.2.1.24) (Porphobilinogen synthase)CytoplasmicAACytoplasmicN/AyesyesNo200.106CYT0-0.200913-00="0"iCytoplasmic9.976.87
DCD_ECOLIP28248
dcd

dus paxA

dus paxAb2065 JW2050 Deoxycytidine triphosphate deaminase (dCTP deaminase) (EC 3.5.4.13)CytoplasmicAACytoplasmicN/AyesyesNo280.106CYT0-0.200913-00="0"iCytoplasmic9.9790.88
DGTP_ECOLIP15723
dgt
b0160 JW0156 Deoxyguanosinetriphosphate triphosphohydrolase (dGTP triphosphohydrolase) (dGTPase) (EC 3.1.5.1)CytoplasmicAACytoplasmicN/AyesyesNo30.105CYT0-0.200913-00="0"iCytoplasmic9.974.2
PHR_ECOLIP00914
phrB

phr

phrb0708 JW0698 Deoxyribodipyrimidine photo-lyase (EC 4.1.99.3) (DNA photolyase) (Photoreactivating enzyme)NucleoidNNCytoplasmicIM (predicted)yesyesNo340.189CYT0-0.200913-00="0"iCytoplasmic8.962.49
TATD_ECOLIP27859
tatD

mttC yigW yigX

mttC yigW yigXb4483 JW5931 Deoxyribonuclease tatD (DNase tatD) (EC 3.1.21.-)Cytoplasm (Probable) Cytoplasmic (Experimental)PMID: 10747959 PMID: 9298646CytoplasmicAACytoplasmCytoplasmic (Experimental)N/Apseudogene (Ochman & Davalos, 2006)noyesNo150.109CYT0-0.200913-00="0"iCytoplasmic10
DEOR_ECOLIP0ACK5
deoR

nucR

nucRb0840 JW0824 Deoxyribose operon repressorNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AyesyesNo660.108CYT0-0.200913-00="0"iCytoplasmic9.9729.23
DEOC_ECOLIP0A6L0
deoC

dra thyR

dra thyRb4381 JW4344 Deoxyribose-phosphate aldolase (EC 4.1.2.4) (Phosphodeoxyriboaldolase) (DERA) (Deoxyriboaldolase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo220.136CYT0-0.200913-00="0"iCytoplasmic10115.23
DUT_ECOLIP06968
dut

dnaS sof

dnaS sofb3640 JW3615 Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP pyrophosphatase)CytoplasmicAACytoplasmicN/AyesyesNo650.192CYT0-0.200913-00="0"iUnknown297.26
COAE_ECOLIP0A6I9
coaE

yacE

yacEb0103 JW0100 Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A kinase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo220.296CYT0-0.200913-00="0"iCytoplasmic9.2663.64
BIOD1_ECOLIP13000
bioD1
b0778 JW0761 Dethiobiotin synthetase (EC 6.3.3.3) (DTB synthetase) (DTBS) (Dethiobiotin synthase)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo360.141CYT0-0.200913-00="0"iCytoplasmic1027.11
KDGL_ECOLIP0ABN1
dgkA
b4042 JW4002 Diacylglycerol kinase (DAGK) (EC 2.7.1.107) (Diglyceride kinase) (DGK)Integral inner membrane (Experimental) Integral Membrane Protein (Experimental)PMID: 15919996 PMID: 2984194 PMID: 8071224Integral Inner Membrane BBin/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted), Detected in IM FractionSRPSECyesyesNo540.112CYT0-0.200913-23="0"i30-51o57-80i100-121oCytoplasmicMembrane1031.25
DCDA_ECOLIP00861
lysA
b2838 JW2806 Diaminopimelate decarboxylase (DAP decarboxylase) (EC 4.1.1.20)CytoplasmicAACytoplasmicN/AyesyesNo550.117CYT0-0.200913-00="0"iCytoplasmic9.9767.45
DAPF_ECOLIP0A6K1
dapF
b3809 JW5592 Diaminopimelate epimerase (DAP epimerase) (EC 5.1.1.7) Disulfide bridge (73 217) CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo190.106CYT0-0.200913-00="0"iCytoplasmic9.9717.3
DOSC_ECOLIP0AA89
dosC

yddV

yddVb1490 JW5241 Diguanylate cyclase DosC (DGC) (EC 2.7.7.65)CytoplasmicAACytoplasmicN/AnoyesNo280.15CYT0-0.200913-00="0"iCytoplasmic9.9720.82
YDEH_ECOLIP31129
ydeH

ydeG

ydeGb1535 JW1528 Diguanylate cyclase YdeH (DGC) (EC 2.7.7.65) Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AyesyesNo240.128CYT0-0.200913-00="0"iCytoplasmic9.9736.55
NAPB_ECOLIP0ABL3
napB

yejY

yejYb2203 JW5367 Diheme cytochrome c napBPeriplasm (Probable) Periplasm (Experimental) Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 10234835 PMID: 10548535 PMID: 17130127 PMID: 11844760PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes280.825SpI2714.6891-00Yn9-20c27/28oPeriplasmic10
DAPB_ECOLIP04036
dapB
b0031 JW0029 Dihydrodipicolinate reductase (DHPR) (EC 1.3.1.26)CytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo250.144CYT0-0.200913-00="0"iCytoplasmic1094.23
DAPA_ECOLIP0A6L2
dapA
b2478 JW2463 Dihydrodipicolinate synthase (DHDPS) (EC 4.2.1.52)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo220.116CYT0-0.200913-00="0"iCytoplasmic10
DYR_ECOLIP0ABQ4
folA

tmrA

tmrAb0048 JW0047 Dihydrofolate reductase (EC 1.5.1.3)CytoplasmicAACytoplasmicN/AyesyesNo200.243CYT0-0.200913-00Yn2-10c15/16oCytoplasmic8.9680.97
FOLM_ECOLIP0AFS3
folM

ydgB

ydgBb1606 JW1598 Dihydrofolate reductase folM (DHFR) (EC 1.5.1.3)CytoplasmicAACytoplasmicN/AyesyesNo240.135CYT0-0.200913-00="0"iCytoplasmic8.9620.39
DLDH_ECOLIP0A9P0
lpdA

lpd

lpdb0116 JW0112 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes) (Glycine cleavage system L protein)Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 6352260 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (45 50) Disulfide bond-containing protein PMID: 25664544PMID: 16079137 PMID: 6352260 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell inner membrane | Peripheral membrane proteinPeripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo230.176CYT0-0.200913-00="0"iCytoplasmic1079.07
ODP2_ECOLIP06959
aceF
b0115 JW0111 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (E2)Inner membrane protein (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Inner membrane (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15911532 PMID: 24742683 PMID: 15919657 PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFnoyesNo520.106CYT0-0.200913-00="0"iCytoplasmic1095.06
ODO2_ECOLIP0AFG6
sucB
b0727 JW0716 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) (2-oxoglutarate dehydrogenase complex component E2) (OGDC-E2) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase cPeripheral inner membrane protein facing the cytoplasm (Experimental) Inner membrane (Experimental) Cytoplasmic (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. PMID: 9298646PMID: 23230279 PMID: 15919657 PMID: 9298646Peripheral inner membrane protein facing the cytoplasmF1F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo190.112CYT0-0.200913-00="0"iCytoplasmic9.97122.42
FOLB_ECOLIP0AC16
folB

ygiG

ygiGb3058 JW3030 Dihydroneopterin aldolase (DHNA) (EC 4.1.2.25)CytoplasmicAACytoplasmicN/AyesyesNo200.108CYT0-0.200913-00="0"iCytoplasmic9.26111.25
NUDB_ECOLIP0AFC0
nudB

ntpA

ntpAb1865 JW1854 Dihydroneopterin triphosphate pyrophosphatase (EC 3.6.1.-) (dATP pyrophosphohydrolase)CytoplasmicAACytoplasmicN/AyesyesNo270.105CYT0-0.200913-00="0"iUnknown252.92
PYRC_ECOLIP05020
pyrC
b1062 JW1049 Dihydroorotase (DHOase) (EC 3.5.2.3)CytoplasmicAACytoplasmicN/AyesyesNo560.167CYT0-0.200913-00="0"iCytoplasmic9.973.92
PYRD_ECOLIP0A7E1
pyrD
b0945 JW0928 Dihydroorotate dehydrogenase (EC 1.3.5.2) (DHOdehase) (DHOD) (DHODase) (Dihydroorotate oxidase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo450.146CYT0-0.200913-00="0"iCytoplasmicMembrane1012.87
DHPS_ECOLIP0AC13
folP

dhpS

dhpSb3177 JW3144 Dihydropteroate synthase (DHPS) (EC 2.5.1.15) (Dihydropteroate pyrophosphorylase)CytoplasmicAACytoplasmicN/AyesyesNo300.138CYT0-0.200913-00="0"iCytoplasmic9.9724.31
ILVD_ECOLIP05791
ilvD
b3771 JW5605 Dihydroxy-acid dehydratase (DAD) (EC 4.2.1.9)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AnoyesNo540.166CYT0-0.200913-00="0"iCytoplasmic9.9725.68
DTPA_ECOLIP77304
dtpA

tppB ydgR

tppB ydgRb1634 JW1626 Dipeptide and tripeptide permease AIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo190.114CYT0-0.200913-1414="0"i21-40o60-81i90-107o113-131i152-173o179-199i220-239o245-264i276-295o323-341i353-371o391-411i423-444o456-477iCytoplasmicMembrane10
DTPB_ECOLIP36837
dtpB

yhiP

yhiPb3496 JW3463 Dipeptide and tripeptide permease BIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo610.154TMH06.34727-1414="0"i28-46o52-72i84-101o107-124i145-167o173-193i214-232o238-256i268-285o320-337i349-371o383-408i415-437o457-479iCytoplasmicMembrane10
DTPD_ECOLIP75742
dtpD

ybgH

ybgHb0709 JW0699 Dipeptide permease DIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo480.16TMH01.28832-1414="0"i12-36o48-69i78-96o102-121i142-162o168-187i208-231o237-255i267-284o304-332i344-365o377-398i410-430o462-479iCytoplasmicMembrane10
DPPD_ECOLIP0AAG0
dppD
b3541 JW3510 Dipeptide transport ATP-binding protein dppDPeripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo660.286CYT0-0.200913-00="0"iCytoplasmicMembrane7.8819.13
DPPF_ECOLIP37313
dppF

dppE

dppEb3540 JW3509 Dipeptide transport ATP-binding protein dppFPeripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo530.133CYT0-0.200913-00="0"iCytoplasmicMembrane9.9924.02
DPPB_ECOLIP0AEF8
dppB
b3543 JW3512 Dipeptide transport system permease protein dppBIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo260.168TMH06.13051-66="0"i9-30o100-123i135-160o201-221i259-285o305-331iCytoplasmicMembrane10
DPPC_ECOLIP0AEG1
dppC
b3542 JW3511 Dipeptide transport system permease protein dppCIntegral inner membrane (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPYidCyesyesNo590.13TMH00.391748-55="0"o32-53i102-127o147-173i215-244o264-287iCytoplasmicMembrane104.14
DSBB_ECOLIP0A6M2
dsbB

roxB ycgA

roxB ycgAb1185 JW5182 Disulfide bond formation protein B (Disulfide oxidoreductase)Integral inner membrane (Experimental) Alpha-helcal transmembrane protein (Experimental) Integral inner membrane (Probable)Exhibites aberrant localization by microscopy in the absence of YidC PMID: 22108384. The crystal structure revealed a transmembrane (TM) four-helix-bundle structure of DsbBPMID: 18406599. DsbB oxidizes the periplasmic protein DsbA, which in turn oxidizes the cysteines in other periplasmic proteins to make disulfide bondsPMID: 8430071. Gene fusion approaches were used to show that (i) DsbB is a membrane protein which spans the membrane four times and (ii) both the N- and C-termini of the protein are in the cytoplasmPMID: 7957076. The structure of DsbB revealed four transmembrane helices and one short horizontal helix juxtaposed with Cys130 in the mobile periplasmic loopPMID: 17110337. Disulfide bridge (41 44;104 130) PMID: 10064586PMID: 18406599 PMID: 17110337 PMID: 8430071 PMID: 7957076 PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPYidCyesyesNo250.286TMH04.8983-44="0"i12-33o45-64i71-89o145-166iCytoplasmicMembrane1015.79
CUTA_ECOLIP69488
cutA

cutA1 cycY

cutA1 cycYb4137 JW4097 Divalent-cation tolerance protein cutA (C-type cytochrome biogenesis protein cycY)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo250.35CYT0-0.200913-00="0"iCytoplasmic9.26
DICB_ECOLIP09557
dicB
b1575 JW1566 Division inhibition protein dicBCytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo280.134CYT0-0.200913-00="0"iUnknown2
DMA_ECOLIP0AEE8
dam
b3387 JW3350 DNA adenine methylase (EC 2.1.1.72) (DNA adenine methyltransferase) (Deoxyadenosyl-methyltransferase) (M.EcoDam)Nucleoid (Experimental) Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication. 63007696300769NucleoidNNCytoplasmicN/AyesyesNo540.186CYT0-0.200913-00="0"iCytoplasmic8.96
GYRI_ECOLIP33012
sbmC

gyrI yeeB

gyrI yeeBb2009 JW1991 DNA gyrase inhibitory protein (Protein sbmC)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo690.101CYT0-0.200913-00="0"iCytoplasmic10132.56
GYRA_ECOLIP0AES4
gyrA

hisW nalA parD

hisW nalA parDb2231 JW2225 DNA gyrase subunit A (EC 5.99.1.3)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo360.106CYT0-0.200913-00="0"iCytoplasmic9.9749.36
GYRB_ECOLIP0AES6
gyrB

acrB himB hisU nalC parA pcbA

acrB himB hisU nalC parA pcbAb3699 JW5625 DNA gyrase subunit B (EC 5.99.1.3)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo540.109CYT0-0.200913-00="0"iCytoplasmic9.97
UVRD_ECOLIP03018
uvrD

mutU pdeB rad recL

mutU pdeB rad recLb3813 JW3786 DNA helicase II (EC 3.6.4.12)Nucleoid (Experimental) Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair. 84192858419285NucleoidNNCytoplasmicN/AyesyesNo200.137CYT0-0.200913-00="0"iCytoplasmic9.9720.35
DNLJ_ECOLIP15042
ligA

dnaL lig lop pdeC

dnaL lig lop pdeCb2411 JW2403 DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+])CytoplasmicAACytoplasmicN/AyesyesNo650.109CYT0-0.200913-00="0"iCytoplasmic9.9759.05
LIGB_ECOLIP25772
ligB

yicF

yicFb3647 JW3622 DNA ligase B (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+] B)CytoplasmicAACytoplasmicN/AyesyesYes210.685SpI303.29037-00Yn3-14c20/21oCytoplasmic9.2612.07
MUTH_ECOLIP06722
mutH

mutR prv

mutR prvb2831 JW2799 DNA mismatch repair protein mutH (Methyl-directed mismatch repair protein)Nucleoid (Experimental) Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair. 94827499482749NucleoidNNCytoplasmCytoplasmicN/AyesyesNo340.124CYT0-0.200913-00="0"iCytoplasmic10100.33
MUTL_ECOLIP23367
mutL
b4170 JW4128 DNA mismatch repair protein mutLNucleoid (Experimental) This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. PMID: 1019940510199405NucleoidNNCytoplasmicN/AyesyesNo400.132CYT0-0.200913-00="0"iCytoplasmic9.97
MUTS_ECOLIP23909
mutS

fdv

fdvb2733 JW2703 DNA mismatch repair protein mutSNucleoid (Experimental) This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity. 19389371938937NucleoidNNCytoplasmicIM (predicted)yesyesNo630.202CYT0-0.200913-00="0"iCytoplasmic9.9742.11
DPO1_ECOLIP00582
polA

resA

resAb3863 JW3835 DNA polymerase I (POL I) (EC 2.7.7.7)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AnoyesNo590.268CYT0-0.200913-00="0"iCytoplasmic9.9776.46
DPO2_ECOLIP21189
polB

dinA

dinAb0060 JW0059 DNA polymerase II (Pol II) (EC 2.7.7.7)CytoplasmicAACytoplasmicN/AnoyesNo370.111CYT0-0.200913-00="0"iCytoplasmic9.97
DPO3A_ECOLIP10443
dnaE

polC

polCb0184 JW0179 DNA polymerase III subunit alpha (EC 2.7.7.7)NucleoidNNCytoplasmCytoplasmicN/AyesyesNo410.148CYT0-0.200913-00="0"iCytoplasmic1032.1
DPO3B_ECOLIP0A988
dnaN
b3701 JW3678 DNA polymerase III subunit beta (EC 2.7.7.7)Cytoplasm (Probable)PMID: 23994470NucleoidNNCytoplasmCytoplasmicN/AyesyesNo240.11CYT0-0.200913-00="0"iCytoplasmic1016.07
HOLC_ECOLIP28905
holC
b4259 JW4216 DNA polymerase III subunit chi (EC 2.7.7.7)NucleoidNNCytoplasmicN/AyesyesNo640.107CYT0-0.200913-00="0"iCytoplasmic9.2670.24
HOLA_ECOLIP28630
holA
b0640 JW0635 DNA polymerase III subunit delta (EC 2.7.7.7)NucleoidNNCytoplasmicN/AyesyesNo120.108CYT0-0.200913-00="0"iCytoplasmicMembrane9.8234.75
HOLB_ECOLIP28631
holB
b1099 JW1085 DNA polymerase III subunit delta' (EC 2.7.7.7)NucleoidNNCytoplasmicN/AyesyesNo320.127CYT0-0.200913-00="0"iCytoplasmic9.2637.8
DPO3E_ECOLIP03007
dnaQ

mutD

mutDb0215 JW0205 DNA polymerase III subunit epsilon (EC 2.7.7.7)NucleoidNNCytoplasmicN/AyesyesNo240.113CYT0-0.200913-00="0"iCytoplasmic9.9713.32
HOLD_ECOLIP28632
holD
b4372 JW4334 DNA polymerase III subunit psi (EC 2.7.7.7)NucleoidNNCytoplasmicN/AnoyesNo510.127CYT0-0.200913-00="0"iUnknown231.67
DPO3X_ECOLIP06710
dnaX

dnaZ dnaZX

dnaZ dnaZXb0470 JW0459 DNA polymerase III subunit tau (EC 2.7.7.7) [Cleaved into: DNA polymerase III subunit gamma]NucleoidNNCytoplasmicN/AyesyesNo170.18CYT0-0.200913-00="0"iCytoplasmic9.97
HOLE_ECOLIP0ABS8
holE
b1842 JW1831 DNA polymerase III subunit theta (EC 2.7.7.7)NucleoidNNCytoplasmicN/AnoyesNo380.197CYT0-0.200913-00="0"iCytoplasmic1096.24
DPO4_ECOLIQ47155
dinB

dinP

dinPb0231 JW0221 DNA polymerase IV (Pol IV) (EC 2.7.7.7)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo260.127CYT0-0.200913-00="0"iCytoplasmic9.9725.65
DNAG_ECOLIP0ABS5
dnaG

dnaP parB

dnaP parBb3066 JW3038 DNA primase (EC 2.7.7.-)NucleoidNNCytoplasmicIM (predicted)yesyesNo520.101CYT0-0.200913-00="0"iCytoplasmic9.9739.84
DPS_ECOLIP0ABT2
dps

pexB vtm

pexB vtmb0812 JW0797 DNA protection during starvation protein (EC 1.16.-.-)Nucleoid-associated protein (NAP) (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)Nucleoid-associated protein (NAP) Dodecameric Dps forms a protein cage with a hollow interior that sequesters Fe 2+ and reduces hydroxyl radical formation PMID: 23701194 PMID: 7984106PMID: 21541338 PMID: 23230279 PMID: 7984106Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1Cytoplasm nucleoidCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo530.125CYT0-0.200913-00="0"iCytoplasmic1044.48
RMUC_ECOLIP0AG71
rmuC

yigN

yigNb3832 JW3809 DNA recombination protein rmuCSingle-pass integral inner membrane protein (Potential)Based on the fact that RmuC is a DNA recombination enzyme we conluded that its N-terminus lays in the periplasm.PMID: 16011798Integral Inner Membrane BBout/inCytoplasmicN/ASRPSECyesyesNo290.536SpI281.08238-11="0"o6-25iUnknown215.99
RADA_ECOLIP24554
radA

sms

smsb4389 JW4352 DNA repair protein radA (DNA repair protein sms)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo410.199CYT0-0.200913-00="0"iCytoplasmicMembrane9.8229.48
RECN_ECOLIP05824
recN

radB

radBb2616 JW5416 DNA repair protein recN (Recombination protein N)Cytoplasm (Probable)PMID: 21219465CytoplasmicAACytoplasmicIM (predicted)yesyesNo530.178CYT0-0.200913-00="0"iCytoplasmic9.9712.67
RECO_ECOLIP0A7H3
recO
b2565 JW2549 DNA repair protein recO (Recombination protein O)CytoplasmicAACytoplasmicIM (predicted)yesyesNo220.123CYT0-0.200913-00="0"iCytoplasmic9.26
RECF_ECOLIP0A7H0
recF

uvrF

uvrFb3700 JW3677 DNA replication and repair protein recFPeripheral inner membrane protein facing the cytoplasm (Probable) Nucleoid (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. The prokaryotic protein recF is a single-stranded DNA-binding protein which also probably binds ATP. RecF is involved in DNA metabolism; it is required for recombinational DNA repair and for induction of the SOS response. PMID: 1542576PMID: 19766000 PMID: 1542576Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo320.128CYT0-0.200913-00="0"iCytoplasmic9.976.3
DNAC_ECOLIP0AEF0
dnaC

dnaD

dnaDb4361 JW4325 DNA replication protein dnaCCytoplasmicAACytoplasmicN/AyesyesNo200.111CYT0-0.200913-00="0"iCytoplasmic8.96
TUS_ECOLIP16525
tus

tau

taub1610 JW1602 DNA replication terminus site-binding protein (Ter-binding protein)Nucleoid (Probable)Binds to Ter-chromosomal site.PMID: 16148308NucleoidNNCytoplasmCytoplasmicN/AyesyesNo260.107CYT0-0.200913-00="0"iCytoplasmic104.71
TOP1_ECOLIP06612
topA

supX

supXb1274 JW1266 DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I) (Omega-protein) (Relaxing enzyme) (Swivelase) (Untwisting enzyme)Copurifies with trigger factor in pull down assays.NucleoidNNCytoplasmicN/ATFyesyesNo150.14CYT0-0.200913-00="0"iCytoplasmic9.97
TOP3_ECOLIP14294
topB
b1763 JW1752 DNA topoisomerase 3 (EC 5.99.1.2) (DNA topoisomerase III)NucleoidNNCytoplasmicN/AyesyesNo500.13CYT0-0.200913-00="0"iCytoplasmic9.97
PARC_ECOLIP0AFI2
parC
b3019 JW2987 DNA topoisomerase 4 subunit A (EC 5.99.1.-) (Topoisomerase IV subunit A)Peripheral inner membrane protein facing the cytoplasm (Experimental)Dimer of topoisomerase IV subunit A (bioCyc) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1334483 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1Cell membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo180.118CYT0-0.200913-00="0"iCytoplasmicMembrane1017.79
PARE_ECOLIP20083
parE

nfxD

nfxDb3030 JW2998 DNA topoisomerase 4 subunit B (EC 5.99.1.-) (Topoisomerase IV subunit B)NucleoidNNCytoplasmicN/AyesyesNo100.105CYT0-0.200913-00="0"iCytoplasmic1077.34
FTSK_ECOLIP46889
ftsK
b0890 JW0873 DNA translocase ftsKCell inner membrane (Probable) Integral inner membrane (Probable)PMID: 9723913 PMID: 11703663 PMID: 11948172 PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane protein | Cytoplasmic sideIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo530.169TMH06.50875-55="0"i25-44o75-98i110-131o137-158i165-184oCytoplasmicMembrane1014.84
3MG1_ECOLIP05100
tag
b3549 JW3518 DNA-3-methyladenine glycosylase 1 (EC 3.2.2.20) (3-methyladenine-DNA glycosylase I, constitutive) (TAG I) (DNA-3-methyladenine glycosidase I) (DNA-3-methyladenine glycosylase I)CytoplasmicAACytoplasmicN/AnoyesNo230.11CYT0-0.200913-00="0"iUnknown263.2
3MG2_ECOLIP04395
alkA

aidA

aidAb2068 JW2053 DNA-3-methyladenine glycosylase 2 (EC 3.2.2.21) (3-methyladenine-DNA glycosylase II, inducible) (TAG II) (DNA-3-methyladenine glycosidase II) (DNA-3-methyladenine glycosylase II)CytoplasmicAACytoplasmicN/AyesyesNo320.186CYT0-0.200913-00="0"iUnknown220.43
FIS_ECOLIP0A6R3
fis
b3261 JW3229 DNA-binding protein fis (Factor-for-inversion stimulation protein) (Hin recombinational enhancer-binding protein)Nucleoid-associated protein (NAP) (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoid-associated protein (NAP).PMID: 21541338 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AyesyesNo190.116CYT0-0.200913-00="0"iCytoplasmic9.26110.24
HNS_ECOLIP0ACF8
hns

bglY cur drdX hnsA msyA osmZ pilG topS

bglY cur drdX hnsA msyA osmZ pilG topSb1237 JW1225 DNA-binding protein H-NS (Histone-like protein HLP-II) (Protein B1) (Protein H1)Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338.PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AyesyesNo190.173CYT0-0.200913-00="0"iCytoplasmic10103.34
DBHA_ECOLIP0ACF0
hupA
b4000 JW3964 DNA-binding protein HU-alpha (HU-2) (NS2)Nucleoid-associated protein (NAP) (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Nucleoid (Potential)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338. Bacterial histone-like DNA-binding proteins signature (PDOC00044).PMID: 21541338 PMID: 23230279 Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AyesyesNo240.244CYT0-0.200913-00="0"iCytoplasmic9.2696.48
DBHB_ECOLIP0ACF4
hupB

hopD

hopDb0440 JW0430 DNA-binding protein HU-beta (HU-1) (NS1)Nucleoid (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental) Nucleoid (Potential)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338. Bacterial histone-like DNA-binding proteins signature PMID: 21541338 PMID: 23230279 Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AyesyesNo270.114CYT0-0.200913-00="0"iCytoplasmic9.26
STPA_ECOLIP0ACG1
stpA

hnsB

hnsBb2669 JW2644 DNA-binding protein stpA (H-NS homolog stpA)Nucleoid-associated protein (NAP) (Experimental) Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338.PMID: 21541338 PMID: 23230279Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmicN/AyesyesNo190.184CYT0-0.200913-00="0"iCytoplasmic9.2615.73
DCM_ECOLIP0AED9
dcm

mec

mecb1961 JW1944 DNA-cytosine methyltransferase (EC 2.1.1.37) (M.EcoDcm)CytoplasmicAACytoplasmicN/AnoyesNo480.119CYT0-0.200913-00="0"iCytoplasmic8.9642.86
DIND_ECOLIP23840
dinD

pcsA yicD

pcsA yicDb3645 JW3620 DNA-damage-inducible protein DCytoplasmicAACytoplasmicN/AnoyesNo400.11CYT0-0.200913-00="0"iCytoplasmic8.96
DINF_ECOLIP28303
dinF
b4044 JW4004 DNA-damage-inducible protein FCell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo210.258TMH03.41253-1313="0"o6-22i29-49o61-83i103-124o152-173i180-199o205-227i259-278o284-302i338-359o371-393i405-422o428-446iCytoplasmicMembrane1010.07
DINI_ECOLIP0ABR1
dinI
b1061 JW1048 DNA-damage-inducible protein ICytoplasmicAACytoplasmicN/AyesyesNo230.175CYT0-0.200913-00="0"iUnknown2
DINJ_ECOLIQ47150
dinJ
b0226 JW0216 DNA-damage-inducible protein JNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AnoyesNo200.147CYT0-0.200913-00="0"iUnknown283.04
RPOA_ECOLIP0A7Z4
rpoA

pez phs sez

pez phs sezb3295 JW3257 DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) (EC 2.7.7.6) (RNA polymerase subunit alpha) (Transcriptase subunit alpha)Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23230279 PMID: 21541338Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo250.163CYT0-0.200913-00="0"iCytoplasmic9.97
RPOB_ECOLIP0A8V2
rpoB

groN nitB rif ron stl stv tabD

groN nitB rif ron stl stv tabDb3987 JW3950 DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo400.117CYT0-0.200913-00="0"iCytoplasmic9.97
RPOC_ECOLIP0A8T7
rpoC

tabB

tabBb3988 JW3951 DNA-directed RNA polymerase subunit beta' (RNAP subunit beta') (EC 2.7.7.6) (RNA polymerase subunit beta') (Transcriptase subunit beta')Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo260.118CYT0-0.200913-00="0"iCytoplasmic9.97
RPOZ_ECOLIP0A800
rpoZ
b3649 JW3624 DNA-directed RNA polymerase subunit omega (RNAP omega subunit) (EC 2.7.7.6) (RNA polymerase omega subunit) (Transcriptase subunit omega)Peripheral inner membrane protein facing the cytoplasm (Experimental)The presence of omega subunit of RNA polymerase (RpoZ) is necessary for maturation of RNA polymerase. Experimentally verified gene-product. Methionine start codon is cleaved.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo350.113CYT0-0.200913-00="0"iCytoplasmic8.9681.6
PINE_ECOLIP03014
pinE

pin

pinb1158 JW1144 DNA-invertase from lambdoid prophage e14CytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo630.111CYT0-0.200913-00="0"iCytoplasmic8.9616.83
DIAA_ECOLIP66817
diaA

yraO

yraOb3149 JW3118 DnaA initiator-associating protein diaACytoplasmicAACytoplasmicN/AyesyesNo570.143CYT0-0.200913-00="0"iCytoplasmic9.9770.18
HDA_ECOLIP69931
hda

idaB yfgE

idaB yfgEb2496 JW5397 DnaA-homolog protein hdaPeripheral inner membrane protein facing the cytoplasm (Probable)Homolgous to DnaA and more specific to C-terminal anchor of DnaA.PMID: 12618445Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo330.157CYT0-0.200913-00="0"iCytoplasmic9.97
DJLA_ECOLIP31680
djlA

yabH

yabHb0055 JW0054 DnaJ-like protein djlACell inner membrane (Probable)PMID: 11106641Integral Inner Membrane BBout/inCell inner membrane | Single-pass type III membrane proteinMembrane anchoredIM (predicted), Detected in IM FractionSRPSECyesyesNo220.135TMH010.4559-11="0"o6-30iCytoplasmicMembrane1011.88
DKSA_ECOLIP0ABS1
dksA
b0145 JW0141 DnaK suppressor proteinCytoplasm (Probable) Nucleoid (Experimental) Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters. PMID: 15294157PMID: 16858726 PMID: 15294157NucleoidNNCytoplasmicN/AyesyesNo260.123CYT0-0.200913-00="0"iCytoplasmic9.9772.85
DSDX_ECOLIP08555
dsdX

yfdA yfdD

yfdA yfdDb2365 JW2362 DsdX permeaseIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein (Experimental)IM (predicted)SRPSECnoyesNo330.118TMH011.5143-1212="0"o6-23i30-47o59-77i98-128o140-158i178-198o225-244i256-282o302-322i343-365o385-408i420-444oCytoplasmicMembrane1012.2
RMLC_ECOLIP37745
rfbC

rmlC

rmlCb2038 JW2023 dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13) (Thymidine diphospho-4-keto-rhamnose 3,5-epimerase) (dTDP-4-keto-6-deoxyglucose 3,5-epimerase) (dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase) (dTDP-L-rhamnose synthase)CytoplasmicAACytoplasmicN/AnoyesNo210.127CYT0-0.200913-00="0"iCytoplasmic8.96
RMLD_ECOLIP37760
rfbD

rmlD

rmlDb2040 JW2025 dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) (dTDP-4-keto-L-rhamnose reductase) (dTDP-6-deoxy-L-lyxo-4-hexulose reductase) (dTDP-6-deoxy-L-mannose dehydrogenase) (dTDP-L-rhamnose synthase)CytoplasmicAACytoplasmicN/AnoyesNo340.18CYT0-0.200913-00="0"iCytoplasmic9.26
RMLB1_ECOLIP37759
rfbB

rmlB

rmlBb2041 JW2026 dTDP-glucose 4,6-dehydratase 1 (EC 4.2.1.46)Trigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo210.115CYT0-0.200913-00Yn3-13c18/19oCytoplasmic9.9723.37
RMLB2_ECOLIP27830
rffG
b3788 JW5598 dTDP-glucose 4,6-dehydratase 2 (EC 4.2.1.46)CytoplasmicAACytoplasmicN/AyesyesNo510.153CYT0-0.200913-00="0"iCytoplasmic9.9740.62
YFBO_ECOLIP76485
yfbO
b2274 JW2269 dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase (EC 2.4.-.-) (Rhamnosyltransferase)CytoplasmicAACytoplasmicN/AnoyesNo410.116CYT0-0.200913-00="0"iUnknown284.87
YFIP_ECOLIQ47319
yfiP
b2583 JW5409 DTW domain-containing protein yfiPCytoplasmicAACytoplasmicN/AyesyesNo520.302CYT0-0.200913-00="0"iCytoplasmic9.9737.14
ECOT_ECOLIP23827
eco

eti

etib2209 JW2197 EcotinPeriplasmic (Probable) Periplasmic (Experimental) Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544PMID: 15911532 PMID: 9298646PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes210.889SpI2016.5012-00Yn4-15c20/21oPeriplasmic1085.11
RNFA_ECOLIP0A766
rnfA

ydgL

ydgLb1627 JW1619 Electron transport complex protein rnfAIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinIntegral Membrane Protein(experimental)IM (predicted)SRPSECyesyesNo210.118TMH07.31415-66="0"o6-26i38-59o65-87i99-122o134-155i167-191oCytoplasmicMembrane10
RNFB_ECOLIP77223
rnfB

ydgM

ydgMb1628 JW1620 Electron transport complex protein rnfBPeripheral inner membrane protein facing the cytoplasm (Experimental)Electron transport. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell inner membrane | Peripheral membrane proteinPeriplasmicN/AyesyesNo310.278SpI304.42273-10Yn3-14c19/20oCytoplasmicMembrane1022.68
RNFC_ECOLIP77611
rnfC

ydgN

ydgNb1629 JW1621 Electron transport complex protein rnfCPeripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo540.101CYT0-0.200913-00="0"iCytoplasmic8.96
RNFD_ECOLIP76182
rnfD

ydgO

ydgOb1630 JW1622 Electron transport complex protein rnfDIntegral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo340.191TMH05.44392-1010="0"i20-38o44-62i69-89o95-113i125-143o210-235i242-262o268-287i299-318o324-342iCytoplasmicMembrane10
RNFE_ECOLIP77179
rnfE

ydgQ

ydgQb1632 JW1624 Electron transport complex protein rnfEIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo380.173CYT0-0.200913-56="0"i12-32o38-57i69-87o93-113i125-148o183-206iCytoplasmicMembrane103.71
RNFG_ECOLIP77285
rnfG

ydgP

ydgPb1631 JW1623 Electron transport complex protein rnfG Disulfide bond-containing protein PMID: 25664544PeriplasmicGGCell inner membranePeriplasmicN/ASECyesyesYes250.427SpI2411.9411-00Yn9-17c24/25oUnknown2.542.9
HYDN_ECOLIP0AAK4
hydN
b2713 JW2683 Electron transport protein hydNCytoplasmicAACytoplasmicN/AyesyesNo220.125CYT0-0.200913-00="0"iCytoplasmic9.97102.19
EFG_ECOLIP0A6M8
fusA

far fus

far fusb3340 JW3302 Elongation factor G (EF-G)Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic (Experimental)Translation PMID: 7042386PMID: 23230279 PMID: 7042386Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmic (Experimental)N/AyesyesNo390.11CYT0-0.200913-00="0"iCytoplasmic9.9762.49
EFP_ECOLIP0A6N4
efp
b4147 JW4107 Elongation factor P (EF-P)TranslationCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo270.163CYT0-0.200913-00="0"iCytoplasmic10109.13
EFPL_ECOLIP0A6N8
yeiP
b2171 JW5362 Elongation factor P-like proteinCytoplasmicAACytoplasmicN/AnoyesNo350.164CYT0-0.200913-00="0"iCytoplasmic9.9791.8
EFTS_ECOLIP0A6P1
tsf
b0170 JW0165 Elongation factor Ts (EF-Ts)Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AnoyesNo590.109CYT0-0.200913-00="0"iCytoplasmic1087.2
EFTU1_ECOLIP0CE47
tufA
b3339 JW3301 Elongation factor Tu 1 (EF-Tu 1) (P-43)Peripheral inner membrane protein facing the cytoplasm (By similarity) Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic (Experimental) (Experimental)Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. PMID: 7525272 PMID: 7021545 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451 Disulfide bond-containing protein PMID: 25664544PMID: 20133608 PMID: 2022614 PMID: 775340 PMID: 7525272 PMID: 7021545 PMID: 20153451Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell membrane | Peripheral membrane proteinCytoplasmic (Experimental)N/AyesyesNo470.197CYT0-0.200913-00="0"iCytoplasmic1099.39
EFTU2_ECOLIP0CE48
tufB
b3980 JW3943 Elongation factor Tu 2 (EF-Tu 2) (P-43)Peripheral inner membrane protein facing the cytoplasm (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental) (Experimental)PMID: 2022614 PMID: 775340 PMID: 23230279 PMID: 20153451Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell membrane | Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo470.197CYT0-0.200913-00="0"iCytoplasmic1087.23
EMTA_ECOLIP0C960
emtA

mltE sltZ ycgP

mltE sltZ ycgPb1193 JW5821 Endo-type membrane-bound lytic murein transglycosylase A (EC 4.2.2.n2) (Peptidoglycan lytic endotransglycosylase)Lipoprotein (Experimental) Proven pignal peptidase II substrate. PMID: 15174130PMID: 15174130Outer Membrane LipoproteinIICell outer membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)N/ASECLOL: Localization Of LipoproteinsyesyesYes490.204SpII1518.8581Pos+2=S00Yn5-13c18/19oCytoplasmicMembrane9.8215.95
GUN_ECOLIP37651
bcsZ

bcsC yhjM

bcsC yhjMb3531 JW3499 Endoglucanase (EC 3.2.1.4) (Carboxymethylcellulase) (CMCase) (Cellulase) (Endo-1,4-beta-glucanase)Periplasmic (Experimental) (Experimental)PMID: 11260463 PMID: 21454578 PMID: 24097954PeriplasmicGGCell outer membranePeriplasmic (Experimental)N/ASECnoyesYes220.88SpII2212.5942Pos+2=T00Yn6-17c21/22oExtracellular106.23
END4_ECOLIP0A6C1
nfo
b2159 JW2146 Endonuclease 4 (EC 3.1.21.2) (Endodeoxyribonuclease IV) (Endonuclease IV)CytoplasmicAACytoplasmicN/AyesyesNo180.279CYT0-0.200913-00Yn4-15c21/22oCytoplasmic8.9613.85
END8_ECOLIP50465
nei
b0714 JW0704 Endonuclease 8 (DNA glycosylase/AP lyase Nei) (EC 3.2.2.-) (EC 4.2.99.18) (DNA-(apurinic or apyrimidinic site) lyase Nei) (Endonuclease VIII)Nucleoid (Experimental) Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. 20031487 Zinc ion binding protein 20031487NucleoidNNCytoplasmicN/AnoyesNo700.114CYT0-0.200913-00="0"iCytoplasmic9.9793.46
END3_ECOLIP0AB83
nth
b1633 JW1625 Endonuclease III (EC 4.2.99.18) (DNA-(apurinic or apyrimidinic site) lyase)Nucleoid (Experimental) DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. 2449657PMID: 2449657NucleoidNNCytoplasmicN/AyesyesNo430.298CYT0-0.200913-00="0"iCytoplasmic9.9757.47
NFI_ECOLIP68739
nfi

yjaF

yjaFb3998 JW5547 Endonuclease V (EC 3.1.21.7) (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V) (DNase V)Nucleoid (Experimental) DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. 82069318206931NucleoidNNCytoplasmCytoplasmicN/AyesyesNo200.108CYT0-0.200913-00="0"iCytoplasmic1022.54
END1_ECOLIP25736
endA
b2945 JW2912 Endonuclease-1 (EC 3.1.21.1) (Endonuclease I) (Endo I)Periplasm (Probable) Periplasmic (Experimental) Identified DsbA substrate PMID: 19953543 PMID: 7867949 Disulfide bond-containing protein PMID: 25664544PMID: 4955462 PMID: 7867949PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECDsbAnoyesYes230.883SpI2222.5344-00Yn5-16c22/23oUnknown5.0299.73
ELBB_ECOLIP0ABU5
elbB

elb2 yzzB

elb2 yzzBb3209 JW3176 Enhancing lycopene biosynthesis protein 2 (Sigma cross-reacting protein 27A) (SCRP-27A)CytoplasmicAACytoplasmicN/AyesyesNo370.458CYT0-0.200913-00="0"iCytoplasmic9.9785.86
ENO_ECOLIP0A6P9
eno
b2779 JW2750 Enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic (Experimental) Excreted (Probable) Cytoskeleton (Probable) Peripheral inner membrane protein facing the cytoplasm (Experimental)When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. PMID: 8610017 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 17242352 PMID: 8610017 PMID: 15003462 PMID: 18337249 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceA, F1, F4A,F1,F4Cytoplasm -> cytoskeleton | Secreted | Cell surfaceCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionUknown mechanismyesyesNo420.139CYT0-0.200913-00="0"iCytoplasmic9.97100.97
FABI_ECOLIP0AEK4
fabI

envM

envMb1288 JW1281 Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (NADH-dependent enoyl-ACP reductase)Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/ATFyesyesNo280.245CYT0-0.200913-00="0"iCytoplasmicMembrane1048.83
PAAD_ECOLIP76080
paaD

ydbQ

ydbQb1391 JW5217 Entericidin ACytoplasmicAACytoplasmicN/AnoyesNo460.106CYT0-0.200913-00="0"iUnknown2116.69
ECNB_ECOLIP0ADB7
ecnB

yjeU

yjeUb4411 JW4108 Entericidin BOuter membrane Lipoprotein (Experimental) Outer membrane protein (Experimental)Potentially toxic protein. Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIICell membrane | Lipid-anchorOuter membrane Lipoprotein (Experimental)Soluble protein detected in Inner Membrane FractionSECLOL: Localization Of LipoproteinsnoyesYes220.27SpII2117.5085Pos+2=N10Yn5-15c20/21oCytoplasmicMembrane10
ENTS_ECOLIP24077
entS

ybdA

ybdAb0591 JW0583 Enterobactin exporter entS (Protein p43)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo210.337TMH010.2914-1112="0"i21-42o54-74i86-105o111-135i147-170o176-199i219-246o258-276i288-305o311-336i357-374o380-399iCytoplasmicMembrane10
ENTE_ECOLIP10378
entE
b0594 JW0586 Enterobactin synthase component E (Enterochelin synthase E) [Includes: 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) (Dihydroxybenzoic acid-activating enzyme); S-dihydroxybenzoyltransferase (EC 2.3.1.-)]Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous to FadD13 protein of Mycobacterium that is peripheral. Phobius predits transmembrane region around aa100. Binds AMP. Very high homology to eight other transport proteins: (FADK_ECOLI, CAIC_ECOLI, LCFA_ECOLI, ENTF_ECOLI, ACSA_ECOLI) Membrane Association of the Escherichia coli Enterobactin Synthase Proteins EntB/G, EntE, and EntFPMID: 10692387Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo360.15CYT0-0.200913-01="0"o86-105iCytoplasmic9.9788.29
ENTF_ECOLIP11454
entF
b0586 JW0578 Enterobactin synthase component F (EC 2.7.7.-) (Enterochelin synthase F) (Serine-activating enzyme) (Seryl-AMP ligase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 10692387Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo560.235CYT0-0.200913-00="0"iCytoplasmic9.9720.96
FES_ECOLIP13039
fes
b0585 JW0576 Enterochelin esterase (Ferric enterobactin esterase)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo600.115CYT0-0.200913-00="0"iCytoplasmic109.94
YFCH_ECOLIP77775
yfcH
b2304 JW2301 Epimerase family protein yfcHCytoplasmicAACytoplasmicN/AyesyesNo530.107CYT0-0.200913-00="0"iCytoplasmic9.9716.55
PDXB_ECOLIP05459
pdxB
b2320 JW2317 Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290)CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo680.143CYT0-0.200913-00="0"iCytoplasmic9.9715.26
FRSA_ECOLIP04335
frsA

yafA

yafAb0239 JW0229 Esterase frsA (EC 3.1.-.-)CytoplasmicAACytoplasmicN/AyesyesNo380.126CYT0-0.200913-00="0"iUnknown213.08
ENTH_ECOLIP0A8Y8
entH

ybdB

ybdBb0597 JW0589 Esterase ybdB (EC 3.1.-.-) (p15)Cytoplasm (Probable)PMID: 17675380CytoplasmicAACytoplasmicN/AyesyesNo700.132CYT0-0.200913-00="0"iUnknown280.45
YBFF_ECOLIP75736
ybfF
b0686 JW0673 Esterase ybfF (EC 3.1.-.-)CytoplasmicAACytoplasmicN/AyesyesNo370.368CYT0-0.200913-00="0"iUnknown232.96
YDII_ECOLIP77781
ydiI
b1686 JW1676 Esterase ydiI (EC 3.1.-.-)CytoplasmicAACytoplasmicN/AyesyesNo630.118CYT0-0.200913-00="0"iCytoplasmic8.9678.47
YJFP_ECOLIP39298
yjfP
b4190 JW4148 Esterase yjfP (EC 3.1.-.-)CytoplasmicAACytoplasmicN/AyesyesNo540.138CYT0-0.200913-00="0"iUnknown240.03
YPFH_ECOLIP76561
ypfH
b2473 JW5396 Esterase ypfH (EC 3.1.-.-)CytoplasmicAACytoplasmicN/AyesyesNo340.18CYT0-0.200913-00="0"iUnknown2103.17
YQIA_ECOLIP0A8Z7
yqiA

yzzI

yzzIb3031 JW2999 Esterase yqiA (EC 3.1.-.-)CytoplasmicAACytoplasmicN/AyesyesNo200.301CYT0-0.200913-00="0"iCytoplasmic8.9617.19
EUTB_ECOLIP0AEJ6
eutB
b2441 JW2434 Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7) (Ethanolamine ammonia-lyase large subunit)CytoplasmicAACytoplasmicN/AnoyesNo350.21CYT0-0.200913-00="0"iUnknown4.9913.34
EUTC_ECOLIP19636
eutC
b2440 JW2433 Ethanolamine ammonia-lyase light chain (EC 4.3.1.7) (Ethanolamine ammonia-lyase small subunit)CytoplasmicAACytoplasmicN/AnoyesNo280.196CYT0-0.200913-00="0"iCytoplasmic9.9717.04
EUTT_ECOLIP65643
eutT

ypfB

ypfBb2459 JW2443 Ethanolamine utilization cobalamin adenosyltransferase (EC 2.5.1.17)CytoplasmicAACytoplasmicN/AyesyesNo210.108CYT0-0.200913-00="0"iCytoplasmic8.96106.42
EUTA_ECOLIP76551
eutA

yffT

yffTb2451 JW2435 Ethanolamine utilization protein eutACytoplasmicAACytoplasmicIM (predicted)noyesNo350.117CYT0-0.200913-00="0"iCytoplasmicMembrane9.8233.47
EUTD_ECOLIP77218
eutD

eutI ypfA

eutI ypfAb2458 JW2442 Ethanolamine utilization protein eutDCytoplasmicAACytoplasmicN/AyesyesNo310.15CYT0-0.200913-00="0"iCytoplasmic9.9782.48
EUTE_ECOLIP77445
eutE

yffX

yffXb2455 JW2439 Ethanolamine utilization protein eutECytoplasmicAACytoplasmicN/AyesyesNo260.189CYT0-0.200913-00="0"iCytoplasmic8.9675.7
EUTG_ECOLIP76553
eutG

yffV

yffVb2453 JW2437 Ethanolamine utilization protein eutG (EC 1.1.-.-) Alcohol dehydrogenase, iron-type, conserved site (IPR018211) CytoplasmicAACytoplasmicN/AyesyesNo640.179CYT0-0.200913-00="0"iCytoplasmicMembrane8.469.26
EUTH_ECOLIP76552
eutH

yffU

yffUb2452 JW2436 Ethanolamine utilization protein eutH (Putative ethanolamine transporter)Cell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo700.135TMH05.16844-109="0"o6-24i57-84o120-146i153-178o190-215i227-250o270-294i342-364o370-392iCytoplasmicMembrane10
EUTJ_ECOLIP77277
eutJ

yffW

yffWb2454 JW2438 Ethanolamine utilization protein eutJCytoplasmicAACytoplasmicN/AyesyesNo250.126CYT0-0.200913-00="0"iCytoplasmic8.9681.21
EUTK_ECOLIP76540
eutK

yffI

yffIb2438 JW2431 Ethanolamine utilization protein eutKCytoplasmicAACytoplasmicN/AyesyesNo200.135CYT0-0.200913-00="0"iUnknown279.97
EUTL_ECOLIP76541
eutL

yffJ

yffJb2439 JW2432 Ethanolamine utilization protein eutLCytoplasmicAACytoplasmicN/AyesyesNo260.167CYT0-0.200913-00="0"iUnknown220.15
EUTM_ECOLIP0ABF4
eutM

cchA yffZ

cchA yffZb2457 JW2441 Ethanolamine utilization protein eutMCytoplasmicAACytoplasmicN/AyesyesNo510.135CYT0-0.200913-00="0"iUnknown288.93
EUTN_ECOLIP0AEJ8
eutN

cchB yffY

cchB yffYb2456 JW2440 Ethanolamine utilization protein eutNCytoplasmicAACytoplasmicN/AyesyesNo210.137CYT0-0.200913-00="0"iUnknown257.16
EUTP_ECOLIP76556
eutP

ypfD

ypfDb2461 JW2445 Ethanolamine utilization protein eutPCytoplasmicAACytoplasmicN/AyesyesNo210.132CYT0-0.200913-00="0"iUnknown290.45
EUTQ_ECOLIP76555
eutQ

ypfC

ypfCb2460 JW2444 Ethanolamine utilization protein eutQCytoplasmicAACytoplasmicN/AyesyesNo570.129CYT0-0.200913-00="0"iCytoplasmic8.9688.59
EUTS_ECOLIP63746
eutS

ypfE

ypfEb2462 JW2446 Ethanolamine utilization protein eutSCytoplasmicAACytoplasmicN/AyesyesNo310.162CYT0-0.200913-00="0"iCytoplasmic8.9692.74
BGA2_ECOLIP06864
ebgA
b3076 JW5511 Evolved beta-galactosidase subunit alpha (Beta-gal) (EC 3.2.1.23) (Lactase)CytoplasmicAACytoplasmicN/AyesyesNo310.217CYT0-0.200913-00="0"iUnknown221.62
EBGC_ECOLIP0AC73
ebgC
b3077 JW3048 Evolved beta-galactosidase subunit betaCytoplasmicAACytoplasmicN/AyesyesNo410.111CYT0-0.200913-00="0"iCytoplasmic8.9623.52
CHO_ECOLIP76213
cho

ydjQ

ydjQb1741 JW1730 Excinuclease cho (EC 3.1.25.-) (Endonuclease cho) (UvrC homolog protein)Nucleoid (Experimental) Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than UvrC. Not able to incise the 5' site of a lesion. In vitro, the incision activity of Cho is UvrA and UvrB dependent. When a lesion remains because UvrC is not able to induce the 3' incision, Cho incises the DNA. Then UvrC makes the 5' incision. The combined action of Cho and UvrC broadens the substrate range of nucleotide excision repair. 1181855211818552NucleoidNNCytoplasmicN/AyesyesNo420.1CYT0-0.200913-00="0"iCytoplasmic8.9614.25
VXIS_ECOLIP75970
xisE

vxis ymfG

vxis ymfGb1141 JW1127 Excisionase-like protein from lambdoid prophage 14CytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo120.127CYT0-0.200913-00="0"iUnknown2135.28
EXOX_ECOLIP0AEK0
exoX

yobC

yobCb1844 JW1833 Exodeoxyribonuclease 10 (EC 3.1.11.-) (Exodeoxyribonuclease X) (Exo X) (Exonuclease X)Nucleoid (Experimental) Capable of degrading both single-strand and double-strand DNA with 3' to 5' polarity. Has higher affinity for ssDNA ends than for dsDNA. 1051449610514496NucleoidNNCytoplasmicN/AyesyesNo500.182CYT0-0.200913-00="0"iCytoplasmic9.9712.29
EX7L_ECOLIP04994
xseA
b2509 JW2493 Exodeoxyribonuclease 7 large subunit (EC 3.1.11.6) (Exodeoxyribonuclease VII large subunit) (Exonuclease VII large subunit)Nucleoid (Experimental) Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides. It can also degrade 3' or 5' ss regions extending from the termini of duplex DNA molecules and displaced ss regions. 6284744 Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides. It can also degrade 3' or 5' ss regions extending from the termini of duplex DNA molecules and displaced ss regions. 30217566284744 3021756NucleoidNNCytoplasmCytoplasmicN/AnoyesNo330.128CYT0-0.200913-00="0"iCytoplasmic10
EX7S_ECOLIP0A8G9
xseB

yajE

yajEb0422 JW0412 Exodeoxyribonuclease 7 small subunit (EC 3.1.11.6) (Exodeoxyribonuclease VII small subunit) (Exonuclease VII small subunit)Nucleoid (Experimental) Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides. It can also degrade 3' or 5' ss regions extending from the termini of duplex DNA molecules and displaced ss regions. 30217563021756NucleoidNNCytoplasmCytoplasmicN/AnoyesNo280.099CYT0-0.200913-00="0"iCytoplasmic10
RECE_ECOLIP15032
recE
b1350 JW1344 Exodeoxyribonuclease 8 (EC 3.1.11.-) (Exodeoxyribonuclease VIII) (EXO VIII)In Bacillus subtilis RecE homolog was identified as part of a membrane DNA-complex.CytoplasmicAACell inner membrane | Peripheral membrane proteinCytoplasmicN/AProphage Integrase (EcoGene)nonoNo310.113CYT0-0.200913-00="0"iCytoplasmic8.9650.51
EX1_ECOLIP04995
sbcB

cpeA xonA

cpeA xonAb2011 JW1993 Exodeoxyribonuclease I (Exonuclease I) (EC 3.1.11.1) (DNA deoxyribophosphodiesterase) (dRPase)CytoplasmicAACytoplasmicN/AyesyesNo330.139CYT0-0.200913-00="0"iCytoplasmic9.9710.76
EX3_ECOLIP09030
xthA

xth

xthb1749 JW1738 Exodeoxyribonuclease III (EXO III) (Exonuclease III) (EC 3.1.11.2) (AP endonuclease VI)Nucleoid (Experimental) Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities. 89486518948651NucleoidNNCytoplasmicN/AyesyesNo210.116CYT0-0.200913-00="0"iCytoplasmic9.9724.05
EX5A_ECOLIP04993
recD
b2819 JW2787 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide)Nucleoid (Experimental) A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid and highly processive ATP-dependent bidirectional helicase (1350 bp/s). 35379613537961NucleoidNNCytoplasmicN/AyesyesNo340.142CYT0-0.200913-00="0"iCytoplasmic9.9715.37
EX5B_ECOLIP08394
recB

rorA

rorAb2820 JW2788 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) (Exodeoxyribonuclease V 135 kDa polypeptide)Nucleoid (Experimental) A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid and highly processive ATP-dependent bidirectional helicase (1350 bp/s). 35379613537961NucleoidNNCytoplasmicN/AyesyesNo500.115CYT0-0.200913-00="0"iCytoplasmic9.26
EX5C_ECOLIP07648
recC
b2822 JW2790 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) (Exodeoxyribonuclease V 125 kDa polypeptide)CytoplasmicAACytoplasmicN/AyesyesNo440.12CYT0-0.200913-00="0"iCytoplasmic9.975.7
PPX_ECOLIP0AFL6
ppx
b2502 JW2487 Exopolyphosphatase (ExopolyPase) (EC 3.6.1.11) (Metaphosphatase)Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane proteinCytoplasmicIM (predicted)yesyesNo290.112CYT0-0.200913-00="0"iCytoplasmic1047.13
RNB_ECOLIP30850
rnb
b1286 JW1279 Exoribonuclease 2 (EC 3.1.13.1) (Exoribonuclease II) (RNase II) (Ribonuclease II)Peripheral inner membrane protein facing the cytoplasm (Experimental) Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II PMID: 23344958 Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 23344958 PMID: 24438330Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N,F1CytoplasmCytoplasmicN/AyesyesNo230.181CYT0-0.200913-00="0"iCytoplasmic1048.82
EXUR_ECOLIP0ACL2
exuR
b3094 JW3065 Exu regulon transcriptional regulatorNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AyesyesNo400.109CYT0-0.200913-00="0"iCytoplasmic9.9722.94
FADR_ECOLIP0A8V6
fadR

oleR thdB

oleR thdBb1187 JW1176 Fatty acid metabolism regulator proteinNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmCytoplasmicN/AyesyesNo130.114CYT0-0.200913-00="0"iCytoplasmic8.9686.34
FADJ_ECOLIP77399
fadJ

yfcX

yfcXb2341 JW2338 Fatty acid oxidation complex subunit alpha [Includes: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC 4.2.1.17) (EC 5.1.2.3); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo700.113CYT0-0.200913-00="0"iCytoplasmic9.9715.86
FADB_ECOLIP21177
fadB

oldB

oldBb3846 JW3822 Fatty acid oxidation complex subunit alpha [Includes: Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC 4.2.1.17) (EC 5.1.2.3) (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/ATFyesyesNo310.13CYT0-0.200913-00="0"iCytoplasmic9.9715.16
FECE_ECOLIP15031
fecE
b4287 JW4247 Fe(3+) dicitrate transport ATP-binding protein fecE (Iron(III) dicitrate transport ATP-binding protein fecE)Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo620.148CYT0-0.200913-00="0"iCytoplasmicMembrane104.5
FECA_ECOLIP13036
fecA
b4291 JW4251 Fe(3+) dicitrate transport protein fecA (Iron(III) dicitrate transport protein fecA)Outer membrane protein (Experimental) (Probable) Outer membrane (Experimental) Outer Membrane ss-barrel protein (Experimental)Substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 2836368PMID: 15911532 PMID: 11266632 PMID: 11872840 PMID: 20932056 PMID: 2836368Outer Membrane b-barrel proteinHHSingle-pass type I membrane proteinOuter Membrane β-barrel protein (Experimental)N/ASECSURA,SKPBAM: β-Barrel Assembly MachinerynoyesYes340.831SpI3318.814-00Yn19-29c33/34oOuterMembrane101.41
FECC_ECOLIP15030
fecC
b4289 JW4249 Fe(3+) dicitrate transport system permease protein fecC (Iron(III) dicitrate transport system permease protein fecC)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo310.15TMH07.31223-89Yn8-27c36/37o60-81i93-111o117-140i152-171o177-194i201-220o240-270i282-304o310-328iCytoplasmicMembrane10
FECD_ECOLIP15029
fecD
b4288 JW4248 Fe(3+) dicitrate transport system permease protein fecD (Iron(III) dicitrate transport system permease protein fecD)Integral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo280.121TMH010.0321-77Yn4-19c31/32o55-76i85-103o109-126i138-158o226-256i268-290o296-315iCytoplasmicMembrane104.14
FECB_ECOLIP15028
fecB
b4290 JW4250 Fe(3+) dicitrate-binding periplasmic protein (Iron(III) dicitrate-binding periplasmic protein)Periplasmic (Experimental)PMID: 2651410PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECnoyesYes220.843SpI218.42911-00Yn7-16c21/22oPeriplasmic9.998.95
FBPC_ECOLIP37009
fbpC

afuC yagC

afuC yagCb0262 JW0254 Fe(3+) ions import ATP-binding protein FbpC (EC 3.6.3.30)Homologous to MalKPeripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AProphage Integrase (EcoGene)yesnoNo620.109CYT0-0.200913-00="0"iCytoplasmicMembrane7.8842.31
NFUA_ECOLIP63020
nfuA

gntY yhgI

gntY yhgIb3414 JW3377 Fe/S biogenesis protein nfuAContains Nif-like domains/nitrogen fixationCytoplasmicAACytoplasmicN/AyesyesNo380.123CYT0-0.200913-00="0"iCytoplasmic9.97
FENR_ECOLIP28861
fpr

mvrA

mvrAb3924 JW3895 Ferredoxin--NADP reductase (FNR) (EC 1.18.1.2) (DA1) (Flavodoxin reductase) (FLDR) (FLXR) (Methyl viologen resistance protein A)CytoplasmicAACytoplasmicN/AyesyesNo270.377CYT0-0.200913-00="0"iCytoplasmic9.9716.61
FIXX_ECOLIP68646
fixX

yaaT

yaaTb0044 JW0043 Ferredoxin-like protein fixXCytoplasmicAACytoplasmicN/AyesyesNo80.104CYT0-0.200913-00="0"iUnknown282.94
YDIT_ECOLIP77714
ydiT
b1700 JW1690 Ferredoxin-like protein ydiT 4Fe-4S ferredoxin-type, iron-sulphur binding domain (IPR017896) CytoplasmicAACytoplasmicN/AyesyesNo210.118CYT0-0.200913-00="0"iUnknown284.17
YGCO_ECOLIQ46905
ygcO
b2767 JW2737 Ferredoxin-like protein ygcO Iron binding CytoplasmicAACytoplasmicN/AyesyesNo200.119CYT0-0.200913-00="0"iUnknown2
NAPF_ECOLIP0AAL0
napF

yojG

yojGb2208 JW2196 Ferredoxin-type protein napFCytoplasmic (Experimental)PMID: 17074894CytoplasmicAACytoplasmic (Experimental)N/AnoyesNo430.117CYT0-0.200913-00="0"iCytoplasmic1012.39
NAPG_ECOLIP0AAL3
napG

yojA yojB

yojA yojBb2205 JW2193 Ferredoxin-type protein napGPeriplasmic (Experimental)TAT secretion system. PMID: 11967083PMID: 11967083PeriplasmicGGPeriplasmic (Experimental)N/ATATyesyesNo420.316SpI410.19393-00Yn21-32c41/42oCytoplasmic8.9620.35
NAPH_ECOLIP33934
napH

yejZ

yejZb2204 JW2192 Ferredoxin-type protein napHCell inner membrane (Probable)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo470.13TMH07.44595-44="0"i30-50o78-98i139-160o172-192iCytoplasmicMembrane106.33
FEPC_ECOLIP23878
fepC
b0588 JW0580 Ferric enterobactin transport ATP-binding protein fepCPeripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinCytoplasmicN/AyesyesNo630.171CYT0-0.200913-00="0"iCytoplasmicMembrane9.5917.69
FEPE_ECOLIP26266
fepE
b0587 JW0579 Ferric enterobactin transport protein fepEIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECnoyesNo460.11CYT0-0.200913-22="0"i42-61o339-362iCytoplasmicMembrane1033.53
FEPD_ECOLIP23876
fepD
b0590 JW0582 Ferric enterobactin transport system permease protein fepDIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo510.205TMH01.30983-99="0"o6-30i64-81o93-114i121-139o151-172i193-213o239-264i284-304o310-326iCytoplasmicMembrane10
FEPG_ECOLIP23877
fepG
b0589 JW0581 Ferric enterobactin transport system permease protein fepGIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo230.416TMH010.9363-88Yn8-23c41/42o65-83i95-112o118-138i145-167o187-211i236-262o274-296i303-324oCytoplasmicMembrane10
FHUF_ECOLIP39405
fhuF

yjjS

yjjSb4367 JW4331 Ferric iron reductase protein fhuFIt has a large transmembrane helix in the middle.PMID: 14756576Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCell membrane | Peripheral membrane proteinCytoplasmicIM (predicted)yesyesNo550.117CYT0-0.200913-01="0"i91-112oCytoplasmic1029.18
FUR_ECOLIP0A9A9
fur
b0683 JW0669 Ferric uptake regulation protein (Ferric uptake regulator)Nucleoid (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23138451NucleoidNNCytoplasmCytoplasmicN/AyesyesNo370.121CYT0-0.200913-00="0"iCytoplasmic10109.55
PAAB_ECOLIP76078
paaB

ynbF

ynbFb1389 JW1384 Ferrichrome-iron receptor (Ferric hydroxamate receptor) (Ferric hydroxamate uptake)CytoplasmicAACytoplasmicN/AnoyesNo300.214CYT0-0.200913-00="0"iUnknown286.06
FEPA_ECOLIP05825
fepA

fep feuB

fep feuBb0584 JW5086 Ferrienterobactin receptor (Enterobactin outer-membrane receptor) (Probable) Outer membrane (Probable) Outer membrane protein (Experimental) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544PMID: 11266632 PMID: 9886293 PMID: 20932056 PMID: 10806384 PMID: 10529396Outer Membrane b-barrel proteinHHCell outer membrane | Multi-pass membrane proteinOuter Membrane β-barrel protein (Experimental)N/ASECSkpBAM: β-Barrel Assembly MachineryyesyesYes230.836SpI2211.5401-00Yn7-17c22/23oOuterMembrane1011.34
FEPB_ECOLIP0AEL6
fepB
b0592 JW0584 Ferrienterobactin-binding periplasmic proteinPeriplasmic (Experimental)PMID: 10986237PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASECyesyesYes270.581SpI2613.3736-00Yn10-21c26/27oPeriplasmic1030.41
FTNA_ECOLIP0A998
ftnA

ftn gen-165 rsgA

ftn gen-165 rsgAb1905 JW1893 Ferritin-1 (EC 1.16.3.1)Copurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo480.159CYT0-0.200913-00="0"iCytoplasmic1074.86
FTNB_ECOLIP0A9A2
ftnB

yecI

yecIb1902 JW1890 Ferritin-like protein 2Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo480.152CYT0-0.200913-00="0"iCytoplasmic1082.57
HEMH_ECOLIP23871
hemH

popA visA

popA visAb0475 JW0464 Ferrochelatase (EC 4.99.1.1) (Heme synthase) (Protoheme ferro-lyase)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo630.136CYT0-0.200913-00="0"iCytoplasmic1015.62
FEOA_ECOLIP0AEL3
feoA
b3408 JW3371 Ferrous iron transport protein ACytoplasmicAACytoplasmicN/AnoyesNo430.132CYT0-0.200913-00="0"iUnknown2
FEOB_ECOLIP33650
feoB
b3409 JW3372 Ferrous iron transport protein BIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBout/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted), Detected in IM FractionSRPSECyesyesNo200.107CYT0-0.200913-109="0"o282-302i309-327o347-367i421-447o453-473i511-529o664-685i697-715o721-742iCytoplasmicMembrane108.56
FEOC_ECOLIP64638
feoC

yhgG

yhgGb3410 JW3373 Ferrous iron transport protein CCytoplasmicAACytoplasmicN/AnoyesNo200.174CYT0-0.200913-00="0"iUnknown2
FIEF_ECOLIP69380
fieF

yiiP

yiiPb3915 JW3886 Ferrous-iron efflux pump fieFIntegral inner membrane (Experimental)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo210.141TMH03.82255-66="0"i12-33o39-60i81-106o118-138i158-175o181-199iCytoplasmicMembrane10
SUFB_ECOLIP77522
sufB

ynhE

ynhEb1683 JW5273 FeS cluster assembly protein sufBPeripheral inner membrane protein (Probable)Completely soluble no evidence for hyrophobic patches. IM binding determined by immunolocalization.PMID: 11943156Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo310.117CYT0-0.200913-00="0"iUnknown243.02
SUFD_ECOLIP77689
sufD

ynhC

ynhCb1681 JW1671 FeS cluster assembly protein sufDPeripheral inner membrane protein (Probable)Forms complex with SufC and SufB.PMID: 10322040Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmicN/AyesyesNo670.127CYT0-0.200913-00="0"iCytoplasmic8.9636.65
FHUE_ECOLIP16869
fhuE
b1102 JW1088 FhuE receptor (Outer-membrane receptor for Fe(III)-coprogen, Fe(III)-ferrioxamine B and Fe(III)-rhodotrulic acid)Outer membrane protein (Experimental) Outer Membrane ss-barrel protein (Experimental)Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 3032906PMID: 10806384 PMID: 3032906Outer Membrane b-barrel proteinHHSingle-pass type I membrane proteinOuter Membrane β-barrel protein (Experimental)N/ASECBAM: β-Barrel Assembly MachinerynoyesYes370.821SpI3620.6615-00Yn19-30c36/37oOuterMembrane100.86
FIMZ_ECOLIP0AEL8
fimZ

ybcA

ybcAb0535 JW5073 Fimbriae Z proteinNucleoid (Experimental) Nucleoid (Potential) LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 23138451 NucleoidNNCytoplasmCytoplasmicN/AnoyesNo190.105CYT0-0.200913-00="0"iCytoplasmic9.9732.41
SFMF_ECOLIP38052
sfmF

ybcG

ybcGb0534 JW5072 Fimbrial-like protein sfmFPotential fimbrial sfm operon (class γ1); Fimbrial Subunit. Disulfide bridge (40 80) PeriplasmicGGPeriplasmPeriplasmicN/AfimbriaeSECCU: Chaperone Usher export systemnoyesYes210.763SpI2016.3129-00Yn4-15c20/21oExtracellular9.7164.72
MATB_ECOLIP0AAA3
matB

yagZ

yagZb0293 JW0287 Fimbrillin matB homologPeriplasmic (Experimental)Putative chaperone–usher fimbrial operon yag (class α); Fimbrial Subunit (yagZ). PMID: 16522795PMID: 16522795PeriplasmicGGPeriplasmic (Experimental)N/AfimbriaeSECnoyesYes230.863SpI2225.6343-00Yn5-15c22/23oUnknown2.534.62
FIMI_ECOLIP39264
fimI
b4315 JW5779 Fimbrin-like protein fimIFim operon (class γ1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Disulfide bridge (43 83) PeriplasmicGGPeriplasmPeriplasmicN/AfimbriaeSECCU: Chaperone Usher export systemnoyesYes200.716SpI1916.0857-00Yn6-13c19/20oExtracellular9.7237.53
FKBX_ECOLIP0AEM0
fkpB

slpA yaaD

slpA yaaDb0028 JW0026 FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase (PPIase) (EC 5.2.1.8) (Rotamase)CytoplasmicAACytoplasmicN/AyesyesNo260.119CYT0-0.200913-00="0"iCytoplasmic9.26109.75
FKBB_ECOLIP0A9L3
fklB

ytfC

ytfCb4207 JW5746 FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase (FKBP22) (PPIase) (EC 5.2.1.8) (Rotamase)CytoplasmicAACytoplasmPeriplasmCytoplasmicN/AyesyesNo460.205CYT0-0.200913-00="0"iCytoplasmic10
FKBA_ECOLIP45523
fkpA

yzzS

yzzSb3347 JW3309 FKBP-type peptidyl-prolyl cis-trans isomerase fkpA (PPIase) (EC 5.2.1.8) (Rotamase)Periplasmic (Experimental) (Experimental)SecB substrate. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350PMID: 9298646 PMID: 22030350PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASecBSECyesyesYes260.701SpI2514.7593-00Yn7-18c25/26oPeriplasmic10
SLYD_ECOLIP0A9K9
slyD
b3349 JW3311 FKBP-type peptidyl-prolyl cis-trans isomerase slyD (PPIase) (EC 5.2.1.8) (Histidine-rich protein) (Metallochaperone slyD) (Rotamase) (Sensitivity to lysis protein D) (WHP)Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Peripheral inner membrane protein facing the cytoplasmF1F1CytoplasmCytoplasmicN/AyesyesNo310.112CYT0-0.200913-00="0"iCytoplasmic10
FLGA_ECOLIP75933
flgA
b1072 JW1059 Flagella basal body P-ring formation protein flgAPeriplasmic (Experimental)Periplasmic chaperone for P ring protein. PMID: 12730325PMID: 12730325PeriplasmicGGSecretedPeriplasmic (Experimental)N/ASEC FlayesyesYes220.722SpI215.00523-00Yn8-16c21/22oPeriplasmic9.7612.09
FLGN_ECOLIP43533
flgN
b1070 JW1057 Flagella synthesis protein flgNSubstrate-specific chaperone of the flagellar export system along with FliT and FliS. Protects FlgK, FlgL from proteolysis. Interaction with FlhA which is an integral inner membrane protein isInvolved in efficient protein export. CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo240.105CYT0-0.200913-00="0"iCytoplasmic9.2681.47
FLIH_ECOLIP31068
fliH

fla AII.3 fla BIII

fla AII.3 fla BIIIb1940 JW1924 Flagellar assembly protein fliHCytoplasmicAACytoplasmCytoplasmicN/AnoyesNo170.104CYT0-0.200913-00="0"iCytoplasmic9.97109.4
FLGB_ECOLIP0ABW9
flgB

fla FII flbA

fla FII flbAb1073 JW1060 Flagellar basal-body rod protein flgB (Putative proximal rod protein)Periplasmic No signal Peptide (By similarity) Periplasmic (Experimental) Periplasm (By similarity)Proximal rod protein. PMID: 12730325 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 15136044PeriplasmicGGBacterial flagellum basal bodyPeriplasmic (Experimental)N/AflagellumFlagellum FlayesyesNo340.201CYT0-0.200913-00="0"iPeriplasmic1093.85
FLGC_ECOLIP0ABX2
flgC

fla FIII flaW

fla FIII flaWb1074 JW1061 Flagellar basal-body rod protein flgC (Putative proximal rod protein)Periplasmic No signal Peptide (By similarity) Periplasmic (Experimental) Periplasm (By similarity)Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGGBacterial flagellum basal bodyPeriplasmic (Experimental)N/AflagellumFlagellum FlayesyesNo320.201CYT0-0.200913-00="0"iPeriplasmic1043.37
FLGF_ECOLIP75938
flgF

fla FVI flaX

fla FVI flaXb1077 JW1064 Flagellar basal-body rod protein flgF (Putative proximal rod protein)Periplasmic (Experimental) Periplasm (By similarity)Homolog to flgG. Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGGBacterial flagellum basal bodyPeriplasmic (Experimental)N/AflagellumFlagellum FlayesyesNo300.199CYT0-0.200913-00="0"iPeriplasmic9.44101.77
FLGG_ECOLIP0ABX5
flgG

fla FVII flaL

fla FVII flaLb1078 JW1065 Flagellar basal-body rod protein flgG (Distal rod protein)Periplasmic No signal Peptide (By similarity) Periplasmic (Experimental) Periplasm (By similarity)Distal rod protein PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044PMID: 12631703 PMID: 12730325 PMID: 2181149 PMID: 15136044PeriplasmicGGBacterial flagellum basal bodyPeriplasmic (Experimental)N/AflagellumFlagellum FlayesyesNo240.123CYT0-0.200913-00="0"iExtracellular1078.81
FLHA_ECOLIP76298
flhA
b1879 JW1868 Flagellar biosynthesis protein flhACell inner membrane (Probable)Part of FlgN-FlgK-FliJ-FlhA complex.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo240.137TMH010.1028-78="0"i21-40o46-65i77-97o117-140i209-231o251-273i285-305o311-327iCytoplasmicMembrane1014.28
FLHB_ECOLIP76299
flhB

yecQ

yecQb1880 JW1869 Flagellar biosynthetic protein flhBIntegral inner membrane (Experimental)Regulates ordered export of flagellar components via autocleavage mechanism.PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinIntegral Membrane ProteinIM (predicted)SRPSECyesyesNo540.142CYT0-0.200913-44="0"i35-53o88-115i145-168o188-213iCytoplasmicMembrane10
FLIP_ECOLIP0AC05
fliP

flaR

flaRb1948 JW1932 Flagellar biosynthetic protein fliPPolytopic inner membrane (Probable) Cell inner membrane, Bacterial flagellum basal body (Probable)A cleavable signal peptide is required for the full function of FliP protein.PMID: 15194505 PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinBacterial flagellum basal bodyIntegral Membrane ProteinIM (predicted), Detected in IM FractionflagellumSRPSECnoyesYes220.692TMH013.015-54Yn4-15c21/22o45-74i86-105o185-211i223-244oCytoplasmicMembrane10
FLIQ_ECOLIP0AC07
fliQ

flaQ

flaQb1949 JW1933 Flagellar biosynthetic protein FliQIntegral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinBacterial flagellum basal bodyIntegral Membrane ProteinIM (predicted)flagellumSRPSECnoyesNo410.283TMH014.373-21Yn16-35c40/41o56-74iCytoplasmicMembrane1030.83
FLIR_ECOLIP33135
fliR

flaP

flaPb1950 JW1934 Flagellar biosynthetic protein fliRCell inner membrane, Bacterial flagellum basal body (Probable)PMID: 15919996Integral Inner Membrane BBout/outCell inner membrane | Multi-pass membrane proteinBacterial flagellum basal bodyIntegral Membrane ProteinIM (predicted)flagellumSRPSECnoyesNo610.121TMH05.44162-66="0"o15-33i42-59o65-86i131-151o179-201i213-237oCytoplasmicMembrane10
YCGR_ECOLIP76010
ycgR
b1194 JW1183 Flagellar brake protein YcgR (Cyclic di-GMP binding protein YcgR)Peripheral inner membrane protein facing the cytoplasm (Probable) Bacterial flagellum basal body (Probable)Acts as a "backstop brake" to control flagellar motor direction and speed to affect chemotaxis.PMID: 20346719 PMID: 20303158Peripheral inner membrane protein facing the cytoplasmF1F1Bacterial flagellum basal bodyCytoplasmicN/AflagellumnoyesNo600.155CYT0-0.200913-00="0"iCytoplasmic8.9638.72
FLIJ_ECOLIP52613
fliJ

flaO flaS

flaO flaSb1942 JW1926 Flagellar fliJ proteinFlagellum (Probable)Part of FlgN-FlgK-FliJ-FlhA complex.PMID: 22233518Peripheral inner membrane protein facing the cytoplasmF1F1Cell membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicN/AnoyesNo360.121CYT0-0.200913-00="0"iCytoplasmic1089.76
FLGE_ECOLIP75937
flgE

fla FV flaK

fla FV flaKb1076 JW1063 Flagellar hook protein flgECell surface appendage (Experimental)Hook protein. PMID: 12730325PMID: 12730325Peripheral outer membrane protein facing the extra-cellular spaceF4F4Bacterial flagellum basal bodyCell surface appendage (Experimental)N/AflagellumFlagellum Flapseudogene (Ochman & Davalos, 2006)noyesNo280.186CYT0-0.200913-00="0"iExtracellular10106.24
FLGK_ECOLIP33235
flgK

flaS flaW

flaS flaWb1082 JW1069 Flagellar hook-associated protein 1 (HAP1)Outer membrane vesicles (OMVs) (Probable) Cell surface appendage (Experimental)Part of FlgN-FlgK-FliJ-FlhA complex. First hook-filament junction protein. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type flgK, an E. coli strain with a flgK-null mutation produced reduced amounts of OMVs. PMID: 12730325PMID: 24134841 PMID: 12730325Peripheral outer membrane protein facing the extra-cellular spaceF4F4SecretedBacterial flagellumCell surface appendage (Experimental)N/AflagellumFlagellum FlayesyesNo180.238SpI172.83622-00="0"iExtracellular9.9643.51
FLID_ECOLIP24216
fliD

flaV flbC

flaV flbCb1924 JW1909 Flagellar hook-associated protein 2 (HAP2) (Filament cap protein) (Flagellar cap protein)Cell surface appendage (Experimental)Filament capping protein. PMID: 7551038 PMID: 1527488 PMID: 6305924PMID: 7551038 PMID: 1527488 PMID: 6305924Peripheral outer membrane protein facing the extra-cellular spaceF4F4SecretedBacterial flagellumCell surface appendage (Experimental)N/AflagellumFlagellumFlanoyesNo250.12CYT0-0.200913-00="0"iExtracellular1093.72
FLGL_ECOLIP29744
flgL

flaT flaU

flaT flaUb1083 JW1070 Flagellar hook-associated protein 3 (HAP3) (Hook-filament junction protein)Cell surface appendage (Experimental)Second hook-filament junction protein. PMID: 12730325PMID: 12730325Peripheral outer membrane protein facing the extra-cellular spaceF4F4SecretedBacterial flagellumCell surface appendage (Experimental)N/AflagellumFlagellum FlayesyesNo580.149CYT0-0.200913-00="0"iExtracellular1035.39
FLIE_ECOLIP0A8T5
fliE

fla AI flaN

fla AI flaNb1937 JW1921 Flagellar hook-basal body complex protein fliEInner membrane (Probable)MS-ring rod junction proteinPMID: 10809679Peripheral inner membrane protein facing the periplasmF2F2Bacterial flagellum basal bodyCytoplasmicN/AflagellumFlagellum noyesNo410.269CYT0-0.200913-00="0"iPeriplasmic1062.41
FLIK_ECOLIP52614
fliK

flaE flaR

flaE flaRb1943 JW1927 Flagellar hook-length control proteinCytoplasmic, Extra-cellular (Probable)Hook-length control protein. Protein FliK detects the point where the flagellar hook substructure has reached its optimal length. Then it terminates hook export and assembly and transmits a signal to begin filament export.PMID: 17456739Cytoplasmic, Extra-cellularA, XA,XCytoplasmicN/AflagellumFlagellumFlanoyesNo470.186CYT0-0.200913-00="0"iExtracellular1097.34
FLGH_ECOLIP0A6S0
flgH

fla FVIII flaY

fla FVIII flaYb1079 JW5153 Flagellar L-ring protein (Basal body L-ring protein)Outer membrane Lipoprotein (Experimental) Outer membrane protein (Experimental) Proven pignal peptidase II substrate. L ring protein. PMID: 15174130PMID: 15174130 PMID: 20932056Outer Membrane LipoproteinIICell outer membrane | Lipid-anchorBacterial flagellum basal bodyOuter membrane Lipoprotein (Experimental)N/AflagellumSECLOL: Localization Of LipoproteinsyesyesYes240.235SpII2117.0713Pos+2=A00Yn14-25c33/34oOuterMembrane1024.76
FLIF_ECOLIP25798
fliF

fla AII.1 fla BI

fla AII.1 fla BIb1938 JW1922 Flagellar M-ring proteinCell inner membrane, Bacterial flagellum basal body (Probable)PMID: 15919996Integral Inner Membrane BBin/inCell inner membrane | Multi-pass membrane proteinBacterial flagellum basal bodyIntegral Membrane ProteinIM (predicted), Detected in IM FractionflagellumSRPSECnoyesNo460.141SpI433.78777-22="0"i23-43o451-468iCytoplasmicMembrane1054.53
FLIG_ECOLIP0ABZ1
fliG

fla AII.2 fla BII

fla AII.2 fla BIIb1939 JW1923 Flagellar motor switch protein FliGPeripheral inner membrane protein facing the cytoplasm (Experimental) Membrane associated (Experimental)Flaggelar C-ring proteins - FliG, FliM, and FliN. MotA and MotB are ion-conducting membrane proteins that form the stator of the flagellum motor. FliG is a component of the rotor, present in about 25 copies per flagellum. The C-terminal domain of FliG is a component of the rotor in the bacterial flagellar motor. PMID: 10440379 PMID: 17085573 PMID: 16077109 PMID: 8631704PMID: 10440379 PMID: 17085573 PMID: 16077109 PMID: 8631704Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane protein | Cytoplasmic sideBacterial flagellum basal bodyMembrane associated (Experimental)Soluble protein detected in Inner Membrane FractionflagellumnoyesNo230.251CYT0-0.200913-00="0"iCytoplasmic9.1280.75
FLIM_ECOLIP06974
fliM

cheC2 fla AII fla QII

cheC2 fla AII fla QIIb1945 JW1929 Flagellar motor switch protein FliMFlaggelar C-ring proteins - FliG, FliM, and FliN.Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane proteinBacterial flagellum basal bodyCytoplasmicIM (predicted)flagellumnoyesNo100.109CYT0-0.200913-00="0"iCytoplasmicMembrane1057.89
FLIN_ECOLIP15070
fliN

flaN motD

flaN motDb1946 JW1930 Flagellar motor switch protein FliNFlaggelar C-ring proteins - FliG, FliM, and FliN.Peripheral inner membrane protein facing the cytoplasmF1F1Cell inner membrane | Peripheral membrane protein | Cytoplasmic sideBacterial flagellum basal bodyCytoplasmicN/AflagellumnoyesNo350.119CYT0-0.200913-00="0"iCytoplasmicMembrane10107.99
FLGI_ECOLIP0A6S3
flgI

fla FIX flaM

fla FIX flaMb1080 JW1067 Flagellar P-ring protein (Basal body P-ring protein)Periplasmic (Experimental) (Experimental)P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024PMID: 12730325 PMID: 15838024PeriplasmicGGPeriplasmBacterial flagellum basal bodyPeriplasmic (Experimental)N/AflagellumSRP (exp)SECDsbAFlanoyesYes200.783SpI1915.6262-00Yn4-14c19/20oPeriplasmic1063.67
FLHE_ECOLIP76297
flhE
b1878 JW1867 Flagellar protein flhEPart of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544PeriplasmicGGPeriplasmicN/ASECFlayesyesYes170.511SpI168.24229-00Yn3-14c26/27oUnknown2.568.14
FLIL_ECOLIP0ABX8
fliL

cheC1 fla AI fla QI

cheC1 fla AI fla QIb1944 JW1928 Flagellar protein FliLIntegral Inner Membrane BBin/outCell inner membrane | Single-pass membrane proteinOuter membrane LipoproteinSoluble protein detected in Inner Membrane FractionSRPSECnoyesNo430.268SpII2610.5269Pos+2=A11="0"i14-35oCytoplasmicMembrane1035.39
FLIO_ECOLIP22586
fliO

flaP flbD

flaP flbDb1947 JW5316 Flagellar protein fliOIntegral Inner Membrane BBout/inCell membrane | Single-pass membrane proteinBacterial flagellum basal bodyMembrane anchoredIM (predicted)flagellumSRPSECnoyesNo270.161TMH00.0866025-11="0"o18-39iCytoplasmicMembrane9.9714.69
FLIS_ECOLIP26608
fliS
b1925 JW1910 Flagellar protein fliSCytoplasmicAACytoplasm cytosolCytoplasmicN/AnoyesNo120.136CYT0-0.200913-00="0"iCytoplasmic9.2668.87
FLIT_ECOLIP0ABY2
fliT
b1926 JW1911 Flagellar protein fliTCytoplasmicAACytoplasmCytosolCytoplasmicN/AnoyesNo510.107CYT0-0.200913-00="0"iCytoplasmic9.2693.25
FLK_ECOLIP15286
flk

div

divb2321 JW2318 Flagellar regulator flkIntegral Inner Membrane BBout/inCell inner membrane | Single-pass membrane proteinMembrane anchoredIM (predicted)SRPSECyesyesNo540.104CYT0-0.200913-11="0"o313-330iCytoplasmicMembrane9.8261.56
FLHC_ECOLIP0ABY7
flhC

flaI

flaIb1891 JW1880 Flagellar transcriptional activator flhCNucleoid (Experimental)PMID: 23138451NucleoidNNCytoplasmicN/AnoyesNo340.108CYT0-0.200913-00="0"iCytoplasmic102.88
FLIC_ECOLIP04949
fliC

flaF hag

flaF hagb1923 JW1908 FlagellinOuter membrane vesicles (OMVs) (Probable) Cell surface appendage (Experimental)Flameint protein, flagellin. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type fliC, an E. coli strain with a fliC-null mutation produced reduced amounts of OMVs based on both protein and phosphate levels. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 12730325PMID: 24134841 PMID: 12730325Peripheral outer membrane protein facing the extra-cellular spaceF4F4SecretedBacterial flagellumCell surface appendage (Experimental)Soluble protein detected in Inner Membrane FractionflagellumFlagellumFlanoyesNo520.13CYT0-0.200913-00="0"iExtracellular1080.39
FLII_ECOLIP52612
fliI

fla AIII flaC

fla AIII flaCb1941 JW1925 Flagellum-specific ATP synthase (EC 3.6.3.14)CytoplasmicAACytoplasmCytoplasmicN/AnoyesNo210.14CYT0-0.200913-00="0"iCytoplasmic9.2612.85
FLAV_ECOLIP61949
fldA
b0684 JW0671 Flavodoxin-1CytoplasmicAACytoplasmicN/AyesyesNo630.119CYT0-0.200913-00="0"iCytoplasmic8.9698.74
FLAW_ECOLIP0ABY4
fldB
b2895 JW2863 Flavodoxin-2CytoplasmicAACytoplasmicN/AyesyesNo180.158CYT0-0.200913-00="0"iCytoplasmic8.9690.54
HMP_ECOLIP24232
hmp

fsrB hmpA

fsrB hmpAb2552 JW2536 Flavohemoprotein (Flavohemoglobin) (HMP) (Hemoglobin-like protein) (Nitric oxide dioxygenase) (NO oxygenase) (NOD) (EC 1.14.12.17)Cytoplasm (Probable)PMID: 7875569CytoplasmicAACytoplasmCytoplasmic(experimental)N/AyesyesNo210.115CYT0-0.200913-00="0"iCytoplasmic1087.08
WRBA_ECOLIP0A8G6
wrbA
b1004 JW0989 Flavoprotein wrbA (Trp repressor-binding protein) (Experimental) Potential substrate of periplasmic chaperone YfgM). PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562PMID: 25403562CytoplasmicAACytoplasmicIM (predicted)YfgMnoyesNo220.147CYT0-0.200913-00="0"iUnknown276.94
SSUE_ECOLIP80644
ssuE

ycbP

ycbPb0937 JW0920 FMN reductase (EC 1.5.1.29) (Sulfate starvation-induced protein 4) (SSI4)CytoplasmicAACytoplasmicN/A