entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermsubloci_uniprotsubloci_echoLbernselorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
CSGF_ECOLIP0AE98
csgF
b1038 JW1021 Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Curli production assembly/transport component CsgFPeripheral outer membrane protein facing the extra-cellular space (Probable) Peripheral outer membrane protein facing the extra-cellular space (Experimental)Curli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475PMID: 16522795 PMID: 21645131 PMID: 19131513 PMID: 9928475Peripheral outer membrane protein facing the extra-cellular spaceF4F4Cell outer membrane Periplasmic (Experimental)N/ACurliSECCurliyesyesYes200.853SpI1913.9164-10Yn6-14c19/20oExtracellular930
CSGA_ECOLIP28307
csgA
b1042 JW1025 Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Major curlin subunit CsgAPeripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)Curli component. CsgA, is secreted from the cell in an unpolymerized form PMID: 11823641 The secretion and stability of CsgA and CsgB are dependent on the outer membrane lipoprotein CsgG. PMID: 21645131 Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041 During a process called interbacterial complementation, a csgB mutant cell secretes soluble CsgA that can be assembled on the surface of a cell expressing only csgB PMID: 8817489 High levels of both IbsC and ShoB lead to changes in membrane potential suggesting that both act at the membrane, consistent with the hydrophobic nature of the proteins.PMID: 11823641 PMID: 21645131 PMID: 9383186 PMID: 9312041 PMID: 8817489 PMID: 18710431Peripheral outer membrane protein facing the extra-cellular spaceF4F4Fimbrium Cell surface appendage (Experimental)N/ACurliSEC CurliyesyesYes210.854SpI2020.7833-00Yn3-14c20/21oExtracellular966
CSGB_ECOLIP0ABK7
csgB
b1041 JW1024 Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Minor curlin subunit CsgBPeripheral outer membrane protein facing the extra-cellular space (Probable) Peripheral outer membrane protein facing the extra-cellular space (Experimental)Curli component. CsgB localization requires at least one component of the curli secretion apparatus, CsgF. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041PMID: 19131513 PMID: 9383186 PMID: 9312041Peripheral outer membrane protein facing the extra-cellular spaceF4F4Fimbrium Cell surface appendage (Experimental)N/ACurliSECCurliyesyesYes220.53SpI2116.29-00Yn5-13c21/22oExtracellular9
CSGG_ECOLIP0AEA2
csgG
b1037 JW1020 Curli related OM lipoprotein & extra-cellular peripheral OM protein (SEC through IM), inserted in OM by LOL), Curli production assembly/transport component CsgGOuter Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Probable)Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130PMID: 15174130 PMID: 21645131Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular spaceI, F4I,F4Cell membrane Outer membrane Lipoprotein (Experimental)N/ACurliSECLOL: Localization Of LipoproteinsyesyesYes200.544SpII1512.9163Pos+2=L00Yn4-13c18/19oCytoplasmicMembrane944
CSGE_ECOLIP0AE95
csgE
b1039 JW1022 Curli related periplasmic protein (SEC through IM), Curli production assembly/transport component CsgEPeriplasmic (Experimental)Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475PMID: 9928475PeriplasmicGGPeriplasmic (Experimental)N/ACurliSECSurAyesyesYes230.789SpI223.89784-00Yn7-17c22/23oUnknown215
SECM_ECOLIP62395
secM

srrA yacA

srrA yacAb0097 JW5007 cytoplasmic & periplasmic protein (SEC through IM), Secretion monitor SecMCytoplasmic, Periplasmic (Probable) Cytoplasmic, Periplasmic (Experimental)SecM regulates secA expression by translational coupling of the secM secA operon. Fusion of SecM with alkaline phosphatase activity exhibits substantial activity in the periplasm. SecM periplasmic form is rapidly degraded. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 11597451PMID: 1834634 PMID: 11597451Cytoplasmic, PeriplasmicA, GA,GCytoplasm; cytosol Periplasmic (Experimental)N/ASECSurAyesyesYes380.527SpI3711.5151-10Yn20-31c37/38oCytoplasmic10
YGFZ_ECOLIP0ADE8
ygfZ

yzzW

yzzWb2898 JW2866 cytoplasmic & periplasmic protein (SEC through IM), tRNA-modifying YgfZ Cytoplasmic, Periplasmic (By similarity) Cytoplasmic, Periplasmic (Experimental)Subcellular localization analysis of cytotoxic necrotizing factor 1 <a href="http://www.uniprot.org/uniprot/Q1R2U0">(CNF1)</a> demonstrated that YgfZ, a periplasmic protein, contributes to secretion of CNF1 (a normally cytoplasmic protein) into outer-membrane vesicles (OMVs) in pathogenic E.coli strain RS218 O18:K1:H7 PMID: 22174383. PMID: 9298646PMID: 22174383 PMID: 9298646Cytoplasmic, PeriplasmicA, GA,GCytoplasm Cytoplasmic (Experimental)N/ASECyesyesNo310.252CYT0-0.200913-00NiUnknown282
PMBA_ECOLIP0AFK0
pmbA

tldE

tldEb4235 JW4194 cytoplasmic metalloprotease PmbA CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo360.159CYT0-0.200913-00NiCytoplasmic1016
TLDD_ECOLIP0AGG8
tldD

yhdO

yhdOb3244 JW3213 cytoplasmic metalloprotease TldD CytoplasmicAACytoplasmicN/AyesyesNo130.163CYT0-0.200913-00NiCytoplasmic990
ARCA_ECOLIP0A9Q1
arcA

cpxC dye fexA msp seg sfrA

cpxC dye fexA msp seg sfrAb4401 JW4364 cytoplasmic nucleoid peripheral IM protein, Aerobic respiration control ArcACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 18792681 PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AyesyesNo280.105CYT0-0.200913-00NiCytoplasmic1090
SYA_ECOLIP00957
alaS

lovB

lovBb2697 JW2667 cytoplasmic nucleoid peripheral IM protein, Alanine--tRNA ligase AlaSCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicN/ATFnoyesNo260.129CYT0-0.200913-00NiCytoplasmic957
RHLB_ECOLIP0A8J8
rhlB

mmrA

mmrAb3780 JW3753 cytoplasmic nucleoid peripheral IM protein, ATP-dependent RNA helicase RhlBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 18337249 PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AyesyesNo560.132CYT0-0.200913-00NiCytoplasmic9
PUTA_ECOLIP09546
putA

poaA

poaAb1014 JW0999 cytoplasmic nucleoid peripheral IM protein, Bifunctional PutACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)The proline dehydrogenase PutA protein from S. typhimurium was shown to act as a membrane-associated enzyme or as a transcriptional repressor PMID: 8483946. Catabolism of proline requires PutA to associate with the membrane because reoxidation of the FAD cofactor in PutA needs the presence of an electron acceptor. Repression of the put operon requires PutA to bind to the put control-region DNA in the cytoplasm. Transcription factor PMID: 23138451. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 8483946 PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo640.118CYT0-0.200913-00NiCytoplasmic9
CRP_ECOLIP0ACJ8
crp

cap csm

cap csmb3357 JW5702 cytoplasmic nucleoid peripheral IM protein, cAMP-activated global transcriptional regulator CrpCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation.PMID: 15650111 PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/ATFyesyesNo240.105CYT0-0.200913-00NiCytoplasmic9
DNAA_ECOLIP03004
dnaA
b3702 JW3679 cytoplasmic nucleoid peripheral IM protein, Chromosomal replication initiator DnaACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)C-terminal amphiphilic helix (355-370). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 10660626 PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicN/AnoyesNo320.146CYT0-0.200913-00NiCytoplasmic1038
MUKB_ECOLIP22523
mukB
b0924 JW0907 cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 16982609 PMID: 20921377 PMID: 11886550 PMID: 22380631 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo460.13CYT0-0.200913-00NiCytoplasmic10
MUKE_ECOLIP22524
mukE

kicA ycbA

kicA ycbAb0923 JW0906 cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukECytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 16982609 PMID: 20921377 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo250.113CYT0-0.200913-00NiCytoplasmic985
MUKF_ECOLIP60293
mukF

kicB

kicBb0922 JW0905 cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukFCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 16982609 PMID: 20921377 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo370.127CYT0-0.200913-00NiCytoplasmic1076
CSGD_ECOLIP52106
csgD
b1040 JW1023 cytoplasmic nucleoid peripheral IM protein, CsgBAC operon transcriptional regulatory protein CsgDCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. CsgD despite its function as a transcription activator, is localized in the cytoplasmic membrane. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 20874755 PMID: 16513732 PMID: 23138451 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cell inner membrane CytoplasmicN/AyesyesNo260.267CYT0-0.200913-00NiCytoplasmic920
GYRA_ECOLIP0AES4
gyrA

hisW nalA parD

hisW nalA parDb2231 JW2225 cytoplasmic nucleoid peripheral IM protein, DNA gyrase subunit A GyrACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/ATFyesyesNo360.106CYT0-0.200913-00NiCytoplasmic949
GYRB_ECOLIP0AES6
gyrB

acrB cou himB hisU nalC parA pcbA

acrB cou himB hisU nalC parA pcbAb3699 JW5625 cytoplasmic nucleoid peripheral IM protein, DNA gyrase subunit B GyrBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/ATFyesyesNo540.109CYT0-0.200913-00NiCytoplasmic9
DPO1_ECOLIP00582
polA

resA

resAb3863 JW3835 cytoplasmic nucleoid peripheral IM protein, DNA polymerase I PolACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AnoyesNo590.268CYT0-0.200913-00NiCytoplasmic976
DPS_ECOLIP0ABT2
dps

pexB vtm

pexB vtmb0812 JW0797 cytoplasmic nucleoid peripheral IM protein, DNA protection during starvation DpsCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoid-associated protein (NAP) Dodecameric Dps forms a protein cage with a hollow interior that sequesters Fe 2+ and reduces hydroxyl radical formation PMID: 23701194 PMID: 7984106PMID: 21541338 PMID: 23230279 PMID: 7984106Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoidCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo530.125CYT0-0.200913-00NiCytoplasmic1044
RADA_ECOLIP24554
radA

sms

smsb4389 JW4352 cytoplasmic nucleoid peripheral IM protein, DNA repair RadACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AyesyesNo410.199CYT0-0.200913-00NiCytoplasmicMembrane929
RECF_ECOLIP0A7H0
recF

uvrF

uvrFb3700 JW3677 cytoplasmic nucleoid peripheral IM protein, DNA replication and repair RecFCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. The prokaryotic protein recF is a single-stranded DNA-binding protein which also probably binds ATP. RecF is involved in DNA metabolism; it is required for recombinational DNA repair and for induction of the SOS response. PMID: 1542576PMID: 19766000 PMID: 1542576Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo320.128CYT0-0.200913-00NiCytoplasmic96
PARC_ECOLIP0AFI2
parC
b3019 JW2987 cytoplasmic nucleoid peripheral IM protein, DNA topoisomerase 4 subunit A ParCCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Dimer of topoisomerase IV subunit A <a href="http://BioCyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10686-MONOMER">(bioCyc)</a> Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1334483 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cell membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo180.118CYT0-0.200913-00NiCytoplasmicMembrane1017
FIS_ECOLIP0A6R3
fis
b3261 JW3229 cytoplasmic nucleoid peripheral IM protein, DNA-binding FisCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoid-associated protein (NAP).PMID: 21541338 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo190.116CYT0-0.200913-00NiCytoplasmic9110
HNS_ECOLIP0ACF8
hns

bglY cur drdX hnsA msyA osmZ pilG topS

bglY cur drdX hnsA msyA osmZ pilG topSb1237 JW1225 cytoplasmic nucleoid peripheral IM protein, DNA-binding H-NS Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo190.173CYT0-0.200913-00NiCytoplasmic10103
DBHA_ECOLIP0ACF0
hupA
b4000 JW3964 cytoplasmic nucleoid peripheral IM protein, DNA-binding HU-alpha HupACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338. Bacterial histone-like DNA-binding proteins signature (<a href="http://prosite.expasy.org/PDOC00044" targer="_blank">PDOC00044</a>).PMID: 21541338 PMID: 23230279 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo240.244CYT0-0.200913-00NiCytoplasmic996
DBHB_ECOLIP0ACF4
hupB

hopD

hopDb0440 JW0430 cytoplasmic nucleoid peripheral IM protein, DNA-binding HU-beta HupBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338. Bacterial histone-like DNA-binding proteins signature PMID: 21541338 PMID: 23230279 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AyesyesNo270.114CYT0-0.200913-00NiCytoplasmic9
STPA_ECOLIP0ACG1
stpA

hnsB

hnsBb2669 JW2644 cytoplasmic nucleoid peripheral IM protein, DNA-binding StpACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338.PMID: 21541338 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AyesyesNo190.184CYT0-0.200913-00NiCytoplasmic915
RPOA_ECOLIP0A7Z4
rpoA

pez phs sez

pez phs sezb3295 JW3257 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit alpha RpoACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23230279 PMID: 21541338Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AyesyesNo250.163CYT0-0.200913-00NiCytoplasmic9
RPOB_ECOLIP0A8V2
rpoB

groN nitB rif ron stl stv tabD

groN nitB rif ron stl stv tabDb3987 JW3950 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit beta RpoBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/ATFyesyesNo400.117CYT0-0.200913-00NiCytoplasmic9
RPOC_ECOLIP0A8T7
rpoC

tabB

tabBb3988 JW3951 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit beta' RpoCCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/ATFyesyesNo260.118CYT0-0.200913-00NiCytoplasmic9
RPOZ_ECOLIP0A800
rpoZ
b3649 JW3624 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit omega RpoZCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)The presence of omega subunit of RNA polymerase (RpoZ) is necessary for maturation of RNA polymerase. Experimentally verified gene-product. Methionine start codon is cleaved.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AyesyesNo350.113CYT0-0.200913-00NiCytoplasmic881
HDA_ECOLIP69931
hda

idaB yfgE

idaB yfgEb2496 JW5397 cytoplasmic nucleoid peripheral IM protein, DnaA regulatory inactivator HdaCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)Homolgous to DnaA and more specific to C-terminal anchor of DnaA.PMID: 12618445Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo330.157CYT0-0.200913-00NiCytoplasmic9
RPOE_ECOLIP0AGB6
rpoE

sigE

sigEb2573 JW2557 cytoplasmic nucleoid peripheral IM protein, ECF RNA polymerase sigma-E factor RpoECytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity)SigmaE controls the ??E-dependent extracytoplasmic stress response and is required to maintain cell envelope integrity in Escherichia coli PMID: 9159523 PMID: 18253509. RseA, encodes an anti-sigma(E) activity demonstrated at both the genetic and biochemical levels PMID: 9159522. RseC is an inner membrane protein that positively modulates sigma(E) activity PMID: 9159522. ??E is held at the membrane by the antisigma factor RseA. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB binds to the periplasmic domain of RseA and protects RseA from proteolysis. PMID: 17360428. Unfolded OMPs activate the protease DegS, which cleave RpoE regulon PMID: 15520285. Identified in the inner membrane by biochemical followed by two-dimensional gel electrophoresis and tandem mass spectrometry PMID: 15911532. PMID: 15774872 PMID: 16597971 PMID: 11777003 Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase. PMID: 12718891 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 9159522 PMID: 15911532 PMID: 20971899 PMID: 23138451 PMID: 15520285 PMID: 7889934 PMID: 17360428 PMID: 11777003 PMID: 15774872 PMID: 16597971 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo440.111CYT0-0.200913-00NiCytoplasmic10101
RNB_ECOLIP30850
rnb
b1286 JW1279 cytoplasmic nucleoid peripheral IM protein, Exoribonuclease 2 RnbCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II PMID: 23344958 Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 23344958 PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicN/AyesyesNo230.181CYT0-0.200913-00NiCytoplasmic1048
CYSB_ECOLIP0A9F3
cysB
b1275 JW1267 cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator CysBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicIM (predicted)yesyesNo240.141CYT0-0.200913-00NiCytoplasmic108
MALT_ECOLIP06993
malT

malA

malAb3418 JW3381 cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator MalTCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the MalK peripheral ATPase of the maltose transporter. Trigger factor-sensitive aggregation. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 15063852 PMID: 23138451 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/ATFyesyesNo360.12CYT0-0.200913-00NiUnknown78
MLRA_ECOLIP33358
mlrA

yehV

yehVb2127 JW2115 cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator MlrACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)MlrA has regulatory role in transcription activation of csgD, which is the master regulator of biofilm formation.PMID: 20874755Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicIM (predicted)yesyesNo230.15CYT0-0.200913-01No135-155iCytoplasmic9114
IHFA_ECOLIP0A6X7
ihfA

hid himA

hid himAb1712 JW1702 cytoplasmic nucleoid peripheral IM protein, Integration host factor subunit alpha IhfACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Translation. Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)PMID: 21541338. Bacterial histone-like DNA-binding proteins signature PMID: 21541338 PMID: 23230279 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm; nucleoid CytoplasmicN/AnoyesNo240.109CYT0-0.200913-00NiCytoplasmic9
IHFB_ECOLIP0A6Y1
ihfB

himD hip

himD hipb0912 JW0895 cytoplasmic nucleoid peripheral IM protein, Integration host factor subunit beta IhfBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Translation, nucleoid-associated protein (NAP). Bacterial histone-like DNA-binding proteins signature PMID: 21541338 PMID: 23230279 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicN/AnoyesNo390.116CYT0-0.200913-00NiCytoplasmic923
LRP_ECOLIP0ACJ0
lrp

alsB ihb livR oppI

alsB ihb livR oppIb0889 JW0872 cytoplasmic nucleoid peripheral IM protein, Leucine-responsive regulatory protein LrpCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicIM (predicted)yesyesNo480.101CYT0-0.200913-00NiCytoplasmic936
MATP_ECOLIP0A8N0
matP

ycbG

ycbGb0956 JW0939 cytoplasmic nucleoid peripheral IM protein, Macrodomain Ter protein MatPCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 18984159Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmN/AyesyesNo370.125CYT0-0.200913-00NiCytoplasmic892
MLC_ECOLIP50456
mlc
b1594 JW1586 cytoplasmic nucleoid peripheral IM protein, MlcCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Cytoplasmic global repressor of gene transcription that is recruited to the inner membrane where it binds the PtsB protein after the latter has been phosphorylated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15255904 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicIM (predicted)yesyesNo430.124CYT0-0.200913-00NiCytoplasmic918
YBBO_ECOLIP0AFP4
ybbO
b0493 JW0482 cytoplasmic nucleoid peripheral IM protein, Oxidoreductase YbbOCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo450.12CYT0-0.200913-01No240-258iCytoplasmic9
PHOB_ECOLIP0AFJ5
phoB
b0399 JW0389 cytoplasmic nucleoid peripheral IM protein, Phosphate regulon transcriptional regulatory PhoB Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PhoB is a dual transcription regulator that activates expression of the Pho regulon in response to environmental Pi. Under phosphate limitation conditions the inner membrane sensor kinase PhoR autophosphorylates; Subsequent transfer of the phosphate group to PhoB results in activation of PhoB. It has been shown that PhoBR and PhoB are responsible for upregulation of phosphonate and glycerol phosphate metabolism and the high-affinity phosphate transport system, respectively, thus showing the complex regulation by the PhoR-PhoB two-component regulatory system.PMID: 2693738 PMID: 18031348 PMID: 23138451Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicN/AyesyesNo330.201CYT0-0.200913-00NiCytoplasmic9
FECI_ECOLIP23484
fecI
b4293 JW4253 cytoplasmic nucleoid peripheral IM protein, Probable RNA polymerase sigma factor FecI Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Interacts with FecR at the inner membrane propably through the N-terminal region of FecR (1-85) which is cytoplasmicPMID: 16718597. FecR spans the cytoplasmic membrane once, interacts in the periplasm with its C-terminal region with FecA occupied by ferric citrate, and transmits the information through the cytoplasmic membrane into the cytoplasm, where it converts FecI into an active sigma factorPMID: 9573190. FecI specifically binds FecR (a transmembrane protein) and that this binding is necessary for FecI to function as a sigma factorPMID: 12057967. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 12057967 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AnoyesNo230.11CYT0-0.200913-00NiCytoplasmic1016
RRAA_ECOLIP0A8R0
rraA

menG yiiV

menG yiiVb3929 JW3900 cytoplasmic nucleoid peripheral IM protein, Regulator of ribonuclease activity A RraACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Negative regulator of RNaseE. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AnoyesNo340.108CYT0-0.200913-00NiCytoplasmic9
RRAB_ECOLIP0AF90
rraB

yjgD

yjgDb4255 JW4212 cytoplasmic nucleoid peripheral IM protein, Regulator of ribonuclease activity B RraBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Negative regulator of RNaseE. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AyesyesNo410.107CYT0-0.200913-00NiCytoplasmic8
DNAB_ECOLIP0ACB0
dnaB

groP grpA

groP grpAb4052 JW4012 cytoplasmic nucleoid peripheral IM protein, Replicative DNA helicase DnaBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/ATFyesyesNo30.105CYT0-0.200913-00NiCytoplasmic981
RNC_ECOLIP0A7Y0
rnc
b2567 JW2551 cytoplasmic nucleoid peripheral IM protein, Ribonuclease 3 RncCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 4865702 PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/ATFyesyesNo560.133CYT0-0.200913-00NiCytoplasmic10116
RPOD_ECOLIP00579
rpoD

alt

altb3067 JW3039 cytoplasmic nucleoid peripheral IM protein, RNA polymerase sigma factor RpoD Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/ATFyesyesNo260.104CYT0-0.200913-00NiCytoplasmic992
RPOS_ECOLIP13445
rpoS

appR katF nur otsX sigS

appR katF nur otsX sigSb2741 JW5437 cytoplasmic nucleoid peripheral IM protein, RNA polymerase sigma factor RpoS Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AnoyesNo690.1CYT0-0.200913-00NiCytoplasmic1030
RPPH_ECOLIP0A776
rppH

nudH ygdP

nudH ygdPb2830 JW2798 cytoplasmic nucleoid peripheral IM protein, RNA pyrophosphohydrolase RppHCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AyesyesNo250.126CYT0-0.200913-00NiCytoplasmic962
HFQ_ECOLIP0A6X3
hfq
b4172 JW4130 cytoplasmic nucleoid peripheral IM protein, RNA-binding HfqCytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Nucleoid (Experimental)The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain. Is a bacterial protein involved in several aspects of nucleic acid transactions. Nucleoid-associated protein (NAP) that binds sequence-nonspecific DNA. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 20011543 PMID: 21541338 PMID: 24438330Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AyesyesNo530.107CYT0-0.200913-00NiCytoplasmic1075
NUSA_ECOLIP0AFF6
nusA
b3169 JW3138 cytoplasmic nucleoid peripheral IM protein, Transcription termination/antitermination NusA Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of NusA have been monitored via fluorescence microscopy. NusA was found to be located predominantly in two clusters associated with each nucleoid. NusA can self-assemble into oligomers under heat shock conditions. NusA also acquires chaperone activity.PMID: 17272300 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AyesyesNo410.104CYT0-0.200913-00NiCytoplasmic999
CPXR_ECOLIP0AE88
cpxR

yiiA

yiiAb3912 JW3883 cytoplasmic nucleoid peripheral IM protein, transcriptional regulator CpxRCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 8294007 PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cytoplasm CytoplasmicN/AyesyesNo320.14CYT0-0.200913-00NiCytoplasmic10118
OMPR_ECOLIP0AA16
ompR

kmt ompB

kmt ompBb3405 JW3368 cytoplasmic nucleoid peripheral IM protein, transcriptional regulator OmpRCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo380.124CYT0-0.200913-00NiCytoplasmic1087
RCSB_ECOLIP0DMC7
rcsB
b2217 JW2205 cytoplasmic nucleoid peripheral IM protein, transcriptional regulator RcsBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Regulator protein RcsB is a member of the two-component regulatory system RcsC/RcsB. It is involved in the activation of colanic acid capsule synthesis (cps) and cell division (ftsZ) genes. It is believed that the RcsB protein is activated through phosphorylation, probably by the sensor protein RcsC. RcsB protein for Regulator capsule synthesis B, is a response regulator that belongs to the multicomponent RcsF/RcsC/RcsD/RcsA-RcsB phosphorelay system. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). PMID: 2404948 Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1CytoplasmicN/AnoyesNo660.117CYT0-0.200913-00NiCytoplasmic1090
NADR_ECOLIP27278
nadR

nadI

nadIb4390 JW5800 cytoplasmic nucleoid peripheral IM protein, Trifunctional NAD biosynthesis/regulator NadR Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)May loosely associate with the membrane through its interaction with the membrane-bound pnuC (Nicotinamide riboside transporter). In combination with corepressor (NAD?), the cytoplasmic form of nadR would be capable of acting as a transcriptional repressor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 10464228 PMID: 23138451 PMID: 23230279Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1A,N,F1Cell membrane; Peripheral membrane protein Cytoplasm CytoplasmicN/AyesyesNo390.131CYT0-0.200913-00NiCytoplasmic839
AMPH_ECOLIP0AD70
ampH

yaiH

yaiHb0376 JW5052 cytoplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein PMID: 25664544PMID: 22001512Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane PeriplasmicN/ASurAyesyesYes220.613SpI2116.801-00Yn5-16c21/22oPeriplasmic941
AEEP_ECOLIP51981
ycjG

ycjH

ycjHb1325 JW1318 cytoplasmic peripheral IM protein (SEC through IM), L-Ala-D/L-Glu epimerase YcjGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo210.226CYT0-0.200913-00NiCytoplasmic819
ENO_ECOLIP0A6P9
eno
b2779 JW2750 cytoplasmic peripheral IM protein facing the cytoplasm & peripheral OM protein facing the extra-cellular space (SEC through IM), Enolase EnoCytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Probable)When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. PMID: 8610017 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 17242352 PMID: 8610017 PMID: 15003462 PMID: 18337249 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceA, F1, F4A,F1,F4Cytoplasm; cytoskeleton Secreted Cell surface Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionSECyesyesNo420.139CYT0-0.200913-00NiCytoplasmic9100
DNAK_ECOLIP0A6Y8
dnaK

groP grpF seg

groP grpF segb0014 JW0013 cytoplasmic peripheral IM protein of Hsp70 family, Chaperone DnaKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)DnaK associates with the inner membrane. The ability to isolate DnaK with a membrane fraction prepared under gentle lysis conditions supports a peripheral association between DnaK and the cytoplasmic membrane. PMID: 7961473 Overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to green fluorescent protein (GFP) led to an increased association (2.3???4.8-fold) of DnaK with the membrane. PMID: 17446557 PMID: 1396676 PMID: 7783627 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 16079137 PMID: 17446557 PMID: 1396676 PMID: 7783627 PMID: 23230279 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo200.127CYT0-0.200913-00NiCytoplasmic10115
LOLD_ECOLIP75957
lolD

ycfV

ycfVb1117 JW5162 cytoplasmic peripheral IM protein, -releasing system ATP-binding LolD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo650.122CYT0-0.200913-00NiCytoplasmicMembrane1025
PAAC_ECOLIP76079
paaC

ydbP

ydbPb1390 JW1385 cytoplasmic peripheral IM protein, 1,2-phenylacetyl-CoA epoxidase, subunit C PaaCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo570.144CYT0-0.200913-00NiCytoplasmic8
DXS_ECOLIP77488
dxs

yajP

yajPb0420 JW0410 cytoplasmic peripheral IM protein, 1-deoxy-D-xylulose-5-phosphate synthase DxsCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300PMID: 23230279 PMID: 17272300Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo520.114CYT0-0.200913-00NiCytoplasmic921
CH10_ECOLIP0A6F9
groS

groES mopB

groES mopBb4142 JW4102 cytoplasmic peripheral IM protein, 10 kDa chaperonin GroSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 22380631 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo430.185CYT0-0.200913-00NiCytoplasmic1086
DAPD_ECOLIP0A9D8
dapD
b0166 JW0161 cytoplasmic peripheral IM protein, 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase DapDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo240.195CYT0-0.200913-00NiCytoplasmic1041
GPMA_ECOLIP62707
gpmA

gpm pgm pgmA

gpm pgm pgmAb0755 JW0738 cytoplasmic peripheral IM protein, 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase GpmACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo410.153CYT0-0.200913-00NiCytoplasmic8102
KBL_ECOLIP0AB77
kbl
b3617 JW3592 cytoplasmic peripheral IM protein, 2-amino-3-ketobutyrate coenzyme A ligase KblCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo340.12CYT0-0.200913-00NiCytoplasmic937
RIDA_ECOLIP0AF93
ridA

yjgF

yjgFb4243 JW5755 cytoplasmic peripheral IM protein, 2-iminobutanoate/2-iminopropanoate deaminase RidACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo210.115CYT0-0.200913-00NiCytoplasmic975
UBIH_ECOLIP25534
ubiH

visB

visBb2907 JW2875 cytoplasmic peripheral IM protein, 2-octaprenyl-6-methoxyphenol hydroxylase UbiHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)The biosynthesis of UQ is studied in great detail in bacteria and yeast but only to a limited extent in animal tissues. The UQ biosynthetic enzymes may constitute a complex that is tightly bound to the membrane.PMID: 11583838Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo420.38SpI251.25365-00Yn3-13c18/19oUnknown615
ODO1_ECOLIP0AFG3
sucA
b0726 JW0715 cytoplasmic peripheral IM protein, 2-oxoglutarate dehydrogenase E1 component SucACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279 PMID: 15919657Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo240.133CYT0-0.200913-00NiCytoplasmic96
MENE_ECOLIP37353
menE
b2260 JW2255 cytoplasmic peripheral IM protein, 2-succinylbenzoate--CoA ligase MenECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homologous to FadD13 protein of Mycobacterium that is peripheral.PMID: 22560731Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo480.113CYT0-0.200913-00NiCytoplasmic931
RLUF_ECOLIP32684
rluF

yjbC

yjbCb4022 JW3982 cytoplasmic peripheral IM protein, 23S rRNA pseudouridine(2604) synthase RluFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo250.109CYT0-0.200913-00NiCytoplasmic9
CYSQ_ECOLIP22255
cysQ

amtA

amtAb4214 JW4172 cytoplasmic peripheral IM protein, 3'(2'),5'-bisphosphate nucleotidase CysQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 15911532PMID: 15911532Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Membrane associated (Experimental)N/AnoyesNo400.152CYT0-0.200913-00NiCytoplasmic983
RIBB_ECOLIP0A7J0
ribB

htrP

htrPb3041 JW3009 cytoplasmic peripheral IM protein, 3,4-dihydroxy-2-butanone 4-phosphate synthase RibBCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicN/AyesyesNo220.191CYT0-0.200913-00NiCytoplasmic9116
KDSC_ECOLIP0ABZ4
kdsC

yrbI yrbJ

yrbI yrbJb3198 JW3165 cytoplasmic peripheral IM protein, 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Short signal peptide of 17aa. Prediction by Phobius and the SignalP NN approach/extensive islands of hydrophobicity by K/D. No obvious biochemical reason that the enzyme should be secreted. Signal peptide like sequence could act as peripheral membrane association element. Lipopolysaccharide (LPS) is an amphiphilic molecule composed of a hydrophilic heteropolysaccharide and the membrane-embedded Lipid A. The innermost 8-carbon sugar 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) is the only conserved structural element found in all LPS, linking the lipid A to the carbohydrate domain of the molecule. The KDO 8-phosphate phosphatase (KdsC) is one of the enzymes of the KDO biosynthesis pathway and is responsible for cleavage of the phosphate group from KDO 8-phosphate to yield KDO. PMID: 12639950 In Escherichia coli, KdsC has been characterized as a homotetrameric molecule with a random-coiled C-terminal tail in each subunit. PMID: 12639950 PMID: 12639950PMID: 12639950Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)N/AyesyesNo180.326CYT0-0.200913-00Yn4-12c17/18oCytoplasmic990
FABZ_ECOLIP0A6Q6
fabZ

sefA yaeA

sefA yaeAb0180 JW0175 cytoplasmic peripheral IM protein, 3-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 8354462 PMID: 7806516 PMID: 8805534 PMID: 8910376 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15911532 PMID: 8910376 PMID: 8354462 PMID: 7806516 PMID: 8805534 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)N/ATFyesyesNo320.104CYT0-0.200913-00NiCytoplasmic1027
FADI_ECOLIP76503
fadI

yfcY

yfcYb2342 JW2339 cytoplasmic peripheral IM protein, 3-ketoacyl-CoA thiolase FadICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo320.192CYT0-0.200913-00NiCytoplasmic99
UBID_ECOLIP0AAB4
ubiD

yigC yigY

yigC yigYb3843 JW3819 cytoplasmic peripheral IM protein, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicIM (predicted)yesyesNo650.107CYT0-0.200913-00NiCytoplasmic937
FABG_ECOLIP0AEK2
fabG
b1093 JW1079 cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) reductase FabGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo230.249CYT0-0.200913-00NiCytoplasmic993
FABB_ECOLIP0A953
fabB

fabC

fabCb2323 JW2320 cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) synthase 1 FabBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo250.163CYT0-0.200913-00Yn4-15c24/25oCytoplasmic1013
FABF_ECOLIP0AAI5
fabF

fabJ

fabJb1095 JW1081 cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) synthase 2 FabFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo480.158CYT0-0.200913-00Yn6-14c20/21oCytoplasmic98
HSLO_ECOLIP0A6Y5
hslO

yrfI

yrfIb3401 JW5692 cytoplasmic peripheral IM protein, 33 kDa chaperonin HslOCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (230 232;263 266) PMID: 10764757PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicIM (predicted)yesyesNo630.125CYT0-0.200913-00NiCytoplasmic1088
MALQ_ECOLIP15977
malQ

malA

malAb3416 JW3379 cytoplasmic peripheral IM protein, 4-alpha-glucanotransferase MalQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo230.214SpI220.437923-00NiCytoplasmic1031
GABT_ECOLIP22256
gabT
b2662 JW2637 cytoplasmic peripheral IM protein, 4-aminobutyrate aminotransferase GabTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo650.16CYT0-0.200913-00NiCytoplasmic977
ISPG_ECOLIP62620
ispG

gcpE

gcpEb2515 JW2499 cytoplasmic peripheral IM protein, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase IspGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo440.118CYT0-0.200913-00NiCytoplasmic9
RISB_ECOLIP61714
ribE

ribH ybaF

ribH ybaFb0415 JW0405 cytoplasmic peripheral IM protein, 6,7-dimethyl-8-ribityllumazine synthase RibECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo690.129CYT0-0.200913-00NiCytoplasmic9
6PGD_ECOLIP00350
gnd
b2029 JW2011 cytoplasmic peripheral IM protein, 6-phosphogluconate dehydrogenase, decarboxylating GndCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo200.182CYT0-0.200913-00NiCytoplasmic890
CH60_ECOLIP0A6F5
groL

groEL mopA

groEL mopAb4143 JW4103 cytoplasmic peripheral IM protein, 60 kDa chaperonin GroLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo580.12CYT0-0.200913-00NiCytoplasmic1094
MODF_ECOLIP31060
modF

phrA

phrAb0760 JW0743 cytoplasmic peripheral IM protein, ABC transporter ATP-binding ModFCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AnoyesNo340.221CYT0-0.200913-00NiCytoplasmicMembrane933
UUP_ECOLIP43672
uup

ycbH ycbI

ycbH ycbIb0949 JW0932 cytoplasmic peripheral IM protein, ABC transporter ATP-binding UupCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.126CYT0-0.200913-00NiCytoplasmic10
YADG_ECOLIP36879
yadG
b0127 JW0123 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YadGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo310.246CYT0-0.200913-00NiCytoplasmicMembrane75
YBBA_ECOLIP0A9T8
ybbA
b0495 JW0484 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YbbACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalKCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo410.105CYT0-0.200913-00NiCytoplasmicMembrane10102
YBIT_ECOLIP0A9U3
ybiT
b0820 JW0804 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YbiTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo190.108CYT0-0.200913-00NiCytoplasmic942
YDCT_ECOLIP77795
ydcT
b1441 JW1436 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YdcTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo200.146CYT0-0.200913-00NiCytoplasmicMembrane1026
YEJF_ECOLIP33916
yejF
b2180 JW2168 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YejFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo350.139CYT0-0.200913-00NiCytoplasmicMembrane9
YHES_ECOLIP63389
yheS
b3352 JW3315 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YheSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding proteins Trigger factor-sensitive aggregation.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo540.115CYT0-0.200913-00NiCytoplasmic939
YNJD_ECOLIP76909
ynjD
b1756 JW5286 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YnjDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo530.159CYT0-0.200913-00NiCytoplasmicMembrane919
YPHE_ECOLIP77509
yphE
b2547 JW2531 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YphECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo260.136CYT0-0.200913-00NiCytoplasmicMembrane75
YTFR_ECOLIQ6BEX0
ytfR

ytfS

ytfSb4485 JW5752 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YtfRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/Apseudogene (Ochman & Davalos, 2006)noyesNo250.128CYT0-0.200913-00NiCytoplasmicMembrane1024
ACKA_ECOLIP0A6A3
ackA

ack

ackb2296 JW2293 cytoplasmic peripheral IM protein, Acetate kinase AckACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo200.328CYT0-0.200913-00NiCytoplasmic10112
ACCA_ECOLIP0ABD5
accA
b0185 JW0180 cytoplasmic peripheral IM protein, Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha AccACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.103CYT0-0.200913-00NiCytoplasmic1028
ACCD_ECOLIP0A9Q5
accD

dedB usg

dedB usgb2316 JW2313 cytoplasmic peripheral IM protein, Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta AccDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation related.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo180.112CYT0-0.200913-00NiCytoplasmic962
ACSA_ECOLIP27550
acs

yfaC

yfaCb4069 JW4030 cytoplasmic peripheral IM protein, Acetyl-coenzyme A synthetase AcsCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous to FadD13 protein of Mycobacterium that is peripheral. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 22560731 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo270.136CYT0-0.200913-00NiCytoplasmic962
ARGB_ECOLIP0A6C8
argB
b3959 JW5553 cytoplasmic peripheral IM protein, Acetylglutamate kinase ArgBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo220.115CYT0-0.200913-00NiCytoplasmic981
ARGE_ECOLIP23908
argE
b3957 JW3929 cytoplasmic peripheral IM protein, Acetylornithine deacetylase ArgECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo230.163CYT0-0.200913-00NiCytoplasmic942
ACNB_ECOLIP36683
acnB

yacI yacJ

yacI yacJb0118 JW0114 cytoplasmic peripheral IM protein, Aconitate hydratase B AcnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 10585860 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10585860Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)N/ATFyesyesNo420.124CYT0-0.200913-00NiCytoplasmic990
ACP_ECOLIP0A6A8
acpP
b1094 JW1080 cytoplasmic peripheral IM protein, Acyl carrier AcpPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of phospholipid metabolism complex PlsB-PssA-YbgC-AcpPMID: 16294310. Various methods were used to demonstrate that ACP could be cross-linked specifically to an inner membrane protein of E. coli, most probably to the glycerol-3-phosphate acyl transferase (GPAT)PMID: 1310877. Evidence for interactions of acyl carrier protein with glycerol-3-phosphate acyltransferase, an inner membrane protein of Escherichia coliPMID: 2676605. Membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier proteinPMID: 2668033. PMID: 3884600 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1310877 PMID: 2676605 PMID: 2668033 PMID: 3884600 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)N/Apseudogene (Ochman & Davalos, 2006)noyesNo470.119CYT0-0.200913-00NiCytoplasmic10
YBGC_ECOLIP0A8Z3
ybgC
b0736 JW0726 cytoplasmic peripheral IM protein, Acyl-CoA thioester hydrolase YbgCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Phospholipid metabolism complex PlsB-PssA-YbgC-acp PMID: 16294310Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo520.116CYT0-0.200913-00NiCytoplasmic970
ADD_ECOLIP22333
add
b1623 JW1615 cytoplasmic peripheral IM protein, Adenosine deaminase AddCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo400.118CYT0-0.200913-00NiCytoplasmic915
KAD_ECOLIP69441
adk

dnaW plsA

dnaW plsAb0474 JW0463 cytoplasmic peripheral IM protein, Adenylate kinase AdkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Adk cell localization has been monitored via fluorescence microscopy after protein was chromosomally tagged with fluorescent protein/s. These experiments indicate that Adk is distributed diffusely throughout cytoplasm. PMID: 8650159PMID: 17272300 PMID: 23230279 PMID: 8650159Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo180.12CYT0-0.200913-00Yn3-12c17/18oCytoplasmic10104
PUR8_ECOLIP0AB89
purB
b1131 JW1117 cytoplasmic peripheral IM protein, Adenylosuccinate lyase PurBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo610.215CYT0-0.200913-00NiCytoplasmic974
PURA_ECOLIP0A7D4
purA

adeK

adeKb4177 JW4135 cytoplasmic peripheral IM protein, Adenylosuccinate synthetase PurACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo620.102CYT0-0.200913-00NiCytoplasmic1041
RFAF_ECOLIP37692
rfaF

waaF

waaFb3620 JW3595 cytoplasmic peripheral IM protein, ADP-heptose--LPS heptosyltransferase 2 RfaFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo420.134CYT0-0.200913-00NiCytoplasmic924
HLDD_ECOLIP67910
hldD

htrM rfaD waaD

htrM rfaD waaDb3619 JW3594 cytoplasmic peripheral IM protein, ADP-L-glycero-D-manno-heptose-6-epimerase HldDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 2198271Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)N/AyesyesNo190.195CYT0-0.200913-00NiCytoplasmic926
ADPP_ECOLIQ93K97
nudF

aspP yqiE yzzG

aspP yqiE yzzGb3034 JW3002 cytoplasmic peripheral IM protein, ADP-ribose pyrophosphatase NudFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble IM Fraction +yesyesNo380.101CYT0-0.200913-00NiCytoplasmic857
GLPD_ECOLIP13035
glpD

glyD

glyDb3426 JW3389 cytoplasmic peripheral IM protein, Aerobic glycerol-3-phosphate dehydrogenase GlpDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Binds to the cytoplasmic membrane through an amphipathic C-terminal alpha-helix (357-374)PMID: 11955283. Identified in PlsB-PflB-GlpD-Psd PMID: 20521846. Detergent-depleted enzyme can be functionally reconstituted with Escherichia coli membrane vesicles to support glycerol-3-phosphate-dependent active transport of L-prolinePMID: 340460. When GlpD is lost by mutation, the cells not only fail to grow on glycerol or l-alpha-glycerophosphate, but are subject to growth inhibition in the presence of either compound PMID: 5321485. Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. PMID: 1987111 GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. PMID: 6387427 Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8982270 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 11955283 PMID: 340460 PMID: 15919657 PMID: 15911532 PMID: 8982270 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo350.138CYT0-0.200913-00NiCytoplasmic1048
ALR2_ECOLIP29012
dadX

alnB dadB

alnB dadBb1190 JW1179 cytoplasmic peripheral IM protein, Alanine racemase, catabolic DadXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo250.206CYT0-0.200913-00NiCytoplasmic102
YQHD_ECOLIQ46856
yqhD
b3011 JW2978 cytoplasmic peripheral IM protein, Alcohol dehydrogenase YqhDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo210.138CYT0-0.200913-00NiCytoplasmic991
ADHP_ECOLIP39451
adhP

yddN

yddNb1478 JW1474 cytoplasmic peripheral IM protein, Alcohol dehydrogenase, propanol-preferring AdhPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo400.124CYT0-0.200913-00NiCytoplasmic986
YAHK_ECOLIP75691
yahK
b0325 JW0317 cytoplasmic peripheral IM protein, Aldehyde reductase YahKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)noyesNo530.148CYT0-0.200913-00NiCytoplasmic973
ADHE_ECOLIP0A9Q7
adhE

ana

anab1241 JW1228 cytoplasmic peripheral IM protein, Aldehyde-alcohol dehydrogenase AdhECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo560.127CYT0-0.200913-00NiCytoplasmic945
SSUB_ECOLIP0AAI1
ssuB

ycbE

ycbEb0933 JW0916 cytoplasmic peripheral IM protein, Aliphatic sulfonates import ATP-binding SsuBCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo220.181CYT0-0.200913-00NiCytoplasmicMembrane1023
AHPC_ECOLIP0AE08
ahpC
b0605 JW0598 cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase C AhpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (47 47;166 166) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo490.111CYT0-0.200913-00NiCytoplasmic1090
AHPF_ECOLIP35340
ahpF
b0606 JW0599 cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase subunit F AhpFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (345 348) PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo350.108CYT0-0.200913-00NiCytoplasmicMembrane776
PHNL_ECOLIP16679
phnL
b4096 JW4057 cytoplasmic peripheral IM protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo340.263CYT0-0.200913-00NiCytoplasmicMembrane98
UXAA_ECOLIP42604
uxaA

ygjW

ygjWb3091 JW3062 cytoplasmic peripheral IM protein, Altronate dehydratase UxaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo680.185CYT0-0.200913-00NiCytoplasmic8113
PUR1_ECOLIP0AG16
purF
b2312 JW2309 cytoplasmic peripheral IM protein, Amidophosphoribosyltransferase PurFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo320.119CYT0-0.200913-00NiCytoplasmic915
YHDZ_ECOLIP45769
yhdZ
b3271 JW3239 cytoplasmic peripheral IM protein, amino-acid ABC transporter ATP-binding YhdZCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo120.122CYT0-0.200913-00NiCytoplasmicMembrane911
GCST_ECOLIP27248
gcvT
b2905 JW2873 cytoplasmic peripheral IM protein, Aminomethyltransferase GcvTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo310.116CYT0-0.200913-00NiCytoplasmic9101
AMPN_ECOLIP04825
pepN
b0932 JW0915 cytoplasmic peripheral IM protein, Aminopeptidase PepNCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous peptidases are membrane associated in mammals, some data propose that the enzyme may associate with the E.coli membrane. It is structurally classified with <a href="http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/enzymes/GetPage.pl?ec_number=3.4.11.2">EC 3.4.11.2</a> membrane alanyl aminopeptidases. However, the extent of membrane association needs further analysis as it is generally soluble in the cytoplasmic fraction of E.coli and other bacteria. PMID: 323010 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 323010 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmic (Experimental)N/AyesyesNo500.108CYT0-0.200913-00NiCytoplasmicMembrane10101
GLPA_ECOLIP0A9C0
glpA
b2241 JW2235 cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit A GlpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo620.155CYT0-0.200913-00NiCytoplasmicMembrane105
GLPB_ECOLIP13033
glpB
b2242 JW2236 cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit B GlpBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. PMID: 6387427 Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. PMID: 1987111 PMCID: PMC3210124 PMID: 3286606PMID: 20706981 PMID: 6387427 PMID: 1987111 PMCID: PMC3210124 PMID: 3286606Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein Cytoplasmic (Experimental)IM (predicted)yesyesNo300.137CYT0-0.200913-00Yn10-21c28/29oCytoplasmicMembrane1024
GLPC_ECOLIP0A996
glpC
b2243 JW2237 cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit C GlpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. PMID: 3286606 PMID: 3286606Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein Membrane associated (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo350.222CYT0-0.200913-00NiCytoplasmicMembrane1013
TRPE_ECOLIP00895
trpE
b1264 JW1256 cytoplasmic peripheral IM protein, Anthranilate synthase component 1 TrpECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo190.11CYT0-0.200913-00NiCytoplasmic1066
ARAG_ECOLIP0AAF3
araG
b1900 JW1888 cytoplasmic peripheral IM protein, Arabinose import ATP-binding AraGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 7028715Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo380.293CYT0-0.200913-00NiCytoplasmicMembrane1037
ARTP_ECOLIP0AAF6
artP
b0864 JW0848 cytoplasmic peripheral IM protein, Arginine transport ATP-binding ArtPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) The artPIQM artJ genes encode a third arginine-uptake system in addition to the known argT hisJQMP system of Salmonella typhimurium and E. coli and the arginine (-ornithine) carrier (aps) of E. coli. The artQ, artM and artP products are similar to the transmembraneous proteins and the ATPase of binding-protein-dependent carriers. PMID: 8801422 PMID: 8801422 PMID: 15758229 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 8801422 PMID: 15758229 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo200.124CYT0-0.200913-00NiCytoplasmicMembrane726
ARLY_ECOLIP11447
argH
b3960 JW3932 cytoplasmic peripheral IM protein, Argininosuccinate lyase ArgHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo530.138CYT0-0.200913-00NiCytoplasmic9
ASSY_ECOLIP0A6E4
argG
b3172 JW3140 cytoplasmic peripheral IM protein, Argininosuccinate synthase ArgGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo370.163CYT0-0.200913-00NiCytoplasmic919
ULAC_ECOLIP69820
ulaC

ptxA sgaA yjfU

ptxA sgaA yjfUb4195 JW4153 cytoplasmic peripheral IM protein, Ascorbate-specific PTS system EIIA component UlaCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)PMID: 12644495Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo470.126CYT0-0.200913-00NiCytoplasmicMembrane784
ULAB_ECOLIP69822
ulaB

sgaB yjfT

sgaB yjfTb4194 JW4152 cytoplasmic peripheral IM protein, Ascorbate-specific PTS system EIIB component UlaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectivelyPMID: 12644495. All three proteins are required for the anaerobic utilization and uptake of L-ascorbate in vivo and its phosphoenolpyruvate-dependent phosphorylation in vitroPMID: 12644495.PMID: 12644495Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo620.14CYT0-0.200913-00NiUnknown253
ASNB_ECOLIP22106
asnB
b0674 JW0660 cytoplasmic peripheral IM protein, Asparagine synthetase B AsnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo370.141CYT0-0.200913-00NiCytoplasmic9
SYN_ECOLIP0A8M0
asnS

tss

tssb0930 JW0913 cytoplasmic peripheral IM protein, Asparagine--tRNA ligase AsnSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/ATFyesyesNo530.182CYT0-0.200913-00NiCytoplasmic1070
AAT_ECOLIP00509
aspC
b0928 JW0911 cytoplasmic peripheral IM protein, Aspartate aminotransferase AspCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 3521591Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)N/AyesyesNo230.126CYT0-0.200913-00NiCytoplasmic10
ASPA_ECOLIP0AC38
aspA
b4139 JW4099 cytoplasmic peripheral IM protein, Aspartate ammonia-lyase AspACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected with chromosomally tap-tagged TF with >80% confidence score PMID: 15690043PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo220.123CYT0-0.200913-00NiCytoplasmic987
SYD_ECOLIP21889
aspS

tls

tlsb1866 JW1855 cytoplasmic peripheral IM protein, Aspartate--tRNA ligase AspSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SecY-interacting protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19139097 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo270.104CYT0-0.200913-00NiCytoplasmic1081
DHAS_ECOLIP0A9Q9
asd

hom

homb3433 JW3396 cytoplasmic peripheral IM protein, Aspartate-semialdehyde dehydrogenase AsdCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo440.197CYT0-0.200913-00NiCytoplasmic918
HIS1_ECOLIP60757
hisG
b2019 JW2001 cytoplasmic peripheral IM protein, ATP phosphoribosyltransferase HisGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo440.181CYT0-0.200913-00NiCytoplasmic1064
ATPE_ECOLIP0A6E6
atpC

papG uncC

papG uncCb3731 JW3709 cytoplasmic peripheral IM protein, ATP synthase epsilon chain AtpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 16079137 PMID: 21778229 PMID: 9868784 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionnoyesNo410.107CYT0-0.200913-00NiCytoplasmicMembrane10120
ATPG_ECOLIP0ABA6
atpG

papC uncG

papC uncGb3733 JW3711 cytoplasmic peripheral IM protein, ATP synthase gamma chain AtpGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 7508444 PMID: 2900236 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 7508444 PMID: 2900236 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo410.162CYT0-0.200913-00NiCytoplasmicMembrane1048
ATPA_ECOLIP0ABB0
atpA

papA uncA

papA uncAb3734 JW3712 cytoplasmic peripheral IM protein, ATP synthase subunit alpha AtpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicIM (predicted)TFnoyesNo280.106CYT0-0.200913-00NiCytoplasmic1060
ATPB_ECOLIP0ABB4
atpD

papB uncD

papB uncDb3732 JW3710 cytoplasmic peripheral IM protein, ATP synthase subunit beta AtpDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo230.123CYT0-0.200913-00NiCytoplasmicMembrane1077
ATPD_ECOLIP0ABA4
atpH

papE uncH

papE uncHb3735 JW3713 cytoplasmic peripheral IM protein, ATP synthase subunit delta AtpHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo360.135CYT0-0.200913-00NiCytoplasmic1089
PFKA_ECOLIP0A796
pfkA
b3916 JW3887 cytoplasmic peripheral IM protein, ATP-dependent 6-phosphofructokinase isozyme 1 PfkACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo200.426CYT0-0.200913-00NiCytoplasmic1076
HSLU_ECOLIP0A6H5
hslU

htpI

htpIb3931 JW3902 cytoplasmic peripheral IM protein, ATP-dependent protease ATPase subunit HslUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo240.141CYT0-0.200913-00NiCytoplasmic1074
DBPA_ECOLIP21693
dbpA
b1343 JW1337 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase DbpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; Fragment of 23S rRNA. Assembly cofactor of ribosome; Fragment of 23S rRNA. PMID: 21529161PMID: 21529161Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo320.341CYT0-0.200913-00NiCytoplasmic912
DEAD_ECOLIP0A9P6
deaD

csdA mssB rhlD

csdA mssB rhlDb3162 JW5531 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase DeaDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation PMID: 19737520. Contains a SPOR domain. PMID: 8552679PMID: 19880599 PMID: 23230279 PMID: 8552679Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo350.151CYT0-0.200913-00NiCytoplasmic1050
HRPA_ECOLIP43329
hrpA
b1413 JW5905 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase HrpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo520.107CYT0-0.200913-00NiCytoplasmic8
RHLE_ECOLIP25888
rhlE
b0797 JW0781 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase RhlECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associates with the 70S subunit. Overex<x>pression of protein suppresses defects in deletion of DeaD. PMID: 18083833PMID: 23230279 PMID: 18083833Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo460.14CYT0-0.200913-00NiCytoplasmic962
SRMB_ECOLIP21507
srmB

rbaB rhlA

rbaB rhlAb2576 JW2560 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase SrmBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associate with the 50S subunit.PMID: 23230279 PMID: 15919657Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo330.109CYT0-0.200913-00NiCytoplasmic1068
LSRA_ECOLIP77257
lsrA

ego ydeX

ego ydeXb1513 JW1506 cytoplasmic peripheral IM protein, Autoinducer 2 import ATP-binding LsrACytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AnoyesNo420.176CYT0-0.200913-00NiCytoplasmicMembrane1017
GRCA_ECOLIP68066
grcA

yfiD

yfiDb2579 JW2563 cytoplasmic peripheral IM protein, Autonomous glycyl radical cofactor GrcACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo300.139CYT0-0.200913-00NiCytoplasmic9
AZUC_ECOLIC1P605
azuC
b4663 JW1891.1 cytoplasmic peripheral IM protein, AzuCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Small protein detected experimentally. AzuC-SPA was most abundant in the inner membrane fraction (77%) indicating this protein is bound to the cytoplasmic side of the inner membrane.PMID: 19121005 PMID: 21778229Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane -N/AnoyesNo270.099CYT0-0.200913-00NiUnknown2
FTNB_ECOLIP0A9A2
ftnB

yecI

yecIb1902 JW1890 cytoplasmic peripheral IM protein, Bacterial non-heme ferritin-like protein FtnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo480.152CYT0-0.200913-00NiCytoplasmic1082
WBBI_ECOLIP37749
wbbI

yefG

yefGb2034 JW2019 cytoplasmic peripheral IM protein, Beta-1,6-galactofuranosyltransferase WbbI WbbICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo420.102CYT0-0.200913-00NiCytoplasmic814
BGLR_ECOLIP05804
uidA

gurA gusA

gurA gusAb1617 JW1609 cytoplasmic peripheral IM protein, Beta-glucuronidase UidACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo590.12CYT0-0.200913-00NiCytoplasmic846
AK1H_ECOLIP00561
thrA

thrA1 thrA2

thrA1 thrA2b0002 JW0001 cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 1 ThrACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 4562989 PMID: 354697 PMID: 7003595 PMID: 6777249PMID: 15911532 PMID: 4562989 PMID: 354697 PMID: 7003595 PMID: 6777249Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)N/ATFyesyesNo150.142CYT0-0.200913-00NiCytoplasmic8
AK2H_ECOLIP00562
metL

metM

metMb3940 JW3911 cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 2 MetLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo240.117CYT0-0.200913-00NiCytoplasmicMembrane9
GLMU_ECOLIP0ACC7
glmU

yieA

yieAb3730 JW3708 cytoplasmic peripheral IM protein, Bifunctional GlmUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo490.123CYT0-0.200913-00NiCytoplasmic1094
ARNA_ECOLIP77398
arnA

pmrI yfbG

pmrI yfbGb2255 JW2249 cytoplasmic peripheral IM protein, Bifunctional polymyxin resistance ArnACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo590.178CYT0-0.200913-00NiCytoplasmic93
TRPGD_ECOLIP00904
trpGD

trpD

trpDb1263 JW1255 cytoplasmic peripheral IM protein, Bifunctional TrpGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Could not obtain any evidence for membrane binding (F1) Peripheral IM Cytoplasmic side. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 4594441 PMID: 6283099PMID: 19766000 PMID: 15911532 PMID: 4594441 PMID: 6283099Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo190.166CYT0-0.200913-00NiCytoplasmic922
ACCC_ECOLIP24182
accC

fabG

fabGb3256 JW3224 cytoplasmic peripheral IM protein, Biotin carboxylase AccCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation PMID: 19737520.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo360.117CYT0-0.200913-00NiCytoplasmic929
ARCM_ECOLIP77624
yahI
b0323 JW0315 cytoplasmic peripheral IM protein, Carbamate kinase-like YahICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo200.12CYT0-0.200913-00NiCytoplasmic982
CARB_ECOLIP00968
carB

pyrA

pyrAb0033 JW0031 cytoplasmic peripheral IM protein, Carbamoyl-phosphate synthase large chain CarBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo240.228CYT0-0.200913-00Yn9-16c21/22oUnknown4
CARA_ECOLIP0A6F1
carA

pyrA

pyrAb0032 JW0030 cytoplasmic peripheral IM protein, Carbamoyl-phosphate synthase small chain CarACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo220.124CYT0-0.200913-00NiCytoplasmic960
CLSB_ECOLIP0AA84
clsB

ybhO

ybhOb0789 JW0772 cytoplasmic peripheral IM protein, Cardiolipin synthase B ClsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. PMID: 22988102PMID: 22988102Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicN/AyesyesNo660.136CYT0-0.200913-00NiCytoplasmicMembrane727
PSS_ECOLIP23830
pssA

pss

pssb2585 JW2569 cytoplasmic peripheral IM protein, CDP-diacylglycerol--serine O-phosphatidyltransferase PssACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid metabolism complex PlsB-PssA-YbgC-Acp. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cell inner membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmicN/ATFyesyesNo480.108CYT0-0.200913-00NiCytoplasmic1035
LIT_ECOLIP11072
lit
b1139 JW1125 cytoplasmic peripheral IM protein, Cell death peptidase LitSingle weak hydrophobic region. Could be a transmembrane domain but could also be a peripheral inner membrane protein.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicN/AProphage Integrase (EcoGene)nonoNo200.181CYT0-0.200913-01Ni61-81oCytoplasmic8
FTSE_ECOLIP0A9R7
ftsE
b3463 JW3428 cytoplasmic peripheral IM protein, Cell division ATP-binding FtsECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Is the putative ATP-binding protein component of an ATP-binding cassette (ABC) transporter. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. PMID: 10048040 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 10048040 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo320.118CYT0-0.200913-00NiCytoplasmicMembrane1011
FTSA_ECOLIP0ABH0
ftsA

divA

divAb0094 JW0092 cytoplasmic peripheral IM protein, Cell division FtsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)FtsA has a conserved C-terminal membrane targeting sequence (MTS) with extremely weak TmHMM and Phobius predictions. Deletion or point mutations in the MTS, inactivate FtsA function and inhibit cell division. Because FtsA binds to the tubulin-like FtsZ protein that forms the Z ring, it is thought that the MTS of FtsA is required, along with the transmembrane protein ZipA, to assemble the Z ring and anchor it to the cytoplasmic membrane. FtsA function in cell division depends on additive effects of membrane binding and self-interaction, and that the specific requirement of an amphipathic helix for tethering FtsA to the membrane can be bypassed. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18186792 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo250.12CYT0-0.200913-00NiCytoplasmic984
FTSZ_ECOLIP0A9A6
ftsZ

sfiB sulB

sfiB sulBb0095 JW0093 cytoplasmic peripheral IM protein, Cell division FtsZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. Assembles at midcell at the inner surface of the cytoplasmic membrane. SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. PMID: 8917533 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Over-expression of NlpI can interrupt the nucleoids division and the assembly of FtsZ at the septum, mediated by IbpA/IbpB, suggesting a role of the NlpI/IbpA/IbpB complex in the cell division. PMID: 25699035PMID: 1944597 PMID: 20497333 PMID: 8917533 PMID: 23230279 PMID: 25699035Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo260.12CYT0-0.200913-00NiCytoplasmicMembrane1079
MINE_ECOLIP0A734
minE
b1174 JW1163 cytoplasmic peripheral IM protein, Cell division topological specificity factor MinECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature. PMID: 21738659PMID: 20025670 PMID: 10096083 PMID: 21738659Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo180.123CYT0-0.200913-00NiCytoplasmic9101
ZAPA_ECOLIP0ADS2
zapA

ygfE

ygfEb2910 JW2878 cytoplasmic peripheral IM protein, Cell division ZapACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Binds to the FtsZ ring. ZapA interacts directly with itself, FtsN, FtsZ and FtsI. Peripheral inner membrane protein via binding to the FtsZ ring. ZapA orthologue, YgfE, binds FtsZ polymers and promotes FtsZ polymer bundling. PMID: 12368265PMID: 20487275 PMID: 20497333 PMID: 17428494 PMID: 12368265Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo670.152CYT0-0.200913-00NiUnknown297
ZAPB_ECOLIP0AF36
zapB

yiiU

yiiUb3928 JW3899 cytoplasmic peripheral IM protein, Cell division ZapBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 20487275 PMID: 18394147Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo190.122CYT0-0.200913-00NiUnknown281
ZAPD_ECOLIP36680
zapD

yacF

yacFb0102 JW0099 cytoplasmic peripheral IM protein, Cell division ZapDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity. 22505682PMID: 22505682 22505682Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo390.116CYT0-0.200913-00NiCytoplasmic880
MREB_ECOLIP0A9X4
mreB

envB rodY

envB rodYb3251 JW3220 cytoplasmic peripheral IM protein, Cell shape-determining MreBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Bacterial actin homologue; is required for cell shape maintenance in most non-spherical bacteria, where it assembles into helical structures just underneath the cytoplasmic membrane; binds to the bitopic inner membrane protein RodZ as monomer or multimer. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. PMID: 19737520 PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones. [PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones. PMID: 24057063 PMID: 17090951 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 20168300 PMID: 19078962 PMID: 21816350 PMID: 19008860 PMID: 16391237 PMID: 24057063 PMID: 17090951 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo410.127CYT0-0.200913-00NiCytoplasmic962
CLPB_ECOLIP63284
clpB

htpM

htpMb2592 JW2573 cytoplasmic peripheral IM protein, Chaperone ClpBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 8376377Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo220.119CYT0-0.200913-00NiCytoplasmic1075
DNAJ_ECOLIP08622
dnaJ

groP

groPb0015 JW0014 cytoplasmic peripheral IM protein, Chaperone DnaJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 23230279 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo390.105CYT0-0.200913-00NiCytoplasmic1052
HTPG_ECOLIP0A6Z3
htpG
b0473 JW0462 cytoplasmic peripheral IM protein, Chaperone HtpGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo430.189CYT0-0.200913-00NiCytoplasmic10109
YCDY_ECOLIP75915
ycdY
b1035 JW1018 cytoplasmic peripheral IM protein, Chaperone YcdYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo380.184CYT0-0.200913-00NiCytoplasmicMembrane989
CHEA_ECOLIP07363
cheA
b1888 JW1877 cytoplasmic peripheral IM protein, Chemotaxis CheACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicIM (predicted), Soluble IM Fraction +noyesNo170.108CYT0-0.200913-00NiCytoplasmic1088
CISY_ECOLIP0ABH7
gltA

gluT icdB

gluT icdBb0720 JW0710 cytoplasmic peripheral IM protein, Citrate synthase GltACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo430.113CYT0-0.200913-00NiCytoplasmic922
CAIC_ECOLIP31552
caiC

yaaM

yaaMb0037 JW0036 cytoplasmic peripheral IM protein, Crotonobetaine/carnitine--CoA ligase CaiCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to FadD13 protein of Mycobacterium that is peripheral.PMID: 22560731Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo180.105CYT0-0.200913-00NiCytoplasmic9
BGLG_ECOLIP11989
bglG

bglC

bglCb3723 JW3701 cytoplasmic peripheral IM protein, Cryptic beta-glucoside bgl operon antiterminator BglGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)BglG is a transcriptional antiterminator which controls the expression bgl operon. It has been detected at the membrane when it is expressed with BglF, when expressed as monocistron, the bglG transcripts were observed at the cell poles (unpublished data). In the absence of ??-glucosidase, BglG also assembles near membrane and forms a pro-form complex with BglF that maintains BglG inactive. In the presence of sugar, BglG associates with the proteins of the sugar phosphotransfer system (PTS) and localizes at the cell poles. BglG requires two distinct (de)phosphorylation events to gain activity.PMID: 22984181Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo380.103CYT0-0.200913-00NiCytoplasmic848
PYRG_ECOLIP0A7E5
pyrG
b2780 JW2751 cytoplasmic peripheral IM protein, CTP synthase PyrGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PyrG=CTP synthase or CtpS in some systems. CTP synthase forms filaments in Caulobacter crescentus that regulate cell curvature independently of its catalytic function. The morphogenic role of CtpS requires its functional interaction with the intermediate filament, crescentin (CreS). Trigger factor-sensitive aggregation. PMID: 19737520 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 20639870 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)TFyesyesNo270.24SpI261.52462-10NiCytoplasmic977
CFA_ECOLIP0A9H7
cfa

cdfA

cdfAb1661 JW1653 cytoplasmic peripheral IM protein, Cyclopropane-fatty-acyl-phospholipid synthase CfaCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo380.183CYT0-0.200913-00NiCytoplasmic1026
CSDA_ECOLIQ46925
csdA

ygdJ

ygdJb2810 JW2781 cytoplasmic peripheral IM protein, Cysteine desulfurase CsdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Assembly cofactor of ribosome; associate with the 50S subunit. Under cold-shock conditions CsdA participates as an alternative DEAD-box RNA helicase together with RNase E in a ???cold shock??? RNA degradosome (Prud???homme-Genereux et al., 2004). It is therefore possible that CsdA may associate with the inner membrane via RNAseE.PMID: 21529161 PMID: 15554978Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo590.194CYT0-0.200913-00NiCytoplasmic99
ISCS_ECOLIP0A6B7
iscS

nuvC yfhO yzzO

nuvC yfhO yzzOb2530 JW2514 cytoplasmic peripheral IM protein, Cysteine desulfurase IscSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo480.104CYT0-0.200913-00NiCytoplasmic937
SUFS_ECOLIP77444
sufS

csdB ynhB

csdB ynhBb1680 JW1670 cytoplasmic peripheral IM protein, Cysteine desulfurase SufSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS probably through direct interaction.PMID: 12941942Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo660.18CYT0-0.200913-00NiCytoplasmic1011
SUFE_ECOLIP76194
sufE

ynhA

ynhAb1679 JW1669 cytoplasmic peripheral IM protein, Cysteine desulfuration SufECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction .PMID: 12941942Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo660.103CYT0-0.200913-00NiCytoplasmic9104
CYSK_ECOLIP0ABK5
cysK

cysZ

cysZb2414 JW2407 cytoplasmic peripheral IM protein, Cysteine synthase A CysKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo310.116CYT0-0.200913-00NiCytoplasmic977
CCMA_ECOLIP33931
ccmA

yejW

yejWb2201 JW5366 cytoplasmic peripheral IM protein, Cytochrome c biogenesis ATP-binding export CcmACytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo290.16CYT0-0.200913-00NiCytoplasmicMembrane747
AMPA_ECOLIP68767
pepA

carP xerB

carP xerBb4260 JW4217 cytoplasmic peripheral IM protein, Cytosol aminopeptidase PepACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo350.112CYT0-0.200913-00NiCytoplasmic958
ALSA_ECOLIP32721
alsA

yjcW

yjcWb4087 JW4048 cytoplasmic peripheral IM protein, D-allose import ATP-binding AlsACytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo210.291CYT0-0.200913-00NiCytoplasmicMembrane1018
DADA_ECOLIP0A6J5
dadA

dadR

dadRb1189 JW1178 cytoplasmic peripheral IM protein, D-amino acid dehydrogenase DadACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 6102989 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo230.155TMH00.264511-00Yn3-11c16/17oCytoplasmicMembrane1012
GATY_ECOLIP0C8J6
gatY

yegF

yegFb2096 JW5343 cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo490.137CYT0-0.200913-00NiCytoplasmic911
GATZ_ECOLIP0C8J8
gatZ
b2095 JW2082 cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo330.318CYT0-0.200913-00NiCytoplasmic86
DEOC_ECOLIP0A6L0
deoC

dra thyR

dra thyRb4381 JW4344 cytoplasmic peripheral IM protein, Deoxyribose-phosphate aldolase DeoCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo220.136CYT0-0.200913-00NiCytoplasmic10115
YIHI_ECOLIP0A8H6
yihI
b3866 JW3837 cytoplasmic peripheral IM protein, Der GTPase-activating YihICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associates with Der.PMID: 21529161Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo430.101CYT0-0.200913-00NiUnknown2108
PYRD_ECOLIP0A7E1
pyrD
b0945 JW0928 cytoplasmic peripheral IM protein, Dihydroorotate dehydrogenase PyrDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo450.146CYT0-0.200913-00NiCytoplasmicMembrane1012
ILVD_ECOLIP05791
ilvD
b3771 JW5605 cytoplasmic peripheral IM protein, Dihydroxy-acid dehydratase IlvDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo540.166CYT0-0.200913-00NiCytoplasmic925
DLDH_ECOLIP0A9P0
lpdA

lpd

lpdb0116 JW0112 cytoplasmic peripheral IM protein, Dihydroyl dehydrogenase LpdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 6352260 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (45 50) Disulfide bond-containing protein PMID: 25664544PMID: 16079137 PMID: 6352260 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo230.176CYT0-0.200913-00NiCytoplasmic1079
ODP2_ECOLIP06959
aceF
b0115 JW0111 cytoplasmic peripheral IM protein, Dihydroyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex AceFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15911532 PMID: 24742683 PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFnoyesNo520.106CYT0-0.200913-00NiCytoplasmic1095
ODO2_ECOLIP0AFG6
sucB
b0727 JW0716 cytoplasmic peripheral IM protein, Dihydroyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex SucBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. PMID: 9298646PMID: 23230279 PMID: 15919657 PMID: 9298646Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo190.112CYT0-0.200913-00NiCytoplasmic9122
DPPD_ECOLIP0AAG0
dppD
b3541 JW3510 cytoplasmic peripheral IM protein, Dipeptide transport ATP-binding DppDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo660.286CYT0-0.200913-00NiCytoplasmicMembrane719
DPPF_ECOLIP37313
dppF

dppE

dppEb3540 JW3509 cytoplasmic peripheral IM protein, Dipeptide transport ATP-binding DppFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo530.133CYT0-0.200913-00NiCytoplasmicMembrane924
YGHU_ECOLIQ46845
yghU
b2989 JW5492 cytoplasmic peripheral IM protein, Disulfide-bond oxidoreductase YghUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. localization peripheral inner membrane protein possibly also integral inner membrane/phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Membrane anchoredN/AyesyesNo240.332CYT0-0.200913-10NiCytoplasmic949
LEPA_ECOLIP60785
lepA
b2569 JW2553 cytoplasmic peripheral IM protein, Elongation factor 4 LepACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)LepA has an amino-terminal domain of 112 residues that is homologous to a domain of similar size found in initiation factor 2, elongation factor Tu, and elongation factor G (IF2, EF-Tu, and EF-G). GTP-photoaffinity labeling and subcellular fractionation experiments indicate that LepA a membrane-bound GTP-binding protein PMID: 2999765. GTPase, EF4/LepA involved in translational elongation, EF4 is stored at the membrane of Escherichia coli cells and released into the cytoplasm upon conditions of high ionic strength or low temperature where EF4 binds to stalled ribosomes and remobilizes themPMID: 21300907.Trigger factor-sensitive aggregationPMID: 19737520. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 2999765 PMID: 21300907 PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo390.117CYT0-0.200913-00NiCytoplasmicMembrane1045
EFG_ECOLIP0A6M8
fusA

far fus

far fusb3340 JW3302 cytoplasmic peripheral IM protein, Elongation factor G FusACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation PMID: 7042386PMID: 23230279 PMID: 7042386Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)N/AyesyesNo390.11CYT0-0.200913-00NiCytoplasmic962
EFTS_ECOLIP0A6P1
tsf
b0170 JW0165 cytoplasmic peripheral IM protein, Elongation factor Ts TsfCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AnoyesNo590.109CYT0-0.200913-00NiCytoplasmic1087
EFTU1_ECOLIP0CE47
tufA
b3339 JW3301 cytoplasmic peripheral IM protein, Elongation factor Tu 1 TufACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. PMID: 7525272 PMID: 7021545 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451 Disulfide bond-containing protein PMID: 25664544PMID: 20133608 PMID: 2022614 PMID: 775340 PMID: 7525272 PMID: 7021545 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cell inner membrane; Peripheral membrane protein Cytoplasmic (Experimental)N/AyesyesNo470.197CYT0-0.200913-00NiCytoplasmic1099
EFTU2_ECOLIP0CE48
tufB
b3980 JW3943 cytoplasmic peripheral IM protein, Elongation factor Tu 2 TufBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 2022614 PMID: 775340 PMID: 23230279 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cell inner membrane; Peripheral membrane protein CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo470.197CYT0-0.200913-00NiCytoplasmic1087
ETTA_ECOLIP0A9W3
ettA

yjjK

yjjKb4391 JW4354 cytoplasmic peripheral IM protein, Energy-dependent translational throttle EttACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo430.116CYT0-0.200913-00NiCytoplasmic992
FABI_ECOLIP0AEK4
fabI

envM

envMb1288 JW1281 cytoplasmic peripheral IM protein, Enoyl-(acyl-carrier-protein) reductase (NADH) FabICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo280.245CYT0-0.200913-00NiCytoplasmicMembrane1048
ENTB_ECOLIP0ADI4
entB

entG

entGb0595 JW0587 cytoplasmic peripheral IM protein, Enterobactin synthase component B EntBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 10692387Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo260.118CYT0-0.200913-00NiCytoplasmic865
ENTE_ECOLIP10378
entE
b0594 JW0586 cytoplasmic peripheral IM protein, Enterobactin synthase component E EntECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous to FadD13 protein of Mycobacterium that is peripheral. Phobius predits transmembrane region around aa100. Binds AMP. Very high homology to eight other transport proteins: (FADK_ECOLI, CAIC_ECOLI, LCFA_ECOLI, ENTF_ECOLI, ACSA_ECOLI) Membrane Association of the Escherichia coli Enterobactin Synthase Proteins EntB/G, EntE, and EntFPMID: 10692387Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Membrane CytoplasmicN/AyesyesNo360.15CYT0-0.200913-01No86-105iCytoplasmic988
ENTF_ECOLIP11454
entF
b0586 JW0578 cytoplasmic peripheral IM protein, Enterobactin synthase component F EntFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 10692387Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo560.235CYT0-0.200913-00NiCytoplasmic920
PPX_ECOLIP0AFL6
ppx
b2502 JW2487 cytoplasmic peripheral IM protein, Exopolyphosphatase PpxCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicIM (predicted)yesyesNo290.112CYT0-0.200913-00NiCytoplasmic1047
FADB_ECOLIP21177
fadB

oldB

oldBb3846 JW3822 cytoplasmic peripheral IM protein, Fatty acid oxidation complex subunit alpha FadBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo310.13CYT0-0.200913-00NiCytoplasmic915
FADJ_ECOLIP77399
fadJ

yfcX

yfcXb2341 JW2338 cytoplasmic peripheral IM protein, Fatty acid oxidation complex subunit alpha FadJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo700.113CYT0-0.200913-00NiCytoplasmic915
FDRA_ECOLIQ47208
fdrA

ylbD

ylbDb0518 JW0506 cytoplasmic peripheral IM protein, FdrACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 7500942Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane Integral Membrane ProteinN/AyesyesNo310.11CYT0-0.200913-00NiUnknown256
FECE_ECOLIP15031
fecE
b4287 JW4247 cytoplasmic peripheral IM protein, Fe(3+) dicitrate transport ATP-binding FecECytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo620.148CYT0-0.200913-00NiCytoplasmicMembrane104
FBPC_ECOLIP37009
fbpC

afuC yagC

afuC yagCb0262 JW0254 cytoplasmic peripheral IM protein, Fe(3+) ions import ATP-binding FbpCHomologous to MalKCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AProphage Integrase (EcoGene)yesnoNo620.109CYT0-0.200913-00NiCytoplasmicMembrane742
YDHY_ECOLIP0AAL6
ydhY
b1674 JW1664 cytoplasmic peripheral IM protein, Ferredoxin-like YdhYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo280.128CYT0-0.200913-00NiCytoplasmic931
FEPC_ECOLIP23878
fepC
b0588 JW0580 cytoplasmic peripheral IM protein, Ferric enterobactin transport ATP-binding FepC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo630.171CYT0-0.200913-00NiCytoplasmicMembrane917
FHUF_ECOLIP39405
fhuF

yjjS

yjjSb4367 JW4331 cytoplasmic peripheral IM protein, Ferric iron reductase FhuF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)It has a large transmembrane helix in the middle.PMID: 14756576Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cell membrane; Peripheral membrane proteinCytoplasmicIM (predicted)yesyesNo550.117CYT0-0.200913-01Ni91-112oCytoplasmic1029
HEMH_ECOLIP23871
hemH

popA visA

popA visAb0475 JW0464 cytoplasmic peripheral IM protein, Ferrochelatase HemHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo630.136CYT0-0.200913-00NiCytoplasmic1015
SUFB_ECOLIP77522
sufB

ynhE

ynhEb1683 JW5273 cytoplasmic peripheral IM protein, FeS cluster assembly SufB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Completely soluble no evidence for hyrophobic patches. IM binding determined by immunolocalization.PMID: 11943156Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo310.117CYT0-0.200913-00NiUnknown243
SUFD_ECOLIP77689
sufD

ynhC

ynhCb1681 JW1671 cytoplasmic peripheral IM protein, FeS cluster assembly SufD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Forms complex with SufC and SufB.PMID: 10322040Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo670.127CYT0-0.200913-00NiCytoplasmic836
FIXC_ECOLIP68644
fixC

yaaS

yaaSb0043 JW0042 cytoplasmic peripheral IM protein, FixCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The protein has hydrophobic patches all over it's sequence. Could be part of an electron transfer system required for anaerobic carnitine reduction so this is consistent with a membrane association. Strong Prediction on both Phobius and TMHMM for an aminoterminal weakly hydrophobic region. Other studies propose that this sequence may be an aminoterminal signal peptide without evidence for cleavage. The fixABCX genes in Rhodospirillum Rubrum encode a putative membrane complex participating in electron transfer to nitrogenase where FixA and B are soluble proteins.However the RrFixC expressed in E.coli was localized at the membrane fraction and was not carbonate extracted suggesting that it is an integral membrane protein. From another study in Rhizobia. Primary sequence homology, the FixC family is a highly conserved flavoprotein distantly related to the mitochondrial ETF-QO (peripheral, membrane-associated ETF : ubiquinone oxidoreductase) yet lacking both hydrophobic and amphipathic transmembrane {alpha}-helices. Thus FixC family is unlikely to be stably membrane-associated.PMID: 7473063 PMID: 15028689 PMID: 14702404Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1PeriplasmicN/AyesyesNo260.179CYT0-0.200913-00Yn8-19c25/26oCytoplasmic835
SLYD_ECOLIP0A9K9
slyD
b3349 JW3311 cytoplasmic peripheral IM protein, FKBP-type peptidyl-prolyl cis-trans isomerase SlyD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo310.112CYT0-0.200913-00NiCytoplasmic10
FLIJ_ECOLIP52613
fliJ

flaO flaS

flaO flaSb1942 JW1926 cytoplasmic peripheral IM protein, FliJ protein FliJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Part of FlgN-FlgK-FliJ-FlhA complex.PMID: 22233518Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmicN/AnoyesNo360.121CYT0-0.200913-00NiCytoplasmic1089
AZOR_ECOLIP41407
azoR

acpD

acpDb1412 JW1409 cytoplasmic peripheral IM protein, FMN-dependent NADH-azoreductase AzoRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo190.135CYT0-0.200913-00Yn4-13c18/19oCytoplasmic9108
PFLB_ECOLIP09373
pflB

pfl

pflb0903 JW0886 cytoplasmic peripheral IM protein, Formate acetyltransferase 1 PflBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo480.103CYT0-0.200913-00NiCytoplasmic1030
FDOH_ECOLIP0AAJ5
fdoH
b3893 JW3864 cytoplasmic peripheral IM protein, Formate dehydrogenase-O iron-sulfur subunit FdoHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of FDH-O isoenzyme complex whose physiological role is to ensure rapid adaptation during a shift from aerobiosis to anaerobiosis. FDH-O is a membrane-bound enzyme complex composed of three subunits, alpha (FdoG), beta (FdoH), and gamma (FdoI). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 21890895 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Single-pass membrane proteinMembrane anchoredIM (predicted)yesyesNo240.119CYT0-0.200913-00NiCytoplasmicMembrane1077
HYCE_ECOLIP16431
hycE

hevE

hevEb2721 JW2691 cytoplasmic peripheral IM protein, Formate hydrogenlyase subunit 5 HycECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Nickel-containing large subunit of Hydrogenase 3 (hycABCDEF-GHI) that forms a loose association with the molyb- doenzyme formate dehydrogenase-H on the cytoplasmic face of the cytoplasmic membrane, where together they make up the formate hydrogenlyase complex.PMID: 1625581Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo270.119CYT0-0.200913-00NiCytoplasmic97
FCTA_ECOLIP69902
frc

yfdW

yfdWb2374 JW2371 cytoplasmic peripheral IM protein, Formyl-CoA:oxalate CoA-transferase FrcCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Hypothesized to interact with the oxalate/formate antiporter and to oxalyl-CoA decarboxylase to form a large complex. 3D structure formyl-CoA transferase in Oxalobacter formigenes reveals a CoA binding site at the interface of an interlocked dimerPMID: 12839984.PMID: 12839984Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo240.119CYT0-0.200913-00NiCytoplasmic981
ALF1_ECOLIP0A991
fbaB

dhnA

dhnAb2097 JW5344 cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 1 FbaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo640.115CYT0-0.200913-00NiUnknown24
ALF_ECOLIP0AB71
fbaA

fba fda

fba fdab2925 JW2892 cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 2 FbaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 2649077PMID: 23230279 PMID: 2649077Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)N/ATFyesyesNo460.11CYT0-0.200913-00NiCytoplasmic969
FUMA_ECOLIP0AC33
fumA
b1612 JW1604 cytoplasmic peripheral IM protein, Fumarate hydratase class I, aerobic FumACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo570.116CYT0-0.200913-00NiCytoplasmic937
FUMC_ECOLIP05042
fumC
b1611 JW1603 cytoplasmic peripheral IM protein, Fumarate hydratase class II FumCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo570.12CYT0-0.200913-00NiCytoplasmic1064
FRDA_ECOLIP00363
frdA
b4154 JW4115 cytoplasmic peripheral IM protein, Fumarate reductase flavosubunit FrdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)FrdA and FrdB Fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). PMCID: PMC323229 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMCID: PMC323229 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo280.271SpI271.50227-00Yn8-18c27/28oCytoplasmicMembrane950
FRDB_ECOLIP0AC47
frdB
b4153 JW4114 cytoplasmic peripheral IM protein, Fumarate reductase iron-sulfur subunit FrdBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)FrdA and FrdB fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). PMCID: PMC323229 PMID: 8419359 PMID: 6751816 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMCID: PMC323229 PMID: 8419359 PMID: 6751816 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionnoyesNo280.105CYT0-0.200913-00NiCytoplasmicMembrane991
GATD_ECOLIP0A9S3
gatD
b2091 JW2075 cytoplasmic peripheral IM protein, Galactitol 1-phosphate 5-dehydrogenase GatDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)TFnoyesNo530.115CYT0-0.200913-00NiCytoplasmic953
MGLA_ECOLIP0AAG8
mglA
b2149 JW2136 cytoplasmic peripheral IM protein, Galactose/methyl galactoside import ATP-binding MglA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 15919657Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo290.181CYT0-0.200913-00NiCytoplasmicMembrane1014
PROA_ECOLIP07004
proA
b0243 JW0233 cytoplasmic peripheral IM protein, Gamma-glutamyl phosphate reductase ProACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo480.168CYT0-0.200913-00NiCytoplasmic10118
NAGB_ECOLIP0A759
nagB

glmD

glmDb0678 JW0664 cytoplasmic peripheral IM protein, Glucosamine-6-phosphate deaminase NagBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (219 219) PMID: 8240271PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo320.107CYT0-0.200913-00NiCytoplasmic828
DCEB_ECOLIP69910
gadB
b1493 JW1488 cytoplasmic peripheral IM protein, Glutamate decarboxylase beta GadBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls.PMID: 12912902Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo280.112CYT0-0.200913-00NiCytoplasmic1014
GLTB_ECOLIP09831
gltB

aspB

aspBb3212 JW3179 cytoplasmic peripheral IM protein, Glutamate synthase (NADPH) large chain GltBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo480.135CYT0-0.200913-00NiCytoplasmic9
GSA_ECOLIP23893
hemL

gsa popC

gsa popCb0154 JW0150 cytoplasmic peripheral IM protein, Glutamate-1-semialdehyde 2,1-aminomutase HemLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo260.222CYT0-0.200913-00NiCytoplasmic933
GLTL_ECOLIP0AAG3
gltL
b0652 JW0647 cytoplasmic peripheral IM protein, Glutamate/aspartate import ATP-binding GltLCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.108CYT0-0.200913-00NiCytoplasmicMembrane943
GLNQ_ECOLIP10346
glnQ
b0809 JW0794 cytoplasmic peripheral IM protein, Glutamine transport ATP-binding GlnQ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo420.105CYT0-0.200913-00NiCytoplasmicMembrane1097
GLMS_ECOLIP17169
glmS
b3729 JW3707 cytoplasmic peripheral IM protein, Glutamine--fructose-6-phosphate aminotransferase GlmSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicIM (predicted)TFnoyesNo590.122CYT0-0.200913-00NiCytoplasmic1091
HEM1_ECOLIP0A6X1
hemA
b1210 JW1201 cytoplasmic peripheral IM protein, Glutamyl-tRNA reductase HemACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unitPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo440.116CYT0-0.200913-00NiCytoplasmic923
GLRX3_ECOLIP0AC62
grxC

yibM

yibMb3610 JW3585 cytoplasmic peripheral IM protein, Glutaredoxin 3 GrxCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (12 15) PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo300.112CYT0-0.200913-00NiUnknown279
GLRX4_ECOLIP0AC69
grxD

ydhD

ydhDb1654 JW1646 cytoplasmic peripheral IM protein, Glutaredoxin 4 GrxDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 15833738Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo420.264CYT0-0.200913-00NiUnknown290
GSIA_ECOLIP75796
gsiA

yliA

yliAb0829 JW5897 cytoplasmic peripheral IM protein, Glutathione import ATP-binding GsiA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo620.122CYT0-0.200913-00NiCytoplasmicMembrane9
KEFF_ECOLIP0A754
kefF

yabF

yabFb0046 JW0045 cytoplasmic peripheral IM protein, Glutathione-regulated potassium-efflux system ancillary KefF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from <i>Escherichia coli</i>, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity.PMID: 17679694Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicIM (predicted)yesyesNo170.127CYT0-0.200913-00NiCytoplasmic934
KEFG_ECOLIP0A756
kefG

yheR

yheRb3351 JW3314 cytoplasmic peripheral IM protein, Glutathione-regulated potassium-efflux system ancillary KefG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from Escherichia coli, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 17679694 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicIM (predicted)yesyesNo230.13CYT0-0.200913-00NiCytoplasmic9
G3P1_ECOLIP0A9B2
gapA
b1779 JW1768 cytoplasmic peripheral IM protein, Glyceraldehyde-3-phosphate dehydrogenase A GapACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo210.121CYT0-0.200913-00NiCytoplasmic973
GLPK_ECOLIP0A6F3
glpK
b3926 JW3897 cytoplasmic peripheral IM protein, Glycerol kinase GlpKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo220.143CYT0-0.200913-00NiCytoplasmic921
PLSB_ECOLIP0A7A7
plsB
b4041 JW4001 cytoplasmic peripheral IM protein, Glycerol-3-phosphate acyltransferase PlsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid metabolism complex PlsB-PssA-YbgC-acp Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16294310 PMID: 4943977 PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo270.194CYT0-0.200913-00NiCytoplasmicMembrane10
YEHX_ECOLIP33360
yehX
b2129 JW2117 cytoplasmic peripheral IM protein, Glycine betaine uptake system ATP-binding YehX Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo420.103CYT0-0.200913-00NiCytoplasmicMembrane78
PROV_ECOLIP14175
proV
b2677 JW2652 cytoplasmic peripheral IM protein, Glycine betaine/proline betaine transport system ATP-binding ProV Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo240.108CYT0-0.200913-00NiCytoplasmicMembrane1073
GCSP_ECOLIP33195
gcvP
b2903 JW2871 cytoplasmic peripheral IM protein, Glycine dehydrogenase GcvPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo600.111CYT0-0.200913-01No751-770iCytoplasmic921
SYGA_ECOLIP00960
glyQ

glyS(A)

glyS(A)b3560 JW3531 cytoplasmic peripheral IM protein, Glycine--tRNA ligase alpha subunit GlyQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/ATFnoyesNo570.112CYT0-0.200913-00NiCytoplasmic959
SYGB_ECOLIP00961
glyS

glyS(B)

glyS(B)b3559 JW3530 cytoplasmic peripheral IM protein, Glycine--tRNA ligase beta subunit GlySCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo650.169CYT0-0.200913-00NiCytoplasmic1077
GLGA_ECOLIP0A6U8
glgA
b3429 JW3392 cytoplasmic peripheral IM protein, Glycogen synthase GlgACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo320.162CYT0-0.200913-00NiCytoplasmic910
GUAA_ECOLIP04079
guaA
b2507 JW2491 cytoplasmic peripheral IM protein, GMP synthase GuaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo450.122CYT0-0.200913-00NiCytoplasmic9
GNSA_ECOLIP0AC92
gnsA

yccL

yccLb4517 JW0976 cytoplasmic peripheral IM protein, GnsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo500.102CYT0-0.200913-00NiUnknown2
GRPE_ECOLIP09372
grpE
b2614 JW2594 cytoplasmic peripheral IM protein, GrpECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 23230279 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo350.101CYT0-0.200913-00NiCytoplasmic9
RELA_ECOLIP0AG20
relA
b2784 JW2755 cytoplasmic peripheral IM protein, GTP pyrophosphokinase RelACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo370.119CYT0-0.200913-00NiCytoplasmic875
TYPA_ECOLIP32132
typA

bipA yihK

bipA yihKb3871 JW5571 cytoplasmic peripheral IM protein, GTP-binding TypA/BipA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo370.112CYT0-0.200913-00NiCytoplasmicMembrane788
ARGK_ECOLIP27254
argK

ygfD

ygfDb2918 JW2885 cytoplasmic peripheral IM protein, GTPase ArgK ArgKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Appears to phosphorylate the periplasmic binding proteins of the lysine, arginine, ornithine transport system as well as the arginine, ornithine transport systemPMID: 2136858.PMID: 2136858Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo260.12CYT0-0.200913-00NiCytoplasmicMembrane1023
HFLX_ECOLIP25519
hflX
b4173 JW4131 cytoplasmic peripheral IM protein, GTPase HflX Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The hflALocus was found to comprise three genes, hflX, hflK, and hflC. HflK and HflC are membrane proteins and form a mem- brane-bound protease complex along with FtsH(HflB), and they are thought to act as modulators of the function of HflB. The role of the HflX GTPase/ATPase is unclear but has been shown to bind to ribosomes. When overexpressed, a portion of HflXProtein appeared in the membrane fraction, while larger amounts fractionated with the cytoplasmic pool (unpublished data cited in PMID: 19181811). In Corynebacterium glutamicum, HflX has been found to occur equally in the cytoplasm and in the membrane fractions (PMID: 15978086). Has no transmembrane region either in E. coli or in C. glutamicum. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19181811 PMID: 15978086 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo520.116CYT0-0.200913-00NiCytoplasmic852
OBG_ECOLIP42641
obgE

cgtA obg yhbZ

cgtA obg yhbZb3183 JW3150 cytoplasmic peripheral IM protein, GTPase ObgE/CgtA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associates 30S, 50S,16S, and 23S rRNA. PMID: 15292126 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 21529161 PMID: 15292126Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo250.112CYT0-0.200913-00NiCytoplasmic893
HFLD_ECOLIP25746
hflD

ycfC

ycfCb1132 JW5165 cytoplasmic peripheral IM protein, High frequency lysogenization HflDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Downregulates lambda lysogenization;Interacts with cIIProtein; first gene in purB operon Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 11278968 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo540.157CYT0-0.200913-00NiUnknown219
LIVF_ECOLIP22731
livF
b3454 JW3419 cytoplasmic peripheral IM protein, High-affinity branched-chain amino acid transport ATP-binding LivF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo420.111CYT0-0.200913-00NiCytoplasmicMembrane715
LIVG_ECOLIP0A9S7
livG
b3455 JW3420 cytoplasmic peripheral IM protein, High-affinity branched-chain amino acid transport ATP-binding LivG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo210.325CYT0-0.200913-00NiCytoplasmic911
HIS7_ECOLIP06987
hisB
b2022 JW2004 cytoplasmic peripheral IM protein, Histidine biosynthesis bifunctional HisB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo240.105CYT0-0.200913-00NiCytoplasmic1066
HISP_ECOLIP07109
hisP
b2306 JW2303 cytoplasmic peripheral IM protein, Histidine transport ATP-binding HisP Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo310.431CYT0-0.200913-00NiCytoplasmicMembrane1067
SYH_ECOLIP60906
hisS
b2514 JW2498 cytoplasmic peripheral IM protein, Histidine--tRNA ligase HisSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo200.103CYT0-0.200913-00NiCytoplasmic1059
HTRL_ECOLIP25666
htrL

yibB

yibBb3618 JW5644 cytoplasmic peripheral IM protein, HtrLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo450.131CYT0-0.200913-00NiCytoplasmic850
MBHL_ECOLIP0ACD8
hyaB
b0973 JW0955 cytoplasmic peripheral IM protein, Hydrogenase-1 large chain HyaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Peripherar Inner Membrane Cyto side (Potential) Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. PMID: 22769583 PMID: 2180913PMID: 22769583 PMID: 2180913Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinPeriplasmic (Experimental)N/AyesyesNo490.102CYT0-0.200913-00NiCytoplasmicMembrane109
HYFG_ECOLIP77329
hyfG
b2487 JW2472 cytoplasmic peripheral IM protein, Hydrogenase-4 component G HyfGPhobius predicts a slight hydrophobic area within the body proteinCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo270.133CYT0-0.200913-00NiCytoplasmic9
IMDH_ECOLIP0ADG7
guaB

guaR

guaRb2508 JW5401 cytoplasmic peripheral IM protein, Inosine-5'-monophosphate dehydrogenase GuaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Prediction of one very weak transmembrane helix.PMID: 23230279 PMID: 15919657Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo290.14CYT0-0.200913-00NiCytoplasmic949
SUHB_ECOLIP0ADG4
suhB

ssyA

ssyAb2533 JW2517 cytoplasmic peripheral IM protein, Inositol-1-monophosphatase SuhBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SuhB forms a tight complex with RNA polymerase (RNA pol) that inhibits the IMPase catalytic activity of SuhBPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo210.179CYT0-0.200913-00NiCytoplasmic986
MLAF_ECOLIP63386
mlaF

yrbF

yrbFb3195 JW3162 cytoplasmic peripheral IM protein, Interphospholipid transport system ATP-binding MlaF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo640.109CYT0-0.200913-00NiCytoplasmicMembrane1023
MLAB_ECOLIP64602
mlaB

yrbB

yrbBb3191 JW5535 cytoplasmic peripheral IM protein, Interphospholipid transport system binding MlaB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Probable phospholipid ABC transporter-binding protein mlaB Probable phospholipid ABC transporter-binding protein mlaB Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo660.108CYT0-0.200913-00NiUnknown2
RSXB_ECOLIP77223
rsxB

rnfB ydgM

rnfB ydgMb1628 JW1620 cytoplasmic peripheral IM protein, Ion-translocating oxidoreductase complex subunit B RsxBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Electron transport. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane PeriplasmicN/AyesyesNo310.278SpI304.42273-10Yn3-14c19/20oCytoplasmicMembrane1022
RSXC_ECOLIP77611
rsxC

rnfC ydgN

rnfC ydgNb1629 JW1621 cytoplasmic peripheral IM protein, Ion-translocating oxidoreductase complex subunit C RsxCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo540.101CYT0-0.200913-00NiCytoplasmic8
FHUC_ECOLIP07821
fhuC
b0151 JW0147 cytoplasmic peripheral IM protein, Iron(3+)-hydroxamate import ATP-binding FhuCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1551849 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo390.111CYT0-0.200913-00NiCytoplasmicMembrane1045
ISCU_ECOLIP0ACD4
iscU

nifU yfhN

nifU yfhNb2529 JW2513 cytoplasmic peripheral IM protein, Iron-sulfur cluster assembly scaffold IscU Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo200.105CYT0-0.200913-00NiCytoplasmic997
ERPA_ECOLIP0ACC3
erpA

yadR

yadRb0156 JW0152 cytoplasmic peripheral IM protein, Iron-sulfur cluster insertion ErpA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo490.128CYT0-0.200913-00NiCytoplasmic984
YTFE_ECOLIP69506
ytfE
b4209 JW4167 cytoplasmic peripheral IM protein, Iron-sulfur cluster repair YtfE Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo210.13CYT0-0.200913-00NiCytoplasmic10103
IDH_ECOLIP08200
icd

icdA icdE

icdA icdEb1136 JW1122 cytoplasmic peripheral IM protein, Isocitrate dehydrogenase (NADP) IcdCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)TFyesyesNo470.119CYT0-0.200913-00NiCytoplasmic996
ACEA_ECOLIP0A9G6
aceA

icl

iclb4015 JW3975 cytoplasmic peripheral IM protein, Isocitrate lyase AceACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo300.126CYT0-0.200913-00NiCytoplasmic1042
ILVC_ECOLIP05793
ilvC
b3774 JW3747 cytoplasmic peripheral IM protein, Ketol-acid reductoisomerase IlvCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo290.107CYT0-0.200913-00NiCytoplasmic1097
ALKH_ECOLIP0A955
eda

hga kdgA

hga kdgAb1850 JW1839 cytoplasmic peripheral IM protein, KHG/KDPG aldolase EdaCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo300.126CYT0-0.200913-00NiCytoplasmic1077
LHGO_ECOLIP37339
lhgO

ygaF

ygaFb2660 JW2635 cytoplasmic peripheral IM protein, L-2-hydroxyglutarate oxidase LhgO Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted), Soluble IM FractionyesyesNo210.14CYT0-0.200913-00NiCytoplasmic915
YECC_ECOLIP37774
yecC
b1917 JW1902 cytoplasmic peripheral IM protein, L-cystine transport system ATP-binding YecC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo340.123CYT0-0.200913-00NiCytoplasmicMembrane737
LLDD_ECOLIP33232
lldD

lctD

lctDb3605 JW3580 cytoplasmic peripheral IM protein, L-lactate dehydrogenase LldDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)LldD is a cytochrome. There is a strong experimental indication that it is a membrane bound protein although no transmembrane helix is predicted PMID: 18473. PMID: 8407843 PMID: 6997721 PMID: 18473 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18473 PMID: 15919657 PMID: 8407843 PMID: 6997721 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Membrane associated (Experimental)IM (predicted-detected)yesyesNo320.129CYT0-0.200913-00NiCytoplasmic94
SDHL_ECOLIP16095
sdaA
b1814 JW1803 cytoplasmic peripheral IM protein, L-serine dehydratase 1 SdaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo200.136CYT0-0.200913-00NiCytoplasmic971
TDCB_ECOLIP0AGF6
tdcB
b3117 JW3088 cytoplasmic peripheral IM protein, L-threonine dehydratase catabolic TdcB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 PMID: 25403562Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)YfgMyesyesNo230.152CYT0-0.200913-00NiCytoplasmic972
LACI_ECOLIP03023
lacI
b0345 JW0336 cytoplasmic peripheral IM protein, Lactose operon repressor LacICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Regulates lac operon transcription when in homotetrameric form.PMID: 21936772 PMID: 15863616 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo330.136CYT0-0.200913-00NiCytoplasmic1032
LPXB_ECOLIP10441
lpxB

pgsB

pgsBb0182 JW0177 cytoplasmic peripheral IM protein, Lipid-A-disaccharide synthase LpxBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomerPMID: 19883124. It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer. PMID: 19883124 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19883124 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo230.146CYT0-0.200913-00NiCytoplasmic1047
LCFA_ECOLIP69451
fadD

oldD

oldDb1805 JW1794 cytoplasmic peripheral IM protein, Long-chain-fatty-acid--CoA ligase FadDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Acyl-coenzyme A (CoA) synthetase that may interact with the inner membrane. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicN/AyesyesNo630.12CYT0-0.200913-00NiCytoplasmic936
PLDB_ECOLIP07000
pldB
b3825 JW5584 cytoplasmic peripheral IM protein, Lysophospholipase L2 PldBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Gene and characteristics of deduced amino acid sequence of lysophospholipase L2. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo340.104CYT0-0.200913-00NiCytoplasmicMembrane736
MDH_ECOLIP61889
mdh
b3236 JW3205 cytoplasmic peripheral IM protein, Malate dehydrogenase MdhCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 7028159 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15976458 PMID: 7028159 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)N/ATFyesyesNo180.251SpI170.386899-00Yn3-12c17/18oUnknown6
MQO_ECOLIP33940
mqo

yojH

yojHb2210 JW2198 cytoplasmic peripheral IM protein, Malate:quinone oxidoreductase MqoCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)High signal PProbability and lipoP SPIPrediction; weak TmHMMPrediction definite membrane association according to literature. MQOIs a flavin adenine dinucleotide (FAD)- and lipid-dependent peripheral membrane protein catalyzing the oxidation ofL-malate to oxaloacetate. PMID: 11092847 PMID: 368072 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 11092847 PMID: 368072 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Membrane associated (Experimental)IM (predicted)yesyesYes270.278SpI218.41159-00Yn4-15c21/22oUnknown617
MALK_ECOLIP68187
malK
b4035 JW3995 cytoplasmic peripheral IM protein, Maltose/maltodextrin import ATP-binding MalK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)ATPase of maltose uptake system. MalF functions in maltose transport as a 1:1:2 complex with the integral inner membrane protein MalG and the peripheral inner membrane protein MalK Crystal structure of a catalytic intermediate of the maltose transporter. PMID: 18033289PMID: 16079137 PMID: 18456666Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo150.106CYT0-0.200913-00NiCytoplasmicMembrane1019
PTMA_ECOLIP69824
cmtB
b2934 JW2901 cytoplasmic peripheral IM protein, Mannitol-specific cryptic phosphotransferase enzyme IIA component CmtBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)PMID: 9525892Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo190.136CYT0-0.200913-00NiUnknown296
FADK_ECOLIP38135
fadK

ydiD

ydiDb1701 JW5910 cytoplasmic peripheral IM protein, Medium-chain fatty-acid--CoA ligase FadKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homologous to FadD13 protein of Mycobacterium that is peripheralPMID: 22560731Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane protein CytoplasmicN/AnoyesNo370.131CYT0-0.200913-00NiCytoplasmic9
MAP1_ECOLIP0AE18
map
b0168 JW0163 cytoplasmic peripheral IM protein, Methionine aminopeptidase MapCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300PMID: 23230279 PMID: 17272300Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo220.108CYT0-0.200913-00NiCytoplasmic963
METN_ECOLIP30750
metN

abc

abcb0199 JW0195 cytoplasmic peripheral IM protein, Methionine import ATP-binding MetN Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo420.139CYT0-0.200913-00NiCytoplasmicMembrane923
METH_ECOLIP13009
metH
b4019 JW3979 cytoplasmic peripheral IM protein, Methionine synthase MetHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo350.124CYT0-0.200913-00NiCytoplasmic9
SYM_ECOLIP00959
metG
b2114 JW2101 cytoplasmic peripheral IM protein, Methionine--tRNA ligase MetGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/ATFyesyesNo200.115CYT0-0.200913-00NiCytoplasmic1049
MOAB_ECOLIP0AEZ9
moaB

chlA2

chlA2b0782 JW0765 cytoplasmic peripheral IM protein, Molybdenum cofactor biosynthesis B MoaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo240.114CYT0-0.200913-00NiCytoplasmic9105
MODC_ECOLIP09833
modC

chlD narD

chlD narDb0765 JW0748 cytoplasmic peripheral IM protein, Molybdenum import ATP-binding ModC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo350.128CYT0-0.200913-00NiCytoplasmicMembrane10
MTFA_ECOLIP76346
mtfA

yeeI

yeeIb1976 JW1958 cytoplasmic peripheral IM protein, MtfACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Cytoplasmic transciption factor that shuttles from chromosome to the membranes as a peripheral protein on the PtsG transporter. Specifically it binds to the unphosphorylated, trnasport-mediating form of the EIIBCGlc subunit. PMID: 16855233PMID: 15063852 PMID: 16855233Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo360.109CYT0-0.200913-00NiCytoplasmic887
PTFX1_ECOLIP77439
fryA

ypdD

ypdDb2383 JW2380 cytoplasmic peripheral IM protein, Multiphosphoryl transfer 1 FryACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Multiphosphoryl transfer protein 1 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo170.156CYT0-0.200913-00Yn2-10c16/17oCytoplasmic938
PTFX2_ECOLIP32670
ptsA

frwA yijH

frwA yijHb3947 JW5555 cytoplasmic peripheral IM protein, Multiphosphoryl transfer 2 PtsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Multiphosphoryl transfer protein 2 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo180.56CYT0-0.200913-00NiCytoplasmic968
PTFAH_ECOLIP69811
fruB

fpr fruF

fpr fruFb2169 JW2156 cytoplasmic peripheral IM protein, Multiphosphoryl transfer FruBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8013873 PMID: 2841594PMID: 15911532 PMID: 8013873 PMID: 2841594Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionnoyesNo390.108CYT0-0.200913-00NiCytoplasmic1091
MTOX_ECOLIP40874
solA
b1059 JW1046 cytoplasmic peripheral IM protein, N-methyl-L-tryptophan oxidase SolACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo160.149CYT0-0.200913-00NiCytoplasmic973
NADK_ECOLIP0A7B3
nadK

yfjB yfjE

yfjB yfjEb2615 JW2596 cytoplasmic peripheral IM protein, NAD kinase NadKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Fluorescent fusions of ppnK have been monitored via fluorescence microscopy. ppnK distributed irregularly throughout the cytoplasm, with some clusters formed.PMID: 17272300Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo200.111CYT0-0.200913-00NiCytoplasmic932
DHNA_ECOLIP00393
ndh
b1109 JW1095 cytoplasmic peripheral IM protein, NADH dehydrogenase NdhCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains amphipathic C-terminal anchor (390-406). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 20933494 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membraneCytoplasmicIM (predicted)yesyesNo220.114CYT0-0.200913-00NiCytoplasmicMembrane1035
NUOB_ECOLIP0AFC7
nuoB
b2287 JW5875 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit B NuoBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Crystal structure of the entire respiratory complex I PMID: 23417064 Crystal structure of the entire respiratory complex I PMID: 21822288 PMID: 7607227 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19189973 PMID: 15037611 PMID: 23417064 PMID: 21822288 PMID: 7607227 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmic (Experimental)IM (predicted)yesyesNo620.1CYT0-0.200913-00NiCytoplasmicMembrane978
NUOCD_ECOLIP33599
nuoC

nuoCD nuoD

nuoCD nuoDb2286 JW5375 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit C/D NuoCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 7607227 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 7607227 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)IM (predicted)TFyesyesNo390.108CYT0-0.200913-00NiCytoplasmic917
NUOE_ECOLIP0AFD1
nuoE
b2285 JW2280 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit E NuoECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complexPMID: 19737520. Thermus homologue: Q56221 (NQO2_THET8). Copurifies with trigger factor in pull down assaysPMID: 19737520. PMID: 11416161 PMID: 7607227 PMID: 7690854 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23543743 PMID: 11416161 PMID: 7607227 PMID: 7690854 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo670.103CYT0-0.200913-00NiCytoplasmic931
NUOF_ECOLIP31979
nuoF
b2284 JW2279 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit F NuoFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.cThermus homologue: Q56222 (NQO1_THET8) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo570.101CYT0-0.200913-00NiCytoplasmic911
NUOG_ECOLIP33602
nuoG
b2283 JW2278 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit G NuoGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. PMID: 11416161 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 11416161 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo570.143CYT0-0.200913-00NiCytoplasmicMembrane1017
NUOI_ECOLIP0AFD6
nuoI
b2281 JW2276 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit I NuoICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) PMID: 21822288 PMID: 23417064 PMID: 15037611 PMID: 19189973 PMID: 11416161 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo270.17CYT0-0.200913-01No6-26iCytoplasmic972
MAO2_ECOLIP76558
maeB

ypfF

ypfFb2463 JW2447 cytoplasmic peripheral IM protein, NADP-dependent malic enzyme MaeBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo450.165CYT0-0.200913-00NiCytoplasmic981
SEQA_ECOLIP0AFY8
seqA
b0687 JW0674 cytoplasmic peripheral IM protein, Negative modulator of initiation of replication SeqACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Membrane-associated oriC-binding machinery of E. coli is a multiprotein complex that includes the SeqA and SeqB proteins. PMID: 9660922 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 9736699 PMID: 9660922 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo430.12CYT0-0.200913-00NiCytoplasmic813
NADE_ECOLIP18843
nadE

efg ntrL

efg ntrLb1740 JW1729 cytoplasmic peripheral IM protein, NH(3)-dependent NAD(+) synthetase NadECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo200.146CYT0-0.200913-00NiCytoplasmic9
NIKD_ECOLIP33593
nikD
b3479 JW3444 cytoplasmic peripheral IM protein, Nickel import ATP-binding NikD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo480.168CYT0-0.200913-00NiCytoplasmicMembrane745
NIKE_ECOLIP33594
nikE
b3480 JW3445 cytoplasmic peripheral IM protein, Nickel import ATP-binding NikE Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo680.149CYT0-0.200913-00NiCytoplasmicMembrane941
GLNK_ECOLIP0AC55
glnK

ybaI

ybaIb0450 JW0440 cytoplasmic peripheral IM protein, Nitrogen regulatory P-II 2 GlnKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Encodes a member of the PII signal transduction protein family, proteins that regulate enzyme activity and gene expression in response to the intracellular nitrogen status; cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the the ammonium transporter AmtB.PMID: 11847102 PMID: 9720863 PMID: 10231487 PMID: 14668330Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo670.123CYT0-0.200913-00NiCytoplasmicMembrane741
PTSN_ECOLIP69829
ptsN

rpoP yhbI

rpoP yhbIb3204 JW3171 cytoplasmic peripheral IM protein, Nitrogen regulatory PtsNCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)enzyme IIA(Ntr) of the nitrogen PTS regulates the intracellular concentration of K(+) by direct interaction with TrkA and KdpD. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 17289841 PMID: 21605551 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo260.129CYT0-0.200913-00NiCytoplasmic1082
NDK_ECOLIP0A763
ndk
b2518 JW2502 cytoplasmic peripheral IM protein, Nucleoside diphosphate kinase NdkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoside diphosphate kinase (EC 2.7.4.6) (Ndk) is a ubiquitous enzyme functioning in the intracellular distribution of terminal phosphate bond energy among the various nucleotides used in synthetic and regulatory functions in cellsPMID: 8606147. Based on studies in Pseudomonas and many other systems including mitochondria, the uracil-processing DNA repair nuclease ndk is known to interact to plasma membranes and mitochondrial inner membranePMID: 15096615. Binding is regulated by nucleotidesPMID: 19146889. In Pseudomonas, an intracellular protease cleaves the 16-kDa form of Ndk to a 12-kDa form that undergoes autophosphorylation with an efficiency almost identical to that of the 16-kDa form. The 12-kDa form is predominantly associated with the cell membrane fraction, whereas the 16-kDa form is predominantly cytoplasmic. In the membrane-associated state, the 12-kDa form of Ndk was found to synthesize GTP in preference to other nucleoside triphosphates. The formation of the 12-kDa form of Ndk and its association with the cell membrane were found to be related to the growth stage of <i>P.aeruginosa</i>, with less than 1% of the 12-kDa Ndk detectable in the membrane fraction at early log phase in comparison with the levels present at late stationary phasePMID: 8606147. Cytosolic Ndks bind directly to membrane lipids in a dynamic process and can be fully reconstituted with phospholipids and recombinant Ndk PMID: 19146889. Ndks may act as a reservoir of high energy phosphate, supporting the operation of membrane-based processes that utilize nucleotides other than ATP, such as intracellular traffic and phospholipid biosynthesis. Trigger factor-sensitive aggregationPMID: 19737520. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19146889 PMID: 8606147 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFnoyesNo170.158CYT0-0.200913-00NiCytoplasmic1064
OPDA_ECOLIP27298
prlC

opdA

opdAb3498 JW3465 cytoplasmic peripheral IM protein, Oligopeptidase A PrlCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo260.115CYT0-0.200913-00NiCytoplasmic1094
OPPD_ECOLIP76027
oppD
b1246 JW1238 cytoplasmic peripheral IM protein, Oligopeptide transport ATP-binding OppD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Oligopeptide permease (Opp) transporters are located in the plasma membrane and have functions generally related to the uptake of peptides from the extracellular environment. OppD is the ATP-binding protein. PMID: 24793159 Based on sequence similarity, OppB and OppC are the membrane components of the ABC transporter, and OppD and OppF are the ATP-binding components of the ABC transporter PMID: 1738314 Opp-LacZ hybrid proteins were used to show that OppB, OppC and OppD are membrane-associated proteins. PMID: 2821267 PMID: 16045610 [22087229 ] PMID: 3926486 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 24793159 PMID: 1738314 PMID: 2821267 PMID: 16045610 PMID: 22087229 PMID: 3926486 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo690.132CYT0-0.200913-00NiCytoplasmicMembrane1018
OPPF_ECOLIP77737
oppF
b1247 JW1239 cytoplasmic peripheral IM protein, Oligopeptide transport ATP-binding OppF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo700.124CYT0-0.200913-00NiCytoplasmicMembrane1017
ORN_ECOLIP0A784
orn

o204a yjeR

o204a yjeRb4162 JW5740 cytoplasmic peripheral IM protein, Oligoribonuclease OrnCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 24438330Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo480.121CYT0-0.200913-00NiCytoplasmic9103
BETA_ECOLIP17444
betA
b0311 JW0303 cytoplasmic peripheral IM protein, Oxygen-dependent choline dehydrogenase BetACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 3512525 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicN/AnoyesNo190.163CYT0-0.200913-00NiCytoplasmicMembrane108
NFSB_ECOLIP38489
nfsB

dprA nfnB nfsI ntr

dprA nfnB nfsI ntrb0578 JW0567 cytoplasmic peripheral IM protein, Oxygen-insensitive NAD(P)H nitroreductase NfsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 12788749Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)IM (predicted)yesyesNo180.166CYT0-0.200913-00NiUnknown286
PCNB_ECOLIP0ABF1
pcnB
b0143 JW5808 cytoplasmic peripheral IM protein, PcnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)RNA degradosome component/adds polyA tails to mRNAs targeted for degradation. The pcnB gene product, is the main enzyme responsible for RNA polyadenylation in E. coli. Polyadenylated RNA molecules are rapidly degraded by a multiprotein complex called RNA degradosome. PAP I localizes in the cytosol but also, like the RNA degradosome, in cellular membranes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15737627 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicIM (predicted)yesyesNo260.111CYT0-0.200913-00NiCytoplasmic923
DHAM_ECOLIP37349
dhaM

ycgC

ycgCb1198 JW5185 cytoplasmic peripheral IM protein, PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo360.134CYT0-0.200913-00NiCytoplasmic974
DEF_ECOLIP0A6K3
def

fms

fmsb3287 JW3248 cytoplasmic peripheral IM protein, Peptide deformylase DefCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo420.113CYT0-0.200913-00NiCytoplasmic966
PPIB_ECOLIP23869
ppiB
b0525 JW0514 cytoplasmic peripheral IM protein, Peptidyl-prolyl cis-trans isomerase B PpiBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 9268657Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)N/AyesyesNo100.101CYT0-0.200913-00NiCytoplasmic1064
PSPA_ECOLIP0AFM6
pspA
b1304 JW1297 cytoplasmic peripheral IM protein, Phage shock A PspACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Forms large oligomers and binds directly to phospholipids (preferentially phosphatidylserine and phosphatidylglycerol). PMC3709507 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19555453 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AProphage TransposaseyesnoNo230.112CYT0-0.200913-00NiCytoplasmicMembrane10113
PSPD_ECOLIP0AFV8
pspD
b1307 JW1300 cytoplasmic peripheral IM protein, Phage shock D PspDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 12562786 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AProphage TransposasenonoNo640.182CYT0-0.200913-00NiUnknown234
SYFA_ECOLIP08312
pheS
b1714 JW5277 cytoplasmic peripheral IM protein, Phenylalanine--tRNA ligase alpha subunit PheSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo190.166CYT0-0.200913-00NiCytoplasmic1012
SYFB_ECOLIP07395
pheT
b1713 JW1703 cytoplasmic peripheral IM protein, Phenylalanine--tRNA ligase beta subunit PheTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo290.131CYT0-0.200913-00NiCytoplasmic979
PHOL_ECOLIP0A9K3
ybeZ
b0660 JW0657 cytoplasmic peripheral IM protein, PhoH-like protein YbeZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo680.101CYT0-0.200913-00NiCytoplasmic961
CHEZ_ECOLIP0A9H9
cheZ
b1881 JW1870 cytoplasmic peripheral IM protein, phosphatase CheZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 12644507PMID: 19766000 PMID: 12644507Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo660.129CYT0-0.200913-00NiCytoplasmic10107
PLSX_ECOLIP27247
plsX
b1090 JW5156 cytoplasmic peripheral IM protein, Phosphate acyltransferase PlsXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo240.213CYT0-0.200913-00NiCytoplasmic915
PSTB_ECOLIP0AAH0
pstB

phoT

phoTb3725 JW3703 cytoplasmic peripheral IM protein, Phosphate import ATP-binding PstB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 3881386 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo410.133CYT0-0.200913-00NiCytoplasmicMembrane1023
PHOU_ECOLIP0A9K7
phoU

nmpA

nmpAb3724 JW3702 cytoplasmic peripheral IM protein, Phosphate-specific transport system accessory PhoU Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 3536855 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo170.113CYT0-0.200913-00NiCytoplasmic1094
PSD_ECOLIP0A8K1
psd
b4160 JW4121 cytoplasmic peripheral IM protein, Phosphatidylserine decarboxylase proenzyme PsdCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Phosphatidylserine decarboxylase is composed of two nonidentical subunits, alpha and beta, derived from a single proenzyme. The proenzyme is processed in a post-translational event into the heterodimer. This enzyme is unique among the known pyruvoyl-dependent decarboxylases because it is membrane-associated. PMID: 3042771 The phosphatidylserine decarboxylase (EC 4.1.1.65, phosphatidyl-L-serine carboxy-lyase) of Escherichia coli is associated with the cytoplasmic membrane and requires nonionic detergent for solubilization and detection of enzymatic activity PMID: 1495415 A strain of Escherichia coli bearing a hybrid plasmid containing the psd gene, starved for isoleucine by the addition of valine, produces amounts of phosphatidyl-serine decarboxylase, a membrane-bound enzyme, about 40-fold higher than wild type. PMID: 368058 The first phospholipid biosynthetic enzyme to be purified to homogeneity was Psd of E. coli. Beginning in the late 1960s purification of membrane proteins after solubilization with detergents was an increasingly popular endeavor. PMID: 22925633 PMID: 3042771 PMID: 5543671 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 3042771 PMID: 1495415 PMID: 368058 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionnoyesNo230.185CYT0-0.200913-00NiCytoplasmicMembrane1032
CYSH_ECOLIP17854
cysH
b2762 JW2732 cytoplasmic peripheral IM protein, Phosphoadenosine phosphosulfate reductase CysHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo590.168CYT0-0.200913-00NiCytoplasmic1011
PTHP_ECOLIP0AA04
ptsH

hpr

hprb2415 JW2408 cytoplasmic peripheral IM protein, Phosphocarrier HPr PtsHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AnoyesNo220.11CYT0-0.200913-00NiCytoplasmic1088
CAPP_ECOLIP00864
ppc

glu

glub3956 JW3928 cytoplasmic peripheral IM protein, Phosphoenolpyruvate carboxylase PpcCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo480.115CYT0-0.200913-00NiCytoplasmic8
PPSA_ECOLIP23538
ppsA

pps

ppsb1702 JW1692 cytoplasmic peripheral IM protein, Phosphoenolpyruvate synthase PpsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)One very weak transmembrane region predicted. Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo560.169CYT0-0.200913-00NiCytoplasmic948
PT1P_ECOLIP37177
ptsP

ygdF ygdO

ygdF ygdOb2829 JW2797 cytoplasmic peripheral IM protein, Phosphoenolpyruvate-dependent phosphotransferase system PtsPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phosphoenolpyruvate-protein phosphotransferase ptsP Localization to the inner surface of the cytoplasmic membrane by immunoelectron microscopy of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli. PMID: 2644649PMID: 2644649Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo330.185CYT0-0.200913-00NiCytoplasmic99
PT1_ECOLIP08839
ptsI
b2416 JW2409 cytoplasmic peripheral IM protein, Phosphoenolpyruvate-phosphotransferase PtsICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 2644649 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo150.115CYT0-0.200913-00NiCytoplasmic1090
PGK_ECOLIP0A799
pgk
b2926 JW2893 cytoplasmic peripheral IM protein, Phosphoglycerate kinase PgkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo210.107CYT0-0.200913-00NiCytoplasmic10100
SIXA_ECOLIP76502
sixA

yfcW

yfcWb2340 JW2337 cytoplasmic peripheral IM protein, Phosphohistidine phosphatase SixA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Exhibits phosphohistidine phosphatase activity towards the HPt domain of the ArcB sensor involved in the multistep His-Asp phosphorelayPMID: 9489669. The structure of SixA reveals it forms a relatively shallow groove important for accommodating the kidney-shaped four-helix bundle of the HPt domain of AcrBPMID: 17628145.PMID: 9489669 PMID: 17628145 PMID: 10734219Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo570.122CYT0-0.200913-00NiUnknown279
PHNC_ECOLIP16677
phnC
b4106 JW4067 cytoplasmic peripheral IM protein, Phosphonates import ATP-binding PhnCCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo230.137CYT0-0.200913-00NiCytoplasmicMembrane1010
DEOB_ECOLIP0A6K6
deoB

drm thyR

drm thyRb4383 JW4346 cytoplasmic peripheral IM protein, Phosphopentomutase DeoBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo400.147CYT0-0.200913-00NiCytoplasmic10101
PHNP_ECOLIP16692
phnP
b4092 JW4053 cytoplasmic peripheral IM protein, Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase PhnPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 8335257Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Periplasmic (Experimental)N/AyesyesNo210.153CYT0-0.200913-00Yn3-10c15/16oCytoplasmic9
PUR7_ECOLIP0A7D7
purC
b2476 JW2461 cytoplasmic peripheral IM protein, Phosphoribosylaminoimidazole-succinocarboxamide synthase PurCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo650.127CYT0-0.200913-00NiCytoplasmic950
PPK1_ECOLIP0A7B1
ppk
b2501 JW2486 cytoplasmic peripheral IM protein, Polyphosphate kinase PpkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1331061 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane CytoplasmicN/AyesyesNo260.112CYT0-0.200913-00NiCytoplasmicMembrane107
PNP_ECOLIP05055
pnp
b3164 JW5851 cytoplasmic peripheral IM protein, Polyribonucleotide nucleotidyltransferase PnpCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Polynucleotide phosphorylase (PNPase) or Polyribonucleotide nucleotidyltransferase Is a cytoplasmic enzyme that has also been isolated in association with the inner membrane (Stenberg et al., 2005; Maddalo et al., 2011). It is not known if association is direct. The protein has weak hydrophobic patches and three regions with weak predictions from Phobius for membrane interaction. Membrane association may be via binding to RNAaseE with which PNPase interacts as part of the multienzyme complex known as the RNA degradosome. RNAseE has been shown to bind to membranes (Khemici et al., 2008). The interaction of RNase E with PNPase , brings together two enzymes important in the degradation of mRNA. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 16079137 PMID: 23230279 PMID: 24438330Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionTFyesyesNo300.323CYT0-0.200913-00NiCytoplasmic1074
WAAU_ECOLIP27242
waaU

rfaK waaK

rfaK waaKb3623 JW3598 cytoplasmic peripheral IM protein, polysaccharide 1,2-N-acetylglucosaminetransferase WaaUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 1657881Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Membrane; Peripheral membrane proteinCytoplasmicN/AnoyesNo330.113CYT0-0.200913-00NiCytoplasmic8
RFAG_ECOLIP25740
rfaG

pcsA waaG

pcsA waaGb3631 JW3606 cytoplasmic peripheral IM protein, polysaccharide core biosynthesis RfaGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo250.118CYT0-0.200913-00NiCytoplasmic930
RFAP_ECOLIP25741
rfaP

waaP

waaPb3630 JW3605 cytoplasmic peripheral IM protein, polysaccharide core heptose RfaPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo200.155CYT0-0.200913-00NiCytoplasmic933
RFAQ_ECOLIP25742
rfaQ

waaQ

waaQb3632 JW3607 cytoplasmic peripheral IM protein, polysaccharide core heptosyltransferase RfaQ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo250.11CYT0-0.200913-00NiCytoplasmic833
LPTB_ECOLIP0A9V1
lptB

yhbG

yhbGb3201 JW3168 cytoplasmic peripheral IM protein, polysaccharide export system ATP-binding LptB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 17056748 PMID: 16079137 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic(experimental)Soluble protein detected in Inner Membrane FractionyesyesNo430.117CYT0-0.200913-00NiCytoplasmicMembrane1019
RFAC_ECOLIP24173
rfaC

rfa-2 waaC yibC

rfa-2 waaC yibCb3621 JW3596 cytoplasmic peripheral IM protein, polysaccharide heptosyltransferase 1 RfaCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo500.195CYT0-0.200913-00NiCytoplasmic951
YGAU_ECOLIP0ADE6
ygaU

yzzM

yzzMb2665 JW2640 cytoplasmic peripheral IM protein, Potassium binding Kbp YgaUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane PMID: 12175925. Homologous to OsmY.PMID: 21541338 PMID: 23230279 PMID: 12878000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo100.101CYT0-0.200913-00NiUnknown289
KDPF_ECOLIP36937
kdpF
b4513 JW0687 cytoplasmic peripheral IM protein, Potassium-transporting ATPase KdpF subunit KdpFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)KdpF, could be identified on high resolution SDS-polyacrylamide gels in addition to the subunits KdpA, KdpB, and KdpC. Furthermore, it could be demonstrated that KdpF remains associated with the purified complex. As determined by mass spectrometry, this pe PMID: 9789555PMID: 21890895 PMID: 9789555Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Membrane Anchored (Experimental)IM (predicted)noyesNo230.109CYT0-0.200913-10Yn5-16c22/23oCytoplasmicMembrane1029
ARND_ECOLIP76472
arnD

pmrJ yfbH

pmrJ yfbHb2256 JW2250 cytoplasmic peripheral IM protein, Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo670.132CYT0-0.200913-00NiCytoplasmic910
SUFC_ECOLIP77499
sufC

ynhD

ynhDb1682 JW1672 cytoplasmic peripheral IM protein, Probable ATP-dependent transporter SufC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction. Homolog to import ATP-binding protein MalK. PMID: 12554644PMID: 12941942 PMID: 12554644Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo320.213CYT0-0.200913-00NiCytoplasmic1069
DDPD_ECOLIP77268
ddpD

yddP

yddPb1484 JW1479 cytoplasmic peripheral IM protein, Probable D,D-dipeptide transport ATP-binding DdpD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo250.176CYT0-0.200913-00NiCytoplasmicMembrane723
DDPF_ECOLIP77622
ddpF

yddO

yddOb1483 JW1478 cytoplasmic peripheral IM protein, Probable D,D-dipeptide transport ATP-binding DdpF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo320.115CYT0-0.200913-00NiCytoplasmicMembrane918
FETP_ECOLIP0A8P3
yggX
b2962 JW2929 cytoplasmic peripheral IM protein, Probable Fe(2+)-trafficking protein YggXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally verified gene-product. Methionine start codon is cleaved. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo410.14CYT0-0.200913-00Yn4-11c16/17oUnknown2107
ENGB_ECOLIP0A6P7
engB

yihA

yihAb3865 JW5930 cytoplasmic peripheral IM protein, Probable GTP-binding EngB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of YqiK have been monitored via fluorescence microscopy. YqiK was found to be distributed diffusely throughout cytoplasm.PMID: 17272300 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo430.126CYT0-0.200913-00NiUnknown2
FETA_ECOLIP77279
fetA

ybbL

ybbLb0490 JW0479 cytoplasmic peripheral IM protein, Probable iron export ATP-binding FetA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo330.126CYT0-0.200913-00NiCytoplasmicMembrane942
YCJR_ECOLIP76044
ycjR
b1314 JW5202 cytoplasmic peripheral IM protein, Probable ketose 3-epimerase YcjRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo470.118CYT0-0.200913-00NiCytoplasmic844
YBHF_ECOLIP0A9U1
ybhF
b0794 JW5104 cytoplasmic peripheral IM protein, Probable multidrug ABC transporter ATP-binding YbhF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo380.115CYT0-0.200913-00NiCytoplasmicMembrane742
SYP_ECOLIP16659
proS

drpA

drpAb0194 JW0190 cytoplasmic peripheral IM protein, Proline--tRNA ligase ProSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/ATFyesyesNo190.254CYT0-0.200913-00NiCytoplasmic1016
PRPE_ECOLIP77495
prpE

yahU

yahUb0335 JW0326 cytoplasmic peripheral IM protein, Propionate--CoA ligase PrpECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to FadD13 protein of Mycobacterium that is peripheralPMID: 22560731.PMID: 22560731Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo440.132CYT0-0.200913-00NiCytoplasmic913
SECB_ECOLIP0AG86
secB
b3609 JW3584 cytoplasmic peripheral IM protein, Protein-export SecBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Cytoplasmic protein that forms transient interactions with the membrane; binds to the C-terminus of SecA. Protein that acts as its membrane receptor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 9767586 PMID: 2170023 PMCID: PMC1326294 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo530.133CYT0-0.200913-00NiCytoplasmic1088
HEMG_ECOLIP0ACB4
hemG

yihB

yihBb3850 JW3827 cytoplasmic peripheral IM protein, Protoporphyrinogen IX dehydrogenase HemGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo330.11CYT0-0.200913-00NiUnknown275
PTFLA_ECOLIP32155
frvA

yiiK

yiiKb3900 JW3871 cytoplasmic peripheral IM protein, PTS system fructose-like EIIA component FrvACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Fructose-like phosphotransferase enzyme IIA component Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo190.135CYT0-0.200913-00NiCytoplasmic968
PTFB2_ECOLIP69816
frwB

yijK

yijKb3950 JW3922 cytoplasmic peripheral IM protein, PTS system fructose-like EIIB component 2 FrwBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively. SgaT represents a novel prototypical enzyme IIC that functions with SgaA and SgaB to allow phosphoryl transfer from HPr(his-P) to l-ascorbate via the phosphoryl transfer pathwayCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo210.363SpI204.62655-00NiCytoplasmic1087
PTFB3_ECOLIP32676
frwD

yijN

yijNb3953 JW3925 cytoplasmic peripheral IM protein, PTS system fructose-like EIIB component 3 FrwDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Sequence analysis indicates that an frwD encoded protein has similarity to the IIB domains of the PTS Enzymes II specific for fructose. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo210.559CYT0-0.200913-00Yn4-12c17/18oUnknown643
PTKA_ECOLIP69828
gatA
b2094 JW2078/JW2081 cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIA component GatACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Part of phosphoenolpyruvate-dependent carbohydrate transport system (PTS). The carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. GatA (IIA domain) interacts with GatB (IIB domain) PMID: 16963640 . Copurifies with trigger factor in pull down assays PMID: 19737520.PMID: 16963640Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFnoyesNo690.109CYT0-0.200913-00NiCytoplasmic1071
PTKB_ECOLIP37188
gatB
b2093 JW2077 cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIB component GatBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544PMID: 16963640 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesYes200.743SpI198.62134-00Yn5-14c19/20oCytoplasmic10
PTHA_ECOLIP05706
srlB

gutB

gutBb2704 JW2673 cytoplasmic peripheral IM protein, PTS system glucitol/sorbitol-specific EIIA component SrlBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)PTS system glucitol/sorbitol-specific EIIA component.The hydropathic profile of the Enzyme IIgut revealed 7 or 8 long hydrophobic segments which may traverse the cell membrane as alpha-helices as well as 2 or 4 short strongly hydrophobic stretches which may traverse the membrane as beta-structure PMID: 3553176.PMID: 2824435 PMID: 3553176Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo350.143CYT0-0.200913-00NiCytoplasmic8115
PTGA_ECOLIP69783
crr

gsr iex tgs treD

gsr iex tgs treDb2417 JW2410 cytoplasmic peripheral IM protein, PTS system glucose-specific EIIA component CrrCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 12717030 PMID: 2960675 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)N/AyesyesNo440.106CYT0-0.200913-00NiCytoplasmic10113
PTNAB_ECOLIP69797
manX

gptB ptsL

gptB ptsLb1817 JW1806 cytoplasmic peripheral IM protein, PTS system mannose-specific EIIAB component ManXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component. Identified by native-PAGE technique for the study of membrane protein complexes in the inner and outer membranes of Escherichia coli. The mannose phosphotransferase system transporter (EIIman) is composed of three subunits, ManX (IIABman), ManY (IICman), and ManZ (IIDman), which are reported to assemble in a 2:1:2 stoichiometry. The IIC and IID components are integral inner membrane proteins. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 10094700 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 15919657 PMID: 15911532 PMID: 10094700 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionnoyesNo430.126CYT0-0.200913-00NiCytoplasmic1093
PTQA_ECOLIP69791
chbA

celC

celCb1736 JW1725 cytoplasmic peripheral IM protein, PTS system N,N'-diacetylchitobiose-specific EIIA component ChbACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)IIA component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC.PMID: 10913122Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/Apseudogene (Ochman & Davalos, 2006)yesyesNo380.187CYT0-0.200913-00NiCytoplasmic10111
PTQB_ECOLIP69795
chbB

celA

celAb1738 JW1727 cytoplasmic peripheral IM protein, PTS system N,N'-diacetylchitobiose-specific EIIB component ChbBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IIB component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC. PMID: 2092358PMID: 10913122 PMID: 2092358Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Membrane associated (Experimental)N/AyesyesNo280.176CYT0-0.200913-00Yn8-19c27/28oCytoplasmic1097
HINT_ECOLIP0ACE7
hinT

ycfF

ycfFb1103 JW1089 cytoplasmic peripheral IM protein, Purine nucleoside phosphoramidase HinTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo570.182CYT0-0.200913-00NiCytoplasmic984
DEOD_ECOLIP0ABP8
deoD

pup

pupb4384 JW4347 cytoplasmic peripheral IM protein, Purine nucleoside phosphorylase DeoD-type DeoDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo270.108CYT0-0.200913-00NiCytoplasmic9108
YCJV_ECOLIP77481
ycjV

ymjB

ymjBb4524 JW1311/JW5203 b1318cytoplasmic peripheral IM protein, Putative ABC transporter ATP-binding YcjV Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/Apseudogene (Uniprot)yesnoNo370.103CYT0-0.200913-00NiCytoplasmicMembrane910
WBBK_ECOLIP37751
wbbK

yefI

yefIb2032 JW2017 cytoplasmic peripheral IM protein, Putative glycosyltransferase WbbK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo210.119CYT0-0.200913-00NiUnknown232
SGRT_ECOLIC1P5Z7
sgrT
b4662 JW0068.1 cytoplasmic peripheral IM protein, Putative inhibitor of glucose uptake transporter SgrT Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Putative inhibitor of glucose uptake transporter sgrT. SgrT protein expresssion is inducted by ??-methylglucoside.PMID: 18042713Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1-N/AnoyesNo230.105CYT0-0.200913-00NiUnknown2
YIDD_ECOLIP0A8C8
yidD
b4557 JW3682 cytoplasmic peripheral IM protein, Putative insertion efficiency factor YidDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)PMID: 21803992Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AnoyesNo420.11CYT0-0.200913-00NiUnknown2
LONH_ECOLIP75867
ycbZ
b0955 JW0938 cytoplasmic peripheral IM protein, Putative Lon protease homolog YcbZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo240.113CYT0-0.200913-00NiCytoplasmic9
YRAR_ECOLIP45469
yraR
b3152 JW5528 cytoplasmic peripheral IM protein, Putative NAD(P)-binding YraRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo320.195CYT0-0.200913-00NiCytoplasmic912
PHNK_ECOLIP16678
phnK
b4097 JW5727 cytoplasmic peripheral IM protein, Putative phosphonates utilization ATP-binding PhnK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo230.117CYT0-0.200913-00NiCytoplasmicMembrane76
YADI_ECOLIP36881
yadI
b0129 JW0125 cytoplasmic peripheral IM protein, Putative phosphotransferase enzyme IIA component YadI Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Putative phosphotransferase enzyme IIA component yadICytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo460.118CYT0-0.200913-00NiCytoplasmic817
SGCB_ECOLIP58035
sgcB
b4565 JW5967 cytoplasmic peripheral IM protein, Putative phosphotransferase enzyme IIB component SgcB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Part of SgcABC complex, a putative PTSPermease. SgcA (Enzyme IIASgc)Is homologous to the IIA domains of the fructose - and mannitol-specific PTSPermeases (fruB and cmtB) which are peripherally accossiated proteins.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo190.218SpI214.43024-00Yn4-11c19/20oCytoplasmic9
WBBJ_ECOLIP37750
wbbJ

yefH

yefHb2033 JW2018 cytoplasmic peripheral IM protein, Putative polysaccharide biosynthesis O-acetyl transferase WbbJ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo240.107CYT0-0.200913-00NiCytoplasmic9
GSPE_ECOLIP45759
gspE

yheG

yheGb3326 JW3288 cytoplasmic peripheral IM protein, Putative type II secretion system E GspECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)ATPase of the Type II secretion system (cryptic in K12) EspL (GspL homolog) protein interacts through its N-terminal cytoplasmic domain with EspL (GspL homolog) proteinPMID: 17255937 PMID: 16525507 PMCID: PMC398259Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo230.111CYT0-0.200913-00NiCytoplasmic921
YEEL_ECOLIP76349
yeeL
b4497 b1979/b1980cytoplasmic peripheral IM protein, Putative YeeLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane Fractionpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoNo190.131CYT0-0.200913-00NiCytoplasmic813
SAPD_ECOLIP0AAH4
sapD

trkE

trkEb1291 JW1284 cytoplasmic peripheral IM protein, Putrescine export system ATP-binding SapD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo530.145CYT0-0.200913-00NiCytoplasmicMembrane722
SAPF_ECOLIP0AAH8
sapF
b1290 JW1283 cytoplasmic peripheral IM protein, Putrescine export system ATP-binding SapF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo450.122CYT0-0.200913-00NiCytoplasmicMembrane78
POTG_ECOLIP31134
potG
b0855 JW5818 cytoplasmic peripheral IM protein, Putrescine transport ATP-binding PotG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo350.11CYT0-0.200913-00NiCytoplasmicMembrane711
PDXH_ECOLIP0AFI7
pdxH
b1638 JW1630 cytoplasmic peripheral IM protein, Pyridoxine/pyridoxamine 5'-phosphate oxidase PdxHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo430.121CYT0-0.200913-00NiCytoplasmic951
ODP1_ECOLIP0AFG8
aceE
b0114 JW0110 cytoplasmic peripheral IM protein, Pyruvate dehydrogenase E1 component AceECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)AceE is the E1 subunit of the 10MDa Escherichia coli pyruvate dehydrogenase multienzyme complex, which catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO(2) and acetate (crystal structure solved as a dimer PMID: 11955070). The E1 subunit is present as a 24mer within the complex. AceE is also part of a very large complex partially resolved by cryoEM PMID: 16338405. Analogous enzyme pyryvate oxidase-PoxB is membrane associated PMID: 18988747. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18988747 PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)TFyesyesNo530.105CYT0-0.200913-00NiCytoplasmic915
POXB_ECOLIP07003
poxB
b0871 JW0855 cytoplasmic peripheral IM protein, Pyruvate dehydrogenase PoxBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Is one of several membrane-associated form of the enzyme has a tendency to self-aggregate,is flavo protein dehydrogenases that feed electrons in to the high lysurfacelabile,and manifests. Pyruvate oxidase is a membrane-associated flavoprotein dehydrogenase which is greatly activated by lipids and detergents. The carboxyl-terminal region of the protein has been shown to play a critical role in the interaction with lipids.PMID: 2663858Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo620.106CYT0-0.200913-00NiCytoplasmicMembrane1020
KPYK1_ECOLIP0AD61
pykF
b1676 JW1666 cytoplasmic peripheral IM protein, Pyruvate kinase I PykFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo420.167CYT0-0.200913-00NiCytoplasmic9108
KPYK2_ECOLIP21599
pykA
b1854 JW1843 cytoplasmic peripheral IM protein, Pyruvate kinase II PykACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo430.131CYT0-0.200913-00NiCytoplasmic943
DLD_ECOLIP06149
dld
b2133 JW2121 cytoplasmic peripheral IM protein, Quinone-dependent D-lactate dehydrogenase DldCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Requires phospholipid for maximal activity. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo330.121CYT0-0.200913-00NiCytoplasmicMembrane1078
RECA_ECOLIP0A7G6
recA

lexB recH rnmB tif umuB zab

lexB recH rnmB tif umuB zabb2699 JW2669 cytoplasmic peripheral IM protein, RecACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544PMID: 2228969Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicSoluble protein detected in Inner Membrane FractionTFnoyesNo30.103CYT0-0.200913-00NiCytoplasmic1088
NARG_ECOLIP09152
narG

bisD narC

bisD narCb1224 JW1215 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 1 alpha chain NarGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. PMID: 12910261 In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. PMID: 19295650 When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. PMID: 2674654 The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. PMID: 3371362 The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. PMID: 16286471 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 12910261 PMID: 16286471 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo200.124CYT0-0.200913-00NiCytoplasmicMembrane1029
NARH_ECOLIP11349
narH
b1225 JW1216 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 1 beta chain NarHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. PMID: 12910261 In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. PMID: 19295650 When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. PMID: 2674654 The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. PMID: 3371362 The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. PMID: 16286471 The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. PMID: 16286471 PMID: 3053688 PMID: 2233673 PMID: 2674654 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 12910261 PMID: 3053688 PMID: 2233673 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo320.117CYT0-0.200913-00NiCytoplasmicMembrane1013
NARZ_ECOLIP19319
narZ
b1468 JW1463 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 2 alpha chain NarZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinCytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo200.145CYT0-0.200913-00NiCytoplasmicMembrane10
NARY_ECOLIP19318
narY
b1467 JW1462 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 2 beta chain NarYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo320.124CYT0-0.200913-00NiCytoplasmicMembrane1014
WBBL_ECOLIP36667
wbbL
b4571 JW2016 b2031cytoplasmic peripheral IM protein, Rhamnosyltransferase WbbL Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo510.111CYT0-0.200913-00NiUnknown254
RHSB_ECOLIP16917
rhsB
b3482 JW5679 cytoplasmic peripheral IM protein, RhsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ARhs ElementnonoNo460.116CYT0-0.200913-00NiExtracellular9
RNE_ECOLIP21513
rne

ams hmp1

ams hmp1b1084 JW1071 cytoplasmic peripheral IM protein, Ribonuclease E RneCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Membrane-binding specificity of NRne was confirmed using circular dichroism difference spectroscopy. RnE binds specifically to anionic phospholipids through electrostatic interactions through N-terminal region (NRne, residues 1-499). Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2011493 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. PMID: 24438330PMID: 18976283 PMID: 15911532 PMID: 22509045 PMID: 2011493 PMID: 23230279 PMID: 24438330Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo620.101CYT0-0.200913-00NiCytoplasmic10
RNR_ECOLIP21499
rnr

vacB yjeC

vacB yjeCb4179 JW5741 cytoplasmic peripheral IM protein, Ribonuclease R RnrCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo30.1CYT0-0.200913-00NiCytoplasmic945
RIR1_ECOLIP00452
nrdA

dnaF

dnaFb2234 JW2228 cytoplasmic peripheral IM protein, Ribonucleoside-diphosphate reductase 1 subunit alpha NrdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490PMID: 23230279 PMID: 9395490Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo400.104CYT0-0.200913-00NiCytoplasmic963
RBSA_ECOLIP04983
rbsA
b3749 JW3728 cytoplasmic peripheral IM protein, Ribose import ATP-binding RbsA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo350.548CYT0-0.200913-00NiCytoplasmicMembrane1049
KPRS_ECOLIP0A717
prs

prsA

prsAb1207 JW1198 cytoplasmic peripheral IM protein, Ribose-phosphate pyrophosphokinase PrsCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo310.131CYT0-0.200913-00NiCytoplasmic109
RLUB_ECOLIP37765
rluB

yciL

yciLb1269 JW1261 cytoplasmic peripheral IM protein, Ribosomal large subunit pseudouridine synthase B RluBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo320.101CYT0-0.200913-00NiCytoplasmic9
RLME_ECOLIP0C0R7
rlmE

ftsJ mrsF rrmJ

ftsJ mrsF rrmJb3179 JW3146 cytoplasmic peripheral IM protein, Ribosomal RNA large subunit methyltransferase E RlmECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 21529161 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo290.111CYT0-0.200913-00NiCytoplasmic916
RLMI_ECOLIP75876
rlmI

yccW

yccWb0967 JW5898 cytoplasmic peripheral IM protein, Ribosomal RNA large subunit methyltransferase I RlmICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo290.117CYT0-0.200913-00NiCytoplasmic9
RSMA_ECOLIP06992
rsmA

ksgA

ksgAb0051 JW0050 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase A RsmACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; Overex<x>pression of RsmA suppresses defects in mutant of era. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 21529161 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo330.128CYT0-0.200913-00NiCytoplasmic10
RSMC_ECOLIP39406
rsmC

yjjT

yjjTb4371 JW4333 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase C RsmCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Ribosom relatedPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo270.158CYT0-0.200913-00NiCytoplasmic943
RSME_ECOLIP0AGL7
rsmE

yggJ

yggJb2946 JW2913 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase E RsmECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)RibosomalPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicIM (predicted)yesyesNo400.129CYT0-0.200913-00NiCytoplasmic818
RSMH_ECOLIP60390
rsmH

mraW yabC

mraW yabCb0082 JW0080 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase H RsmHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 10572301 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 10572301 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo540.11CYT0-0.200913-00NiCytoplasmic1065
RIMJ_ECOLIP0A948
rimJ
b1066 JW1053 cytoplasmic peripheral IM protein, Ribosomal-protein-alanine acetyltransferase RimJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associate with the Pre-30S subunit. Overex<x>pression of RimJ suppresses defects in S5(G28D) mutations.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo330.107CYT0-0.200913-00NiCytoplasmic10
RIMP_ECOLIP0A8A8
rimP

yhbC

yhbCb3170 JW5533 cytoplasmic peripheral IM protein, Ribosome maturation factor RimP Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm ( Experimental)Assembly cofactor of ribosome; associate with the 30S subunit.PMID: 21529161Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo190.107CYT0-0.200913-00NiCytoplasmic9111
YFIA_ECOLIP0AD49
raiA

yfiA

yfiAb2597 JW2578 cytoplasmic peripheral IM protein, Ribosome-associated inhibitor A RaiACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)TranslationPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo630.108CYT0-0.200913-00NiCytoplasmic1098
RRF_ECOLIP0A805
frr

rrf

rrfb0172 JW0167 cytoplasmic peripheral IM protein, Ribosome-recycling factor FrrCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo330.103CYT0-0.200913-00NiCytoplasmic10109
PROQ_ECOLIP45577
proQ

yebJ yobE yoeC

yebJ yobE yoeCb1831 JW5300 cytoplasmic peripheral IM protein, RNA chaperone Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo270.162CYT0-0.200913-00NiCytoplasmic869
RAPZ_ECOLIP0A894
rapZ

yhbJ

yhbJb3205 JW3172 cytoplasmic peripheral IM protein, RNase adapter RapZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo180.112CYT0-0.200913-00NiUnknown738
FRMA_ECOLIP25437
frmA

adhC

adhCb0356 JW0347 cytoplasmic peripheral IM protein, S-(hydroxymethyl)glutathione dehydrogenase FrmACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicIM (predicted)yesyesNo500.109CYT0-0.200913-00NiCytoplasmic1071
METK_ECOLIP0A817
metK

metX

metXb2942 JW2909 cytoplasmic peripheral IM protein, S-adenosylmethionine synthase MetKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of MetK have been monitored via fluorescence microscopy. Cells containing almost exclusively diffuse cytoplasmic MetK, or focused locations.PMID: 17272300 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo640.118CYT0-0.200913-00NiCytoplasmic1047
LUXS_ECOLIP45578
luxS

ygaG

ygaGb2687 JW2662 cytoplasmic peripheral IM protein, S-ribosylhomocysteine lyase LuxSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFnoyesNo190.106CYT0-0.200913-00NiCytoplasmic8110
MIND_ECOLIP0AEZ3
minD
b1175 JW1164 cytoplasmic peripheral IM protein, Septum site-determining MinD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 10096083 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo250.128CYT0-0.200913-00NiCytoplasmicMembrane1095
GLYA_ECOLIP0A825
glyA
b2551 JW2535 cytoplasmic peripheral IM protein, Serine hydroxymethyltransferase Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AyesyesNo470.123CYT0-0.200913-00NiCytoplasmic10
SYS_ECOLIP0A8L1
serS
b0893 JW0876 cytoplasmic peripheral IM protein, Serine--tRNA ligase SerSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unitPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo670.1CYT0-0.200913-00NiCytoplasmic1028
SRP54_ECOLIP0AGD7
ffh
b2610 JW5414 cytoplasmic peripheral IM protein, Signal recognition particle FfhCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18281057 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo630.1CYT0-0.200913-00NiUnknown5106
FTSY_ECOLIP10121
ftsY
b3464 JW3429 cytoplasmic peripheral IM protein, Signal recognition particle receptor FtsY Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains an essential and autonomous membrane-binding amphipathic helix. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 18281057 PMID: 8194520 PMID: 9177162 PMID: 11353766 PMID: 17726013 PMID: 19414018 PMID: 21543314 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo30.107CYT0-0.200913-00NiCytoplasmicMembrane10
UGPC_ECOLIP10907
ugpC
b3450 JW3415 cytoplasmic peripheral IM protein, sn-glycerol-3-phosphate import ATP-binding UgpC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo370.105CYT0-0.200913-00NiCytoplasmicMembrane1020
STHA_ECOLIP27306
sthA

sth udhA

sth udhAb3962 JW5551 cytoplasmic peripheral IM protein, Soluble pyridine nucleotide transhydrogenase SthACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SthA is bound on the inner mebrane through PntAB heterodimer. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 14660605 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/ATFyesyesNo220.133CYT0-0.200913-00NiCytoplasmic1022
POTA_ECOLIP69874
potA
b1126 JW1112 cytoplasmic peripheral IM protein, Spermidine/putrescine import ATP-binding PotA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 1939142 PMID: 7592703 PMID: 11976340 PMID: 8366082 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo220.124CYT0-0.200913-00NiCytoplasmicMembrane993
SRA_ECOLIP68191
sra

rpsV

rpsVb1480 JW5962 cytoplasmic peripheral IM protein, Stationary-phase-induced ribosome-associated SraCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Small protein identified experimentallyPMID: 19121005. Stationary-phase-induced and ribosome-associated proteinPMID: 11292794. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry)PMID: 23230279.PMID: 11292794 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AnoyesNo260.1CYT0-0.200913-00NiCytoplasmic10
SSPB_ECOLIP0AFZ3
sspB
b3228 JW3197 cytoplasmic peripheral IM protein, Stringent starvation B SspBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo470.112CYT0-0.200913-00NiCytoplasmic8
SDHA_ECOLIP0AC41
sdhA
b0723 JW0713 cytoplasmic peripheral IM protein, Succinate dehydrogenase flavosubunit SdhACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 9600841 PMID: 9740056 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 12560550 PMID: 9600841 PMID: 9740056 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionTFyesyesNo500.189CYT0-0.200913-00NiCytoplasmicMembrane1010
SDHB_ECOLIP07014
sdhB
b0724 JW0714 cytoplasmic peripheral IM protein, Succinate dehydrogenase iron-sulfur subunit SdhBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 6388571 PMID: 2987185 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 16079137 PMID: 6388571 PMID: 2987185 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Cytoplasmic (Experimental)IM (predicted)yesyesNo280.102CYT0-0.200913-00NiCytoplasmicMembrane1034
SUCD_ECOLIP0AGE9
sucD
b0729 JW0718 cytoplasmic peripheral IM protein, Succinate--CoA ligase (ADP-forming) subunit alpha SucDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo310.171CYT0-0.200913-00NiCytoplasmic970
SUCC_ECOLIP0A836
sucC
b0728 JW0717 cytoplasmic peripheral IM protein, Succinate--CoA ligase SucCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo320.181CYT0-0.200913-00NiCytoplasmic867
GABD_ECOLIP25526
gabD
b2661 JW2636 cytoplasmic peripheral IM protein, Succinate-semialdehyde dehydrogenase GabDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo280.124CYT0-0.200913-00NiCytoplasmic960
SUFA_ECOLIP77667
sufA

ydiC

ydiCb1684 JW1674 cytoplasmic peripheral IM protein, SufACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)PMID: 19366265Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesnoNo340.137CYT0-0.200913-00NiCytoplasmic1085
CYSA_ECOLIP16676
cysA
b2422 JW2415 cytoplasmic peripheral IM protein, Sulfate/thiosulfate import ATP-binding CysA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo330.112CYT0-0.200913-00NiCytoplasmicMembrane714
SYDP_ECOLIP0A8U0
syd

ydr

ydrb2793 JW2764 cytoplasmic peripheral IM protein, SydCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein; Cytoplasmic side CytoplasmicN/AyesyesNo200.125CYT0-0.200913-00NiCytoplasmicMembrane1059
DMSD_ECOLIP69853
dmsD

ynfI

ynfIb1591 JW5262 cytoplasmic peripheral IM protein, Tat proofreading chaperone DmsD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Chaperone for TAT signal peptides The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592 DmsD is a redox enzyme maturation protein (REMP) which interacts with the twin-arginine leader sequence of DmsA, the catalytic subunit of DMSO reductase. It interacts with several general chaperones (DnaK, DnaJ, GrpE, GroEL, Tig and Ef-Tu) via in vitro protein-protein interaction screening approaches. It was also found to interact with proteins involved in the molybdenum cofactor biosynthesis pathway. PMID: 20153451PMID: 19652330 PMID: 12813051 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo310.453SpI302.43679-00NiCytoplasmicMembrane10
TAUB_ECOLIQ47538
tauB

ssiB yaiQ

ssiB yaiQb0366 JW0358 cytoplasmic peripheral IM protein, Taurine import ATP-binding TauB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo570.124CYT0-0.200913-00NiCytoplasmicMembrane1010
WECF_ECOLIP56258
wecF

rffT yifM

rffT yifMb4481 JW5596 cytoplasmic peripheral IM protein, TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase WecFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1in/inCell inner membrane CytoplasmicIM (predicted)pseudogene (Ochman & Davalos, 2006)yesyesNo290.203CYT0-0.200913-00NiUnknown248
THIQ_ECOLIP31548
thiQ

yabJ

yabJb0066 JW0065 cytoplasmic peripheral IM protein, Thiamine import ATP-binding ThiQ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Two very weak TM predictions; membrane associated activity known. Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system PMID: 12175925PMID: 12175925Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo250.144CYT0-0.200913-00NiCytoplasmicMembrane108
THIK_ECOLIP75948
thiK

ycfN

ycfNb1106 JW1092 cytoplasmic peripheral IM protein, Thiamine kinase ThiKCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo390.11CYT0-0.200913-00NiUnknown29
TPX_ECOLIP0A862
tpx

yzzJ

yzzJb1324 JW1317 cytoplasmic peripheral IM protein, Thiol peroxidase TpxCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. PMID: 7499381 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 7499381 PMID: 9298646Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Periplasm Periplasmic (Experimental)N/AyesyesNo330.123CYT0-0.200913-00NiPeriplasmic10
THIO_ECOLIP0AA25
trxA

fipA tsnC

fipA tsnCb3781 JW5856 cytoplasmic peripheral IM protein, Thioredoxin 1 TrxACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bridge (33 36) PMID: 10489448 Disulfide bond-containing protein PMID: 25664544PMID: 23230279 PMID: 10489448Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFnoyesNo200.119CYT0-0.200913-00NiCytoplasmic989
SYT_ECOLIP0A8M3
thrS
b1719 JW1709 cytoplasmic peripheral IM protein, Threonine--tRNA ligase ThrSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo330.154CYT0-0.200913-00NiCytoplasmic1022
TYPH_ECOLIP07650
deoA

tpp ttg

tpp ttgb4382 JW4345 cytoplasmic peripheral IM protein, Thymidine phosphorylase DeoACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)noyesNo530.104CYT0-0.200913-00NiCytoplasmic812
TALB_ECOLIP0A870
talB

yaaK

yaaKb0008 JW0007 cytoplasmic peripheral IM protein, Transaldolase B TalBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo330.122CYT0-0.200913-00NiCytoplasmic884
RHO_ECOLIP0AG30
rho

nitA psuA rnsC sbaA tsu

nitA psuA rnsC sbaA tsub3783 JW3756 cytoplasmic peripheral IM protein, Transcription termination factor Rho Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo440.103CYT0-0.200913-00NiCytoplasmic9
NUSG_ECOLIP0AFG0
nusG
b3982 JW3945 cytoplasmic peripheral IM protein, Transcription termination/antitermination NusG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo260.108CYT0-0.200913-00NiCytoplasmic972
TKT1_ECOLIP27302
tktA

tkt

tktb2935 JW5478 cytoplasmic peripheral IM protein, Transketolase 1 TktACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFyesyesNo250.124CYT0-0.200913-00NiCytoplasmic932
TKT2_ECOLIP33570
tktB
b2465 JW2449 cytoplasmic peripheral IM protein, Transketolase 2 TktBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo240.145CYT0-0.200913-00NiCytoplasmic911
SECA_ECOLIP10408
secA

azi pea prlD

azi pea prlDb0098 JW0096 cytoplasmic peripheral IM protein, translocase subunit SecA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15919657 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo700.109CYT0-0.200913-00NiCytoplasmicMembrane1058
INSH5_ECOLIP76071
insH5
b1370 JW1363 cytoplasmic peripheral IM protein, Transposase InsH for insertion sequence element IS5Y Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionInsertion Sequence, Transposase, pseudogene (Uniprot)nonoNo490.125CYT0-0.200913-00NiCytoplasmic8
TREC_ECOLIP28904
treC

olgH

olgHb4239 JW4198 cytoplasmic peripheral IM protein, Trehalose-6-phosphate hydrolase TreCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/ATFyesyesNo600.145CYT0-0.200913-00NiCytoplasmic109
OTSA_ECOLIP31677
otsA
b1896 JW5312 cytoplasmic peripheral IM protein, Trehalose-6-phosphate synthase OtsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo190.248CYT0-0.200913-00NiCytoplasmic1030
TIG_ECOLIP0A850
tig
b0436 JW0426 cytoplasmic peripheral IM protein, Trigger factor TigCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit PMID: 8633085 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 23230279 PMID: 8633085 PMID: 20153451Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo210.128CYT0-0.200913-00NiCytoplasmic887
TPIS_ECOLIP0A858
tpiA

tpi

tpib3919 JW3890 cytoplasmic peripheral IM protein, Triosephosphate isomerase TpiACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo400.118CYT0-0.200913-00NiCytoplasmic996
TRKA_ECOLIP0AGI8
trkA
b3290 JW3251 cytoplasmic peripheral IM protein, Trk system potassium uptake TrkA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 2674131 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionnoyesNo170.13CYT0-0.200913-00Yn3-11c16/17oCytoplasmicMembrane1068
TRMJ_ECOLIP0AE01
trmJ

yfhQ

yfhQb2532 JW2516 cytoplasmic peripheral IM protein, tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo630.12CYT0-0.200913-00NiCytoplasmic978
MNME_ECOLIP25522
mnmE

thdF trmE

thdF trmEb3706 JW3684 cytoplasmic peripheral IM protein, tRNA modification GTPase MnmE Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)A GTPase that exhibits high intrinsic GTPase hydrolysis rate,Low affinity for GTP, and extremely low affinity for GDP Can form self-assemblies. Is a cytoplasmic protein partially associated with the inner membrane. Involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs. Essential for viability depending on genetic background. Fluorescent fusions of MnmE have been monitored via fluorescence microscopy. MnmE located predominantly beside the membrane.PMID: 10601028 PMID: 17272300Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo470.153CYT0-0.200913-00NiCytoplasmic989
MNMG_ECOLIP0A6U3
mnmG

gidA trmF

gidA trmFb3741 JW3719 cytoplasmic peripheral IM protein, tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Similar localization to mnmE; may form a complex together PMID: 17062623Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo250.172CYT0-0.200913-01No601-619iCytoplasmic96
DUSA_ECOLIP32695
dusA

yjbN

yjbNb4049 JW5950 cytoplasmic peripheral IM protein, tRNA-dihydrouridine(20/20a) synthase DusACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.108CYT0-0.200913-00NiCytoplasmic9
YFIF_ECOLIP0AGJ5
yfiF
b2581 JW2565 cytoplasmic peripheral IM protein, tRNA/rRNA methyltransferase YfiF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/ATFnoyesNo450.103CYT0-0.200913-00NiCytoplasmic981
SYY_ECOLIP0AGJ9
tyrS
b1637 JW1629 cytoplasmic peripheral IM protein, Tyrosine--tRNA ligase TyrSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unitPMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmicN/AnoyesNo650.123CYT0-0.200913-00NiCytoplasmic1063
LPXH_ECOLIP43341
lpxH

ybbF

ybbFb0524 JW0513 cytoplasmic peripheral IM protein, UDP-2,3-diacylglucosamine hydrolase LpxHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phobius weak prediction of a hydrophobic TMIn the middle of sequence/Kyte-Doolittle predicts several hydrophobic patches PMID: 20608695Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo330.16CYT0-0.200913-00NiCytoplasmic1020
WECG_ECOLIP27836
wecG

rffM

rffMb3794 JW3770 cytoplasmic peripheral IM protein, UDP-N-acetyl-D-mannosaminuronic acid transferase WecGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo270.16CYT0-0.200913-00NiCytoplasmic835
MURG_ECOLIP17443
murG
b0090 JW0088 cytoplasmic peripheral IM protein, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase MurGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Pyrophosphoryl-undecaprenol N-acetylglucosamine transferase. Murein synthesis peripheral membrane protein interacting with cardiolipin. PMID: 8449890 PMID: 10892798 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 8449890 PMID: 10892798 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane Membrane associated (Experimental)N/AyesyesNo270.156TMH03.61924-00NiCytoplasmicMembrane1048
MURC_ECOLIP17952
murC
b0091 JW0089 cytoplasmic peripheral IM protein, UDP-N-acetylmuramate--L-alanine ligase MurCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble IM Fraction +TFyesyesNo410.228CYT0-0.200913-00NiCytoplasmic976
USPA_ECOLIP0AED0
uspA
b3495 JW3462 cytoplasmic peripheral IM protein, Universal stress A UspACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)The Usp superfamily encompasses an ancient and conserved group of proteins that are found in bacteria, archaea,fungi, flies and plants. Usually each organism has several copies of Usp genes. Escherichia coli contains six Usp proteins, which can be divided into two subfamilies on the basis of sequence similarity. PMID: 19101563 Three of these proteins belong to the UspA subfamily (UspA, UspC, and UspD), and two to the UspFG subfamily (UspF and UspG). Another homologue is YbdQ. UspC binds to the membrane-integrated sensor kinase KdpD involved in K+ ion sensing. The same could be true for the other Usp proteins but we could not find direct evidence in the literature. PMID: 1453957 PMID: 8506346 PMID: 8740179 PMID: 9405142 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 15911532 PMID: 19101563 PMID: 1453957 PMID: 8506346 PMID: 8740179 PMID: 9405142 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmCytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo330.153CYT0-0.200913-00NiCytoplasmic1080
USPD_ECOLIP0AAB8
uspD

yiiT

yiiTb3923 JW3894 cytoplasmic peripheral IM protein, Universal stress D UspDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo540.108CYT0-0.200913-00NiCytoplasmic996
USPG_ECOLIP39177
uspG

ybdQ yzzU

ybdQ yzzUb0607 JW0600 cytoplasmic peripheral IM protein, Universal stress UP12 UspGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 8740179 PMID: 9600841 PMID: 12071968 PMID: 16460009 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 8740179 PMID: 9600841 PMID: 12071968 PMID: 16460009 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasmic (Experimental)Soluble protein detected in Inner Membrane FractionyesyesNo530.335CYT0-0.200913-00NiCytoplasmic885
YJGA_ECOLIP0A8X0
yjgA
b4234 JW4193 cytoplasmic peripheral IM protein, UPF0307 YjgACytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal (Experimental)PMID: 23230279 PMID: 16980477Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rA,F1,rCytoplasm CytoplasmicN/AyesyesNo460.101CYT0-0.200913-00NiCytoplasmic981
YAEH_ECOLIP62768
yaeH
b0163 JW0159 cytoplasmic peripheral IM protein, UPF0325 YaeHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo260.108CYT0-0.200913-00NiCytoplasmic893
YIFE_ECOLIP0ADN2
yifE
b3764 JW3737 cytoplasmic peripheral IM protein, UPF0438 YifECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo440.105CYT0-0.200913-00NiUnknown2
YGIB_ECOLIP0ADT2
ygiB
b3037 JW5927 cytoplasmic peripheral IM protein, UPF0441 YgiBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein PMID: 25664544PMID: 20545840 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo360.178SpII369.72422Pos+2=E00Yn21-32c37/38oPeriplasmic9
PYRH_ECOLIP0A7E9
pyrH

smbA

smbAb0171 JW0166 cytoplasmic peripheral IM protein, Uridylate kinase PyrHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of PyrH have been monitored via fluorescence microscopy. PyrH was found to be located in one membrane-associated foci per cell. PMID: 17272300 PyrH-EYFP fusions are predominantly localized near the bacterial membranes of different cells undergoing septation and division. PMID: 9922246 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 9922246 PMID: 17272300 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicN/AyesyesNo230.14CYT0-0.200913-00NiCytoplasmic930
UVRA_ECOLIP0A698
uvrA

dinE

dinEb4058 JW4019 cytoplasmic peripheral IM protein, UvrABC system A UvrACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cytoplasm CytoplasmicSoluble protein detected in Inner Membrane FractionTFnoyesNo420.254CYT0-0.200913-00NiCytoplasmic9
BTUD_ECOLIP06611
btuD
b1709 JW1699 cytoplasmic peripheral IM protein, Vitamin B12 import ATP-binding BtuD Vitamin B12 import ATP-binding protein btuD.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane; Peripheral membrane proteinCytoplasmicN/AyesyesNo260.195CYT0-0.200913-00NiCytoplasmicMembrane109
XGPT_ECOLIP0A9M5
gpt

gpp gxu

gpp gxub0238 JW0228 cytoplasmic peripheral IM protein, Xanthine phosphoribosyltransferase GptCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo480.21CYT0-0.200913-00NiCytoplasmic10
XYLG_ECOLIP37388
xylG
b3567 JW3539 cytoplasmic peripheral IM protein, Xylose import ATP-binding XylG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicN/AyesyesNo200.188CYT0-0.200913-00NiCytoplasmicMembrane748
YAAY_ECOLIP75620
yaaY
b0024 JW5003 cytoplasmic peripheral IM protein, YaaYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Membrane anchoredN/AnoyesNo430.125CYT0-0.200913-10NiCytoplasmicMembrane9
YAFS_ECOLIP75672
yafS
b0213 JW0203 cytoplasmic peripheral IM protein, YafSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo540.121CYT0-0.200913-00NiCytoplasmic8
YAGA_ECOLIP37007
yagA
b0267 JW0260 cytoplasmic peripheral IM protein, YagACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionProphage Integrase (EcoGene)nonoNo290.125CYT0-0.200913-00NiCytoplasmic82
YAHF_ECOLIP77187
yahF
b0320 JW0312 cytoplasmic peripheral IM protein, YahFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo280.139CYT0-0.200913-00NiCytoplasmic8
YBJS_ECOLIP75821
ybjS
b0868 JW5819 cytoplasmic peripheral IM protein, YbjSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo300.118CYT0-0.200913-00NiCytoplasmic86
YBJX_ECOLIP75829
ybjX
b0877 JW0861 cytoplasmic peripheral IM protein, YbjXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)In Shigella the homologous protein is required for the expression, or correct membrane localisation of IcsA (VirG) on the bacterial cell surface.In Pasteurella haemolytica lapB (P32181), which is thought to be membrane-associated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo200.111CYT0-0.200913-00NiUnknown221
YCAK_ECOLIP43340
ycaK
b0901 JW0884 cytoplasmic peripheral IM protein, YcaKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)noyesNo230.238CYT0-0.200913-00NiCytoplasmic995
YDIJ_ECOLIP77748
ydiJ
b1687 JW1677 cytoplasmic peripheral IM protein, YdiJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279 PMID: 15919657Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo290.119CYT0-0.200913-00NiCytoplasmic92
YEDJ_ECOLIP46144
yedJ
b1962 JW1945 cytoplasmic peripheral IM protein, YedJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)noyesNo650.139CYT0-0.200913-00NiCytoplasmic8116
YFFO_ECOLIP76546
yffO
b2446 cytoplasmic peripheral IM protein, YffOCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionProphage Integrase (EcoGene)nonoNo610.105CYT0-0.200913-00NiCytoplasmic8
YFFR_ECOLIP76549
yffR
b2449 cytoplasmic peripheral IM protein, YffRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionProphage Integrase (EcoGene)nonoNo200.169CYT0-0.200913-00Yn3-14c19/20oUnknown2
YFJP_ECOLIP52131
yfjP
b2632 JW5419 cytoplasmic peripheral IM protein, YfjPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Fluorescent fusions of YfjP have been monitored via fluorescence microscopy. YfjP located predominantly beside the membrane.PMID: 17272300Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AProphage Integrase (EcoGene)nonoNo590.126CYT0-0.200913-00NiUnknown235
YGEH_ECOLIP76639
ygeH
b2852 JW2820 cytoplasmic peripheral IM protein, YgeHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Fluorescent fusions of YgeH have been monitored via fluorescence microscopy. YgeH located predominantly beside the membrane.PMID: 17272300Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AnoyesNo530.108CYT0-0.200913-00NiCytoplasmic819
YGGL_ECOLIP38521
yggL
b2959 JW2926 cytoplasmic peripheral IM protein, YggLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicN/AyesyesNo290.118CYT0-0.200913-00NiCytoplasmic8129
YHGF_ECOLIP46837
yhgF
b3407 JW3370 cytoplasmic peripheral IM protein, YhgFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo240.217CYT0-0.200913-00NiCytoplasmic990
YIEE_ECOLIP0ADM8
yieE
b3712 JW3690 cytoplasmic peripheral IM protein, YieECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)yesyesNo250.109CYT0-0.200913-00NiUnknown27
YIGI_ECOLIP0ADP2
yigI
b3820 JW5588 cytoplasmic peripheral IM protein, YigICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 19766000 PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected), Soluble IM Fraction +yesyesNo280.137CYT0-0.200913-00NiUnknown29
YJFM_ECOLIP39295
yjfM
b4185 JW4143 cytoplasmic peripheral IM protein, YjfMCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC.PMID: 20545840Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)noyesNo550.107CYT0-0.200913-00NiUnknown2105
YJGR_ECOLIP39342
yjgR
b4263 JW4220 cytoplasmic peripheral IM protein, YjgRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted)yesyesNo580.139CYT0-0.200913-00NiUnknown287
YLBE_ECOLIP77129
ylbE
b4572 b0519cytoplasmic peripheral IM protein, YlbECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane Fractionpseudogene (Ochman & Davalos, 2006)noyesNo460.139CYT0-0.200913-00NiCytoplasmic833
YMFJ_ECOLIP75973
ymfJ
b1144 JW1130 cytoplasmic peripheral IM protein, YmfJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.PMID: 19766000Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicSoluble protein detected in Inner Membrane FractionProphage Integrase (EcoGene)nonoNo480.139CYT0-0.200913-00NiCytoplasmic847
YOBI_ECOLIC1P604
yobI
b4677 JW1850.1 cytoplasmic peripheral IM protein, YobISmall membrane protein detected experimentallyCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1-N/AnoyesNo120.1CYT0-0.200913-00NiUnknown2
YSHB_ECOLIC1P620
yshB
b4686 JW3839.1 cytoplasmic peripheral IM protein, YshBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Amphipaseek predicts N-terminal amphipathic helix (1-10). Amphipaseek predicts N-terminal amphipathic helix (1-10). Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1-N/AnoyesNo250.194CYT0-0.200913-00NiUnknown2
ZNUC_ECOLIP0A9X1
znuC

yebM

yebMb1858 JW1847 cytoplasmic peripheral IM protein, Zinc import ATP-binding ZnuC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279PMID: 23230279Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1Cell inner membrane CytoplasmicSoluble protein detected in Inner Membrane FractionyesyesNo220.118CYT0-0.200913-00NiCytoplasmicMembrane715
YDJJ_ECOLIP77280
ydjJ
b1774 JW1763 cytoplasmic peripheral IM protein, zinc-type alcohol dehydrogenase-like YdjJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1A,F1CytoplasmicIM (predicted-detected)noyesNo460.11CYT0-0.200913-00NiCytoplasmic977
PTRB_ECOLIP24555
ptrB

tlp

tlpb1845 JW1834 cytoplasmic protease 2 PtrBCytoplasmicAACytoplasmicN/AnoyesNo170.103CYT0-0.200913-00NiUnknown51
ELAD_ECOLIQ47013
elaD
b2269 JW5840 cytoplasmic protease ElaDCytoplasmicAACytoplasmicN/AnoyesNo230.129CYT0-0.200913-00NiCytoplasmic835
YEGQ_ECOLIP76403
yegQ
b2081 JW2066 cytoplasmic protease YegQCytoplasmicAACytoplasmicN/AnoyesNo220.362CYT0-0.200913-00NiCytoplasmic874
YHBU_ECOLIP45527
yhbU
b3158 JW3127 cytoplasmic protease YhbUCytoplasmicAACytoplasmicN/AyesyesNo240.131CYT0-0.200913-00NiCytoplasmic89
HSCA_ECOLIP0A6Z1
hscA

hsc

hscb2526 JW2510 cytoplasmic protein of Hsp70 family, Chaperone HscACytoplasmicAACytoplasmicN/AyesyesNo220.165CYT0-0.200913-00NiCytoplasmic9101
HSCC_ECOLIP77319
hscC

ybeW

ybeWb0650 JW0645 cytoplasmic protein of Hsp70 family, Chaperone HscCCytoplasmicAACytoplasmicN/AnoyesNo210.135CYT0-0.200913-00NiCytoplasmic974
YEGD_ECOLIP36928
yegD
b2069 JW2054 cytoplasmic protein of Hsp70 family, Chaperone YegDCytoplasmicAACytoplasmicN/AyesyesNo460.153CYT0-0.200913-00NiCytoplasmic98
LSRG_ECOLIP64461
lsrG

yneC

yneCb1518 JW1511 cytoplasmic protein, (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase LsrGCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo330.101CYT0-0.200913-00NiUnknown276
CITC_ECOLIP77390
citC

ybeO

ybeOb0618 JW0610 cytoplasmic protein, (Citrate (pro-3S)-lyase) ligase CitCCytoplasmicAACytoplasmicN/AnoyesNo640.104CYT0-0.200913-00NiCytoplasmic884
PAAG_ECOLIP77467
paaG

ydbT

ydbTb1394 JW1389 cytoplasmic protein, 1,2-epoxyphenylacetyl-CoA isomerase PaaGCytoplasmicAACytoplasmicN/AnoyesNo620.113CYT0-0.200913-00NiCytoplasmic998
PAAA_ECOLIP76077
paaA

ydbO

ydbOb1388 JW1383 cytoplasmic protein, 1,2-phenylacetyl-CoA epoxidase, subunit A PaaACytoplasmicAACytoplasmicN/AnoyesNo290.102CYT0-0.200913-00NiCytoplasmic8
PAAB_ECOLIP76078
paaB

ynbF

ynbFb1389 JW1384 cytoplasmic protein, 1,2-phenylacetyl-CoA epoxidase, subunit B PaaBCytoplasmicAACytoplasmicN/AnoyesNo300.214CYT0-0.200913-00NiUnknown286
PAAE_ECOLIP76081
paaE

ydbR

ydbRb1392 JW1387 cytoplasmic protein, 1,2-phenylacetyl-CoA epoxidase, subunit E PaaECytoplasmicAACytoplasmicN/AnoyesNo260.113CYT0-0.200913-00NiCytoplasmic921
GLGB_ECOLIP07762
glgB
b3432 JW3395 cytoplasmic protein, 1,4-alpha-glucan branching enzyme GlgBCytoplasmicAACytoplasmicN/AyesyesNo270.139CYT0-0.200913-00NiCytoplasmic918
MENI_ECOLIP77781
menI

ydiI

ydiIb1686 JW1676 cytoplasmic protein, 1,4-dihydroxy-2-naphthoyl-CoA hydrolase MenICytoplasmicAACytoplasmicN/AyesyesNo630.118CYT0-0.200913-00NiCytoplasmic878
MENB_ECOLIP0ABU0
menB
b2262 JW2257 cytoplasmic protein, 1,4-dihydroxy-2-naphthoyl-CoA synthase MenBCytoplasmicAACytoplasmicN/AyesyesNo620.107CYT0-0.200913-00NiCytoplasmic971
AMPD_ECOLIP13016
ampD
b0110 JW0106 cytoplasmic protein, 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpDCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo450.136CYT0-0.200913-00NiCytoplasmic1060
HIS4_ECOLIP10371
hisA
b2024 JW2006 cytoplasmic protein, 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino) imidazole-4-carboxamide isomerase HisACytoplasmicAACytoplasmCytoplasmicN/AyesyesNo550.11CYT0-0.200913-00NiCytoplasmic1044
DXR_ECOLIP45568
dxr

ispC yaeM

ispC yaeMb0173 JW0168 cytoplasmic protein, 1-deoxy-D-xylulose 5-phosphate reductoisomerase DxrNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo550.115CYT0-0.200913-00NiCytoplasmicMembrane9
YAJO_ECOLIP77735
yajO
b0419 JW0409 cytoplasmic protein, 1-deoxyxylulose-5-phosphate synthase YajOCytoplasmicAACytoplasmicN/AyesyesNo330.138CYT0-0.200913-00NiCytoplasmic94
K1PF_ECOLIP0AEW9
fruK

fpk

fpkb2168 JW2155 cytoplasmic protein, 1-phosphofructokinase FruKCytoplasmicAACytoplasmicN/AnoyesNo390.121CYT0-0.200913-00NiCytoplasmic979
GPMI_ECOLIP37689
gpmI

pgmI yibO

pgmI yibOb3612 JW3587 cytoplasmic protein, 2,3-bisphosphoglycerate-independent phosphoglycerate mutase GpmICytoplasmicAACytoplasmicN/AyesyesNo200.111CYT0-0.200913-00NiCytoplasmic10104
PAAF_ECOLIP76082
paaF

ydbR

ydbRb1393 JW1388 cytoplasmic protein, 2,3-dehydroadipyl-CoA hydratase PaaFCytoplasmicAACytoplasmicN/AnoyesNo410.148CYT0-0.200913-00NiCytoplasmic971
ENTA_ECOLIP15047
entA
b0596 JW0588 cytoplasmic protein, 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase EntACytoplasmicAACytoplasmicN/AyesyesNo310.19CYT0-0.200913-00NiCytoplasmic963
MHPB_ECOLIP0ABR9
mhpB
b0348 JW0339 cytoplasmic protein, 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase MhpBCytoplasmicAACytoplasmicN/AnoyesNo230.132CYT0-0.200913-00NiCytoplasmicMembrane98
DLGD_ECOLIP37672
dlgD

yiaK

yiaKb3575 JW3547 cytoplasmic protein, 2,3-diketo-L-gulonate reductase DlgDCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo290.127CYT0-0.200913-00NiCytoplasmic936
FADH_ECOLIP42593
fadH

ygjL

ygjLb3081 JW3052 cytoplasmic protein, 2,4-dienoyl-CoA reductase FadHCytoplasmicAACytoplasmicN/AyesyesNo200.11CYT0-0.200913-00NiCytoplasmic917
DKGA_ECOLIQ46857
dkgA

yqhE

yqhEb3012 JW5499 cytoplasmic protein, 2,5-diketo-D-gluconic acid reductase A DkgACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo260.118CYT0-0.200913-00NiCytoplasmic927
DKGB_ECOLIP30863
dkgB

yafB

yafBb0207 JW0197 cytoplasmic protein, 2,5-diketo-D-gluconic acid reductase B DkgBCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo370.144CYT0-0.200913-00NiCytoplasmic978
CITG_ECOLIP77231
citG

ybdT

ybdTb0613 JW0605 cytoplasmic protein, 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CitGCytoplasmicAACytoplasmicIM (predicted)yesyesNo650.116CYT0-0.200913-00NiUnknown25
HPPK_ECOLIP26281
folK
b0142 JW0138 cytoplasmic protein, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase FolKCytoplasmicAACytoplasmicN/AyesyesNo220.266CYT0-0.200913-00NiCytoplasmic966
ISPF_ECOLIP62617
ispF

mecS ygbB

mecS ygbBb2746 JW2716 cytoplasmic protein, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase IspFCytoplasmicAACytoplasmicN/AyesyesNo120.113CYT0-0.200913-00NiCytoplasmic984
ISPD_ECOLIQ46893
ispD

ygbP

ygbPb2747 JW2717 cytoplasmic protein, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase IspDCopurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmicN/ATFyesyesNo470.108CYT0-0.200913-00NiCytoplasmic967
DGOA_ECOLIQ6BF16
dgoA

yidU

yidUb4477 JW5628 cytoplasmic protein, 2-dehydro-3-deoxy-6-phosphogalactonate aldolase DgoACytoplasmicAACytoplasmicN/AnoyesNo240.37CYT0-0.200913-00NiCytoplasmic931
KDUD_ECOLIP37769
kduD

ygeC yqeD

ygeC yqeDb2842 JW2810 cytoplasmic protein, 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduDCytoplasmicAACytoplasmicN/AyesyesNo340.193CYT0-0.200913-00NiCytoplasmic1070
DGOK_ECOLIP31459
dgoK

yidV

yidVb3693 JW3670 cytoplasmic protein, 2-dehydro-3-deoxygalactonokinase DgoKCytoplasmicAACytoplasmicIM (predicted)noyesNo340.174CYT0-0.200913-00NiUnknown27
KDGK_ECOLIP37647
kdgK

yhjI

yhjIb3526 JW5668 cytoplasmic protein, 2-dehydro-3-deoxygluconokinase KdgKCytoplasmicAACytoplasmicN/AyesyesNo540.131CYT0-0.200913-00NiUnknown2
KDSA_ECOLIP0A715
kdsA
b1215 JW1206 cytoplasmic protein, 2-dehydro-3-deoxyphosphooctonate aldolase KdsACytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo560.121CYT0-0.200913-00NiCytoplasmic1029
PANE_ECOLIP0A9J4
panE

apbA

apbAb0425 JW0415 cytoplasmic protein, 2-dehydropantoate 2-reductase PanECytoplasmic (By similarity) Cytoplasmic (Experimental)Phobious predicts the sequence that follows signal peptide as non cytoplasmic and non transmembrane. The native enzyme exists in solution as a monomer with a molecular mass of 34 000 Da. PMID: 10736170 Lipoprotein PMID: 10572132 PMID: 15174130 PMID: 10736170PMID: 10572132 PMID: 15174130CytoplasmicAACytoplasm Cytoplasmic (Experimental)IM (predicted)LOL: Localization Of LipoproteinsyesyesNo210.133SpII192.5798Pos+2=K00Yn5-16c20/21oCytoplasmic831
GARR_ECOLIP0ABQ2
garR

yhaE

yhaEb3125 JW5526 cytoplasmic protein, 2-hydroxy-3-oxopropionate reductase GarRCytoplasmicAACytoplasmicN/AyesyesNo380.214CYT0-0.200913-00NiCytoplasmic990
GLXR_ECOLIP77161
glxR

glxB1 ybbQ

glxB1 ybbQb0509 JW0497 cytoplasmic protein, 2-hydroxy-3-oxopropionate reductase GlxRCytoplasmicAACytoplasmicN/AyesyesNo230.151CYT0-0.200913-00Yn3-10c22/23oCytoplasmic971
MHPC_ECOLIP77044
mhpC
b0349 JW0340 cytoplasmic protein, 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase MhpCCytoplasmicAACytoplasmicN/AnoyesNo520.107CYT0-0.200913-00NiCytoplasmic816
THIH_ECOLIP30140
thiH
b3990 JW3953 cytoplasmic protein, 2-iminoacetate synthase ThiHCytoplasmicAACytoplasmicN/AyesyesNo260.141CYT0-0.200913-00NiUnknown2
LEU1_ECOLIP09151
leuA
b0074 JW0073 cytoplasmic protein, 2-isopropylmalate synthase LeuACytoplasmicAACytoplasmicN/AnoyesNo350.106CYT0-0.200913-00NiCytoplasmic99
RHMA_ECOLIP76469
rhmA

yfaU

yfaUb2245 JW2239 cytoplasmic protein, 2-keto-3-deoxy-L-rhamnonate aldolase RhmACytoplasmicAACytoplasmicIM (predicted)yesyesNo320.417CYT0-0.200913-00NiCytoplasmic96
MHPD_ECOLIP77608
mhpD
b0350 JW0341 cytoplasmic protein, 2-keto-4-pentenoate hydratase MhpDCytoplasmicAACytoplasmicN/AnoyesNo380.173CYT0-0.200913-00NiCytoplasmic874
PRPD_ECOLIP77243
prpD

yahT

yahTb0334 JW0325 cytoplasmic protein, 2-methylcitrate dehydratase PrpDCytoplasmicAACytoplasmicN/AnoyesNo340.119CYT0-0.200913-00NiCytoplasmic910
PRPC_ECOLIP31660
prpC

yahS yzzD

yahS yzzDb0333 JW0324 cytoplasmic protein, 2-methylcitrate synthase PrpCCytoplasmicAACytoplasmicN/AnoyesNo340.114CYT0-0.200913-00NiCytoplasmic992
UBIF_ECOLIP75728
ubiF

yleB

yleBb0662 JW0659 cytoplasmic protein, 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase UbiFNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)yesyesNo310.178TMH01.41397-00NiUnknown214
UBII_ECOLIP25535
ubiI

visC

visCb2906 JW2874 cytoplasmic protein, 2-octaprenylphenol hydroxylase UbiIUncharacterized conserved visC gene is involved in Q biosynthesis in Escherichia coli (renamed as ubiI). Coenzyme Q (ubiquinone or Q) is a widespread redox-active lipid essential for electron and proton transport in the respiratory chains from bacteria to mammals. VisC/UbiL protein functions in the C5-hydroxylation reaction.CytoplasmicAAMembrane LipoproteinIM (predicted-detected)LOL: Localization Of LipoproteinsyesyesNo200.198SpII190.428013Pos+2=G00NiCytoplasmic949
OTNI_ECOLIQ46891
otnI

ygbM

ygbMb2739 JW2709 cytoplasmic protein, 2-oxo-tetronate isomerase OtnICytoplasmicAACytoplasmicN/AnoyesNo690.144CYT0-0.200913-00NiCytoplasmic914
MEND_ECOLIP17109
menD
b2264 JW5374 cytoplasmic protein, 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase MenDCytoplasmicAACytoplasmicN/AyesyesNo410.136CYT0-0.200913-00NiCytoplasmicMembrane93
MENH_ECOLIP37355
menH

yfbB

yfbBb2263 JW2258 cytoplasmic protein, 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase MenHCytoplasmicAACytoplasmicN/AyesyesNo300.146CYT0-0.200913-00NiUnknown2
RLMA_ECOLIP36999
rlmA

rrmA yebH

rrmA yebHb1822 JW1811 cytoplasmic protein, 23S rRNA (guanine(745)-N(1))-methyltransferase RlmACytoplasmicAACytoplasmicN/AyesyesNo100.104CYT0-0.200913-00NiCytoplasmic1036
RLMC_ECOLIP75817
rlmC

rumB ybjF

rumB ybjFb0859 JW0843 cytoplasmic protein, 23S rRNA (uracil(747)-C(5))-methyltransferase RlmCTranslation, Ribosomal.CytoplasmicAACytoplasmicN/AyesyesNo280.107CYT0-0.200913-00NiCytoplasmic915
YDCP_ECOLIP76104
ydcP
b1435 JW1431 cytoplasmic protein, 23S rRNA 5-hydroxycytidine C2501 synthase YdcPCytoplasmicAACytoplasmicN/AnoyesNo180.121CYT0-0.200913-00NiCytoplasmic813
RLMB_ECOLIP63177
rlmB

yjfH

yjfHb4180 JW4138 cytoplasmic protein, 23S rRNA RlmBCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo240.115CYT0-0.200913-00NiCytoplasmic993
RLMD_ECOLIP55135
rlmD

rumA ygcA

rumA ygcAb2785 JW2756 cytoplasmic protein, 23S rRNA RlmDCytoplasmicAACytoplasmicN/AnoyesNo510.112CYT0-0.200913-00NiCytoplasmic9
FER_ECOLIP0A9R4
fdx
b2525 JW2509 cytoplasmic protein, 2Fe-2S ferredoxin FdxCytoplasmicAACytoplasmicN/AyesyesNo420.124CYT0-0.200913-00NiCytoplasmic882
CPDA_ECOLIP0AEW4
cpdA

icc

iccb3032 JW3000 cytoplasmic protein, 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdACytoplasmicAACytoplasmicN/AyesyesNo670.133CYT0-0.200913-00NiCytoplasmic960
TATD_ECOLIP27859
tatD

mttC yigW yigX

mttC yigW yigXb4483 JW5931 cytoplasmic protein, 3'-5' ssDNA/RNA exonuclease TatDCytoplasmic (Probable) Cytoplasmic (Experimental)PMID: 10747959 PMID: 9298646CytoplasmicAACytoplasm Cytoplasmic (Experimental)N/Apseudogene (Ochman & Davalos, 2006)noyesNo150.109CYT0-0.200913-00NiCytoplasmic10
MHPA_ECOLIP77397
mhpA
b0347 JW0338 cytoplasmic protein, 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase MhpACytoplasmicAACytoplasmicIM (predicted)noyesNo320.135CYT0-0.200913-00NiUnknown27
AROD_ECOLIP05194
aroD
b1693 JW1683 cytoplasmic protein, 3-dehydroquinate dehydratase AroDCytoplasmicAACytoplasmicN/AyesyesNo370.183CYT0-0.200913-00NiCytoplasmic897
AROB_ECOLIP07639
aroB
b3389 JW3352 cytoplasmic protein, 3-dehydroquinate synthase AroBCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo290.12CYT0-0.200913-00NiCytoplasmic1022
KDSB_ECOLIP04951
kdsB
b0918 JW0901 cytoplasmic protein, 3-deoxy-manno-octulosonate cytidylyltransferase KdsBCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo610.107CYT0-0.200913-00NiCytoplasmic10115
LSRF_ECOLIP76143
lsrF

yneB

yneBb1517 JW1510 cytoplasmic protein, 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase LsrFCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo560.103CYT0-0.200913-00NiUnknown233
PAAH_ECOLIP76083
paaH

ydbU

ydbUb1395 JW1390 cytoplasmic protein, 3-hydroxyadipyl-CoA dehydrogenase PaaHNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo410.145CYT0-0.200913-00NiCytoplasmic97
FABA_ECOLIP0A6Q3
fabA
b0954 JW0937 cytoplasmic protein, 3-hydroxydecanoyl-(acyl-carrier-protein) dehydratase FabATrigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo310.116CYT0-0.200913-00NiCytoplasmic1065
LEUC_ECOLIP0A6A6
leuC
b0072 JW0071 cytoplasmic protein, 3-isopropylmalate dehydratase large subunit LeuC Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AnoyesNo410.123CYT0-0.200913-00NiCytoplasmic970
LEUD_ECOLIP30126
leuD
b0071 JW0070 cytoplasmic protein, 3-isopropylmalate dehydratase small subunit LeuDCytoplasmicAACytoplasmicIM (predicted)noyesNo180.111CYT0-0.200913-00NiCytoplasmic992
LEU3_ECOLIP30125
leuB
b0073 JW5807 cytoplasmic protein, 3-isopropylmalate dehydrogenase LeuBCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo220.115CYT0-0.200913-00NiCytoplasmic1058
SGBH_ECOLIP37678
sgbH

yiaQ

yiaQb3581 JW3553 cytoplasmic protein, 3-keto-L-gulonate-6-phosphate decarboxylase SgbHCytoplasmicAACytoplasmicN/AyesyesNo180.147CYT0-0.200913-00NiCytoplasmic841
ULAD_ECOLIP39304
ulaD

sgaH yjfV

sgaH yjfVb4196 JW4154 cytoplasmic protein, 3-keto-L-gulonate-6-phosphate decarboxylase UlaDCytoplasmicAACytoplasmicN/AyesyesNo470.112CYT0-0.200913-00NiCytoplasmic8
FADA_ECOLIP21151
fadA

oldA

oldAb3845 JW5578 cytoplasmic protein, 3-ketoacyl-CoA thiolase FadACytoplasmicAACytoplasmCytoplasmicN/AyesyesNo280.133CYT0-0.200913-00NiCytoplasmic910
THTM_ECOLIP31142
sseA
b2521 JW2505 cytoplasmic protein, 3-mercaptopyruvate sulfurtransferase SseACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo100.108CYT0-0.200913-00NiUnknown5
PANB_ECOLIP31057
panB
b0134 JW0130 cytoplasmic protein, 3-methyl-2-oxobutanoate hydroxymethyltransferase PanBCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo350.122CYT0-0.200913-00NiCytoplasmic843
OTNC_ECOLIQ46890
otnC

ygbL

ygbLb2738 JW2708 cytoplasmic protein, 3-oxo-tetronate 4-phosphate decarboxylase OtnCCytoplasmicAACytoplasmicN/AnoyesNo470.21CYT0-0.200913-00NiUnknown256
OTNK_ECOLIQ46889
otnK

ygbK

ygbKb2737 JW2707 cytoplasmic protein, 3-oxo-tetronate kinase OtnKCytoplasmicAACytoplasmicN/AnoyesNo610.125CYT0-0.200913-00NiUnknown220
FABH_ECOLIP0A6R0
fabH
b1091 JW1077 cytoplasmic protein, 3-oxoacyl-(acyl-carrier-protein) synthase 3 FabHCytoplasmicAACytoplasm CytoplasmicIM (predicted)yesyesNo210.114CYT0-0.200913-00NiCytoplasmic1091
PAAJ_ECOLIP0C7L2
paaJ

paaE ydbW

paaE ydbWb1397 JW1392 cytoplasmic protein, 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase PaaJCytoplasmicAACytoplasmicN/AyesyesNo460.125CYT0-0.200913-00NiCytoplasmic918
HCAB_ECOLIP0CI31
hcaB

phdD yfhX

phdD yfhXb2541 JW2525 cytoplasmic protein, 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase HcaBCytoplasmicAACytoplasmicN/AnoyesNo340.119CYT0-0.200913-00NiCytoplasmic99
HCAC_ECOLIP0ABW0
hcaC

hcaA3 phdB yfhW

hcaA3 phdB yfhWb2540 JW2524 cytoplasmic protein, 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit HcaCCytoplasmicAACytoplasmicN/AnoyesNo380.146CYT0-0.200913-00NiUnknown2112
HCAD_ECOLIP77650
hcaD

hcaA4 phdA yfhY

hcaA4 phdA yfhYb2542 JW2526 cytoplasmic protein, 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component HcaDCytoplasmicAACytoplasmicN/AnoyesNo290.119CYT0-0.200913-00NiCytoplasmic940
HCAE_ECOLIP0ABR5
hcaE

digA hcaA hcaA1 phdC1 yfhU

digA hcaA hcaA1 phdC1 yfhUb2538 JW2522 cytoplasmic protein, 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha HcaECytoplasmicAACytoplasmicN/AnoyesNo480.107CYT0-0.200913-00NiCytoplasmic913
HCAF_ECOLIQ47140
hcaF

digB hcaA2 hcaB phdC2 yfhV

digB hcaA2 hcaB phdC2 yfhVb2539 JW2523 cytoplasmic protein, 3-phenylpropionate/cinnamic acid dioxygenase subunit beta HcaFCytoplasmicAACytoplasmicN/AnoyesNo510.125CYT0-0.200913-00NiCytoplasmic96
AROA_ECOLIP0A6D3
aroA
b0908 JW0891 cytoplasmic protein, 3-phosphoshikimate 1-carboxyvinyltransferase AroACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo380.15CYT0-0.200913-00NiCytoplasmic861
SQUU_ECOLIP0A9V8
yihU
b3882 JW3853 cytoplasmic protein, 3-sulfolactaldehyde reductase YihUCytoplasmicAACytoplasmicN/AyesyesNo180.352CYT0-0.200913-00Yn2-9c17/18oCytoplasmic914
ACPT_ECOLIP37623
acpT

yhhU

yhhUb3475 JW3440 cytoplasmic protein, 4'-phosphopantetheinyl transferase AcpTCytoplasmicAACytoplasmicN/AyesyesNo630.119CYT0-0.200913-00NiUnknown213
YGID_ECOLIP24197
ygiD
b3039 JW3007 cytoplasmic protein, 4,5-DOPA dioxygenase extradiol YgiDCytoplasmicAACytoplasm Integral Membrane ProteinN/AyesyesNo220.203CYT0-0.200913-00NiUnknown2
PUUE_ECOLIP50457
puuE

goaG

goaGb1302 JW1295 cytoplasmic protein, 4-aminobutyrate aminotransferase PuuECytoplasmicAACytoplasmicN/AyesyesNo290.166CYT0-0.200913-00NiCytoplasmic942
KDUI_ECOLIQ46938
kduI

yqeE

yqeEb2843 JW2811 cytoplasmic protein, 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase KduICytoplasmicAACytoplasmicN/AyesyesNo130.123CYT0-0.200913-00NiCytoplasmic863
ISPE_ECOLIP62615
ispE

ipk ychB

ipk ychBb1208 JW1199 cytoplasmic protein, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspECytoplasmicAACytoplasmicN/AyesyesNo230.205CYT0-0.200913-00NiCytoplasmic899
HOA_ECOLIP51020
mhpE
b0352 JW0343 cytoplasmic protein, 4-hydroxy-2-oxovalerate aldolase MhpECytoplasmicAACytoplasmicN/AnoyesNo500.105CYT0-0.200913-00NiCytoplasmic109
ISPH_ECOLIP62623
ispH

lytB yaaE

lytB yaaEb0029 JW0027 cytoplasmic protein, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase IspHCytoplasmicAACytoplasmicN/AyesyesNo280.217CYT0-0.200913-00NiCytoplasmic975
DAPB_ECOLIP04036
dapB
b0031 JW0029 cytoplasmic protein, 4-hydroxy-tetrahydrodipicolinate reductase DapBCytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo250.144CYT0-0.200913-00NiCytoplasmic1094
DAPA_ECOLIP0A6L2
dapA
b2478 JW2463 cytoplasmic protein, 4-hydroxy-tetrahydrodipicolinate synthase DapACytoplasmicAACytoplasmCytoplasmicN/AyesyesNo220.116CYT0-0.200913-00NiCytoplasmic10
PDXA_ECOLIP19624
pdxA
b0052 JW0051 cytoplasmic protein, 4-hydroxythreonine-4-phosphate dehydrogenase PdxACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo660.214CYT0-0.200913-00NiCytoplasmic994
RNAAM_ECOLIP77766
yciV

trpH

trpHb1266 JW1258 cytoplasmic protein, 5'-3' exoribonuclease YciVCytoplasmicAACytoplasmicN/AyesyesNo210.111CYT0-0.200913-00NiCytoplasmic958
5DNU_ECOLIP76491
yfbR
b2291 JW2288 cytoplasmic protein, 5'-deoxynucleotidase YfbRCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo460.185CYT0-0.200913-00NiCytoplasmic867
MTNN_ECOLIP0AF12
mtnN

mtn pfs yadA

mtn pfs yadAb0159 JW0155 cytoplasmic protein, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase MtnNCytoplasmicAACytoplasmicN/AyesyesNo560.13CYT0-0.200913-00NiCytoplasmic8106
SURE_ECOLIP0A840
surE

ygbC

ygbCb2744 JW2714 cytoplasmic protein, 5'/3'-nucleotidase SurECytoplasmicAACytoplasm CytoplasmicN/AyesyesNo340.117CYT0-0.200913-00NiUnknown240
METF_ECOLIP0AEZ1
metF
b3941 JW3913 cytoplasmic protein, 5,10-methylenetetrahydrofolate reductase MetFCytoplasmicAACytoplasmicN/AyesyesNo180.111CYT0-0.200913-00NiCytoplasmic925
YBJI_ECOLIP75809
ybjI
b0844 JW5113 cytoplasmic protein, 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YbjICytoplasmicAACytoplasmicN/AyesyesNo660.127CYT0-0.200913-00NiCytoplasmic859
YIGB_ECOLIP0ADP0
yigB
b3812 JW3785 cytoplasmic protein, 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigBCytoplasmicAACytoplasmicN/AProphage IntegraseyesnoNo260.103CYT0-0.200913-00NiCytoplasmic911
5FCL_ECOLIP0AC28
ygfA
b2912 JW2879 cytoplasmic protein, 5-formyltetrahydrofolate cyclo-ligase YgfACytoplasmicAACytoplasmicN/AyesyesNo350.242CYT0-0.200913-02Ni21-43o63-81iUnknown29
GARL_ECOLIP23522
garL

yhaF

yhaFb3126 JW3095 cytoplasmic protein, 5-keto-4-deoxy-D-glucarate aldolase GarLCytoplasmicAACytoplasmicN/AyesyesNo290.122CYT0-0.200913-00NiCytoplasmic9
IDNO_ECOLIP0A9P9
idnO

yjgU

yjgUb4266 JW4223 cytoplasmic protein, 5-keto-D-gluconate 5-reductase IdnOCytoplasmicAACytoplasmicN/AnoyesNo210.181CYT0-0.200913-00NiCytoplasmic948
MCRA_ECOLIP24200
mcrA

rglA

rglAb1159 JW1145 cytoplasmic protein, 5-methylcytosine-specific restriction enzyme A McrACytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo390.103CYT0-0.200913-00NiCytoplasmic839
METE_ECOLIP25665
metE
b3829 JW3805 cytoplasmic protein, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetECytoplasmicAACytoplasmicN/AyesyesNo230.107CYT0-0.200913-00NiCytoplasmic833
YBGL_ECOLIP75746
ybgL
b0713 JW0703 cytoplasmic protein, 5-oxoprolinase subunit A YbgLCytoplasmicAACytoplasmicN/AyesyesNo300.197CYT0-0.200913-00NiCytoplasmic811
YBGJ_ECOLIP0AAV4
ybgJ
b0711 JW0701 cytoplasmic protein, 5-oxoprolinase subunit B YbgJCytoplasmicAACytoplasmicN/AyesyesNo260.194CYT0-0.200913-00NiCytoplasmic918
YBGK_ECOLIP75745
ybgK
b0712 JW0702 cytoplasmic protein, 5-oxoprolinase subunit C YbgKCytoplasmicAACytoplasmicN/AyesyesNo510.129CYT0-0.200913-00NiCytoplasmic96
ROXA_ECOLIP27431
roxA

ycfD

ycfDb1128 JW1114 cytoplasmic protein, 50S L16 3-hydroxylase RoxACytoplasmicAACytoplasmicN/AyesyesNo420.111CYT0-0.200913-00NiCytoplasmic981
PRMB_ECOLIP39199
prmB

yfcB

yfcBb2330 JW5841 cytoplasmic protein, 50S L3 glutamine methyltransferase PrmBCytoplasmicAACytoplasmicN/AyesyesNo380.133CYT0-0.200913-00NiCytoplasmic932
QUED_ECOLIP65870
queD

ygcM

ygcMb2765 JW2735 cytoplasmic protein, 6-carboxy-5,6,7,8-tetrahydropterin synthase QueDCytoplasmicAACytoplasmicN/AyesyesNo180.109CYT0-0.200913-00NiCytoplasmic990
ASCB_ECOLIP24240
ascB
b2716 JW2686 cytoplasmic protein, 6-phospho-beta-glucosidase AscBCytoplasmicAACytoplasmicN/AyesyesNo160.219CYT0-0.200913-00NiCytoplasmic928
BGLA_ECOLIQ46829
bglA

bglD yqfC

bglD yqfCb2901 JW2869 cytoplasmic protein, 6-phospho-beta-glucosidase BglATrigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo200.217CYT0-0.200913-00NiCytoplasmic969
BGLB_ECOLIP11988
bglB
b3721 JW3699 cytoplasmic protein, 6-phospho-beta-glucosidase BglBCytoplasmicAACytoplasmicN/AyesyesNo160.139CYT0-0.200913-00NiCytoplasmic919
CHBF_ECOLIP17411
chbF

celF ydjD

celF ydjDb1734 JW1723 cytoplasmic protein, 6-phospho-beta-glucosidase ChbFCytoplasmicAACytoplasmicN/AyesyesNo630.113CYT0-0.200913-00NiCytoplasmic824
YIEH_ECOLIP31467
yieH
b3715 JW3693 cytoplasmic protein, 6-phosphogluconate phosphatase YieHCytoplasmicAACytoplasmicN/AnoyesNo680.111CYT0-0.200913-00NiCytoplasmic923
6PGL_ECOLIP52697
pgl

ybhE

ybhEb0767 JW0750 cytoplasmic protein, 6-phosphogluconolactonase PglCytoplasmicAACytoplasmicN/AnoyesNo410.114CYT0-0.200913-00NiPeriplasmic984
HDHA_ECOLIP0AET8
hdhA

hsdH

hsdHb1619 JW1611 cytoplasmic protein, 7-alpha-hydroxysteroid dehydrogenase HdhA Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AyesyesNo480.2CYT0-0.200913-00NiCytoplasmic968
QUEE_ECOLIP64554
queE

ygcF

ygcFb2777 JW2748 cytoplasmic protein, 7-carboxy-7-deazaguanine synthase QueEOld gene name YgaW.CytoplasmicAACytoplasmicN/AyesyesNo390.13CYT0-0.200913-00NiCytoplasmic963
QUEC_ECOLIP77756
queC

ybaX

ybaXb0444 JW0434 cytoplasmic protein, 7-cyano-7-deazaguanine synthase QueCCytoplasmicAACytoplasmicIM (predicted)yesyesNo220.126CYT0-0.200913-00NiCytoplasmic8104
BIOF_ECOLIP12998
bioF
b0776 JW0759 cytoplasmic protein, 8-amino-7-oxononanoate synthase BioFCytoplasmicAACytoplasmicN/AyesyesNo300.112CYT0-0.200913-00NiCytoplasmic93
MUTT_ECOLIP08337
mutT
b0099 JW0097 cytoplasmic protein, 8-oxo-dGTP diphosphatase MutTCytoplasmicAACytoplasmicN/AyesyesNo250.11CYT0-0.200913-00NiCytoplasmic8107
ACDH_ECOLIP77580
mhpF

mhpE

mhpEb0351 JW0342 cytoplasmic protein, Acetaldehyde dehydrogenase MhpFCytoplasmicAACytoplasmicN/AnoyesNo700.129CYT0-0.200913-00NiCytoplasmic858
ATOD_ECOLIP76458
atoD
b2221 JW2215 cytoplasmic protein, Acetate CoA-transferase subunit alpha AtoDCytoplasmicAACytoplasmicN/AnoyesNo390.125TMH00.259434-00NiCytoplasmic954
ATOA_ECOLIP76459
atoA
b2222 JW2216 cytoplasmic protein, Acetate CoA-transferase subunit beta AtoACytoplasmicAACytoplasmicN/AnoyesNo610.12CYT0-0.200913-00NiCytoplasmic925
YDIF_ECOLIP37766
ydiF
b1694 JW1684 cytoplasmic protein, Acetate CoA-transferase YdiF YdiFCytoplasmicAACytoplasmicN/AnoyesNo520.132CYT0-0.200913-00NiCytoplasmic833
ILVB_ECOLIP08142
ilvB
b3671 JW3646 cytoplasmic protein, Acetolactate synthase isozyme 1 large subunit IlvB Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmicN/AyesyesNo450.118CYT0-0.200913-00NiCytoplasmic929
ILVN_ECOLIP0ADF8
ilvN
b3670 JW3645 cytoplasmic protein, Acetolactate synthase isozyme 1 small subunit IlvNCytoplasmicAACytoplasmicN/AnoyesNo360.123CYT0-0.200913-00NiCytoplasmic999
ILVM_ECOLIP0ADG1
ilvM
b3769 JW3742 cytoplasmic protein, Acetolactate synthase isozyme 2 small subunit IlvMCytoplasmicAACytoplasmicN/AnoyesNo430.131CYT0-0.200913-00NiUnknown257
ILVI_ECOLIP00893
ilvI
b0077 JW0076 cytoplasmic protein, Acetolactate synthase isozyme 3 large subunit IlvICytoplasmicAACytoplasmicN/AyesyesNo250.127CYT0-0.200913-00NiCytoplasmic93
ILVH_ECOLIP00894
ilvH

brnP

brnPb0078 JW0077 cytoplasmic protein, Acetolactate synthase isozyme 3 small subunit IlvHCytoplasmicAACytoplasmicN/AnoyesNo180.108CYT0-0.200913-00Yn4-9c17/18oCytoplasmic915
AES_ECOLIP23872
aes

ybaC

ybaCb0476 JW0465 cytoplasmic protein, Acetyl esterase AesCytoplasmic (Probable)PMID: 9576853CytoplasmicAACytoplasm CytoplasmicN/AyesyesNo340.108CYT0-0.200913-00NiCytoplasmic1034
ATOB_ECOLIP76461
atoB
b2224 JW2218 cytoplasmic protein, Acetyl-CoA acetyltransferase AtoBCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo230.375CYT0-0.200913-00NiCytoplasmic98
ACOCT_ECOLIP76518
yfdE
b2371 JW2368 cytoplasmic protein, Acetyl-CoA:oxalate CoA-transferase YfdENo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)yesyesNo480.144CYT0-0.200913-00NiCytoplasmic936
ARGD_ECOLIP18335
argD

dapC dtu

dapC dtub3359 JW3322 cytoplasmic protein, Acetylornithine/succinyldiaminopimelate aminotransferase ArgDCopurifies with trigger factor in pull down assays.CytoplasmicAACytoplasm CytoplasmicN/ATFyesyesNo250.129CYT0-0.200913-00NiCytoplasmic929
YDCK_ECOLIP76100
ydcK
b1428 JW1424 cytoplasmic protein, Acetyltransferase YdcKCytoplasmicAACytoplasmicN/AnoyesNo550.163CYT0-0.200913-00NiCytoplasmic818
YPEA_ECOLIP76539
ypeA
b2434 JW2427 cytoplasmic protein, Acetyltransferase YpeACytoplasmicAACytoplasmicN/AyesyesNo530.103CYT0-0.200913-00NiCytoplasmic880
MSYB_ECOLIP25738
msyB
b1051 JW1039 cytoplasmic protein, Acidic MsyBCytoplasmicAACytoplasmicN/AyesyesNo560.112CYT0-0.200913-00NiCytoplasmic891
ACNA_ECOLIP25516
acnA

acn

acnb1276 JW1268 cytoplasmic protein, Aconitate hydratase A AcnACytoplasmic (Experimental)PMID: 10585860CytoplasmicAACytoplasmic (Experimental)N/AnoyesNo470.106CYT0-0.200913-00NiCytoplasmic940
ACPH_ECOLIP21515
acpH

yajB

yajBb0404 JW0394 cytoplasmic protein, Acyl carrier phosphodiesterase AcpHCytoplasmicAACytoplasmicN/AnoyesNo240.265CYT0-0.200913-00Yn3-13c23/24oCytoplasmic813
LPXA_ECOLIP0A722
lpxA
b0181 JW0176 cytoplasmic protein, Acyl-(acyl-carrier-protein)--UDP-N-acetylglucosamine O-acyltransferase LpxACytoplasmicAACytoplasmCytoplasmicN/AyesyesNo250.206CYT0-0.200913-00NiCytoplasmic1021
YCIA_ECOLIP0A8Z0
yciA
b1253 JW1245 cytoplasmic protein, Acyl-CoA thioester hydrolase YciACytoplasmicAACytoplasmicN/AyesyesNo260.145CYT0-0.200913-00NiUnknown279
TESB_ECOLIP0AGG2
tesB
b0452 JW0442 cytoplasmic protein, Acyl-CoA thioesterase 2 TesBCytoplasmicAACytoplasmicN/AyesyesNo470.117CYT0-0.200913-00NiCytoplasmic923
PAAI_ECOLIP76084
paaI

ydbV

ydbVb1396 JW1391 cytoplasmic protein, Acyl-coenzyme A thioesterase PaaICytoplasmicAACytoplasmicN/AnoyesNo680.192CYT0-0.200913-00NiUnknown276
ACYP_ECOLIP0AB65
yccX
b0968 JW5131 cytoplasmic protein, Acylphosphatase YccX Disulfide bridge (5 49) PMID: 17134700CytoplasmicAACytoplasmicN/AnoyesNo280.119CYT0-0.200913-00Yn4-11c16/17oCytoplasmic9103
ADEC_ECOLIP31441
ade

yicP

yicPb3665 JW3640 cytoplasmic protein, Adenine deaminase AdeCytoplasmicAACytoplasmicIM (predicted)noyesNo310.144CYT0-0.200913-00NiCytoplasmic834
MUTY_ECOLIP17802
mutY

micA

micAb2961 JW2928 cytoplasmic protein, Adenine DNA glycosylase MutYCytoplasmicAACytoplasmicN/AyesyesNo580.111CYT0-0.200913-00NiCytoplasmic931
APT_ECOLIP69503
apt
b0469 JW0458 cytoplasmic protein, Adenine phosphoribosyltransferase AptCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo560.174CYT0-0.200913-00NiCytoplasmic989
COBC_ECOLIP52086
cobC

phpB

phpBb0638 JW0633 cytoplasmic protein, Adenosylcobalamin/alpha-ribazole phosphatase CobCCytoplasmicAACytoplasmicN/AyesyesNo360.16CYT0-0.200913-00NiCytoplasmic935
BIOA_ECOLIP12995
bioA
b0774 JW0757 cytoplasmic protein, Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo550.168CYT0-0.200913-00NiCytoplasmic1021
CYAA_ECOLIP00936
cyaA

cya

cyab3806 JW3778 cytoplasmic protein, Adenylate cyclase CyaACytoplasmicAACytoplasmCytoplasmicN/AnoyesNo260.196CYT0-0.200913-00NiCytoplasmic9
CYSC_ECOLIP0A6J1
cysC
b2750 JW2720 cytoplasmic protein, Adenylyl-sulfate kinase CysCCytoplasmicAACytoplasmicIM (predicted)yesyesNo480.15CYT0-0.200913-00NiCytoplasmic983
NUDE_ECOLIP45799
nudE

yrfE

yrfEb3397 JW3360 cytoplasmic protein, ADP compounds hydrolase NudECytoplasmicAACytoplasmicN/AyesyesNo700.106CYT0-0.200913-00NiCytoplasmic962
AEGA_ECOLIP37127
aegA

yffG

yffGb2468 JW2452 cytoplasmic protein, AegACytoplasmicAACytoplasmicN/AyesyesNo180.136CYT0-0.200913-00NiCytoplasmic921
SPEB_ECOLIP60651
speB
b2937 JW2904 cytoplasmic protein, Agmatinase SpeBCytoplasmicAACytoplasmicN/AyesyesNo530.127CYT0-0.200913-00NiCytoplasmic967
ALR1_ECOLIP0A6B4
alr
b4053 JW4013 cytoplasmic protein, Alanine racemase, biosynthetic AlrCytoplasmicAACytoplasmicN/AyesyesNo380.208CYT0-0.200913-00NiCytoplasmic946
ALDB_ECOLIP37685
aldB

yiaX

yiaXb3588 JW3561 cytoplasmic protein, Aldehyde dehydrogenase B AldBCytoplasmicAACytoplasmicN/AnoyesNo380.103CYT0-0.200913-00NiCytoplasmic9
AHR_ECOLIP27250
ahr

yjgB

yjgBb4269 JW5761 cytoplasmic protein, Aldehyde reductase AhrNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo210.108CYT0-0.200913-00NiCytoplasmic952
GALM_ECOLIP0A9C3
galM
b0756 JW0739 cytoplasmic protein, Aldose 1-epimerase GalMCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo100.161CYT0-0.200913-00NiPeriplasmic10104
SSUD_ECOLIP80645
ssuD

ycbN

ycbNb0935 JW0918 cytoplasmic protein, Alkanesulfonate monooxygenase SsuDCytoplasmicAACytoplasmicN/AyesyesNo390.14CYT0-0.200913-00NiCytoplasmic9
ALLC_ECOLIP77425
allC

glxB7 ylbB

glxB7 ylbBb0516 JW0504 cytoplasmic protein, Allantoate amidohydrolase AllCCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo240.136CYT0-0.200913-00NiCytoplasmic825
ALLB_ECOLIP77671
allB

glxB3 ybbX

glxB3 ybbXb0512 JW0500 cytoplasmic protein, Allantoinase AllBCytoplasmicAACytoplasmicN/AnoyesNo320.118CYT0-0.200913-00NiCytoplasmic9106
ALLE_ECOLIP75713
allE

glxB6 ylbA

glxB6 ylbAb0515 JW0503 cytoplasmic protein, AllECytoplasmicAACytoplasm CytoplasmicN/AyesyesNo280.109CYT0-0.200913-00NiCytoplasmic9
YIHX_ECOLIP0A8Y3
yihX
b3885 JW5566 cytoplasmic protein, Alpha-D-glucose 1-phosphate phosphatase YihXCytoplasmicAACytoplasmicN/AyesyesNo220.142CYT0-0.200913-00NiCytoplasmic8
PHNJ_ECOLIP16688
phnJ
b4098 JW4059 cytoplasmic protein, Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase PhnJCytoplasmicAACytoplasmicN/AnoyesNo110.114CYT0-0.200913-00NiCytoplasmic88
PHNM_ECOLIP16689
phnM
b4095 JW4056 cytoplasmic protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase PhnMCytoplasmicAACytoplasmicN/AyesyesNo310.131CYT0-0.200913-00NiCytoplasmic99
PHNG_ECOLIP16685
phnG
b4101 JW4062 cytoplasmic protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnGCytoplasmicAACytoplasmicN/AyesyesNo250.134CYT0-0.200913-00NiUnknown226
PHNH_ECOLIP16686
phnH
b4100 JW4061 cytoplasmic protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnHCytoplasmicAACytoplasmicN/AyesyesNo650.112CYT0-0.200913-00NiUnknown248
PHNI_ECOLIP16687
phnI
b4099 JW4060 cytoplasmic protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnICytoplasmicAACytoplasmicN/AyesyesNo180.115CYT0-0.200913-00NiCytoplasmic810
AGAL_ECOLIP06720
melA

mel-7

mel-7b4119 JW4080 cytoplasmic protein, Alpha-galactosidase MelACytoplasmicAACytoplasmicN/AyesyesNo240.108CYT0-0.200913-00Yn7-18c23/24oCytoplasmic820
TAUD_ECOLIP37610
tauD

ssiD yaiG

ssiD yaiGb0368 JW0360 cytoplasmic protein, Alpha-ketoglutarate-dependent taurine dioxygenase TauDCytoplasmicAACytoplasmicN/AyesyesNo220.149CYT0-0.200913-00NiCytoplasmic938
XYLS_ECOLIP31434
yicI
b3656 JW3631 cytoplasmic protein, Alpha-xylosidase YicICytoplasmicAACytoplasmicN/AnoyesNo250.174CYT0-0.200913-00NiUnknown231
ARFA_ECOLIP36675
arfA

yhdL

yhdLb4550 JW3253 cytoplasmic protein, Alternative ribosome-rescue factor A ArfACytoplasmicAACytoplasmicN/AnoyesNo290.112CYT0-0.200913-00NiUnknown2135
UXAB_ECOLIP0A6L7
uxaB
b1521 JW1514 cytoplasmic protein, Altronate oxidoreductase UxaBCytoplasmicAACytoplasmicN/AnoyesNo640.106CYT0-0.200913-00NiCytoplasmic980
ARGA_ECOLIP0A6C5
argA
b2818 JW2786 cytoplasmic protein, Amino-acid acetyltransferase ArgACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo600.101CYT0-0.200913-00NiCytoplasmic957
PHNO_ECOLIP16691
phnO
b4093 JW4054 cytoplasmic protein, Aminoalkylphosphonate N-acetyltransferase PhnOCytoplasmicAACytoplasmicN/AnoyesNo280.117CYT0-0.200913-00NiUnknown266
PABC_ECOLIP28305
pabC
b1096 JW1082 cytoplasmic protein, Aminodeoxychorismate lyase PabCCytoplasmicAACytoplasmicN/AyesyesNo300.109CYT0-0.200913-00NiCytoplasmic911
PABB_ECOLIP05041
pabB
b1812 JW1801 cytoplasmic protein, Aminodeoxychorismate synthase component 1 PabBCytoplasmicAACytoplasmicN/AnoyesNo420.126CYT0-0.200913-00Yn4-12c17/18oCytoplasmic982
PABA_ECOLIP00903
pabA
b3360 JW3323 cytoplasmic protein, Aminodeoxychorismate synthase component 2 PabACytoplasmicAACytoplasmicN/AyesyesNo250.113CYT0-0.200913-00NiCytoplasmic9
YPDE_ECOLIP77585
ypdE
b2384 JW2381 cytoplasmic protein, Aminopeptidase YpdECytoplasmicAACytoplasmicN/AyesyesNo240.106CYT0-0.200913-00NiCytoplasmic992
YPDF_ECOLIP76524
ypdF
b2385 JW2382 cytoplasmic protein, Aminopeptidase YpdFCytoplasmicAACytoplasmicN/AyesyesNo190.122CYT0-0.200913-00NiCytoplasmic932
AMN_ECOLIP0AE12
amn
b1982 JW1963 cytoplasmic protein, AMP nucleosidase AmnCytoplasmicAACytoplasmicN/AyesyesNo280.119CYT0-0.200913-00NiCytoplasmic937
NORV_ECOLIQ46877
norV

flrD ygaI ygaJ ygaK

flrD ygaI ygaJ ygaKb2710 JW2680 cytoplasmic protein, Anaerobic nitric oxide reductase flavorubredoxin NorVCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo180.103CYT0-0.200913-00NiCytoplasmic1039
NRDD_ECOLIP28903
nrdD
b4238 JW4197 cytoplasmic protein, Anaerobic ribonucleoside-triphosphate reductase NrdDCytoplasmicAACytoplasmicN/AyesyesNo590.103CYT0-0.200913-00NiCytoplasmic959
NRDG_ECOLIP0A9N8
nrdG

yjgE

yjgEb4237 JW4196 cytoplasmic protein, Anaerobic ribonucleoside-triphosphate reductase-activating NrdGCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo300.17CYT0-0.200913-00NiCytoplasmic1068
ASLB_ECOLIP25550
aslB

atsB

atsBb3800 JW5594 cytoplasmic protein, Anaerobic sulfatase-maturating enzyme homolog AslBCytoplasmicAACytoplasmicN/AyesyesNo220.108CYT0-0.200913-00NiCytoplasmic915
YDEM_ECOLIP76134
ydeM
b1497 JW1492 cytoplasmic protein, Anaerobic sulfatase-maturating enzyme homolog YdeMCytoplasmicAACytoplasmicN/AyesyesNo320.118CYT0-0.200913-00NiCytoplasmic97
ANMK_ECOLIP77570
anmK

ydhH

ydhHb1640 JW1632 cytoplasmic protein, Anhydro-N-acetylmuramic acid kinase AnmKCytoplasmicAACytoplasmicN/AyesyesNo240.131CYT0-0.200913-00NiCytoplasmicMembrane955
ARPA_ECOLIP23325
arpA

arp yjaC

arp yjaCb4017 JW3977 cytoplasmic protein, Ankyrin repeat A ArpACytoplasmicAACytoplasmicN/AnoyesNo210.126CYT0-0.200913-00NiUnknown2
IRAD_ECOLIP39375
iraD

yjiD

yjiDb4326 JW5782 cytoplasmic protein, Anti-adapter IraDCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo390.204CYT0-0.200913-00NiUnknown270
IRAM_ECOLIP75987
iraM

elb1 elbA ycgW

elb1 elbA ycgWb1160 JW1147 cytoplasmic protein, Anti-adapter IraMCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo230.126CYT0-0.200913-00NiUnknown254
IRAP_ECOLIP0AAN9
iraP

yaiB

yaiBb0382 JW0373 cytoplasmic protein, Anti-adapter IraPCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo420.126CYT0-0.200913-00NiUnknown283
YAFW_ECOLIQ47684
yafW
b0246 JW0235 cytoplasmic protein, Antitoxin YafWCytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo240.107CYT0-0.200913-00NiUnknown214
YFJZ_ECOLIP52141
yfjZ
b2645 JW2626 cytoplasmic protein, Antitoxin YfjZCytoplasmicAACytoplasmicN/AProphage Integrase (EcoGene)nonoNo180.106CYT0-0.200913-00NiUnknown264
APAG_ECOLIP62672
apaG
b0050 JW0049 cytoplasmic protein, ApaGCytoplasmicAACytoplasmicN/AyesyesNo310.135CYT0-0.200913-00NiUnknown267
CITX_ECOLIP0A6G5
citX

ybdU

ybdUb0614 JW0606 cytoplasmic protein, Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase CitXCytoplasmicAACytoplasmicN/AyesyesNo480.133CYT0-0.200913-00NiCytoplasmic8
GUTQ_ECOLIP17115
gutQ

srlQ

srlQb2708 JW5431 cytoplasmic protein, Arabinose 5-phosphate isomerase GutQCytoplasmicAACytoplasmicN/AyesyesNo350.114CYT0-0.200913-00NiUnknown251
KDSD_ECOLIP45395
kdsD

yrbH

yrbHb3197 JW3164 cytoplasmic protein, Arabinose 5-phosphate isomerase KdsDCytoplasmicAACytoplasmicIM (predicted)yesyesNo690.13CYT0-0.200913-00NiCytoplasmic862
ASTA_ECOLIP0AE37
astA

ydjV

ydjVb1747 JW1736 cytoplasmic protein, Arginine N-succinyltransferase AstACytoplasmicAACytoplasmicN/AyesyesNo340.16CYT0-0.200913-00NiCytoplasmic912
SYR_ECOLIP11875
argS
b1876 JW1865 cytoplasmic protein, Arginine--tRNA ligase ArgSCytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo230.136CYT0-0.200913-00NiCytoplasmic924
AROM_ECOLIP0AE28
aroM
b0390 JW0381 cytoplasmic protein, AroMPhobius predicts signal peptide (1-20).CytoplasmicAACytoplasmicIM (predicted)yesyesNo240.115CYT0-0.200913-00Yn3-13c20/21oCytoplasmic8108
TYRB_ECOLIP04693
tyrB
b4054 JW4014 cytoplasmic protein, Aromatic-amino-acid aminotransferase TyrB Disulfide bond-containing protein PMID: 25664544CytoplasmicAACytoplasmCytoplasmicN/AyesyesNo100.122CYT0-0.200913-00NiCytoplasmic1038
ARSC_ECOLIP0AB96
arsC

arsG

arsGb3503 JW3470 cytoplasmic protein, Arsenate reductase ArsCCytoplasmicAACytoplasmicN/AyesyesNo140.101CYT0-0.200913-00NiCytoplasmic992
PAND_ECOLIP0A790
panD
b0131 JW0127 cytoplasmic protein, Aspartate 1-decarboxylase PanDCytoplasmic (Probable)PMID: 6767707CytoplasmicAACytoplasm CytoplasmicN/AyesyesNo620.203CYT0-0.200913-00NiUnknown275
PYRB_ECOLIP0A786
pyrB
b4245 JW4204 cytoplasmic protein, Aspartate carbamoyltransferase catalytic subunit PyrBTrigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo340.111CYT0-0.200913-00NiCytoplasmic978
PYRI_ECOLIP0A7F3
pyrI
b4244 JW4203 cytoplasmic protein, Aspartate carbamoyltransferase regulatory chain PyrICytoplasmic (Experimental)PMID: 3912513 PMID: 8740179CytoplasmicAACytoplasmic (Experimental)N/AyesyesNo370.104CYT0-0.200913-00NiUnknown2116
ASNA_ECOLIP00963
asnA
b3744 JW3722 cytoplasmic protein, Aspartate--ammonia ligase AsnACytoplasmicAACytoplasmCytoplasmicN/AyesyesNo450.101CYT0-0.200913-00NiCytoplasmic1090
YGHR_ECOLIP64572
yghR
b2984 JW2952 cytoplasmic protein, ATP-binding YghRNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo500.116CYT0-0.200913-00NiUnknown231
PFKB_ECOLIP06999
pfkB
b1723 JW5280 cytoplasmic protein, ATP-dependent 6-phosphofructokinase isozyme 2 PfkBCytoplasmicAACytoplasmicN/AyesyesNo580.118CYT0-0.200913-00NiCytoplasmic983
CLPS_ECOLIP0A8Q6
clpS

yljA

yljAb0881 JW0865 cytoplasmic protein, ATP-dependent Clp protease adapter ClpSCytoplasmicAACytoplasmicN/AyesyesNo530.102CYT0-0.200913-00NiCytoplasmic1062
CLPA_ECOLIP0ABH9
clpA

lopD

lopDb0882 JW0866 cytoplasmic protein, ATP-dependent Clp protease ATP-binding subunit ClpACytoplasmicAACytoplasmicN/AnoyesNo180.153CYT0-0.200913-00NiCytoplasmic1060
CLPX_ECOLIP0A6H1
clpX

lopC

lopCb0438 JW0428 cytoplasmic protein, ATP-dependent Clp protease ATP-binding subunit ClpXCytoplasmicAACytoplasmicN/AyesyesNo310.124CYT0-0.200913-00NiCytoplasmic969
CLPP_ECOLIP0A6G7
clpP

lopP

lopPb0437 JW0427 cytoplasmic protein, ATP-dependent Clp protease proteolytic subunit ClpPCytoplasmicAACytoplasmCytoplasmicIM (predicted)noyesNo310.108CYT0-0.200913-00NiCytoplasmic10
BIOD1_ECOLIP13000
bioD1
b0778 JW0761 cytoplasmic protein, ATP-dependent dethiobiotin synthetase 1 BioDCytoplasmicAACytoplasmCytoplasmicIM (predicted)yesyesNo360.141CYT0-0.200913-00NiCytoplasmic1027
BIOD2_ECOLIP0A6E9
bioD2

ynfK

ynfKb1593 JW5264 cytoplasmic protein, ATP-dependent dethiobiotin synthetase 2 BioDCytoplasmicAACytoplasm CytoplasmicIM (predicted)yesyesNo320.161CYT0-0.200913-00NiCytoplasmic989
DING_ECOLIP27296
dinG

rarB

rarBb0799 JW0784 cytoplasmic protein, ATP-dependent DNA helicase DinGCytoplasmicAACytoplasmicN/AyesyesNo360.104CYT0-0.200913-00NiCytoplasmic95
RECQ_ECOLIP15043
recQ
b3822 JW5855 cytoplasmic protein, ATP-dependent DNA helicase RecQCytoplasmicAACytoplasmicN/AnoyesNo50.114CYT0-0.200913-00NiCytoplasmic947
HSLV_ECOLIP0A7B8
hslV

htpO yiiC

htpO yiiCb3932 JW3903 cytoplasmic protein, ATP-dependent protease subunit HslVCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo300.118CYT0-0.200913-00NiCytoplasmic10
HRPB_ECOLIP37024
hrpB

yadO

yadOb0148 JW0144 cytoplasmic protein, ATP-dependent RNA helicase HrpBCytoplasmicAACytoplasmicN/AyesyesNo320.17CYT0-0.200913-00Yn6-17c26/27oCytoplasmicMembrane93
RAVA_ECOLIP31473
ravA

yieN

yieNb3746 JW3725 cytoplasmic protein, ATPase RavACytoplasmicAACytoplasmCytoplasmicIM (predicted)noyesNo690.182CYT0-0.200913-00NiCytoplasmic1027
LSRK_ECOLIP77432
lsrK

ydeV

ydeVb1511 JW1504 cytoplasmic protein, Autoinducer-2 kinase LsrKCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo270.149CYT0-0.200913-00NiCytoplasmic96
FTNA_ECOLIP0A998
ftnA

ftn gen-165 rsgA

ftn gen-165 rsgAb1905 JW1893 cytoplasmic protein, Bacterial non-heme ferritin FtnACopurifies with trigger factor in pull down assays.CytoplasmicAACytoplasmCytoplasmicN/ATFyesyesNo480.159CYT0-0.200913-00NiCytoplasmic1074
BFR_ECOLIP0ABD3
bfr
b3336 JW3298 cytoplasmic protein, Bacterioferritin BfrCytoplasmicAACytoplasmicN/AnoyesNo300.153CYT0-0.200913-00NiCytoplasmic1095
BFD_ECOLIP0AE56
bfd

yheA

yheAb3337 JW3299 cytoplasmic protein, Bacterioferritin-associated ferredoxin BfdCytoplasmicAACytoplasmicN/AnoyesNo180.109CYT0-0.200913-00NiCytoplasmic10
BAX_ECOLIP27297
bax
b3570 JW5653 cytoplasmic protein, BaxPotential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. ATP-binding,CytoplasmicAAPeriplasmicN/ASurAyesyesYes280.533SpI2713.0141-00Yn10-21c27/28oUnknown260
BDM_ECOLIP76127
bdm

yddX

yddXb1481 JW5239 cytoplasmic protein, BdmCytoplasmicAACytoplasmicN/AyesyesNo270.118CYT0-0.200913-00NiUnknown2
BEEE_ECOLIP75980
beeE

ymfO

ymfOb1151 JW1137 cytoplasmic protein, BeeECytoplasmicAACytoplasmicN/Apseudogene (Uniprot)nonoNo570.129CYT0-0.200913-00NiUnknown29
BGAL_ECOLIP00722
lacZ
b0344 JW0335 cytoplasmic protein, Beta-galactosidase LacZCytoplasmicAACytoplasmicN/AyesyesNo360.136CYT0-0.200913-00NiUnknown241
NAGZ_ECOLIP75949
nagZ

ycfO

ycfOb1107 JW1093 cytoplasmic protein, Beta-hexosaminidase NagZCytoplasmicAACytoplasmCytoplasmicN/AyesyesNo400.114CYT0-0.200913-00NiCytoplasmic1011
PGMB_ECOLIP77366
ycjU
b1317 JW1310 cytoplasmic protein, Beta-phosphoglucomutase YcjUCytoplasmicAACytoplasm CytoplasmicN/AnoyesNo300.157CYT0-0.200913-00NiUnknown2
COBU_ECOLIP0AE76
cobU
b1993 JW1971 cytoplasmic protein, Bifunctional adenosylcobalamin biosynthesis CobUCytoplasmicAACytoplasmicN/AnoyesNo200.144CYT0-0.200913-00NiCytoplasmic962
PHEA_ECOLIP0A9J8
pheA
b2599 JW2580 cytoplasmic protein, Bifunctional chorismate mutase/prephenate dehydratase PheACytoplasmicAACytoplasm CytoplasmicN/AyesyesNo330.122CYT0-0.200913-00NiCytoplasmic1044
FOLD_ECOLIP24186
folD

ads

adsb0529 JW0518 cytoplasmic protein, Bifunctional FolDCytoplasmicAACytoplasmicN/AyesyesNo490.145CYT0-0.200913-00NiCytoplasmic944
GLNE_ECOLIP30870
glnE
b3053 JW3025 cytoplasmic protein, Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme GlnECytoplasmicAACytoplasmicN/AyesyesNo330.21CYT0-0.200913-00NiCytoplasmicMembrane957
GSP_ECOLIP0AES0
gss

gsp

gspb2988 JW2956 cytoplasmic protein, Bifunctional glutathionylspermidine synthetase/amidase GssCytoplasmicAAMembrane anchoredN/AyesyesNo250.115CYT0-0.200913-10NiCytoplasmic850
HLDE_ECOLIP76658
hldE

rfaE waaE yqiF

rfaE waaE yqiFb3052 JW3024 cytoplasmic protein, Bifunctional HldECytoplasmicAACytoplasmicN/AyesyesNo680.175CYT0-0.200913-00NiCytoplasmic977
NNR_ECOLIP31806
nnr

yjeF

yjeFb4167 JW4125 cytoplasmic protein, Bifunctional NnrCytoplasmicAACytoplasmicN/AyesyesNo310.132CYT0-0.200913-00NiCytoplasmic853
PAAZ_ECOLIP77455
paaZ

maoC ydbN

maoC ydbNb1387 JW1382 cytoplasmic protein, Bifunctional PaaZNo transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.CytoplasmicAACytoplasmicIM (predicted-detected)noyesNo330.11CYT0-0.200913-00NiCytoplasmic98
PUR9_ECOLIP15639
purH
b4006 JW3970 cytoplasmic protein, Bifunctional purine biosynthesis PurHCytoplasmicAACytoplasmicN/AyesyesNo270.151CYT0-0.200913-00NiCytoplasmic972
RIBF_ECOLIP0AG40
ribF

yaaC

yaaCb0025 JW0023 cytoplasmic protein, Bifunctional riboflavin kinase/FMN adenylyltransferase RibFCytoplasmicAACytoplasmicN/AyesyesNo650.123CYT0-0.200913-00NiCytoplasmic942
SPOT_ECOLIP0AG24
spoT
b3650 JW3625 cytoplasmic protein, Bifunctional SpoTCytoplasmic (Probable)PMID: 17616600CytoplasmicAACytoplasm CytoplasmicN/AyesyesNo690.124CYT0-0.200913-00NiCytoplasmic88
GLND_ECOLIP27249
glnD
b0167 JW0162 cytoplasmic protein, Bifunctional uridylyltransferase/uridylyl-removing enzyme GlnDCytoplasmicAACytoplasmicN/AyesyesNo210.105CYT0-0.200913-00NiCytoplasmicMembrane8
ADIA_ECOLIP28629
adiA

adi

adib4117 JW5731 cytoplasmic protein, Biodegradative arginine decarboxylase AdiACytoplasmicAACytoplasmCytoplasmicN/AnoyesNo210.114CYT0-0.200913-00NiCytoplasmic1030
BSSR_ECOLIP0AAY1
bssR

yliH

yliHb0836 JW0820 cytoplasmic protein, Biofilm regulator BssRCytoplasmicAACytoplasmicN/AyesyesNo230.138CYT0-0.200913-00NiUnknown251
BSSS_ECOLIP0AB33
bssS

yceP

ycePb1060 JW5152 cytoplasmic protein, Biofilm regulator BssSCytoplasmicAACytoplasmicN/AyesyesNo390.109CYT0-0.200913-00NiUnknown288
BCCP_ECOLIP0ABD8
accB

fabE

fabEb3255 JW3223 cytoplasmic protein, Biotin carboxyl carrier of acetyl-CoA carboxylase AccBCytoplasmicAACytoplasmicN/AyesyesNo500.616CYT0-0.200913-00NiCytoplasmic895
BISC_ECOLIP20099
bisC
b3551 JW5940 cytoplasmic protein, Biotin sulfoxide reductase BisCCytoplasmicAACytoplasmicN/AyesyesNo240.128CYT0-0.200913-00NiPeriplasmic9
BIOB_ECOLIP12996
bioB
b0775 JW0758 cytoplasmic protein, Biotin synthase BioBCytoplasmicAACytoplasmicIM (predicted)yesyesNo330.13CYT0-0.200913-00NiCytoplasmic958
APAH_ECOLIP05637
apaH
b0049 JW0048 cytoplasmic protein, Bis(5'-nucleosyl)-tetraphosphatase ApaHCytoplasmicAACytoplasmicN/AyesyesNo290.105CYT0-0.200913-00NiCytoplasmic927
BLUF_ECOLIP75990
bluF

blrp ycgF

blrp ycgFb1163 JW1150 cytoplasmic protein, Blue light- and temperature-regulated antirepressor BluFCytoplasmicAACytoplasmicN/AnoyesNo490.111CYT0-0.200913-00NiCytoplasmicMembrane720
ILVE_ECOLIP0AB80
ilvE
b3770 JW5606 cytoplasmic protein, Branched-chain-amino-acid aminotransferase IlvECytoplasmicAACytoplasmicN/AyesyesNo390.117CYT0-0.200913-00NiCytoplasmic930
ARCC_ECOLIP37306
arcC

ybcF

ybcFb0521 JW0510 cytoplasmic protein, Carbamate kinase ArcCCytoplasmicAACytoplasm CytoplasmicN/AyesyesNo220.244CYT0-0.200913-00Yn3-14c19/20oCytoplasmic916
ARCL_ECOLIQ46807
yqeA
b2874 JW2842 cytoplasmic protein, Carbamate kinase-like YqeACytoplasmicAACytoplasmicN/AyesyesNo200.148SpI152.26791-00Yn5-10c15/16oCytoplasmic997
HYPE_ECOLIP24193
hypE
b2730 JW2700 cytoplasmic protein, Carbamoyl dehydratase HypECytoplasmicAACytoplasmicN/AyesyesNo340.256CYT0-0.200913-00NiCytoplasmic824
HYPF_ECOLIP30131
hypF

hydA

hydAb2712 JW5433 cytoplasmic protein, Carbamoyltransferase HypFCytoplasmicAACytoplasmicN/AnoyesNo200.113CYT0-0.200913-00NiCytoplasmic873
CSRA_ECOLIP69913
csrA

zfiA

zfiAb2696 JW2666 cytoplasmic protein, Carbon storage regulator CsrACytoplasmicAACytoplasm CytoplasmicN/AnoyesNo370.101CYT0-0.200913-00NiUnknown2
CYNT_ECOLIP0ABE9
cynT
b0339 JW0330 cytoplasmic protein, Carbonic anhydrase 1 CynTCytoplasmicAACytoplasmicN/AnoyesNo460.101CYT0-0.200913-00NiCytoplasmic946
CAN_ECOLIP61517
can

cynT2 yadF

cynT2 yadFb0126 JW0122 cytoplasmic protein, Carbonic anhydrase 2 CanCytoplasmicAACytoplasmicN/AyesyesNo330.123CYT0-0.200913-00NiCytoplasmic947
CMOA_ECOLIP76290
cmoA

yecO

yecOb1870 JW1859 cytoplasmic protein, Carboxy-S-adenosyl-L-methionine synthase CmoACytoplasmicAACytoplasmicN/AyesyesNo570.118CYT0-0.200913-00NiCytoplasmic932
CLSC_ECOLIP75919
clsC

ymdC

ymdCb1046 JW5150 cytoplasmic protein, Cardiolipin synthase C ClsCCytoplasmicAACytoplasmicN/AyesyesNo330.304CYT0-0.200913-00Yn4-12c19/20oCytoplasmicMembrane921
CNTA_ECOLIP0ABR7
yeaW
b1802 JW5294 cytoplasmic protein, Carnitine monooxygenase oxygenase subunit YeaWCytoplasmicAACytoplasmicN/AnoyesNo600.271CYT0-0.200913-00NiCytoplasmic9107
CNTB_ECOLIP76254
yeaX
b1803 JW1792 cytoplasmic protein, Carnitine monooxygenase reductase subunit YeaXCytoplasmicAACytoplasmicN/AnoyesNo580.134CYT0-0.200913-00NiCytoplasmic971
CAIE_ECOLIP39206
caiE
b0035 JW5004 cytoplasmic protein, Carnitine operon CaiECytoplasmicAACytoplasmicN/AyesyesNo270.148CYT0-0.200913-00NiCytoplasmic918
CAID_ECOLIP31551
caiD

yaaL

yaaLb0036 JW0035 cytoplasmic protein, Carnitinyl-CoA dehydratase CaiDCytoplasmicAACytoplasmicN/AyesyesNo260.176CYT0-0.200913-00NiCytoplasmic915
CATE_ECOLIP21179
katE
b1732 JW1721 cytoplasmic protein, Catalase KatETrigger factor-sensitive aggregation.CytoplasmicAACytoplasm CytoplasmicN/ATFyesyesNo480.103CYT0-0.200913-00NiCytoplasmic1043
KATG_ECOLIP13029
katG
b3942 JW3914 cytoplasmic protein, Catalase-peroxidase KatGTrigger factor-sensitive aggregation.CytoplasmicAACytoplasmicN/ATFyesyesNo300.121CYT0-0.200913-00NiCytoplasmic1084
CBRA_ECOLIP31456
cbrA

yidS

yidSb3690 JW5631 cytoplasmic protein, CbrACytoplasmicAACytoplasmicN/AnoyesNo200.273CYT0-0.200913-00NiUnknown234
CEDA_ECOLIP0AE60
cedA

ydjP

ydjPb1731 JW1720 cytoplasmic protein, Cell division activator CedACytoplasmicAACytoplasmicN/AyesyesNo610.153CYT0-0.200913-00NiUnknown2107
SULA_ECOLIP0AFZ5
sulA

sfiA

sfiAb0958 JW0941 cytoplasmic protein, Cell division inhibitor SulACytoplasmic (Probable) SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ.PMID: 12808143CytoplasmicAACytoplasmicN/AyesyesNo400.133CYT0-0.200913-00NiCytoplasmicMembrane1061
ZAPC_ECOLIP75862
zapC

ycbW

ycbWb0946 JW5125 cytoplasmic protein, Cell division ZapCCytoplasmic (Probable)ZapC is a stabilizer of the FtsZ ring.PMID: 21216995 PMID: 21216997CytoplasmicAACytoplasm CytoplasmicN/AyesyesNo360.113CYT0-0.200913-00NiUnknown237
ZAPE_ECOLIP64612
zapE

yhcM

yhcMb3232 JW3201 cytoplasmic protein, Cell division ZapECytoplasmicAACytoplasm CytoplasmicN/AyesyesNo220.106CYT0-0.200913-00NiCytoplasmic931
YBBN_ECOLIP77395
ybbN
b0492 JW5067 cytoplasmic protein, Chaperedoxin YbbNCytoplasmicAACytoplasmicN/AnoyesNo500.128CYT0-0.200913-00NiCytoplasmic975
CBPM_ECOLIP63264
cbpM

yccD

yccDb0999 JW0984 cytoplasmic protein, Chaperone modulator CbpMCytoplasmicAACytoplasmicN/AyesyesNo220.105CYT0-0.200913-00NiUnknown235
NAPD_ECOLIP0A9I5
napD

yojF

yojFb2207 JW2195 cytoplasmic protein, Chaperone NapDCytoplasmic (Experimental)NapD is a Tat signal peptide-binding chaperone involved in biosynthesis of the Tat-dependent nitrate reductase NapA. PMID: 17074894PMID: 17074894CytoplasmicA