STEPdb: Sub-cellular Topologies of E.coli Polypeptides.

View: Type three secretion proteins (T3SS) in E.coli EPEC (O127:H6) (encoded on the LEE locus)  Export to Excel  Export to HTML  Export to XML  Export to CSV  

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Type three secretion proteins (T3SS) in E.coli EPEC (O127:H6) (encoded on the LEE locus)


Accession
(Uniprot)
E.coliEPEC
(O127:H6)
Common name
Gene name
E.coliEPEC
(O127:H6)
Protein name
E.coliEPEC
(O127:H6)
Protein
Length
Structural
domains
Position
Post-Translational
Modifications
(PTMs)
Secretion
Mechanism
Effect of
deletion mutation
on secretion
Effect on
pedestal
formation
/virulence
Subcellular
Location
Functional
Domains
(PFAM)
Known
Interactions
in Bacterium
Known Function
References
B7UM95
SctW
sepL
SepL
351
N/A
Gate-keeper
N/A
No
-/Translocators
-/-
C (weak interaction IM)
SepD
Switches translocation. Single protein mediating effects of YopN and TyeA; internal interaction between YopN- and TyeA-like moieties; also a translocated effector?
B7UMA7
SctV
escV
EscV
675
7TM
Gate
N/A
Sec?
-
-/-
Inner Membrane
EscN
C-terminal cytoplasmic domain might play a role in gene regulation.
B7UMB8
SctU
escU
EscU
345
N/A
Export Apparatus/autoprotease
cleaved at NPTH motif
Sec?
-
-
Inner Membrane
EscR
T3S
B7UMB9
SctT
escT
EscT
258
N/A
Export Apparatus/IM Component
N/A
Sec?
-
-/-
Inner Membrane
 
T3S
B7UMC0
SctS
escS
EscS
89
N/A
Export Apparatus/IM Component
N/A
Sec?
-
-/- EscR
Inner Membrane
EscR
T3S
B7UMC1
SctR
escR
EscR
217
N/A
Export Apparatus/IM Component
N/A
Sec?
-
-/-
Inner Membrane
EscR EscS EscU EspD SepZ
T3S
B7UMA4
SctP
orf16
EscP
138
N/A
-
N/A
T3S
±/Translocators
±/±
N/A
CesT EscF EscI EscU Orf16
N/A
B7UMA6
SctN
escN
EscN
446
cytoplasmic
Export Apparatus
N/A
No
-
-/-
N/A
CesT5 EscL EscQ EscV Tir5
ATPase (walker box motif). Forms hexameric complex.
B7UMC2
SctL
orf5
EscL
231
N/A
sorting platform/peripheral stalk
N/A
No
-
-/-
N/A
N/A
EscN EscQ EspA
T3S
B7UMC3
SctK
orf4
EscK
199
N/A
sorting platform
N/A
No
-
-/-
Cytoplasm
N/A
 
T3S
B7UMB1
SctJ
escJ
EscJ
190
N/A
Basal Body/Smaller inner ring protein
Palmitoylated at C19 (by analogy to PrgK PMID: 15931226)
Sec, Lipoprotein
-
-/-
Inner Membrane
EscJ
Essential molecular platform for T3SS assembly
B7UMB0
SctI
rorf8
EscI
142
N/A
Inner rod protein.
N/A
T3S?
-
-/-
N/A
EscU rOrf3
T3S; Inner rod protein assists in secretin insertion into OM.
B7UM90
SctF
escF
EscF
73
N/A
Needle filament
N/A
T3S
-
-/-
N/A
EscJ
Major Needle component
B7UM93
SctE
espD
EspD
380
N/A
Translocon
N/A
T3S
+
-/-
N/A
EspD
Translocator
B7UM96
SctD
escD
EscD
380
N/A
Basal Body/Larger inner rod ring protein
N/A
Sec?
-
-/-
N/A
N/A
 
EscD and EscJ form ring-like structure in inner membrane; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling.
 
B7UMB3
SctC
escC
EscC
512
N/A
Basal Body Secreting/outer ring
N/A
Sec?
--/-
N/A
N/A
 
T3SS basal body; T3SS OM ring.
Q05129
SctB
espB
EspB
321
N/A
Translocon
N/A
T3S
+
-/-
N/A
CesAB2
Translocator. Binds to myosins that interact with actin filaments and mediate essential processes (e.g.microvillus formation ,phagocytosis). Inhibits the interaction of myosins with actin PMID: 18078690.
B7UM94
SctA
espA
EspA
192
N/A
needle tip
N/A
T3S
+
-/-
Bacterial Surface
CesAB
Translocator. Half-flagellin model; dimerizes before polymerizes; lacks D0 and D3 domains.
B7UMC9
N/A
rorf1
N/A
 
N/A
N/A
N/A
N/A
+
+/+
N/A
N/A
 
SdiA-regulated domain (NHLrepeats).
 
B7UMC8
N/A
rorf2
EspG1
398
N/A
N/A
N/A
T3S
+
+/+
Host Cytoplasm
 
Translocated effector. Disassemble microtubules to induce RhoGTPase-mediated cytokeletal rearrangements that promote bacterial attachment, entry, or movement within the cytoplasm PMID: 15972534. Activating the host cell protease calpain.
B7UMC6
N/A
ler
Ler
129
N/A
N/A
N/A
No
No expression
-/-
Cytoplasm
HNS StpA
Positive regulator of T3SS
B7UMC5
N/A
orf2
EscE
72
N/A
N/A
N/A
N/A
N/A
Orf29
Cytoplasm
N/A
SepL
-
B7UMC4
N/A
orf3
CesAB
107
N/A
Chaperone (translocators)
N/A
No
±EspA, EspB
-/-
Cytoplasm
N/A
EspA3, 1, 2 EspB2
Chaperone for EspA and EspB
B7UMB7
N/A
rorf3
etgA
152
N/A
N/A
N/A
Sec
±
±/±
N/A
EscI
-
B7UMB6
N/A
orf10
grlR
123
N/A
N/A
N/A
No
+
+/++
Cytoplasm
N/A
 
Negative regulator of TTSS
 
B7UMB5
N/A
orf11
grlA
137
N/A
N/A
N/A
No
No expression
-/-
Cytoplasm
N/A
 
Negative regulator TTSS.
 
B7UMB4
N/A
cesD
CesD
151
N/A
Chaperones
N/A
No
-/EspD
±/-
Cytoplasm, PIM
EscD2 EspD4
-
B7UMB2
N/A
rorf6
SepD
151
N/A
Gate-keeper
N/A
No
-/(translocators are not secreted)
-/-
N/A
N/A
SepL
Switches translocation/effector secretion.
 
B7UMA9
N/A
espZ/sepZ
EspZ/SepZ
98
2TM
N/A
N/A
T3S
+
+/±
N/A
N/A
EscR
Translocated effector; Secreted effector; Contributes to host cell survival mechanisms PMID: 31.
 
B7UMA8
N/A
orf12
Mpc/CesL
117
cytoplasmic
N/A
N/A
No
-
-/-
N/A
N/A
 
Interacts with Ler-suppresses LEE expression PMID: 21839744.
 
B7UMA5
N/A
orf15
EscO
125
N/A
Central stalk
N/A
No
-
-/-
N/A
N/A
 
N/A
 
B7UMA3
N/A
sepQ
EscQ
305
N/A
sorting platform/C-ring homolog
N/A
No
-
-/-
N/A
N/A
 
Forms ring within basal body PMID: 20477867; interacts with EscL PMID: 11349076.
 
N/A
N/A
orf18
N/A
 
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
 
N/A
 
B7UMA2
N/A
N/A
EspH
168
N/A
N/A
N/A
T3S
+
+/++
N/A
N/A
 
Translocated effector.
 
B7UMA1
N/A
N/A
CesF
120
N/A
N/A
N/A
No
±/EspF?
+/+
Cytoplasm
EspF
Chaperone for EspF.
 
B7UMA0
N/A
orf19
Map
203
N/A
Effector
N/A
T3S
+
+/++
N/A
CesT
Mitochondrial dysfunction PMID: 16548893. Induces transient filopodia formation PMID: 19046338.
B7UM99
N/A
tir
Tir
550
N/A
Effector
pY474 by host kinases; pY454 by host kinases PMID: 15220932; pS436, pS437 by host kinases PMID: 16954405.
T3S
+
-/-
Host cytoplasmic Membrane
CesT5 EscN5
N-terminal and C-terminal domains remain intracellular and interact with numerous signalling, adapter and cytoskeletal proteins. Function dependent on its phosphorylation sites, a GTPase activating protein (GAP)-like motif and a polyproline region. Phosphorylation affects oligomerization PMID: 12738766.
P21244
N/A
cesT
CesT
156
N/A
Chaperone
N/A
No
±/Tir
-/-
Cytoplasm
CesT2 EscN5 EspF2 GapA Map2 SepL8 Tir5, 6, 7
Chaperone of Tir and Map
P19809
N/A
eae
Intimin
939
N/A
N/A
N/A
Self/Autotransporter?
+
-/-
N/A
N/A
 
Adhesin .
B7UM91
N/A
orf27
cesD2
135
N/A
N/A
N/A
No
±/EspD?
+/±
Cytoplasm
EscD2 EspD4
Chaperone of EspD. LcrR will bind YopB.
 
B7UM89
N/A
orf29
EscG
92
N/A
chaperone of EscF
N/A
No
-
-/-
N/A
N/A
 
Functionally important conserved residues can be identified by scrutiny of homologues, Needle component like SsaH? Is that why it binds EspA?
 
B7UM88
N/A
espF
EspF
206
N/A
N/A
N/A
T3S
+
+/+
N/A
CesF
Translocated effector. Mitochondrial dysfunction PMID: 15533930 PMID: 15469437. Microvilli effacement PMID: 15491358. TJ disruption, apoptosis PMID: 16548889. Epithelial transporter inhibition PMID: 16446436. Antiphagocytosis. Membrane remodeling PMID: 15664974. Actin-pedestal maturation. Nucleolus.
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