STEPdb: Sub-cellular Topologies of E.coli Polypeptides.

View: Type three secretion proteins (T3SS) in E.coli EPEC (O127:H6) (lee locus)  Export to Excel  Export to HTML  Export to XML  Export to CSV  

 Search
    

Type three secretion proteins (T3SS) in E.coli EPEC (O127:H6) (lee locus)


Protein name
E.coliEPEC
(O127:H6)
Previous names
E.coliEPEC
(O127:H6)
Accession
(Uniprot)
E.coliEPEC
(O127:H6)
Common
nomenclature
name
Gene name
E.coliEPEC
(O127:H6)
Molecular
weight (Da)
Protein
Length
(amino acids)
Structural
domains
Position
Stochiometry
Subunit size (kDa)
Post-Translational
Modifications
(PTMs)
Secretion
Mechanism
Effect of deletion mutation
on secretion ("-" = no secretion
of any substrates)
Effect on pedestal formation
/virulence ("-" = no pedestals,
no virulence; "+" = induced
virulence and pedestal formation)
Subcellular
Location
Functional
Domains
(PFAM)
Known
Interactions
between T3S
components
Known Function
References
SepL
/
B7UM95
SctW
sepL
39
351
3 helical domains
Gatekeeper/Affinity switch
unknown
unknown
N/A
T3S
Translocators not secreted, Effectors oversecreted
-
Cytoplasm (weak interaction IM)
CesT EscV Secreted proteins and their chaperones SepD
Receptor at the membrane for translocation; Once absent the translocation switches to effectors
EscV
SepA
B7UMA7
SctV
escV
75
675
7 TM; large cytoplasmic domain
Major component, external to channel
9
676,8
N/A
Sec
-
-
Inner Membrane
EscJ Secreted proteins and their chaperones SepL
Receptor at the membrane for translocation; Translocation channel
EscU
/
B7UMB8
SctU
escU
39
345
3 TM; large cytoplasmic domain
Minor component, external to channel
1 to 2
39.2-78.4
cleaved at NPTH motif
Sec
-
-
Inner Membrane
EscI EscP EscR
Export apparatus; Switch mechanism from early to middle substrates
EscT
/
B7UMB9
SctT
escT
28
258
5 TM
Translocase channel
1
28,7
N/A
Sec
-
-
Inner Membrane
EscR EscS EscT
Export apparatus
EscS
/
B7UMC0
SctS
escS
9
89
2 TM
Translocase channel
1
9,9
N/A
Sec
-
-
Inner Membrane
EscR EscT
Export apparatus
EscR
/
B7UMC1
SctR
escR
24
217
4 TM
Translocase channel
5
121,5
N/A
Sec
-
-
Inner Membrane
EscI EscR EscS EscT EscU
Export apparatus
EscP
/
B7UMA4
SctP
orf16
16
138
C-terminal conserved T3S4 domain (type III secretion and substrate specificity switch) that interacts with SctU C-terminus
Molecular ruler
unknown
unknown
N/A
T3S
Less translocators
Not affected
Secretory protein
CesT EscF EscI EscU Orf16
Ruler for filament length; Switch mechanism from early to middle substrates
EscN
SepB
B7UMA6
SctN
escN
48
446
cytoplasmic; residues 103?371 contain a mixed ?/? Rossmann fold; highly helical
Hexameric ring-structure ATPase
6
293,1
N/A
No
-
-
Cytoplasm (weak interaction IM)
EscL EscO EscQ EscV Secreted proteins and their chaperones
ATPase (walker box motif). Forms hexameric complex
EscL
/
B7UMC2
SctL
orf5
27
231
helical protein
External stator connecting ATPase and Cytoplasmic ring
12
326,2
N/A
No
-
-
Cytoplasm (weak interaction IM)
N/A
EscN EscO EscQ EscV EspA
Negative regulator of ATPase
EscK
/
B7UMC3
SctK
orf4
23
199
N/A
Connector of Pods with SctD
6
140,8
N/A
No
-
-
Cytoplasm (weak interaction IM)
N/A
EscD EscQ
Connector of Pods with SctD
EscJ
/
B7UMB1
SctJ
escJ
20
190
1 large TM, periplasm-facing lipoprotein
Lipoprotein ring component
24
498,2
Palmitoylated at C19 (by analogy to PrgK PMID: 15931226)
Sec, Lipoprotein
-
-
Inner Membrane
EscD EscI EscJ EscV
IM ring
EscI
/
B7UMB0
SctI
rorf8
15
142
peripolasmic protein
Inner rod
3
45,9
N/A
T3S
-
-
Periplasm
EscC EscI EscP EscR EscT EscU EtgA
Inner rod protein assists in secretin insertion into OM
EscF
/
B7UM90
SctF
escF
8
73
helical protein
Needle component
120
974
N/A
T3S
-
-
Secretory protein
EscE EscF EscG EscP
Major Needle component
EspD
/
B7UM93
SctE
espD
39
380
2 TM, mainly hydrophylic
Translocon pore protein
8
316
N/A
T3S
Not affected
-
Host plasma membrane
CesD2 EspA EspB EspC EspD
Translocator, pore forming protein
EscD
/
B7UM96
SctD
escD
45
406
1 TM
Major IM ring component
24
1087,2
N/A
Sec
-
-
Inner Membrane
N/A
EscC EscJ EscK EscQ
EscD and EscJ form ring-like structure in inner membrane; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling.
EscC
/
B7UMB3
SctC
escC
56
512
N-terminal periplasmic domain; secretin family protein
Secretin
12 to 14
674,4
N/A
Sec
-
-
Outer Membrane
EscD EscI EscO
T3SS basal body and forms OM ring
EspB
eaeB
Q05129
SctB
espB
33
321
mainly hydrophylic and helical
Translocon pore protein
8
264,8
N/A
T3S
Not affected
-
Host plasma membrane
CesAB CesD EscN EscV EspA EspD SepL
Translocator, pore forming protein. Binds to myosins that interact with actin filaments and mediate essential processes (e.g.microvillus formation ,phagocytosis). Inhibits the interaction of myosins with actin PMID: 18078690.
EspA
/
B7UM94
SctA
espA
20
192
mainly helical
Filament protein
28 per turn
574
N/A
T3S
Not affected
-
Secretory protein
CesAB EscL EscN EscV EspB EspD SepL
Translocator. Half-flagellin model; dimerizes before polymerizes; lacks D0 and D3 domains.
N/A
/
B7UMC9
N/A
rorf1
31
272
N/A
N/A
unknown
unknown
N/A
N/A
unknown
+
N/A
N/A
 
SdiA-regulated domain (NHLrepeats). Unknown Function.
 
EspG
rorf2
B7UMC8
N/A
espG
43
398
cytoplasmic
Effector
more than injectisomes
unknown
N/A
T3S
Not affected
+
Host Cytoplasm
CesT
Translocated effector. Disassemble microtubules to induce RhoGTPase-mediated cytokeletal rearrangements that promote bacterial attachment, entry, or movement within the cytoplasm PMID: 15972534. Activating the host cell protease calpain.
Ler
orf1
B7UMC6
N/A
ler
15
129
cytoplasmic
Global Regulator
more than injectisomes
unknown
N/A
No
No expression
-
Cytoplasm
HNS Mcp/CesL StpA
Positive regulator of T3SS
EscE
/
B7UMC5
N/A
orf2
8
72
cytoplasmic
Chaperone of EscF
more than injectisomes
unknown
N/A
No
-
-
Cytoplasm
N/A
SepL
One of the chaperones of EscF
CesAB
/
B7UMC4
N/A
orf3
12
107
cytoplasmic
Chaperone (translocators)
more than injectisomes
unknown
N/A
No
No EspA, less EspB
-
Cytoplasm
N/A
EscN EscV EspA EspB SepL
Chaperone for EspA and EspB
EtgA
/
B7UMB7
N/A
rorf3
17
152
peripolasmic
Muramidase
unknown
unknown
N/A
Sec
-
+
Peptidoglycan
EscI
Muramidase that degrades peptidoglycan
GrlR
/
B7UMB6
N/A
orf10
13
123
cytoplasmic
Negative regulator
unknown
unknown
N/A
No
Not affected
+
Cytoplasm
N/A
GrlA
Negative regulator TTSS; avoiding GrlA binding to the LEE1 promoter and thus repressing ler expression
GrlA
/
B7UMB5
N/A
orf11
16
137
cytoplasmic
Positive regulator
unknown
unknown
N/A
No
No expression
-
Cytoplasm
N/A
GrlR
Positive regulator TTSS
CesD
/
B7UMB4
N/A
cesD
17
151
cytoplasmic
Subunit 2 of SctW chaperone heterodimer
1 per SctW
unknown
N/A
No
Translocators not secreted, Effectors oversecreted
-
Cytoplasm
EspB EspD
Chaperone for EspB and EspD
SepD
/
B7UMB2
N/A
rorf6
17
151
cytoplasmic
Subunit 1 of SctW chaperone heterodimer
1 per SctW
unknown
N/A
No
Translocators not secreted, Effectors oversecreted
-
Cytoplasm
N/A
CesL SepL
Chaperone of SepL together with CesL and regulator of switch mechanism
 
EspZ/SepZ
/
B7UMA9
N/A
espZ/sepZ
9
98
2TM
Effector
more than injectisomes
unknown
N/A
T3S
Not affected
+
Host Cytoplasm
N/A
EscR
Translocated effector; Contributes to host cell survival mechanisms
 
Mpc/CesL
/
B7UMA8
N/A
orf12
13
117
cytoplasmic
Chaperone (effectors)
1 per SctW
unknown
N/A
No
-
-
Cytoplasm
N/A
EscO Ler SepD SepL
Chaperone of SepL together with SepD and regulator of switch mechanism
EscO
/
B7UMA5
N/A
orf15
14
125
cytoplasmic
Central stalk, inserting in ATPase ring
1
14,7
N/A
No
-
-
Cytoplasm (weak interaction IM)
N/A
EscC EscG EscL EscN EscV Mcp/CesL
central stalk attached to C-terminus of EscV; stabilizes the EscN hexamer and stimulates its ATPase activity
EscQ
SepQ
B7UMA3
N/A
escQ
34
305
cytoplasmic
Component of 6 Pod assembly
12
419,6
N/A
No
-
-
Cytoplasm (weak interaction IM)
N/A
EscD EscK EscL EscN
Forms ring within basal body
EspH
orf18
B7UMA2
N/A
espH
19
168
secreted
Effector
more than injectisomes
unknown
N/A
T3S
Not affected
+
Host Cytoplasm
N/A
CesT
Translocated effector
CesF
rorf10
B7UMA1
N/A
cesF
14
120
cytoplasmic
Chaperone (effectors)
more than injectisomes
unknown
N/A
No
Not affected
+
Cytoplasm
EspF
Chaperone for EspF
 
Map
/
B7UMA0
N/A
orf19
22
203
secreted
Effector
more than injectisomes
unknown
N/A
T3S
Not affected
+
Host Cytoplasm
CesT
Mitochondrial dysfunction; Induces transient filopodia formation
Tir
Hp90
B7UM99
N/A
tir
56
550
secreted
Effector
more than injectisomes
unknown
pY474 by host kinases; pY454 by host kinases PMID: 15220932; pS436, pS437 by host kinases PMID: 16954405.
T3S
Not affected
-
Host cytoplasmic membrane
CesT EscN
N-terminal and C-terminal domains remain intracellular and interact with numerous signalling, adapter and cytoskeletal proteins. Function dependent on its phosphorylation sites, a GTPase activating protein (GAP)-like motif and a polyproline region. Phosphorylation affects oligomerization.
CesT
orfU
P21244
N/A
cesT
17
156
cytoplasmic
Chaperone (effectors)
more than injectisomes
unknown
N/A
No
Not affected
-
Cytoplasm
CesT EscN EspF EspG EspH EspZ GapA Map NleA NleF NleG NleH NleH2 SepL Tir
Chaperone of Tir and Map
Intimin
eaeA
P19809
N/A
eae
102
939
secreted
Attaching and effacing protein
more than injectisomes
unknown
N/A
T3S
Not affected
-
Bacterial outer membrane
N/A
Tir
Adhesin on the bacterial membrane
CesD2
/
B7UM91
N/A
orf27
15
135
cytoplasmic
Chaperone (translocators)
more than injectisomes
unknown
N/A
No
Less EspD
Not affected
Cytoplasm
EspB EspD
Chaperone of EspD. Homologous LcrR will bind YopB.
 
EscG
/
B7UM89
N/A
orf29
10
92
cytoplasmic
Chaperone of EscF
more than injectisomes
unknown
N/A
No
-
-
Cytoplasm
N/A
EscE EscF EscO
One of the chaperones of EscF
EspF
orfd4
B7UM88
N/A
espF
20
206
secreted
Effector
more than injectisomes
unknown
N/A
T3S
Not affected
+
Host Cytoplasm
CesF CesT
Translocated effector. Mitochondrial dysfunction. Microvilli effacement. TJ disruption, apoptosis. Epithelial transporter inhibition. Antiphagocytosis. Membrane remodeling. Actin-pedestal maturation. Nucleolus.
Records 1 to 41 of 41     
Page Size 

 

 ©2017 Copyright KU Leuven and FORTH/ICE-HT. Last Update: February 2019.