entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocibernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubilitynterm_cterm
SGRT_ECOLIC1P5Z7sgrTb4662 JW0068.1 cytoplasmic peripheral IM protein, Putative inhibitor of glucose uptake transporter SgrT Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Putative inhibitor of glucose uptake transporter sgrT. SgrT protein expresssion is inducted by ??-methylglucoside.[18042713]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/A-noyesN230.105CYT0-0.200913-00NiUnknown2
YOBI_ECOLIC1P604yobIb4677 JW1850.1 cytoplasmic peripheral IM protein, YobISmall membrane protein detected experimentallyCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/A-noyesN120.1CYT0-0.200913-00NiUnknown2
AZUC_ECOLIC1P605azuCb4663 JW1891.1 cytoplasmic peripheral IM protein, AzuCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Small protein detected experimentally. AzuC-SPA was most abundant in the inner membrane fraction (77%) indicating this protein is bound to the cytoplasmic side of the inner membrane.[19121005] [21778229]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane -noyesN270.099CYT0-0.200913-00NiUnknown2
YSHB_ECOLIC1P620yshBb4686 JW3839.1 cytoplasmic peripheral IM protein, YshBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Amphipaseek predicts N-terminal amphipathic helix (1-10). Amphipaseek predicts N-terminal amphipathic helix (1-10). Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/A-noyesN250.194CYT0-0.200913-00NiUnknown2
6PGD_ECOLIP00350gndb2029 JW2011 cytoplasmic peripheral IM protein, 6-phosphogluconate dehydrogenase, decarboxylating GndCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN200.182CYT0-0.200913-00NiCytoplasmic890
FRDA_ECOLIP00363frdAb4154 JW4115 cytoplasmic peripheral IM protein, Fumarate reductase flavosubunit FrdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)FrdA and FrdB Fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). [PMC323229] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [PMC323229] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN280.271SpI271.50227-00Yn8-18c27/28oCytoplasmicMembrane950
YIDD_ECOLIP0A8C8yidDb4557 JW3682 cytoplasmic peripheral IM protein, Putative insertion efficiency factor YidDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)[21803992]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicnoyesN420.11CYT0-0.200913-00NiUnknown2
DHNA_ECOLIP00393ndhb1109 JW1095 cytoplasmic peripheral IM protein, NADH dehydrogenase NdhCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains amphipathic C-terminal anchor (390-406). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20933494] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell membraneCytoplasmicyesyesN220.114CYT0-0.200913-00NiCytoplasmicMembrane1035
RIR1_ECOLIP00452nrdAdnaFb2234 JW2228 cytoplasmic peripheral IM protein, Ribonucleoside-diphosphate reductase 1 subunit alpha NrdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 [9395490] Disulfide bridges between 225 and 462 [9395490] Disulfide bridges between 225 and 462 [9395490][23230279] [9395490]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN400.104CYT0-0.200913-00NiCytoplasmic963
AAT_ECOLIP00509aspCb0928 JW0911 cytoplasmic peripheral IM protein, Aspartate aminotransferase AspCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [3521591]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmic (Experimental)yesyesN230.126CYT0-0.200913-00NiCytoplasmic10
AK1H_ECOLIP00561thrAthrA1 thrA2b0002 JW0001 cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 1 ThrACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [4562989] [354697] [7003595] [6777249][15911532] [4562989] [354697] [7003595] [6777249]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmic (Experimental)TFyesyesN150.142CYT0-0.200913-00NiCytoplasmic8
GNSA_ECOLIP0AC92gnsAyccLb4517 JW0976 cytoplasmic peripheral IM protein, GnsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN500.102CYT0-0.200913-00NiUnknown2
AK2H_ECOLIP00562metLmetMb3940 JW3911 cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 2 MetLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN240.117CYT0-0.200913-00NiCytoplasmicMembrane9
RPOD_ECOLIP00579rpoDaltb3067 JW3039 cytoplasmic nucleoid peripheral IM protein, RNA polymerase sigma factor RpoD Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicTFyesyesN260.104CYT0-0.200913-00NiCytoplasmic992
DPO1_ECOLIP00582polAresAb3863 JW3835 cytoplasmic nucleoid peripheral IM protein, DNA polymerase I PolACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicnoyesN590.268CYT0-0.200913-00NiCytoplasmic976
YIFE_ECOLIP0ADN2yifEb3764 JW3737 cytoplasmic peripheral IM protein, UPF0438 YifECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN440.105CYT0-0.200913-00NiUnknown2
ZAPB_ECOLIP0AF36zapByiiUb3928 JW3899 cytoplasmic peripheral IM protein, Cell division ZapBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[20487275] [18394147]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN190.122CYT0-0.200913-00NiUnknown281
YGGL_ECOLIP38521yggLb2959 JW2926 cytoplasmic peripheral IM protein, YggLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN290.118CYT0-0.200913-00NiCytoplasmic8129
CAPP_ECOLIP00864ppcglub3956 JW3928 cytoplasmic peripheral IM protein, Phosphoenolpyruvate carboxylase PpcCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN480.115CYT0-0.200913-00NiCytoplasmic8
TRPE_ECOLIP00895trpEb1264 JW1256 cytoplasmic peripheral IM protein, Anthranilate synthase component 1 TrpECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN190.11CYT0-0.200913-00NiCytoplasmic1066
TRPGD_ECOLIP00904trpGDtrpDb1263 JW1255 cytoplasmic peripheral IM protein, Bifunctional TrpGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Could not obtain any evidence for membrane binding (F1) Peripheral IM Cytoplasmic side. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [4594441] [6283099][19766000] [15911532] [4594441] [6283099]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)yesyesN190.166CYT0-0.200913-00NiCytoplasmic922
YAEH_ECOLIP62768yaeHb0163 JW0159 cytoplasmic peripheral IM protein, UPF0325 YaeHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN260.108CYT0-0.200913-00NiCytoplasmic893
YAAY_ECOLIP75620yaaYb0024 JW5003 cytoplasmic peripheral IM protein, YaaYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/AMembrane anchorednoyesN430.125CYT0-0.200913-10NiCytoplasmicMembrane9
SYA_ECOLIP00957alaSlovBb2697 JW2667 cytoplasmic nucleoid peripheral IM protein, Alanine--tRNA ligase AlaSCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmCytoplasmicTFnoyesN260.129CYT0-0.200913-00NiCytoplasmic957
SYM_ECOLIP00959metGb2114 JW2101 cytoplasmic peripheral IM protein, Methionine--tRNA ligase MetGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicTFyesyesN200.115CYT0-0.200913-00NiCytoplasmic1049
SYGA_ECOLIP00960glyQglyS(A)b3560 JW3531 cytoplasmic peripheral IM protein, Glycine--tRNA ligase alpha subunit GlyQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicTFnoyesN570.112CYT0-0.200913-00NiCytoplasmic959
SYGB_ECOLIP00961glySglyS(B)b3559 JW3530 cytoplasmic peripheral IM protein, Glycine--tRNA ligase beta subunit GlySCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN650.169CYT0-0.200913-00NiCytoplasmic1077
CARB_ECOLIP00968carBpyrAb0033 JW0031 cytoplasmic peripheral IM protein, Carbamoyl-phosphate synthase large chain CarBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN240.228CYT0-0.200913-00Yn9-16c21/22oUnknown4
DNAA_ECOLIP03004dnaAb3702 JW3679 cytoplasmic nucleoid peripheral IM protein, Chromosomal replication initiator DnaACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)C-terminal amphiphilic helix (355-370). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10660626] [23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmCytoplasmicnoyesN320.146CYT0-0.200913-00NiCytoplasmic1038
LACI_ECOLIP03023lacIb0345 JW0336 cytoplasmic peripheral IM protein, Lactose operon repressor LacICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Regulates lac operon transcription when in homotetrameric form.[21936772] [15863616] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN330.136CYT0-0.200913-00NiCytoplasmic1032
GUAA_ECOLIP04079guaAb2507 JW2491 cytoplasmic peripheral IM protein, GMP synthase GuaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN450.122CYT0-0.200913-00NiCytoplasmic9
AMPN_ECOLIP04825pepNb0932 JW0915 cytoplasmic peripheral IM protein, Aminopeptidase PepNCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous peptidases are membrane associated in mammals, some data propose that the enzyme may associate with the E.coli membrane. It is structurally classified with <a href="http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/enzymes/GetPage.pl?ec_number=3.4.11.2">EC 3.4.11.2</a> membrane alanyl aminopeptidases. However, the extent of membrane association needs further analysis as it is generally soluble in the cytoplasmic fraction of E.coli and other bacteria. [323010] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][323010] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmic (Experimental)yesyesN500.108CYT0-0.200913-00NiCytoplasmicMembrane10101
RBSA_ECOLIP04983rbsAb3749 JW3728 cytoplasmic peripheral IM protein, Ribose import ATP-binding RbsA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN350.548CYT0-0.200913-00NiCytoplasmicMembrane1049
FUMC_ECOLIP05042fumCb1611 JW1603 cytoplasmic peripheral IM protein, Fumarate hydratase class II FumCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN570.12CYT0-0.200913-00NiCytoplasmic1064
PNP_ECOLIP05055pnpb3164 JW5851 cytoplasmic peripheral IM protein, Polyribonucleotide nucleotidyltransferase PnpCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Polynucleotide phosphorylase (PNPase) or Polyribonucleotide nucleotidyltransferase Is a cytoplasmic enzyme that has also been isolated in association with the inner membrane (Stenberg et al., 2005; Maddalo et al., 2011). It is not known if association is direct. The protein has weak hydrophobic patches and three regions with weak predictions from Phobius for membrane interaction. Membrane association may be via binding to RNAaseE with which PNPase interacts as part of the multienzyme complex known as the RNA degradosome. RNAseE has been shown to bind to membranes (Khemici et al., 2008). The interaction of RNase E with PNPase , brings together two enzymes important in the degradation of mRNA. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][16079137] [23230279] [24438330]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicTFyesyesN300.323CYT0-0.200913-00NiCytoplasmic1074
PTHA_ECOLIP05706srlBgutBb2704 JW2673 cytoplasmic peripheral IM protein, PTS system glucitol/sorbitol-specific EIIA component SrlBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)PTS system glucitol/sorbitol-specific EIIA component.The hydropathic profile of the Enzyme IIgut revealed 7 or 8 long hydrophobic segments which may traverse the cell membrane as alpha-helices as well as 2 or 4 short strongly hydrophobic stretches which may traverse the membrane as beta-structure [3553176].[2824435] [3553176]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN350.143CYT0-0.200913-00NiCytoplasmic8115
ILVD_ECOLIP05791ilvDb3771 JW5605 cytoplasmic peripheral IM protein, Dihydroxy-acid dehydratase IlvDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN540.166CYT0-0.200913-00NiCytoplasmic925
ILVC_ECOLIP05793ilvCb3774 JW3747 cytoplasmic peripheral IM protein, Ketol-acid reductoisomerase IlvCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN290.107CYT0-0.200913-00NiCytoplasmic1097
BGLR_ECOLIP05804uidAgurA gusAb1617 JW1609 cytoplasmic peripheral IM protein, Beta-glucuronidase UidACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN590.12CYT0-0.200913-00NiCytoplasmic846
DLD_ECOLIP06149dldb2133 JW2121 cytoplasmic peripheral IM protein, Quinone-dependent D-lactate dehydrogenase DldCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Requires phospholipid for maximal activity. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN330.121CYT0-0.200913-00NiCytoplasmicMembrane1078
BTUD_ECOLIP06611btuDb1709 JW1699 cytoplasmic peripheral IM protein, Vitamin B12 import ATP-binding BtuD Vitamin B12 import ATP-binding protein btuD.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN260.195CYT0-0.200913-00NiCytoplasmicMembrane109
ERA_ECOLIP06616erarbaA sdgEb2566 JW2550 ribosomal cytoplasmic peripheral IM protein, GTPase Era Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Assembly cofactor of ribosome; associate with the 30S subunit and 16S rRNA. YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][8282709] [17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasm CytoplasmicyesyesN410.127CYT0-0.200913-00NiCytoplasmicMembrane1092
ODP2_ECOLIP06959aceFb0115 JW0111 cytoplasmic peripheral IM protein, Dihydroyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex AceFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15911532] [24742683] [15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFnoyesN520.106CYT0-0.200913-00NiCytoplasmic1095
FLIM_ECOLIP06974fliMcheC2 fla AII fla QIIb1945 JW1929 Flagellar cytoplasmic peripheral IM protein, motor switch FliM Flaggelar C-ring proteins - FliG, FliM, and FliN.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane; Peripheral membrane protein Bacterial flagellum basal bodyCytoplasmicflagellumnoyesN100.109CYT0-0.200913-00NiCytoplasmicMembrane1057
HIS7_ECOLIP06987hisBb2022 JW2004 cytoplasmic peripheral IM protein, Histidine biosynthesis bifunctional HisB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN240.105CYT0-0.200913-00NiCytoplasmic1066
RSMA_ECOLIP06992rsmAksgAb0051 JW0050 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase A RsmACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; Overex<x>pression of RsmA suppresses defects in mutant of era. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN330.128CYT0-0.200913-00NiCytoplasmic10
MALT_ECOLIP06993malTmalAb3418 JW3381 cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator MalTCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the MalK peripheral ATPase of the maltose transporter. Trigger factor-sensitive aggregation. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). [15063852] [23138451] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicTFyesyesN360.12CYT0-0.200913-00NiUnknown78
PLDB_ECOLIP07000pldBb3825 JW5584 cytoplasmic peripheral IM protein, Lysophospholipase L2 PldBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Gene and characteristics of deduced amino acid sequence of lysophospholipase L2. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN340.104CYT0-0.200913-00NiCytoplasmicMembrane736
POXB_ECOLIP07003poxBb0871 JW0855 cytoplasmic peripheral IM protein, Pyruvate dehydrogenase PoxBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Is one of several membrane-associated form of the enzyme has a tendency to self-aggregate,is flavo protein dehydrogenases that feed electrons in to the high lysurfacelabile,and manifests. Pyruvate oxidase is a membrane-associated flavoprotein dehydrogenase which is greatly activated by lipids and detergents. The carboxyl-terminal region of the protein has been shown to play a critical role in the interaction with lipids.[2663858]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane; Peripheral membrane proteinCytoplasmicyesyesN620.106CYT0-0.200913-00NiCytoplasmicMembrane1020
PROA_ECOLIP07004proAb0243 JW0233 cytoplasmic peripheral IM protein, Gamma-glutamyl phosphate reductase ProACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN480.168CYT0-0.200913-00NiCytoplasmic10118
SDHB_ECOLIP07014sdhBb0724 JW0714 cytoplasmic peripheral IM protein, Succinate dehydrogenase iron-sulfur subunit SdhBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [6388571] [2987185] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [6388571] [2987185] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane Cytoplasmic (Experimental)yesyesN280.102CYT0-0.200913-00NiCytoplasmicMembrane1034
HISP_ECOLIP07109hisPb2306 JW2303 cytoplasmic peripheral IM protein, Histidine transport ATP-binding HisP Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN310.431CYT0-0.200913-00NiCytoplasmicMembrane1067
CHEA_ECOLIP07363cheAb1888 JW1877 cytoplasmic peripheral IM protein, Chemotaxis CheACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted), Soluble IM Fraction +CytoplasmCytoplasmicnoyesN170.108CYT0-0.200913-00NiCytoplasmic1088
SYFB_ECOLIP07395pheTb1713 JW1703 cytoplasmic peripheral IM protein, Phenylalanine--tRNA ligase beta subunit PheTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN290.131CYT0-0.200913-00NiCytoplasmic979
TYPH_ECOLIP07650deoAtpp ttgb4382 JW4345 cytoplasmic peripheral IM protein, Thymidine phosphorylase DeoACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicnoyesN530.104CYT0-0.200913-00NiCytoplasmic812
FHUC_ECOLIP07821fhuCb0151 JW0147 cytoplasmic peripheral IM protein, Iron(3+)-hydroxamate import ATP-binding FhuCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1551849] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN390.111CYT0-0.200913-00NiCytoplasmicMembrane1045
IDH_ECOLIP08200icdicdA icdEb1136 JW1122 cytoplasmic peripheral IM protein, Isocitrate dehydrogenase (NADP) IcdCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicTFyesyesN470.119CYT0-0.200913-00NiCytoplasmic996
SYFA_ECOLIP08312pheSb1714 JW5277 cytoplasmic peripheral IM protein, Phenylalanine--tRNA ligase alpha subunit PheSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN190.166CYT0-0.200913-00NiCytoplasmic1012
DNAJ_ECOLIP08622dnaJgroPb0015 JW0014 cytoplasmic peripheral IM protein, Chaperone DnaJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN390.105CYT0-0.200913-00NiCytoplasmic1052
PT1_ECOLIP08839ptsIb2416 JW2409 cytoplasmic peripheral IM protein, Phosphoenolpyruvate-phosphotransferase PtsICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[2644649] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN150.115CYT0-0.200913-00NiCytoplasmic1090
NARG_ECOLIP09152narGbisD narCb1224 JW1215 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 1 alpha chain NarGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. [12910261] In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. [19295650] When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. [2674654] The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. [3371362] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12910261] [16286471] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell membrane; Peripheral membrane proteinCytoplasmicyesyesN200.124CYT0-0.200913-00NiCytoplasmicMembrane1029
GRPE_ECOLIP09372grpEb2614 JW2594 cytoplasmic peripheral IM protein, GrpECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN350.101CYT0-0.200913-00NiCytoplasmic9
PFLB_ECOLIP09373pflBpflb0903 JW0886 cytoplasmic peripheral IM protein, Formate acetyltransferase 1 PflBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmicTFyesyesN480.103CYT0-0.200913-00NiCytoplasmic1030
PUTA_ECOLIP09546putApoaAb1014 JW0999 cytoplasmic nucleoid peripheral IM protein, Bifunctional PutACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)The proline dehydrogenase PutA protein from S. typhimurium was shown to act as a membrane-associated enzyme or as a transcriptional repressor [8483946]. Catabolism of proline requires PutA to associate with the membrane because reoxidation of the FAD cofactor in PutA needs the presence of an electron acceptor. Repression of the put operon requires PutA to bind to the put control-region DNA in the cytoplasm. Transcription factor [23138451]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][8483946] [23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCytoplasmicyesyesN640.118CYT0-0.200913-00NiCytoplasmic9
GLTB_ECOLIP09831gltBaspBb3212 JW3179 cytoplasmic peripheral IM protein, Glutamate synthase (NADPH) large chain GltBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN480.135CYT0-0.200913-00NiCytoplasmic9
MODC_ECOLIP09833modCchlD narDb0765 JW0748 cytoplasmic peripheral IM protein, Molybdenum import ATP-binding ModC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmicnoyesN350.128CYT0-0.200913-00NiCytoplasmicMembrane10
UVRA_ECOLIP0A698uvrAdinEb4058 JW4019 cytoplasmic peripheral IM protein, UvrABC system A UvrACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicTFnoyesN420.254CYT0-0.200913-00NiCytoplasmic9
ACKA_ECOLIP0A6A3ackAackb2296 JW2293 cytoplasmic peripheral IM protein, Acetate kinase AckACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN200.328CYT0-0.200913-00NiCytoplasmic10112
ACP_ECOLIP0A6A8acpPb1094 JW1080 cytoplasmic peripheral IM protein, Acyl carrier AcpPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of phospholipid metabolism complex PlsB-PssA-YbgC-Acp[16294310]. Various methods were used to demonstrate that ACP could be cross-linked specifically to an inner membrane protein of E. coli, most probably to the glycerol-3-phosphate acyl transferase (GPAT)[1310877]. Evidence for interactions of acyl carrier protein with glycerol-3-phosphate acyltransferase, an inner membrane protein of Escherichia coli[2676605]. Membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein[2668033]. [3884600] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1310877] [2676605] [2668033] [3884600] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmic (Experimental)pseudogene (Ochman & Davalos, 2006)noyesN470.119CYT0-0.200913-00NiCytoplasmic10
ISCS_ECOLIP0A6B7iscSnuvC yfhO yzzOb2530 JW2514 cytoplasmic peripheral IM protein, Cysteine desulfurase IscSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN480.104CYT0-0.200913-00NiCytoplasmic937
ARGB_ECOLIP0A6C8argBb3959 JW5553 cytoplasmic peripheral IM protein, Acetylglutamate kinase ArgBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN220.115CYT0-0.200913-00NiCytoplasmic981
ASSY_ECOLIP0A6E4argGb3172 JW3140 cytoplasmic peripheral IM protein, Argininosuccinate synthase ArgGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN370.163CYT0-0.200913-00NiCytoplasmic919
ATPE_ECOLIP0A6E6atpCpapG uncCb3731 JW3709 cytoplasmic peripheral IM protein, ATP synthase epsilon chain AtpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[16079137] [21778229] [9868784] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)noyesN410.107CYT0-0.200913-00NiCytoplasmicMembrane10120
CARA_ECOLIP0A6F1carApyrAb0032 JW0030 cytoplasmic peripheral IM protein, Carbamoyl-phosphate synthase small chain CarACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN220.124CYT0-0.200913-00NiCytoplasmic960
GLPK_ECOLIP0A6F3glpKb3926 JW3897 cytoplasmic peripheral IM protein, Glycerol kinase GlpKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN220.143CYT0-0.200913-00NiCytoplasmic921
CH60_ECOLIP0A6F5groLgroEL mopAb4143 JW4103 cytoplasmic peripheral IM protein, 60 kDa chaperonin GroLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN580.12CYT0-0.200913-00NiCytoplasmic1094
CH10_ECOLIP0A6F9groSgroES mopBb4142 JW4102 cytoplasmic peripheral IM protein, 10 kDa chaperonin GroSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [22380631] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN430.185CYT0-0.200913-00NiCytoplasmic1086
HSLU_ECOLIP0A6H5hslUhtpIb3931 JW3902 cytoplasmic peripheral IM protein, ATP-dependent protease ATPase subunit HslUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN240.141CYT0-0.200913-00NiCytoplasmic1074
DADA_ECOLIP0A6J5dadAdadRb1189 JW1178 cytoplasmic peripheral IM protein, D-amino acid dehydrogenase DadACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][6102989] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicTFyesyesN230.155TMH00.264511-00Yn3-11c16/17oCytoplasmicMembrane1012
DEF_ECOLIP0A6K3deffmsb3287 JW3248 cytoplasmic peripheral IM protein, Peptide deformylase DefCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN420.113CYT0-0.200913-00NiCytoplasmic966
DEOB_ECOLIP0A6K6deoBdrm thyRb4383 JW4346 cytoplasmic peripheral IM protein, Phosphopentomutase DeoBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN400.147CYT0-0.200913-00NiCytoplasmic10101
DEOC_ECOLIP0A6L0deoCdra thyRb4381 JW4344 cytoplasmic peripheral IM protein, Deoxyribose-phosphate aldolase DeoCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN220.136CYT0-0.200913-00NiCytoplasmic10115
EFG_ECOLIP0A6M8fusAfar fusb3340 JW3302 cytoplasmic peripheral IM protein, Elongation factor G FusACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation [7042386][23230279] [7042386]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmic (Experimental)yesyesN390.11CYT0-0.200913-00NiCytoplasmic962
EFTS_ECOLIP0A6P1tsfb0170 JW0165 cytoplasmic peripheral IM protein, Elongation factor Ts TsfCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicnoyesN590.109CYT0-0.200913-00NiCytoplasmic1087
DER_ECOLIP0A6P5derengA yfgKb2511 JW5403 ribosomal cytoplasmic peripheral IM protein, GTPase Der Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Essential protein, proposed to be engaged in a functional cyclic interaction between ribosomes and the membrane in Escherichia coli through interaction with the 50S subunit. Assembly cofactor of ribosome; associates with the 50S subunit and YihI. YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20172997] [21354313] [17272300] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasmicyesyesN310.139CYT0-0.200913-00NiCytoplasmic966
ENGB_ECOLIP0A6P7engByihAb3865 JW5930 cytoplasmic peripheral IM protein, Probable GTP-binding EngB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of YqiK have been monitored via fluorescence microscopy. YqiK was found to be distributed diffusely throughout cytoplasm.[17272300] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN430.126CYT0-0.200913-00NiUnknown2
ENO_ECOLIP0A6P9enob2779 JW2750 cytoplasmic peripheral IM protein facing the cytoplasm & peripheral OM protein facing the extra-cellular space (SEC through IM), Enolase EnoCytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Probable)When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. [8610017] [9600841] [9740056] [9732527] [12035760] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17242352] [8610017] [15003462] [18337249] [9600841] [9740056] [9732527] [12035760] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceA, F1, F4Soluble IM FractionCytoplasm; cytoskeleton Secreted Cell surface Cytoplasmic (Experimental)SECyesyesN420.139CYT0-0.200913-00NiCytoplasmic9100
FABZ_ECOLIP0A6Q6fabZsefA yaeAb0180 JW0175 cytoplasmic peripheral IM protein, 3-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. [8354462] [7806516] [8805534] [8910376] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15911532] [8910376] [8354462] [7806516] [8805534] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmic (Experimental)TFyesyesN320.104CYT0-0.200913-00NiCytoplasmic1027
FIS_ECOLIP0A6R3fisb3261 JW3229 cytoplasmic nucleoid peripheral IM protein, DNA-binding FisCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoid-associated protein (NAP).[21541338] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN190.116CYT0-0.200913-00NiCytoplasmic9110
MNMG_ECOLIP0A6U3mnmGgidA trmFb3741 JW3719 cytoplasmic peripheral IM protein, tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Similar localization to mnmE; may form a complex together [17062623]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN250.172CYT0-0.200913-01No601-619iCytoplasmic96
GLGA_ECOLIP0A6U8glgAb3429 JW3392 cytoplasmic peripheral IM protein, Glycogen synthase GlgACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN320.162CYT0-0.200913-00NiCytoplasmic910
HEM1_ECOLIP0A6X1hemAb1210 JW1201 cytoplasmic peripheral IM protein, Glutamyl-tRNA reductase HemACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN440.116CYT0-0.200913-00NiCytoplasmic923
HFQ_ECOLIP0A6X3hfqb4172 JW4130 cytoplasmic nucleoid peripheral IM protein, RNA-binding HfqCytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Nucleoid (Experimental)The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain. Is a bacterial protein involved in several aspects of nucleic acid transactions. Nucleoid-associated protein (NAP) that binds sequence-nonspecific DNA. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][20011543] [21541338] [24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicyesyesN530.107CYT0-0.200913-00NiCytoplasmic1075
IHFA_ECOLIP0A6X7ihfAhid himAb1712 JW1702 cytoplasmic nucleoid peripheral IM protein, Integration host factor subunit alpha IhfACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Translation. Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature [21541338] [23230279] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicnoyesN240.109CYT0-0.200913-00NiCytoplasmic9
IHFB_ECOLIP0A6Y1ihfBhimD hipb0912 JW0895 cytoplasmic nucleoid peripheral IM protein, Integration host factor subunit beta IhfBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Translation, nucleoid-associated protein (NAP). Bacterial histone-like DNA-binding proteins signature [21541338] [23230279] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmCytoplasmicnoyesN390.116CYT0-0.200913-00NiCytoplasmic923
HSLO_ECOLIP0A6Y5hslOyrfIb3401 JW5692 cytoplasmic peripheral IM protein, 33 kDa chaperonin HslOCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (230 232;263 266) [10764757][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmCytoplasmicyesyesN630.125CYT0-0.200913-00NiCytoplasmic1088
DNAK_ECOLIP0A6Y8dnaKgroP grpF segb0014 JW0013 cytoplasmic peripheral IM protein of Hsp70 family, Chaperone DnaKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)DnaK associates with the inner membrane. The ability to isolate DnaK with a membrane fraction prepared under gentle lysis conditions supports a peripheral association between DnaK and the cytoplasmic membrane. [7961473] Overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to green fluorescent protein (GFP) led to an increased association (2.3???4.8-fold) of DnaK with the membrane. [17446557] [1396676] [7783627] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][16079137] [17446557] [1396676] [7783627] [23230279] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)yesyesN200.127CYT0-0.200913-00NiCytoplasmic10115
HTPG_ECOLIP0A6Z3htpGb0473 JW0462 cytoplasmic peripheral IM protein, Chaperone HtpGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN430.189CYT0-0.200913-00NiCytoplasmic10109
IF2_ECOLIP0A705infBgicD ssyGb3168 JW3137 ribosomal cytoplasmic peripheral IM protein, Translation initiation factor IF-2 InfBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Translation. Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasm CytoplasmicTFyesyesN280.114CYT0-0.200913-00NiCytoplasmic965
IF3_ECOLIP0A707infCfit srjAb1718 JW5829 ribosomal cytoplasmic peripheral IM protein, Translation initiation factor IF-3 InfCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Translation. Trigger factor-sensitive aggregation. Evidence that the inter-domain region is disordered in solution.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasm CytoplasmicTFnoyesN220.117CYT0-0.200913-00NiCytoplasmic10113
KPRS_ECOLIP0A717prsprsAb1207 JW1198 cytoplasmic peripheral IM protein, Ribose-phosphate pyrophosphokinase PrsCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN310.131CYT0-0.200913-00NiCytoplasmic109
MINE_ECOLIP0A734minEb1174 JW1163 cytoplasmic peripheral IM protein, Cell division topological specificity factor MinECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature. [21738659][20025670] [10096083] [21738659]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN180.123CYT0-0.200913-00NiCytoplasmic9101
KEFF_ECOLIP0A754kefFyabFb0046 JW0045 cytoplasmic peripheral IM protein, Glutathione-regulated potassium-efflux system ancillary KefF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from <i>Escherichia coli</i>, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity.[17679694]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane CytoplasmicyesyesN170.127CYT0-0.200913-00NiCytoplasmic934
KEFG_ECOLIP0A756kefGyheRb3351 JW3314 cytoplasmic peripheral IM protein, Glutathione-regulated potassium-efflux system ancillary KefG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from Escherichia coli, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17679694] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane CytoplasmicyesyesN230.13CYT0-0.200913-00NiCytoplasmic9
NAGB_ECOLIP0A759nagBglmDb0678 JW0664 cytoplasmic peripheral IM protein, Glucosamine-6-phosphate deaminase NagBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (219 219) [8240271][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN320.107CYT0-0.200913-00NiCytoplasmic828
NDK_ECOLIP0A763ndkb2518 JW2502 cytoplasmic peripheral IM protein, Nucleoside diphosphate kinase NdkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoside diphosphate kinase (EC 2.7.4.6) (Ndk) is a ubiquitous enzyme functioning in the intracellular distribution of terminal phosphate bond energy among the various nucleotides used in synthetic and regulatory functions in cells[8606147]. Based on studies in Pseudomonas and many other systems including mitochondria, the uracil-processing DNA repair nuclease ndk is known to interact to plasma membranes and mitochondrial inner membrane[15096615]. Binding is regulated by nucleotides[19146889]. In Pseudomonas, an intracellular protease cleaves the 16-kDa form of Ndk to a 12-kDa form that undergoes autophosphorylation with an efficiency almost identical to that of the 16-kDa form. The 12-kDa form is predominantly associated with the cell membrane fraction, whereas the 16-kDa form is predominantly cytoplasmic. In the membrane-associated state, the 12-kDa form of Ndk was found to synthesize GTP in preference to other nucleoside triphosphates. The formation of the 12-kDa form of Ndk and its association with the cell membrane were found to be related to the growth stage of <i>P.aeruginosa</i>, with less than 1% of the 12-kDa Ndk detectable in the membrane fraction at early log phase in comparison with the levels present at late stationary phase[8606147]. Cytosolic Ndks bind directly to membrane lipids in a dynamic process and can be fully reconstituted with phospholipids and recombinant Ndk [19146889]. Ndks may act as a reservoir of high energy phosphate, supporting the operation of membrane-based processes that utilize nucleotides other than ATP, such as intracellular traffic and phospholipid biosynthesis. Trigger factor-sensitive aggregation[19737520]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19146889] [8606147] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFnoyesN170.158CYT0-0.200913-00NiCytoplasmic1064
RPPH_ECOLIP0A776rppHnudH ygdPb2830 JW2798 cytoplasmic nucleoid peripheral IM protein, RNA pyrophosphohydrolase RppHCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicyesyesN250.126CYT0-0.200913-00NiCytoplasmic962
ORN_ECOLIP0A784orno204a yjeRb4162 JW5740 cytoplasmic peripheral IM protein, Oligoribonuclease OrnCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN480.121CYT0-0.200913-00NiCytoplasmic9103
PFKA_ECOLIP0A796pfkAb3916 JW3887 cytoplasmic peripheral IM protein, ATP-dependent 6-phosphofructokinase isozyme 1 PfkACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN200.426CYT0-0.200913-00NiCytoplasmic1076
PGK_ECOLIP0A799pgkb2926 JW2893 cytoplasmic peripheral IM protein, Phosphoglycerate kinase PgkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN210.107CYT0-0.200913-00NiCytoplasmic10100
PLSB_ECOLIP0A7A7plsBb4041 JW4001 cytoplasmic peripheral IM protein, Glycerol-3-phosphate acyltransferase PlsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid metabolism complex PlsB-PssA-YbgC-acp Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16294310] [4943977] [15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicnoyesN270.194CYT0-0.200913-00NiCytoplasmicMembrane10
PPK1_ECOLIP0A7B1ppkb2501 JW2486 cytoplasmic peripheral IM protein, Polyphosphate kinase PpkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1331061] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane CytoplasmicyesyesN260.112CYT0-0.200913-00NiCytoplasmicMembrane107
NADK_ECOLIP0A7B3nadKyfjB yfjEb2615 JW2596 cytoplasmic peripheral IM protein, NAD kinase NadKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Fluorescent fusions of ppnK have been monitored via fluorescence microscopy. ppnK distributed irregularly throughout the cytoplasm, with some clusters formed.[17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN200.111CYT0-0.200913-00NiCytoplasmic932
PURA_ECOLIP0A7D4purAadeKb4177 JW4135 cytoplasmic peripheral IM protein, Adenylosuccinate synthetase PurACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN620.102CYT0-0.200913-00NiCytoplasmic1041
PUR7_ECOLIP0A7D7purCb2476 JW2461 cytoplasmic peripheral IM protein, Phosphoribosylaminoimidazole-succinocarboxamide synthase PurCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN650.127CYT0-0.200913-00NiCytoplasmic950
PYRD_ECOLIP0A7E1pyrDb0945 JW0928 cytoplasmic peripheral IM protein, Dihydroorotate dehydrogenase PyrDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell membrane; Peripheral membrane proteinCytoplasmicyesyesN450.146CYT0-0.200913-00NiCytoplasmicMembrane1012
PYRG_ECOLIP0A7E5pyrGb2780 JW2751 cytoplasmic peripheral IM protein, CTP synthase PyrGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)PyrG=CTP synthase or CtpS in some systems. CTP synthase forms filaments in Caulobacter crescentus that regulate cell curvature independently of its catalytic function. The morphogenic role of CtpS requires its functional interaction with the intermediate filament, crescentin (CreS). Trigger factor-sensitive aggregation. [19737520] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20639870] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicTFyesyesN270.24SpI261.52462-10NiCytoplasmic977
PYRH_ECOLIP0A7E9pyrHsmbAb0171 JW0166 cytoplasmic peripheral IM protein, Uridylate kinase PyrHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of PyrH have been monitored via fluorescence microscopy. PyrH was found to be located in one membrane-associated foci per cell. [17272300] PyrH-EYFP fusions are predominantly localized near the bacterial membranes of different cells undergoing septation and division. [9922246] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9922246] [17272300] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN230.14CYT0-0.200913-00NiCytoplasmic930
RBFA_ECOLIP0A7G2rbfAP15B yhbBb3167 JW3136 ribosomal cytoplasmic peripheral IM protein, 30S ribosome-binding factor RbfACytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Assembly cofactor of ribosome; associate with the 30S subunit. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN510.152CYT0-0.200913-00NiCytoplasmic1068
RECA_ECOLIP0A7G6recAlexB recH rnmB tif umuB zabb2699 JW2669 cytoplasmic peripheral IM protein, RecACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544][2228969]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmicTFnoyesN30.103CYT0-0.200913-00NiCytoplasmic1088
RECF_ECOLIP0A7H0recFuvrFb3700 JW3677 cytoplasmic nucleoid peripheral IM protein, DNA replication and repair RecFCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. The prokaryotic protein recF is a single-stranded DNA-binding protein which also probably binds ATP. RecF is involved in DNA metabolism; it is required for recombinational DNA repair and for induction of the SOS response. [1542576][19766000] [1542576]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN320.128CYT0-0.200913-00NiCytoplasmic96
RIBB_ECOLIP0A7J0ribBhtrPb3041 JW3009 cytoplasmic peripheral IM protein, 3,4-dihydroxy-2-butanone 4-phosphate synthase RibBCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane CytoplasmicyesyesN220.191CYT0-0.200913-00NiCytoplasmic9116
RIMM_ECOLIP0A7X6rimMyfjAb2608 JW5413 ribosomal cytoplasmic peripheral IM protein, Ribosome maturation factor RimM Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Assembly cofactor of ribosome; associate with the 30S subunit. [11514519] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11514519] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasm CytoplasmicyesyesN390.124CYT0-0.200913-00NiCytoplasmic9106
RNC_ECOLIP0A7Y0rncb2567 JW2551 cytoplasmic nucleoid peripheral IM protein, Ribonuclease 3 RncCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][4865702] [24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicTFyesyesN560.133CYT0-0.200913-00NiCytoplasmic10116
RPOA_ECOLIP0A7Z4rpoApez phs sezb3295 JW3257 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit alpha RpoACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338][23230279] [21541338]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicyesyesN250.163CYT0-0.200913-00NiCytoplasmic9
RPOZ_ECOLIP0A800rpoZb3649 JW3624 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit omega RpoZCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)The presence of omega subunit of RNA polymerase (RpoZ) is necessary for maturation of RNA polymerase. Experimentally verified gene-product. Methionine start codon is cleaved.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicyesyesN350.113CYT0-0.200913-00NiCytoplasmic881
RRF_ECOLIP0A805frrrrfb0172 JW0167 cytoplasmic peripheral IM protein, Ribosome-recycling factor FrrCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN330.103CYT0-0.200913-00NiCytoplasmic10109
METK_ECOLIP0A817metKmetXb2942 JW2909 cytoplasmic peripheral IM protein, S-adenosylmethionine synthase MetKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of MetK have been monitored via fluorescence microscopy. Cells containing almost exclusively diffuse cytoplasmic MetK, or focused locations.[17272300] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN640.118CYT0-0.200913-00NiCytoplasmic1047
GLYA_ECOLIP0A825glyAb2551 JW2535 cytoplasmic peripheral IM protein, Serine hydroxymethyltransferase Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN470.123CYT0-0.200913-00NiCytoplasmic10
SUCC_ECOLIP0A836sucCb0728 JW0717 cytoplasmic peripheral IM protein, Succinate--CoA ligase SucCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN320.181CYT0-0.200913-00NiCytoplasmic867
TIG_ECOLIP0A850tigb0436 JW0426 cytoplasmic peripheral IM protein, Trigger factor TigCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit [8633085] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [8633085] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN210.128CYT0-0.200913-00NiCytoplasmic887
TPIS_ECOLIP0A858tpiAtpib3919 JW3890 cytoplasmic peripheral IM protein, Triosephosphate isomerase TpiACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN400.118CYT0-0.200913-00NiCytoplasmic996
TPX_ECOLIP0A862tpxyzzJb1324 JW1317 cytoplasmic peripheral IM protein, Thiol peroxidase TpxCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. [7499381] [9298646] Disulfide bond-containing protein [25664544][23230279] [7499381] [9298646]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/APeriplasm Periplasmic (Experimental)yesyesN330.123CYT0-0.200913-00NiPeriplasmic10
TALB_ECOLIP0A870talByaaKb0008 JW0007 cytoplasmic peripheral IM protein, Transaldolase B TalBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN330.122CYT0-0.200913-00NiCytoplasmic884
RAPZ_ECOLIP0A894rapZyhbJb3205 JW3172 cytoplasmic peripheral IM protein, RNase adapter RapZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN180.112CYT0-0.200913-00NiUnknown738
RIMP_ECOLIP0A8A8rimPyhbCb3170 JW5533 cytoplasmic peripheral IM protein, Ribosome maturation factor RimP Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm ( Experimental)Assembly cofactor of ribosome; associate with the 30S subunit.[21529161]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN190.107CYT0-0.200913-00NiCytoplasmic9111
YIHI_ECOLIP0A8H6yihIb3866 JW3837 cytoplasmic peripheral IM protein, Der GTPase-activating YihICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associates with Der.[21529161]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN430.101CYT0-0.200913-00NiUnknown2108
RHLB_ECOLIP0A8J8rhlBmmrAb3780 JW3753 cytoplasmic nucleoid peripheral IM protein, ATP-dependent RNA helicase RhlBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [18337249] [24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicyesyesN560.132CYT0-0.200913-00NiCytoplasmic9
PSD_ECOLIP0A8K1psdb4160 JW4121 cytoplasmic peripheral IM protein, Phosphatidylserine decarboxylase proenzyme PsdCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Phosphatidylserine decarboxylase is composed of two nonidentical subunits, alpha and beta, derived from a single proenzyme. The proenzyme is processed in a post-translational event into the heterodimer. This enzyme is unique among the known pyruvoyl-dependent decarboxylases because it is membrane-associated. [3042771] The phosphatidylserine decarboxylase (EC 4.1.1.65, phosphatidyl-L-serine carboxy-lyase) of Escherichia coli is associated with the cytoplasmic membrane and requires nonionic detergent for solubilization and detection of enzymatic activity [1495415] A strain of Escherichia coli bearing a hybrid plasmid containing the psd gene, starved for isoleucine by the addition of valine, produces amounts of phosphatidyl-serine decarboxylase, a membrane-bound enzyme, about 40-fold higher than wild type. [368058] The first phospholipid biosynthetic enzyme to be purified to homogeneity was Psd of E. coli. Beginning in the late 1960s purification of membrane proteins after solubilization with detergents was an increasingly popular endeavor. [22925633] [3042771] [5543671] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3042771] [1495415] [368058] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell membrane Cytoplasmic (Experimental)noyesN230.185CYT0-0.200913-00NiCytoplasmicMembrane1032
SYS_ECOLIP0A8L1serSb0893 JW0876 cytoplasmic peripheral IM protein, Serine--tRNA ligase SerSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN670.1CYT0-0.200913-00NiCytoplasmic1028
SYN_ECOLIP0A8M0asnStssb0930 JW0913 cytoplasmic peripheral IM protein, Asparagine--tRNA ligase AsnSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit. Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicTFyesyesN530.182CYT0-0.200913-00NiCytoplasmic1070
SYT_ECOLIP0A8M3thrSb1719 JW1709 cytoplasmic peripheral IM protein, Threonine--tRNA ligase ThrSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN330.154CYT0-0.200913-00NiCytoplasmic1022
MATP_ECOLIP0A8N0matPycbGb0956 JW0939 cytoplasmic nucleoid peripheral IM protein, Macrodomain Ter protein MatPCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)[18984159]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmyesyesN370.125CYT0-0.200913-00NiCytoplasmic892
FETP_ECOLIP0A8P3yggXb2962 JW2929 cytoplasmic peripheral IM protein, Probable Fe(2+)-trafficking protein YggXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally verified gene-product. Methionine start codon is cleaved. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN410.14CYT0-0.200913-00Yn4-11c16/17oUnknown2107
RRAA_ECOLIP0A8R0rraAmenG yiiVb3929 JW3900 cytoplasmic nucleoid peripheral IM protein, Regulator of ribonuclease activity A RraACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Negative regulator of RNaseE. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicnoyesN340.108CYT0-0.200913-00NiCytoplasmic9
RPOC_ECOLIP0A8T7rpoCtabBb3988 JW3951 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit beta' RpoCCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicTFyesyesN260.118CYT0-0.200913-00NiCytoplasmic9
SYDP_ECOLIP0A8U0sydydrb2793 JW2764 cytoplasmic peripheral IM protein, SydCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane protein; Cytoplasmic side CytoplasmicyesyesN200.125CYT0-0.200913-00NiCytoplasmicMembrane1059
RPOB_ECOLIP0A8V2rpoBgroN nitB rif ron stl stv tabDb3987 JW3950 cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit beta RpoBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicTFyesyesN400.117CYT0-0.200913-00NiCytoplasmic9
YJGA_ECOLIP0A8X0yjgAb4234 JW4193 cytoplasmic peripheral IM protein, UPF0307 YjgACytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal (Experimental)[23230279] [16980477]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasm CytoplasmicyesyesN460.101CYT0-0.200913-00NiCytoplasmic981
YBGC_ECOLIP0A8Z3ybgCb0736 JW0726 cytoplasmic peripheral IM protein, Acyl-CoA thioester hydrolase YbgCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Phospholipid metabolism complex PlsB-PssA-YbgC-acp [16294310]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN520.116CYT0-0.200913-00NiCytoplasmic970
RIMJ_ECOLIP0A948rimJb1066 JW1053 cytoplasmic peripheral IM protein, Ribosomal-protein-alanine acetyltransferase RimJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associate with the Pre-30S subunit. Overex<x>pression of RimJ suppresses defects in S5(G28D) mutations.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN330.107CYT0-0.200913-00NiCytoplasmic10
FABB_ECOLIP0A953fabBfabCb2323 JW2320 cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) synthase 1 FabBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN250.163CYT0-0.200913-00Yn4-15c24/25oCytoplasmic1013
ALKH_ECOLIP0A955edahga kdgAb1850 JW1839 cytoplasmic peripheral IM protein, KHG/KDPG aldolase EdaCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN300.126CYT0-0.200913-00NiCytoplasmic1077
ALF1_ECOLIP0A991fbaBdhnAb2097 JW5344 cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 1 FbaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN640.115CYT0-0.200913-00NiUnknown24
GLPC_ECOLIP0A996glpCb2243 JW2237 cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit C GlpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. [3286606] [3286606]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane protein Membrane associated (Experimental)yesyesN350.222CYT0-0.200913-00NiCytoplasmicMembrane1013
FTNB_ECOLIP0A9A2ftnByecIb1902 JW1890 cytoplasmic peripheral IM protein, Bacterial non-heme ferritin-like protein FtnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN480.152CYT0-0.200913-00NiCytoplasmic1082
FTSZ_ECOLIP0A9A6ftsZsfiB sulBb0095 JW0093 cytoplasmic peripheral IM protein, Cell division FtsZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. Assembles at midcell at the inner surface of the cytoplasmic membrane. SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. [8917533] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Over-expression of NlpI can interrupt the nucleoids division and the assembly of FtsZ at the septum, mediated by IbpA/IbpB, suggesting a role of the NlpI/IbpA/IbpB complex in the cell division. [25699035][1944597] [20497333] [8917533] [23230279] [25699035]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN260.12CYT0-0.200913-00NiCytoplasmicMembrane1079
G3P1_ECOLIP0A9B2gapAb1779 JW1768 cytoplasmic peripheral IM protein, Glyceraldehyde-3-phosphate dehydrogenase A GapACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicTFyesyesN210.121CYT0-0.200913-00NiCytoplasmic973
GLPA_ECOLIP0A9C0glpAb2241 JW2235 cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit A GlpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane protein CytoplasmicyesyesN620.155CYT0-0.200913-00NiCytoplasmicMembrane105
DAPD_ECOLIP0A9D8dapDb0166 JW0161 cytoplasmic peripheral IM protein, 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase DapDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN240.195CYT0-0.200913-00NiCytoplasmic1041
CYSB_ECOLIP0A9F3cysBb1275 JW1267 cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator CysBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1IM (predicted)CytoplasmCytoplasmicyesyesN240.141CYT0-0.200913-00NiCytoplasmic108
ACEA_ECOLIP0A9G6aceAiclb4015 JW3975 cytoplasmic peripheral IM protein, Isocitrate lyase AceACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN300.126CYT0-0.200913-00NiCytoplasmic1042
CFA_ECOLIP0A9H7cfacdfAb1661 JW1653 cytoplasmic peripheral IM protein, Cyclopropane-fatty-acyl-phospholipid synthase CfaCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN380.183CYT0-0.200913-00NiCytoplasmic1026
CHEZ_ECOLIP0A9H9cheZb1881 JW1870 cytoplasmic peripheral IM protein, phosphatase CheZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [12644507][19766000] [12644507]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN660.129CYT0-0.200913-00NiCytoplasmic10107
PHOL_ECOLIP0A9K3ybeZb0660 JW0657 cytoplasmic peripheral IM protein, PhoH-like protein YbeZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN680.101CYT0-0.200913-00NiCytoplasmic961
PHOU_ECOLIP0A9K7phoUnmpAb3724 JW3702 cytoplasmic peripheral IM protein, Phosphate-specific transport system accessory PhoU Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3536855] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN170.113CYT0-0.200913-00NiCytoplasmic1094
SLYD_ECOLIP0A9K9slyDb3349 JW3311 cytoplasmic peripheral IM protein, FKBP-type peptidyl-prolyl cis-trans isomerase SlyD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN310.112CYT0-0.200913-00NiCytoplasmic10
XGPT_ECOLIP0A9M5gptgpp gxub0238 JW0228 cytoplasmic peripheral IM protein, Xanthine phosphoribosyltransferase GptCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN480.21CYT0-0.200913-00NiCytoplasmic10
DLDH_ECOLIP0A9P0lpdAlpdb0116 JW0112 cytoplasmic peripheral IM protein, Dihydroyl dehydrogenase LpdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. [6352260] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bridge (45 50) Disulfide bond-containing protein [25664544][16079137] [6352260] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)TFyesyesN230.176CYT0-0.200913-00NiCytoplasmic1079
DEAD_ECOLIP0A9P6deaDcsdA mssB rhlDb3162 JW5531 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase DeaDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation [19737520]. Contains a SPOR domain. [8552679][19880599] [23230279] [8552679]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN350.151CYT0-0.200913-00NiCytoplasmic1050
ARCA_ECOLIP0A9Q1arcAcpxC dye fexA msp seg sfrAb4401 JW4364 cytoplasmic nucleoid peripheral IM protein, Aerobic respiration control ArcACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[18792681] [23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicyesyesN280.105CYT0-0.200913-00NiCytoplasmic1090
ACCD_ECOLIP0A9Q5accDdedB usgb2316 JW2313 cytoplasmic peripheral IM protein, Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta AccDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation related.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN180.112CYT0-0.200913-00NiCytoplasmic962
ADHE_ECOLIP0A9Q7adhEanab1241 JW1228 cytoplasmic peripheral IM protein, Aldehyde-alcohol dehydrogenase AdhECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN560.127CYT0-0.200913-00NiCytoplasmic945
DHAS_ECOLIP0A9Q9asdhomb3433 JW3396 cytoplasmic peripheral IM protein, Aspartate-semialdehyde dehydrogenase AsdCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN440.197CYT0-0.200913-00NiCytoplasmic918
FTSE_ECOLIP0A9R7ftsEb3463 JW3428 cytoplasmic peripheral IM protein, Cell division ATP-binding FtsECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Is the putative ATP-binding protein component of an ATP-binding cassette (ABC) transporter. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. [10048040] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10048040] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN320.118CYT0-0.200913-00NiCytoplasmicMembrane1011
GATD_ECOLIP0A9S3gatDb2091 JW2075 cytoplasmic peripheral IM protein, Galactitol 1-phosphate 5-dehydrogenase GatDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicTFnoyesN530.115CYT0-0.200913-00NiCytoplasmic953
LIVG_ECOLIP0A9S7livGb3455 JW3420 cytoplasmic peripheral IM protein, High-affinity branched-chain amino acid transport ATP-binding LivG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN210.325CYT0-0.200913-00NiCytoplasmic911
YBBA_ECOLIP0A9T8ybbAb0495 JW0484 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YbbACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalKCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN410.105CYT0-0.200913-00NiCytoplasmicMembrane10102
YBHF_ECOLIP0A9U1ybhFb0794 JW5104 cytoplasmic peripheral IM protein, Probable multidrug ABC transporter ATP-binding YbhF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN380.115CYT0-0.200913-00NiCytoplasmicMembrane742
YBIT_ECOLIP0A9U3ybiTb0820 JW0804 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YbiTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN190.108CYT0-0.200913-00NiCytoplasmic942
LPTB_ECOLIP0A9V1lptByhbGb3201 JW3168 cytoplasmic peripheral IM protein, polysaccharide export system ATP-binding LptB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)[17056748] [16079137] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic(experimental)yesyesN430.117CYT0-0.200913-00NiCytoplasmicMembrane1019
ETTA_ECOLIP0A9W3ettAyjjKb4391 JW4354 cytoplasmic peripheral IM protein, Energy-dependent translational throttle EttACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN430.116CYT0-0.200913-00NiCytoplasmic992
ZNUC_ECOLIP0A9X1znuCyebMb1858 JW1847 cytoplasmic peripheral IM protein, Zinc import ATP-binding ZnuC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN220.118CYT0-0.200913-00NiCytoplasmicMembrane715
MREB_ECOLIP0A9X4mreBenvB rodYb3251 JW3220 cytoplasmic peripheral IM protein, Cell shape-determining MreBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Bacterial actin homologue; is required for cell shape maintenance in most non-spherical bacteria, where it assembles into helical structures just underneath the cytoplasmic membrane; binds to the bitopic inner membrane protein RodZ as monomer or multimer. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [19737520] PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones. [PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones. [24057063] [17090951] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20168300] [19078962] [21816350] [19008860] [16391237] [24057063] [17090951] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)TFyesyesN410.127CYT0-0.200913-00NiCytoplasmic962
PTHP_ECOLIP0AA04ptsHhprb2415 JW2408 cytoplasmic peripheral IM protein, Phosphocarrier HPr PtsHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicnoyesN220.11CYT0-0.200913-00NiCytoplasmic1088
OMPR_ECOLIP0AA16ompRkmt ompBb3405 JW3368 cytoplasmic nucleoid peripheral IM protein, transcriptional regulator OmpRCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN380.124CYT0-0.200913-00NiCytoplasmic1087
THIO_ECOLIP0AA25trxAfipA tsnCb3781 JW5856 cytoplasmic peripheral IM protein, Thioredoxin 1 TrxACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Disulfide bridge (33 36) [10489448] Disulfide bond-containing protein [25664544][23230279] [10489448]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFnoyesN200.119CYT0-0.200913-00NiCytoplasmic989
CLSB_ECOLIP0AA84clsBybhOb0789 JW0772 cytoplasmic peripheral IM protein, Cardiolipin synthase B ClsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. [22988102][22988102]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane CytoplasmicyesyesN660.136CYT0-0.200913-00NiCytoplasmicMembrane727
UBID_ECOLIP0AAB4ubiDyigC yigYb3843 JW3819 cytoplasmic peripheral IM protein, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell membrane CytoplasmicyesyesN650.107CYT0-0.200913-00NiCytoplasmic937
USPD_ECOLIP0AAB8uspDyiiTb3923 JW3894 cytoplasmic peripheral IM protein, Universal stress D UspDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN540.108CYT0-0.200913-00NiCytoplasmic996
ARAG_ECOLIP0AAF3araGb1900 JW1888 cytoplasmic peripheral IM protein, Arabinose import ATP-binding AraGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)[7028715]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN380.293CYT0-0.200913-00NiCytoplasmicMembrane1037
ARTP_ECOLIP0AAF6artPb0864 JW0848 cytoplasmic peripheral IM protein, Arginine transport ATP-binding ArtPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) The artPIQM artJ genes encode a third arginine-uptake system in addition to the known argT hisJQMP system of Salmonella typhimurium and E. coli and the arginine (-ornithine) carrier (aps) of E. coli. The artQ, artM and artP products are similar to the transmembraneous proteins and the ATPase of binding-protein-dependent carriers. [8801422] [8801422] [15758229] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][8801422] [15758229] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)yesyesN200.124CYT0-0.200913-00NiCytoplasmicMembrane726
DPPD_ECOLIP0AAG0dppDb3541 JW3510 cytoplasmic peripheral IM protein, Dipeptide transport ATP-binding DppDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN660.286CYT0-0.200913-00NiCytoplasmicMembrane719
GLTL_ECOLIP0AAG3gltLb0652 JW0647 cytoplasmic peripheral IM protein, Glutamate/aspartate import ATP-binding GltLCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN270.108CYT0-0.200913-00NiCytoplasmicMembrane943
MGLA_ECOLIP0AAG8mglAb2149 JW2136 cytoplasmic peripheral IM protein, Galactose/methyl galactoside import ATP-binding MglA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[15919657]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN290.181CYT0-0.200913-00NiCytoplasmicMembrane1014
PSTB_ECOLIP0AAH0pstBphoTb3725 JW3703 cytoplasmic peripheral IM protein, Phosphate import ATP-binding PstB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3881386] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN410.133CYT0-0.200913-00NiCytoplasmicMembrane1023
SAPD_ECOLIP0AAH4sapDtrkEb1291 JW1284 cytoplasmic peripheral IM protein, Putrescine export system ATP-binding SapD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN530.145CYT0-0.200913-00NiCytoplasmicMembrane722
SAPF_ECOLIP0AAH8sapFb1290 JW1283 cytoplasmic peripheral IM protein, Putrescine export system ATP-binding SapF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN450.122CYT0-0.200913-00NiCytoplasmicMembrane78
SSUB_ECOLIP0AAI1ssuBycbEb0933 JW0916 cytoplasmic peripheral IM protein, Aliphatic sulfonates import ATP-binding SsuBCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN220.181CYT0-0.200913-00NiCytoplasmicMembrane1023
FABF_ECOLIP0AAI5fabFfabJb1095 JW1081 cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) synthase 2 FabFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN480.158CYT0-0.200913-00Yn6-14c20/21oCytoplasmic98
FDOH_ECOLIP0AAJ5fdoHb3893 JW3864 cytoplasmic peripheral IM protein, Formate dehydrogenase-O iron-sulfur subunit FdoHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of FDH-O isoenzyme complex whose physiological role is to ensure rapid adaptation during a shift from aerobiosis to anaerobiosis. FDH-O is a membrane-bound enzyme complex composed of three subunits, alpha (FdoG), beta (FdoH), and gamma (FdoI). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21890895] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell membrane; Single-pass membrane proteinMembrane anchoredyesyesN240.119CYT0-0.200913-00NiCytoplasmicMembrane1077
YDHY_ECOLIP0AAL6ydhYb1674 JW1664 cytoplasmic peripheral IM protein, Ferredoxin-like YdhYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN280.128CYT0-0.200913-00NiCytoplasmic931
ALF_ECOLIP0AB71fbaAfba fdab2925 JW2892 cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 2 FbaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. [2649077][23230279] [2649077]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmic (Experimental)TFyesyesN460.11CYT0-0.200913-00NiCytoplasmic969
KBL_ECOLIP0AB77kblb3617 JW3592 cytoplasmic peripheral IM protein, 2-amino-3-ketobutyrate coenzyme A ligase KblCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN340.12CYT0-0.200913-00NiCytoplasmic937
PUR8_ECOLIP0AB89purBb1131 JW1117 cytoplasmic peripheral IM protein, Adenylosuccinate lyase PurBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN610.215CYT0-0.200913-00NiCytoplasmic974
ATPD_ECOLIP0ABA4atpHpapE uncHb3735 JW3713 cytoplasmic peripheral IM protein, ATP synthase subunit delta AtpHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicnoyesN360.135CYT0-0.200913-00NiCytoplasmic1089
ATPG_ECOLIP0ABA6atpGpapC uncGb3733 JW3711 cytoplasmic peripheral IM protein, ATP synthase gamma chain AtpGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [7508444] [2900236] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [7508444] [2900236] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)yesyesN410.162CYT0-0.200913-00NiCytoplasmicMembrane1048
ATPA_ECOLIP0ABB0atpApapA uncAb3734 JW3712 cytoplasmic peripheral IM protein, ATP synthase subunit alpha AtpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane CytoplasmicTFnoyesN280.106CYT0-0.200913-00NiCytoplasmic1060
ATPB_ECOLIP0ABB4atpDpapB uncDb3732 JW3710 cytoplasmic peripheral IM protein, ATP synthase subunit beta AtpDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN230.123CYT0-0.200913-00NiCytoplasmicMembrane1077
ACCA_ECOLIP0ABD5accAb0185 JW0180 cytoplasmic peripheral IM protein, Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha AccACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN270.103CYT0-0.200913-00NiCytoplasmic1028
PCNB_ECOLIP0ABF1pcnBb0143 JW5808 cytoplasmic peripheral IM protein, PcnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)RNA degradosome component/adds polyA tails to mRNAs targeted for degradation. The pcnB gene product, is the main enzyme responsible for RNA polyadenylation in E. coli. Polyadenylated RNA molecules are rapidly degraded by a multiprotein complex called RNA degradosome. PAP I localizes in the cytosol but also, like the RNA degradosome, in cellular membranes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15737627] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cytoplasm CytoplasmicyesyesN260.111CYT0-0.200913-00NiCytoplasmic923
FTSA_ECOLIP0ABH0ftsAdivAb0094 JW0092 cytoplasmic peripheral IM protein, Cell division FtsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)FtsA has a conserved C-terminal membrane targeting sequence (MTS) with extremely weak TmHMM and Phobius predictions. Deletion or point mutations in the MTS, inactivate FtsA function and inhibit cell division. Because FtsA binds to the tubulin-like FtsZ protein that forms the Z ring, it is thought that the MTS of FtsA is required, along with the transmembrane protein ZipA, to assemble the Z ring and anchor it to the cytoplasmic membrane. FtsA function in cell division depends on additive effects of membrane binding and self-interaction, and that the specific requirement of an amphipathic helix for tethering FtsA to the membrane can be bypassed. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18186792] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN250.12CYT0-0.200913-00NiCytoplasmic984
CISY_ECOLIP0ABH7gltAgluT icdBb0720 JW0710 cytoplasmic peripheral IM protein, Citrate synthase GltACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN430.113CYT0-0.200913-00NiCytoplasmic922
CYSK_ECOLIP0ABK5cysKcysZb2414 JW2407 cytoplasmic peripheral IM protein, Cysteine synthase A CysKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN310.116CYT0-0.200913-00NiCytoplasmic977
DEOD_ECOLIP0ABP8deoDpupb4384 JW4347 cytoplasmic peripheral IM protein, Purine nucleoside phosphorylase DeoD-type DeoDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN270.108CYT0-0.200913-00NiCytoplasmic9108
DPS_ECOLIP0ABT2dpspexB vtmb0812 JW0797 cytoplasmic nucleoid peripheral IM protein, DNA protection during starvation DpsCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Nucleoid-associated protein (NAP) Dodecameric Dps forms a protein cage with a hollow interior that sequesters Fe 2+ and reduces hydroxyl radical formation [23701194] [7984106][21541338] [23230279] [7984106]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCytoplasm; nucleoidCytoplasmic (Experimental)yesyesN530.125CYT0-0.200913-00NiCytoplasmic1044
FLIG_ECOLIP0ABZ1fliGfla AII.2 fla BIIb1939 JW1923 Flagellar cytoplasmic peripheral IM protein, motor switch FliG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Flaggelar C-ring proteins - FliG, FliM, and FliN. MotA and MotB are ion-conducting membrane proteins that form the stator of the flagellum motor. FliG is a component of the rotor, present in about 25 copies per flagellum. The C-terminal domain of FliG is a component of the rotor in the bacterial flagellar motor. [10440379] [17085573] [16077109] [8631704][10440379] [17085573] [16077109] [8631704]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane protein; Cytoplasmic side Bacterial flagellum basal bodyMembrane associated (Experimental)flagellumnoyesN230.251CYT0-0.200913-00NiCytoplasmic980
KDSC_ECOLIP0ABZ4kdsCyrbI yrbJb3198 JW3165 cytoplasmic peripheral IM protein, 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Short signal peptide of 17aa. Prediction by Phobius and the SignalP NN approach/extensive islands of hydrophobicity by K/D. No obvious biochemical reason that the enzyme should be secreted. Signal peptide like sequence could act as peripheral membrane association element. Lipopolysaccharide (LPS) is an amphiphilic molecule composed of a hydrophilic heteropolysaccharide and the membrane-embedded Lipid A. The innermost 8-carbon sugar 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) is the only conserved structural element found in all LPS, linking the lipid A to the carbohydrate domain of the molecule. The KDO 8-phosphate phosphatase (KdsC) is one of the enzymes of the KDO biosynthesis pathway and is responsible for cleavage of the phosphate group from KDO 8-phosphate to yield KDO. [12639950] In Escherichia coli, KdsC has been characterized as a homotetrameric molecule with a random-coiled C-terminal tail in each subunit. [12639950] [12639950][12639950]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmic (Experimental)yesyesN180.326CYT0-0.200913-00Yn4-12c17/18oCytoplasmic990
FUMA_ECOLIP0AC33fumAb1612 JW1604 cytoplasmic peripheral IM protein, Fumarate hydratase class I, aerobic FumACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN570.116CYT0-0.200913-00NiCytoplasmic937
ASPA_ECOLIP0AC38aspAb4139 JW4099 cytoplasmic peripheral IM protein, Aspartate ammonia-lyase AspACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected with chromosomally tap-tagged TF with >80% confidence score [15690043][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN220.123CYT0-0.200913-00NiCytoplasmic987
SDHA_ECOLIP0AC41sdhAb0723 JW0713 cytoplasmic peripheral IM protein, Succinate dehydrogenase flavosubunit SdhACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [9600841] [9740056] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [12560550] [9600841] [9740056] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)TFyesyesN500.189CYT0-0.200913-00NiCytoplasmicMembrane1010
FRDB_ECOLIP0AC47frdBb4153 JW4114 cytoplasmic peripheral IM protein, Fumarate reductase iron-sulfur subunit FrdBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)FrdA and FrdB fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). [PMC323229] [8419359] [6751816] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][PMC323229] [8419359] [6751816] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)noyesN280.105CYT0-0.200913-00NiCytoplasmicMembrane991
GLNK_ECOLIP0AC55glnKybaIb0450 JW0440 cytoplasmic peripheral IM protein, Nitrogen regulatory P-II 2 GlnKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Encodes a member of the PII signal transduction protein family, proteins that regulate enzyme activity and gene expression in response to the intracellular nitrogen status; cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the the ammonium transporter AmtB.[11847102] [9720863] [10231487] [14668330]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)yesyesN670.123CYT0-0.200913-00NiCytoplasmicMembrane741
GLRX3_ECOLIP0AC62grxCyibMb3610 JW3585 cytoplasmic peripheral IM protein, Glutaredoxin 3 GrxCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (12 15) [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN300.112CYT0-0.200913-00NiUnknown279
GLRX4_ECOLIP0AC69grxDydhDb1654 JW1646 cytoplasmic peripheral IM protein, Glutaredoxin 4 GrxDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [15833738]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN420.264CYT0-0.200913-00NiUnknown290
DNAB_ECOLIP0ACB0dnaBgroP grpAb4052 JW4012 cytoplasmic nucleoid peripheral IM protein, Replicative DNA helicase DnaBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicTFyesyesN30.105CYT0-0.200913-00NiCytoplasmic981
HEMG_ECOLIP0ACB4hemGyihBb3850 JW3827 cytoplasmic peripheral IM protein, Protoporphyrinogen IX dehydrogenase HemGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN330.11CYT0-0.200913-00NiUnknown275
ERPA_ECOLIP0ACC3erpAyadRb0156 JW0152 cytoplasmic peripheral IM protein, Iron-sulfur cluster insertion ErpA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN490.128CYT0-0.200913-00NiCytoplasmic984
GLMU_ECOLIP0ACC7glmUyieAb3730 JW3708 cytoplasmic peripheral IM protein, Bifunctional GlmUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN490.123CYT0-0.200913-00NiCytoplasmic1094
ISCU_ECOLIP0ACD4iscUnifU yfhNb2529 JW2513 cytoplasmic peripheral IM protein, Iron-sulfur cluster assembly scaffold IscU Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN200.105CYT0-0.200913-00NiCytoplasmic997
MBHL_ECOLIP0ACD8hyaBb0973 JW0955 cytoplasmic peripheral IM protein, Hydrogenase-1 large chain HyaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Peripherar Inner Membrane Cyto side (Potential) Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. [22769583] [2180913][22769583] [2180913]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane; Peripheral membrane proteinPeriplasmic (Experimental)yesyesN490.102CYT0-0.200913-00NiCytoplasmicMembrane109
HINT_ECOLIP0ACE7hinTycfFb1103 JW1089 cytoplasmic peripheral IM protein, Purine nucleoside phosphoramidase HinTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN570.182CYT0-0.200913-00NiCytoplasmic984
DBHA_ECOLIP0ACF0hupAb4000 JW3964 cytoplasmic nucleoid peripheral IM protein, DNA-binding HU-alpha HupACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature (<a href="http://prosite.expasy.org/PDOC00044" targer="_blank">PDOC00044</a>).[21541338] [23230279] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN240.244CYT0-0.200913-00NiCytoplasmic996
DBHB_ECOLIP0ACF4hupBhopDb0440 JW0430 cytoplasmic nucleoid peripheral IM protein, DNA-binding HU-beta HupBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature [21541338] [23230279] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicyesyesN270.114CYT0-0.200913-00NiCytoplasmic9
HNS_ECOLIP0ACF8hnsbglY cur drdX hnsA msyA osmZ pilG topSb1237 JW1225 cytoplasmic nucleoid peripheral IM protein, DNA-binding H-NS Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338].[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN190.173CYT0-0.200913-00NiCytoplasmic10103
STPA_ECOLIP0ACG1stpAhnsBb2669 JW2644 cytoplasmic nucleoid peripheral IM protein, DNA-binding StpACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338].[21541338] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN190.184CYT0-0.200913-00NiCytoplasmic915
LRP_ECOLIP0ACJ0lrpalsB ihb livR oppIb0889 JW0872 cytoplasmic nucleoid peripheral IM protein, Leucine-responsive regulatory protein LrpCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1IM (predicted)CytoplasmicyesyesN480.101CYT0-0.200913-00NiCytoplasmic936
CRP_ECOLIP0ACJ8crpcap csmb3357 JW5702 cytoplasmic nucleoid peripheral IM protein, cAMP-activated global transcriptional regulator CrpCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation.[15650111] [23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicTFyesyesN240.105CYT0-0.200913-00NiCytoplasmic9
YFIA_ECOLIP0AD49raiAyfiAb2597 JW2578 cytoplasmic peripheral IM protein, Ribosome-associated inhibitor A RaiACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN630.108CYT0-0.200913-00NiCytoplasmic1098
KPYK1_ECOLIP0AD61pykFb1676 JW1666 cytoplasmic peripheral IM protein, Pyruvate kinase I PykFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN420.167CYT0-0.200913-00NiCytoplasmic9108
AMPH_ECOLIP0AD70ampHyaiHb0376 JW5052 cytoplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein [25664544][22001512]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane PeriplasmicSURAyesyesY220.613SpI2116.801-00Yn5-16c21/22oPeriplasmic941
YGAU_ECOLIP0ADE6ygaUyzzMb2665 JW2640 cytoplasmic peripheral IM protein, Potassium binding Kbp YgaUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane [12175925]. Homologous to OsmY.[21541338] [23230279] [12878000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN100.101CYT0-0.200913-00NiUnknown289
SUHB_ECOLIP0ADG4suhBssyAb2533 JW2517 cytoplasmic peripheral IM protein, Inositol-1-monophosphatase SuhBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SuhB forms a tight complex with RNA polymerase (RNA pol) that inhibits the IMPase catalytic activity of SuhB[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN210.179CYT0-0.200913-00NiCytoplasmic986
IMDH_ECOLIP0ADG7guaBguaRb2508 JW5401 cytoplasmic peripheral IM protein, Inosine-5'-monophosphate dehydrogenase GuaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Prediction of one very weak transmembrane helix.[23230279] [15919657]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN290.14CYT0-0.200913-00NiCytoplasmic949
ENTB_ECOLIP0ADI4entBentGb0595 JW0587 cytoplasmic peripheral IM protein, Enterobactin synthase component B EntBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[10692387]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN260.118CYT0-0.200913-00NiCytoplasmic865
YIEE_ECOLIP0ADM8yieEb3712 JW3690 cytoplasmic peripheral IM protein, YieECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN250.109CYT0-0.200913-00NiUnknown27
YIGI_ECOLIP0ADP2yigIb3820 JW5588 cytoplasmic peripheral IM protein, YigICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected), Soluble IM Fraction +CytoplasmicyesyesN280.137CYT0-0.200913-00NiUnknown29
ZAPA_ECOLIP0ADS2zapAygfEb2910 JW2878 cytoplasmic peripheral IM protein, Cell division ZapACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Binds to the FtsZ ring. ZapA interacts directly with itself, FtsN, FtsZ and FtsI. Peripheral inner membrane protein via binding to the FtsZ ring. ZapA orthologue, YgfE, binds FtsZ polymers and promotes FtsZ polymer bundling. [12368265][20487275] [20497333] [17428494] [12368265]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN670.152CYT0-0.200913-00NiUnknown297
YGIB_ECOLIP0ADT2ygiBb3037 JW5927 cytoplasmic peripheral IM protein, UPF0441 YgiBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein [25664544][20545840] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN360.178SpII369.72422Pos+2=E00Yn21-32c37/38oPeriplasmic9
TRMJ_ECOLIP0AE01trmJyfhQb2532 JW2516 cytoplasmic peripheral IM protein, tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN630.12CYT0-0.200913-00NiCytoplasmic978
AHPC_ECOLIP0AE08ahpCb0605 JW0598 cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase C AhpCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Disulfide bridge (47 47;166 166) Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN490.111CYT0-0.200913-00NiCytoplasmic1090
MAP1_ECOLIP0AE18mapb0168 JW0163 cytoplasmic peripheral IM protein, Methionine aminopeptidase MapCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][23230279] [17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN220.108CYT0-0.200913-00NiCytoplasmic963
CPXR_ECOLIP0AE88cpxRyiiAb3912 JW3883 cytoplasmic nucleoid peripheral IM protein, transcriptional regulator CpxRCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[8294007] [23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicyesyesN320.14CYT0-0.200913-00NiCytoplasmic10118
USPA_ECOLIP0AED0uspAb3495 JW3462 cytoplasmic peripheral IM protein, Universal stress A UspACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)The Usp superfamily encompasses an ancient and conserved group of proteins that are found in bacteria, archaea,fungi, flies and plants. Usually each organism has several copies of Usp genes. Escherichia coli contains six Usp proteins, which can be divided into two subfamilies on the basis of sequence similarity. [19101563] Three of these proteins belong to the UspA subfamily (UspA, UspC, and UspD), and two to the UspFG subfamily (UspF and UspG). Another homologue is YbdQ. UspC binds to the membrane-integrated sensor kinase KdpD involved in K+ ion sensing. The same could be true for the other Usp proteins but we could not find direct evidence in the literature. [1453957] [8506346] [8740179] [9405142] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15911532] [19101563] [1453957] [8506346] [8740179] [9405142] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmic (Experimental)yesyesN330.153CYT0-0.200913-00NiCytoplasmic1080
FABG_ECOLIP0AEK2fabGb1093 JW1079 cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) reductase FabGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN230.249CYT0-0.200913-00NiCytoplasmic993
FABI_ECOLIP0AEK4fabIenvMb1288 JW1281 cytoplasmic peripheral IM protein, Enoyl-(acyl-carrier-protein) reductase (NADH) FabICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN280.245CYT0-0.200913-00NiCytoplasmicMembrane1048
GYRA_ECOLIP0AES4gyrAhisW nalA parDb2231 JW2225 cytoplasmic nucleoid peripheral IM protein, DNA gyrase subunit A GyrACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicTFyesyesN360.106CYT0-0.200913-00NiCytoplasmic949
GYRB_ECOLIP0AES6gyrBacrB cou himB hisU nalC parA pcbAb3699 JW5625 cytoplasmic nucleoid peripheral IM protein, DNA gyrase subunit B GyrBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicTFyesyesN540.109CYT0-0.200913-00NiCytoplasmic9
MIND_ECOLIP0AEZ3minDb1175 JW1164 cytoplasmic peripheral IM protein, Septum site-determining MinD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10096083] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN250.128CYT0-0.200913-00NiCytoplasmicMembrane1095
MOAB_ECOLIP0AEZ9moaBchlA2b0782 JW0765 cytoplasmic peripheral IM protein, Molybdenum cofactor biosynthesis B MoaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN240.114CYT0-0.200913-00NiCytoplasmic9105
RRAB_ECOLIP0AF90rraByjgDb4255 JW4212 cytoplasmic nucleoid peripheral IM protein, Regulator of ribonuclease activity B RraBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Negative regulator of RNaseE. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicyesyesN410.107CYT0-0.200913-00NiCytoplasmic8
RIDA_ECOLIP0AF93ridAyjgFb4243 JW5755 cytoplasmic peripheral IM protein, 2-iminobutanoate/2-iminopropanoate deaminase RidACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN210.115CYT0-0.200913-00NiCytoplasmic975
NUOB_ECOLIP0AFC7nuoBb2287 JW5875 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit B NuoBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Crystal structure of the entire respiratory complex I [23417064] Crystal structure of the entire respiratory complex I [21822288] [7607227] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19189973] [15037611] [23417064] [21822288] [7607227] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmic (Experimental)yesyesN620.1CYT0-0.200913-00NiCytoplasmicMembrane978
NUOE_ECOLIP0AFD1nuoEb2285 JW2280 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit E NuoECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex[19737520]. Thermus homologue: Q56221 (NQO2_THET8). Copurifies with trigger factor in pull down assays[19737520]. [11416161] [7607227] [7690854] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23543743] [11416161] [7607227] [7690854] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)TFyesyesN670.103CYT0-0.200913-00NiCytoplasmic931
NUOI_ECOLIP0AFD6nuoIb2281 JW2276 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit I NuoICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) [21822288] [23417064] [15037611] [19189973] [11416161] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)yesyesN270.17CYT0-0.200913-01No6-26iCytoplasmic972
NUSA_ECOLIP0AFF6nusAb3169 JW3138 cytoplasmic nucleoid peripheral IM protein, Transcription termination/antitermination NusA Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Fluorescent fusions of NusA have been monitored via fluorescence microscopy. NusA was found to be located predominantly in two clusters associated with each nucleoid. NusA can self-assemble into oligomers under heat shock conditions. NusA also acquires chaperone activity.[17272300] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicyesyesN410.104CYT0-0.200913-00NiCytoplasmic999
NUSG_ECOLIP0AFG0nusGb3982 JW3945 cytoplasmic peripheral IM protein, Transcription termination/antitermination NusG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN260.108CYT0-0.200913-00NiCytoplasmic972
ODO1_ECOLIP0AFG3sucAb0726 JW0715 cytoplasmic peripheral IM protein, 2-oxoglutarate dehydrogenase E1 component SucACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279] [15919657]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN240.133CYT0-0.200913-00NiCytoplasmic96
ODO2_ECOLIP0AFG6sucBb0727 JW0716 cytoplasmic peripheral IM protein, Dihydroyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex SucBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [9298646][23230279] [15919657] [9298646]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)TFyesyesN190.112CYT0-0.200913-00NiCytoplasmic9122
ODP1_ECOLIP0AFG8aceEb0114 JW0110 cytoplasmic peripheral IM protein, Pyruvate dehydrogenase E1 component AceECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)AceE is the E1 subunit of the 10MDa Escherichia coli pyruvate dehydrogenase multienzyme complex, which catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO(2) and acetate (crystal structure solved as a dimer [11955070]). The E1 subunit is present as a 24mer within the complex. AceE is also part of a very large complex partially resolved by cryoEM [16338405]. Analogous enzyme pyryvate oxidase-PoxB is membrane associated [18988747]. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18988747] [15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicTFyesyesN530.105CYT0-0.200913-00NiCytoplasmic915
PARC_ECOLIP0AFI2parCb3019 JW2987 cytoplasmic nucleoid peripheral IM protein, DNA topoisomerase 4 subunit A ParCCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Dimer of topoisomerase IV subunit A <a href="http://BioCyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10686-MONOMER">(bioCyc)</a> Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1334483] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACell membrane; Peripheral membrane proteinCytoplasmicyesyesN180.118CYT0-0.200913-00NiCytoplasmicMembrane1017
PDXH_ECOLIP0AFI7pdxHb1638 JW1630 cytoplasmic peripheral IM protein, Pyridoxine/pyridoxamine 5'-phosphate oxidase PdxHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN430.121CYT0-0.200913-00NiCytoplasmic951
PHOB_ECOLIP0AFJ5phoBb0399 JW0389 cytoplasmic nucleoid peripheral IM protein, Phosphate regulon transcriptional regulatory PhoB Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)PhoB is a dual transcription regulator that activates expression of the Pho regulon in response to environmental Pi. Under phosphate limitation conditions the inner membrane sensor kinase PhoR autophosphorylates; Subsequent transfer of the phosphate group to PhoB results in activation of PhoB. It has been shown that PhoBR and PhoB are responsible for upregulation of phosphonate and glycerol phosphate metabolism and the high-affinity phosphate transport system, respectively, thus showing the complex regulation by the PhoR-PhoB two-component regulatory system.[2693738] [18031348] [23138451]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmCytoplasmicyesyesN330.201CYT0-0.200913-00NiCytoplasmic9
PPX_ECOLIP0AFL6ppxb2502 JW2487 cytoplasmic peripheral IM protein, Exopolyphosphatase PpxCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell membrane CytoplasmicyesyesN290.112CYT0-0.200913-00NiCytoplasmic1047
PSPA_ECOLIP0AFM6pspAb1304 JW1297 cytoplasmic peripheral IM protein, Phage shock A PspACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Forms large oligomers and binds directly to phospholipids (preferentially phosphatidylserine and phosphatidylglycerol). PMC3709507 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19555453] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicProphage TransposaseyesnoN230.112CYT0-0.200913-00NiCytoplasmicMembrane10113
YBBO_ECOLIP0AFP4ybbOb0493 JW0482 cytoplasmic nucleoid peripheral IM protein, Oxidoreductase YbbOCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCytoplasmicnoyesN450.12CYT0-0.200913-01No240-258iCytoplasmic9
PSPD_ECOLIP0AFV8pspDb1307 JW1300 cytoplasmic peripheral IM protein, Phage shock D PspDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12562786] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicProphage TransposasenonoN640.182CYT0-0.200913-00NiUnknown234
SEQA_ECOLIP0AFY8seqAb0687 JW0674 cytoplasmic peripheral IM protein, Negative modulator of initiation of replication SeqACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Membrane-associated oriC-binding machinery of E. coli is a multiprotein complex that includes the SeqA and SeqB proteins. [9660922] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9736699] [9660922] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN430.12CYT0-0.200913-00NiCytoplasmic813
SSPB_ECOLIP0AFZ3sspBb3228 JW3197 cytoplasmic peripheral IM protein, Stringent starvation B SspBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN470.112CYT0-0.200913-00NiCytoplasmic8
PUR1_ECOLIP0AG16purFb2312 JW2309 cytoplasmic peripheral IM protein, Amidophosphoribosyltransferase PurFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN320.119CYT0-0.200913-00NiCytoplasmic915
RELA_ECOLIP0AG20relAb2784 JW2755 cytoplasmic peripheral IM protein, GTP pyrophosphokinase RelACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN370.119CYT0-0.200913-00NiCytoplasmic875
RHO_ECOLIP0AG30rhonitA psuA rnsC sbaA tsub3783 JW3756 cytoplasmic peripheral IM protein, Transcription termination factor Rho Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN440.103CYT0-0.200913-00NiCytoplasmic9
SECB_ECOLIP0AG86secBb3609 JW3584 cytoplasmic peripheral IM protein, Protein-export SecBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Cytoplasmic protein that forms transient interactions with the membrane; binds to the C-terminus of SecA. Protein that acts as its membrane receptor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9767586] [2170023] [PMC1326294] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN530.133CYT0-0.200913-00NiCytoplasmic1088
RPOE_ECOLIP0AGB6rpoEsigEb2573 JW2557 cytoplasmic nucleoid peripheral IM protein, ECF RNA polymerase sigma-E factor RpoECytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity)SigmaE controls the ??E-dependent extracytoplasmic stress response and is required to maintain cell envelope integrity in Escherichia coli [9159523] [18253509]. RseA, encodes an anti-sigma(E) activity demonstrated at both the genetic and biochemical levels [9159522]. RseC is an inner membrane protein that positively modulates sigma(E) activity [9159522]. ??E is held at the membrane by the antisigma factor RseA. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB binds to the periplasmic domain of RseA and protects RseA from proteolysis. [17360428]. Unfolded OMPs activate the protease DegS, which cleave RpoE regulon [15520285]. Identified in the inner membrane by biochemical followed by two-dimensional gel electrophoresis and tandem mass spectrometry [15911532]. [15774872] [16597971] [11777003] Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase. [12718891] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9159522] [15911532] [20971899] [23138451] [15520285] [7889934] [17360428] [11777003] [15774872] [16597971] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)yesyesN440.111CYT0-0.200913-00NiCytoplasmic10101
SRP54_ECOLIP0AGD7ffhb2610 JW5414 cytoplasmic peripheral IM protein, Signal recognition particle FfhCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18281057] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN630.1CYT0-0.200913-00NiUnknown5106
SUCD_ECOLIP0AGE9sucDb0729 JW0718 cytoplasmic peripheral IM protein, Succinate--CoA ligase (ADP-forming) subunit alpha SucDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN310.171CYT0-0.200913-00NiCytoplasmic970
TDCB_ECOLIP0AGF6tdcBb3117 JW3088 cytoplasmic peripheral IM protein, L-threonine dehydratase catabolic TdcB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential substrate of periplasmic chaperone YfgM. [25403562] Putative substrate for the periplasmic chaperone YfgM. [25403562] [25403562]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicYfgMyesyesN230.152CYT0-0.200913-00NiCytoplasmic972
TRKA_ECOLIP0AGI8trkAb3290 JW3251 cytoplasmic peripheral IM protein, Trk system potassium uptake TrkA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[2674131] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicnoyesN170.13CYT0-0.200913-00Yn3-11c16/17oCytoplasmicMembrane1068
YFIF_ECOLIP0AGJ5yfiFb2581 JW2565 cytoplasmic peripheral IM protein, tRNA/rRNA methyltransferase YfiF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFnoyesN450.103CYT0-0.200913-00NiCytoplasmic981
SYY_ECOLIP0AGJ9tyrSb1637 JW1629 cytoplasmic peripheral IM protein, Tyrosine--tRNA ligase TyrSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation unit[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicnoyesN650.123CYT0-0.200913-00NiCytoplasmic1063
RSME_ECOLIP0AGL7rsmEyggJb2946 JW2913 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase E RsmECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Ribosomal[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cytoplasm CytoplasmicyesyesN400.129CYT0-0.200913-00NiCytoplasmic818
RLME_ECOLIP0C0R7rlmEftsJ mrsF rrmJb3179 JW3146 cytoplasmic peripheral IM protein, Ribosomal RNA large subunit methyltransferase E RlmECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN290.111CYT0-0.200913-00NiCytoplasmic916
GATY_ECOLIP0C8J6gatYyegFb2096 JW5343 cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN490.137CYT0-0.200913-00NiCytoplasmic911
GATZ_ECOLIP0C8J8gatZb2095 JW2082 cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN330.318CYT0-0.200913-00NiCytoplasmic86
EFTU1_ECOLIP0CE47tufAb3339 JW3301 cytoplasmic peripheral IM protein, Elongation factor Tu 1 TufACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. [7525272] [7021545] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451] Disulfide bond-containing protein [25664544][20133608] [2022614] [775340] [7525272] [7021545] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm Cell inner membrane; Peripheral membrane protein Cytoplasmic (Experimental)yesyesN470.197CYT0-0.200913-00NiCytoplasmic1099
EFTU2_ECOLIP0CE48tufBb3980 JW3943 cytoplasmic peripheral IM protein, Elongation factor Tu 2 TufBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[2022614] [775340] [23230279] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cell inner membrane; Peripheral membrane protein CytoplasmicyesyesN470.197CYT0-0.200913-00NiCytoplasmic1087
RCSB_ECOLIP0DMC7rcsBb2217 JW2205 cytoplasmic nucleoid peripheral IM protein, transcriptional regulator RcsBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Regulator protein RcsB is a member of the two-component regulatory system RcsC/RcsB. It is involved in the activation of colanic acid capsule synthesis (cps) and cell division (ftsZ) genes. It is believed that the RcsB protein is activated through phosphorylation, probably by the sensor protein RcsC. RcsB protein for Regulator capsule synthesis B, is a response regulator that belongs to the multicomponent RcsF/RcsC/RcsD/RcsA-RcsB phosphorelay system. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). [2404948] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicnoyesN660.117CYT0-0.200913-00NiCytoplasmic1090
FTSY_ECOLIP10121ftsYb3464 JW3429 cytoplasmic peripheral IM protein, Signal recognition particle receptor FtsY Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Contains an essential and autonomous membrane-binding amphipathic helix. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18281057] [8194520] [9177162] [11353766] [17726013] [19414018] [21543314] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN30.107CYT0-0.200913-00NiCytoplasmicMembrane10
GLNQ_ECOLIP10346glnQb0809 JW0794 cytoplasmic peripheral IM protein, Glutamine transport ATP-binding GlnQ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)yesyesN420.105CYT0-0.200913-00NiCytoplasmicMembrane1097
ENTE_ECOLIP10378entEb0594 JW0586 cytoplasmic peripheral IM protein, Enterobactin synthase component E EntECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous to FadD13 protein of Mycobacterium that is peripheral. Phobius predits transmembrane region around aa100. Binds AMP. Very high homology to eight other transport proteins: (FADK_ECOLI, CAIC_ECOLI, LCFA_ECOLI, ENTF_ECOLI, ACSA_ECOLI) Membrane Association of the Escherichia coli Enterobactin Synthase Proteins EntB/G, EntE, and EntF[10692387]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/AMembrane CytoplasmicyesyesN360.15CYT0-0.200913-01No86-105iCytoplasmic988
SECA_ECOLIP10408secAazi pea prlDb0098 JW0096 cytoplasmic peripheral IM protein, translocase subunit SecA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm CytoplasmicnoyesN700.109CYT0-0.200913-00NiCytoplasmicMembrane1058
LPXB_ECOLIP10441lpxBpgsBb0182 JW0177 cytoplasmic peripheral IM protein, Lipid-A-disaccharide synthase LpxBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer[19883124]. It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer. [19883124] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19883124] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN230.146CYT0-0.200913-00NiCytoplasmic1047
UGPC_ECOLIP10907ugpCb3450 JW3415 cytoplasmic peripheral IM protein, sn-glycerol-3-phosphate import ATP-binding UgpC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN370.105CYT0-0.200913-00NiCytoplasmicMembrane1020
LIT_ECOLIP11072litb1139 JW1125 cytoplasmic peripheral IM protein, Cell death peptidase LitSingle weak hydrophobic region. Could be a transmembrane domain but could also be a peripheral inner membrane protein.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane CytoplasmicProphage Integrase (EcoGene)nonoN200.181CYT0-0.200913-01Ni61-81oCytoplasmic8
ARLY_ECOLIP11447argHb3960 JW3932 cytoplasmic peripheral IM protein, Argininosuccinate lyase ArgHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN530.138CYT0-0.200913-00NiCytoplasmic9
NARH_ECOLIP11349narHb1225 JW1216 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 1 beta chain NarHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. [12910261] In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. [19295650] When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. [2674654] The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. [3371362] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] [3053688] [2233673] [2674654] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12910261] [3053688] [2233673] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell membrane; Peripheral membrane proteinCytoplasmic (Experimental)yesyesN320.117CYT0-0.200913-00NiCytoplasmicMembrane1013
ENTF_ECOLIP11454entFb0586 JW0578 cytoplasmic peripheral IM protein, Enterobactin synthase component F EntFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[10692387]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN560.235CYT0-0.200913-00NiCytoplasmic920
BGLG_ECOLIP11989bglGbglCb3723 JW3701 cytoplasmic peripheral IM protein, Cryptic beta-glucoside bgl operon antiterminator BglGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)BglG is a transcriptional antiterminator which controls the expression bgl operon. It has been detected at the membrane when it is expressed with BglF, when expressed as monocistron, the bglG transcripts were observed at the cell poles (unpublished data). In the absence of ??-glucosidase, BglG also assembles near membrane and forms a pro-form complex with BglF that maintains BglG inactive. In the presence of sugar, BglG associates with the proteins of the sugar phosphotransfer system (PTS) and localizes at the cell poles. BglG requires two distinct (de)phosphorylation events to gain activity.[22984181]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN380.103CYT0-0.200913-00NiCytoplasmic848
METH_ECOLIP13009metHb4019 JW3979 cytoplasmic peripheral IM protein, Methionine synthase MetHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN350.124CYT0-0.200913-00NiCytoplasmic9
GLPB_ECOLIP13033glpBb2242 JW2236 cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit B GlpBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. [6387427] Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. [1987111] [PMC3210124] [3286606][20706981] [6387427] [1987111] [PMC3210124] [3286606]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane; Peripheral membrane protein Cytoplasmic (Experimental)yesyesN300.137CYT0-0.200913-00Yn10-21c28/29oCytoplasmicMembrane1024
GLPD_ECOLIP13035glpDglyDb3426 JW3389 cytoplasmic peripheral IM protein, Aerobic glycerol-3-phosphate dehydrogenase GlpDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Binds to the cytoplasmic membrane through an amphipathic C-terminal alpha-helix (357-374)[11955283]. Identified in PlsB-PflB-GlpD-Psd [20521846]. Detergent-depleted enzyme can be functionally reconstituted with Escherichia coli membrane vesicles to support glycerol-3-phosphate-dependent active transport of L-proline[340460]. When GlpD is lost by mutation, the cells not only fail to grow on glycerol or l-alpha-glycerophosphate, but are subject to growth inhibition in the presence of either compound [5321485]. Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. [1987111] GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. [6387427] Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [8982270] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11955283] [340460] [15919657] [15911532] [8982270] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmic (Experimental)yesyesN350.138CYT0-0.200913-00NiCytoplasmic1048
RPOS_ECOLIP13445rpoSappR katF nur otsX sigSb2741 JW5437 cytoplasmic nucleoid peripheral IM protein, RNA polymerase sigma factor RpoS Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm CytoplasmicnoyesN690.1CYT0-0.200913-00NiCytoplasmic1030
PROV_ECOLIP14175proVb2677 JW2652 cytoplasmic peripheral IM protein, Glycine betaine/proline betaine transport system ATP-binding ProV Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN240.108CYT0-0.200913-00NiCytoplasmicMembrane1073
FECE_ECOLIP15031fecEb4287 JW4247 cytoplasmic peripheral IM protein, Fe(3+) dicitrate transport ATP-binding FecECytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN620.148CYT0-0.200913-00NiCytoplasmicMembrane104
FLIN_ECOLIP15070fliNflaN motDb1946 JW1930 Flagellar cytoplasmic peripheral IM protein, motor switch FliN Flaggelar C-ring proteins - FliG, FliM, and FliN.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane; Peripheral membrane protein; Cytoplasmic side Bacterial flagellum basal bodyCytoplasmicflagellumnoyesN350.119CYT0-0.200913-00NiCytoplasmicMembrane10107
MALQ_ECOLIP15977malQmalAb3416 JW3379 cytoplasmic peripheral IM protein, 4-alpha-glucanotransferase MalQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN230.214SpI220.437923-00NiCytoplasmic1031
SDHL_ECOLIP16095sdaAb1814 JW1803 cytoplasmic peripheral IM protein, L-serine dehydratase 1 SdaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN200.136CYT0-0.200913-00NiCytoplasmic971
HYCE_ECOLIP16431hycEhevEb2721 JW2691 cytoplasmic peripheral IM protein, Formate hydrogenlyase subunit 5 HycECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Nickel-containing large subunit of Hydrogenase 3 (hycABCDEF-GHI) that forms a loose association with the molyb- doenzyme formate dehydrogenase-H on the cytoplasmic face of the cytoplasmic membrane, where together they make up the formate hydrogenlyase complex.[1625581]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN270.119CYT0-0.200913-00NiCytoplasmic97
SYP_ECOLIP16659proSdrpAb0194 JW0190 cytoplasmic peripheral IM protein, Proline--tRNA ligase ProSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicTFyesyesN190.254CYT0-0.200913-00NiCytoplasmic1016
CYSA_ECOLIP16676cysAb2422 JW2415 cytoplasmic peripheral IM protein, Sulfate/thiosulfate import ATP-binding CysA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN330.112CYT0-0.200913-00NiCytoplasmicMembrane714
PHNC_ECOLIP16677phnCb4106 JW4067 cytoplasmic peripheral IM protein, Phosphonates import ATP-binding PhnCCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN230.137CYT0-0.200913-00NiCytoplasmicMembrane1010
PHNK_ECOLIP16678phnKb4097 JW5727 cytoplasmic peripheral IM protein, Putative phosphonates utilization ATP-binding PhnK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN230.117CYT0-0.200913-00NiCytoplasmicMembrane76
PHNL_ECOLIP16679phnLb4096 JW4057 cytoplasmic peripheral IM protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN340.263CYT0-0.200913-00NiCytoplasmicMembrane98
PHNP_ECOLIP16692phnPb4092 JW4053 cytoplasmic peripheral IM protein, Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase PhnPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[8335257]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/APeriplasmic (Experimental)yesyesN210.153CYT0-0.200913-00Yn3-10c15/16oCytoplasmic9
RHSB_ECOLIP16917rhsBb3482 JW5679 cytoplasmic peripheral IM protein, RhsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicRhs ElementnonoN460.116CYT0-0.200913-00NiExtracellular9
GLMS_ECOLIP17169glmSb3729 JW3707 cytoplasmic peripheral IM protein, Glutamine--fructose-6-phosphate aminotransferase GlmSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmCytoplasmicTFnoyesN590.122CYT0-0.200913-00NiCytoplasmic1091
MURG_ECOLIP17443murGb0090 JW0088 cytoplasmic peripheral IM protein, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase MurGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Pyrophosphoryl-undecaprenol N-acetylglucosamine transferase. Murein synthesis peripheral membrane protein interacting with cardiolipin. [8449890] [10892798] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][8449890] [10892798] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane Membrane associated (Experimental)yesyesN270.156TMH03.61924-00NiCytoplasmicMembrane1048
BETA_ECOLIP17444betAb0311 JW0303 cytoplasmic peripheral IM protein, Oxygen-dependent choline dehydrogenase BetACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3512525] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane CytoplasmicnoyesN190.163CYT0-0.200913-00NiCytoplasmicMembrane108
CYSH_ECOLIP17854cysHb2762 JW2732 cytoplasmic peripheral IM protein, Phosphoadenosine phosphosulfate reductase CysHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN590.168CYT0-0.200913-00NiCytoplasmic1011
MURC_ECOLIP17952murCb0091 JW0089 cytoplasmic peripheral IM protein, UDP-N-acetylmuramate--L-alanine ligase MurCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM Fraction +Cytoplasm CytoplasmicTFyesyesN410.228CYT0-0.200913-00NiCytoplasmic976
NADE_ECOLIP18843nadEefg ntrLb1740 JW1729 cytoplasmic peripheral IM protein, NH(3)-dependent NAD(+) synthetase NadECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN200.146CYT0-0.200913-00NiCytoplasmic9
NARY_ECOLIP19318narYb1467 JW1462 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 2 beta chain NarYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane; Peripheral membrane proteinCytoplasmicyesyesN320.124CYT0-0.200913-00NiCytoplasmicMembrane1014
NARZ_ECOLIP19319narZb1468 JW1463 cytoplasmic peripheral IM protein, Respiratory nitrate reductase 2 alpha chain NarZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell membrane; Peripheral membrane proteinCytoplasmicyesyesN200.145CYT0-0.200913-00NiCytoplasmicMembrane10
FADB_ECOLIP21177fadBoldBb3846 JW3822 cytoplasmic peripheral IM protein, Fatty acid oxidation complex subunit alpha FadBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN310.13CYT0-0.200913-00NiCytoplasmic915
RNR_ECOLIP21499rnrvacB yjeCb4179 JW5741 cytoplasmic peripheral IM protein, Ribonuclease R RnrCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN30.1CYT0-0.200913-00NiCytoplasmic945
SRMB_ECOLIP21507srmBrbaB rhlAb2576 JW2560 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase SrmBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associate with the 50S subunit.[23230279] [15919657]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN330.109CYT0-0.200913-00NiCytoplasmic1068
RNE_ECOLIP21513rneams hmp1b1084 JW1071 cytoplasmic peripheral IM protein, Ribonuclease E RneCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Membrane-binding specificity of NRne was confirmed using circular dichroism difference spectroscopy. RnE binds specifically to anionic phospholipids through electrostatic interactions through N-terminal region (NRne, residues 1-499). Identified DsbC substrate [19953543] Identified DsbA substrate [19953543] [2011493] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][18976283] [15911532] [22509045] [2011493] [23230279] [24438330]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)yesyesN620.101CYT0-0.200913-00NiCytoplasmic10
KPYK2_ECOLIP21599pykAb1854 JW1843 cytoplasmic peripheral IM protein, Pyruvate kinase II PykACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN430.131CYT0-0.200913-00NiCytoplasmic943
DBPA_ECOLIP21693dbpAb1343 JW1337 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase DbpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; Fragment of 23S rRNA. Assembly cofactor of ribosome; Fragment of 23S rRNA. [21529161][21529161]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN320.341CYT0-0.200913-00NiCytoplasmic912
SYD_ECOLIP21889aspStlsb1866 JW1855 cytoplasmic peripheral IM protein, Aspartate--tRNA ligase AspSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SecY-interacting protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19139097] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN270.104CYT0-0.200913-00NiCytoplasmic1081
ASNB_ECOLIP22106asnBb0674 JW0660 cytoplasmic peripheral IM protein, Asparagine synthetase B AsnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN370.141CYT0-0.200913-00NiCytoplasmic9
CYSQ_ECOLIP22255cysQamtAb4214 JW4172 cytoplasmic peripheral IM protein, 3'(2'),5'-bisphosphate nucleotidase CysQCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [15911532][15911532]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane Membrane associated (Experimental)noyesN400.152CYT0-0.200913-00NiCytoplasmic983
GABT_ECOLIP22256gabTb2662 JW2637 cytoplasmic peripheral IM protein, 4-aminobutyrate aminotransferase GabTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN650.16CYT0-0.200913-00NiCytoplasmic977
ADD_ECOLIP22333addb1623 JW1615 cytoplasmic peripheral IM protein, Adenosine deaminase AddCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN400.118CYT0-0.200913-00NiCytoplasmic915
MUKB_ECOLIP22523mukBb0924 JW0907 cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukBCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)[16982609] [20921377] [11886550] [22380631] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN460.13CYT0-0.200913-00NiCytoplasmic10
MUKE_ECOLIP22524mukEkicA ycbAb0923 JW0906 cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukECytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[16982609] [20921377] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN250.113CYT0-0.200913-00NiCytoplasmic985
LIVF_ECOLIP22731livFb3454 JW3419 cytoplasmic peripheral IM protein, High-affinity branched-chain amino acid transport ATP-binding LivF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN420.111CYT0-0.200913-00NiCytoplasmicMembrane715
FECI_ECOLIP23484fecIb4293 JW4253 cytoplasmic nucleoid peripheral IM protein, Probable RNA polymerase sigma factor FecI Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Interacts with FecR at the inner membrane propably through the N-terminal region of FecR (1-85) which is cytoplasmic[16718597]. FecR spans the cytoplasmic membrane once, interacts in the periplasm with its C-terminal region with FecA occupied by ferric citrate, and transmits the information through the cytoplasmic membrane into the cytoplasm, where it converts FecI into an active sigma factor[9573190]. FecI specifically binds FecR (a transmembrane protein) and that this binding is necessary for FecI to function as a sigma factor[12057967]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12057967] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicnoyesN230.11CYT0-0.200913-00NiCytoplasmic1016
PPSA_ECOLIP23538ppsAppsb1702 JW1692 cytoplasmic peripheral IM protein, Phosphoenolpyruvate synthase PpsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)One very weak transmembrane region predicted. Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN560.169CYT0-0.200913-00NiCytoplasmic948
PSS_ECOLIP23830pssApssb2585 JW2569 cytoplasmic peripheral IM protein, CDP-diacylglycerol--serine O-phosphatidyltransferase PssACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid metabolism complex PlsB-PssA-YbgC-Acp. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm Cell inner membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmicTFyesyesN480.108CYT0-0.200913-00NiCytoplasmic1035
PPIB_ECOLIP23869ppiBb0525 JW0514 cytoplasmic peripheral IM protein, Peptidyl-prolyl cis-trans isomerase B PpiBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [9268657]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmic (Experimental)yesyesN100.101CYT0-0.200913-00NiCytoplasmic1064
HEMH_ECOLIP23871hemHpopA visAb0475 JW0464 cytoplasmic peripheral IM protein, Ferrochelatase HemHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN630.136CYT0-0.200913-00NiCytoplasmic1015
FEPC_ECOLIP23878fepCb0588 JW0580 cytoplasmic peripheral IM protein, Ferric enterobactin transport ATP-binding FepC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN630.171CYT0-0.200913-00NiCytoplasmicMembrane917
GSA_ECOLIP23893hemLgsa popCb0154 JW0150 cytoplasmic peripheral IM protein, Glutamate-1-semialdehyde 2,1-aminomutase HemLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN260.222CYT0-0.200913-00NiCytoplasmic933
ARGE_ECOLIP23908argEb3957 JW3929 cytoplasmic peripheral IM protein, Acetylornithine deacetylase ArgECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN230.163CYT0-0.200913-00NiCytoplasmic942
RFAC_ECOLIP24173rfaCrfa-2 waaC yibCb3621 JW3596 cytoplasmic peripheral IM protein, polysaccharide heptosyltransferase 1 RfaCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN500.195CYT0-0.200913-00NiCytoplasmic951
ACCC_ECOLIP24182accCfabGb3256 JW3224 cytoplasmic peripheral IM protein, Biotin carboxylase AccCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation [19737520].[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN360.117CYT0-0.200913-00NiCytoplasmic929
RADA_ECOLIP24554radAsmsb4389 JW4352 cytoplasmic nucleoid peripheral IM protein, DNA repair RadACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmicyesyesN410.199CYT0-0.200913-00NiCytoplasmicMembrane929
FRMA_ECOLIP25437frmAadhCb0356 JW0347 cytoplasmic peripheral IM protein, S-(hydroxymethyl)glutathione dehydrogenase FrmACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmCytoplasmicyesyesN500.109CYT0-0.200913-00NiCytoplasmic1071
HFLX_ECOLIP25519hflXb4173 JW4131 cytoplasmic peripheral IM protein, GTPase HflX Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The hflALocus was found to comprise three genes, hflX, hflK, and hflC. HflK and HflC are membrane proteins and form a mem- brane-bound protease complex along with FtsH(HflB), and they are thought to act as modulators of the function of HflB. The role of the HflX GTPase/ATPase is unclear but has been shown to bind to ribosomes. When overexpressed, a portion of HflXProtein appeared in the membrane fraction, while larger amounts fractionated with the cytoplasmic pool (unpublished data cited in [19181811]). In Corynebacterium glutamicum, HflX has been found to occur equally in the cytoplasm and in the membrane fractions ([15978086]). Has no transmembrane region either in E. coli or in C. glutamicum. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19181811] [15978086] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN520.116CYT0-0.200913-00NiCytoplasmic852
MNME_ECOLIP25522mnmEthdF trmEb3706 JW3684 cytoplasmic peripheral IM protein, tRNA modification GTPase MnmE Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)A GTPase that exhibits high intrinsic GTPase hydrolysis rate,Low affinity for GTP, and extremely low affinity for GDP Can form self-assemblies. Is a cytoplasmic protein partially associated with the inner membrane. Involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs. Essential for viability depending on genetic background. Fluorescent fusions of MnmE have been monitored via fluorescence microscopy. MnmE located predominantly beside the membrane.[10601028] [17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN470.153CYT0-0.200913-00NiCytoplasmic989
GABD_ECOLIP25526gabDb2661 JW2636 cytoplasmic peripheral IM protein, Succinate-semialdehyde dehydrogenase GabDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN280.124CYT0-0.200913-00NiCytoplasmic960
UBIH_ECOLIP25534ubiHvisBb2907 JW2875 cytoplasmic peripheral IM protein, 2-octaprenyl-6-methoxyphenol hydroxylase UbiHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)The biosynthesis of UQ is studied in great detail in bacteria and yeast but only to a limited extent in animal tissues. The UQ biosynthetic enzymes may constitute a complex that is tightly bound to the membrane.[11583838]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN420.38SpI251.25365-00Yn3-13c18/19oUnknown615
HTRL_ECOLIP25666htrLyibBb3618 JW5644 cytoplasmic peripheral IM protein, HtrLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN450.131CYT0-0.200913-00NiCytoplasmic850
RFAG_ECOLIP25740rfaGpcsA waaGb3631 JW3606 cytoplasmic peripheral IM protein, polysaccharide core biosynthesis RfaGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN250.118CYT0-0.200913-00NiCytoplasmic930
RFAP_ECOLIP25741rfaPwaaPb3630 JW3605 cytoplasmic peripheral IM protein, polysaccharide core heptose RfaPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN200.155CYT0-0.200913-00NiCytoplasmic933
RFAQ_ECOLIP25742rfaQwaaQb3632 JW3607 cytoplasmic peripheral IM protein, polysaccharide core heptosyltransferase RfaQ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN250.11CYT0-0.200913-00NiCytoplasmic833
HFLD_ECOLIP25746hflDycfCb1132 JW5165 cytoplasmic peripheral IM protein, High frequency lysogenization HflDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Downregulates lambda lysogenization;Interacts with cIIProtein; first gene in purB operon Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11278968] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN540.157CYT0-0.200913-00NiUnknown219
RHLE_ECOLIP25888rhlEb0797 JW0781 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase RhlECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associates with the 70S subunit. Overex<x>pression of protein suppresses defects in deletion of DeaD. [18083833][23230279] [18083833]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN460.14CYT0-0.200913-00NiCytoplasmic962
WAAU_ECOLIP27242waaUrfaK waaKb3623 JW3598 cytoplasmic peripheral IM protein, polysaccharide 1,2-N-acetylglucosaminetransferase WaaUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[1657881]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/AMembrane; Peripheral membrane proteinCytoplasmicnoyesN330.113CYT0-0.200913-00NiCytoplasmic8
PLSX_ECOLIP27247plsXb1090 JW5156 cytoplasmic peripheral IM protein, Phosphate acyltransferase PlsXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN240.213CYT0-0.200913-00NiCytoplasmic915
GCST_ECOLIP27248gcvTb2905 JW2873 cytoplasmic peripheral IM protein, Aminomethyltransferase GcvTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN310.116CYT0-0.200913-00NiCytoplasmic9101
ARGK_ECOLIP27254argKygfDb2918 JW2885 cytoplasmic peripheral IM protein, GTPase ArgK ArgKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Appears to phosphorylate the periplasmic binding proteins of the lysine, arginine, ornithine transport system as well as the arginine, ornithine transport system[2136858].[2136858]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN260.12CYT0-0.200913-00NiCytoplasmicMembrane1023
NADR_ECOLIP27278nadRnadIb4390 JW5800 cytoplasmic nucleoid peripheral IM protein, Trifunctional NAD biosynthesis/regulator NadR Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)May loosely associate with the membrane through its interaction with the membrane-bound pnuC (Nicotinamide riboside transporter). In combination with corepressor (NAD?), the cytoplasmic form of nadR would be capable of acting as a transcriptional repressor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10464228] [23138451] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACell membrane; Peripheral membrane protein Cytoplasm CytoplasmicyesyesN390.131CYT0-0.200913-00NiCytoplasmic839
OPDA_ECOLIP27298prlCopdAb3498 JW3465 cytoplasmic peripheral IM protein, Oligopeptidase A PrlCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN260.115CYT0-0.200913-00NiCytoplasmic1094
TKT1_ECOLIP27302tktAtktb2935 JW5478 cytoplasmic peripheral IM protein, Transketolase 1 TktACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN250.124CYT0-0.200913-00NiCytoplasmic932
STHA_ECOLIP27306sthAsth udhAb3962 JW5551 cytoplasmic peripheral IM protein, Soluble pyridine nucleotide transhydrogenase SthACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SthA is bound on the inner mebrane through PntAB heterodimer. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][14660605] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicTFyesyesN220.133CYT0-0.200913-00NiCytoplasmic1022
ACSA_ECOLIP27550acsyfaCb4069 JW4030 cytoplasmic peripheral IM protein, Acetyl-coenzyme A synthetase AcsCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homologous to FadD13 protein of Mycobacterium that is peripheral. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][22560731] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN270.136CYT0-0.200913-00NiCytoplasmic962
WECG_ECOLIP27836wecGrffMb3794 JW3770 cytoplasmic peripheral IM protein, UDP-N-acetyl-D-mannosaminuronic acid transferase WecGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN270.16CYT0-0.200913-00NiCytoplasmic835
TREC_ECOLIP28904treColgHb4239 JW4198 cytoplasmic peripheral IM protein, Trehalose-6-phosphate hydrolase TreCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFyesyesN600.145CYT0-0.200913-00NiCytoplasmic109
ALR2_ECOLIP29012dadXalnB dadBb1190 JW1179 cytoplasmic peripheral IM protein, Alanine racemase, catabolic DadXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN250.206CYT0-0.200913-00NiCytoplasmic102
METN_ECOLIP30750metNabcb0199 JW0195 cytoplasmic peripheral IM protein, Methionine import ATP-binding MetN Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN420.139CYT0-0.200913-00NiCytoplasmicMembrane923
RNB_ECOLIP30850rnbb1286 JW1279 cytoplasmic nucleoid peripheral IM protein, Exoribonuclease 2 RnbCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II [23344958] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][23344958] [24438330]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasmCytoplasmicyesyesN230.181CYT0-0.200913-00NiCytoplasmic1048
MODF_ECOLIP31060modFphrAb0760 JW0743 cytoplasmic peripheral IM protein, ABC transporter ATP-binding ModFCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicnoyesN340.221CYT0-0.200913-00NiCytoplasmicMembrane933
POTG_ECOLIP31134potGb0855 JW5818 cytoplasmic peripheral IM protein, Putrescine transport ATP-binding PotG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN350.11CYT0-0.200913-00NiCytoplasmicMembrane711
THIQ_ECOLIP31548thiQyabJb0066 JW0065 cytoplasmic peripheral IM protein, Thiamine import ATP-binding ThiQ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Two very weak TM predictions; membrane associated activity known. Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system [12175925][12175925]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN250.144CYT0-0.200913-00NiCytoplasmicMembrane108
CAIC_ECOLIP31552caiCyaaMb0037 JW0036 cytoplasmic peripheral IM protein, Crotonobetaine/carnitine--CoA ligase CaiCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to FadD13 protein of Mycobacterium that is peripheral.[22560731]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN180.105CYT0-0.200913-00NiCytoplasmic9
OTSA_ECOLIP31677otsAb1896 JW5312 cytoplasmic peripheral IM protein, Trehalose-6-phosphate synthase OtsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN190.248CYT0-0.200913-00NiCytoplasmic1030
NUOF_ECOLIP31979nuoFb2284 JW2279 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit F NuoFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.cThermus homologue: Q56222 (NQO1_THET8) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN570.101CYT0-0.200913-00NiCytoplasmic911
TYPA_ECOLIP32132typAbipA yihKb3871 JW5571 cytoplasmic peripheral IM protein, GTP-binding TypA/BipA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicTFyesyesN370.112CYT0-0.200913-00NiCytoplasmicMembrane788
PTFLA_ECOLIP32155frvAyiiKb3900 JW3871 cytoplasmic peripheral IM protein, PTS system fructose-like EIIA component FrvACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Fructose-like phosphotransferase enzyme IIA component Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN190.135CYT0-0.200913-00NiCytoplasmic968
PTFX2_ECOLIP32670ptsAfrwA yijHb3947 JW5555 cytoplasmic peripheral IM protein, Multiphosphoryl transfer 2 PtsACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Multiphosphoryl transfer protein 2 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN180.56CYT0-0.200913-00NiCytoplasmic968
PTFB3_ECOLIP32676frwDyijNb3953 JW3925 cytoplasmic peripheral IM protein, PTS system fructose-like EIIB component 3 FrwDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Sequence analysis indicates that an frwD encoded protein has similarity to the IIB domains of the PTS Enzymes II specific for fructose. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN210.559CYT0-0.200913-00Yn4-12c17/18oUnknown643
RLUF_ECOLIP32684rluFyjbCb4022 JW3982 cytoplasmic peripheral IM protein, 23S rRNA pseudouridine(2604) synthase RluFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN250.109CYT0-0.200913-00NiCytoplasmic9
DUSA_ECOLIP32695dusAyjbNb4049 JW5950 cytoplasmic peripheral IM protein, tRNA-dihydrouridine(20/20a) synthase DusACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN270.108CYT0-0.200913-00NiCytoplasmic9
ALSA_ECOLIP32721alsAyjcWb4087 JW4048 cytoplasmic peripheral IM protein, D-allose import ATP-binding AlsACytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN210.291CYT0-0.200913-00NiCytoplasmicMembrane1018
GCSP_ECOLIP33195gcvPb2903 JW2871 cytoplasmic peripheral IM protein, Glycine dehydrogenase GcvPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN600.111CYT0-0.200913-01No751-770iCytoplasmic921
LLDD_ECOLIP33232lldDlctDb3605 JW3580 cytoplasmic peripheral IM protein, L-lactate dehydrogenase LldDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)LldD is a cytochrome. There is a strong experimental indication that it is a membrane bound protein although no transmembrane helix is predicted [18473]. [8407843] [6997721] [18473] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18473] [15919657] [8407843] [6997721] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)Cell inner membrane Membrane associated (Experimental)yesyesN320.129CYT0-0.200913-00NiCytoplasmic94
MLRA_ECOLIP33358mlrAyehVb2127 JW2115 cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator MlrACytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)MlrA has regulatory role in transcription activation of csgD, which is the master regulator of biofilm formation.[20874755]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1IM (predicted)CytoplasmicyesyesN230.15CYT0-0.200913-01No135-155iCytoplasmic9114
YEHX_ECOLIP33360yehXb2129 JW2117 cytoplasmic peripheral IM protein, Glycine betaine uptake system ATP-binding YehX Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN420.103CYT0-0.200913-00NiCytoplasmicMembrane78
TKT2_ECOLIP33570tktBb2465 JW2449 cytoplasmic peripheral IM protein, Transketolase 2 TktBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN240.145CYT0-0.200913-00NiCytoplasmic911
NIKD_ECOLIP33593nikDb3479 JW3444 cytoplasmic peripheral IM protein, Nickel import ATP-binding NikD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN480.168CYT0-0.200913-00NiCytoplasmicMembrane745
NIKE_ECOLIP33594nikEb3480 JW3445 cytoplasmic peripheral IM protein, Nickel import ATP-binding NikE Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN680.149CYT0-0.200913-00NiCytoplasmicMembrane941
NUOCD_ECOLIP33599nuoCnuoCD nuoDb2286 JW5375 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit C/D NuoCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [7607227] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [15919657] [7607227] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cytoplasm Cytoplasmic (Experimental)TFyesyesN390.108CYT0-0.200913-00NiCytoplasmic917
NUOG_ECOLIP33602nuoGb2283 JW2278 cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit G NuoGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. [11416161] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [15919657] [11416161] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)TFyesyesN570.143CYT0-0.200913-00NiCytoplasmicMembrane1017
YEJF_ECOLIP33916yejFb2180 JW2168 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YejFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN350.139CYT0-0.200913-00NiCytoplasmicMembrane9
CCMA_ECOLIP33931ccmAyejWb2201 JW5366 cytoplasmic peripheral IM protein, Cytochrome c biogenesis ATP-binding export CcmACytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN290.16CYT0-0.200913-00NiCytoplasmicMembrane747
MQO_ECOLIP33940mqoyojHb2210 JW2198 cytoplasmic peripheral IM protein, Malate:quinone oxidoreductase MqoCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)High signal PProbability and lipoP SPIPrediction; weak TmHMMPrediction definite membrane association according to literature. MQOIs a flavin adenine dinucleotide (FAD)- and lipid-dependent peripheral membrane protein catalyzing the oxidation ofL-malate to oxaloacetate. [11092847] [368072] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11092847] [368072] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Membrane associated (Experimental)yesyesY270.278SpI218.41159-00Yn4-15c21/22oUnknown617
AHPF_ECOLIP35340ahpFb0606 JW0599 cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase subunit F AhpFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bridge (345 348) [19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN350.108CYT0-0.200913-00NiCytoplasmicMembrane776
WBBL_ECOLIP36667wbbLb4571 JW2016 b2031cytoplasmic peripheral IM protein, Rhamnosyltransferase WbbL Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN510.111CYT0-0.200913-00NiUnknown254
ZAPD_ECOLIP36680zapDyacFb0102 JW0099 cytoplasmic peripheral IM protein, Cell division ZapDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity. 22505682[22505682] 22505682Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN390.116CYT0-0.200913-00NiCytoplasmic880
ACNB_ECOLIP36683acnByacI yacJb0118 JW0114 cytoplasmic peripheral IM protein, Aconitate hydratase B AcnBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. [10585860] Disulfide bond-containing protein [25664544][23230279] [10585860]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmic (Experimental)TFyesyesN420.124CYT0-0.200913-00NiCytoplasmic990
YADG_ECOLIP36879yadGb0127 JW0123 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YadGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN310.246CYT0-0.200913-00NiCytoplasmicMembrane75
YADI_ECOLIP36881yadIb0129 JW0125 cytoplasmic peripheral IM protein, Putative phosphotransferase enzyme IIA component YadI Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Putative phosphotransferase enzyme IIA component yadICytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN460.118CYT0-0.200913-00NiCytoplasmic817
KDPF_ECOLIP36937kdpFb4513 JW0687 cytoplasmic peripheral IM protein, Potassium-transporting ATPase KdpF subunit KdpFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)KdpF, could be identified on high resolution SDS-polyacrylamide gels in addition to the subunits KdpA, KdpB, and KdpC. Furthermore, it could be demonstrated that KdpF remains associated with the purified complex. As determined by mass spectrometry, this pe [9789555][21890895] [9789555]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane Membrane Anchored (Experimental)noyesN230.109CYT0-0.200913-10Yn5-16c22/23oCytoplasmicMembrane1029
YAGA_ECOLIP37007yagAb0267 JW0260 cytoplasmic peripheral IM protein, YagACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN290.125CYT0-0.200913-00NiCytoplasmic82
FBPC_ECOLIP37009fbpCafuC yagCb0262 JW0254 cytoplasmic peripheral IM protein, Fe(3+) ions import ATP-binding FbpCHomologous to MalKCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicProphage Integrase (EcoGene)yesnoN620.109CYT0-0.200913-00NiCytoplasmicMembrane742
PT1P_ECOLIP37177ptsPygdF ygdOb2829 JW2797 cytoplasmic peripheral IM protein, Phosphoenolpyruvate-dependent phosphotransferase system PtsPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phosphoenolpyruvate-protein phosphotransferase ptsP Localization to the inner surface of the cytoplasmic membrane by immunoelectron microscopy of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli. [2644649][2644649]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN330.185CYT0-0.200913-00NiCytoplasmic99
PTKB_ECOLIP37188gatBb2093 JW2077 cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIB component GatBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544][16963640] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesY200.743SpI198.62134-00Yn5-14c19/20oCytoplasmic10
DPPF_ECOLIP37313dppFdppEb3540 JW3509 cytoplasmic peripheral IM protein, Dipeptide transport ATP-binding DppFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN530.133CYT0-0.200913-00NiCytoplasmicMembrane924
LHGO_ECOLIP37339lhgOygaFb2660 JW2635 cytoplasmic peripheral IM protein, L-2-hydroxyglutarate oxidase LhgO Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted), Soluble IM FractionCytoplasmicyesyesN210.14CYT0-0.200913-00NiCytoplasmic915
DHAM_ECOLIP37349dhaMycgCb1198 JW5185 cytoplasmic peripheral IM protein, PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN360.134CYT0-0.200913-00NiCytoplasmic974
MENE_ECOLIP37353menEb2260 JW2255 cytoplasmic peripheral IM protein, 2-succinylbenzoate--CoA ligase MenECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homologous to FadD13 protein of Mycobacterium that is peripheral.[22560731]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN480.113CYT0-0.200913-00NiCytoplasmic931
XYLG_ECOLIP37388xylGb3567 JW3539 cytoplasmic peripheral IM protein, Xylose import ATP-binding XylG Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN200.188CYT0-0.200913-00NiCytoplasmicMembrane748
RFAF_ECOLIP37692rfaFwaaFb3620 JW3595 cytoplasmic peripheral IM protein, ADP-heptose--LPS heptosyltransferase 2 RfaFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN420.134CYT0-0.200913-00NiCytoplasmic924
WBBI_ECOLIP37749wbbIyefGb2034 JW2019 cytoplasmic peripheral IM protein, Beta-1,6-galactofuranosyltransferase WbbI WbbICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicnoyesN420.102CYT0-0.200913-00NiCytoplasmic814
WBBJ_ECOLIP37750wbbJyefHb2033 JW2018 cytoplasmic peripheral IM protein, Putative polysaccharide biosynthesis O-acetyl transferase WbbJ Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN240.107CYT0-0.200913-00NiCytoplasmic9
WBBK_ECOLIP37751wbbKyefIb2032 JW2017 cytoplasmic peripheral IM protein, Putative glycosyltransferase WbbK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicnoyesN210.119CYT0-0.200913-00NiUnknown232
RLUB_ECOLIP37765rluByciLb1269 JW1261 cytoplasmic peripheral IM protein, Ribosomal large subunit pseudouridine synthase B RluBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN320.101CYT0-0.200913-00NiCytoplasmic9
YECC_ECOLIP37774yecCb1917 JW1902 cytoplasmic peripheral IM protein, L-cystine transport system ATP-binding YecC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN340.123CYT0-0.200913-00NiCytoplasmicMembrane737
FADK_ECOLIP38135fadKydiDb1701 JW5910 cytoplasmic peripheral IM protein, Medium-chain fatty-acid--CoA ligase FadKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homologous to FadD13 protein of Mycobacterium that is peripheral[22560731]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane; Peripheral membrane protein CytoplasmicnoyesN370.131CYT0-0.200913-00NiCytoplasmic9
NFSB_ECOLIP38489nfsBdprA nfnB nfsI ntrb0578 JW0567 cytoplasmic peripheral IM protein, Oxygen-insensitive NAD(P)H nitroreductase NfsBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [12788749]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cytoplasmic (Experimental)yesyesN180.166CYT0-0.200913-00NiUnknown286
USPG_ECOLIP39177uspGybdQ yzzUb0607 JW0600 cytoplasmic peripheral IM protein, Universal stress UP12 UspGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [8740179] [9600841] [12071968] [16460009] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [8740179] [9600841] [12071968] [16460009] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmic (Experimental)yesyesN530.335CYT0-0.200913-00NiCytoplasmic885
RSGA_ECOLIP39286rsgAengC yjeQb4161 JW4122 ribosomal cytoplasmic peripheral IM protein, Small ribosomal subunit biogenesis GTPase RsgA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)Assembly cofactor of ribosome; associate with the 30S subunit.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomalA, F1, rN/ACytoplasm CytoplasmicribosomeyesyesN530.108CYT0-0.200913-00NiCytoplasmic10
YJFM_ECOLIP39295yjfMb4185 JW4143 cytoplasmic peripheral IM protein, YjfMCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC.[20545840]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicnoyesN550.107CYT0-0.200913-00NiUnknown2105
YJGR_ECOLIP39342yjgRb4263 JW4220 cytoplasmic peripheral IM protein, YjgRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN580.139CYT0-0.200913-00NiUnknown287
FHUF_ECOLIP39405fhuFyjjSb4367 JW4331 cytoplasmic peripheral IM protein, Ferric iron reductase FhuF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)It has a large transmembrane helix in the middle.[14756576]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cytoplasm Cell membrane; Peripheral membrane proteinCytoplasmicyesyesN550.117CYT0-0.200913-01Ni91-112oCytoplasmic1029
RSMC_ECOLIP39406rsmCyjjTb4371 JW4333 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase C RsmCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Ribosom related[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN270.158CYT0-0.200913-00NiCytoplasmic943
ADHP_ECOLIP39451adhPyddNb1478 JW1474 cytoplasmic peripheral IM protein, Alcohol dehydrogenase, propanol-preferring AdhPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN400.124CYT0-0.200913-00NiCytoplasmic986
MTOX_ECOLIP40874solAb1059 JW1046 cytoplasmic peripheral IM protein, N-methyl-L-tryptophan oxidase SolACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN160.149CYT0-0.200913-00NiCytoplasmic973
AZOR_ECOLIP41407azoRacpDb1412 JW1409 cytoplasmic peripheral IM protein, FMN-dependent NADH-azoreductase AzoRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN190.135CYT0-0.200913-00Yn4-13c18/19oCytoplasmic9108
UXAA_ECOLIP42604uxaAygjWb3091 JW3062 cytoplasmic peripheral IM protein, Altronate dehydratase UxaACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN680.185CYT0-0.200913-00NiCytoplasmic8113
OBG_ECOLIP42641obgEcgtA obg yhbZb3183 JW3150 cytoplasmic peripheral IM protein, GTPase ObgE/CgtA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Assembly cofactor of ribosome; associates 30S, 50S,16S, and 23S rRNA. [15292126] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [15292126]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN250.112CYT0-0.200913-00NiCytoplasmic893
HRPA_ECOLIP43329hrpAb1413 JW5905 cytoplasmic peripheral IM protein, ATP-dependent RNA helicase HrpACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN520.107CYT0-0.200913-00NiCytoplasmic8
YCAK_ECOLIP43340ycaKb0901 JW0884 cytoplasmic peripheral IM protein, YcaKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicnoyesN230.238CYT0-0.200913-00NiCytoplasmic995
LPXH_ECOLIP43341lpxHybbFb0524 JW0513 cytoplasmic peripheral IM protein, UDP-2,3-diacylglucosamine hydrolase LpxHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phobius weak prediction of a hydrophobic TMIn the middle of sequence/Kyte-Doolittle predicts several hydrophobic patches [20608695]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN330.16CYT0-0.200913-00NiCytoplasmic1020
UUP_ECOLIP43672uupycbH ycbIb0949 JW0932 cytoplasmic peripheral IM protein, ABC transporter ATP-binding UupCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN270.126CYT0-0.200913-00NiCytoplasmic10
YRAR_ECOLIP45469yraRb3152 JW5528 cytoplasmic peripheral IM protein, Putative NAD(P)-binding YraRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN320.195CYT0-0.200913-00NiCytoplasmic912
PROQ_ECOLIP45577proQyebJ yobE yoeCb1831 JW5300 cytoplasmic peripheral IM protein, RNA chaperone Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN270.162CYT0-0.200913-00NiCytoplasmic869
LUXS_ECOLIP45578luxSygaGb2687 JW2662 cytoplasmic peripheral IM protein, S-ribosylhomocysteine lyase LuxSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein [25664544][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFnoyesN190.106CYT0-0.200913-00NiCytoplasmic8110
GSPE_ECOLIP45759gspEyheGb3326 JW3288 cytoplasmic peripheral IM protein, Putative type II secretion system E GspECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)ATPase of the Type II secretion system (cryptic in K12) EspL (GspL homolog) protein interacts through its N-terminal cytoplasmic domain with EspL (GspL homolog) protein[17255937] [16525507] [PMC398259]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN230.111CYT0-0.200913-00NiCytoplasmic921
YHDZ_ECOLIP45769yhdZb3271 JW3239 cytoplasmic peripheral IM protein, amino-acid ABC transporter ATP-binding YhdZCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN120.122CYT0-0.200913-00NiCytoplasmicMembrane911
YEDJ_ECOLIP46144yedJb1962 JW1945 cytoplasmic peripheral IM protein, YedJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicnoyesN650.139CYT0-0.200913-00NiCytoplasmic8116
YHGF_ECOLIP46837yhgFb3407 JW3370 cytoplasmic peripheral IM protein, YhgFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN240.217CYT0-0.200913-00NiCytoplasmic990
MLC_ECOLIP50456mlcb1594 JW1586 cytoplasmic nucleoid peripheral IM protein, MlcCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)Cytoplasmic global repressor of gene transcription that is recruited to the inner membrane where it binds the PtsB protein after the latter has been phosphorylated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15255904] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1IM (predicted)CytoplasmicyesyesN430.124CYT0-0.200913-00NiCytoplasmic918
AEEP_ECOLIP51981ycjGycjHb1325 JW1318 cytoplasmic peripheral IM protein (SEC through IM), L-Ala-D/L-Glu epimerase YcjGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicyesyesN210.226CYT0-0.200913-00NiCytoplasmic819
CSGD_ECOLIP52106csgDb1040 JW1023 cytoplasmic nucleoid peripheral IM protein, CsgBAC operon transcriptional regulatory protein CsgDCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)Necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. CsgD despite its function as a transcription activator, is localized in the cytoplasmic membrane. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). [20874755] [16513732] [23138451] Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACell inner membrane CytoplasmicyesyesN260.267CYT0-0.200913-00NiCytoplasmic920
YFJP_ECOLIP52131yfjPb2632 JW5419 cytoplasmic peripheral IM protein, YfjPCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Fluorescent fusions of YfjP have been monitored via fluorescence microscopy. YfjP located predominantly beside the membrane.[17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicProphage Integrase (EcoGene)nonoN590.126CYT0-0.200913-00NiUnknown235
FLIJ_ECOLIP52613fliJflaO flaSb1942 JW1926 cytoplasmic peripheral IM protein, FliJ protein FliJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Part of FlgN-FlgK-FliJ-FlhA complex.[22233518]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane; Peripheral membrane protein; Cytoplasmic sideCytoplasmicnoyesN360.121CYT0-0.200913-00NiCytoplasmic1089
WECF_ECOLIP56258wecFrffT yifMb4481 JW5596 cytoplasmic peripheral IM protein, TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase WecFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)Cell inner membrane Cytoplasmicpseudogene (Ochman & Davalos, 2006)yesyesN290.203CYT0-0.200913-00NiUnknown248in/in
SGCB_ECOLIP58035sgcBb4565 JW5967 cytoplasmic peripheral IM protein, Putative phosphotransferase enzyme IIB component SgcB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Part of SgcABC complex, a putative PTSPermease. SgcA (Enzyme IIASgc)Is homologous to the IIA domains of the fructose - and mannitol-specific PTSPermeases (fruB and cmtB) which are peripherally accossiated proteins.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN190.218SpI214.43024-00Yn4-11c19/20oCytoplasmic9
MUKF_ECOLIP60293mukFkicBb0922 JW0905 cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukFCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)[16982609] [20921377] [23230279]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1N/ACytoplasm; nucleoid CytoplasmicyesyesN370.127CYT0-0.200913-00NiCytoplasmic1076
RSMH_ECOLIP60390rsmHmraW yabCb0082 JW0080 cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase H RsmHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [10572301] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [10572301] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN540.11CYT0-0.200913-00NiCytoplasmic1065
HIS1_ECOLIP60757hisGb2019 JW2001 cytoplasmic peripheral IM protein, ATP phosphoribosyltransferase HisGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN440.181CYT0-0.200913-00NiCytoplasmic1064
LEPA_ECOLIP60785lepAb2569 JW2553 cytoplasmic peripheral IM protein, Elongation factor 4 LepACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)LepA has an amino-terminal domain of 112 residues that is homologous to a domain of similar size found in initiation factor 2, elongation factor Tu, and elongation factor G (IF2, EF-Tu, and EF-G). GTP-photoaffinity labeling and subcellular fractionation experiments indicate that LepA a membrane-bound GTP-binding protein [2999765]. GTPase, EF4/LepA involved in translational elongation, EF4 is stored at the membrane of Escherichia coli cells and released into the cytoplasm upon conditions of high ionic strength or low temperature where EF4 binds to stalled ribosomes and remobilizes them[21300907].Trigger factor-sensitive aggregation[19737520]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][2999765] [21300907] [15919657] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicTFyesyesN390.117CYT0-0.200913-00NiCytoplasmicMembrane1045
SYH_ECOLIP60906hisSb2514 JW2498 cytoplasmic peripheral IM protein, Histidine--tRNA ligase HisSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN200.103CYT0-0.200913-00NiCytoplasmic1059
RISB_ECOLIP61714ribEribH ybaFb0415 JW0405 cytoplasmic peripheral IM protein, 6,7-dimethyl-8-ribityllumazine synthase RibECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN690.129CYT0-0.200913-00NiCytoplasmic9
MDH_ECOLIP61889mdhb3236 JW3205 cytoplasmic peripheral IM protein, Malate dehydrogenase MdhCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation. [7028159] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15976458] [7028159] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmic (Experimental)TFyesyesN180.251SpI170.386899-00Yn3-12c17/18oUnknown6
ISPG_ECOLIP62620ispGgcpEb2515 JW2499 cytoplasmic peripheral IM protein, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase IspGCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN440.118CYT0-0.200913-00NiCytoplasmic9
GPMA_ECOLIP62707gpmAgpm pgm pgmAb0755 JW0738 cytoplasmic peripheral IM protein, 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase GpmACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN410.153CYT0-0.200913-00NiCytoplasmic8102
CLPB_ECOLIP63284clpBhtpMb2592 JW2573 cytoplasmic peripheral IM protein, Chaperone ClpBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [8376377]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN220.119CYT0-0.200913-00NiCytoplasmic1075
MLAF_ECOLIP63386mlaFyrbFb3195 JW3162 cytoplasmic peripheral IM protein, Interphospholipid transport system ATP-binding MlaF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN640.109CYT0-0.200913-00NiCytoplasmicMembrane1023
YHES_ECOLIP63389yheSb3352 JW3315 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YheSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding proteins Trigger factor-sensitive aggregation.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN540.115CYT0-0.200913-00NiCytoplasmic939
MLAB_ECOLIP64602mlaByrbBb3191 JW5535 cytoplasmic peripheral IM protein, Interphospholipid transport system binding MlaB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Probable phospholipid ABC transporter-binding protein mlaB Probable phospholipid ABC transporter-binding protein mlaB Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicnoyesN660.108CYT0-0.200913-00NiUnknown2
HLDD_ECOLIP67910hldDhtrM rfaD waaDb3619 JW3594 cytoplasmic peripheral IM protein, ADP-L-glycero-D-manno-heptose-6-epimerase HldDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [2198271]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmic (Experimental)yesyesN190.195CYT0-0.200913-00NiCytoplasmic926
GRCA_ECOLIP68066grcAyfiDb2579 JW2563 cytoplasmic peripheral IM protein, Autonomous glycyl radical cofactor GrcACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN300.139CYT0-0.200913-00NiCytoplasmic9
MALK_ECOLIP68187malKb4035 JW3995 cytoplasmic peripheral IM protein, Maltose/maltodextrin import ATP-binding MalK Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)ATPase of maltose uptake system. MalF functions in maltose transport as a 1:1:2 complex with the integral inner membrane protein MalG and the peripheral inner membrane protein MalK Crystal structure of a catalytic intermediate of the maltose transporter. [18033289][16079137] [18456666]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN150.106CYT0-0.200913-00NiCytoplasmicMembrane1019
SRA_ECOLIP68191srarpsVb1480 JW5962 cytoplasmic peripheral IM protein, Stationary-phase-induced ribosome-associated SraCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Small protein identified experimentally[19121005]. Stationary-phase-induced and ribosome-associated protein[11292794]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry)[23230279].[11292794] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN260.1CYT0-0.200913-00NiCytoplasmic10
FIXC_ECOLIP68644fixCyaaSb0043 JW0042 cytoplasmic peripheral IM protein, FixCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The protein has hydrophobic patches all over it's sequence. Could be part of an electron transfer system required for anaerobic carnitine reduction so this is consistent with a membrane association. Strong Prediction on both Phobius and TMHMM for an aminoterminal weakly hydrophobic region. Other studies propose that this sequence may be an aminoterminal signal peptide without evidence for cleavage. The fixABCX genes in Rhodospirillum Rubrum encode a putative membrane complex participating in electron transfer to nitrogenase where FixA and B are soluble proteins.However the RrFixC expressed in E.coli was localized at the membrane fraction and was not carbonate extracted suggesting that it is an integral membrane protein. From another study in Rhizobia. Primary sequence homology, the FixC family is a highly conserved flavoprotein distantly related to the mitochondrial ETF-QO (peripheral, membrane-associated ETF : ubiquinone oxidoreductase) yet lacking both hydrophobic and amphipathic transmembrane {alpha}-helices. Thus FixC family is unlikely to be stably membrane-associated.[7473063] [15028689] [14702404]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/APeriplasmicyesyesN260.179CYT0-0.200913-00Yn8-19c25/26oCytoplasmic835
AMPA_ECOLIP68767pepAcarP xerBb4260 JW4217 cytoplasmic peripheral IM protein, Cytosol aminopeptidase PepACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN350.112CYT0-0.200913-00NiCytoplasmic958
KAD_ECOLIP69441adkdnaW plsAb0474 JW0463 cytoplasmic peripheral IM protein, Adenylate kinase AdkCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Adk cell localization has been monitored via fluorescence microscopy after protein was chromosomally tagged with fluorescent protein/s. These experiments indicate that Adk is distributed diffusely throughout cytoplasm. [8650159][17272300] [23230279] [8650159]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)yesyesN180.12CYT0-0.200913-00Yn3-12c17/18oCytoplasmic10104
LCFA_ECOLIP69451fadDoldDb1805 JW1794 cytoplasmic peripheral IM protein, Long-chain-fatty-acid--CoA ligase FadDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Acyl-coenzyme A (CoA) synthetase that may interact with the inner membrane. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane CytoplasmicyesyesN630.12CYT0-0.200913-00NiCytoplasmic936
YTFE_ECOLIP69506ytfEb4209 JW4167 cytoplasmic peripheral IM protein, Iron-sulfur cluster repair YtfE Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm CytoplasmicyesyesN210.13CYT0-0.200913-00NiCytoplasmic10103
PTGA_ECOLIP69783crrgsr iex tgs treDb2417 JW2410 cytoplasmic peripheral IM protein, PTS system glucose-specific EIIA component CrrCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12717030] [2960675] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm Cytoplasmic (Experimental)yesyesN440.106CYT0-0.200913-00NiCytoplasmic10113
PTQA_ECOLIP69791chbAcelCb1736 JW1725 cytoplasmic peripheral IM protein, PTS system N,N'-diacetylchitobiose-specific EIIA component ChbACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)IIA component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC.[10913122]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm Cytoplasmicpseudogene (Ochman & Davalos, 2006)yesyesN380.187CYT0-0.200913-00NiCytoplasmic10111
PTQB_ECOLIP69795chbBcelAb1738 JW1727 cytoplasmic peripheral IM protein, PTS system N,N'-diacetylchitobiose-specific EIIB component ChbBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)IIB component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC. [2092358][10913122] [2092358]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm Membrane associated (Experimental)yesyesN280.176CYT0-0.200913-00Yn8-19c27/28oCytoplasmic1097
PTNAB_ECOLIP69797manXgptB ptsLb1817 JW1806 cytoplasmic peripheral IM protein, PTS system mannose-specific EIIAB component ManXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component. Identified by native-PAGE technique for the study of membrane protein complexes in the inner and outer membranes of Escherichia coli. The mannose phosphotransferase system transporter (EIIman) is composed of three subunits, ManX (IIABman), ManY (IICman), and ManZ (IIDman), which are reported to assemble in a 2:1:2 stoichiometry. The IIC and IID components are integral inner membrane proteins. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [10094700] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [15919657] [15911532] [10094700] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)noyesN430.126CYT0-0.200913-00NiCytoplasmic1093
PTFAH_ECOLIP69811fruBfpr fruFb2169 JW2156 cytoplasmic peripheral IM protein, Multiphosphoryl transfer FruBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [8013873] [2841594][15911532] [8013873] [2841594]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasm Cytoplasmic (Experimental)noyesN390.108CYT0-0.200913-00NiCytoplasmic1091
PTFB2_ECOLIP69816frwByijKb3950 JW3922 cytoplasmic peripheral IM protein, PTS system fructose-like EIIB component 2 FrwBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively. SgaT represents a novel prototypical enzyme IIC that functions with SgaA and SgaB to allow phosphoryl transfer from HPr(his-P) to l-ascorbate via the phosphoryl transfer pathwayCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN210.363SpI204.62655-00NiCytoplasmic1087
ULAC_ECOLIP69820ulaCptxA sgaA yjfUb4195 JW4153 cytoplasmic peripheral IM protein, Ascorbate-specific PTS system EIIA component UlaCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)[12644495]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN470.126CYT0-0.200913-00NiCytoplasmicMembrane784
ULAB_ECOLIP69822ulaBsgaB yjfTb4194 JW4152 cytoplasmic peripheral IM protein, Ascorbate-specific PTS system EIIB component UlaBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively[12644495]. All three proteins are required for the anaerobic utilization and uptake of L-ascorbate in vivo and its phosphoenolpyruvate-dependent phosphorylation in vitro[12644495].[12644495]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN620.14CYT0-0.200913-00NiUnknown253
PTMA_ECOLIP69824cmtBb2934 JW2901 cytoplasmic peripheral IM protein, Mannitol-specific cryptic phosphotransferase enzyme IIA component CmtBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)[9525892]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN190.136CYT0-0.200913-00NiUnknown296
PTKA_ECOLIP69828gatAb2094 JW2078/JW2081 cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIA component GatACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Part of phosphoenolpyruvate-dependent carbohydrate transport system (PTS). The carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. GatA (IIA domain) interacts with GatB (IIB domain) [16963640] . Copurifies with trigger factor in pull down assays [19737520].[16963640]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicTFnoyesN690.109CYT0-0.200913-00NiCytoplasmic1071
PTSN_ECOLIP69829ptsNrpoP yhbIb3204 JW3171 cytoplasmic peripheral IM protein, Nitrogen regulatory PtsNCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)enzyme IIA(Ntr) of the nitrogen PTS regulates the intracellular concentration of K(+) by direct interaction with TrkA and KdpD. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17289841] [21605551] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmCytoplasmicyesyesN260.129CYT0-0.200913-00NiCytoplasmic1082
DMSD_ECOLIP69853dmsDynfIb1591 JW5262 cytoplasmic peripheral IM protein, Tat proofreading chaperone DmsD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Chaperone for TAT signal peptides The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. [14522592] DmsD is a redox enzyme maturation protein (REMP) which interacts with the twin-arginine leader sequence of DmsA, the catalytic subunit of DMSO reductase. It interacts with several general chaperones (DnaK, DnaJ, GrpE, GroEL, Tig and Ef-Tu) via in vitro protein-protein interaction screening approaches. It was also found to interact with proteins involved in the molybdenum cofactor biosynthesis pathway. [20153451][19652330] [12813051] [20153451]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN310.453SpI302.43679-00NiCytoplasmicMembrane10
POTA_ECOLIP69874potAb1126 JW1112 cytoplasmic peripheral IM protein, Spermidine/putrescine import ATP-binding PotA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1939142] [7592703] [11976340] [8366082] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane Cytoplasmic (Experimental)yesyesN220.124CYT0-0.200913-00NiCytoplasmicMembrane993
FCTA_ECOLIP69902frcyfdWb2374 JW2371 cytoplasmic peripheral IM protein, Formyl-CoA:oxalate CoA-transferase FrcCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)Hypothesized to interact with the oxalate/formate antiporter and to oxalyl-CoA decarboxylase to form a large complex. 3D structure formyl-CoA transferase in Oxalobacter formigenes reveals a CoA binding site at the interface of an interlocked dimer[12839984].[12839984]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN240.119CYT0-0.200913-00NiCytoplasmic981
DCEB_ECOLIP69910gadBb1493 JW1488 cytoplasmic peripheral IM protein, Glutamate decarboxylase beta GadBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls.[12912902]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN280.112CYT0-0.200913-00NiCytoplasmic1014
HDA_ECOLIP69931hdaidaB yfgEb2496 JW5397 cytoplasmic nucleoid peripheral IM protein, DnaA regulatory inactivator HdaCytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)Homolgous to DnaA and more specific to C-terminal anchor of DnaA.[12618445]Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasmA, N, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN330.157CYT0-0.200913-00NiCytoplasmic9
YAFS_ECOLIP75672yafSb0213 JW0203 cytoplasmic peripheral IM protein, YafSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN540.121CYT0-0.200913-00NiCytoplasmic8
YAHK_ECOLIP75691yahKb0325 JW0317 cytoplasmic peripheral IM protein, Aldehyde reductase YahKCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicnoyesN530.148CYT0-0.200913-00NiCytoplasmic973
GSIA_ECOLIP75796gsiAyliAb0829 JW5897 cytoplasmic peripheral IM protein, Glutathione import ATP-binding GsiA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane CytoplasmicyesyesN620.122CYT0-0.200913-00NiCytoplasmicMembrane9
YBJS_ECOLIP75821ybjSb0868 JW5819 cytoplasmic peripheral IM protein, YbjSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN300.118CYT0-0.200913-00NiCytoplasmic86
YBJX_ECOLIP75829ybjXb0877 JW0861 cytoplasmic peripheral IM protein, YbjXCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)In Shigella the homologous protein is required for the expression, or correct membrane localisation of IcsA (VirG) on the bacterial cell surface.In Pasteurella haemolytica lapB (P32181), which is thought to be membrane-associated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN200.111CYT0-0.200913-00NiUnknown221
LONH_ECOLIP75867ycbZb0955 JW0938 cytoplasmic peripheral IM protein, Putative Lon protease homolog YcbZCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN240.113CYT0-0.200913-00NiCytoplasmic9
RLMI_ECOLIP75876rlmIyccWb0967 JW5898 cytoplasmic peripheral IM protein, Ribosomal RNA large subunit methyltransferase I RlmICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN290.117CYT0-0.200913-00NiCytoplasmic9
YCDY_ECOLIP75915ycdYb1035 JW1018 cytoplasmic peripheral IM protein, Chaperone YcdYCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN380.184CYT0-0.200913-00NiCytoplasmicMembrane989
THIK_ECOLIP75948thiKycfNb1106 JW1092 cytoplasmic peripheral IM protein, Thiamine kinase ThiKCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN390.11CYT0-0.200913-00NiUnknown29
LOLD_ECOLIP75957lolDycfVb1117 JW5162 cytoplasmic peripheral IM protein, -releasing system ATP-binding LolD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN650.122CYT0-0.200913-00NiCytoplasmicMembrane1025
YMFJ_ECOLIP75973ymfJb1144 JW1130 cytoplasmic peripheral IM protein, YmfJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN480.139CYT0-0.200913-00NiCytoplasmic847
YCGR_ECOLIP76010ycgRb1194 JW1183 Flagellar cytoplasmic peripheral IM protein, brake YcgRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Acts as a "backstop brake" to control flagellar motor direction and speed to affect chemotaxis.[20346719] [20303158]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ABacterial flagellum basal body CytoplasmicflagellumnoyesN600.155CYT0-0.200913-00NiCytoplasmic838
OPPD_ECOLIP76027oppDb1246 JW1238 cytoplasmic peripheral IM protein, Oligopeptide transport ATP-binding OppD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Oligopeptide permease (Opp) transporters are located in the plasma membrane and have functions generally related to the uptake of peptides from the extracellular environment. OppD is the ATP-binding protein. [24793159] Based on sequence similarity, OppB and OppC are the membrane components of the ABC transporter, and OppD and OppF are the ATP-binding components of the ABC transporter [1738314] Opp-LacZ hybrid proteins were used to show that OppB, OppC and OppD are membrane-associated proteins. [2821267] [16045610] [22087229 ] [3926486] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][24793159] [1738314] [2821267] [16045610] [22087229] [3926486] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmic (Experimental)yesyesN690.132CYT0-0.200913-00NiCytoplasmicMembrane1018
YCJR_ECOLIP76044ycjRb1314 JW5202 cytoplasmic peripheral IM protein, Probable ketose 3-epimerase YcjRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN470.118CYT0-0.200913-00NiCytoplasmic844
INSH5_ECOLIP76071insH5b1370 JW1363 cytoplasmic peripheral IM protein, Transposase InsH for insertion sequence element IS5Y Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicInsertion Sequence, Transposase, pseudogene (Uniprot)nonoN490.125CYT0-0.200913-00NiCytoplasmic8
PAAC_ECOLIP76079paaCydbPb1390 JW1385 cytoplasmic peripheral IM protein, 1,2-phenylacetyl-CoA epoxidase, subunit C PaaCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicnoyesN570.144CYT0-0.200913-00NiCytoplasmic8
SUFE_ECOLIP76194sufEynhAb1679 JW1669 cytoplasmic peripheral IM protein, Cysteine desulfuration SufECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction .[12941942]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN660.103CYT0-0.200913-00NiCytoplasmic9104
MTFA_ECOLIP76346mtfAyeeIb1976 JW1958 cytoplasmic peripheral IM protein, MtfACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Cytoplasmic transciption factor that shuttles from chromosome to the membranes as a peripheral protein on the PtsG transporter. Specifically it binds to the unphosphorylated, trnasport-mediating form of the EIIBCGlc subunit. [16855233][15063852] [16855233]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN360.109CYT0-0.200913-00NiCytoplasmic887
YEEL_ECOLIP76349yeeLb4497 b1979/b1980cytoplasmic peripheral IM protein, Putative YeeLCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN190.131CYT0-0.200913-00NiCytoplasmic813
ARND_ECOLIP76472arnDpmrJ yfbHb2256 JW2250 cytoplasmic peripheral IM protein, Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN670.132CYT0-0.200913-00NiCytoplasmic910
SIXA_ECOLIP76502sixAyfcWb2340 JW2337 cytoplasmic peripheral IM protein, Phosphohistidine phosphatase SixA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Exhibits phosphohistidine phosphatase activity towards the HPt domain of the ArcB sensor involved in the multistep His-Asp phosphorelay[9489669]. The structure of SixA reveals it forms a relatively shallow groove important for accommodating the kidney-shaped four-helix bundle of the HPt domain of AcrB[17628145].[9489669] [17628145] [10734219]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN570.122CYT0-0.200913-00NiUnknown279
FADI_ECOLIP76503fadIyfcYb2342 JW2339 cytoplasmic peripheral IM protein, 3-ketoacyl-CoA thiolase FadICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN320.192CYT0-0.200913-00NiCytoplasmic99
YFFO_ECOLIP76546yffOb2446 cytoplasmic peripheral IM protein, YffOCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN610.105CYT0-0.200913-00NiCytoplasmic8
YFFR_ECOLIP76549yffRb2449 cytoplasmic peripheral IM protein, YffRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN200.169CYT0-0.200913-00Yn3-14c19/20oUnknown2
MAO2_ECOLIP76558maeBypfFb2463 JW2447 cytoplasmic peripheral IM protein, NADP-dependent malic enzyme MaeBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Trigger factor-sensitive aggregation.[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicTFyesyesN450.165CYT0-0.200913-00NiCytoplasmic981
YGEH_ECOLIP76639ygeHb2852 JW2820 cytoplasmic peripheral IM protein, YgeHCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Fluorescent fusions of YgeH have been monitored via fluorescence microscopy. YgeH located predominantly beside the membrane.[17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN530.108CYT0-0.200913-00NiCytoplasmic819
YNJD_ECOLIP76909ynjDb1756 JW5286 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YnjDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN530.159CYT0-0.200913-00NiCytoplasmicMembrane919
YLBE_ECOLIP77129ylbEb4572 b0519cytoplasmic peripheral IM protein, YlbECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicpseudogene (Ochman & Davalos, 2006)noyesN460.139CYT0-0.200913-00NiCytoplasmic833
YAHF_ECOLIP77187yahFb0320 JW0312 cytoplasmic peripheral IM protein, YahFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN280.139CYT0-0.200913-00NiCytoplasmic8
RSXB_ECOLIP77223rsxBrnfB ydgMb1628 JW1620 cytoplasmic peripheral IM protein, Ion-translocating oxidoreductase complex subunit B RsxBCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Electron transport. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane PeriplasmicyesyesN310.278SpI304.42273-10Yn3-14c19/20oCytoplasmicMembrane1022
LSRA_ECOLIP77257lsrAego ydeXb1513 JW1506 cytoplasmic peripheral IM protein, Autoinducer 2 import ATP-binding LsrACytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicnoyesN420.176CYT0-0.200913-00NiCytoplasmicMembrane1017
DDPD_ECOLIP77268ddpDyddPb1484 JW1479 cytoplasmic peripheral IM protein, Probable D,D-dipeptide transport ATP-binding DdpD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN250.176CYT0-0.200913-00NiCytoplasmicMembrane723
FETA_ECOLIP77279fetAybbLb0490 JW0479 cytoplasmic peripheral IM protein, Probable iron export ATP-binding FetA Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN330.126CYT0-0.200913-00NiCytoplasmicMembrane942
YDJJ_ECOLIP77280ydjJb1774 JW1763 cytoplasmic peripheral IM protein, zinc-type alcohol dehydrogenase-like YdjJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted-detected)CytoplasmicnoyesN460.11CYT0-0.200913-00NiCytoplasmic977
HYFG_ECOLIP77329hyfGb2487 JW2472 cytoplasmic peripheral IM protein, Hydrogenase-4 component G HyfGPhobius predicts a slight hydrophobic area within the body proteinCytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1IM (predicted)CytoplasmicyesyesN270.133CYT0-0.200913-00NiCytoplasmic9
ARNA_ECOLIP77398arnApmrI yfbGb2255 JW2249 cytoplasmic peripheral IM protein, Bifunctional polymyxin resistance ArnACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN590.178CYT0-0.200913-00NiCytoplasmic93
FADJ_ECOLIP77399fadJyfcXb2341 JW2338 cytoplasmic peripheral IM protein, Fatty acid oxidation complex subunit alpha FadJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN700.113CYT0-0.200913-00NiCytoplasmic915
PTFX1_ECOLIP77439fryAypdDb2383 JW2380 cytoplasmic peripheral IM protein, Multiphosphoryl transfer 1 FryACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Multiphosphoryl transfer protein 1 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicnoyesN170.156CYT0-0.200913-00Yn2-10c16/17oCytoplasmic938
SUFS_ECOLIP77444sufScsdB ynhBb1680 JW1670 cytoplasmic peripheral IM protein, Cysteine desulfurase SufSCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS probably through direct interaction.[12941942]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN660.18CYT0-0.200913-00NiCytoplasmic1011
YCJV_ECOLIP77481ycjVymjBb4524 JW1311/JW5203 b1318cytoplasmic peripheral IM protein, Putative ABC transporter ATP-binding YcjV Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicpseudogene (Uniprot)yesnoN370.103CYT0-0.200913-00NiCytoplasmicMembrane910
DXS_ECOLIP77488dxsyajPb0420 JW0410 cytoplasmic peripheral IM protein, 1-deoxy-D-xylulose-5-phosphate synthase DxsCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][23230279] [17272300]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN520.114CYT0-0.200913-00NiCytoplasmic921
PRPE_ECOLIP77495prpEyahUb0335 JW0326 cytoplasmic peripheral IM protein, Propionate--CoA ligase PrpECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to FadD13 protein of Mycobacterium that is peripheral[22560731].[22560731]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicnoyesN440.132CYT0-0.200913-00NiCytoplasmic913
SUFC_ECOLIP77499sufCynhDb1682 JW1672 cytoplasmic peripheral IM protein, Probable ATP-dependent transporter SufC Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction. Homolog to import ATP-binding protein MalK. [12554644][12941942] [12554644]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasm CytoplasmicyesyesN320.213CYT0-0.200913-00NiCytoplasmic1069
YPHE_ECOLIP77509yphEb2547 JW2531 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YphECytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN260.136CYT0-0.200913-00NiCytoplasmicMembrane75
SUFB_ECOLIP77522sufBynhEb1683 JW5273 cytoplasmic peripheral IM protein, FeS cluster assembly SufB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Completely soluble no evidence for hyrophobic patches. IM binding determined by immunolocalization.[11943156]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN310.117CYT0-0.200913-00NiUnknown243
RSXC_ECOLIP77611rsxCrnfC ydgNb1629 JW1621 cytoplasmic peripheral IM protein, Ion-translocating oxidoreductase complex subunit C RsxCCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN540.101CYT0-0.200913-00NiCytoplasmic8
DDPF_ECOLIP77622ddpFyddOb1483 JW1478 cytoplasmic peripheral IM protein, Probable D,D-dipeptide transport ATP-binding DdpF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN320.115CYT0-0.200913-00NiCytoplasmicMembrane918
ARCM_ECOLIP77624yahIb0323 JW0315 cytoplasmic peripheral IM protein, Carbamate kinase-like YahICytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN200.12CYT0-0.200913-00NiCytoplasmic982
SUFA_ECOLIP77667sufAydiCb1684 JW1674 cytoplasmic peripheral IM protein, SufACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)[19366265]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesnoN340.137CYT0-0.200913-00NiCytoplasmic1085
SUFD_ECOLIP77689sufDynhCb1681 JW1671 cytoplasmic peripheral IM protein, FeS cluster assembly SufD Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Forms complex with SufC and SufB.[10322040]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN670.127CYT0-0.200913-00NiCytoplasmic836
OPPF_ECOLIP77737oppFb1247 JW1239 cytoplasmic peripheral IM protein, Oligopeptide transport ATP-binding OppF Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCell inner membrane; Peripheral membrane proteinCytoplasmicyesyesN700.124CYT0-0.200913-00NiCytoplasmicMembrane1017
YDIJ_ECOLIP77748ydiJb1687 JW1677 cytoplasmic peripheral IM protein, YdiJCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279] [15919657]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM FractionCytoplasmicyesyesN290.119CYT0-0.200913-00NiCytoplasmic92
YDCT_ECOLIP77795ydcTb1441 JW1436 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YdcTCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK.Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN200.146CYT0-0.200913-00NiCytoplasmicMembrane1026
YGHU_ECOLIQ46845yghUb2989 JW5492 cytoplasmic peripheral IM protein, Disulfide-bond oxidoreductase YghUCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)Phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. localization peripheral inner membrane protein possibly also integral inner membrane/phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/AMembrane anchoredyesyesN240.332CYT0-0.200913-10NiCytoplasmic949
YQHD_ECOLIQ46856yqhDb3011 JW2978 cytoplasmic peripheral IM protein, Alcohol dehydrogenase YqhDCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)[23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN210.138CYT0-0.200913-00NiCytoplasmic991
CSDA_ECOLIQ46925csdAygdJb2810 JW2781 cytoplasmic peripheral IM protein, Cysteine desulfurase CsdACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)Assembly cofactor of ribosome; associate with the 50S subunit. Under cold-shock conditions CsdA participates as an alternative DEAD-box RNA helicase together with RNase E in a ???cold shock??? RNA degradosome (Prud???homme-Genereux et al., 2004). It is therefore possible that CsdA may associate with the inner membrane via RNAseE.[21529161] [15554978]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicyesyesN590.194CYT0-0.200913-00NiCytoplasmic99
FDRA_ECOLIQ47208fdrAylbDb0518 JW0506 cytoplasmic peripheral IM protein, FdrACytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)[7500942]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell membrane Integral Membrane ProteinyesyesN310.11CYT0-0.200913-00NiUnknown256
TAUB_ECOLIQ47538tauBssiB yaiQb0366 JW0358 cytoplasmic peripheral IM protein, Taurine import ATP-binding TauB Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACell inner membrane CytoplasmicyesyesN570.124CYT0-0.200913-00NiCytoplasmicMembrane1010
YTFR_ECOLIQ6BEX0ytfRytfSb4485 JW5752 cytoplasmic peripheral IM protein, ABC transporter ATP-binding YtfRCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1N/ACytoplasmicpseudogene (Ochman & Davalos, 2006)noyesN250.128CYT0-0.200913-00NiCytoplasmicMembrane1024
ADPP_ECOLIQ93K97nudFaspP yqiE yzzGb3034 JW3002 cytoplasmic peripheral IM protein, ADP-ribose pyrophosphatase NudFCytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)[19766000]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasmA, F1Soluble IM Fraction +CytoplasmicyesyesN380.101CYT0-0.200913-00NiCytoplasmic857