entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocibernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubilitynterm_cterm
SGRT_ECOLIC1P5Z7sgrTb4662 JW0068.1 Putative inhibitor of glucose uptake transporter sgrTPeripheral inner membrane protein facing the cytoplasm (potential).Putative inhibitor of glucose uptake transporter sgrT. SgrT protein expresssion is inducted by α-methylglucoside.[19121005] [19734316] [18042713]Peripheral inner membrane protein facing the cytoplasmF1N/A-noyesN230.105CYT0-0.200913-000iUnknown2
AZUC_ECOLIC1P605azuCb4663 JW1891.1 Uncharacterized protein yhfSPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm.Small protein detected experimentally. AzuC-SPA was most abundant in the inner membrane fraction (77%) indicating this protein is bound to the cytoplasmic side of the inner membrane.[19121005] [21778229]Peripheral inner membrane protein facing the cytoplasmF1N/AMembrane | Peripheral membrane protein-noyesN270.099CYT0-0.200913-000iUnknown2
YSHB_ECOLIC1P620yshBb4686 JW3839.1 Uncharacterized protein yshBPeripheral inner membrane protein facing the cytoplasm.Amphipaseek predicts N-terminal amphipathic helix (1-10). Amphipaseek predicts N-terminal amphipathic helix (1-10). [19121005] Peripheral inner membrane protein facing the cytoplasmF1N/A-noyesN250.194CYT0-0.200913-000iUnknown2
6PGD_ECOLIP00350gndb2029 JW2011 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN200.182CYT0-0.200913-000iCytoplasmic8.9690.25
FRDA_ECOLIP00363frdAb4154 JW4115 Fumarate reductase flavoprotein subunit (EC 1.3.99.1)Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).FrdA and FrdB Fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). [PMC323229] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [PMC323229] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN280.271SpI271.50227-00Yn8-18c27/28oCytoplasmicMembrane9.9950.57
YIDD_ECOLIP0A8C8yidDb4557 JW3682 UPF0161 protein yidDPeripheral inner membrane protein facing the cytoplasm.[21803992]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN420.11CYT0-0.200913-000iUnknown2
XGPT_ECOLIP0A9M5gptgpp gxub0238 JW0228 Xanthine phosphoribosyltransferase (EC 2.4.2.22) (Xanthine-guanine phosphoribosyltransferase) (XGPRT)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN480.21CYT0-0.200913-000iCytoplasmic10
PTHP_ECOLIP0AA04ptsHhprb2415 JW2408 Phosphocarrier protein HPr (EC 2.7.11.-) (Histidine-containing protein)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN220.11CYT0-0.200913-000iCytoplasmic1088.37
DHNA_ECOLIP00393ndhb1109 JW1095 NADH dehydrogenase (EC 1.6.99.3)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Contains amphipathic C-terminal anchor (390-406). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20933494] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell membraneCytoplasmicyesyesN220.114CYT0-0.200913-000iCytoplasmicMembrane1035.8
RIR1_ECOLIP00452nrdAdnaFb2234 JW2228 Ribonucleoside-diphosphate reductase 1 subunit alpha (EC 1.17.4.1) (Protein B1) (Ribonucleoside-diphosphate reductase 1 R1 subunit) (Ribonucleotide reductase 1)Peripheral inner membrane protein facing the cytoplasm (experimental). Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 [9395490] Disulfide bridges between 225 and 462 [9395490] Disulfide bridges between 225 and 462 [9395490][19737520] [23230279] [9395490]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN400.104CYT0-0.200913-000iCytoplasmic9.9763.38
AAT_ECOLIP00509aspCb0928 JW0911 Aspartate aminotransferase (AspAT) (EC 2.6.1.1) (Transaminase A)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic[23230279] [3521591]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmic (Experimental)yesyesN230.126CYT0-0.200913-000iCytoplasmic10
AK1H_ECOLIP00561thrAthrA1 thrA2b0002 JW0001 Bifunctional aspartokinase/homoserine dehydrogenase 1 (Aspartokinase I/homoserine dehydrogenase I) (AKI-HDI) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.3)]Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Cytoplasmic CytoplasmicCopurifies with trigger factor in pull down assays. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [4562989] [354697] [7003595] [6777249][19737520] [15911532] [4562989] [354697] [7003595] [6777249]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmic (Experimental)TFyesyesN150.142CYT0-0.200913-000iCytoplasmic8.96
GLRX3_ECOLIP0AC62grxCyibMb3610 JW3585 Glutaredoxin-3 (Grx3)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bridge (12 15) [23230279] Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN300.112CYT0-0.200913-000iUnknown279.59
AK2H_ECOLIP00562metLmetMb3940 JW3911 Bifunctional aspartokinase/homoserine dehydrogenase 2 (Aspartokinase II/homoserine dehydrogenase II) (AKII-HDII) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.3)]Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN240.117CYT0-0.200913-000iCytoplasmicMembrane9.82
RPOD_ECOLIP00579rpoDaltb3067 JW3039 RNA polymerase sigma factor rpoD (Sigma-70)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[23138451] [19737520] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicTFyesyesN260.104CYT0-0.200913-000iCytoplasmic9.9792.83
DPO1_ECOLIP00582polAresAb3863 JW3835 DNA polymerase I (POL I) (EC 2.7.7.7)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN590.268CYT0-0.200913-000iCytoplasmic9.9776.46
GLRX4_ECOLIP0AC69grxDydhDb1654 JW1646 Glutaredoxin-4 (Grx4) (Monothiol glutaredoxin)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm[23230279] [15833738]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN420.264CYT0-0.200913-000iUnknown290.66
GNSA_ECOLIP0AC92gnsAyccLb4517 JW0976 Protein gnsAPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN500.102CYT0-0.200913-000iUnknown2
ERPA_ECOLIP0ACC3erpAyadRb0156 JW0152 Iron-sulfur cluster insertion protein erpAPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN490.128CYT0-0.200913-000iCytoplasmic9.2684.02
CAPP_ECOLIP00864ppcglub3956 JW3928 Phosphoenolpyruvate carboxylase (PEPC) (PEPCase) (EC 4.1.1.31)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN480.115CYT0-0.200913-000iCytoplasmic8.96
TRPE_ECOLIP00895trpEb1264 JW1256 Anthranilate synthase component 1 (EC 4.1.3.27) (Anthranilate synthase component I)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN190.11CYT0-0.200913-000iCytoplasmic1066.12
TRPG_ECOLIP00904trpDtrpGDb1263 JW1255 Anthranilate synthase component II (EC 4.1.3.27) [Includes: Glutamine amidotransferase; Anthranilate phosphoribosyltransferase (EC 2.4.2.18)]Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic CytoplasmicIdentified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [4594441] [6283099][19766000] [15911532] [4594441] [6283099]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)yesyesN190.166CYT0-0.200913-000iCytoplasmic9.2622.66
HINT_ECOLIP0ACE7hinTycfFb1103 JW1089 HIT-like protein hinTPeripheral inner membrane protein facing the cytoplasm (experimental).[15703176] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN570.182CYT0-0.200913-000iCytoplasmic9.2684.32
YIFE_ECOLIP0ADN2yifEb3764 JW3737 UPF0438 protein yifEPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN440.105CYT0-0.200913-000iUnknown2
ZAPB_ECOLIP0AF36zapByiiUb3928 JW3899 Cell division protein zapBPeripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm[20487275] [18394147]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN190.122CYT0-0.200913-000iUnknown281.78
YGGL_ECOLIP38521yggLb2959 JW2926 Uncharacterized protein yggLPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN290.118CYT0-0.200913-000iCytoplasmic8.96129.09
SYA_ECOLIP00957alaSlovBb2697 JW2667 Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine--tRNA ligase) (AlaRS)Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[23138451] [19737520] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicTFnoyesN260.129CYT0-0.200913-000iCytoplasmic9.9757.55
SYM_ECOLIP00959metGb2114 JW2101 Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA ligase) (MetRS)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation unit. Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN200.115CYT0-0.200913-000iCytoplasmic1049.74
SYGA_ECOLIP00960glyQglyS(A)b3560 JW3531 Glycyl-tRNA synthetase alpha subunit (EC 6.1.1.14) (Glycine--tRNA ligase alpha subunit) (GlyRS)Peripheral inner membrane protein facing the cytoplasm (experimental). Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544][19737520] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFnoyesN570.112CYT0-0.200913-000iCytoplasmic9.9759.14
FHUF_ECOLIP39405fhuFyjjSb4367 JW4331 Ferric iron reductase protein fhuFIt has a large transmembrane helix in the middle.[14756576]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmCell membrane | Peripheral membrane proteinCytoplasmicyesyesN550.117CYT0-0.200913-010i91-112oCytoplasmic1029.18
FLIJ_ECOLIP52613fliJflaO flaSb1942 JW1926 Flagellar fliJ proteinFlagellum.Part of FlgN-FlgK-FliJ-FlhA complex.[22233518] [15546667]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicnoyesN360.121CYT0-0.200913-000iCytoplasmic1089.76
YAEH_ECOLIP62768yaeHb0163 JW0159 UPF0325 protein yaeHPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN260.108CYT0-0.200913-000iCytoplasmic8.9693.3
SYGB_ECOLIP00961glySglyS(B)b3559 JW3530 Glycyl-tRNA synthetase beta subunit (EC 6.1.1.14) (Glycine--tRNA ligase beta subunit) (GlyRS)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation unit.[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN650.169CYT0-0.200913-000iCytoplasmic1077.3
CARB_ECOLIP00968carBpyrAb0033 JW0031 Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase ammonia chain)Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN240.228CYT0-0.200913-00Yn9-16c21/22oUnknown4.99
DNAA_ECOLIP03004dnaAb3702 JW3679 Chromosomal replication initiator protein dnaAPeripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).C-terminal amphiphilic helix (355-370). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10660626] [23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicnoyesN320.146CYT0-0.200913-000iCytoplasmic1038.43
YAAY_ECOLIP75620yaaYb0024 JW5003 Uncharacterized protein yaaYPeripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasmF1N/AMembrane anchorednoyesN430.125CYT0-0.200913-100iCytoplasmicMembrane9.82
YCDY_ECOLIP75915ycdYb1035 JW1018 Uncharacterized protein ycdYPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN380.184CYT0-0.200913-000iCytoplasmicMembrane9.8289.87
THIK_ECOLIP75948thiKycfNb1106 JW1092 Thiamine kinase (EC 2.7.1.89)Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN390.11CYT0-0.200913-000iUnknown29.13
LACI_ECOLIP03023lacIb0345 JW0336 Lactose operon repressorPeripheral inner membrane protein facing the cytoplasm (potential). Nucleoid. Peripheral inner membrane protein facing the cytoplasm (experimental).Regulates lac operon transcription when in homotetrameric form.[21936772] [15863616] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN330.136CYT0-0.200913-000iCytoplasmic1032.29
GUAA_ECOLIP04079guaAb2507 JW2491 GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2) (GMP synthetase) (GMPS) (Glutamine amidotransferase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN450.122CYT0-0.200913-000iCytoplasmic9.97
AMPN_ECOLIP04825pepNb0932 JW0915 Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide hydrolase)Peripheral inner membrane protein facing the cytoplasm or cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Homologous peptidases are membrane associated in mammals, some data propose that the enzyme may associate with the E.coli membrane [323010]. It is structurally classified with EC 3.4.11.2 membrane alanyl aminopeptidases. However, the extent of membrane association needs further analysis as it is generally soluble in the cytoplasmic fraction of E.coli and other bacteria. [323010] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][323010] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmic (Experimental)yesyesN500.108CYT0-0.200913-000iCytoplasmicMembrane10101.76
SUFA_ECOLIP77667sufAydiCb1684 JW1674 Fumarate and nitrate reduction regulatory proteinPeripheral inner membrane protein facing the cytoplasm.[19366265]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesnoN340.137CYT0-0.200913-000iCytoplasmic1085.65
RBSA_ECOLIP04983rbsAb3749 JW3728 Ribose import ATP-binding protein RbsA (EC 3.6.3.17)Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN350.548CYT0-0.200913-000iCytoplasmicMembrane1049.04
FUMC_ECOLIP05042fumCb1611 JW1603 Fumarate hydratase class II (Fumarase C) (EC 4.2.1.2)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN570.12CYT0-0.200913-000iCytoplasmic1064.99
PNP_ECOLIP05055pnpb3164 JW5851 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) (Polynucleotide phosphorylase) (PNPase)Peripheral inner membrane protein (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm.Polynucleotide phosphorylase (PNPase) or Polyribonucleotide nucleotidyltransferase Is a cytoplasmic enzyme that has also been isolated in association with the inner membrane (Stenberg et al., 2005; Maddalo et al., 2011). It is not known if association is direct. The protein has weak hydrophobic patches and three regions with weak predictions from Phobius for membrane interaction. Membrane association may be via binding to RNAaseE with which PNPase interacts as part of the multienzyme complex known as the RNA degradosome. RNAseE has been shown to bind to membranes (Khemici et al., 2008). The interaction of RNase E with PNPase , brings together two enzymes important in the degradation of mRNA. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][18976283] [16079137] [21210718] [19737520] [23230279] [24438330]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicTFyesyesN300.323CYT0-0.200913-000iCytoplasmic1074.02
PTHA_ECOLIP05706srlBgutBb2704 JW2673 Glucitol/sorbitol-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (EIIA-Gut) (EIII-Gut) (PTS system glucitol/sorbitol-specific EIIA component)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).PTS system glucitol/sorbitol-specific EIIA component.The hydropathic profile of the Enzyme IIgut revealed 7 or 8 long hydrophobic segments which may traverse the cell membrane as alpha-helices as well as 2 or 4 short strongly hydrophobic stretches which may traverse the membrane as beta-structure [3553176].[2824435] [3553176]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN350.143CYT0-0.200913-000iCytoplasmic8.96115.95
ILVD_ECOLIP05791ilvDb3771 JW5605 Dihydroxy-acid dehydratase (DAD) (EC 4.2.1.9)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN540.166CYT0-0.200913-000iCytoplasmic9.9725.68
ILVC_ECOLIP05793ilvCb3774 JW3747 Ketol-acid reductoisomerase (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (Alpha-keto-beta-hydroxylacil reductoisomerase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN290.107CYT0-0.200913-000iCytoplasmic1097.29
BGLR_ECOLIP05804uidAgurA gusAb1617 JW1609 Beta-glucuronidase (GUS) (EC 3.2.1.31) (Beta-D-glucuronoside glucuronosohydrolase)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN590.12CYT0-0.200913-000iCytoplasmic8.9646.03
DLD_ECOLIP06149dldb2133 JW2121 D-lactate dehydrogenase (EC 1.1.1.28) (Respiratory D-lactate dehydrogenase)Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Requires phospholipid for maximal activity. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10944213] [22108384] [15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicYidCyesyesN330.121CYT0-0.200913-000iCytoplasmicMembrane1078.9
BTUD_ECOLIP06611btuDb1709 JW1699 Vitamin B12 import ATP-binding protein BtuD (EC 3.6.3.33) (Vitamin B12-transporting ATPase)Vitamin B12 import ATP-binding protein btuD.Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN260.195CYT0-0.200913-000iCytoplasmicMembrane109.27
ERA_ECOLIP06616erarbaA sdgEb2566 JW2550 GTP-binding protein eraPeripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmAssembly cofactor of ribosome; associate with the 30S subunit and 16S rRNA. YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][21529161] [8282709] [17272300]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinCytoplasmicyesyesN410.127CYT0-0.200913-000iCytoplasmicMembrane1092.38
ODP2_ECOLIP06959aceFb0115 JW0111 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (E2)Inner membrane protein (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15911532] [2699406] [19737520] [24742683] [15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFnoyesN520.106CYT0-0.200913-000iCytoplasmic1095.06
FLIM_ECOLIP06974fliMcheC2 fla AII fla QIIb1945 JW1929 Flagellar motor switch protein FliMFlaggelar C-ring proteins - FliG, FliM, and FliN.[15546667]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane proteinBacterial flagellum basal bodyCytoplasmicflagellumnoyesN100.109CYT0-0.200913-000iCytoplasmicMembrane1057.89
HIS7_ECOLIP06987hisBb2022 JW2004 Histidine biosynthesis bifunctional protein hisB [Includes: Histidinol-phosphatase (EC 3.1.3.15); Imidazoleglycerol-phosphate dehydratase (IGPD) (EC 4.2.1.19)]Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN240.105CYT0-0.200913-000iCytoplasmic1066.51
RSMA_ECOLIP06992rsmAksgAb0051 JW0050 Ribosomal RNA small subunit methyltransferase A (EC 2.1.1.-) (16S rRNA dimethyladenosine transferase) (16S rRNA dimethylase) (High level kasugamycin resistance protein ksgA) (Kasugamycin dimethyltransferase) (S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Assembly cofactor of ribosome; Overexpression of RsmA suppresses defects in mutant of era. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN330.128CYT0-0.200913-000iCytoplasmic10
MALT_ECOLIP06993malTmalAb3418 JW3381 HTH-type transcriptional regulator MalTPeripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid protein (experimental). NucleoidCytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the MalK peripheral ATPase of the maltose transporter. Trigger factor-sensitive aggregation. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). [15063852] [23138451] [19737520] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicTFyesyesN360.12CYT0-0.200913-000iUnknown7.338.98
PLDB_ECOLIP07000pldBb3825 JW5584 Lysophospholipase L2 (EC 3.1.1.5) (Lecithinase B)Peripheral inner membrane protein facing the cytoplasm (experimental).Gene and characteristics of deduced amino acid sequence of lysophospholipase L2. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3908445] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN340.104CYT0-0.200913-000iCytoplasmicMembrane7.8836.47
POXB_ECOLIP07003poxBb0871 JW0855 Pyruvate dehydrogenase [ubiquinone] (EC 1.2.5.1) (Pyruvate oxidase) (POX) [Cleaved into: Alpha-peptide]Peripheral inner membrane protein facing the cytoplasm (potential).Is one of several membrane-associated form of the enzyme has a tendency to self-aggregate,is flavo protein dehydrogenases that feed electrons in to the high lysurfacelabile,and manifests. Pyruvate oxidase is a membrane-associated flavoprotein dehydrogenase which is greatly activated by lipids and detergents. The carboxyl-terminal region of the protein has been shown to play a critical role in the interaction with lipids.[18988747] [2663858]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinCytoplasmicyesyesN620.106CYT0-0.200913-000iCytoplasmicMembrane1020.44
PROA_ECOLIP07004proAb0243 JW0233 Gamma-glutamyl phosphate reductase (GPR) (EC 1.2.1.41) (Glutamate-5-semialdehyde dehydrogenase) (Glutamyl-gamma-semialdehyde dehydrogenase) (GSA dehydrogenase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN480.168CYT0-0.200913-000iCytoplasmic10118.52
DHSB_ECOLIP07014sdhBb0724 JW0714 Succinate dehydrogenase iron-sulfur subunit (EC 1.3.99.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [6388571] [2987185] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [21541338] [6388571] [2987185] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN280.102CYT0-0.200913-000iCytoplasmicMembrane1034.19
HISP_ECOLIP07109hisPb2306 JW2303 Histidine transport ATP-binding protein hisPPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN310.431CYT0-0.200913-000iCytoplasmicMembrane1067.39
CHEA_ECOLIP07363cheAb1888 JW1877 Chemotaxis protein CheA (EC 2.7.13.3)Peripheral inner membrane protein facing the cytoplasm (potential).[19766000]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted), Soluble IM Fraction +CytoplasmCytoplasmicnoyesN170.108CYT0-0.200913-000iCytoplasmic1088.79
SYFB_ECOLIP07395pheTb1713 JW1703 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) (Phenylalanine--tRNA ligase beta chain) (PheRS)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN290.131CYT0-0.200913-000iCytoplasmic9.9779.83
TYPH_ECOLIP07650deoAtpp ttgb4382 JW4345 Thymidine phosphorylase (EC 2.4.2.4) (TdRPase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicnoyesN530.104CYT0-0.200913-000iCytoplasmic8.9612.62
FHUC_ECOLIP07821fhuCb0151 JW0147 Iron(3+)-hydroxamate import ATP-binding protein fhuC (EC 3.6.3.34) (Ferric hydroxamate uptake protein C) (Ferrichrome transport ATP-binding protein fhuC) (Iron(III)-hydroxamate import ATP-binding protein fhuC)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1551849] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN390.111CYT0-0.200913-000iCytoplasmicMembrane1045.5
IDH_ECOLIP08200icdicdA icdEb1136 JW1122 Isocitrate dehydrogenase [NADP] (IDH) (EC 1.1.1.42) (IDP) (NADP(+)-specific ICDH) (Oxalosuccinate decarboxylase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicTFyesyesN470.119CYT0-0.200913-000iCytoplasmic9.9796.01
SYFA_ECOLIP08312pheSb1714 JW5277 Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20) (Phenylalanine--tRNA ligase alpha chain) (PheRS)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN190.166CYT0-0.200913-000iCytoplasmic1012.33
DNAJ_ECOLIP08622dnaJgroPb0015 JW0014 Chaperone protein dnaJ (HSP40) (Heat shock protein J)Peripheral inner membrane protein facing the cytoplasm (experimental). Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [20153451]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN390.105CYT0-0.200913-000iCytoplasmic1052.19
PT1_ECOLIP08839ptsIb2416 JW2409 Phosphoenolpyruvate-protein phosphotransferase (EC 2.7.3.9) (Phosphotransferase system, enzyme I)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental). [2644649] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN150.115CYT0-0.200913-000iCytoplasmic1090.19
NARG_ECOLIP09152narGbisD narCb1224 JW1215 Respiratory nitrate reductase 1 alpha chain (EC 1.7.99.4) (Nitrate reductase A subunit alpha) (Quinol-nitrate oxidoreductase subunit alpha)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. [12910261] In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. [19295650] When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. [2674654] The heterotrimeric NarGHI has an αβγ structure encoded by narGHI respectively. The α and β subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane γ subunit. [3371362] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe–S] clusters and molybdenum in a sequential manner. Insertion of the [Fe–S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12910261] [19295650] [2674654] [3371362] [16286471] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membrane | Peripheral membrane proteinCytoplasmicyesyesN200.124CYT0-0.200913-000iCytoplasmicMembrane1029.62
GRPE_ECOLIP09372grpEb2614 JW2594 Protein grpE (HSP-70 cofactor) (HSP24) (Heat shock protein B25.3)Peripheral inner membrane protein facing the cytoplasm (experimental). Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [20153451]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN350.101CYT0-0.200913-000iCytoplasmic9.97
PFLB_ECOLIP09373pflBpflb0903 JW0886 Formate acetyltransferase 1 (EC 2.3.1.54) (Pyruvate formate-lyase 1)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicTFyesyesN480.103CYT0-0.200913-000iCytoplasmic1030.58
PUTA_ECOLIP09546putApoaAb1014 JW0999 Bifunctional protein putA [Includes: Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase); Delta-1-pyrroline-5-carboxylate dehydrogenase (P5C dehydrogenase) (EC 1.5.1.12)]Peripheral inner membrane protein facing the cytoplasm. Nucleoid protein (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).The proline dehydrogenase PutA protein from S. typhimurium was shown to act as a membrane-associated enzyme or as a transcriptional repressor [8483946]. Catabolism of proline requires PutA to associate with the membrane because reoxidation of the FAD cofactor in PutA needs the presence of an electron acceptor. Repression of the put operon requires PutA to bind to the put control-region DNA in the cytoplasm. Transcription factor [23138451]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][8483946] [23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1Soluble IM FractionCytoplasmicyesyesN640.118CYT0-0.200913-000iCytoplasmic9.97
GLTB_ECOLIP09831gltBaspBb3212 JW3179 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) (Glutamate synthase subunit alpha) (GLTS alpha chain) (NADPH-GOGAT)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN480.135CYT0-0.200913-000iCytoplasmic9.97
MODC_ECOLIP09833modCchlD narDb0765 JW0748 Uncharacterized protein ybbVPeripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN350.128CYT0-0.200913-000iCytoplasmicMembrane10
UVRA_ECOLIP0A698uvrAdinEb4058 JW4019 UvrABC system protein A (UvrA protein) (Excinuclease ABC subunit A)Peripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [19737520] Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicTFnoyesN420.254CYT0-0.200913-000iCytoplasmic9.97
ACKA_ECOLIP0A6A3ackAackb2296 JW2293 Acetate kinase (EC 2.7.2.1) (Acetokinase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN200.328CYT0-0.200913-000iCytoplasmic10112.72
ACP_ECOLIP0A6A8acpPb1094 JW1080 Acyl carrier protein (ACP) (Cytosolic-activating factor) (CAF) (Fatty acid synthase acyl carrier protein)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Part of phospholipid metabolism complex PlsB-PssA-YbgC-Acp[16294310]. Various methods were used to demonstrate that ACP could be cross-linked specifically to an inner membrane protein of E. coli, most probably to the glycerol-3-phosphate acyl transferase (GPAT)[1310877]. Evidence for interactions of acyl carrier protein with glycerol-3-phosphate acyltransferase, an inner membrane protein of Escherichia coli[2676605]. Membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein[2668033]. [3884600] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16294310] [1310877] [2676605] [2668033] [3884600] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmic (Experimental)pseudogene (Ochman & Davalos, 2006)noyesN470.119CYT0-0.200913-000iCytoplasmic10
ISCS_ECOLIP0A6B7iscSyfhO yzzOb2530 JW2514 Cysteine desulfurase (EC 2.8.1.7) (NifS protein homolog) (ThiI transpersulfidase)Peripheral inner membrane protein facing the cytoplasm (experimental).IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled.[10891064] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN480.104CYT0-0.200913-000iCytoplasmic9.9737.46
ARGB_ECOLIP0A6C8argBb3959 JW5553 Acetylglutamate kinase (EC 2.7.2.8) (N-acetyl-L-glutamate 5-phosphotransferase) (NAG kinase) (AGK)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN220.115CYT0-0.200913-000iCytoplasmic9.2681.14
ASSY_ECOLIP0A6E4argGb3172 JW3140 Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN370.163CYT0-0.200913-000iCytoplasmic9.9719.39
ATPE_ECOLIP0A6E6atpCpapG uncCb3731 JW3709 ATP synthase epsilon chain (ATP synthase F1 sector epsilon subunit) (F-ATPase epsilon subunit)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [21778229] [9868784] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)noyesN410.107CYT0-0.200913-000iCytoplasmicMembrane10120.16
CARA_ECOLIP0A6F1carApyrAb0032 JW0030 Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase glutamine chain)Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN220.124CYT0-0.200913-000iCytoplasmic9.9760.46
GLPK_ECOLIP0A6F3glpKb3926 JW3897 Glycerol kinase (EC 2.7.1.30) (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN220.143CYT0-0.200913-000iCytoplasmic9.9721.86
CH60_ECOLIP0A6F5groLgroEL mopAb4143 JW4103 60 kDa chaperonin (Protein Cpn60) (groEL protein)Peripheral inner membrane protein facing the cytoplasm (experimental). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338][23230279] [21541338]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN580.12CYT0-0.200913-000iCytoplasmic1094.55
CH10_ECOLIP0A6F9groSgroES mopBb4142 JW4102 10 kDa chaperonin (Protein Cpn10) (groES protein)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [22380631] [20153451]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN430.185CYT0-0.200913-000iCytoplasmic1086.32
HSLU_ECOLIP0A6H5hslUhtpIb3931 JW3902 ATP-dependent protease ATPase subunit HslU (Heat shock protein HslU) (Unfoldase HslU)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN240.141CYT0-0.200913-000iCytoplasmic1074.44
DADA_ECOLIP0A6J5dadAdadRb1189 JW1178 D-amino acid dehydrogenase small subunit (EC 1.4.99.1)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][6102989] [19737520] [22108384] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicTFYidCyesyesN230.155TMH00.264511-00Yn3-11c16/17oCytoplasmicMembrane1012.21
DEF_ECOLIP0A6K3deffmsb3287 JW3248 Peptide deformylase (PDF) (EC 3.5.1.88) (Polypeptide deformylase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN420.113CYT0-0.200913-000iCytoplasmic9.9766.82
DEOB_ECOLIP0A6K6deoBdrm thyRb4383 JW4346 Phosphopentomutase (EC 5.4.2.7) (Phosphodeoxyribomutase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN400.147CYT0-0.200913-000iCytoplasmic10101.22
DEOC_ECOLIP0A6L0deoCdra thyRb4381 JW4344 Deoxyribose-phosphate aldolase (EC 4.1.2.4) (Phosphodeoxyriboaldolase) (DERA) (Deoxyriboaldolase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN220.136CYT0-0.200913-000iCytoplasmic10115.23
EFG_ECOLIP0A6M8fusAfar fusb3340 JW3302 Elongation factor G (EF-G)Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmicTranslation [7042386][23230279] [7042386]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmic (Experimental)yesyesN390.11CYT0-0.200913-000iCytoplasmic9.9762.49
EFTS_ECOLIP0A6P1tsfb0170 JW0165 Elongation factor Ts (EF-Ts)Peripheral inner membrane protein facing the cytoplasm (experimental). Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338][23230279] [21541338]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN590.109CYT0-0.200913-000iCytoplasmic1087.2
DER_ECOLIP0A6P5derengA yfgKb2511 JW5403 GTP-binding protein engA (Double era-like domain protein)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Essential protein, proposed to be engaged in a functional cyclic interaction between ribosomes and the membrane in Escherichia coli through interaction with the 50S subunit. Assembly cofactor of ribosome; associates with the 50S subunit and YihI. YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11387344] [20172997] [16930151] [21354313] [21529161] [17272300] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN310.139CYT0-0.200913-000iCytoplasmic9.9766
ENGB_ECOLIP0A6P7engByihAb3865 JW5930 Probable GTP-binding protein engBCytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Fluorescent fusions of YqiK have been monitored via fluorescence microscopy. YqiK was found to be distributed diffusely throughout cytoplasm.[17272300] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN430.126CYT0-0.200913-000iUnknown2
ENO_ECOLIP0A6P9enob2779 JW2750 Enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)Peripheral inner membrane protein facing the cytoplasm (potential). Cytoplasmic Excreted. Cytoskeleton. Cytoplasmic Cytoplasmic Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. [8610017] [9600841] [9740056] [9732527] [12035760] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17242352] [8610017] [15003462] [18337249] [9600841] [9740056] [9732527] [12035760] [23230279]Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular spaceA, F1, F4Soluble IM FractionCytoplasm -> cytoskeleton | Secreted | Cell surfaceCytoplasmic (Experimental)Uknown mechanismyesyesN420.139CYT0-0.200913-000iCytoplasmic9.97100.97
FABZ_ECOLIP0A6Q6fabZsefA yaeAb0180 JW0175 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase ((3R)-hydroxymyristoyl ACP dehydrase) (EC 4.2.1.-) (17 kDa actomyosin component)Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Cytoplasmic Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. [8354462] [7806516] [8805534] [8910376] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15911532] [8910376] [19737520] [8354462] [7806516] [8805534] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmic (Experimental)TFyesyesN320.104CYT0-0.200913-000iCytoplasmic1027.87
FIS_ECOLIP0A6R3fisb3261 JW3229 DNA-binding protein fis (Factor-for-inversion stimulation protein) (Hin recombinational enhancer-binding protein)Nucleoid-associated protein (NAP) (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Nucleoid-associated protein (NAP).[23138451] [21541338] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN190.116CYT0-0.200913-000iCytoplasmic9.26110.24
MNMG_ECOLIP0A6U3mnmGgidA trmFb3741 JW3719 tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG (Glucose-inhibited division protein A)Cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental)Similar localization to mnmE; may form a complex together [17062623]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN250.172CYT0-0.200913-010o601-619iCytoplasmic9.976.87
GLGA_ECOLIP0A6U8glgAb3429 JW3392 Glycogen synthase (EC 2.4.1.21) (Starch [bacterial glycogen] synthase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN320.162CYT0-0.200913-000iCytoplasmic9.9710.71
HEM1_ECOLIP0A6X1hemAb1210 JW1201 Glutamyl-tRNA reductase (GluTR) (EC 1.2.1.70)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation unit[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN440.116CYT0-0.200913-000iCytoplasmic9.9723.41
HFQ_ECOLIP0A6X3hfqb4172 JW4130 Protein hfq (HF-1) (Host factor-I protein) (HF-I)Peripheral inner membrane protein (experimental). Nucleoid-associated protein (NAP) (experimental). Peripheral inner membrane protein facing the cytoplasm.The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain. Is a bacterial protein involved in several aspects of nucleic acid transactions. Nucleoid-associated protein (NAP). Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][20011543] [21541338] [24438330]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN530.107CYT0-0.200913-000iCytoplasmic1075.65
IHFA_ECOLIP0A6X7ihfAhid himAb1712 JW1702 Integration host factor subunit alpha (IHF-alpha)Nucleoid Peripheral inner membrane protein facing the cytoplasm (experimental). NucleoidTranslation. Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature [21541338] [23230279] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicnoyesN240.109CYT0-0.200913-000iCytoplasmic9.97
IHFB_ECOLIP0A6Y1ihfBhimD hipb0912 JW0895 Integration host factor subunit beta (IHF-beta)Nucleoid Peripheral inner membrane protein facing the cytoplasm (experimental). NucleoidTranslation, nucleoid-associated protein (NAP). Bacterial histone-like DNA-binding proteins signature [21541338] [23230279] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicnoyesN390.116CYT0-0.200913-000iCytoplasmic9.9723.23
HSLO_ECOLIP0A6Y5hslOyrfIb3401 JW5692 33 kDa chaperonin (Heat shock protein 33) (HSP33)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bridge (230 232;263 266) [10764757][23230279] [10764757]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmCytoplasmicyesyesN630.125CYT0-0.200913-000iCytoplasmic1088.87
DNAK_ECOLIP0A6Y8dnaKgroP grpF segb0014 JW0013 Chaperone protein dnaK (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). DnaK associates with the inner membrane. The ability to isolate DnaK with a membrane fraction prepared under gentle lysis conditions supports a peripheral association between DnaK and the cytoplasmic membrane. [7961473] Overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to green fluorescent protein (GFP) led to an increased association (2.3–4.8-fold) of DnaK with the membrane. [17446557] [1396676] [7783627] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][7961473] [16079137] [7961473] [17446557] [1396676] [7783627] [23230279] [20153451]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN200.127CYT0-0.200913-000iCytoplasmic10115.64
HTPG_ECOLIP0A6Z3htpGb0473 JW0462 Chaperone protein htpG (Heat shock protein C62.5) (Heat shock protein htpG) (High temperature protein G)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN430.189CYT0-0.200913-000iCytoplasmic10109.49
IF2_ECOLIP0A705infBssyGb3168 JW3137 Translation initiation factor IF-2Peripheral inner membrane protein facing the cytoplasm (experimental).Translation. Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN280.114CYT0-0.200913-000iCytoplasmic9.9765
IF3_ECOLIP0A707infCfitb1718 JW5829 Translation initiation factor IF-3 [Cleaved into: Translation initiation factor IF-3, N-terminally processed; Translation initiation factor IF-3S]Peripheral inner membrane protein facing the cytoplasm (experimental).Translation. Trigger factor-sensitive aggregation. Evidence that the inter-domain region is disordered in solution.[19737520] [9054966] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFnoyesN220.117CYT0-0.200913-000iCytoplasmic10113.58
KPRS_ECOLIP0A717prsprsAb1207 JW1198 Ribose-phosphate pyrophosphokinase (RPPK) (EC 2.7.6.1) (Phosphoribosyl pyrophosphate synthase) (P-Rib-PP synthase) (PRPP synthase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN310.131CYT0-0.200913-000iCytoplasmic109.48
MINE_ECOLIP0A734minEb1174 JW1163 Cell division topological specificity factorPeripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm.MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature. [21738659][20025670] [10096083] [21738659]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN180.123CYT0-0.200913-000iCytoplasmic9.97101.97
KEFF_ECOLIP0A754kefFyabFb0046 JW0045 Glutathione-regulated potassium-efflux system ancillary protein kefFPeripheral inner membrane protein facing the cytoplasm.Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from Escherichia coli, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity.[17679694]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN170.127CYT0-0.200913-000iCytoplasmic9.9734.3
KEFG_ECOLIP0A756kefGyheRb3351 JW3314 Glutathione-regulated potassium-efflux system ancillary protein kefGPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from Escherichia coli, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17679694] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN230.13CYT0-0.200913-000iCytoplasmic9.26
NAGB_ECOLIP0A759nagBglmDb0678 JW0664 Glucosamine-6-phosphate deaminase (EC 3.5.99.6) (GlcN6P deaminase) (GNPDA) (Glucosamine-6-phosphate isomerase)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bridge (219 219) [8240271][23230279] [8240271]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN320.107CYT0-0.200913-000iCytoplasmic8.9628.22
NDK_ECOLIP0A763ndkb2518 JW2502 Nucleoside diphosphate kinase (NDK) (NDP kinase) (EC 2.7.4.6) (Nucleoside-2-P kinase)Peripheral Inner membrane protein facing the cytoplasm (potential). Peripheral Inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (experimental).Nucleoside diphosphate kinase (EC 2.7.4.6) (Ndk) is a ubiquitous enzyme functioning in the intracellular distribution of terminal phosphate bond energy among the various nucleotides used in synthetic and regulatory functions in cells[8606147]. Based on studies in Pseudomonas and many other systems including mitochondria, the uracil-processing DNA repair nuclease ndk is known to interact to plasma membranes and mitochondrial inner membrane[15096615]. Binding is regulated by nucleotides[19146889]. In Pseudomonas, an intracellular protease cleaves the 16-kDa form of Ndk to a 12-kDa form that undergoes autophosphorylation with an efficiency almost identical to that of the 16-kDa form. The 12-kDa form is predominantly associated with the cell membrane fraction, whereas the 16-kDa form is predominantly cytoplasmic. In the membrane-associated state, the 12-kDa form of Ndk was found to synthesize GTP in preference to other nucleoside triphosphates. The formation of the 12-kDa form of Ndk and its association with the cell membrane were found to be related to the growth stage of P.aeruginosa, with less than 1% of the 12-kDa Ndk detectable in the membrane fraction at early log phase in comparison with the levels present at late stationary phase[8606147]. Cytosolic Ndks bind directly to membrane lipids in a dynamic process and can be fully reconstituted with phospholipids and recombinant Ndk [19146889]. Ndks may act as a reservoir of high energy phosphate, supporting the operation of membrane-based processes that utilize nucleotides other than ATP, such as intracellular traffic and phospholipid biosynthesis. Trigger factor-sensitive aggregation[19737520]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19146889] [15096615] [19737520] [8606147] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFnoyesN170.158CYT0-0.200913-000iCytoplasmic1064.67
RPPH_ECOLIP0A776rppHnudH ygdPb2830 JW2798 RNA pyrophosphohydrolase (EC 3.6.1.-) ((Di)nucleoside polyphosphate hydrolase) (Ap5A pyrophosphatase)Peripheral inner membrane protein facing the cytoplasm. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN250.126CYT0-0.200913-000iCytoplasmic9.2662.91
K6PF1_ECOLIP0A796pfkAb3916 JW3887 6-phosphofructokinase isozyme 1 (EC 2.7.1.11) (6-phosphofructokinase isozyme I) (Phosphofructokinase 1) (Phosphohexokinase 1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN200.426CYT0-0.200913-000iCytoplasmic1076.65
PGK_ECOLIP0A799pgkb2926 JW2893 Phosphoglycerate kinase (EC 2.7.2.3)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN210.107CYT0-0.200913-000iCytoplasmic10100.13
PLSB_ECOLIP0A7A7plsBb4041 JW4001 Glycerol-3-phosphate acyltransferase (GPAT) (EC 2.3.1.15)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Phospholipid metabolism complex PlsB-PssA-YbgC-acp Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16294310] [4943977] [15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicnoyesN270.194CYT0-0.200913-000iCytoplasmicMembrane10
PPK_ECOLIP0A7B1ppkb2501 JW2486 Polyphosphate kinase (EC 2.7.4.1) (ATP-polyphosphate phosphotransferase) (Polyphosphoric acid kinase)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1331061] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN260.112CYT0-0.200913-000iCytoplasmicMembrane107.84
NADK_ECOLIP0A7B3ppnKyfjB yfjEb2615 JW2596 Probable inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase) (EC 2.7.1.23)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasmFluorescent fusions of ppnK have been monitored via fluorescence microscopy. ppnK distributed irregularly throughout the cytoplasm, with some clusters formed. NadK has been viewed through fluorescence microscopy and is associated with the membrane, in all or a proportion of the cells analyzed. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][17272300] [17272300]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN200.111CYT0-0.200913-000iCytoplasmic9.9732.41
PURA_ECOLIP0A7D4purAadeKb4177 JW4135 Adenylosuccinate synthetase (AMPSase) (AdSS) (EC 6.3.4.4) (IMP--aspartate ligase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN620.102CYT0-0.200913-000iCytoplasmic1041.37
PUR7_ECOLIP0A7D7purCb2476 JW2461 Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6) (SAICAR synthetase)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN650.127CYT0-0.200913-000iCytoplasmic9.9750.3
PYRD_ECOLIP0A7E1pyrDb0945 JW0928 Dihydroorotate dehydrogenase (EC 1.3.5.2) (DHOdehase) (DHOD) (DHODase) (Dihydroorotate oxidase)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membrane | Peripheral membrane proteinCytoplasmicyesyesN450.146CYT0-0.200913-000iCytoplasmicMembrane1012.87
PYRG_ECOLIP0A7E5pyrGb2780 JW2751 CTP synthase (EC 6.3.4.2) (CTP synthetase) (UTP--ammonia ligase)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).PyrG=CTP synthase or CtpS in some systems. CTP synthase forms filaments in Caulobacter crescentus that regulate cell curvature independently of its catalytic function. The morphogenic role of CtpS requires its functional interaction with the intermediate filament, crescentin (CreS). Trigger factor-sensitive aggregation. [19737520] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20639870] [19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicTFyesyesN270.24SpI261.52462-100iCytoplasmic9.9777.31
PYRH_ECOLIP0A7E9pyrHsmbAb0171 JW0166 Uridylate kinase (UK) (EC 2.7.4.22) (Uridine monophosphate kinase) (UMP kinase) (UMPK)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Fluorescent fusions of PyrH have been monitored via fluorescence microscopy. PyrH was found to be located in one membrane-associated foci per cell. [17272300] PyrH-EYFP fusions are predominantly localized near the bacterial membranes of different cells undergoing septation and division. [9922246] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9922246] [17272300] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN230.14CYT0-0.200913-000iCytoplasmic9.9730.89
RBFA_ECOLIP0A7G2rbfAP15B yhbBb3167 JW3136 Ribosome-binding factor A (Protein P15B)Peripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Assembly cofactor of ribosome; associate with the 30S subunit. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN510.152CYT0-0.200913-000iCytoplasmic1068.01
RECA_ECOLIP0A7G6recAlexB recH rnmB tif umuB zabb2699 JW2669 Protein recA (Recombinase A)Peripheral inner membrane protein facing the cytoplasm Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544][19737520] [2228969] [25664544]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicTFnoyesN30.103CYT0-0.200913-000iCytoplasmic1088.16
RECF_ECOLIP0A7H0recFuvrFb3700 JW3677 DNA replication and repair protein recFPeripheral inner membrane protein facing the cytoplasm (potential). NucleoidExperimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. The prokaryotic protein recF is a single-stranded DNA-binding protein which also probably binds ATP. RecF is involved in DNA metabolism; it is required for recombinational DNA repair and for induction of the SOS response. [1542576][19766000] [1542576]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN320.128CYT0-0.200913-000iCytoplasmic9.976.3
RIBB_ECOLIP0A7J0ribBhtrPb3041 JW3009 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) (EC 4.1.99.12)Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinCytoplasmicyesyesN220.191CYT0-0.200913-000iCytoplasmic9.97116.29
RIMM_ECOLIP0A7X6rimMyfjAb2608 JW5413 Ribosome maturation factor rimM (21K)Cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Assembly cofactor of ribosome; associate with the 30S subunit. [11514519] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [11514519] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN390.124CYT0-0.200913-000iCytoplasmic9.97106.62
RNC_ECOLIP0A7Y0rncb2567 JW2551 Ribonuclease 3 (EC 3.1.26.3) (Ribonuclease III) (RNase III)Cytoplasm Peripheral inner membrane protein facing the cytoplasm.Copurifies with trigger factor in pull down assays. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][19737520] [4865702] [24438330]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicTFyesyesN560.133CYT0-0.200913-000iCytoplasmic10116.91
RPOA_ECOLIP0A7Z4rpoApez phs sezb3295 JW3257 DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) (EC 2.7.7.6) (RNA polymerase subunit alpha) (Transcriptase subunit alpha)Peripheral inner membrane protein facing the cytoplasm (experimental). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338][23230279] [21541338]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN250.163CYT0-0.200913-000iCytoplasmic9.97
RPOZ_ECOLIP0A800rpoZb3649 JW3624 DNA-directed RNA polymerase subunit omega (RNAP omega subunit) (EC 2.7.7.6) (RNA polymerase omega subunit) (Transcriptase subunit omega)Peripheral inner membrane protein facing the cytoplasm (experimental).The presence of omega subunit of RNA polymerase (RpoZ) is necessary for maturation of RNA polymerase. Experimentally verified gene-product. Methionine start codon is cleaved.[11531998] [9868784] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN350.113CYT0-0.200913-000iCytoplasmic8.9681.6
RRF_ECOLIP0A805frrrrfb0172 JW0167 Ribosome-recycling factor (RRF) (Ribosome-releasing factor)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN330.103CYT0-0.200913-000iCytoplasmic10109.54
METK_ECOLIP0A817metKmetXb2942 JW2909 S-adenosylmethionine synthase (AdoMet synthase) (EC 2.5.1.6) (MAT) (Methionine adenosyltransferase)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Fluorescent fusions of MetK have been monitored via fluorescence microscopy. Cells containing almost exclusively diffuse cytoplasmic MetK, or focused locations.[17272300] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN640.118CYT0-0.200913-000iCytoplasmic1047.82
GLYA_ECOLIP0A825glyAb2551 JW2535 Serine hydroxymethyltransferase (SHMT) (Serine methylase) (EC 2.1.2.1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN470.123CYT0-0.200913-000iCytoplasmic10
SUCC_ECOLIP0A836sucCb0728 JW0717 Succinyl-CoA ligase [ADP-forming] subunit beta (EC 6.2.1.5) (Succinyl-CoA synthetase subunit beta) (SCS-beta)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN320.181CYT0-0.200913-000iCytoplasmic8.9667.84
TIG_ECOLIP0A850tigb0436 JW0426 Trigger factor (TF)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm Translation unit [8633085] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451][23230279] [8633085] [20153451]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN210.128CYT0-0.200913-000iCytoplasmic8.9687.56
TPIS_ECOLIP0A858tpiAtpib3919 JW3890 Triosephosphate isomerase (TIM) (EC 5.3.1.1) (Triose-phosphate isomerase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN400.118CYT0-0.200913-000iCytoplasmic9.9796.89
TPX_ECOLIP0A862tpxyzzJb1324 JW1317 Thiol peroxidase (EC 1.11.1.-) (Scavengase P20)Peripheral inner membrane protein facing the cytoplasm (experimental). Periplasmic Periplasmic Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. [7499381] [9298646] Disulfide bond-containing protein [25664544][15004283] [19054092] [23230279] [7499381] [9298646] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/APeriplasmPeriplasmic (Experimental)yesyesN330.123CYT0-0.200913-000iPeriplasmic10
TALB_ECOLIP0A870talByaaKb0008 JW0007 Transaldolase B (EC 2.2.1.2)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN330.122CYT0-0.200913-000iCytoplasmic8.9684.93
YHBJ_ECOLIP0A894yhbJb3205 JW3172 UPF0042 nucleotide-binding protein yhbJPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN180.112CYT0-0.200913-000iUnknown7.3338.62
RIMP_ECOLIP0A8A8rimPyhbCb3170 JW5533 Ribosome maturation factor rimPPeripheral inner membrane protein facing the cytoplasmAssembly cofactor of ribosome; associate with the 30S subunit. [21529161]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN190.107CYT0-0.200913-000iCytoplasmic9.97111.47
YIHI_ECOLIP0A8H6yihIb3866 JW3837 UPF0241 protein yihIPeripheral inner membrane protein facing the cytoplasm.Assembly cofactor of ribosome; associates with Der.[21529161]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN430.101CYT0-0.200913-000iUnknown2108.97
RHLB_ECOLIP0A8J8rhlBmmrAb3780 JW3753 ATP-dependent RNA helicase rhlB (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm Peripheral inner membrane protein facing the cytoplasm. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][23230279] [8610017] [18337249] [24438330]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN560.132CYT0-0.200913-000iCytoplasmic9.97
PSD_ECOLIP0A8K1psdb4160 JW4121 Phosphatidylserine decarboxylase proenzyme (EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental). Phosphatidylserine decarboxylase is composed of two nonidentical subunits, alpha and beta, derived from a single proenzyme. The proenzyme is processed in a post-translational event into the heterodimer. This enzyme is unique among the known pyruvoyl-dependent decarboxylases because it is membrane-associated. [3042771] The phosphatidylserine decarboxylase (EC 4.1.1.65, phosphatidyl-L-serine carboxy-lyase) of Escherichia coli is associated with the cytoplasmic membrane and requires nonionic detergent for solubilization and detection of enzymatic activity [1495415] A strain of Escherichia coli bearing a hybrid plasmid containing the psd gene, starved for isoleucine by the addition of valine, produces amounts of phosphatidyl-serine decarboxylase, a membrane-bound enzyme, about 40-fold higher than wild type. [368058] The first phospholipid biosynthetic enzyme to be purified to homogeneity was Psd of E. coli. Beginning in the late 1960s purification of membrane proteins after solubilization with detergents was an increasingly popular endeavor. [22925633] [3042771] [5543671] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3042771] [1495415] [368058] [22925633] [5543671] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membrane | Peripheral membrane proteinCytoplasmic (Experimental)noyesN230.185CYT0-0.200913-000iCytoplasmicMembrane1032.79
SYS_ECOLIP0A8L1serSb0893 JW0876 Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--tRNA ligase) (SerRS) (Seryl-tRNA(Ser/Sec) synthetase)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation unit[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN670.1CYT0-0.200913-000iCytoplasmic1028.35
SYN_ECOLIP0A8M0asnStssb0930 JW0913 Asparaginyl-tRNA synthetase (EC 6.1.1.22) (Asparagine--tRNA ligase) (AsnRS)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation unit. Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN530.182CYT0-0.200913-000iCytoplasmic1070.21
SYT_ECOLIP0A8M3thrSb1719 JW1709 Threonyl-tRNA synthetase (EC 6.1.1.3) (Threonine--tRNA ligase) (ThrRS)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN330.154CYT0-0.200913-000iCytoplasmic1022.86
FETP_ECOLIP0A8P3yggXb2962 JW2929 Probable Fe(2+)-trafficking proteinPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally verified gene-product. Methionine start codon is cleaved. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9868784] [19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN410.14CYT0-0.200913-00Yn4-11c16/17oUnknown2107.94
RRAA_ECOLIP0A8R0rraAmenG yiiVb3929 JW3900 Regulator of ribonuclease activity APeripheral inner membrane protein facing the cytoplasm. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicnoyesN340.108CYT0-0.200913-000iCytoplasmic9.97
RPOC_ECOLIP0A8T7rpoCtabBb3988 JW3951 DNA-directed RNA polymerase subunit beta' (RNAP subunit beta') (EC 2.7.7.6) (RNA polymerase subunit beta') (Transcriptase subunit beta')Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN260.118CYT0-0.200913-000iCytoplasmic9.97
SYDP_ECOLIP0A8U0sydydrb2793 JW2764 Protein sydPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicyesyesN200.125CYT0-0.200913-000iCytoplasmicMembrane1059.48
RPOB_ECOLIP0A8V2rpoBgroN nitB rif ron stl stv tabDb3987 JW3950 DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN400.117CYT0-0.200913-000iCytoplasmic9.97
YJGA_ECOLIP0A8X0yjgAb4234 JW4193 UPF0307 protein yjgA (x96 protein)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm[23230279] [16980477]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN460.101CYT0-0.200913-000iCytoplasmic9.9781.82
YBGC_ECOLIP0A8Z3ybgCb0736 JW0726 Acyl-CoA thioester hydrolase YbgC (Acyl-CoA thioesterase) (EC 3.1.2.-)Peripheral inner membrane protein facing the cytoplasm.Phospholipid metabolism complex PlsB-PssA-YbgC-acp [16294310]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicyesyesN520.116CYT0-0.200913-000iCytoplasmic9.2670.38
RIMJ_ECOLIP0A948rimJb1066 JW1053 Ribosomal-protein-alanine acetyltransferase (EC 2.3.1.128) (Acetylating enzyme for N-terminal of ribosomal protein S5)Peripheral inner membrane protein facing the cytoplasm (experimental).Assembly cofactor of ribosome; associate with the Pre-30S subunit. Overexpression of RimJ suppresses defects in S5(G28D) mutations.[21529161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN330.107CYT0-0.200913-000iCytoplasmic10
FABB_ECOLIP0A953fabBfabCb2323 JW2320 3-oxoacyl-[acyl-carrier-protein] synthase 1 (EC 2.3.1.41) (3-oxoacyl-[acyl-carrier-protein] synthase I) (Beta-ketoacyl-ACP synthase I) (KAS I)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN250.163CYT0-0.200913-00Yn4-15c24/25oCytoplasmic1013.39
ALKH_ECOLIP0A955edahga kdgAb1850 JW1839 KHG/KDPG aldolase [Includes: 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) (2-keto-4-hydroxyglutarate aldolase) (KHG-aldolase); 2-dehydro-3-deoxy-phosphogluconate aldolase (EC 4.1.2.14) (2-keto-3-deoxy-6-phosphogluconate aldolase) (KDPG-aldolase) (PhospPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN300.126CYT0-0.200913-000iCytoplasmic1077.97
ALF1_ECOLIP0A991fbaBdhnAb2097 JW5344 Fructose-bisphosphate aldolase class 1 (EC 4.1.2.13) (Fructose-biphosphate aldolase class I) (FBP aldolase)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN640.115CYT0-0.200913-000iUnknown24.72
GLPC_ECOLIP0A996glpCb2243 JW2237 Anaerobic glycerol-3-phosphate dehydrogenase subunit C (G-3-P dehydrogenase)Peripheral inner membrane protein facing the cytoplasm. Membrane associated Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. [3286606] [3286606]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinMembrane associated (Experimental)yesyesN350.222CYT0-0.200913-000iCytoplasmicMembrane1013.54
FTNB_ECOLIP0A9A2ftnByecIb1902 JW1890 Ferritin-like protein 2Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN480.152CYT0-0.200913-000iCytoplasmic1082.57
FTSZ_ECOLIP0A9A6ftsZsfiB sulBb0095 JW0093 Cell division protein ftsZPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental) Cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental). Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. Assembles at midcell at the inner surface of the cytoplasmic membrane. SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. [8917533] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Over-expression of NlpI can interrupt the nucleoids division and the assembly of FtsZ at the septum, mediated by IbpA/IbpB, suggesting a role of the NlpI/IbpA/IbpB complex in the cell division. [25699035][1944597] [20497333] [12808143] [8917533] [23230279] [25699035]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN260.12CYT0-0.200913-000iCytoplasmicMembrane1079.85
G3P1_ECOLIP0A9B2gapAb1779 JW1768 Glyceraldehyde-3-phosphate dehydrogenase A (GAPDH-A) (EC 1.2.1.12)Peripheral inner membrane protein facing the cytoplasm (experimental). Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544][19737520] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicTFyesyesN210.121CYT0-0.200913-000iCytoplasmic9.9773.46
GLPA_ECOLIP0A9C0glpAb2241 JW2235 Anaerobic glycerol-3-phosphate dehydrogenase subunit A (G-3-P dehydrogenase) (EC 1.1.5.3)Peripheral inner membrane protein facing the cytoplasm. Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN620.155CYT0-0.200913-000iCytoplasmicMembrane105.6
DAPD_ECOLIP0A9D8dapDb0166 JW0161 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117) (Tetrahydrodipicolinate N-succinyltransferase) (THDP succinyltransferase) (THP succinyltransferase) (Tetrahydropicolinate succinylase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN240.195CYT0-0.200913-000iCytoplasmic1041.15
CYSB_ECOLIP0A9F3cysBb1275 JW1267 HTH-type transcriptional regulator cysB (Cys regulon transcriptional activator)Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1IM (predicted)CytoplasmCytoplasmicyesyesN240.141CYT0-0.200913-000iCytoplasmic108.39
ACEA_ECOLIP0A9G6aceAiclb4015 JW3975 Isocitrate lyase (ICL) (Isocitrase) (Isocitratase) (EC 4.1.3.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN300.126CYT0-0.200913-000iCytoplasmic1042.87
CFA_ECOLIP0A9H7cfacdfAb1661 JW1653 Cyclopropane-fatty-acyl-phospholipid synthase (CFA synthase) (Cyclopropane fatty acid synthase) (EC 2.1.1.79)Peripheral inner membrane protein facing the cytoplasm (experimental).Exhibites aberrant localization by microscopy in the absence of YidC.[22108384] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicYidCyesyesN380.183CYT0-0.200913-000iCytoplasmic1026.75
CHEZ_ECOLIP0A9H9cheZb1881 JW1870 Protein phosphatase CheZ (EC 3.1.3.-) (Chemotaxis protein CheZ)Peripheral inner membrane protein facing the cytoplasm (potential). CytoplasmExperimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [12644507][19766000] [12644507]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN660.129CYT0-0.200913-000iCytoplasmic10107.74
PHOL_ECOLIP0A9K3ybeZb0660 JW0657 PhoH-like proteinPeripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN680.101CYT0-0.200913-000iCytoplasmic9.9761.82
PHOU_ECOLIP0A9K7phoUnmpAb3724 JW3702 Phosphate transport system protein phoUPeripheral inner membrane protein facing the cytoplasm (potential) Peripheral inner membrane protein facing the cytoplasm (experimental).Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3536855] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN170.113CYT0-0.200913-000iCytoplasmic1094.15
SLYD_ECOLIP0A9K9slyDb3349 JW3311 FKBP-type peptidyl-prolyl cis-trans isomerase slyD (PPIase) (EC 5.2.1.8) (Histidine-rich protein) (Metallochaperone slyD) (Rotamase) (Sensitivity to lysis protein D) (WHP)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN310.112CYT0-0.200913-000iCytoplasmic10
DLDH_ECOLIP0A9P0lpdAlpdb0116 JW0112 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes) (Glycine cleavage system L protein)Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Trigger factor-sensitive aggregation. [6352260] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bridge (45 50) Disulfide bond-containing protein [25664544][19737520] [16079137] [6352260] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane proteinPeripheral membrane proteinCytoplasmic (Experimental)TFyesyesN230.176CYT0-0.200913-000iCytoplasmic1079.07
DEAD_ECOLIP0A9P6deaDcsdA mssB rhlDb3162 JW5531 Cold-shock DEAD box protein A (EC 3.6.4.13) (ATP-dependent RNA helicase deaD) (Translation factor W2)Septal ring protein. Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmTrigger factor-sensitive aggregation [19737520]. Contains a SPOR domain. [8552679][19737520] [19880599] [23230279] [8552679]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN350.151CYT0-0.200913-000iCytoplasmic1050.36
ARCA_ECOLIP0A9Q1arcAcpxC dye fexA msp seg sfrAb4401 JW4364 Aerobic respiration control protein ArcA (Dye resistance protein)Peripheral inner membrane protein facing the cytoplasm. Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[18792681] [23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicyesyesN280.105CYT0-0.200913-000iCytoplasmic1090.11
ACCD_ECOLIP0A9Q5accDdedB usgb2316 JW2313 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (ACCase subunit beta) (Acetyl-CoA carboxylase carboxyltransferase subunit beta) (EC 6.4.1.2)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation related.[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN180.112CYT0-0.200913-000iCytoplasmic9.9762.49
ADHE_ECOLIP0A9Q7adhEanab1241 JW1228 Aldehyde-alcohol dehydrogenase [Includes: Alcohol dehydrogenase (ADH) (EC 1.1.1.1); Acetaldehyde dehydrogenase [acetylating] (ACDH) (EC 1.2.1.10); Pyruvate-formate-lyase deactivase (PFL deactivase)]Peripheral inner membrane protein facing the cytoplasm (potential).Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19737520] [19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN560.127CYT0-0.200913-000iCytoplasmic9.2645.84
DHAS_ECOLIP0A9Q9asdhomb3433 JW3396 Aspartate-semialdehyde dehydrogenase (ASA dehydrogenase) (ASADH) (EC 1.2.1.11)Peripheral Inner membrane protein facing the cytoplasm (potential) Peripheral inner membrane protein facing the cytoplasm (experimental).No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicyesyesN440.197CYT0-0.200913-000iCytoplasmic9.2618.65
FTSE_ECOLIP0A9R7ftsEb3463 JW3428 Cell division ATP-binding protein ftsEPeripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Homolog to import ATP-binding protein MalK. Is the putative ATP-binding protein component of an ATP-binding cassette (ABC) transporter. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. [10048040] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10048040] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN320.118CYT0-0.200913-000iCytoplasmicMembrane1011.64
GATD_ECOLIP0A9S3gatDb2091 JW2075 Galactitol-1-phosphate 5-dehydrogenase (EC 1.1.1.251)Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicTFnoyesN530.115CYT0-0.200913-000iCytoplasmic9.9753.62
LIVG_ECOLIP0A9S7livGb3455 JW3420 High-affinity branched-chain amino acid transport ATP-binding protein livG (LIV-I protein G)Peripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN210.325CYT0-0.200913-000iCytoplasmic9.1211.51
YBBA_ECOLIP0A9T8ybbAb0495 JW0484 Uncharacterized ABC transporter ATP-binding protein ybbAPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN410.105CYT0-0.200913-000iCytoplasmicMembrane10102.82
YBHF_ECOLIP0A9U1ybhFb0794 JW5104 Uncharacterized ABC transporter ATP-binding protein ybhFPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN380.115CYT0-0.200913-000iCytoplasmicMembrane7.8842.12
YBIT_ECOLIP0A9U3ybiTb0820 JW0804 Uncharacterized ABC transporter ATP-binding protein ybiTPeripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN190.108CYT0-0.200913-000iCytoplasmic9.9742.79
LPTB_ECOLIP0A9V1lptByhbGb3201 JW3168 Lipopolysaccharide export system ATP-binding protein lptB (EC 3.6.3.-)Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[17056748] [16079137] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmic(experimental)yesyesN430.117CYT0-0.200913-000iCytoplasmicMembrane1019.08
YJJK_ECOLIP0A9W3yjjKb4391 JW4354 Uncharacterized ABC transporter ATP-binding protein yjjKPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN430.116CYT0-0.200913-000iCytoplasmic9.9792.13
ZNUC_ECOLIP0A9X1znuCyebMb1858 JW1847 Zinc import ATP-binding protein ZnuC (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN220.118CYT0-0.200913-000iCytoplasmicMembrane7.8815.58
MREB_ECOLIP0A9X4mreBenvB rodYb3251 JW3220 Rod shape-determining protein mreBPeripheral inner membrane protein. Peripheral inner membrane protein. Peripheral inner membrane protein. Peripheral inner membrane protein. Peripheral inner membrane protein. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Bacterial actin homologue; is required for cell shape maintenance in most non-spherical bacteria, where it assembles into helical structures just underneath the cytoplasmic membrane; binds to the bitopic inner membrane protein RodZ as monomer or multimer. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [19737520] PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones. [PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones. [24057063] [17090951] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20168300] [19078962] [21816350] [19008860] [16391237] [19737520] [24057063] [17090951] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)TFyesyesN410.127CYT0-0.200913-000iCytoplasmic9.9762.52
OMPR_ECOLIP0AA16ompRkmt ompBb3405 JW3368 Transcriptional regulatory protein ompRNucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[2668953] [15522865] [23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1Soluble IM FractionCytoplasmCytoplasmicyesyesN380.124CYT0-0.200913-000iCytoplasmic1087.16
THIO_ECOLIP0AA25trxAfipA tsnCb3781 JW5856 Thioredoxin-1 (Trx-1) Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Disulfide bridge (33 36) [10489448] Disulfide bond-containing protein [25664544][19737520] [10489448] [25664544] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFnoyesN200.119CYT0-0.200913-000iCytoplasmic9.2689.01
CLSB_ECOLIP0AA84clsBybhOb0789 JW0772 Putative cardiolipin synthase ybhO (CL synthase) (EC 2.7.8.-) In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. [22988102][22988102]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinCytoplasmicyesyesN660.136CYT0-0.200913-000iCytoplasmicMembrane7.8827.01
UBID_ECOLIP0AAB4ubiDyigC yigYb3843 JW3819 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-) (Polyprenyl p-hydroxybenzoate decarboxylase)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell membrane | Peripheral membrane proteinCytoplasmicyesyesN650.107CYT0-0.200913-000iCytoplasmic9.9737.33
USPD_ECOLIP0AAB8uspDyiiTb3923 JW3894 Universal stress protein DPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN540.108CYT0-0.200913-000iCytoplasmic9.9796.7
ARAG_ECOLIP0AAF3araGb1900 JW1888 Arabinose import ATP-binding protein AraG (EC 3.6.3.17)Peripheral inner membrane protein facing the cytoplasm (experimental).[7028715]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN380.293CYT0-0.200913-000iCytoplasmicMembrane1037.9
ARTP_ECOLIP0AAF6artPb0864 JW0848 Arginine transport ATP-binding protein ArtP (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). The artPIQM artJ genes encode a third arginine-uptake system in addition to the known argT hisJQMP system of Salmonella typhimurium and E. coli and the arginine (-ornithine) carrier (aps) of E. coli. The artQ, artM and artP products are similar to the transmembraneous proteins and the ATPase of binding-protein-dependent carriers. [8801422] [8801422] [15758229] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][8801422] [15758229] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN200.124CYT0-0.200913-000iCytoplasmicMembrane7.8826.62
DPPD_ECOLIP0AAG0dppDb3541 JW3510 Dipeptide transport ATP-binding protein dppDPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN660.286CYT0-0.200913-000iCytoplasmicMembrane7.8819.13
GLTL_ECOLIP0AAG3gltLb0652 JW0647 Protein maoC (Phenylacetic acid degradation protein paaZ)Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN270.108CYT0-0.200913-000iCytoplasmicMembrane9.9943.25
MGLA_ECOLIP0AAG8mglAb2149 JW2136 Galactose/methyl galactoside import ATP-binding protein MglA (EC 3.6.3.17)Inner membrane (experimental).[15919657]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN290.181CYT0-0.200913-000iCytoplasmicMembrane1014.72
PSTB_ECOLIP0AAH0pstBphoTb3725 JW3703 Phosphate import ATP-binding protein pstB (EC 3.6.3.27) (ABC phosphate transporter) (Phosphate-transporting ATPase)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3881386] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN410.133CYT0-0.200913-000iCytoplasmicMembrane1023.89
SAPD_ECOLIP0AAH4sapDb1291 JW1284 Peptide transport system ATP-binding protein sapDPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN530.145CYT0-0.200913-000iCytoplasmicMembrane7.8822.13
SAPF_ECOLIP0AAH8sapFb1290 JW1283 Peptide transport system ATP-binding protein sapFPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN450.122CYT0-0.200913-000iCytoplasmicMembrane7.888.32
SSUB_ECOLIP0AAI1ssuBycbEb0933 JW0916 Aliphatic sulfonates import ATP-binding protein SsuB (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN220.181CYT0-0.200913-000iCytoplasmicMembrane1023.53
FABF_ECOLIP0AAI5fabFfabJb1095 JW1081 3-oxoacyl-[acyl-carrier-protein] synthase 2 (EC 2.3.1.179) (3-oxoacyl-[acyl-carrier-protein] synthase II) (Beta-ketoacyl-ACP synthase II) (KAS II)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN480.158CYT0-0.200913-00Yn6-14c20/21oCytoplasmic9.268.31
FDOH_ECOLIP0AAJ5fdoHb3893 JW3864 Formate dehydrogenase-O iron-sulfur subunit (Aerobic formate dehydrogenase iron-sulfur subunit) (FDH-Z subunit beta) (Formate dehydrogenase-O subunit beta)Integral Inner Membrane Peripheral inner membrane protein facing the cytoplasm (experimental).Part of FDH-O isoenzyme complex whose physiological role is to ensure rapid adaptation during a shift from aerobiosis to anaerobiosis. FDH-O is a membrane-bound enzyme complex composed of three subunits, alpha (FdoG), beta (FdoH), and gamma (FdoI). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21890895] [9852007] [21541338] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell membrane | Single-pass membrane proteinMembrane anchoredyesyesN240.119CYT0-0.200913-000iCytoplasmicMembrane1077.94
YDHY_ECOLIP0AAL6ydhYb1674 JW1664 Uncharacterized ferredoxin-like protein ydhYPeripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicyesyesN280.128CYT0-0.200913-000iCytoplasmic9.2631.5
ALF_ECOLIP0AB71fbaAfba fdab2925 JW2892 Fructose-bisphosphate aldolase class 2 (FBP aldolase) (FBPA) (EC 4.1.2.13) (Fructose-1,6-bisphosphate aldolase) (Fructose-bisphosphate aldolase class II)Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmicTrigger factor-sensitive aggregation. [2649077][19737520] [23230279] [2649077]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmic (Experimental)TFyesyesN460.11CYT0-0.200913-000iCytoplasmic9.9769.89
KBL_ECOLIP0AB77kblb3617 JW3592 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (EC 2.3.1.29) (Glycine acetyltransferase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN340.12CYT0-0.200913-000iCytoplasmic9.9737.95
PUR8_ECOLIP0AB89purBb1131 JW1117 Adenylosuccinate lyase (ASL) (EC 4.3.2.2) (Adenylosuccinase) (ASase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN610.215CYT0-0.200913-000iCytoplasmic9.9774.4
ATPD_ECOLIP0ABA4atpHpapE uncHb3735 JW3713 ATP synthase subunit delta (ATP synthase F(1) sector subunit delta) (F-type ATPase subunit delta) (F-ATPase subunit delta)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [15919657] [21541338] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN360.135CYT0-0.200913-000iCytoplasmic1089.37
ATPG_ECOLIP0ABA6atpGpapC uncGb3733 JW3711 ATP synthase gamma chain (ATP synthase F1 sector gamma subunit) (F-ATPase gamma subunit)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [7508444] [2900236] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [21541338] [7508444] [2900236] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN410.162CYT0-0.200913-000iCytoplasmicMembrane1048.82
ATPA_ECOLIP0ABB0atpApapA uncAb3734 JW3712 ATP synthase subunit alpha (EC 3.6.3.14) (ATP synthase F1 sector subunit alpha) (F-ATPase subunit alpha)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [22108384] [16079137] [15919657] [21541338] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane proteinCytoplasmicTFYidCnoyesN280.106CYT0-0.200913-000iCytoplasmic1060.67
ATPB_ECOLIP0ABB4atpDpapB uncDb3732 JW3710 ATP synthase subunit beta (EC 3.6.3.14) (ATP synthase F1 sector subunit beta) (F-ATPase subunit beta)Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][22108384] [15919657] [21541338] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicYidCyesyesN230.123CYT0-0.200913-000iCytoplasmicMembrane1077.87
ACCA_ECOLIP0ABD5accAb0185 JW0180 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (ACCase subunit alpha) (Acetyl-CoA carboxylase carboxyltransferase subunit alpha) (EC 6.4.1.2)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN270.103CYT0-0.200913-000iCytoplasmic1028.79
PCNB_ECOLIP0ABF1pcnBb0143 JW5808 Poly(A) polymerase (PAP) (EC 2.7.7.19) (Plasmid copy number protein)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).RNA degradosome component/adds polyA tails to mRNAs targeted for degradation. The pcnB gene product, is the main enzyme responsible for RNA polyadenylation in E. coli. Polyadenylated RNA molecules are rapidly degraded by a multiprotein complex called RNA degradosome. PAP I localizes in the cytosol but also, like the RNA degradosome, in cellular membranes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19767441] [15737627] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN260.111CYT0-0.200913-000iCytoplasmic9.9723.32
FTSA_ECOLIP0ABH0ftsAdivAb0094 JW0092 Cell division protein ftsAPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (experimental).FtsA has a conserved C-terminal membrane targeting sequence (MTS) with extremely weak TmHMM and Phobius predictions. Deletion or point mutations in the MTS, inactivate FtsA function and inhibit cell division. Because FtsA binds to the tubulin-like FtsZ protein that forms the Z ring, it is thought that the MTS of FtsA is required, along with the transmembrane protein ZipA, to assemble the Z ring and anchor it to the cytoplasmic membrane. FtsA function in cell division depends on additive effects of membrane binding and self-interaction, and that the specific requirement of an amphipathic helix for tethering FtsA to the membrane can be bypassed. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18186792] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN250.12CYT0-0.200913-000iCytoplasmic9.9784.72
CISY_ECOLIP0ABH7gltAgluT icdBb0720 JW0710 Citrate synthase (EC 2.3.3.1)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN430.113CYT0-0.200913-000iCytoplasmic9.9722.15
CYSK_ECOLIP0ABK5cysKcysZb2414 JW2407 Cysteine synthase A (CSase A) (EC 2.5.1.47) (O-acetylserine (thiol)-lyase A) (OAS-TL A) (O-acetylserine sulfhydrylase A) (Sulfate starvation-induced protein 5) (SSI5)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN310.116CYT0-0.200913-000iCytoplasmic9.2677.42
DEOD_ECOLIP0ABP8deoDpupb4384 JW4347 Purine nucleoside phosphorylase deoD-type (PNP) (EC 2.4.2.1) (Inosine phosphorylase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN270.108CYT0-0.200913-000iCytoplasmic9.97108.87
DPS_ECOLIP0ABT2dpspexB vtmb0812 JW0797 DNA protection during starvation protein (EC 1.16.-.-)Nucleoid-associated protein (NAP) (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmicNucleoid-associated protein (NAP) Dodecameric Dps forms a protein cage with a hollow interior that sequesters Fe 2+ and reduces hydroxyl radical formation [23701194] [7984106][21541338] [23230279] [23701194] [7984106]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1Soluble IM FractionCytoplasm nucleoidCytoplasmic (Experimental)yesyesN530.125CYT0-0.200913-000iCytoplasmic1044.48
FLIG_ECOLIP0ABZ1fliGfla AII.2 fla BIIb1939 JW1923 Flagellar motor switch protein FliGPeripheral inner membrane protein facing the cytoplasm. Membrane associated Membrane associated Membrane associatedFlaggelar C-ring proteins - FliG, FliM, and FliN. MotA and MotB are ion-conducting membrane proteins that form the stator of the flagellum motor. FliG is a component of the rotor, present in about 25 copies per flagellum. The C-terminal domain of FliG is a component of the rotor in the bacterial flagellar motor. [10440379] [17085573] [16077109] [8631704][15546667] [10440379] [17085573] [16077109] [8631704]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideBacterial flagellum basal bodyMembrane associated (Experimental)flagellumnoyesN230.251CYT0-0.200913-000iCytoplasmic9.1280.75
KDSC_ECOLIP0ABZ4kdsCyrbI yrbJb3198 JW3165 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45) (KDO 8-P phosphatase)Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (potential).Short signal peptide of 17aa. Prediction by Phobius and the SignalP NN approach/extensive islands of hydrophobicity by K/D. No obvious biochemical reason that the enzyme should be secreted. Signal peptide like sequence could act as peripheral membrane association element. Lipopolysaccharide (LPS) is an amphiphilic molecule composed of a hydrophilic heteropolysaccharide and the membrane-embedded Lipid A. The innermost 8-carbon sugar 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) is the only conserved structural element found in all LPS, linking the lipid A to the carbohydrate domain of the molecule. The KDO 8-phosphate phosphatase (KdsC) is one of the enzymes of the KDO biosynthesis pathway and is responsible for cleavage of the phosphate group from KDO 8-phosphate to yield KDO. [12639950] In Escherichia coli, KdsC has been characterized as a homotetrameric molecule with a random-coiled C-terminal tail in each subunit. [12639950] [12639950][12639950] [19726684]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmic (Experimental)yesyesN180.326CYT0-0.200913-00Yn4-12c17/18oCytoplasmic9.9790.36
FUMA_ECOLIP0AC33fumAb1612 JW1604 Fumarate hydratase class I, aerobic (Fumarase) (EC 4.2.1.2)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN570.116CYT0-0.200913-000iCytoplasmic9.9737.19
ASPA_ECOLIP0AC38aspAb4139 JW4099 Aspartate ammonia-lyase (Aspartase) (EC 4.3.1.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Trigger factor-sensitive aggregation. Detected with chromosomally tap-tagged TF with >80% confidence score [15690043][19737520] [23230279] [15690043]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN220.123CYT0-0.200913-000iCytoplasmic9.9787.37
DHSA_ECOLIP0AC41sdhAb0723 JW0713 Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [9600841] [9740056] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [16079137] [12560550] [21541338] [9600841] [9740056] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmic (Experimental)TFyesyesN500.189CYT0-0.200913-000iCytoplasmicMembrane1010.16
FRDB_ECOLIP0AC47frdBb4153 JW4114 Fumarate reductase iron-sulfur subunit (EC 1.3.99.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).FrdA and FrdB fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). [PMC323229] [8419359] [6751816] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][PMC323229] [8419359] [6751816] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)noyesN280.105CYT0-0.200913-000iCytoplasmicMembrane9.8291.41
GLNK_ECOLIP0AC55glnKybaIb0450 JW0440 Nitrogen regulatory protein P-II 2Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic CytoplasmicEncodes a member of the PII signal transductio protein family, proteins that regulate enzyme activity and gene expression in response to the intracellular nitrogen status; cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the the ammonium transporter AmtB. [9720863] [10231487] [11847102] [14668330][15063852] [11847102] [9720863] [10231487] [14668330]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)yesyesN670.123CYT0-0.200913-000iCytoplasmicMembrane7.8841.19
DNAB_ECOLIP0ACB0dnaBgroP grpAb4052 JW4012 Replicative DNA helicase (EC 3.6.4.12)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicTFyesyesN30.105CYT0-0.200913-000iCytoplasmic9.9781.48
HEMG_ECOLIP0ACB4hemGyihBb3850 JW3827 Protoporphyrinogen IX dehydrogenase [menaquinone] (EC 1.3.5.3)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN330.11CYT0-0.200913-000iUnknown275.39
GLMU_ECOLIP0ACC7glmUyieAb3730 JW3708 Bifunctional protein glmU [Includes: UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) (N-acetylglucosamine-1-phosphate uridyltransferase); Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)]Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN490.123CYT0-0.200913-000iCytoplasmic1094.89
NIFU_ECOLIP0ACD4nifUicsU yfhNb2529 JW2513 NifU-like proteinPeripheral inner membrane protein facing the cytoplasm (experimental). IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein [25664544][10891064] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN200.105CYT0-0.200913-000iCytoplasmic9.2697.36
MBHL_ECOLIP0ACD8hyaBb0973 JW0955 Hydrogenase-1 large chain (HYD1) (EC 1.12.99.6) (Membrane-bound hydrogenase 1 large subunit) (NiFe hydrogenase)Peripheral inner membrane protein facing the cytoplasm. PeriplasmicPeripherar Inner Membrane Cyto side (Potential) Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. [22769583] [2180913][22769583] [2180913]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinPeriplasmic (Experimental)yesyesN490.102CYT0-0.200913-000iCytoplasmicMembrane109.28
DBHA_ECOLIP0ACF0hupAb4000 JW3964 DNA-binding protein HU-alpha (HU-2) (NS2)Nucleoid-associated protein (NAP) (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). NucleoidIdentified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature (PDOC00044).[21541338] [23230279] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN240.244CYT0-0.200913-000iCytoplasmic9.2696.48
DBHB_ECOLIP0ACF4hupBhopDb0440 JW0430 DNA-binding protein HU-beta (HU-1) (NS1)Nucleoid Peripheral inner membrane protein facing the cytoplasm (experimental). NucleoidIdentified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature [21541338] [23230279] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN270.114CYT0-0.200913-000iCytoplasmic9.26
HNS_ECOLIP0ACF8hnsbglY cur drdX hnsA msyA osmZ pilG topSb1237 JW1225 DNA-binding protein H-NS (Histone-like protein HLP-II) (Protein B1) (Protein H1)Peripheral inner membrane protein facing the cytoplasm (experimental).Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338].[9868784] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN190.173CYT0-0.200913-000iCytoplasmic10103.34
STPA_ECOLIP0ACG1stpAhnsBb2669 JW2644 DNA-binding protein stpA (H-NS homolog stpA)Nucleoid-associated protein (NAP) (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338].[21541338] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN190.184CYT0-0.200913-000iCytoplasmic9.2615.73
LRP_ECOLIP0ACJ0lrpalsB ihb livR oppIb0889 JW0872 Leucine-responsive regulatory proteinNucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1IM (predicted)CytoplasmicyesyesN480.101CYT0-0.200913-000iCytoplasmic9.9736.56
CRP_ECOLIP0ACJ8crpcap csmb3357 JW5702 Catabolite gene activator (cAMP receptor protein) (cAMP regulatory protein)Peripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid protein. Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[15650111] [23138451] [19737520] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicTFyesyesN240.105CYT0-0.200913-000iCytoplasmic9.97
RAIA_ECOLIP0AD49raiAyfiAb2597 JW2578 Ribosome-associated inhibitor A (Protein Y) (SpotY) (pY)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN630.108CYT0-0.200913-000iCytoplasmic1098.05
KPYK1_ECOLIP0AD61pykFb1676 JW1666 Pyruvate kinase I (EC 2.7.1.40) (PK-1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN420.167CYT0-0.200913-000iCytoplasmic9.97108.67
AMPH_ECOLIP0AD70ampHyaiHb0376 JW5052 Penicillin-binding protein AmpHInner membrane. Potential substrate of periplasmic peptidyl prolyl cis–trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein [25664544][18498364] [22001512] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/APeriplasmicSECSURAyesyesY220.613SpI2116.801-00Yn5-16c21/22oPeriplasmic9.7641.35
YGAU_ECOLIP0ADE6ygaUyzzMb2665 JW2640 Uncharacterized protein ygaUPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the periplasm (potential).Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane [12175925]. Homologous to OsmY.[21541338] [23230279] [12878000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN100.101CYT0-0.200913-000iUnknown289.97
SUHB_ECOLIP0ADG4suhBssyAb2533 JW2517 Inositol-1-monophosphatase (I-1-Pase) (IMPase) (Inositol-1-phosphatase) (EC 3.1.3.25)Peripheral inner membrane protein facing the cytoplasm (experimental).SuhB forms a tight complex with RNA polymerase (RNA pol) that inhibits the IMPase catalytic activity of SuhB[17652087] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN210.179CYT0-0.200913-000iCytoplasmic9.9786.55
IMDH_ECOLIP0ADG7guaBguaRb2508 JW5401 Inosine-5'-monophosphate dehydrogenase (IMP dehydrogenase) (IMPD) (IMPDH) (EC 1.1.1.205)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental).Prediction of one very weak transmembrane helix.[23230279] [15919657]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN290.14CYT0-0.200913-000iCytoplasmic9.9749.17
ENTB_ECOLIP0ADI4entBentGb0595 JW0587 Isochorismatase (EC 3.3.2.1) (2,3 dihydro-2,3 dihydroxybenzoate synthase) (Enterobactin synthase component B) (Enterochelin synthase B) (Isochorismate lyase)Peripheral inner membrane protein facing the cytoplasm (experimental). [10692387] [PMC94479]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN260.118CYT0-0.200913-000iCytoplasmic8.9665.54
YIEE_ECOLIP0ADM8yieEb3712 JW3690 Uncharacterized protein yieEPeripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicyesyesN250.109CYT0-0.200913-000iUnknown27.53
YIGI_ECOLIP0ADP2yigIb3820 JW5588 Uncharacterized protein yigIPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected), Soluble IM Fraction +CytoplasmicyesyesN280.137CYT0-0.200913-000iUnknown29.81
ZAPA_ECOLIP0ADS2zapAygfEb2910 JW2878 Cell division protein zapA (Z ring-associated protein zapA)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmBinds to the FtsZ ring. ZapA interacts directly with itself, FtsN, FtsZ and FtsI. Peripheral inner membrane protein via binding to the FtsZ ring. ZapA orthologue, YgfE, binds FtsZ polymers and promotes FtsZ polymer bundling. [12368265][20487275] [20497333] [17428494] [12368265]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN670.152CYT0-0.200913-000iUnknown297.8
YGIB_ECOLIP0ADT2ygiBb3037 JW5927 UPF0441 protein ygiBPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental). YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein [25664544][20545840] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN360.178SpII369.72422Pos+2=E00Yn21-32c37/38oPeriplasmic9.83
TRMJ_ECOLIP0AE01trmJyfhQb2532 JW2516 tRNA (cytidine/uridine-2'-O-)-methyltransferase trmJ (EC 2.1.1.-) (tRNA Cm32/Um32 methyltransferase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN630.12CYT0-0.200913-000iCytoplasmic9.9778.69
AHPC_ECOLIP0AE08ahpCb0605 JW0598 Alkyl hydroperoxide reductase subunit C (EC 1.11.1.15) (Alkyl hydroperoxide reductase protein C22) (Peroxiredoxin) (SCRP-23) (Sulfate starvation-induced protein 8) (SSI8) (Thioredoxin peroxidase)Peripheral inner membrane protein facing the cytoplasm (experimental). Disulfide bridge (47 47;166 166) Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN490.111CYT0-0.200913-000iCytoplasmic1090.35
MAP1_ECOLIP0AE18mapb0168 JW0163 Methionine aminopeptidase (MAP) (EC 3.4.11.18) (Peptidase M)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm Has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][23230279] [17272300]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN220.108CYT0-0.200913-000iCytoplasmic9.9763.72
CPXR_ECOLIP0AE88cpxRyiiAb3912 JW3883 Transcriptional regulatory protein CpxRPeripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[8294007] [15132751] [23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicyesyesN320.14CYT0-0.200913-000iCytoplasmic10118.97
USPA_ECOLIP0AED0uspAb3495 JW3462 Universal stress protein APeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential). Cytoplasmic Cytoplasmic Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).The Usp superfamily encompasses an ancient and conserved group of proteins that are found in bacteria, archaea,fungi, flies and plants. Usually each organism has several copies of Usp genes. Escherichia coli contains six Usp proteins, which can be divided into two subfamilies on the basis of sequence similarity. [19101563] Three of these proteins belong to the UspA subfamily (UspA, UspC, and UspD), and two to the UspFG subfamily (UspF and UspG). Another homologue is YbdQ. UspC binds to the membrane-integrated sensor kinase KdpD involved in K+ ion sensing. The same could be true for the other Usp proteins but we could not find direct evidence in the literature. [1453957] [8506346] [8740179] [9405142] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15911532] [19101563] [1453957] [8506346] [8740179] [9405142] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmic (Experimental)yesyesN330.153CYT0-0.200913-000iCytoplasmic1080.47
FABG_ECOLIP0AEK2fabGb1093 JW1079 3-oxoacyl-[acyl-carrier-protein] reductase (EC 1.1.1.100) (3-ketoacyl-acyl carrier protein reductase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN230.249CYT0-0.200913-000iCytoplasmic9.2693.23
FABI_ECOLIP0AEK4fabIenvMb1288 JW1281 Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (NADH-dependent enoyl-ACP reductase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicTFyesyesN280.245CYT0-0.200913-000iCytoplasmicMembrane1048.83
GYRA_ECOLIP0AES4gyrAhisW nalA parDb2231 JW2225 DNA gyrase subunit A (EC 5.99.1.3)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN360.106CYT0-0.200913-000iCytoplasmic9.9749.36
GYRB_ECOLIP0AES6gyrBacrB himB hisU nalC parA pcbAb3699 JW5625 DNA gyrase subunit B (EC 5.99.1.3)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN540.109CYT0-0.200913-000iCytoplasmic9.97
MIND_ECOLIP0AEZ3minDb1175 JW1164 Septum site-determining protein minD (Cell division inhibitor minD)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10096083] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN250.128CYT0-0.200913-000iCytoplasmicMembrane1095.09
MOAB_ECOLIP0AEZ9moaBchlA2b0782 JW0765 Molybdenum cofactor biosynthesis protein BPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN240.114CYT0-0.200913-000iCytoplasmic9.97105.47
RRAB_ECOLIP0AF90rraByjgDb4255 JW4212 Uncharacterized protein yjgDPeripheral inner membrane protein facing the cytoplasm. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][24438330]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN410.107CYT0-0.200913-000iCytoplasmic8.96
RIDA_ECOLIP0AF93ridAyjgFb4243 JW5755 UPF0076 protein yjgFPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral Inner membrane protein facing the cytoplasm (experimental)Experimentally verified gene-product. Methionine start codon is cleaved. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9868784] [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN210.115CYT0-0.200913-000iCytoplasmic9.2675.64
NUOB_ECOLIP0AFC7nuoBb2287 JW5875 NADH-quinone oxidoreductase subunit B (EC 1.6.99.5) (NADH dehydrogenase I subunit B) (NDH-1 subunit B) (NUO2)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19189973] [15037611] [23417064] [21822288] [7607227] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmic (Experimental)yesyesN620.1CYT0-0.200913-000iCytoplasmicMembrane9.8278.35
NUOE_ECOLIP0AFD1nuoEb2285 JW2280 NADH-quinone oxidoreductase subunit E (EC 1.6.99.5) (NADH dehydrogenase I subunit E) (NDH-1 subunit E) (NUO5)Peripheral inner membrane protein facing the cytoplasm (by function). Cytoplasmic Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex[19737520]. Thermus homologue: Q56221 (NQO2_THET8). Copurifies with trigger factor in pull down assays[19737520]. [11416161] [7607227] [7690854] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [23543743] [11416161] [7607227] [7690854] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)TFyesyesN670.103CYT0-0.200913-000iCytoplasmic9.9731.3
NUOI_ECOLIP0AFD6nuoIb2281 JW2276 NADH-quinone oxidoreductase subunit I (EC 1.6.99.5) (NADH dehydrogenase I subunit I) (NDH-1 subunit I) (NUO9) Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).[20732298] [21541338] [21822288] [23417064] [15037611] [19189973] [11416161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN270.17CYT0-0.200913-010o6-26iCytoplasmic9.1272.28
NUSA_ECOLIP0AFF6nusAb3169 JW3138 Transcription elongation protein nusA (L factor) (N utilization substance protein A)Nucleoid Peripheral inner membrane protein facing the cytoplasm (experimental). Fluorescent fusions of NusA have been monitored via fluorescence microscopy. NusA was found to be located predominantly in two clusters associated with each nucleoid. NusA can self-assemble into oligomers under heat shock conditions. NusA also acquires chaperone activity.[17272300] [23230279] [PMC3731644]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicyesyesN410.104CYT0-0.200913-000iCytoplasmic9.9799.46
NUSG_ECOLIP0AFG0nusGb3982 JW3945 Transcription antitermination protein nusGPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN260.108CYT0-0.200913-000iCytoplasmic9.9772.87
ODO1_ECOLIP0AFG3sucAb0726 JW0715 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279] [15919657]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN240.133CYT0-0.200913-000iCytoplasmic9.976.61
ODO2_ECOLIP0AFG6sucBb0727 JW0716 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) (2-oxoglutarate dehydrogenase complex component E2) (OGDC-E2) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase cPeripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). CytoplasmicTrigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [9298646][19737520] [23230279] [15919657] [9298646]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)TFyesyesN190.112CYT0-0.200913-000iCytoplasmic9.97122.42
ODP1_ECOLIP0AFG8aceEb0114 JW0110 Pyruvate dehydrogenase E1 component (EC 1.2.4.1)Peripheral inner membrane protein facing the cytoplasm. Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).AceE is the E1 subunit of the 10MDa Escherichia coli pyruvate dehydrogenase multienzyme complex, which catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO(2) and acetate (crystal structure solved as a dimer [11955070]). The E1 subunit is present as a 24mer within the complex. AceE is also part of a very large complex partially resolved by cryoEM [16338405]. Analogous enzyme pyryvate oxidase-PoxB is membrane associated [18988747]. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16338405] [18988747] [2699406] [11955070] [19737520] [12488465] [15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicTFyesyesN530.105CYT0-0.200913-000iCytoplasmic9.9715.51
PARC_ECOLIP0AFI2parCb3019 JW2987 DNA topoisomerase 4 subunit A (EC 5.99.1.-) (Topoisomerase IV subunit A)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Dimer of topoisomerase IV subunit A (bioCyc) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1334483] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACell membrane | Peripheral membrane proteinCytoplasmicyesyesN180.118CYT0-0.200913-000iCytoplasmicMembrane1017.79
PDXH_ECOLIP0AFI7pdxHb1638 JW1630 Pyridoxine/pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx) (Pyridoxal 5'-phosphate synthase)Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN430.121CYT0-0.200913-000iCytoplasmic9.9751.36
PHOB_ECOLIP0AFJ5phoBb0399 JW0389 Phosphate regulon transcriptional regulatory protein phoBPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid (experimental).PhoB is a dual transcription regulator that activates expression of the Pho regulon in response to environmental Pi. Under phosphate limitation conditions the inner membrane sensor kinase PhoR autophosphorylates; Subsequent transfer of the phosphate group to PhoB results in activation of PhoB. It has been shown that PhoBR and PhoB are responsible for upregulation of phosphonate and glycerol phosphate metabolism and the high-affinity phosphate transport system, respectively, thus showing the complex regulation by the PhoR-PhoB two-component regulatory system.[2693738] [18031348] [23138451]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicyesyesN330.201CYT0-0.200913-000iCytoplasmic9.97
PPX_ECOLIP0AFL6ppxb2502 JW2487 Exopolyphosphatase (ExopolyPase) (EC 3.6.1.11) (Metaphosphatase)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell membrane | Peripheral membrane proteinCytoplasmicyesyesN290.112CYT0-0.200913-000iCytoplasmic1047.13
PSPA_ECOLIP0AFM6pspAb1304 JW1297 Phage shock protein APeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Forms large oligomers and binds directly to phospholipids (preferentially phosphatidylserine and phosphatidylglycerol). PMC3709507 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17725563] [19555453] [PMC3709507] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicProphage TransposaseyesnoN230.112CYT0-0.200913-000iCytoplasmicMembrane10113.75
YBBO_ECOLIP0AFP4ybbOb0493 JW0482 Uncharacterized oxidoreductase ybbO (EC 1.-.-.-)Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1Soluble IM FractionCytoplasmicnoyesN450.12CYT0-0.200913-010o240-258iCytoplasmic9.97
PSPD_ECOLIP0AFV8pspDb1307 JW1300 Uncharacterized protein ygcLPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12562786] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicProphage TransposasenonoN640.182CYT0-0.200913-000iUnknown234.95
SEQA_ECOLIP0AFY8seqAb0687 JW0674 Protein seqA Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Membrane-associated oriC-binding machinery of E. coli is a multiprotein complex that includes the SeqA and SeqB proteins. [9660922] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][7553853] [19737520] [16236133] [12535065] [9736699] [9660922] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN430.12CYT0-0.200913-000iCytoplasmic8.9613.08
SSPB_ECOLIP0AFZ3sspBb3228 JW3197 Stringent starvation protein BPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN470.112CYT0-0.200913-000iCytoplasmic8.96
PUR1_ECOLIP0AG16purFb2312 JW2309 Amidophosphoribosyltransferase (ATase) (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (GPATase)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN320.119CYT0-0.200913-000iCytoplasmic9.9715.84
RELA_ECOLIP0AG20relAb2784 JW2755 GTP pyrophosphokinase (EC 2.7.6.5) ((p)ppGpp synthase) (ATP:GTP 3'-pyrophosphotransferase) (ppGpp synthase I)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN370.119CYT0-0.200913-000iCytoplasmic8.9675.6
RHO_ECOLIP0AG30rhonitA psuA rnsC sbaA tsub3783 JW3756 Transcription termination factor Rho (EC 3.6.4.-) (ATP-dependent helicase Rho)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN440.103CYT0-0.200913-000iCytoplasmic9.97
SECB_ECOLIP0AG86secBb3609 JW3584 Protein-export protein SecBPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Cytoplasmic protein that forms transient interactions with the membrane; binds to the C-terminus of SecA. Protein that acts as its membrane receptor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9767586] [2170023] [PMC1326294] [6403503] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN530.133CYT0-0.200913-000iCytoplasmic1088.86
RPOE_ECOLIP0AGB6rpoEsigEb2573 JW2557 RNA polymerase sigma-E factor (Sigma-24)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane protein (experimental). Nucleoid (experimental). Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid (experimental). Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).SigmaE controls the σE-dependent extracytoplasmic stress response and is required to maintain cell envelope integrity in Escherichia coli [9159523] [18253509]. RseA, encodes an anti-sigma(E) activity demonstrated at both the genetic and biochemical levels [9159522]. RseC is an inner membrane protein that positively modulates sigma(E) activity [9159522]. σE is held at the membrane by the antisigma factor RseA. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB binds to the periplasmic domain of RseA and protects RseA from proteolysis. [17360428]. Unfolded OMPs activate the protease DegS, which cleave RpoE regulon [15520285]. Identified in the inner membrane by biochemical followed by two-dimensional gel electrophoresis and tandem mass spectrometry [15911532]. [15774872] [16597971] [11777003] Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase. [12718891] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][9159523] [9159522] [15911532] [15374527] [18253509] [20971899] [23138451] [15520285] [7889934] [17360428] [11777003] [15774872] [16597971] [12718891] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1Soluble IM FractionCytoplasmic (Experimental)yesyesN440.111CYT0-0.200913-000iCytoplasmic10101.13
SRP54_ECOLIP0AGD7ffhb2610 JW5414 Signal recognition particle protein (Fifty-four homolog) (p48)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18281057] [19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN630.1CYT0-0.200913-000iUnknown5.6106.77
SUCD_ECOLIP0AGE9sucDb0729 JW0718 Succinyl-CoA ligase [ADP-forming] subunit alpha (EC 6.2.1.5) (Succinyl-CoA synthetase subunit alpha) (SCS-alpha)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN310.171CYT0-0.200913-000iCytoplasmic9.9770.36
TDCB_ECOLIP0AGF6tdcBb3117 JW3088 Threonine dehydratase catabolic (EC 4.3.1.19) (Threonine deaminase)Peripheral Inner membrane protein facing the cytoplasm (potential) No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential substrate of periplasmic chaperone YfgM). [25403562] Putative substrate for the periplasmic chaperone YfgM. [25403562] [25403562] [25403562]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicYfgMyesyesN230.152CYT0-0.200913-000iCytoplasmic9.9772.45
TRKA_ECOLIP0AGI8trkAb3290 JW3251 Trk system potassium uptake protein trkA (K(+)-uptake protein trkA)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[2674131] [21605551] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicnoyesN170.13CYT0-0.200913-00Yn3-11c16/17oCytoplasmicMembrane1068.79
YFIF_ECOLIP0AGJ5yfiFb2581 JW2565 Uncharacterized tRNA/rRNA methyltransferase yfiF (EC 2.1.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFnoyesN450.103CYT0-0.200913-000iCytoplasmic9.9781.19
SYY_ECOLIP0AGJ9tyrSb1637 JW1629 Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosine--tRNA ligase) (TyrRS)Peripheral inner membrane protein facing the cytoplasm (experimental).Translation unit[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN650.123CYT0-0.200913-000iCytoplasmic1063.48
RSME_ECOLIP0AGL7rsmEyggJb2946 JW2913 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) (16S rRNA m3U1498 methyltransferase)Peripheral inner membrane protein facing the cytoplasm (experimental).Ribosomal[23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmCytoplasmicyesyesN400.129CYT0-0.200913-000iCytoplasmic8.9618.96
RLME_ECOLIP0C0R7rlmEftsJ mrsF rrmJb3179 JW3146 Ribosomal RNA large subunit methyltransferase E (EC 2.1.1.-) (23S rRNA Um2252 methyltransferase) (Cell division protein ftsJ) (rRNA (uridine-2'-O-)-methyltransferase)Peripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Assembly cofactor of ribosome. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN290.111CYT0-0.200913-000iCytoplasmic9.9716.33
GATY_ECOLIP0C8J6gatYyegFb2096 JW5343 D-tagatose-1,6-bisphosphate aldolase subunit gatY (TBPA) (TagBP aldolase) (EC 4.1.2.40) (D-tagatose-bisphosphate aldolase class II) (Tagatose-bisphosphate aldolase)Peripheral inner membrane protein facing the cytoplasm (potential).Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19737520] [19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN490.137CYT0-0.200913-000iCytoplasmic9.9711.46
GATZ_ECOLIP0C8J8gatZb2095 JW2082 D-tagatose-1,6-bisphosphate aldolase subunit gatZPeripheral inner membrane protein facing the cytoplasm (experimental). Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein [25664544][8955298] [19737520] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN330.318CYT0-0.200913-000iCytoplasmic8.966.6
EFTU1_ECOLIP0CE47tufAb3339 JW3301 Elongation factor Tu 1 (EF-Tu 1) (P-43)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. [7525272] [7021545] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451] Disulfide bond-containing protein [25664544][20133608] [2022614] [775340] [7525272] [7021545] [20153451] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCell membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN470.197CYT0-0.200913-000iCytoplasmic1099.39
EFTU2_ECOLIP0CE48tufBb3980 JW3943 Elongation factor Tu 2 (EF-Tu 2) (P-43)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). [20133608] [2022614] [775340] [23230279] [20153451]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell membrane | Peripheral membrane proteinCytoplasmicyesyesN470.197CYT0-0.200913-000iCytoplasmic1087.23
RCSB_ECOLIP0DMC7rcsBb2217 JW2205 Ankyrin repeat protein A (Ankyrin-like regulatory protein)Peripheral inner membrane protein facing the cytoplasm (experimental). NucleoidRegulator protein RcsB is a member of the two-component regulatory system RcsC/RcsB. It is involved in the activation of colanic acid capsule synthesis (cps) and cell division (ftsZ) genes. It is believed that the RcsB protein is activated through phosphorylation, probably by the sensor protein RcsC. RcsB protein for Regulator capsule synthesis B, is a response regulator that belongs to the multicomponent RcsF/RcsC/RcsD/RcsA-RcsB phosphorelay system. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). [2404948] [10702265] [9209051] [17185552] [14514676] [23138451] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicnoyesN660.117CYT0-0.200913-000iCytoplasmic1090.28
FTSY_ECOLIP10121ftsYb3464 JW3429 Cell division protein ftsYPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Contains an essential and autonomous membrane-binding amphipathic helix. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17726012] [18281057] [8194520] [9177162] [11353766] [17726013] [19414018] [21543314] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN30.107CYT0-0.200913-000iCytoplasmicMembrane10
GLNQ_ECOLIP10346glnQb0809 JW0794 Glutamine transport ATP-binding protein glnQPeripheral inner membrane protein facing the cytoplasm (experimental). EchoLOCATION based on the same reference says cytoplasmic. Uniprot gives the same reference but says peripheral. Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN420.105CYT0-0.200913-000iCytoplasmicMembrane1097.03
ENTE_ECOLIP10378entEb0594 JW0586 Enterobactin synthase component E (Enterochelin synthase E) [Includes: 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) (Dihydroxybenzoic acid-activating enzyme); S-dihydroxybenzoyltransferase (EC 2.3.1.-)]Peripheral inner membrane protein facing the cytoplasm (experimental). Homologous to FadD13 protein of Mycobacterium that is peripheral. Phobius predits transmembrane region around aa100. Binds AMP. Very high homology to eight other transport proteins: (FADK_ECOLI, CAIC_ECOLI, LCFA_ECOLI, ENTF_ECOLI, ACSA_ECOLI) Membrane Association of the Escherichia coli Enterobactin Synthase Proteins EntB/G, EntE, and EntF[10692387] [22560731] [PMC94479]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN360.15CYT0-0.200913-010o86-105iCytoplasmic9.9788.29
SECA_ECOLIP10408secAazi pea prlDb0098 JW0096 Protein translocase subunit secAInner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmCytoplasmicnoyesN700.109CYT0-0.200913-000iCytoplasmicMembrane1058.49
LPXB_ECOLIP10441lpxBpgsBb0182 JW0177 Lipid-A-disaccharide synthase (EC 2.4.1.182)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer[19883124]. It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer. [19883124] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][3905795] [19883124] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN230.146CYT0-0.200913-000iCytoplasmic1047.47
UGPC_ECOLIP10907ugpCb3450 JW3415 sn-glycerol-3-phosphate import ATP-binding protein UgpC (EC 3.6.3.20)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN370.105CYT0-0.200913-000iCytoplasmicMembrane1020.09
LIT_ECOLIP11072litb1139 JW1125 Bacteriophage T4 late gene expression-blocking protein (GpLit)Single weak hydrophobic region. Could be a transmembrane domain but could also be a peripheral inner membrane protein.Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicProphage Integrase (EcoGene)nonoN200.181CYT0-0.200913-010i61-81oCytoplasmic8.96
NARH_ECOLIP11349narHb1225 JW1216 Respiratory nitrate reductase 1 beta chain (EC 1.7.99.4) (Nitrate reductase A subunit beta) (Quinol-nitrate oxidoreductase subunit beta)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. [12910261] In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. [19295650] When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. [2674654] The heterotrimeric NarGHI has an αβγ structure encoded by narGHI respectively. The α and β subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane γ subunit. [3371362] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe–S] clusters and molybdenum in a sequential manner. Insertion of the [Fe–S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe–S] clusters and molybdenum in a sequential manner. Insertion of the [Fe–S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] [3053688] [2233673] [2674654] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12910261] [19295650] [2674654] [3371362] [16286471] [16286471] [3053688] [2233673] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN320.117CYT0-0.200913-000iCytoplasmicMembrane1013.23
ARLY_ECOLIP11447argHb3960 JW3932 Argininosuccinate lyase (ASAL) (EC 4.3.2.1) (Arginosuccinase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN530.138CYT0-0.200913-000iCytoplasmic9.97
ENTF_ECOLIP11454entFb0586 JW0578 Enterobactin synthase component F (EC 2.7.7.-) (Enterochelin synthase F) (Serine-activating enzyme) (Seryl-AMP ligase)Peripheral inner membrane protein facing the cytoplasm (experimental). [10692387] [PMC94479]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN560.235CYT0-0.200913-000iCytoplasmic9.9720.96
BGLG_ECOLIP11989bglGbglCb3723 JW3701 Cryptic beta-glucoside bgl operon antiterminatorPeripheral inner membrane protein facing the cytoplasmBglG is a transcriptional antiterminator which controls the expression bgl operon. It has been detected at the membrane when it is expressed with BglF, when expressed as monocistron, the bglG transcripts were observed at the cell poles (unpublished data). In the absence of β-glucosidase, BglG also assembles near membrane and forms a pro-form complex with BglF that maintains BglG inactive. In the presence of sugar, BglG associates with the proteins of the sugar phosphotransfer system (PTS) and localizes at the cell poles. BglG requires two distinct (de)phosphorylation events to gain activity.[22984181] [21278164]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN380.103CYT0-0.200913-000iCytoplasmic8.9648.58
METH_ECOLIP13009metHb4019 JW3979 Methionine synthase (EC 2.1.1.13) (5-methyltetrahydrofolate--homocysteine methyltransferase) (Methionine synthase, vitamin-B12-dependent) (MS)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN350.124CYT0-0.200913-000iCytoplasmic9.97
GLPB_ECOLIP13033glpBb2242 JW2236 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (Anaerobic G-3-P dehydrogenase subunit B) (Anaerobic G3Pdhase B) (EC 1.1.5.3)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. [6387427] Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. [1987111] [PMC3210124] [3286606][20706981] [6387427] [1987111] [PMC3210124] [3286606]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN300.137CYT0-0.200913-00Yn10-21c28/29oCytoplasmicMembrane1024.58
GLPD_ECOLIP13035glpDglyDb3426 JW3389 Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Binds to the cytoplasmic membrane through an amphipathic C-terminal alpha-helix (357-374)[11955283]. Identified in PlsB-PflB-GlpD-Psd [20521846]. Detergent-depleted enzyme can be functionally reconstituted with Escherichia coli membrane vesicles to support glycerol-3-phosphate-dependent active transport of L-proline[340460]. When GlpD is lost by mutation, the cells not only fail to grow on glycerol or l-alpha-glycerophosphate, but are subject to growth inhibition in the presence of either compound [5321485]. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [8982270] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11955283] [20521846] [340460] [5321485] [15919657] [15911532] [8982270] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmic (Experimental)yesyesN350.138CYT0-0.200913-000iCytoplasmic1048.25
RPOS_ECOLIP13445rpoSappR katF nur otsX sigSb2741 JW5437 RNA polymerase sigma factor rpoS (Sigma-38)Peripheral inner membrane protein facing the cytoplasm (experimental).[23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicnoyesN690.1CYT0-0.200913-000iCytoplasmic1030.82
PROV_ECOLIP14175proVb2677 JW2652 Glycine betaine/L-proline transport ATP-binding protein proVPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16079137] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN240.108CYT0-0.200913-000iCytoplasmicMembrane1073.2
FECE_ECOLIP15031fecEb4287 JW4247 Fe(3+) dicitrate transport ATP-binding protein fecE (Iron(III) dicitrate transport ATP-binding protein fecE)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN620.148CYT0-0.200913-000iCytoplasmicMembrane104.5
FLIN_ECOLIP15070fliNflaN motDb1946 JW1930 Flagellar motor switch protein FliNFlaggelar C-ring proteins - FliG, FliM, and FliN.[15546667]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane protein | Cytoplasmic sideBacterial flagellum basal bodyCytoplasmicflagellumnoyesN350.119CYT0-0.200913-000iCytoplasmicMembrane10107.99
MALQ_ECOLIP15977malQmalAb3416 JW3379 4-alpha-glucanotransferase (EC 2.4.1.25) (Amylomaltase) (Disproportionating enzyme) (D-enzyme)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN230.214SpI220.437923-000iCytoplasmic1031.98
SDHL_ECOLIP16095sdaAb1814 JW1803 L-serine dehydratase 1 (SDH 1) (EC 4.3.1.17) (L-serine deaminase 1) (L-SD1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN200.136CYT0-0.200913-000iCytoplasmic9.9771.35
HYCE_ECOLIP16431hycEhevEb2721 JW2691 Formate hydrogenlyase subunit 5 (FHL subunit 5) (Hydrogenase-3 component E)Peripheral inner membrane protein (potential).Nickel-containing large subunit of Hydrogenase 3 (hycABCDEF-GHI) that forms a loose association with the molyb- doenzyme formate dehydrogenase-H on the cytoplasmic face of the cytoplasmic membrane, where together they make up the formate hydrogenlyase complex.[1625581]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN270.119CYT0-0.200913-000iCytoplasmic9.127.11
SYP_ECOLIP16659proSdrpAb0194 JW0190 Prolyl-tRNA synthetase (EC 6.1.1.15) (Global RNA synthesis factor) (Proline--tRNA ligase) (ProRS)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN190.254CYT0-0.200913-000iCytoplasmic1016.89
CYSA_ECOLIP16676cysAb2422 JW2415 Sulfate/thiosulfate import ATP-binding protein cysA (EC 3.6.3.25) (Sulfate-transporting ATPase)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN330.112CYT0-0.200913-000iCytoplasmicMembrane7.8814.16
PHNC_ECOLIP16677phnCb4106 JW4067 Phosphonates import ATP-binding protein PhnC (EC 3.6.3.28)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN230.137CYT0-0.200913-000iCytoplasmicMembrane1010.59
PHNK_ECOLIP16678phnKb4097 JW5727 Phosphonates transport ATP-binding protein phnKPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN230.117CYT0-0.200913-000iCytoplasmicMembrane7.886.82
PHNL_ECOLIP16679phnLb4096 JW4057 Phosphonates transport ATP-binding protein phnLPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN340.263CYT0-0.200913-000iCytoplasmicMembrane9.828.15
RHSB_ECOLIP16917rhsBb3482 JW5679 Protein rhsBPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicRhs ElementnonoN460.116CYT0-0.200913-000iExtracellular9.64
GLMS_ECOLIP17169glmSb3729 JW3707 Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (D-fructose-6-phosphate amidotransferase) (GFAT) (Glucosamine-6-phosphate synthase) (Hexosephosphate aminotransferase) (L-glutamine-D-fructose-6-phosphate amidotransferase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmCytoplasmicTFnoyesN590.122CYT0-0.200913-000iCytoplasmic1091.22
MURG_ECOLIP17443murGb0090 JW0088 UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227) (Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase)Peripheral inner membrane protein facing the cytoplasm (experimental). Membrane associated Peripheral inner membrane protein facing the cytoplasm (experimental).Pyrophosphoryl-undecaprenol N-acetylglucosamine transferase. Murein synthesis peripheral membrane protein interacting with cardiolipin. [8449890] [10892798] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][8449890] [10892798] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinMembrane associated (Experimental)yesyesN270.156TMH03.61924-000iCytoplasmicMembrane1048.93
BETA_ECOLIP17444betAb0311 JW0303 Choline dehydrogenase (CDH) (CHD) (EC 1.1.99.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][22108384] [3512525] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinCytoplasmicYidCnoyesN190.163CYT0-0.200913-000iCytoplasmicMembrane108.8
CYSH_ECOLIP17854cysHb2762 JW2732 Phosphoadenosine phosphosulfate reductase (EC 1.8.4.8) (3'-phosphoadenylylsulfate reductase) (PAPS reductase, thioredoxin dependent) (PAPS sulfotransferase) (PAdoPS reductase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN590.168CYT0-0.200913-000iCytoplasmic1011.91
MURC_ECOLIP17952murCb0091 JW0089 UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM Fraction +CytoplasmCytoplasmicTFyesyesN410.228CYT0-0.200913-000iCytoplasmic9.9776.65
NADE_ECOLIP18843nadEefg ntrLb1740 JW1729 NH(3)-dependent NAD(+) synthetase (EC 6.3.1.5) (Nicotinamide adenine dinucleotide synthetase) (NADS) (Nitrogen regulatory protein)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN200.146CYT0-0.200913-000iCytoplasmic9.97
NARY_ECOLIP19318narYb1467 JW1462 Respiratory nitrate reductase 2 beta chain (EC 1.7.99.4)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Peripheral membrane proteinCytoplasmicyesyesN320.124CYT0-0.200913-000iCytoplasmicMembrane1014.16
NARZ_ECOLIP19319narZb1468 JW1463 Respiratory nitrate reductase 2 alpha chain (EC 1.7.99.4)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membrane | Peripheral membrane proteinCytoplasmicyesyesN200.145CYT0-0.200913-000iCytoplasmicMembrane10
FADB_ECOLIP21177fadBoldBb3846 JW3822 Fatty acid oxidation complex subunit alpha [Includes: Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC 4.2.1.17) (EC 5.1.2.3) (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN310.13CYT0-0.200913-000iCytoplasmic9.9715.16
RNR_ECOLIP21499rnrvacB yjeCb4179 JW5741 Ribonuclease R (RNase R) (EC 3.1.-.-) (Protein vacB)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN30.1CYT0-0.200913-000iCytoplasmic9.9745.49
SRMB_ECOLIP21507srmBrbaBb2576 JW2560 ATP-dependent RNA helicase srmB (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental).Assembly cofactor of ribosome; associate with the 50S subunit.[21529161] [23230279] [15919657]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN330.109CYT0-0.200913-000iCytoplasmic1068.57
RNE_ECOLIP21513rneams hmp1b1084 JW1071 Ribonuclease E (RNase E) (EC 3.1.26.12)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane protein (experimental). Cytoplasm. Cell inner membrane. Peripheral membrane protein. Cytoplasmic side. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm.Membrane-binding specificity of NRne was confirmed using circular dichroism difference spectroscopy. RnE binds specifically to anionic phospholipids through electrostatic interactions through N-terminal region (NRne, residues 1-499). Identified DsbC substrate [19953543] Identified DsbA substrate [19953543] [2011493] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][18976283] [15911532] [22509045] [2011493] [23230279] [24438330]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmic (Experimental)yesyesN620.101CYT0-0.200913-000iCytoplasmic10
KPYK2_ECOLIP21599pykAb1854 JW1843 Pyruvate kinase II (EC 2.7.1.40) (PK-2)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN430.131CYT0-0.200913-000iCytoplasmic9.9743.65
DBPA_ECOLIP21693dbpAb1343 JW1337 ATP-independent RNA helicase dbpA (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasmAssembly cofactor of ribosome; Fragment of 23S rRNA. Assembly cofactor of ribosome; Fragment of 23S rRNA. [21529161][21529161] [21529161]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN320.341CYT0-0.200913-000iCytoplasmic9.9712.57
SYD_ECOLIP21889aspStlsb1866 JW1855 Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase) (AspRS)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).SecY-interacting protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19139097] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN270.104CYT0-0.200913-000iCytoplasmic1081.96
ASNB_ECOLIP22106asnBb0674 JW0660 Asparagine synthetase B [glutamine-hydrolyzing] (EC 6.3.5.4)Peripheral inner membrane protein facing the cytoplasm (experimental).Copurifies with trigger factor in pull down assays.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN370.141CYT0-0.200913-000iCytoplasmic9.97
CYSQ_ECOLIP22255cysQamtAb4214 JW4172 3'(2'),5'-bisphosphate nucleotidase cysQ (EC 3.1.3.7) (3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase) (3'-phosphoadenosine 5'-phosphate phosphatase) (PAP phosphatase) (DPNPase)Peripheral inner membrane protein facing the cytoplasm. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [15911532][15911532]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideMembrane associated (Experimental)noyesN400.152CYT0-0.200913-000iCytoplasmic9.2683.82
GABT_ECOLIP22256gabTb2662 JW2637 4-aminobutyrate aminotransferase (EC 2.6.1.19) ((S)-3-amino-2-methylpropionate transaminase) (EC 2.6.1.22) (GABA aminotransferase) (GABA-AT) (Gamma-amino-N-butyrate transaminase) (GABA transaminase) (Glutamate:succinic semialdehyde transaminase) (L-AIBAT)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN650.16CYT0-0.200913-000iCytoplasmic9.9777.26
ADD_ECOLIP22333addb1623 JW1615 Adenosine deaminase (EC 3.5.4.4) (Adenosine aminohydrolase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN400.118CYT0-0.200913-000iCytoplasmic9.9715.06
MUKB_ECOLIP22523mukBb0924 JW0907 Chromosome partition protein mukB (Structural maintenance of chromosome-related protein)Nucleoid (experimental). Nucleoid (experimental). Nucleoid (experimental). Cell poles. Peripheral inner membrane protein facing the cytoplasm (experimental).[16982609] [20921377] [11886550] [22380631] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasm nucleoidCytoplasmicyesyesN460.13CYT0-0.200913-000iCytoplasmic10
MUKE_ECOLIP22524mukEkicA ycbAb0923 JW0906 Chromosome partition protein mukE (Protein kicA)Nucleoid (experimental). Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[16982609] [20921377] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasm nucleoidCytoplasmicyesyesN250.113CYT0-0.200913-000iCytoplasmic9.9785.77
LIVF_ECOLIP22731livFb3454 JW3419 High-affinity branched-chain amino acid transport ATP-binding protein livF (LIV-I protein F)Peripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN420.111CYT0-0.200913-000iCytoplasmicMembrane7.8815.01
FECI_ECOLIP23484fecIb4293 JW4253 RNA polymerase sigma factor fecIPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Interacts with FecR at the inner membrane propably through the N-terminal region of FecR (1-85) which is cytoplasmic[16718597]. FecR spans the cytoplasmic membrane once, interacts in the periplasm with its C-terminal region with FecA occupied by ferric citrate, and transmits the information through the cytoplasmic membrane into the cytoplasm, where it converts FecI into an active sigma factor[9573190]. FecI specifically binds FecR (a transmembrane protein) and that this binding is necessary for FecI to function as a sigma factor[12057967]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12057967] [16718597] [9573190] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmicnoyesN230.11CYT0-0.200913-000iCytoplasmic1016.07
PPSA_ECOLIP23538ppsAppsb1702 JW1692 Phosphoenolpyruvate synthase (PEP synthase) (EC 2.7.9.2) (Pyruvate, water dikinase)Peripheral inner membrane protein facing the cytoplasm (experimental).One very weak transmembrane region predicted. Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN560.169CYT0-0.200913-000iCytoplasmic9.9748.82
PSS_ECOLIP23830pssApssb2585 JW2569 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) (Phosphatidylserine synthase)Peripheral inner membrane protein facing the cytoplasm (experimental).Phospholipid metabolism complex PlsB-PssA-YbgC-Acp. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][16294310] [19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicTFyesyesN480.108CYT0-0.200913-000iCytoplasmic1035.8
PPIB_ECOLIP23869ppiBb0525 JW0514 Peptidyl-prolyl cis-trans isomerase B (PPIase B) (EC 5.2.1.8) (Rotamase B)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic[23230279] [9268657]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmic (Experimental)yesyesN100.101CYT0-0.200913-000iCytoplasmic1064.37
HEMH_ECOLIP23871hemHpopA visAb0475 JW0464 Ferrochelatase (EC 4.99.1.1) (Heme synthase) (Protoheme ferro-lyase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN630.136CYT0-0.200913-000iCytoplasmic1015.62
FEPC_ECOLIP23878fepCb0588 JW0580 Ferric enterobactin transport ATP-binding protein fepCPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN630.171CYT0-0.200913-000iCytoplasmicMembrane9.5917.69
GSA_ECOLIP23893hemLgsa popCb0154 JW0150 Glutamate-1-semialdehyde 2,1-aminomutase (GSA) (EC 5.4.3.8) (Glutamate-1-semialdehyde aminotransferase) (GSA-AT)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN260.222CYT0-0.200913-000iCytoplasmic9.9733.26
ARGE_ECOLIP23908argEb3957 JW3929 Acetylornithine deacetylase (AO) (Acetylornithinase) (EC 3.5.1.16) (N-acetylornithinase) (NAO)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN230.163CYT0-0.200913-000iCytoplasmic9.9742.16
RFAC_ECOLIP24173rfaCrfa-2 waaC yibCb3621 JW3596 Lipopolysaccharide heptosyltransferase 1 (EC 2.-.-.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN500.195CYT0-0.200913-000iCytoplasmic9.9751.85
ACCC_ECOLIP24182accCfabGb3256 JW3224 Biotin carboxylase (EC 6.3.4.14) (Acetyl-CoA carboxylase subunit A) (ACC) (EC 6.4.1.2)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation [19737520].[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN360.117CYT0-0.200913-000iCytoplasmic9.9729.79
RADA_ECOLIP24554radAsmsb4389 JW4352 DNA repair protein radA (DNA repair protein sms)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN410.199CYT0-0.200913-000iCytoplasmicMembrane9.8229.48
FRMA_ECOLIP25437frmAadhCb0356 JW0347 S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) (Alcohol dehydrogenase class-3) (EC 1.1.1.1) (Alcohol dehydrogenase class-III) (Glutathione-dependent formaldehyde dehydrogenase) (FALDH) (FDH) (GSH-FDH) (EC 1.1.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmCytoplasmicyesyesN500.109CYT0-0.200913-000iCytoplasmic1071.48
HFLX_ECOLIP25519hflXb4173 JW4131 GTP-binding protein hflXPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (experimental).The hflALocus was found to comprise three genes, hflX, hflK, and hflC. HflK and HflC are membrane proteins and form a mem- brane-bound protease complex along with FtsH(HflB), and they are thought to act as modulators of the function of HflB. The role of the HflX GTPase/ATPase is unclear but has been shown to bind to ribosomes. When overexpressed, a portion of HflXProtein appeared in the membrane fraction, while larger amounts fractionated with the cytoplasmic pool (unpublished data cited in [19181811]). In Corynebacterium glutamicum, HflX has been found to occur equally in the cytoplasm and in the membrane fractions ([15978086]). Has no transmembrane region either in E. coli or in C. glutamicum. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19181811] [15978086] [19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN520.116CYT0-0.200913-000iCytoplasmic8.9652.85
MNME_ECOLIP25522mnmEthdF trmEb3706 JW3684 tRNA modification GTPase mnmE (EC 3.6.-.-)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm.A GTPase that exhibits high intrinsic GTPase hydrolysis rate,Low affinity for GTP, and extremely low affinity for GDP Can form self-assemblies. Is a cytoplasmic protein partially associated with the inner membrane. Involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs. Essential for viability depending on genetic background. Fluorescent fusions of MnmE have been monitored via fluorescence microscopy. MnmE located predominantly beside the membrane.[10601028] [PMC1171769] [17272300]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN470.153CYT0-0.200913-000iCytoplasmic9.1289.7
GABD_ECOLIP25526gabDb2661 JW2636 Succinate-semialdehyde dehydrogenase [NADP+] (SSDH) (EC 1.2.1.16)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN280.124CYT0-0.200913-000iCytoplasmic9.9760.21
UBIH_ECOLIP25534ubiHvisBb2907 JW2875 2-octaprenyl-6-methoxyphenol hydroxylase (EC 1.14.13.-)Peripheral inner membrane protein facing the cytoplasm (potential).The biosynthesis of UQ is studied in great detail in bacteria and yeast but only to a limited extent in animal tissues. The UQ biosynthetic enzymes may constitute a complex that is tightly bound to the membrane.[11583838]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN420.38SpI251.25365-00Yn3-13c18/19oUnknown6.4915.42
HTRL_ECOLIP25666htrLyibBb3618 JW5644 Nucleoside permease nupG (Nucleoside-transport system protein nupG)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN450.131CYT0-0.200913-000iCytoplasmic8.9650.03
RFAG_ECOLIP25740rfaGpcsA waaGb3631 JW3606 Lipopolysaccharide core biosynthesis protein rfaG (EC 2.4.-.-) (Glucosyltransferase I)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN250.118CYT0-0.200913-000iCytoplasmic9.9730.27
RFAP_ECOLIP25741rfaPwaaPb3630 JW3605 Lipopolysaccharide core heptose(I) kinase rfaP (EC 2.7.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN200.155CYT0-0.200913-000iCytoplasmic9.9733.61
RFAQ_ECOLIP25742rfaQwaaQb3632 JW3607 Lipopolysaccharide core heptosyltransferase rfaQ (EC 2.-.-.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN250.11CYT0-0.200913-000iCytoplasmic8.9633.16
HFLD_ECOLIP25746hflDycfCb1132 JW5165 High frequency lysogenization protein hflDPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).Downregulates lambda lysogenization;Interacts with cIIProtein; first gene in purB operon Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11278968] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicyesyesN540.157CYT0-0.200913-000iUnknown219.39
RHLE_ECOLIP25888rhlEb0797 JW0781 ATP-dependent RNA helicase rhlE (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmAssembly cofactor of ribosome; associates with the 70S subunit. Overexpression of protein suppresses defects in deletion of DeaD. [18083833][21529161] [23230279] [18083833]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN460.14CYT0-0.200913-000iCytoplasmic9.9762.62
WAAU_ECOLIP27242waaUrfaK waaKb3623 JW3598 IlvGMEDA operon leader peptide (IlvGMEDA operon attenuator peptide)Peripheral inner membrane protein facing the cytoplasm (experimental).[1657881]Peripheral inner membrane protein facing the cytoplasmF1N/AMembrane | Peripheral membrane proteinCytoplasmicnoyesN330.113CYT0-0.200913-000iCytoplasmic8.96
PLSX_ECOLIP27247plsXb1090 JW5156 Phosphate acyltransferase (EC 2.3.1.n2) (Acyl-ACP phosphotransacylase) (Acyl-[acyl-carrier-protein]--phosphate acyltransferase) (Phosphate-acyl-ACP acyltransferase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN240.213CYT0-0.200913-000iCytoplasmic9.2615.13
GCST_ECOLIP27248gcvTb2905 JW2873 Aminomethyltransferase (EC 2.1.2.10) (Glycine cleavage system T protein)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN310.116CYT0-0.200913-000iCytoplasmic9.97101.03
ARGK_ECOLIP27254argKygfDb2918 JW2885 LAO/AO transport system kinase (EC 2.7.-.-)Peripheral inner membrane protein facing the cytoplasmAppears to phosphorylate the periplasmic binding proteins of the lysine, arginine, ornithine transport system[2136858].[2136858]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN260.12CYT0-0.200913-000iCytoplasmicMembrane1023.34
NADR_ECOLIP27278nadRnadIb4390 JW5800 Transcriptional regulator nadRPeripheral inner membrane protein facing the cytoplasm (potential). Nucleoid protein (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).May loosely associate with the membrane through its interaction with the membrane-bound pnuC (Nicotinamide riboside transporter). In combination with corepressor (NAD?), the cytoplasmic form of nadR would be capable of acting as a transcriptional repressor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10464228] [23138451] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/AMembrane | Peripheral membrane proteinCytoplasmCytoplasmicyesyesN390.131CYT0-0.200913-000iCytoplasmic8.9639.49
OPDA_ECOLIP27298prlCopdAb3498 JW3465 Oligopeptidase A (EC 3.4.24.70)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN260.115CYT0-0.200913-000iCytoplasmic1094.24
TKT1_ECOLIP27302tktAtktb2935 JW5478 Transketolase 1 (TK 1) (EC 2.2.1.1)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN250.124CYT0-0.200913-000iCytoplasmic9.2632.23
STHA_ECOLIP27306sthAsth udhAb3962 JW5551 Soluble pyridine nucleotide transhydrogenase (STH) (EC 1.6.1.1) (NAD(P)(+) transhydrogenase [B-specific])Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).SthA is bound on the inner mebrane through PntAB heterodimer. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][14660605] [21545646] [20732298] [19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN220.133CYT0-0.200913-000iCytoplasmic1022.56
ACSA_ECOLIP27550acsyfaCb4069 JW4030 Acetyl-coenzyme A synthetase (EC 6.2.1.1) (Acetate--CoA ligase) (Acyl-activating enzyme)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (experimental).Homologous to FadD13 protein of Mycobacterium that is peripheral. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][22560731] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN270.136CYT0-0.200913-000iCytoplasmic9.9762.33
WECG_ECOLIP27836wecGrffMb3794 JW3770 Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (UDP-ManNAcA transferase) (EC 2.4.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[12618464] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN270.16CYT0-0.200913-000iCytoplasmic8.9635.86
TREC_ECOLIP28904treColgHb4239 JW4198 Trehalose-6-phosphate hydrolase (EC 3.2.1.93) (Alpha,alpha-phosphotrehalase)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFyesyesN600.145CYT0-0.200913-000iCytoplasmic109.49
ALR2_ECOLIP29012dadXalnB dadBb1190 JW1179 Alanine racemase, catabolic (EC 5.1.1.1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN250.206CYT0-0.200913-000iCytoplasmic102.69
METN_ECOLIP30750metNabcb0199 JW0195 Methionine import ATP-binding protein MetN (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN420.139CYT0-0.200913-000iCytoplasmicMembrane9.8223.08
RNB_ECOLIP30850rnbb1286 JW1279 Exoribonuclease 2 (EC 3.1.13.1) (Exoribonuclease II) (RNase II) (Ribonuclease II)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II [23344958] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330][23344958] [24438330]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasmCytoplasmicyesyesN230.181CYT0-0.200913-000iCytoplasmic1048.82
MODF_ECOLIP31060modFphrAb0760 JW0743 Uncharacterized protein yciQPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN340.221CYT0-0.200913-000iCytoplasmicMembrane9.8233.64
POTG_ECOLIP31134potGb0855 JW5818 Putrescine transport ATP-binding protein potGPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN350.11CYT0-0.200913-000iCytoplasmicMembrane7.8811.11
THIQ_ECOLIP31548thiQyabJb0066 JW0065 Thiamine import ATP-binding protein ThiQ (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm.Two very weak TM predictions; membrane associated activity known. Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system [12175925][4889357] [12175925]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN250.144CYT0-0.200913-000iCytoplasmicMembrane108.94
CAIC_ECOLIP31552caiCyaaMb0037 JW0036 Probable crotonobetaine/carnitine-CoA ligase (EC 6.2.1.-)Peripheral inner membrane protein facing the cytoplasm (potential).Homolog to FadD13 protein of Mycobacterium that is peripheral.[22560731]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN180.105CYT0-0.200913-000iCytoplasmic9.97
OTSA_ECOLIP31677otsAb1896 JW5312 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15) (Osmoregulatory trehalose synthesis protein A) (Trehalose-6-phosphate synthase) (UDP-glucose-glucosephosphate glucosyltransferase)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN190.248CYT0-0.200913-000iCytoplasmic1030.22
NUOF_ECOLIP31979nuoFb2284 JW2279 NADH-quinone oxidoreductase subunit F (EC 1.6.99.5) (NADH dehydrogenase I subunit F) (NDH-1 subunit F) (NUO6)Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.cThermus homologue: Q56222 (NQO1_THET8) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN570.101CYT0-0.200913-000iCytoplasmic9.1211.9
TYPA_ECOLIP32132typAbipA yihKb3871 JW5571 GTP-binding protein typA/BipA (Tyrosine phosphorylated protein A)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicTFyesyesN370.112CYT0-0.200913-000iCytoplasmicMembrane7.8888.51
PTFA_ECOLIP32155frvAyiiKb3900 JW3871 Fructose-like phosphotransferase enzyme IIA component (EC 2.7.1.-) (PTS system fructose-like EIIA component)Peripheral inner membrane protein facing the cytoplasm (potential).Fructose-like phosphotransferase enzyme IIA component [7773398]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN190.135CYT0-0.200913-000iCytoplasmic9.2668.52
PTFX2_ECOLIP32670ptsAfrwA yijHb3947 JW5555 Multiphosphoryl transfer protein 2 (MTP 2) [Includes: Phosphoenolpyruvate-protein phosphotransferase (EC 2.7.3.9) (Enzyme I-Ani) (Phosphotransferase system enzyme I); Phosphocarrier protein HPr (Protein H); Fructose-like phosphotransferase enzyme IIA compPeripheral inner membrane protein facing the cytoplasm Multiphosphoryl transfer protein 2 Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN180.56CYT0-0.200913-000iCytoplasmic9.9768.84
PTFB3_ECOLIP32676frwDyijNb3953 JW3925 Fructose-like phosphotransferase enzyme IIB component 3 (EC 2.7.1.69) (PTS system fructose-like EIIB component 3)Peripheral inner membrane protein facing the cytoplasm Sequence analysis indicates that an frwD encoded protein has similarity to the IIB domains of the PTS Enzymes II specific for fructose. [16397293] [17904518] Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN210.559CYT0-0.200913-00Yn4-12c17/18oUnknown6.4943.83
RLUF_ECOLIP32684rluFyjbCb4022 JW3982 Ribosomal large subunit pseudouridine synthase F (EC 5.4.99.-) (rRNA pseudouridylate synthase F) (rRNA-uridine isomerase F)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN250.109CYT0-0.200913-000iCytoplasmic9.97
DUSA_ECOLIP32695dusAyjbNb4049 JW5950 tRNA-dihydrouridine synthase A (EC 1.-.-.-)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN270.108CYT0-0.200913-000iCytoplasmic9.97
ALSA_ECOLIP32721alsAyjcWb4087 JW4048 D-allose import ATP-binding protein AlsA (EC 3.6.3.17)Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN210.291CYT0-0.200913-000iCytoplasmicMembrane1018.1
GCSP_ECOLIP33195gcvPb2903 JW2871 Glycine dehydrogenase [decarboxylating] (EC 1.4.4.2) (Glycine cleavage system P-protein) (Glycine decarboxylase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN600.111CYT0-0.200913-010o751-770iCytoplasmic9.9721.54
LLDD_ECOLIP33232lldDlctDb3605 JW3580 L-lactate dehydrogenase [cytochrome] (EC 1.1.2.3)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Membrane associated Membrane associated Peripheral inner membrane protein facing the cytoplasm (experimental).LldD is a cytochrome. There is a strong experimental indication that it is a membrane bound protein although no transmembrane helix is predicted [18473]. [8407843] [6997721] [18473] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][18473] [15919657] [8407843] [6997721] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)Membrane associated (Experimental)yesyesN320.129CYT0-0.200913-000iCytoplasmic9.264.07
MLRA_ECOLIP33358mlrAyehVb2127 JW2115 HTH-type transcriptional regulator mlrA (MerR-like regulator A)Peripheral inner membrane protein facing the cytoplasm (potential).MlrA has regulatory role in transcription activation of csgD, which is the master regulator of biofilm formation.[20874755]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1IM (predicted)CytoplasmicyesyesN230.15CYT0-0.200913-010o135-155iCytoplasmic9.26114.83
YEHX_ECOLIP33360yehXb2129 JW2117 Putative osmoprotectant uptake system ATP-binding protein yehX (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN420.103CYT0-0.200913-000iCytoplasmicMembrane7.888.73
TKT2_ECOLIP33570tktBb2465 JW2449 Transketolase 2 (TK 2) (EC 2.2.1.1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN240.145CYT0-0.200913-000iCytoplasmic9.9711.23
NIKD_ECOLIP33593nikDb3479 JW3444 Nickel import ATP-binding protein NikD (EC 3.6.3.24)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN480.168CYT0-0.200913-000iCytoplasmicMembrane7.8845.04
NIKE_ECOLIP33594nikEb3480 JW3445 Nickel import ATP-binding protein NikE (EC 3.6.3.24)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN680.149CYT0-0.200913-000iCytoplasmicMembrane9.8241.99
NUOCD_ECOLIP33599nuoCnuoCD nuoDb2286 JW5375 NADH-quinone oxidoreductase subunit C/D (EC 1.6.99.5) (NADH dehydrogenase I subunit C/D) (NDH-1 subunit C/D) (NUO3/NUO4)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [7607227] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19737520] [16079137] [15919657] [21541338] [7607227] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)TFyesyesN390.108CYT0-0.200913-000iCytoplasmic9.9717.69
NUOG_ECOLIP33602nuoGb2283 JW2278 NADH-quinone oxidoreductase subunit G (EC 1.6.99.5) (NADH dehydrogenase I subunit G) (NDH-1 subunit G) (NUO7)Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. [11416161] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][20732298] [19737520] [16079137] [15919657] [11416161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)TFyesyesN570.143CYT0-0.200913-000iCytoplasmicMembrane1017.7
YEJF_ECOLIP33916yejFb2180 JW2168 Uncharacterized ABC transporter ATP-binding protein yejFPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN350.139CYT0-0.200913-000iCytoplasmicMembrane9.99
CCMA_ECOLIP33931ccmAyejWb2201 JW5366 Cytochrome c biogenesis ATP-binding export protein CcmA (EC 3.6.3.41) (Heme exporter protein A)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN290.16CYT0-0.200913-000iCytoplasmicMembrane7.8847.18
MQO_ECOLIP33940mqoyojHb2210 JW2198 Malate:quinone oxidoreductase (EC 1.1.5.4) (MQO) (Malate dehydrogenase [quinone])Peripheral inner membrane protein facing the cytoplasm (experimental). Membrane associated Peripheral inner membrane protein facing the cytoplasm (experimental).High signal PProbability and lipoP SPIPrediction; weak TmHMMPrediction definite membrane association according to literature. MQOIs a flavin adenine dinucleotide (FAD)- and lipid-dependent peripheral membrane protein catalyzing the oxidation ofL-malate to oxaloacetate. [11092847] [368072] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][11092847] [368072] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Membrane associated (Experimental)yesyesY270.278SpI218.41159-00Yn4-15c21/22oUnknown6.4917.8
AHPF_ECOLIP35340ahpFb0606 JW0599 Alkyl hydroperoxide reductase subunit F (EC 1.8.1.-) (Alkyl hydroperoxide reductase F52A protein)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential). Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bridge (345 348) [19766000] [23230279] Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN350.108CYT0-0.200913-000iCytoplasmicMembrane7.8876.12
WBBL_ECOLIP36667wbbLb4571 JW2016 b2031Small toxic protein ibsAPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN510.111CYT0-0.200913-000iUnknown254.68
ZAPD_ECOLIP36680zapDyacFb0102 JW0099 Cell division protein ZapDCytoplasm Peripherally acossiated with the inner membrane, cytoplasmic side Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity. 22505682[22505682] 22505682Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN390.116CYT0-0.200913-000iCytoplasmic8.9680.17
ACNB_ECOLIP36683acnByacI yacJb0118 JW0114 Aconitate hydratase 2 (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic Trigger factor-sensitive aggregation. [10585860] Disulfide bond-containing protein [25664544][19737520] [23230279] [10585860] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmic (Experimental)TFyesyesN420.124CYT0-0.200913-000iCytoplasmic9.9790.34
YADG_ECOLIP36879yadGb0127 JW0123 Uncharacterized ABC transporter ATP-binding protein yadGPeripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN310.246CYT0-0.200913-000iCytoplasmicMembrane7.885.9
YADI_ECOLIP36881yadIb0129 JW0125 Putative phosphotransferase enzyme IIA component yadI (EC 2.7.1.-) (Putative PTS system EIIA component)Peripheral inner membrane protein facing the cytoplasmPutative phosphotransferase enzyme IIA component yadI Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN460.118CYT0-0.200913-000iCytoplasmic8.9617.63
KDPF_ECOLIP36937kdpFb4513 JW0687 Probable 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) (Beta-hydroxybutyryl-CoA dehydrogenase) (BHBD)Inner membrane. Membrane anchoredKdpF, could be identified on high resolution SDS-polyacrylamide gels in addition to the subunits KdpA, KdpB, and KdpC. Furthermore, it could be demonstrated that KdpF remains associated with the purified complex. As determined by mass spectrometry, this pe [9789555][10608856] [21890895] [9789555]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane proteinMembrane Anchored (Experimental)noyesN230.109CYT0-0.200913-10Yn5-16c22/23oCytoplasmicMembrane1029.27
YAGA_ECOLIP37007yagAb0267 JW0260 Uncharacterized protein yagAPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN290.125CYT0-0.200913-000iCytoplasmic8.962.18
FBPC_ECOLIP37009fbpCafuC yagCb0262 JW0254 Fe(3+) ions import ATP-binding protein FbpC (EC 3.6.3.30)Homologous to MalKPeripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicProphage Integrase (EcoGene)yesnoN620.109CYT0-0.200913-000iCytoplasmicMembrane7.8842.31
PT1P_ECOLIP37177ptsPygdF ygdOb2829 JW2797 Phosphoenolpyruvate-protein phosphotransferase ptsP (EC 2.7.3.9) (Enzyme I-Ntr) (Phosphotransferase system, enzyme I)Peripheral inner membrane protein facing the cytoplasmPhosphoenolpyruvate-protein phosphotransferase ptsP Localization to the inner surface of the cytoplasmic membrane by immunoelectron microscopy of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli. [2644649][2644649]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN330.185CYT0-0.200913-000iCytoplasmic9.979.43
PTKB_ECOLIP37188gatBb2093 JW2077 Galactitol-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (EIIB-Gat) (PTS system galactitol-specific EIIB component)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental). Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544][16963640] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesY200.743SpI198.62134-00Yn5-14c19/20oCytoplasmic10
DPPF_ECOLIP37313dppFdppEb3540 JW3509 Dipeptide transport ATP-binding protein dppFPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN530.133CYT0-0.200913-000iCytoplasmicMembrane9.9924.02
LHGO_ECOLIP37339lhgOygaFb2660 JW2635 Uncharacterized protein ygaFPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted), Soluble IM FractionCytoplasmicyesyesN210.14CYT0-0.200913-000iCytoplasmic9.1215.17
DHAM_ECOLIP37349dhaMycgCb1198 JW5185 PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM [Includes: Phosphocarrier protein HPr (Protein H); Phosphotransferase enzyme IIA component (EC 2.7.1.-) (PTS system EIIA component)]Peripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN360.134CYT0-0.200913-000iCytoplasmic9.9774.46
MENE_ECOLIP37353menEb2260 JW2255 2-succinylbenzoate--CoA ligase (EC 6.2.1.26) (o-succinylbenzoyl-CoA synthetase) (OSB-CoA synthetase)Peripheral inner membrane protein facing the cytoplasm (potential).Homologous to FadD13 protein of Mycobacterium that is peripheral.[22560731]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN480.113CYT0-0.200913-000iCytoplasmic9.9731.4
XYLG_ECOLIP37388xylGb3567 JW3539 Xylose import ATP-binding protein XylG (EC 3.6.3.17)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN200.188CYT0-0.200913-000iCytoplasmicMembrane7.8848
RFAF_ECOLIP37692rfaFwaaFb3620 JW3595 ADP-heptose--LPS heptosyltransferase 2 (EC 2.-.-.-) (ADP-heptose--LPS heptosyltransferase II)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN420.134CYT0-0.200913-000iCytoplasmic9.2624.65
YEFG_ECOLIP37749yefGyefGb2034 JW2019 L-fuculose phosphate aldolase (EC 4.1.2.17) (L-fuculose-1-phosphate aldolase)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicnoyesN420.102CYT0-0.200913-000iCytoplasmic8.9614.63
WBBJ_ECOLIP37750wbbJyefHb2033 JW2018 Putative lipopolysaccharide biosynthesis O-acetyl transferase wbbJ (EC 2.3.1.-)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN240.107CYT0-0.200913-000iCytoplasmic9.26
WBBK_ECOLIP37751yefIyefIb2032 JW2017 Uncharacterized protein yefIPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544][23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN210.119CYT0-0.200913-000iUnknown232.03
RLUB_ECOLIP37765rluByciLb1269 JW1261 Ribosomal large subunit pseudouridine synthase B (EC 5.4.99.-) (rRNA pseudouridylate synthase B) (rRNA-uridine isomerase B)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN320.101CYT0-0.200913-000iCytoplasmic9.97
YECC_ECOLIP37774yecCb1917 JW1902 Uncharacterized amino-acid ABC transporter ATP-binding protein yecCPeripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN340.123CYT0-0.200913-000iCytoplasmicMembrane7.8837.01
FADK_ECOLIP38135fadKydiDb1701 JW5910 Short-chain-fatty-acid--CoA ligase (EC 6.2.1.-) (Acyl-CoA synthetase)Peripheral inner membrane protein facing the cytoplasm (potential).Homologous to FadD13 protein of Mycobacterium that is peripheral[22560731]Peripheral inner membrane protein facing the cytoplasmF1N/AMembrane | Peripheral membrane proteinCytoplasmicnoyesN370.131CYT0-0.200913-000iCytoplasmic9.97
NFNB_ECOLIP38489nfnBdprA nfsB nfsI ntrb0578 JW0567 Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) (Dihydropteridine reductase) (EC 1.5.1.34) (FMN-dependent nitroreductase)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic[23230279] [12788749]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cytoplasmic (Experimental)yesyesN180.166CYT0-0.200913-000iUnknown286.89
USPG_ECOLIP39177uspGybdQ yzzUb0607 JW0600 Universal stress protein GPeripheral inner membrane protein facing the cytoplasm (potential). Cytoplasmic Cytoplasmic Cytoplasmic Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [8740179] [9600841] [12071968] [16460009] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [8740179] [9600841] [12071968] [16460009] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmic (Experimental)yesyesN530.335CYT0-0.200913-000iCytoplasmic8.9685.6
RSGA_ECOLIP39286rsgAengC yjeQb4161 JW4122 Putative ribosome biogenesis GTPase RsgA (EC 3.6.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).Assembly cofactor of ribosome; associate with the 30S subunit.[21529161] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN530.108CYT0-0.200913-000iCytoplasmic10
YJFM_ECOLIP39295yjfMb4185 JW4143 Uncharacterized protein yjfMPeripheral inner membrane protein facing the cytoplasm.YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC.[20545840]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicnoyesN550.107CYT0-0.200913-000iUnknown2105.05
YJGR_ECOLIP39342yjgRb4263 JW4220 Uncharacterized protein yjgRPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN580.139CYT0-0.200913-000iUnknown287.21
RSMC_ECOLIP39406rsmCyjjTb4371 JW4333 Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52) (16S rRNA m2G1207 methyltransferase) (rRNA (guanine-N(2)-)-methyltransferase rsmC)Peripheral inner membrane protein facing the cytoplasm (experimental).Ribosom related[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN270.158CYT0-0.200913-000iCytoplasmic9.2643.09
ADHP_ECOLIP39451adhPyddNb1478 JW1474 Alcohol dehydrogenase, propanol-preferring (EC 1.1.1.1)Peripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicyesyesN400.124CYT0-0.200913-000iCytoplasmic9.9786.56
MTOX_ECOLIP40874solAb1059 JW1046 N-methyl-L-tryptophan oxidase (MTOX) (EC 1.5.3.-)Peripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicyesyesN160.149CYT0-0.200913-000iCytoplasmic9.9773.12
AZOR_ECOLIP41407azoRacpDb1412 JW1409 FMN-dependent NADH-azoreductase (EC 1.7.-.-) (Azo-dye reductase) (FMN-dependent NADH-azo compound oxidoreductase)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN190.135CYT0-0.200913-00Yn4-13c18/19oCytoplasmic9.26108.78
UXAA_ECOLIP42604uxaAygjWb3091 JW3062 Altronate hydrolase (EC 4.2.1.7) (Altronic acid hydratase)Peripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicyesyesN680.185CYT0-0.200913-000iCytoplasmic8.96113.96
OBG_ECOLIP42641obgEcgtA obg yhbZb3183 JW3150 GTPase ObgE/CgtA (GTP-binding protein Obg)Ribosome CytoplasmAssembly cofactor of ribosome; associates 30S, 50S,16S, and 23S rRNA. [15292126] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][21529161] [15292126] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN250.112CYT0-0.200913-000iCytoplasmic8.9693.93
HRPA_ECOLIP43329hrpAb1413 JW5905 ATP-dependent RNA helicase hrpA (EC 3.6.4.13)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN520.107CYT0-0.200913-000iCytoplasmic8.96
YCAK_ECOLIP43340ycaKb0901 JW0884 Uncharacterized NAD(P)H oxidoreductase ycaK (EC 1.6.99.-)Peripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicnoyesN230.238CYT0-0.200913-000iCytoplasmic9.9795.76
LPXH_ECOLIP43341lpxHybbFb0524 JW0513 UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).Phobius weak prediction of a hydrophobic TMIn the middle of sequence/Kyte-Doolittle predicts several hydrophobic patches [20608695] [19275539]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN330.16CYT0-0.200913-000iCytoplasmic1020.56
UUP_ECOLIP43672uupycbH ycbIb0949 JW0932 ABC transporter ATP-binding protein uupPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN270.126CYT0-0.200913-000iCytoplasmic10
YRAR_ECOLIP45469yraRb3152 JW5528 Uncharacterized protein yraRPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN320.195CYT0-0.200913-000iCytoplasmic9.2612.27
PROQ_ECOLIP45577proQyebJ yobE yoeCb1831 JW5300 ProP effectorPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN270.162CYT0-0.200913-000iCytoplasmic8.9669.85
LUXS_ECOLIP45578luxSygaGb2687 JW2662 S-ribosylhomocysteine lyase (EC 4.4.1.21) (AI-2 synthesis protein) (Autoinducer-2 production protein luxS)Peripheral inner membrane protein facing the cytoplasm (experimental). Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein [25664544][19737520] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFnoyesN190.106CYT0-0.200913-000iCytoplasmic8.96110.02
GSPE_ECOLIP45759gspEyheGb3326 JW3288 Probable general secretion pathway protein E (Type II traffic warden ATPase)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm.ATPase of the Type II secretion system (cryptic in K12) EspL (GspL homolog) protein interacts through its N-terminal cytoplasmic domain with EspL (GspL homolog) protein[18175178] [17255937] [16525507] [15093830] [11118204] [PMC398259]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN230.111CYT0-0.200913-000iCytoplasmic9.9721.54
YHDZ_ECOLIP45769yhdZb3271 JW3239 Uncharacterized amino-acid ABC transporter ATP-binding protein yhdZPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN120.122CYT0-0.200913-000iCytoplasmicMembrane9.9911.37
YEDJ_ECOLIP46144yedJb1962 JW1945 Uncharacterized protein yedJPeripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicnoyesN650.139CYT0-0.200913-000iCytoplasmic8.96116.91
YHGF_ECOLIP46837yhgFb3407 JW3370 Protein yhgFPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN240.217CYT0-0.200913-000iCytoplasmic9.9790.52
MLC_ECOLIP50456mlcb1594 JW1586 Protein mlc (Making large colonies protein)Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Cytoplasmic global repressor of gene transcription that is recruited to the inner membrane where it binds the PtsB protein after the latter has been phosphorylated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15255904] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1IM (predicted)CytoplasmicyesyesN430.124CYT0-0.200913-000iCytoplasmic9.9718.29
AEEP_ECOLIP51981ycjGycjHb1325 JW1318 L-Ala-D/L-Glu epimerase (AE epimerase) (AEE) (EC 5.1.1.-)Peripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicSECyesyesN210.226CYT0-0.200913-000iCytoplasmic8.9619.53
CSGD_ECOLIP52106csgDb1040 JW1023 CsgBAC operon transcriptional regulatory proteinNucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Nucleoid (experimental). NucleoidNecessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. CsgD despite its function as a transcription activator, is localized in the cytoplasmic membrane. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054). [20874755] [16513732] [23138451] [21645131] [19131513] Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN260.267CYT0-0.200913-000iCytoplasmic9.2620.1
YFJP_ECOLIP52131yfjPb2632 JW5419 Uncharacterized protein yfjPPeripheral inner membrane protein facing the cytoplasm.Fluorescent fusions of YfjP have been monitored via fluorescence microscopy. YfjP located predominantly beside the membrane.[17272300]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicProphage Integrase (EcoGene)nonoN590.126CYT0-0.200913-000iUnknown235.33
WECF_ECOLIP56258wecFrffT yifMb4481 JW5596 4-alpha-L-fucosyltransferase (EC 2.4.1.-) (TDP-Fuc4NAc:lipid II Fuc4NAc transferase) (Fuc4NAc transferase)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)Cell inner membrane | Peripheral membrane proteinCytoplasmicpseudogene (Ochman & Davalos, 2006)yesyesN290.203CYT0-0.200913-000iUnknown248.35in/in
SGCB_ECOLIP58035sgcBb4565 JW5967 Putative phosphotransferase enzyme IIB component sgcB (EC 2.7.1.69) (Putative PTS system EIIB component)Peripheral inner membrane protein facing the cytoplasmPart of SgcABC complex, a putative PTSPermease. SgcA (Enzyme IIASgc)Is homologous to the IIA domains of the fructose - and mannitol-specific PTSPermeases (fruB and cmtB) which are peripherally accossiated proteins. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN190.218SpI214.43024-00Yn4-11c19/20oCytoplasmic9.26
MUKF_ECOLIP60293mukFkicBb0922 JW0905 Chromosome partition protein mukF (Protein kicB)Nucleoid (experimental). Nucleoid (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).[16982609] [20921377] [23230279]Nucleoid, Peripheral inner membrane protein facing the cytoplasmN, F1N/ACytoplasm nucleoidCytoplasmicyesyesN370.127CYT0-0.200913-000iCytoplasmic1076.09
RSMH_ECOLIP60390rsmHmraW yabCb0082 JW0080 Ribosomal RNA small subunit methyltransferase H (EC 2.1.1.-) (16S rRNA m(4)C1402 methyltransferase) (rRNA (cytosine-N(4)-)-methyltransferase RsmH)Peripheral inner membrane protein facing the cytoplasm (potential). Cytoplasm Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [10572301] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [10572301] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN540.11CYT0-0.200913-000iCytoplasmic1065.51
HIS1_ECOLIP60757hisGb2019 JW2001 ATP phosphoribosyltransferase (ATP-PRT) (ATP-PRTase) (EC 2.4.2.17)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN440.181CYT0-0.200913-000iCytoplasmic1064.44
LEPA_ECOLIP60785lepAb2569 JW2553 GTP-binding protein lepAPeripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental).LepA has an amino-terminal domain of 112 residues that is homologous to a domain of similar size found in initiation factor 2, elongation factor Tu, and elongation factor G (IF2, EF-Tu, and EF-G). GTP-photoaffinity labeling and subcellular fractionation experiments indicate that LepA a membrane-bound GTP-binding protein [2999765]. GTPase, EF4/LepA involved in translational elongation, EF4 is stored at the membrane of Escherichia coli cells and released into the cytoplasm upon conditions of high ionic strength or low temperature where EF4 binds to stalled ribosomes and remobilizes them[21300907].Trigger factor-sensitive aggregation[19737520]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][2999765] [21300907] [19737520] [15919657] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell membranePeriplasmCytoplasmicTFyesyesN390.117CYT0-0.200913-000iCytoplasmicMembrane1045.96
SYH_ECOLIP60906hisSb2514 JW2498 Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN200.103CYT0-0.200913-000iCytoplasmic1059.15
RISB_ECOLIP61714ribHribH ybaFb0415 JW0405 6,7-dimethyl-8-ribityllumazine synthase (DMRL synthase) (Lumazine synthase) (EC 2.5.1.9) (Riboflavin synthase beta chain)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN690.129CYT0-0.200913-000iCytoplasmic9.26
MDH_ECOLIP61889mdhb3236 JW3205 Malate dehydrogenase (EC 1.1.1.37)Peripheral inner membrane protein facing the cytoplasm (potential). Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation. [7028159] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][15976458] [19737520] [7028159] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmic (Experimental)TFyesyesN180.251SpI170.386899-00Yn3-12c17/18oUnknown6.49
ISPG_ECOLIP62620ispGgcpEb2515 JW2499 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (EC 1.17.7.1) (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase) (Protein gcpE) (Protein E)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN440.118CYT0-0.200913-000iCytoplasmic9.97
GPMA_ECOLIP62707gpmAgpm pgm pgmAb0755 JW0738 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (BPG-dependent PGAM) (PGAM) (Phosphoglyceromutase) (dPGM) (EC 5.4.2.1)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN410.153CYT0-0.200913-000iCytoplasmic8.96102.79
CLPB_ECOLIP63284clpBhtpMb2592 JW2573 Chaperone protein ClpB (Heat shock protein F84.1)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm[23230279] [8376377]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN220.119CYT0-0.200913-000iCytoplasmic1075.38
MLAF_ECOLIP63386mlaFyrbFb3195 JW3162 Probable phospholipid import ATP-binding protein mlaF (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm (experimental).Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane protein | Cytoplasmic sideCytoplasmicyesyesN640.109CYT0-0.200913-000iCytoplasmicMembrane1023.46
YHES_ECOLIP63389yheSb3352 JW3315 Uncharacterized ABC transporter ATP-binding protein yheSPeripheral inner membrane protein facing the cytoplasm (potential).Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding proteins Trigger factor-sensitive aggregation.[19737520]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN540.115CYT0-0.200913-000iCytoplasmic9.9739.28
MLAB_ECOLIP64602mlaByrbBb3191 JW5535 Probable phospholipid ABC transporter-binding protein mlaBPeripheral inner membrane protein facing the cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental).Probable phospholipid ABC transporter-binding protein mlaB Probable phospholipid ABC transporter-binding protein mlaB Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicnoyesN660.108CYT0-0.200913-000iUnknown2
HLDD_ECOLIP67910hldDhtrM rfaD waaDb3619 JW3594 ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20) (ADP-L-glycero-beta-D-manno-heptose-6-epimerase) (ADP-glyceromanno-heptose 6-epimerase) (ADP-hep 6-epimerase) (AGME)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasmic[23230279] [2198271]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmic (Experimental)yesyesN190.195CYT0-0.200913-000iCytoplasmic9.9726.48
GRCA_ECOLIP68066grcAyfiDb2579 JW2563 Autonomous glycyl radical cofactorPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN300.139CYT0-0.200913-000iCytoplasmic9.97
MALK_ECOLIP68187malKb4035 JW3995 Maltose/maltodextrin import ATP-binding protein MalK (EC 3.6.3.19)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). ATPase of maltose uptake system (potential) Crystal structure of a catalytic intermediate of the maltose transporter. [18033289][16079137] [18456666] [18033289]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN150.106CYT0-0.200913-000iCytoplasmicMembrane1019.25
SRA_ECOLIP68191srarpsVb1480 JW5962 Stationary-phase-induced ribosome-associated protein (Protein D) (SRA) (30S ribosomal protein S22)Ribosome-associated. Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (experimental).Small protein identified experimentally[19121005]. Stationary-phase-induced and ribosome-associated protein[11292794]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry)[23230279].[19121005] [11292794] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN260.1CYT0-0.200913-000iCytoplasmic10
FIXC_ECOLIP68644fixCyaaSb0043 JW0042 Protein fixCIntegral inner membrane Peripheral inner membrane protein. Peripheral inner membrane protein.The protein has hydrophobic patches all over it's sequence. Could be part of an electron transfer system required for anaerobic carnitine reduction so this is consistent with a membrane association. Strong Prediction on both Phobius and TMHMM for an aminoterminal weakly hydrophobic region. Other studies propose that this sequence may be an aminoterminal signal peptide without evidence for cleavage. The fixABCX genes in Rhodospirillum Rubrum encode a putative membrane complex participating in electron transfer to nitrogenase where FixA and B are soluble proteins.However the RrFixC expressed in E.coli was localized at the membrane fraction and was not carbonate extracted suggesting that it is an integral membrane protein. From another study in Rhizobia. Primary sequence homology, the FixC family is a highly conserved flavoprotein distantly related to the mitochondrial ETF-QO (peripheral, membrane-associated ETF : ubiquinone oxidoreductase) yet lacking both hydrophobic and amphipathic transmembrane {alpha}-helices. Thus FixC family is unlikely to be stably membrane-associated.[7473063] [15028689] [14702404]Peripheral inner membrane protein facing the cytoplasmF1N/APeriplasmicyesyesN260.179CYT0-0.200913-00Yn8-19c25/26oCytoplasmic8.9635.51
AMPA_ECOLIP68767pepAcarP xerBb4260 JW4217 Cytosol aminopeptidase (EC 3.4.11.1) (Aminopeptidase A/I) (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN350.112CYT0-0.200913-000iCytoplasmic9.9758.35
KAD_ECOLIP69441adkdnaW plsAb0474 JW0463 Adenylate kinase (AK) (EC 2.7.4.3) (ATP-AMP transphosphorylase)Cytoplasm Peripheral inner membrane protein facing the cytoplasm (experimental). CytoplasmicAdk cell localization has been monitored via fluorescence microscopy after protein was chromosomally tagged with fluorescent protein/s. These experiments indicate that Adk is distributed diffusely throughout cytoplasm. [8650159][17272300] [23230279] [8650159]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmic (Experimental)yesyesN180.12CYT0-0.200913-00Yn3-12c17/18oCytoplasmic10104.67
LCFA_ECOLIP69451fadDoldDb1805 JW1794 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (Long-chain acyl-CoA synthetase) (Acyl-CoA synthetase)Peripheral inner membrane protein facing the cytoplasm (experimental).Acyl-coenzyme A (CoA) synthetase that may interact with the inner membrane. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1460045] [8107670] [20622065] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/AMembrane | Peripheral membrane proteinCytoplasmicyesyesN630.12CYT0-0.200913-000iCytoplasmic9.9736.54
RCMNS_ECOLIP69506ytfEb4209 JW4167 Regulator of cell morphogenesis and NO signaling (RCMNS)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmicyesyesN210.13CYT0-0.200913-000iCytoplasmic10103.79
PTGA_ECOLIP69783crrgsr iex tgs treDb2417 JW2410 Glucose-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (EIIA-Glc) (EIII-Glc) (PTS system glucose-specific EIIA component)Peripheral inner membrane protein facing the cytoplasm (experimental) Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][12717030] [2960675] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmic (Experimental)yesyesN440.106CYT0-0.200913-000iCytoplasmic10113.63
PTQA_ECOLIP69791chbAcelCb1736 JW1725 N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (EIIA-Chb) (EIII-Chb) (PTS system N,N'-diacetylchitobiose-specific EIIA component)Peripheral inner membrane protein facing the cytoplasm (potential) IIA component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC.[10913122]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicpseudogene (Ochman & Davalos, 2006)yesyesN380.187CYT0-0.200913-000iCytoplasmic10111.8
PTQB_ECOLIP69795chbBcelAb1738 JW1727 N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (PTS system N,N'-diacetylchitobiose-specific EIIB component)Peripheral inner membrane protein facing the cytoplasm (potential) Membrane associatedIIB component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC. [2092358][10913122] [2092358]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmMembrane associated (Experimental)yesyesN280.176CYT0-0.200913-00Yn8-19c27/28oCytoplasmic1097.68
PTNAB_ECOLIP69797manXgptB ptsLb1817 JW1806 PTS system mannose-specific EIIAB component (EIIAB-Man) [Includes: Mannose-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (EIII-Man) (PTS system mannose-specific EIIA component); Mannose-specific phosphotransferase enzyme IIB component (EC Peripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental). Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental).N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component. Identified by native-PAGE technique for the study of membrane protein complexes in the inner and outer membranes of Escherichia coli. The mannose phosphotransferase system transporter (EIIman) is composed of three subunits, ManX (IIABman), ManY (IICman), and ManZ (IIDman), which are reported to assemble in a 2:1:2 stoichiometry. The IIC and IID components are integral inner membrane proteins. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [10094700] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][10913122] [16079137] [15919657] [15911532] [10094700] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)noyesN430.126CYT0-0.200913-000iCytoplasmic1093.82
PTFAH_ECOLIP69811fruBfpr fruFb2169 JW2156 Multiphosphoryl transfer protein (MTP) (Phosphotransferase FPr protein) (Pseudo-HPr) [Includes: Phosphocarrier protein HPr (Protein H); Fructose-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (PTS system fructose-specific EIIA component)]Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Cytoplasmic Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [8013873] [2841594][15911532] [8013873] [2841594]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmCytoplasmic (Experimental)noyesN390.108CYT0-0.200913-000iCytoplasmic1091.47
PTFB2_ECOLIP69816frwByijKb3950 JW3922 Fructose-like phosphotransferase enzyme IIB component 2 (EC 2.7.1.69) (PTS system fructose-like EIIB component 2)Peripheral inner membrane protein facing the cytoplasm (potential).The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively. SgaT represents a novel prototypical enzyme IIC that functions with SgaA and SgaB to allow phosphoryl transfer from HPr(his-P) to l-ascorbate via the phosphoryl transfer pathway[8031825]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN210.363SpI204.62655-000iCytoplasmic1087.8
ULAC_ECOLIP69820ulaCptxA sgaA yjfUb4195 JW4153 Ascorbate-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (PTS system ascorbate-specific EIIA component)Peripheral inner membrane protein facing the cytoplasmPeripheral inner membrane protein facing the cytoplasm.[12644495]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN470.126CYT0-0.200913-000iCytoplasmicMembrane7.8884.35
ULAB_ECOLIP69822ulaBsgaB yjfTb4194 JW4152 Ascorbate-specific phosphotransferase enzyme IIB component (EC 2.7.1.69) (Ascorbate-specific PTS system EIIB component)Peripheral inner membrane protein facing the cytoplasm (potential) The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively[12644495]. All three proteins are required for the anaerobic utilization and uptake of L-ascorbate in vivo and its phosphoenolpyruvate-dependent phosphorylation in vitro[12644495].[12644495]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN620.14CYT0-0.200913-000iUnknown253.44
PTMA_ECOLIP69824cmtBb2934 JW2901 Mannitol-specific cryptic phosphotransferase enzyme IIA component (EC 2.7.1.-) (EIIA-Mtl) (EIII-Mtl) (PTS system mannitol-specific EIIA component)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).[9525892] [9260961]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN190.136CYT0-0.200913-000iUnknown296.87
PTKA_ECOLIP69828gatAb2094 JW2078/JW2081 Galactitol-specific phosphotransferase enzyme IIA component (EC 2.7.1.-) (EIIA-Gat) (PTS system galactitol-specific EIIA component)Peripheral inner membrane protein facing the cytoplasm (potential).Part of phosphoenolpyruvate-dependent carbohydrate transport system (PTS). The carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. GatA (IIA domain) interacts with GatB (IIB domain) [16963640] . Copurifies with trigger factor in pull down assays [19737520].[16963640] [19737520]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicTFnoyesN690.109CYT0-0.200913-000iCytoplasmic1071.98
PTSN_ECOLIP69829ptsNrpoP yhbIb3204 JW3171 Nitrogen regulatory protein (EC 2.7.1.-) (Enzyme IIA-NTR) (PTS system EIIA component) (Phosphotransferase enzyme IIA component)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (experimental).enzyme IIA(Ntr) of the nitrogen PTS regulates the intracellular concentration of K(+) by direct interaction with TrkA and KdpD. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][17289841] [21605551] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN260.129CYT0-0.200913-000iCytoplasmic1082.86
DMSD_ECOLIP69853dmsDynfIb1591 JW5262 Twin-arginine leader-binding protein dmsDPeripheral inner membrane protein facing the cytoplasm (experimental). Chaperone for TAT signal peptides The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. ynfFGH are expressed anaerobically. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. YnfH can function with DmsAB, replacing DmsC as the anchor subunit. The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.[14522592] DmsD was confirmed to interact with the general molecular chaperones DnaK, DnaJ, GrpE, GroEL, Tig and Ef-Tu via in vitro protein-protein interaction screening approaches, including affinity chromatography, co-precipitation, and cross-linking. [20153451] DmsD is a redox enzyme maturation protein (REMP) which interacts with the twin-arginine leader sequence of DmsA, the catalytic subunit of DMSO reductase. It interacts with several general chaperones (DnaK, DnaJ, GrpE, GroEL, Tig and Ef-Tu) via in vitro protein-protein interaction screening approaches. It was also found to interact with proteins involved in the molybdenum cofactor biosynthesis pathway. [20153451][19652330] [12813051] [14522592] [20153451]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmCytoplasmicyesyesN310.453SpI302.43679-000iCytoplasmicMembrane10
POTA_ECOLIP69874potAb1126 JW1112 Spermidine/putrescine import ATP-binding protein PotA (EC 3.6.3.31)Peripheral inner membrane protein facing the cytoplasm (experimental). Peripheral inner membrane protein facing the cytoplasm (experimental). Integral Membrane Protein Integral Membrane Protein Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][1939142] [7592703] [11976340] [8366082] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN220.124CYT0-0.200913-000iCytoplasmicMembrane9.9993.56
FCTA_ECOLIP69902frcyfdWb2374 JW2371 Formyl-coenzyme A transferase (Formyl-CoA transferase) (EC 2.8.3.16)Peripheral inner membrane protein facing the cytoplasm (potential).Hypothesized to interact with the oxalate/formate antiporter and to oxalyl-CoA decarboxylase to form a large complex. 3D structure formyl-CoA transferase in Oxalobacter formigenes reveals a CoA binding site at the interface of an interlocked dimer[12839984].[12839984]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN240.119CYT0-0.200913-000iCytoplasmic9.9781.92
DCEB_ECOLIP69910gadBb1493 JW1488 Glutamate decarboxylase beta (GAD-beta) (EC 4.1.1.15)Cytoplasm and Membrane.Localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls.[12912902] [20558177]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmMembraneCytoplasmicyesyesN280.112CYT0-0.200913-000iCytoplasmic1014.81
HDA_ECOLIP69931hdaidaB yfgEb2496 JW5397 DnaA-homolog protein hdaPeripheral inner membrane protein facing the cytoplasm (experimental).Homolgous to DnaA and more specific to C-terminal anchor of DnaA.[12618445]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN330.157CYT0-0.200913-000iCytoplasmic9.97
YAFS_ECOLIP75672yafSb0213 JW0203 Uncharacterized protein yafSPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN540.121CYT0-0.200913-000iCytoplasmic8.96
YAHK_ECOLIP75691yahKb0325 JW0317 Uncharacterized zinc-type alcohol dehydrogenase-like protein yahK (EC 1.-.-.-)Peripheral Inner membrane protein facing the cytoplasm (potential) Peripheral inner membrane protein facing the cytoplasm (experimental). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544] [23230279] [25664544]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicnoyesN530.148CYT0-0.200913-000iCytoplasmic9.9773.5
GSIA_ECOLIP75796gsiAyliAb0829 JW5897 Glutathione import ATP-binding protein gsiA (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN620.122CYT0-0.200913-000iCytoplasmicMembrane9.99
YBJS_ECOLIP75821ybjSb0868 JW5819 Uncharacterized protein ybjSPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN300.118CYT0-0.200913-000iCytoplasmic8.966.62
YBJX_ECOLIP75829ybjXb0877 JW0861 Uncharacterized protein ybjXPeripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).In Shigella the homologous protein is required for the expression, or correct membrane localisation of IcsA (VirG) on the bacterial cell surface.In Pasteurella haemolytica lapB (P32181), which is thought to be membrane-associated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][19766000] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN200.111CYT0-0.200913-000iUnknown221.42
MACA_ECOLIP75830macAybjYb0878 JW0862 Macrolide-specific efflux protein macAPeripheral inner membrane protein facing the cytoplasm. Integral inner membrane. Cell inner membrane, Periplasmic side Cell inner membrane, Periplasmic sideThe crystal structure of MacA has been determined at 3.0A resolution. The inner membrane transporter MacB requires the outer membrane factor TolC and the periplasmic adaptor protein MacA to form a functional tripartite complex. Electron microscopic indicates that the MacA hexamer interacts with TolCIn a tip-to-tip manner. [11544226] [17214741][19254725] [21325274] [21890895] [11544226] [17214741]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinMembrane anchoredyesyesN320.134SpI313.90027-110i12-29oCytoplasmicMembrane7.8845.19
LONH_ECOLIP75867ycbZb0955 JW0938 Putative Lon protease homolog (EC 3.4.21.-) (ATP-dependent protease La homolog)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN240.113CYT0-0.200913-000iCytoplasmic9.97
RLMI_ECOLIP75876rlmIyccWb0967 JW5898 Ribosomal RNA large subunit methyltransferase I (EC 2.1.1.-) (23S rRNA m5C1962 methyltransferase) (rRNA (cytosine-C(5)-)-methyltransferase rlmI)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN290.117CYT0-0.200913-000iCytoplasmic9.97
LOLD_ECOLIP75957lolDycfVb1117 JW5162 Lipoprotein-releasing system ATP-binding protein LolD (EC 3.6.3.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[15276320] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN650.122CYT0-0.200913-000iCytoplasmicMembrane1025.96
YMFJ_ECOLIP75973ymfJb1144 JW1130 Uncharacterized protein ymfJPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN480.139CYT0-0.200913-000iCytoplasmic8.9647.4
YCGR_ECOLIP76010ycgRb1194 JW1183 Flagellar brake protein YcgR (Cyclic di-GMP binding protein YcgR)Peripheral inner membrane protein facing the cytoplasm Bacterial flagellum basal body.Acts as a "backstop brake" to control flagellar motor direction and speed to affect chemotaxis.[20346719] [19121005] [20303158]Peripheral inner membrane protein facing the cytoplasmF1N/ABacterial flagellum basal bodyCytoplasmicflagellumnoyesN600.155CYT0-0.200913-000iCytoplasmic8.9638.72
OPPD_ECOLIP76027oppDb1246 JW1238 Oligopeptide transport ATP-binding protein oppDPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm. Cytoplasmic Peripheral inner membrane protein facing the cytoplasm (experimental). Oligopeptide permease (Opp) transporters are located in the plasma membrane and have functions generally related to the uptake of peptides from the extracellular environment. OppD is the ATP-binding protein. [24793159] Based on sequence similarity, OppB and OppC are the membrane components of the ABC transporter, and OppD and OppF are the ATP-binding components of the ABC transporter [1738314] Opp-LacZ hybrid proteins were used to show that OppB, OppC and OppD are membrane-associated proteins. [2821267] [16045610] [22087229 ] [3926486] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][24793159] [1738314] [2821267] [16045610] [22087229] [3926486] [23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmic (Experimental)yesyesN690.132CYT0-0.200913-000iCytoplasmicMembrane1018.48
YCJR_ECOLIP76044ycjRb1314 JW5202 Uncharacterized protein ycjRPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN470.118CYT0-0.200913-000iCytoplasmic8.9644.1
INSH5_ECOLIP76071insH5b1370 JW1363 Transposase insH for insertion sequence element IS5YPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicInsertion Sequence, TransposasenonoN490.125CYT0-0.200913-000iCytoplasmic8.96
PAAC_ECOLIP76079paaCydbPb1390 JW1385 Outer membrane protein C (Outer membrane protein 1B) (Porin ompC)Peripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicnoyesN570.144CYT0-0.200913-000iCytoplasmic8.96
SUFE_ECOLIP76194sufEynhAb1679 JW1669 Cysteine desulfuration protein sufEPeripheral inner membrane protein. SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction .[12941942]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN660.103CYT0-0.200913-000iCytoplasmic9.26104.06
MTFA_ECOLIP76346mtfAyeeIb1976 JW1958 Protein mtfA (Mlc titration factor A)Peripheral inner membrane protein. CytoplasmCytoplasmic transciption factor that shuttles from chromosome to the membranes as a peripheral protein on the PtsG transporter. Specifically it binds to the unphosphorylated, trnasport-mediating form of the EIIBCGlc subunit. [16855233][15063852] [16855233]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN360.109CYT0-0.200913-000iCytoplasmic8.9687.45
YEEL_ECOLIP76349yeeLb4497 b1979/b1980Putative uncharacterized protein yeeLPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN190.131CYT0-0.200913-000iCytoplasmic8.9613.89
ARND_ECOLIP76472arnDpmrJ yfbHb2256 JW2250 Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (EC 3.5.1.n3)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN670.132CYT0-0.200913-000iCytoplasmic9.9710.61
SIXA_ECOLIP76502sixAyfcWb2340 JW2337 Phosphohistidine phosphatase sixA (EC 3.1.3.-) (RX6)Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential). Peripheral inner membrane protein facing the cytoplasm (potential).Exhibits phosphohistidine phosphatase activity towards the HPt domain of the ArcB sensor involved in the multistep His-Asp phosphorelay[9489669]. The structure of SixA reveals it forms a relatively shallow groove important for accommodating the kidney-shaped four-helix bundle of the HPt domain of AcrB[17628145].[9489669] [17628145] [10734219]Peripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN570.122CYT0-0.200913-000iUnknown279.37
FADI_ECOLIP76503fadIyfcYb2342 JW2339 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (ACSs) (Acetyl-CoA acyltransferase) (Acyl-CoA ligase) (Beta-ketothiolase) (Fatty acid oxidation complex subunit beta)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN320.192CYT0-0.200913-000iCytoplasmic9.979.12
YFFO_ECOLIP76546yffOb2446 Uncharacterized protein yffOPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN610.105CYT0-0.200913-000iCytoplasmic8.96
YFFR_ECOLIP76549yffRb2449 Uncharacterized protein yffRPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicProphage Integrase (EcoGene)nonoN200.169CYT0-0.200913-00Yn3-14c19/20oUnknown2
MAO2_ECOLIP76558maeBypfFb2463 JW2447 NADP-dependent malic enzyme (NADP-ME) (EC 1.1.1.40)Peripheral inner membrane protein facing the cytoplasm (experimental).Trigger factor-sensitive aggregation.[19737520] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicTFyesyesN450.165CYT0-0.200913-000iCytoplasmic9.9781.28
YGEH_ECOLIP76639ygeHb2852 JW2820 Putative cyclic di-GMP phosphodiesterase YliE (EC 3.1.4.52)Peripheral inner membrane protein facing the cytoplasm.Fluorescent fusions of YgeH have been monitored via fluorescence microscopy. YgeH located predominantly beside the membrane.[17272300]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN530.108CYT0-0.200913-000iCytoplasmic8.9619.97
YNJD_ECOLIP76909ynjDb1756 JW5286 Uncharacterized ABC transporter ATP-binding protein ynjDPeripheral inner membrane protein facing the cytoplasm.Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN530.159CYT0-0.200913-000iCytoplasmicMembrane9.8219.97
YLBE_ECOLIP77129ylbEb4572 b0519Uncharacterized protein ylbEPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicpseudogene (Ochman & Davalos, 2006)noyesN460.139CYT0-0.200913-000iCytoplasmic8.9633.96
YAHF_ECOLIP77187yahFb0320 JW0312 Uncharacterized protein yahFPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN280.139CYT0-0.200913-000iCytoplasmic8.96
RNFB_ECOLIP77223rnfBydgMb1628 JW1620 Electron transport complex protein rnfBPeripheral inner membrane protein facing the cytoplasm (experimental).Electron transport. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCell inner membrane | Peripheral membrane proteinPeriplasmicyesyesN310.278SpI304.42273-10Yn3-14c19/20oCytoplasmicMembrane1022.68
LSRA_ECOLIP77257lsrAego ydeXb1513 JW1506 Uncharacterized protein yaiPPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicnoyesN420.176CYT0-0.200913-000iCytoplasmicMembrane1017.23
DDPD_ECOLIP77268ddpDyddPb1484 JW1479 Probable D,D-dipeptide transport ATP-binding protein ddpDPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN250.176CYT0-0.200913-000iCytoplasmicMembrane7.8823.21
YBBL_ECOLIP77279ybbLb0490 JW0479 Uncharacterized ABC transporter ATP-binding protein ybbLPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN330.126CYT0-0.200913-000iCytoplasmicMembrane9.8242.55
YDJJ_ECOLIP77280ydjJb1774 JW1763 Uncharacterized zinc-type alcohol dehydrogenase-like protein ydjJ (EC 1.-.-.-)Peripheral Inner membrane protein facing the cytoplasm (potential)No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Peripheral inner membrane protein facing the cytoplasmF1IM (predicted-detected)CytoplasmicnoyesN460.11CYT0-0.200913-000iCytoplasmic9.9777.2
HYFG_ECOLIP77329hyfGb2487 JW2472 Hydrogenase-4 component G (EC 1.-.-.-)Phobius predicts a slight hydrophobic area within the body proteinPeripheral inner membrane protein facing the cytoplasmF1IM (predicted)CytoplasmicyesyesN270.133CYT0-0.200913-000iCytoplasmic9.97
ARNA_ECOLIP77398arnApmrI yfbGb2255 JW2249 Bifunctional polymyxin resistance protein ArnA (Polymyxin resistance protein PmrI) [Includes: UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13) (ArnAFT) (UDP-L-Ara4N formyltransferase); UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acidPeripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN590.178CYT0-0.200913-000iCytoplasmic9.973.48
FADJ_ECOLIP77399fadJyfcXb2341 JW2338 Fatty acid oxidation complex subunit alpha [Includes: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC 4.2.1.17) (EC 5.1.2.3); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN700.113CYT0-0.200913-000iCytoplasmic9.9715.86
PTFX1_ECOLIP77439fryAypdDb2383 JW2380 Multiphosphoryl transfer protein 1 (MTP 1) [Includes: Phosphoenolpyruvate-protein phosphotransferase (EC 2.7.3.9) (Phosphotransferase system enzyme I); Phosphocarrier protein HPr (Protein H); Fructose-like phosphotransferase enzyme IIA component 2 (EC 2.7Peripheral inner membrane protein facing the cytoplasm Multiphosphoryl transfer protein 1 Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicnoyesN170.156CYT0-0.200913-00Yn2-10c16/17oCytoplasmic9.9738.59
SUFS_ECOLIP77444sufScsdB ynhBb1680 JW1670 Cysteine desulfurase (EC 2.8.1.7) (Selenocysteine beta-lyase) (SCL) (Selenocysteine lyase) (EC 4.4.1.16) (Selenocysteine reductase)Peripheral inner membrane protein facing the cytoplasm.SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS probably through direct interaction.[12941942]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN660.18CYT0-0.200913-000iCytoplasmic1011.17
YCJV_ECOLIP77481ycjVymjBb4524 JW1311/JW5203 b1318Putative uncharacterized ABC transporter ATP-binding protein ycjVPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicpseudogene (Uniprot)yesnoN370.103CYT0-0.200913-000iCytoplasmicMembrane9.9910.92
DXS_ECOLIP77488dxsyajPb0420 JW0410 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7) (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS)Peripheral inner membrane protein facing the cytoplasm (experimental). Cytoplasm YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300][23230279] [17272300]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN520.114CYT0-0.200913-000iCytoplasmic9.9721.77
PRPE_ECOLIP77495prpEyahUb0335 JW0326 Propionate--CoA ligase (EC 6.2.1.17) (Propionyl-CoA synthetase)Peripheral inner membrane protein facing the cytoplasm (potential).Homolog to FadD13 protein of Mycobacterium that is peripheral[22560731].[22560731]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicnoyesN440.132CYT0-0.200913-000iCytoplasmic9.9713.92
SUFC_ECOLIP77499sufCynhDb1682 JW1672 Probable ATP-dependent transporter sufCPeripheral inner membrane protein facing the cytoplasm. CytoplasmSufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction. Homolog to import ATP-binding protein MalK. [12554644][12941942] [12554644]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmCytoplasmicyesyesN320.213CYT0-0.200913-000iCytoplasmic1069.09
YPHE_ECOLIP77509yphEb2547 JW2531 Uncharacterized ABC transporter ATP-binding protein yphEPeripheral inner membrane protein facing the cytoplasm.Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN260.136CYT0-0.200913-000iCytoplasmicMembrane7.885
SUFB_ECOLIP77522sufBynhEb1683 JW5273 FeS cluster assembly protein sufBPeripheral inner membrane protein.Completely soluble no evidence for hyrophobic patches. IM binding determined by immunolocalization.[11943156]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN310.117CYT0-0.200913-000iUnknown243.02
RNFC_ECOLIP77611rnfCydgNb1629 JW1621 Electron transport complex protein rnfCPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN540.101CYT0-0.200913-000iCytoplasmic8.96
DDPF_ECOLIP77622ddpFyddOb1483 JW1478 Probable D,D-dipeptide transport ATP-binding protein ddpFPeripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN320.115CYT0-0.200913-000iCytoplasmicMembrane9.9918.26
ARCM_ECOLIP77624yahIb0323 JW0315 Carbamate kinase-like protein yahIPeripheral inner membrane protein facing the cytoplasm (potential).Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN200.12CYT0-0.200913-000iCytoplasmic9.9782.53
SUFD_ECOLIP77689sufDynhCb1681 JW1671 FeS cluster assembly protein sufDPeripheral inner membrane protein.Forms complex with SufC and SufB.[10322040]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN670.127CYT0-0.200913-000iCytoplasmic8.9636.65
OPPF_ECOLIP77737oppFb1247 JW1239 Oligopeptide transport ATP-binding protein oppFPeripheral inner membrane protein facing the cytoplasm (experimental). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279][23230279]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN700.124CYT0-0.200913-000iCytoplasmicMembrane1017.92
YDIJ_ECOLIP77748ydiJb1687 JW1677 Uncharacterized protein ydiJPeripheral inner membrane protein facing the cytoplasm (experimental). Inner membrane (experimental).[23230279] [15919657]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM FractionCytoplasmicyesyesN290.119CYT0-0.200913-000iCytoplasmic9.972.67
YDCT_ECOLIP77795ydcTb1441 JW1436 Uncharacterized ABC transporter ATP-binding protein ydcTPeripheral inner membrane protein facing the cytoplasmHomolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN200.146CYT0-0.200913-000iCytoplasmicMembrane1026.17
YGHU_ECOLIQ46845yghUb2989 JW5492 Uncharacterized GST-like protein yghUPeripheral inner membrane protein facing the cytoplasm. Peripheral inner membrane protein facing the cytoplasm (experimental).Phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. localization peripheral inner membrane protein possibly also integral inner membrane/phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] [23230279]Peripheral inner membrane protein facing the cytoplasmF1N/AMembrane anchoredyesyesN240.332CYT0-0.200913-100iCytoplasmic9.2649.34
YQHD_ECOLIQ46856yqhDb3011 JW2978 Alcohol dehydrogenase yqhD (EC 1.1.1.-)Peripheral inner membrane protein facing the cytoplasm (experimental).[23230279]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN210.138CYT0-0.200913-000iCytoplasmic9.9791.94
CSDA_ECOLIQ46925csdAygdJb2810 JW2781 Cysteine sulfinate desulfinase (CSD) (EC 4.4.1.-)Ribosomal Peripheral inner membrane protein facing the cytoplasm.Assembly cofactor of ribosome; associate with the 50S subunit. Under cold-shock conditions CsdA participates as an alternative DEAD-box RNA helicase together with RNase E in a ‘cold shock’ RNA degradosome (Prud’homme-Genereux et al., 2004). It is therefore possible that CsdA may associate with the inner membrane via RNAseE.[21529161] [15554978]Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicyesyesN590.194CYT0-0.200913-000iCytoplasmic9.979.8
FDRA_ECOLIQ47208fdrAylbDb0518 JW0506 Protein fdrAPeripheral inner membrane protein facing the cytoplasm (potential).[7500942]Peripheral inner membrane protein facing the cytoplasmF1N/ACell membrane | Multi-pass membrane proteinIntegral Membrane ProteinyesyesN310.11CYT0-0.200913-000iUnknown256.6
TAUB_ECOLIQ47538tauBssiB yaiQb0366 JW0358 Taurine import ATP-binding protein TauB (EC 3.6.3.36)Peripheral inner membrane protein facing the cytoplasmF1N/ACell inner membrane | Peripheral membrane proteinCytoplasmicyesyesN570.124CYT0-0.200913-000iCytoplasmicMembrane1010.43
YTFR_ECOLIQ6BEX0ytfRytfSb4485 JW5752 Uncharacterized ABC transporter ATP-binding protein ytfRPeripheral inner membrane protein facing the cytoplasm.Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK. Peripheral inner membrane protein facing the cytoplasmF1N/ACytoplasmicpseudogene (Ochman & Davalos, 2006)noyesN250.128CYT0-0.200913-000iCytoplasmicMembrane1024.65
ADPP_ECOLIQ93K97nudFaspP yqiE yzzGb3034 JW3002 ADP-ribose pyrophosphatase (EC 3.6.1.13) (ADP-ribose diphosphatase) (ADP-ribose phosphohydrolase) (ASPPase) (Adenosine diphosphoribose pyrophosphatase) (ADPR-PPase)Peripheral inner membrane protein facing the cytoplasm (potential).Soluble protein, detected in inner membrane fraction[19766000].[19766000]Peripheral inner membrane protein facing the cytoplasmF1Soluble IM Fraction +CytoplasmicyesyesN380.101CYT0-0.200913-000iCytoplasmic8.9657.62