entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
SODM_ECOLIP00448
sodA
b3908 JW3879 Superoxide dismutase [Mn] (EC 1.15.1.1) (MnSOD)PeriplasmicThe superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are E.coli proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm.PMID: 21854464PeriplasmicGN/ACytoplasmicSEC, non classicalyesyesN510.145CYT0-0.200913-00="0"iUnknown5.41
BTUE_ECOLIP06610
btuE
b1710 JW1700 Vitamin B12 transport periplasmic protein BtuE (EC 1.-.-.-) Disulfide bond-containing protein PMID: 25664544PMID: 25664544PeriplasmicGN/APeriplasmCytoplasmicSEC, non classicalyesyesN370.111CYT0-0.200913-00="0"iPeriplasmic1099.42
YGFZ_ECOLIP0ADE8
ygfZ

yzzW

yzzWb2898 JW2866 tRNA-modifying protein ygfZPeriplasmic CytoplasmicSubcellular localization analysis of cytotoxic necrotizing factor 1 (CNF1) demonstrated that YgfZ, a periplasmic protein, contributes to secretion of CNF1 (a normally cytoplasmic protein) into outer-membrane vesicles (OMVs) in pathogenic E.coli strain RS218 O18:K1:H7 PMID: 22174383. PMID: 9298646PMID: 22174383 PMID: 9298646Cytoplasmic, PeriplasmicA, GN/ACytoplasmCytoplasmic (Experimental)SEC, non classicalyesyesN310.252CYT0-0.200913-00="0"iUnknown282.19
FTSL_ECOLIP0AEN4
ftsL

mraR yabD

mraR yabDb0083 JW0081 Cell division protein ftsLLipoprotein (experimental). Integral Inner Membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membrane Cell inner membraneLocalizes to potential division sites in filamentous cells. Proven pignal peptidase II substrate. PMID: 1332942PMID: 15174130 PMID: 21890895 PMID: 1332942 PMID: 10027987 PMID: 11703663 PMID: 11948172 PMID: 15165235Inner Membrane LipoproteinEIM (predicted)Cell inner membrane | Single-pass type II membrane proteinMembrane anchored (Experimental)SEC, non classicalyesyesN240.167CYT0-0.200913-11="0"o35-57iCytoplasmic8.9625.32
SODF_ECOLIP0AGD3
sodB
b1656 JW1648 Superoxide dismutase [Fe] (EC 1.15.1.1)PeriplasmThe superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are E.coli proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm. PeriplasmicGN/ACytoplasmicSEC, non classicalyesyesN170.122CYT0-0.200913-00="0"iPeriplasmic9.4477.73
PNCB_ECOLIP18133
pncB
b0931 JW0914 Nicotinate phosphoribosyltransferase (NAPRTase) (EC 2.4.2.11)Periplasm.PncB is localized in the periplasm although it does not possess a signal peptide.PMID: 346557PeriplasmicGN/ACytoplasmCytoplasmicSEC, non classicalnoyesN610.11CYT0-0.200913-00="0"iCytoplasmic9.9732.26
SPEA_ECOLIP21170
speA
b2938 JW2905 Biosynthetic arginine decarboxylase (ADC) (EC 4.1.1.19)Peripheral inner membrane protein facing the cytoplasm (potential). Periplasmic Peripheral inner membrane protein facing the cytoplasm (potential). PeriplasmTrigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 2198270 Selective disruption of the cell envelope and an assessment of Spea activity or immunoprecipitable SpeA in various fractions demonstrated its location between the cytoplasmic membrane and peptidoglycan layer. PMID: 3894328PMID: 19737520 PMID: 15919657 PMID: 2198270 PMID: 19766000 PMID: 3894328PeriplasmicGSoluble IM FractionPeriplasmCytoplasmicTFSEC, non classicalyesyesN270.129CYT0-0.200913-00="0"iPeriplasmic1048
EAEH_ECOLIP36943
eaeH
b0297 JW0291 Putative attaching and effacing protein homologPeriplasmicUniprot gives portential signal peptide (1-25) although it is not predicted by any tool. Intimin homologue but missing the C-terminus. Intimin is in the OM. Could be periplasmic. The signal peptide could be long autotransporter-like signal peptide. Intimin does not have a classical signal peptide either. PeriplasmicGN/AMembrane anchoredSEC, non classicalpseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN450.383CYT0-0.200913-11="0"i21-44oOuterMembrane9.925.4
YCGI_ECOLIP76000
ycgI

ymgH

ymgHb4521 JW1162/JW5179 b1173Putative uncharacterized protein ycgIContains a predicted autotransporter domain. Potential outer membrane b-barrel protein though it does not have prediction for a signal peptide. Contains autotransporter beta-domain (IPR005546)Outer Membrane b-barrel proteinHN/ACytoplasmCytoplasmicSEC, non classicalAutotransporter(T5S)pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)nonoN250.12CYT0-0.200913-00="0"iUnknown712.67
YCGV_ECOLIP76017
ycgV
b1202 JW1193 Uncharacterized protein ycgVOuter membraneAg43 homologue; known autotransporter protein. Signal peptide 1-56 (by clustalW). Contains autotransporter beta-domain (IPR005546). Potential Srp-dependent autotransporter. Homologous to hemoglobin-binding protease hbp. Hbp is the only known autotransporter that is secreted through the integral membrane in a Srp-dependent manner.PMID: 15590781 PMID: 12466262Outer Membrane b-barrel proteinHIM (predicted)CytoplasmicSRPSEC, non classicalAutotransporter(T5S)noyesN470.309CYT0-0.200913-01="0"i27-47oExtracellular9.4617.39
MDTA_ECOLIP76397
mdtA

yegM

yegMb2074 JW5338 Multidrug resistance protein mdtA (Multidrug transporter mdtA)Inner membrane.Part of MdtABC-TolC multidrug efflux transport system = [(TolC)3][MdtC][MdtB][MdtA].PMID: 12107133 PMID: 12107134 PMID: 21890895Peripheral inner membrane protein facing the periplasmF2N/ACell inner membrane | Peripheral membrane proteinMembrane anchoredSEC, non classicalyesyesN220.151TMH07.31567-11="0"i9-26oCytoplasmicMembrane9.8237.79
YDEU_ECOLIP77286
ydeU
b1509 JW1502 Uncharacterized protein YdeUAg43 homologue; predicted autotransporter.PMID: 15590781Outer Membrane b-barrel proteinHN/ACytoplasmicSEC, non classicalAutotransporter(T5S)noyesN330.107CYT0-0.200913-00="0"iOuterMembrane9.833.64