entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
FHUD_ECOLIP07822
fhuD
b0152 JW0148 Iron(3+)-hydroxamate-binding protein fhuD (Ferric hydroxamate uptake protein D) (Ferrichrome-binding periplasmic protein) (Iron(III)-hydroxamate-binding protein fhuD)Periplasmic TAT secretion system. PMID: 8522527 Iron ion binding protein PMID: 17218314 PMID: 8522527 PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECyesyesY310.749SpI308.81274-00Yn12-23c30/31oPeriplasmic10
YNFG_ECOLIP0AAJ1
ynfG
b1589 JW1581 Probable anaerobic dimethyl sulfoxide reductase chain ynfG (DMSO reductase iron-sulfur subunit ynfG)Peripheral inner membrane protein facing the periplasm.The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. ynfFGH are expressed anaerobically. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. YnfH can function with DmsAB, replacing DmsC as the anchor subunit. The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.PMID: 14522592PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/APeriplasmicTAT piggybackyesyesN440.106CYT0-0.200913-00="0"iUnknown5.4138.9
HYBA_ECOLIP0AAJ8
hybA
b2996 JW2964 Hydrogenase-2 operon protein hybAOuter membrane protein (experimental). Inner membrane. PeriplasmicTAT secretion system. Part of membrane-associated hydrogenase 4 complex. PMID: 9738917PMID: 15911532 PMID: 22313414 PMID: 21890895 PMID: 9738917Peripheral inner membrane protein facing the periplasmF2IM (predicted)PeriplasmPeriplasmic (Experimental)TAT,SECyesyesY270.442SpI2619.7463-01Yn9-20c26/27o293-311iCytoplasmicMembrane9.9739.36
NRFC_ECOLIP0AAK7
nrfC

yjcJ

yjcJb4072 JW4033 Protein nrfCPeriplasmicTAT secretion system. PMID: 15280383PMID: 17218314 PMID: 15280383PeriplasmicGN/APeriplasmic (Experimental)TATyesyesN280.447SpI277.45987-00Yn8-19c27/28oCytoplasmic1023.27
NAPG_ECOLIP0AAL3
napG

yojA yojB

yojA yojBb2205 JW2193 Ferredoxin-type protein napGPeriplasmicTAT secretion system. PMID: 11967083PMID: 17218314 PMID: 11967083PeriplasmicGN/APeriplasmic (Experimental)TATyesyesN420.316SpI410.19393-00Yn21-32c41/42oCytoplasmic8.9620.35
YCBK_ECOLIP0AB06
ycbK
b0926 JW0909 Uncharacterized protein ycbKTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTATyesyesN310.559SpI308.29508-10Yn11-22c30/31oUnknown2.511.19
YFHG_ECOLIP0AD44
yfhG
b2555 JW2539 Uncharacterized protein yfhGTAT secretion system.PMID: 17218314Outer Membrane LipoproteinIN/AOuter membrane LipoproteinTAT,SECLOLyesyesY230.159SpII256.96106Pos+2=V00Yn13-24c29/30oUnknown255.19
MBHM_ECOLIP0ACE0
hybC
b2994 JW2962 Hydrogenase-2 large chain (HYD2) (EC 1.12.99.6) (Membrane-bound hydrogenase 2 large subunit) (NiFe hydrogenase)Peripheral inner membrane protein facing the periplasm (experimental). Peripheral inner membrane protein facing the periplasm (experimental). Periplasmic The protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system piggyback. PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544PMID: 10224080 PMID: 3516690 PMID: 8772179 PMID: 9738917 PMID: 25664544Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane | Peripheral membrane proteinPeriplasmic (Experimental)TAT piggybackyesyesN340.139CYT0-0.200913-00="0"iCytoplasmicMembrane1028.87
DMSA_ECOLIP18775
dmsA
b0894 JW5118 Anaerobic dimethyl sulfoxide reductase chain A (DMSO reductase) (EC 1.8.99.-)Periplasmic Peripheral inner membrane protein facing the cytoplasm or the periplasm. Cell membrane, Peripheral membrane protein, Cytoplasmic side TAT signal peptide, dmsABC complex. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD. PMID: 24093457 Fluorescence analysis of these YFP chimeras revealed a wide range of interactions such as the one between the Tat substrate dimethyl sulfoxide reductase (DmsA) and its dedicated proofreading chaperone DmsD. PMID: 20169075 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395. TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332. PMID: 3280546 PMID: 3062312 Interacts with DmsD, a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 17218314 PMID: 3062312 PMID: 11389150 PMID: 24093457 PMID: 20169075 PMID: 9546395 PMID: 2170332 PMID: 3280546 PMID: 20153451Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane | Peripheral membrane protein | Cytoplasmic sidePeriplasmic (Experimental)DmsDTAT,SECyesyesN460.537SpI455.24058-00="0"iCytoplasmicMembrane1022.52
DMSB_ECOLIP18776
dmsB
b0895 JW0878 Anaerobic dimethyl sulfoxide reductase chain B (DMSO reductase iron-sulfur subunit)Peripheral inner membrane protein facing the cytoplasm or the periplasm. TAT piggyback; DmsB is co-exported bound to Tat substrate DmsA. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332.PMID: 3062312 PMID: 11389150 PMID: 9546395 PMID: 2170332Peripheral inner membrane protein facing the periplasmF2N/ACytoplasmicTAT piggybackyesyesN440.107CYT0-0.200913-00="0"iUnknown5.4127.7
FDNG_ECOLIP24183
fdnG
b1474 JW1470 Formate dehydrogenase, nitrate-inducible, major subunit (EC 1.2.1.2) (Anaerobic formate dehydrogenase major subunit) (Formate dehydrogenase-N subunit alpha) (FDH-N subunit alpha)Peripheral inner membrane protein facing the cytoplasm (experimental). Membrane associated Membrane associated Membrane associatedTAT secretion system; Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site. FdnG is the catalytic subunit of formate dehydrogenase-N (FdnGHI). FdnG was found in the periplasmic fraction of a mutant lacking the membrane anchor subunit FdnI, confirming that FdnG is located at the periplasmic face of the cytoplasmic membrane. PMID: 11929547 PMID: 11884747 PMID: 12923080 PMID: 11884747 PMID: 16858726PMID: 17218314 PMID: 15342602 PMID: 11929547 PMID: 12923080 PMID: 11884747 PMID: 16858726Peripheral inner membrane protein facing the periplasmF2N/APeriplasmMembrane associated (Experimental)TATnoyesN340.342SpI336.74593-00Yn11-24c33/34oPeriplasmic10
FTSP_ECOLIP26648
ftsP

sufI

sufIb3017 JW2985 Protein sufIPeriplasm Periplasm Periplasmic TAT secretion system. PMID: 9298646 Copper ion binding protein PMID: 17218314 PMID: 10593889 PMID: 19135451 PMID: 9298646 PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECnoyesY280.494SpI2710.6124-00Yn8-19c27/28oPeriplasmic108.07
EFEB_ECOLIP31545
efeB

ycdB

ycdBb1019 JW1004 Peroxidase ycdB (EC 1.11.1.-)PeriplasmTAT secretion system.PMID: 17218314 PMID: 16551627PeriplasmicGN/APeriplasmPeriplasmicTAT,SECyesyesY360.748SpI355.64598-00Yn20-30c35/36oPeriplasmic1033.84
FDOG_ECOLIP32176
fdoG
b3894 JW3865 Formate dehydrogenase-O major subunit (EC 1.2.1.2) (Aerobic formate dehydrogenase major subunit) (FDH-Z subunit alpha) (Formate dehydrogenase-O subunit alpha)PeriplasmicTAT secretion system. PMID: 12923080PMID: 17218314 PMID: 12923080PeriplasmicGSoluble IM FractionPeriplasmPeriplasmic (Experimental)TATnoyesN340.487SpI3314.6779-00Yn11-24c33/34oPeriplasmic10
TORA_ECOLIP33225
torA
b0997 JW0982 Trimethylamine-N-oxide reductase 1 (TMAO reductase 1) (Trimethylamine oxidase 1) (EC 1.7.2.3)Periplasm PeriplasmicTAT secretion system. Trimethylamine N-oxide (TMAO) respiration system is encoded by the torCAD operon and comprises a periplasmic TMAO reductase (TorA) and a c-type cytochrome (TorC), which shuttles electrons to TorA. Interaction studies showed that, when immature, TorC can no longer bind TorA efficiently but can bind the periplasmic detector region of sensor TorS. Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase TorD, a cytoplasmic chaperone that interacts with the unfolded trimethylamine N-oxide reductase enzyme (TorA). The absence of TorD protein leads to a two times decrease of the final amount of TorA enzyme. PMID: 15073303PMID: 17218314 PMID: 2512991 PMID: 11056172 PMID: 9632735 PMID: 15073303PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TATyesyesN400.294SpI391.32729-00Yn14-24c42/43oPeriplasmic108.51
NAPA_ECOLIP33937
napA

yojC yojD yojE

yojC yojD yojEb2206 JW2194 Periplasmic nitrate reductase (EC 1.7.99.4)Periplasm Periplasm TAT secretion system. Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 17218314 PMID: 10234835 PMID: 11844760 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TATyesyesY320.617SpI3120.6365-00Yn13-23c31/32oPeriplasmic10
AMIA_ECOLIP36548
amiA

yfeE

yfeEb2435 JW2428 Probable N-acetylmuramoyl-L-alanine amidase AmiA (EC 3.5.1.28)PeriplasmThe Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathwayPMID: 17218314PMID: 12787347. PMID: 12787347PMID: 17218314 PMID: 12787347PeriplasmicGN/ASecretedPeriplasmic (Experimental)TAT,SECyesyesY350.684SpI3415.9519-00Yn16-26c34/35oUnknown2.536.61
CUEO_ECOLIP36649
cueO

yacK

yacKb0123 JW0119 Blue copper oxidase cueO (Copper efflux oxidase)PeriplasmicTAT secretion system. PMID: 9298646PMID: 17218314 PMID: 9298646PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECyesyesY290.821SpI2816.1248-00Yn11-22c28/29oPeriplasmic106.35
OPGD_ECOLIP40120
mdoD

opgD ydcG yzzZ

opgD ydcG yzzZb1424 JW1420 Glucans biosynthesis protein DPeriplasmic TAT secretion system. PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 17218314 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECnoyesY330.683SpI3218.7932-00Yn14-25c32/33oPeriplasmic1061.48
TORZ_ECOLIP46923
torZ

bisZ

bisZb1872 JW1861 Trimethylamine-N-oxide reductase 2 (TMAO reductase 2) (Trimethylamine oxidase 2) (EC 1.7.2.3)PeriplasmicTAT secretion system. PMID: 11004177PMID: 17218314 PMID: 11004177PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECyesyesN320.688SpI3117.7989-00Yn12-23c31/32oPeriplasmic1033.07
AMIC_ECOLIP63883
amiC

ygdN

ygdNb2817 JW5449 N-acetylmuramoyl-L-alanine amidase AmiC (EC 3.5.1.28)PeriplasmTAT secretion system. PMID: 12787347PMID: 17218314 PMID: 12787347PeriplasmicGN/ASecretedPeriplasmic (Experimental)TAT,SECyesyesN320.61SpI319.65072-00Yn13-23c31/32oUnknown233.02
MBHT_ECOLIP69741
hybO

yghV

yghVb2997 JW2965 Hydrogenase-2 small chain (HYD2) (EC 1.12.99.6) (Membrane-bound hydrogenase 2 small subunit) (NiFe hydrogenase)Membrane anchored Peripheral inner membrane protein facing the cytoplasm.Copurifies with trigger factor in pull down assays. Exported by the Tat system. Experimentally proven signal peptide cleavage site. Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. PMID: 22769583 PMID: 9738917PMID: 17218314 PMID: 19737520 PMID: 9738917 PMID: 22769583Peripheral inner membrane protein facing the periplasmF2IM (predicted)Cell membrane | Peripheral membrane protein | Periplasmic sidePeriplasmMembrane anchored (Experimental)TFTAT,SECyesyesN380.248SpI377.00921-11Yn21-32c37/38o332-355iCytoplasmicMembrane10
WCAM_ECOLIP71244
wcaM

yefK

yefKb2043 JW2028 Colanic acid biosynthesis protein wcaMTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTATnoyesY290.421SpI2815.7954-00Yn10-21c28/29oUnknown2.5
YEDY_ECOLIP76342
yedY
b1971 JW1954 Sulfoxide reductase catalytic subunit yedY (EC 1.8.-.-)Peripheral inner membrane protein facing the cytoplasm (experimental). Membrane anchoredTAT secretion system. PMID: 15355966PMID: 17218314 PMID: 16042411 PMID: 15355966Peripheral inner membrane protein facing the periplasmF2N/APeriplasmMembrane anchored (Experimental)TAT,SECnoyesN450.526SpI447.04958-00Yn28-38c44/45oUnknown2.57.22
YAGT_ECOLIP77165
yagT
b0286 JW0280 Putative xanthine dehydrogenase yagT iron-sulfur-binding subunitPeriplasmTAT secretion system. YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm. Tat substrates are matured in the cytoplasm prior to their translocation. PMID: 19368556PMID: 17218314 PMID: 19368556PeriplasmicGN/ACytoplasmicTATnoyesN490.297CYT0-0.200913-00="0"iUnknown4.9989.32
YAGS_ECOLIP77324
yagS
b0285 JW0279 Putative xanthine dehydrogenase yagS FAD-binding subunit (EC 1.17.1.4)PeriplasmTAT piggyback The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm (data not shown). Tat substrates are matured in the cytoplasm prior to their translocation. The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm (data not shown). PMID: 19368556PMID: 19368556 PMID: 17218314 PMID: 19368556PeriplasmicGN/AMembrane anchoredTAT piggybacknoyesN160.129CYT0-0.200913-00="0"iCytoplasmic9.2615.35
YNFE_ECOLIP77374
ynfE
b1587 JW1579 Putative dimethyl sulfoxide reductase chain ynfE (DMSO reductase) (EC 1.8.99.-)TAT secretion system. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. ynfFGH are expressed anaerobically. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. YnfH can function with DmsAB, replacing DmsC as the anchor subunit. The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.PMID: 14522592PMID: 17218314 PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/ACell membrane | Peripheral membrane protein | Cytoplasmic sidePeriplasmicTATyesyesN280.177SpI424.96127-00Yn19-37c42/43oCytoplasmicMembrane9.9821.02
YDHX_ECOLIP77375
ydhX
b1671 JW5271 Uncharacterized ferredoxin-like protein ydhXTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTAT,SECyesyesN280.293SpI276.80968-10Yn8-19c27/28oUnknown4.99
YAGR_ECOLIP77489
yagR
b0284 JW0278 Putative xanthine dehydrogenase yagR molybdenum-binding subunit (EC 1.17.1.4)PeriplasmTAT piggyback The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm. Tat substrates are matured in the cytoplasm prior to their translocation.PMID: 19368556PeriplasmicGN/APeriplasmicTAT piggybacknoyesN600.121CYT0-0.200913-00="0"iCytoplasmicMembrane9.8227.97
YAHJ_ECOLIP77554
yahJ
b0324 JW0316 Uncharacterized protein yahJTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTAT,SECnoyesY340.674SpI3311.0447-00Yn18-28c33/34oUnknown4.9912.17
YNFF_ECOLIP77783
ynfF
b1588 JW5260 Probable dimethyl sulfoxide reductase chain ynfF (DMSO reductase) (EC 1.8.99.-)Verified Tat substrate: YnfF has a Tat-only (Class II) predicted 45 aa signal peptide PMID: 17218314. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. ynfFGH are expressed anaerobically. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. YnfH can function with DmsAB, replacing DmsC as the anchor subunit. The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.PMID: 14522592PMID: 17218314 PMID: 19415239 PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/ACell membrane | Peripheral membrane protein | Cytoplasmic sidePeriplasmicTAT,SECyesyesN460.406SpI4511.1244-00Yn24-37c45/46oCytoplasmicMembrane9.999.59