entryaccgenealt_geneolnprotein_namecm_locacm_notescm_refssuboci_fullsublocisubloci_iimnterm_ctermbernselsubloci_uniprotsubloci_echoLorganelletarget_chaperoning11st_sstarget_chaperoning22nd_sspseudocommon_corebasic_proteomesignalp_sp_existsignalp_possignalp_cmaxlipop_predlipop_cleavagelipop_scorelipop_ruletmhmmphobius_tmsphobius_sp_existphobius_predictionpsortb_locapsortb_scoresolubility
FHUD_ECOLIP07822
fhuD
b0152 JW0148 periplasmic protein (TAT-SEC through IM), Iron(3+)-hydroxamate-binding FhuDPeriplasmicTAT secretion system. PMID: 8522527PMID: 17218314 PMID: 8522527 PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECyesyesY310.749SpI308.81274-00Yn12-23c30/31oPeriplasmic10
YNFG_ECOLIP0AAJ1
ynfG
b1589 JW1581 periplasmic peripheral IM protein (TAT via piggyback through IM), Probable anaerobic dimethyl sulfoxide reductase chain YnfG Peripheral inner membrane protein facing the periplasmThe physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown. DmsB paralog. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. ynfFGH are expressed anaerobically. YnfG can function with DmsAC, replacing DmsB a as the electron transfer subunit. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/APeriplasmicTAT (piggyback)yesyesN440.106CYT0-0.200913-00NiUnknown538
HYBA_ECOLIP0AAJ8
hybA
b2996 JW2964 periplasmic peripheral IM protein (TAT-SEC through IM), Hydrogenase-2 operon HybAPeripheral inner membrane protein facing the periplasmTAT secretion system. Part of membrane-associated hydrogenase 4 complex. PMID: 9738917PMID: 15911532 PMID: 22313414 PMID: 21890895 PMID: 9738917Peripheral inner membrane protein facing the periplasmF2IM (predicted)PeriplasmPeriplasmic (Experimental)TAT,SECyesyesY270.442SpI2619.7463-01Yn9-20c26/27o293-311iCytoplasmicMembrane939
NRFC_ECOLIP0AAK7
nrfC

yjcJ

yjcJb4072 JW4033 periplasmic protein (TAT through IM), NrfCPeriplasmicTAT secretion system. PMID: 15280383PMID: 17218314 PMID: 15280383PeriplasmicGN/APeriplasmic (Experimental)TATyesyesN280.447SpI277.45987-00Yn8-19c27/28oCytoplasmic1023
NAPG_ECOLIP0AAL3
napG

yojA yojB

yojA yojBb2205 JW2193 periplasmic protein (TAT through IM), Ferredoxin-type NapGPeriplasmicTAT secretion system. PMID: 11967083PMID: 17218314 PMID: 11967083PeriplasmicGN/APeriplasm Periplasmic (Experimental)TATyesyesN420.316SpI410.19393-00Yn21-32c41/42oCytoplasmic820
YCBK_ECOLIP0AB06
ycbK
b0926 JW0909 periplasmic protein (TAT through IM), YcbKPeriplasmicTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTATyesyesN310.559SpI308.29508-10Yn11-22c30/31oUnknown211
MBHM_ECOLIP0ACE0
hybC
b2994 JW2962 periplasmic peripheral IM protein (TAT via piggyback through IM), Hydrogenase-2 large chain HybCPeripheral inner membrane protein facing the periplasmThe protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system (piggyback). PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544PMID: 10224080 PMID: 3516690 PMID: 8772179 PMID: 9738917 PMID: 25664544Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane; Peripheral membrane proteinPeriplasmic (Experimental)TAT (piggyback)yesyesN340.139CYT0-0.200913-00NiCytoplasmicMembrane1028
YFHG_ECOLIP0AD44
yfhG
b2555 JW2539 OM lipoprotein (TAT-SEC through IM, inserted in OM by LOL), YfhGOuter Membrane LipoproteinTAT secretion system.PMID: 17218314Outer Membrane LipoproteinIN/AOuter membrane LipoproteinTAT,SECLOLyesyesY230.159SpII256.96106Pos+2=V00Yn13-24c29/30oUnknown255
DMSA_ECOLIP18775
dmsA
b0894 JW5118 periplasmic peripheral IM protein (TAT-SEC through IM), Dimethyl sulfoxide reductase DmsAPeripheral inner membrane protein facing the periplasmTAT signal peptide, dmsABC complex. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD. PMID: 24093457 Fluorescence analysis of these YFP chimeras revealed a wide range of interactions such as the one between the Tat substrate dimethyl sulfoxide reductase (DmsA) and its dedicated proofreading chaperone DmsD. PMID: 20169075 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 PMID: 3280546 PMID: 3062312 Interacts with DmsD, a redox enzyme maturation protein (REMP). PMID: 20153451PMID: 17218314 PMID: 3062312 PMID: 11389150 PMID: 24093457 PMID: 20169075 PMID: 9546395 PMID: 2170332 PMID: 3280546 PMID: 20153451Peripheral inner membrane protein facing the periplasmF2Soluble IM FractionCell membrane Periplasmic (Experimental)DmsDTAT,SECyesyesN460.537SpI455.24058-00NiCytoplasmicMembrane1022
DMSB_ECOLIP18776
dmsB
b0895 JW0878 periplasmic peripheral IM protein (TAT via piggyback through IM), Anaerobic dimethyl sulfoxide reductase chain B DmsBPeripheral inner membrane protein facing the periplasmTAT (piggyback); DmsB is co-exported bound to Tat substrate DmsA. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332PMID: 3062312 PMID: 11389150 PMID: 9546395 PMID: 2170332Peripheral inner membrane protein facing the periplasmF2N/ACytoplasmicTAT (piggyback)yesyesN440.107CYT0-0.200913-00NiUnknown527
FDNG_ECOLIP24183
fdnG
b1474 JW1470 periplasmic peripheral IM protein (TAT through IM), Formate dehydrogenase, nitrate-inducible, major subunit FdnGPeripheral inner membrane protein facing the periplasmTAT secretion system; Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site. FdnG is the catalytic subunit of formate dehydrogenase-N (FdnGHI). FdnG was found in the periplasmic fraction of a mutant lacking the membrane anchor subunit FdnI, confirming that FdnG is located at the periplasmic face of the cytoplasmic membrane. PMID: 11929547 PMID: 11884747 PMID: 12923080 PMID: 11884747 PMID: 16858726PMID: 17218314 PMID: 15342602 PMID: 11929547 PMID: 12923080 PMID: 11884747 PMID: 16858726Peripheral inner membrane protein facing the periplasmF2N/APeriplasm Membrane associated (Experimental)TATnoyesN340.342SpI336.74593-00Yn11-24c33/34oPeriplasmic10
FTSP_ECOLIP26648
ftsP

sufI

sufIb3017 JW2985 periplasmic protein (TAT-SEC through IM), Cell division FtsPPeriplasmicTAT secretion system. PMID: 9298646PMID: 17218314 PMID: 10593889 PMID: 19135451 PMID: 9298646 PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECnoyesY280.494SpI2710.6124-00Yn8-19c27/28oPeriplasmic108
EFEB_ECOLIP31545
efeB

ycdB

ycdBb1019 JW1004 periplasmic protein (TAT-SEC through IM), Deferrochelatase/peroxidase EfeBPeriplasmicTAT secretion system.PMID: 17218314 PMID: 16551627PeriplasmicGN/APeriplasm PeriplasmicTAT,SECyesyesY360.748SpI355.64598-00Yn20-30c35/36oPeriplasmic1033
FDOG_ECOLIP32176
fdoG
b3894 JW3865 periplasmic protein (TAT through IM), Formate dehydrogenase-O major subunit FdoGPeriplasmicTAT secretion system. PMID: 12923080PMID: 17218314 PMID: 12923080PeriplasmicGSoluble IM FractionPeriplasm Periplasmic (Experimental)TATnoyesN340.487SpI3314.6779-00Yn11-24c33/34oPeriplasmic10
TORA_ECOLIP33225
torA
b0997 JW0982 periplasmic protein (TAT through IM), Trimethylamine-N-oxide reductase 1 TorAPeriplasmicTAT secretion system. Trimethylamine N-oxide (TMAO) respiration system is encoded by the torCAD operon and comprises a periplasmic TMAO reductase (TorA) and a c-type cytochrome (TorC), which shuttles electrons to TorA. Interaction studies showed that, when immature, TorC can no longer bind TorA efficiently but can bind the periplasmic detector region of sensor TorS. Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase TorD, a cytoplasmic chaperone that interacts with the unfolded trimethylamine N-oxide reductase enzyme (TorA). The absence of TorD protein leads to a two times decrease of the final amount of TorA enzyme. PMID: 15073303PMID: 17218314 PMID: 2512991 PMID: 11056172 PMID: 9632735 PMID: 15073303PeriplasmicGN/APeriplasm Periplasmic (Experimental)TATyesyesN400.294SpI391.32729-00Yn14-24c42/43oPeriplasmic108
NAPA_ECOLIP33937
napA

yojC yojD yojE

yojC yojD yojEb2206 JW2194 periplasmic protein (TAT through IM), nitrate reductase NapAPeriplasmicTAT secretion system. Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544PMID: 17218314 PMID: 10234835 PMID: 11844760 PMID: 25664544PeriplasmicGN/APeriplasm Periplasmic (Experimental)TATyesyesY320.617SpI3120.6365-00Yn13-23c31/32oPeriplasmic10
AMIA_ECOLIP36548
amiA

yfeE

yfeEb2435 JW2428 periplasmic protein (TAT-SEC through IM), N-acetylmuramoyl-L-alanine amidase AmiA PeriplasmicThe Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathwayPMID: 17218314PMID: 12787347. PMID: 12787347PMID: 17218314 PMID: 12787347PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECyesyesY350.684SpI3415.9519-00Yn16-26c34/35oUnknown236
CUEO_ECOLIP36649
cueO

yacK

yacKb0123 JW0119 periplasmic protein (TAT-SEC through IM), Blue copper oxidase CueOPeriplasmicTAT secretion system. PMID: 9298646PMID: 17218314 PMID: 9298646PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECyesyesY290.821SpI2816.1248-00Yn11-22c28/29oPeriplasmic106
OPGD_ECOLIP40120
mdoD

opgD ydcG yzzZ

opgD ydcG yzzZb1424 JW1420 periplasmic protein (TAT-SEC through IM), Glucans biosynthesis D MdoDPeriplasmicTAT secretion system. PMID: 9298646 Disulfide bond-containing protein PMID: 25664544PMID: 17218314 PMID: 9298646 PMID: 25664544PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECnoyesY330.683SpI3218.7932-00Yn14-25c32/33oPeriplasmic1061
TORZ_ECOLIP46923
torZ

bisZ

bisZb1872 JW1861 periplasmic protein (TAT-SEC through IM), Trimethylamine-N-oxide reductase 2 TorZPeriplasmicTAT secretion system. PMID: 11004177PMID: 17218314 PMID: 11004177PeriplasmicGN/APeriplasmPeriplasmic (Experimental)TAT,SECyesyesN320.688SpI3117.7989-00Yn12-23c31/32oPeriplasmic1033
AMIC_ECOLIP63883
amiC

ygdN

ygdNb2817 JW5449 periplasmic protein (TAT-SEC through IM), N-acetylmuramoyl-L-alanine amidase AmiC PeriplasmicTAT secretion system. PMID: 12787347PMID: 17218314 PMID: 12787347PeriplasmicGN/APeriplasm Periplasmic (Experimental)TAT,SECyesyesN320.61SpI319.65072-00Yn13-23c31/32oUnknown233
MBHT_ECOLIP69741
hybO

yghV

yghVb2997 JW2965 periplasmic peripheral IM protein (TAT-SEC through IM), Hydrogenase-2 small chain HybOPeripheral inner membrane protein facing the periplasmCopurifies with trigger factor in pull down assays. Exported by the Tat system. Experimentally proven signal peptide cleavage site. Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. PMID: 22769583 PMID: 9738917PMID: 17218314 PMID: 19737520 PMID: 9738917 PMID: 22769583Peripheral inner membrane protein facing the periplasmF2IM (predicted)Cell membrane; Peripheral membrane protein; Periplasmic side PeriplasmMembrane anchored (Experimental)TFTAT,SECyesyesN380.248SpI377.00921-11Yn21-32c37/38o332-355iCytoplasmicMembrane10
WCAM_ECOLIP71244
wcaM

yefK

yefKb2043 JW2028 periplasmic protein (TAT through IM), Colanic acid biosynthesis WcaMPeriplasmicTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTATnoyesY290.421SpI2815.7954-00Yn10-21c28/29oUnknown2
MSRP_ECOLIP76342
msrP

yedY

yedYb1971 JW1954 periplasmic peripheral IM protein (TAT-SEC through IM), Protein-methionine-sulfoxide reductase catalytic subunit MsrP Peripheral inner membrane protein facing the periplasmTAT secretion system. PMID: 15355966PMID: 17218314 PMID: 16042411 PMID: 15355966Peripheral inner membrane protein facing the periplasmF2N/APeriplasm Membrane anchored (Experimental)TAT,SECnoyesN450.526SpI447.04958-00Yn28-38c44/45oUnknown27
YAGT_ECOLIP77165
yagT
b0286 JW0280 periplasmic protein (TAT through IM), Aldehyde oxidoreductase iron-sulfur-binding subunit YagTPeriplasmicTAT secretion system. YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm. Tat substrates are matured in the cytoplasm prior to their translocation. PMID: 19368556PMID: 17218314 PMID: 19368556PeriplasmicGN/APeriplasm CytoplasmicTATnoyesN490.297CYT0-0.200913-00NiUnknown489
YAGS_ECOLIP77324
yagS
b0285 JW0279 periplasmic protein (TAT via piggyback through IM), Aldehyde oxidoreductase FAD-binding subunit YagSPeriplasmicTAT (piggyback) The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm (data not shown). Tat substrates are matured in the cytoplasm prior to their translocation. The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm (data not shown). PMID: 19368556PMID: 19368556 PMID: 17218314PeriplasmicGN/APeriplasm Membrane anchoredTAT (piggyback)noyesN160.129CYT0-0.200913-00NiCytoplasmic915
YNFE_ECOLIP77374
ynfE
b1587 JW1579 periplasmic peripheral IM protein (TAT through IM), Putative dimethyl sulfoxide reductase chain YnfE Peripheral inner membrane protein facing the periplasmTAT secretion system. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592PMID: 17218314 PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/ACell membrane PeriplasmicTATyesyesN280.177SpI424.96127-00Yn19-37c42/43oCytoplasmicMembrane921
YDHX_ECOLIP77375
ydhX
b1671 JW5271 periplasmic protein (TAT-SEC through IM), Ferredoxin-like YdhXPeriplasmicTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTAT,SECyesyesN280.293SpI276.80968-10Yn8-19c27/28oUnknown4
YAGR_ECOLIP77489
yagR
b0284 JW0278 periplasmic protein (TAT via piggyback through IM), Aldehyde oxidoreductase molybdenum-binding subunit YagRPeriplasmicTAT (piggyback) The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm. Tat substrates are matured in the cytoplasm prior to their translocation.PMID: 19368556PeriplasmicGN/APeriplasm PeriplasmicTAT (piggyback)noyesN600.121CYT0-0.200913-00NiCytoplasmicMembrane927
YAHJ_ECOLIP77554
yahJ
b0324 JW0316 periplasmic protein (TAT-SEC through IM), YahJPeriplasmicTAT secretion system.PMID: 17218314PeriplasmicGN/APeriplasmicTAT,SECnoyesY340.674SpI3311.0447-00Yn18-28c33/34oUnknown412
YNFF_ECOLIP77783
ynfF
b1588 JW5260 periplasmic peripheral IM protein (TAT-SEC through IM), Probable dimethyl sulfoxide reductase chain YnfF Peripheral inner membrane protein facing the periplasmVerified Tat substrate: YnfF has a Tat-only (Class II) predicted 45 aa signal peptide (Tullman-Ercek, 2007). The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.; DmsA paralog.So far, hydroxy pyridine N-oxide is the The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592PMID: 17218314 PMID: 19415239 PMID: 14522592Peripheral inner membrane protein facing the periplasmF2N/ACell membrane PeriplasmicTAT,SECyesyesN460.406SpI4511.1244-00Yn24-37c45/46oCytoplasmicMembrane99