Type III secretion proteins (T3SS) in E.coli EPEC (O127:H6) (lee locus)

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Protein name
E.coliEPEC
(O127:H6)
Previous names
E.coliEPEC
(O127:H6)
Accession
(UniProt)
E.coliEPEC
(O127:H6)
Common
nomenclature
name
Gene name
E.coliEPEC
(O127:H6)
Molecular
weight (Da)
Protein
Length
(amino acids)
Structural
domains
Position Stochiometry Subunit size (kDa) Post-Translational
Modifications
(PTMs)
Secretion
Mechanism
Effect of deletion mutation
on secretion
Effect on pedestal formation
/virulence
Topology Class
(UniProt)
Functional
Domains
(PFAM)
Known
Interactions
between T3S
components
Known Function References
- -
SepL / B7UM95 SctW sepL 39.988 351 3 helical domains Affinity switch, Gatekeeper - - - T3S Translocators not secreted, Effectors oversecreted - Cytoplasm (weak interaction IM) PF07201 CesT 29, 30, 39, 50, 66, 67, 71, EscV 33, 34, Secreted proteins and their chaperones, SepD Receptor at the membrane for translocation; Once absent the translocation switches to effectors PMID: 12904549, PMID: 15186412, PMID: 15784556, PMID: 18673458, PMID: 26457522
EscV SepA B7UMA7 SctV escV 75.155 675 8 TM; large cytoplasmic domain Major component, external to channel 9 676.8 - Sec - - Inner Membrane PF00771 EscJ 41, 42, 43, Secreted proteins and their chaperones, SepL 8, 29, 30, 31, 32 Receptor at the membrane for translocation; Translocation channel PMID: 14985343, PMID: 15130117
EscU / B7UMB8 SctU escU 39.177 345 3 TM; large cytoplasmic domain Minor component, external to channel 1 to 2 39.2-78.4 cleaved at NPTH motif Sec - - Inner Membrane PF01312 EscI 44, 45, 46, 47, EscP 37, 38, EscR 29 Export apparatus; Switch mechanism from early to middle substrates PMID: 12904549, PMID: 15060055, PMID: 18451864
EscT / B7UMB9 SctT escT 28.745 258 5 TM Translocase channel 1 28.7 - Sec - - Inner Membrane PF01311 EscR 29, EscS 29, EscT 35 Export apparatus PMID: 15060055
EscS / B7UMC0 SctS escS 9.921 89 2 TM Translocase channel 1 9.9 - Sec - - Inner Membrane PF01313 EscR 29, EscT 35 Export apparatus PMID: 12904549
EscR / B7UMC1 SctR escR 24.382 217 4 TM Translocase channel 5 121.5 - Sec - - Inner Membrane PF00813 EscI 44, 45, 46, 47, EscR 29, EscS 29, EscT 35, EscU 29, 35, 36 Export apparatus PMID: 12904549
EscP / B7UMA4 SctP orf16 16.579 138 C-terminal conserved T3S4 domain (type III secretion and substrate specificity switch) that interacts with SctU C-terminus Molecular ruler - - - T3S Less translocators Not affected Secretory protein PF06523 CesT EscF EscI EscU Orf16 Ruler for filament length; Switch mechanism from early to middle substrates PMID: 15757514, PMID: 15773978
EscN SepB B7UMA6 SctN escN 48.858 446 cytoplasmic; residues 103–371 contain a mixed α, β Rossmann fold; highly helical Hexameric ring-structure ATPase 6 293.1 - No - - Cytoplasm (weak interaction IM) PF00006 EscL 37, 40, EscO 50, EscQ 50, EscV 33, 34, Secreted proteins and their chaperones ATPase (walker box motif). Forms hexameric complex PMID: 12904549, PMID: 14617638
EscL / B7UMC2 SctL orf5 27.18 231 helical protein External stator connecting ATPase and Cytoplasmic ring 12 326.2 - No - - Cytoplasm (weak interaction IM) EscN 29, 39, EscO 50, EscQ 50, EscV 33, 34, EspA 29, 32, 40, 54, 55 Negative regulator of ATPase PMID: 15757514, PMID: 19028682
EscK / B7UMC3 SctK orf4 23.465 199 - Connector of Pods with SctD 6 140.8 - No - - Cytoplasm (weak interaction IM) EscD 50, EscQ 50 Connector of Pods with SctD PMID: 15757514
EscJ / B7UMB1 SctJ escJ 20.757 190 1 large TM, periplasm-facing lipoprotein Lipoprotein ring component 24 498.2 15931226" class="ref">PMID: 15931226<, Palmitoylated at C19 (by analogy to PrgK PF01514 EscD 50, EscI 44, 45, 46, 47, EscJ 41, 42, 43, EscV 33, 34 IM ring PMID: 10984518, PMID: 15001355, PMID: 15931226
EscI / B7UMB0 SctI rorf8 15.258 142 peripolasmic protein Inner rod 3 45.9 - T3S - - Periplasm PF09392 EscC 50, 51, EscI 44, 45, 46, 47, EscP 37, 38, EscR 29, EscT 35, EscU 29, 35, 36, EtgA 59, 60 Inner rod protein assists in secretin insertion into OM PMID: 10921870, PMID: 11169106, PMID: 12754250, PMID: 15528446
EscF / B7UM90 SctF escF 8.127 73 helical protein Needle component 120 974 - T3S - - Secretory protein PF09392 EscE 37, EscF 48, EscG 50, EscP 37, 38 Major Needle component PMID: 16585741
EspD / B7UM93 SctE espD 39.499 380 2 TM, mainly hydrophylic Translocon pore protein 8 316 - T3S Not affected - Host plasma membrane PF04888 CesD2, EspA 29, 32, 40, 54, 55, EspB 29, 52, 53, EspC, EspD 29, 49 Translocator, pore forming protein PMID: 11349076, PMID: 12904549
EscD / B7UM96 SctD escD 45.314 406 1 TM Major IM ring component 24 1087.2 - Sec - - Inner Membrane EscC 50, 51, EscJ 41, 42, 43, EscK 37, EscQ 50 EscD and EscJ form ring-like structure in inner membrane; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling. PMID: 27818950
EscC / B7UMB3 SctC escC 56.226 512 N-terminal periplasmic domain; secretin family protein Secretin 12 to 14 674.4 - Sec - - Outer Membrane PF00263, PF03958 EscD 50, EscI 44, 45, 46, 47, EscO 50 T3SS basal body and forms OM ring PMID: 12675813, PMID: 27818950
EspB eaeB Q05129 SctB espB 33.141 321 mainly hydrophylic and helical Translocon pore protein 8 264.8 - T3S Not affected - Host plasma membrane PD454109 CesAB 2, 29, 37, 54, 55, CesD 3, 29, EscN 29, 39, EscV 33, 34, EspA 29, 32, 40, 54, 55, EspD 29, 49, SepL 8, 29, 30, 31, 32 Translocator, pore forming protein. Binds to myosins that interact with actin filaments and mediate essential processes (e.g.microvillus formation ,phagocytosis). Inhibits the interaction of myosins with actin PMID: 18078690. PMID: 12904549, PMID: 18078690, PMID: 20477867
EspA / B7UM94 SctA espA 20.469 192 mainly helical Filament protein 28 per turn 574 - T3S Not affected - Secretory protein PF0343 CesAB 2, 29, 37, 54, 55, EscL 37, 40, EscN 29, 39, EscV 33, 34, EspB 29, 52, 53, EspD 29, 49, SepL 8, 29, 30, 31, 32 Translocator. Half-flagellin model; dimerizes before polymerizes; lacks D0 and D3 domains. PMID: 12904549, PMID: 15619638, PMID: 15784556, PMID: 18178741, PMID: 19028682
- / B7UMC9 - rorf1 31.054 272 - - - - - - unknown + - - SdiA-regulated domain (NHLrepeats). Unknown Function. -
EspG rorf2 B7UMC8 - espG 43.919 398 cytoplasmic Effector more than injectisomes - - T3S Not affected + Host Cytoplasm PF06872 CesT 29, 30, 39, 50, 66, 67, 71 Translocated effector. Disassemble microtubules to induce RhoGTPase-mediated cytokeletal rearrangements that promote bacterial attachment, entry, or movement within the cytoplasm PMID: 15972534. Activating the host cell protease calpain. PMID: 11349072, PMID: 15757514, PMID: 15972534, PMID: 27818950
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