E. coli BL21(DE3) proteome

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Nucleoid Cytoplasmic Peripheral inner membrane protein facing the cytoplasm Integral inner membrane Ribosomal Inner membrane lipoprotein Peripheral inner membrane protein facing the periplasm Periplasmic Peripheral outer membrane protein facing the periplasm Outer membrane β-barrel protein Peripheral outer membrane protein facing the extra-cellular space Extra-cellular Outer membrane lipoprotein

Protein Information Subcellular Localization Targeting/Export System
Protein ID /
Accession #
(UniProt)
Gene Name
(UniProt)
Protein Name
(STEPdb)
Order
Locus Name
Full name
(STEPdb)
Symbol
(STEPdb)
Topology Class
UniProt
First Level
Targeting
Chaperoning
First Level
Secretion
System
Second Level
Targeting
Chaperoning
Second Level
Secretion
System
K-12 Core
Proteome
(yes/no)
K-12 Basic
Proteome
(yes/no)
A0A140N1P7_ECOBD
A0A140N1P7
yidA
Cof-like hydrolase (Predicted hydrolase) B21_03524 ECBD_0006 ECD_03580 Cytoplasmic A Cytoplasm, Inner Membrane, Membrane SEC SRP - - no,yes yes
A0A140N1Q5_ECOBD
A0A140N1Q5
ibpA
Heat shock chaperone (Heat shock protein Hsp20) B21_03514 ECBD_0016 ECD_03570 Cytoplasmic A Cytoplasm, Inner Membrane, nucleoid - - - - no,yes yes
A0A140N1Q9_ECOBD
A0A140N1Q9
yidG
Predicted inner membrane protein (Putative uncharacterized protein) B21_03502 ECBD_0027 ECD_03559 Integral Inner Membrane, cytoplasmic side B Inner Membrane SRP SEC - - no yes
A0A140N1R7_ECOBD
A0A140N1R7
uhpT
Phosphoglycerate transporter (Sugar phosphate antiporter) B21_03492 ECBD_0037 ECD_03550 Integral Inner Membrane, cytoplasmic side B Inner Membrane SEC,SRP SEC - - no,yes yes
A0A140N1S6_ECOBD
A0A140N1S6

Putative uncharacterized protein ECBD_0047 Cytoplasmic A Inner Membrane - - - - no yes
A0A140N1T3_ECOBD
A0A140N1T3

Transposase IS3/IS911 family protein ECBD_0058 Cytoplasmic A Secretory protein - - - - no no,yes
A0A140N1U2_ECOBD
A0A140N1U2
yicI
Glycoside hydrolase family 31 (Predicted alpha-glucosidase) B21_03466 ECBD_0068 ECD_03514 Cytoplasmic A Cell inner membrane, Cytoplasm (weak interaction IM) SEC SRP - - no yes
A0A140N1V2_ECOBD
A0A140N1V2
ligB
yicF
DNA ligase B (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+] B) B21_03456 ECBD_0078 ECD_03504 Cytoplasmic A Cell inner membrane, Cytoplasm (weak interaction IM) SEC SRP - - no,yes yes
A0A140N1W0_ECOBD
A0A140N1W0
radC
yicR
UPF0758 protein yicR B21_03447 ECBD_0088 ECD_03495 Cytoplasmic A Cytoplasm, Cytoplasm (weak interaction IM), Note=Restricted to the nucleoid region., nucleoid SEC SRP - - no,yes yes
A0A140N1Y4_ECOBD
A0A140N1Y4
tdh
L-threonine 3-dehydrogenase (EC 1.1.1.103) B21_03425 ECBD_0109 ECD_03474 Cytoplasmic A Cell inner membrane, Cytoplasm, Inner Membrane, Note=Restricted to the nucleoid region., nucleoid SEC SEC,SRP - - no,yes yes
A0A140N1Z5_ECOBD
A0A140N1Z5
yibK
trmL
Predicted rRNA methylase (RNA methyltransferase, TrmH family, group 2) B21_03415 ECBD_0119 ECD_03464 Cytoplasmic A Cytoplasm, Periplasm - - - - no,yes yes
A0A140N201_ECOBD
A0A140N201
mtlA
Fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components (PTS system, mannitol-specific IIC subunit) B21_03405 ECBD_0129 ECD_03454 Integral Inner Membrane, cytoplasmic side B Cytoplasm, Secretory protein SRP SEC - - no,yes yes
A0A140N210_ECOBD
A0A140N210
selB
Selenocysteine-specific translation elongation factor (Selenocysteinyl-tRNA-specific translation factor) B21_03396 ECBD_0140 ECD_03445 Cytoplasmic A Cell inner membrane, Cytoplasm, Host plasma membrane SEC SRP - - no,yes yes
A0A140N220_ECOBD
A0A140N220
ybl147
Putative regulatory protein (Transcriptional regulator, AraC family) B21_03387 ECBD_0150 ECD_03436 Cytoplasmic A Cytoplasm, Inner Membrane - - - - no no,yes
A0A140N231_ECOBD
A0A140N231
yiaJ
Predicted DNA-binding transcriptional repressor (Transcriptional regulator, IclR family) B21_03377 ECBD_0160 ECD_03426 Nucleoid N Cell inner membrane, Outer Membrane SEC SRP - - no no,yes
A0A140N239_ECOBD
A0A140N239
yiaB
Conserved inner membrane protein (YiaAB two helix domain protein) B21_03366 ECBD_0171 ECD_03415 Integral Inner Membrane, cytoplasmic side B Host plasma membrane SEC,SRP SEC - - no yes
A0A140N249_ECOBD
A0A140N249
yiaG
Predicted transcriptional regulator (Transcriptional regulator, XRE family) B21_03356 ECBD_0181 ECD_03405 Nucleoid N Cell membrane, Secretory protein SEC - - - no,yes yes
A0A140N258_ECOBD
A0A140N258
eptB
yhjW
Predicted metal dependent hydrolase (Sulfatase) B21_03347 ECBD_0191 ECD_03396 Integral Inner Membrane, cytoplasmic side B Cytoplasm SRP SEC - - no,yes yes
A0A140N267_ECOBD
A0A140N267
bcsG
yhjU
Cellulose synthase operon protein YhjU (Predicted inner membrane protein) B21_03339 ECBD_0201 ECD_03386 Integral Inner Membrane, cytoplasmic side B Cell inner membrane, Cytoplasm, Host Cytoplasm SEC,SRP SEC - - no yes
A0A140N277_ECOBD
A0A140N277
dctA
C4-dicarboxylate transport protein (Sodium:dicarboxylate symporter) B21_03329 ECBD_0211 ECD_03376 Integral Inner Membrane, cytoplasmic side B Cytoplasm, Note=Restricted to the nucleoid region., nucleoid SRP SEC - - no yes
Records 1 to 20 of 4159