Type III secretion proteins (T3SS) in E.coli EPEC (O127:H6) (lee locus)

Protein name E.coliEPEC (O127:H6)	Previous names E.coliEPEC (O127:H6)	Accession (UniProt) E.coliEPEC (O127:H6)	Common nomenclature name	Gene name E.coliEPEC (O127:H6)	Molecular weight (Da)	Protein Length (amino acids)	Structural domains	Position	Stochiometry	Subunit size (kDa)	Post-Translational Modifications (PTMs)	Secretion Mechanism	Effect of deletion mutation on secretion	Effect on pedestal formation /virulence	Topology Class (UniProt)	Functional Domains (PFAM)	Known Interactions between T3S components	Known Function	References
Search.. Close CesAB CesD CesD2 CesF CesT EscC EscD EscE EscF EscG EscI EscJ EscK EscL EscN EscO EscP EscQ EscR EscS EscT EscU EscV EspA EspB EspD EspF EspG EspH EspZ/SepZ EtgA GrlA GrlR Intimin Ler Map Mpc/CesL SepD SepL Tir None	Search.. Close eaeA eaeB Hp90 orf1 orf18 orfd4 orfU rorf10 rorf2 SepA SepB SepQ None	Search.. Close B7UM88 B7UM89 B7UM90 B7UM91 B7UM93 B7UM94 B7UM95 B7UM96 B7UM99 B7UMA0 B7UMA1 B7UMA2 B7UMA3 B7UMA4 B7UMA5 B7UMA6 B7UMA7 B7UMA8 B7UMA9 B7UMB0 B7UMB1 B7UMB2 B7UMB3 B7UMB4 B7UMB5 B7UMB6 B7UMB7 B7UMB8 B7UMB9 B7UMC0 B7UMC1 B7UMC2 B7UMC3 B7UMC4 B7UMC5 B7UMC6 B7UMC8 B7UMC9 P19809 P21244 Q05129	Search.. Close SctA SctB SctC SctD SctE SctF SctI SctJ SctK SctL SctN SctP SctR SctS SctT SctU SctV SctW None	Search.. Close cesD cesF cesT eae escC escD escF escJ escN escQ escR escS escT escU escV espA espB espD espF espG espH espZ/sepZ ler orf10 orf11 orf12 orf15 orf16 orf19 orf2 orf27 orf29 orf3 orf4 orf5 rorf1 rorf3 rorf6 rorf8 sepL tir	-	-	Search.. Close 1 large TM 1 TM 2 TM 2TM 3 helical domains 3 TM; large cytoplasmic domain 4 TM 5 TM 8 TM; large cytoplasmic domain C-terminal conserved T3S4 domain (type III secretion and substrate specificity switch) that interacts with SctU C-terminus cytoplasmic cytoplasmic; residues 103–371 contain a mixed α helical protein β Rossmann fold; highly helical mainly helical mainly hydrophylic mainly hydrophylic and helical N-terminal periplasmic domain; secretin family protein periplasm-facing lipoprotein peripolasmic peripolasmic protein secreted None	Search.. Close Affinity switch Attaching and effacing protein Central stalk Chaperone (effectors) Chaperone (translocators) Chaperone of EscF Component of 6 Pod assembly Connector of Pods with SctD Effector External stator connecting ATPase and Cytoplasmic ring external to channel Filament protein Gatekeeper Global Regulator Hexameric ring-structure ATPase Inner rod inserting in ATPase ring Lipoprotein ring component Major component Major IM ring component Minor component Molecular ruler Muramidase Needle component Negative regulator Positive regulator Secretin Subunit 1 of SctW chaperone heterodimer Subunit 2 of SctW chaperone heterodimer Translocase channel Translocon pore protein None	Search.. Close 1 1 per SctW 1 to 2 12 12 to 14 120 24 28 per turn 3 5 6 8 9 more than injectisomes None	Search.. Close 1087.2 121.5 14.7 140.8 264.8 28.7 293.1 316 326.2 39.2-78.4 419.6 45.9 498.2 574 674.4 676.8 9.9 974 None	Search.. Close 15220932" class="ref">PMID: 15220932< 15931226" class="ref">PMID: 15931226< 16954405" class="ref">PMID: 16954405< a>) a>. a>; pS436 cleaved at NPTH motif Palmitoylated at C19 (by analogy to PrgK pS437 by host kinases pubmed pY474 by host kinases; pY454 by host kinases www.ncbi.nlm.nih.gov None	Search.. Close Lipoprotein No Sec T3S None	Search.. Close - Less EspD Less translocators No EspA, less EspB No expression Not affected Translocators not secreted, Effectors oversecreted unknown	Search.. Close - + Not affected	Search.. Close Bacterial outer membrane Cytoplasm Cytoplasm (weak interaction IM) Host Cytoplasm Host cytoplasmic membrane Host plasma membrane Inner Membrane Outer Membrane Peptidoglycan Periplasm Secretory protein None	Search.. Close PD454109 PF00006 PF00263 PF00771 PF00813 PF00816 PF01311 PF01312 PF01313 PF01464 PF01514 PF03278 PF0343 PF03549 PF03958 PF04806 PF04888 PF05932 PF06523 PF06704 PF06872 PF07201 PF07489 PF07490 PF07720 PF09392 PF09621 None	Search.. Close CesAB CesD CesD2 CesF CesL CesT CesT EscF EscI EscU Orf16 EscC EscD EscE EscF EscG EscI EscJ EscK EscL EscN EscO EscP EscQ EscR EscS EscT EscU EscV EspA EspB EspC EspD EspF EspG EspH EspZ EtgA GapA GrlA GrlR HNS Ler Map Mcp NleA NleF NleG NleH NleH2 Secreted proteins and their chaperones SepD SepL StpA Tir None	Search.. Close Adhesin on the bacterial membrane ATPase (walker box motif). Forms hexameric complex central stalk attached to C-terminus of EscV; stabilizes the EscN hexamer and stimulates its ATPase activity Chaperone for EspA and EspB Chaperone for EspB and EspD Chaperone for EspF Chaperone of EspD. Homologous LcrR will bind YopB. Chaperone of SepL together with CesL and regulator of switch mechanism Chaperone of SepL together with SepD and regulator of switch mechanism Chaperone of Tir and Map Connector of Pods with SctD EscD and EscJ form ring-like structure in inner membrane; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling. Export apparatus Export apparatus; Switch mechanism from early to middle substrates Forms ring within basal body IM ring Inner rod protein assists in secretin insertion into OM Major Needle component Mitochondrial dysfunction; Induces transient filopodia formation Muramidase that degrades peptidoglycan N-terminal and C-terminal domains remain intracellular and interact with numerous signalling, adapter and cytoskeletal proteins. Function dependent on its phosphorylation sites, a GTPase activating protein (GAP)-like motif and a polyproline region. Phosphorylation affects oligomerization. Negative regulator of ATPase Negative regulator TTSS; avoiding GrlA binding to the LEE1 promoter and thus repressing ler expression One of the chaperones of EscF Positive regulator of T3SS Positive regulator TTSS Receptor at the membrane for translocation; Once absent the translocation switches to effectors Receptor at the membrane for translocation; Translocation channel Ruler for filament length; Switch mechanism from early to middle substrates SdiA-regulated domain (NHLrepeats). Unknown Function. T3SS basal body and forms OM ring Translocated effector Translocated effector. Disassemble microtubules to induce RhoGTPase-mediated cytokeletal rearrangements that promote bacterial attachment, entry, or movement within the cytoplasm PMID: 15972534. Activating the host cell protease calpain. Translocated effector. Mitochondrial dysfunction. Microvilli effacement. TJ disruption, apoptosis. Epithelial transporter inhibition. Antiphagocytosis. Membrane remodeling. Actin-pedestal maturation. Nucleolus. Translocated effector; Contributes to host cell survival mechanisms Translocator, pore forming protein Translocator, pore forming protein. Binds to myosins that interact with actin filaments and mediate essential processes (e.g.microvillus formation ,phagocytosis). Inhibits the interaction of myosins with actin PMID: 18078690. Translocator. Half-flagellin model; dimerizes before polymerizes; lacks D0 and D3 domains.
SepL	-	B7UM95	SctW	sepL	39	351	3 helical domains	Affinity switch, Gatekeeper	-	-	-	T3S	Translocators not secreted, Effectors oversecreted	-	Cytoplasm (weak interaction IM)	PF07201	CesT 29, 30, 39, 50, 66, 67, 71, EscV 33, 34, Secreted proteins and their chaperones, SepD	Receptor at the membrane for translocation; Once absent the translocation switches to effectors	PMID: 12904549, PMID: 15186412, PMID: 15784556, PMID: 18673458, PMID: 26457522
EscV	SepA	B7UMA7	SctV	escV	75	675	8 TM; large cytoplasmic domain	Major component, external to channel	9	676.8	-	Sec	-	-	Inner Membrane	PF00771	EscJ 41, 42, 43, Secreted proteins and their chaperones, SepL 8, 29, 30, 31, 32	Receptor at the membrane for translocation; Translocation channel	PMID: 14985343, PMID: 15130117
EscU	-	B7UMB8	SctU	escU	39	345	3 TM; large cytoplasmic domain	Minor component, external to channel	1 to 2	39.2-78.4	cleaved at NPTH motif	Sec	-	-	Inner Membrane	PF01312	EscI 44, 45, 46, 47, EscP 37, 38, EscR 29	Export apparatus; Switch mechanism from early to middle substrates	PMID: 12904549, PMID: 15060055, PMID: 18451864
EscT	-	B7UMB9	SctT	escT	28	258	5 TM	Translocase channel	1	28.7	-	Sec	-	-	Inner Membrane	PF01311	EscR 29, EscS 29, EscT 35	Export apparatus	PMID: 15060055
EscS	-	B7UMC0	SctS	escS	9	89	2 TM	Translocase channel	1	9.9	-	Sec	-	-	Inner Membrane	PF01313	EscR 29, EscT 35	Export apparatus	PMID: 12904549
EscR	-	B7UMC1	SctR	escR	24	217	4 TM	Translocase channel	5	121.5	-	Sec	-	-	Inner Membrane	PF00813	EscI 44, 45, 46, 47, EscR 29, EscS 29, EscT 35, EscU 29, 35, 36	Export apparatus	PMID: 12904549
EscP	-	B7UMA4	SctP	orf16	16	138	C-terminal conserved T3S4 domain (type III secretion and substrate specificity switch) that interacts with SctU C-terminus	Molecular ruler	-	-	-	T3S	Less translocators	Not affected	Secretory protein	PF06523	CesT EscF EscI EscU Orf16	Ruler for filament length; Switch mechanism from early to middle substrates	PMID: 15757514, PMID: 15773978
EscN	SepB	B7UMA6	SctN	escN	48	446	cytoplasmic; residues 103–371 contain a mixed α, β Rossmann fold; highly helical	Hexameric ring-structure ATPase	6	293.1	-	No	-	-	Cytoplasm (weak interaction IM)	PF00006	EscL 37, 40, EscO 50, EscQ 50, EscV 33, 34, Secreted proteins and their chaperones	ATPase (walker box motif). Forms hexameric complex	PMID: 12904549, PMID: 14617638
EscL	-	B7UMC2	SctL	orf5	27	231	helical protein	External stator connecting ATPase and Cytoplasmic ring	12	326.2	-	No	-	-	Cytoplasm (weak interaction IM)		EscN 29, 39, EscO 50, EscQ 50, EscV 33, 34, EspA 29, 32, 40, 54, 55	Negative regulator of ATPase	PMID: 15757514, PMID: 19028682
EscK	-	B7UMC3	SctK	orf4	23	199	-	Connector of Pods with SctD	6	140.8	-	No	-	-	Cytoplasm (weak interaction IM)		EscD 50, EscQ 50	Connector of Pods with SctD	PMID: 15757514
EscJ	-	B7UMB1	SctJ	escJ	20	190	1 large TM, periplasm-facing lipoprotein	Lipoprotein ring component	24	498.2	15931226" class="ref">PMID: 15931226<, Palmitoylated at C19 (by analogy to PrgK PF01514	EscD 50, EscI 44, 45, 46, 47, EscJ 41, 42, 43, EscV 33, 34	IM ring	PMID: 10984518, PMID: 15001355, PMID: 15931226
EscI	-	B7UMB0	SctI	rorf8	15	142	peripolasmic protein	Inner rod	3	45.9	-	T3S	-	-	Periplasm	PF09392	EscC 50, 51, EscI 44, 45, 46, 47, EscP 37, 38, EscR 29, EscT 35, EscU 29, 35, 36, EtgA 59, 60	Inner rod protein assists in secretin insertion into OM	PMID: 10921870, PMID: 11169106, PMID: 12754250, PMID: 15528446
EscF	-	B7UM90	SctF	escF	8	73	helical protein	Needle component	120	974	-	T3S	-	-	Secretory protein	PF09392	EscE 37, EscF 48, EscG 50, EscP 37, 38	Major Needle component	PMID: 16585741
EspD	-	B7UM93	SctE	espD	39	380	2 TM, mainly hydrophylic	Translocon pore protein	8	316	-	T3S	Not affected	-	Host plasma membrane	PF04888	CesD2, EspA 29, 32, 40, 54, 55, EspB 29, 52, 53, EspC, EspD 29, 49	Translocator, pore forming protein	PMID: 11349076, PMID: 12904549
EscD	-	B7UM96	SctD	escD	45	406	1 TM	Major IM ring component	24	1087.2	-	Sec	-	-	Inner Membrane		EscC 50, 51, EscJ 41, 42, 43, EscK 37, EscQ 50	EscD and EscJ form ring-like structure in inner membrane; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling.	PMID: 27818950
EscC	-	B7UMB3	SctC	escC	56	512	N-terminal periplasmic domain; secretin family protein	Secretin	12 to 14	674.4	-	Sec	-	-	Outer Membrane	PF00263, PF03958	EscD 50, EscI 44, 45, 46, 47, EscO 50	T3SS basal body and forms OM ring	PMID: 12675813, PMID: 27818950
EspB	eaeB	Q05129	SctB	espB	33	321	mainly hydrophylic and helical	Translocon pore protein	8	264.8	-	T3S	Not affected	-	Host plasma membrane	PD454109	CesAB 2, 29, 37, 54, 55, CesD 3, 29, EscN 29, 39, EscV 33, 34, EspA 29, 32, 40, 54, 55, EspD 29, 49, SepL 8, 29, 30, 31, 32	Translocator, pore forming protein. Binds to myosins that interact with actin filaments and mediate essential processes (e.g.microvillus formation ,phagocytosis). Inhibits the interaction of myosins with actin PMID: 18078690.	PMID: 12904549, PMID: 18078690, PMID: 20477867
EspA	-	B7UM94	SctA	espA	20	192	mainly helical	Filament protein	28 per turn	574	-	T3S	Not affected	-	Secretory protein	PF0343	CesAB 2, 29, 37, 54, 55, EscL 37, 40, EscN 29, 39, EscV 33, 34, EspB 29, 52, 53, EspD 29, 49, SepL 8, 29, 30, 31, 32	Translocator. Half-flagellin model; dimerizes before polymerizes; lacks D0 and D3 domains.	PMID: 12904549, PMID: 15619638, PMID: 15784556, PMID: 18178741, PMID: 19028682
-	-	B7UMC9	-	rorf1	31	272	-	-	-	-	-	-	unknown	+	-		-	SdiA-regulated domain (NHLrepeats). Unknown Function.	-
EspG	rorf2	B7UMC8	-	espG	43	398	cytoplasmic	Effector	more than injectisomes	-	-	T3S	Not affected	+	Host Cytoplasm	PF06872	CesT 29, 30, 39, 50, 66, 67, 71	Translocated effector. Disassemble microtubules to induce RhoGTPase-mediated cytokeletal rearrangements that promote bacterial attachment, entry, or movement within the cytoplasm PMID: 15972534. Activating the host cell protease calpain.	PMID: 11349072, PMID: 15757514, PMID: 15972534, PMID: 27818950
Previous Records 1 to 20 of 41 Next 20 30 50