Chaperonome of E.coli K-12 (MG1655)

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Nucleoid Cytoplasmic Peripheral inner membrane protein facing the cytoplasm Integral inner membrane Ribosomal Inner membrane lipoprotein Peripheral inner membrane protein facing the periplasm Periplasmic Peripheral outer membrane protein facing the periplasm Outer membrane β-barrel protein Peripheral outer membrane protein facing the extra-cellular space Extra-cellular Outer membrane lipoprotein

Family Protein ID /
Accession #
(UniProt)
Gene Name
(UniProt)
Protein Name
(UniProt)
Protein Name
(STEPdb)
Co-factors Type of activity ATP-requirement Clients Function Symbol
(STEPdb)
Topology Class
Full name
(STEPdb)
Concentration (𝜇M)
(data from
Schmidt 2016):
Minimal medium
+ Glucose
Concentration (𝜇M)
(data from
Schmidt 2016):
LB
Binding
affinity (𝜇M)
to
preproteins
Binding
affinity (𝜇M)
to
mature domains
References
- -
Chaperones in the cell envelope BEPA_ECOLI
P66948
bepA Beta-barrel assembly-enhancing protease (EC 3.4.-.-) periplasmic protein (SEC through IM), Beta-barrel assembly-enhancing protease BepA - - No Protein Assembles outer membrane proteins (chaperone) and degrades compromised outer membrane proteins (metalloprotease) G Periplasmic 0.29333559525903113 0.23219800476973335 - - Narita 2013
Chaperones in the cell envelope DPPA_ECOLI
P23847
dppA Periplasmic dipeptide transport protein (Dipeptide-binding protein) (DBP) periplasmic protein (SEC through IM), dipeptide transport DppA - - No Protein Overexpressed in bacterial strain with enhanced periplasmic folding capacity G Periplasmic 8.200861599022275 4.111257047619937 - - Lennon 2015
Chaperones in the cell envelope LOLB_ECOLI
P61320
lolB Outer-membrane lipoprotein LolB OM lipoprotein (SEC through IM), inserted in OM by LOL), Outer-LolB - - - Protein Incorporates lipoproteins, released from LolA, into the outer membrane I Outer Membrane Lipoprotein 0.42511454297849405 0.42400002530552916 - - Okuda 2009
Chaperones in the cell envelope OPPA_ECOLI
P23843
oppA Periplasmic oligopeptide-binding protein periplasmic protein (SEC through IM), oligopeptide-binding protein OppA - - No Protein Interacts with unfolded/denatured proteins. Overexpressed in bacterial strain with enhanced periplasmic folding capacity G Periplasmic 9.556489584574127 11.098575003986056 - - Lennon 2015; Richarme 1997
Chaperones in the cell envelope FKBA_ECOLI
P45523
fkpA FKBP-type peptidyl-prolyl cis-trans isomerase FkpA (PPIase) (EC 5.2.1.8) (Rotamase) periplasmic protein (SEC through IM), FKBP-type peptidyl-prolyl cis-trans isomerase FkpA - - No Protein Assists protein folding in the cell envelope via its PPiase and chaperone activity G Periplasmic - - - - De Geyter 2016
Chaperones in the cell envelope BAMA_ECOLI
P0A940
bamA Outer membrane protein assembly factor BamA (Omp85) OM b-barrel protein (SEC through IM, inserted in OM by BAM), assembly factor BamA - - - Protein POTRA domains of BamA recruit and assist in the folding of outer membrane protein H Outer Membrane b-barrel protein 3.36904641231776 5.019756088591476 - - Kim 2007; Knowles 2008; Sánchez-Pulido 2013;; Zhang 2011
Chaperones in the cell envelope SKP_ECOLI
P0AEU7
skp Chaperone protein Skp (DNA-binding 17 kDa protein) (Histone-like protein HLP-1) (Seventeen kilodalton protein) periplasmic protein (SEC through IM), Chaperone Skp - - No Protein Assists outer membrane proteins in crossing the periplasm and folding in the outer membrane G Periplasmic 13.655994566173096 22.00142887217872 - - Bulieris 2003; Chen 1996; Harms 2001; Schaefer 1999; de Cock 1999
Chaperones in the cell envelope LOLA_ECOLI
P61316
lolA Outer-membrane lipoprotein carrier protein (P20) periplasmic protein (SEC through IM), Outer-carrier LolA - - No Protein Translocates lipoproteins from the inner to the outer membrane G Periplasmic 1.1892136083287914 2.299987887284814 - - Okuda 2009
Chaperones in the cell envelope DEGP_ECOLI
P0C0V0
degP Periplasmic serine endoprotease DegP (EC 3.4.21.107) (Heat shock protein DegP) (Protease Do) periplasmic protein (SEC through IM), serine endoprotease DegP - - - Protein Chaperone at low temperatures: folds outer membrane proteins, peptidase at higher temperatures: degrades denatured/misfolded/unfolded proteins in the periplasm G Periplasmic 5.542881289038737 4.294204462871657 - - Krojer 2008; Spiess 1999
Chaperones in the cell envelope SURA_ECOLI
P0ABZ6
surA Chaperone SurA (Peptidyl-prolyl cis-trans isomerase SurA) (PPIase SurA) (EC 5.2.1.8) (Rotamase SurA) (Survival protein A) periplasmic protein (SEC through IM), Chaperone SurA - - No Protein Folds and assembles outer membrane proteins G Periplasmic - - - - Rouviere 1996
Chaperones in the cell envelope DJLA_ECOLI
P31680
djlA Co-chaperone protein DjlA (DnaJ-like protein DjlA) integral IM protein (SEC in IM), Co-chaperone DjlA - - - Protein Interacts with DnaK to induce the wcaABCDE operon B Integral Inner Membrane, cytoplasmic side 0.0016805942872791513 0.00005825310815277878 - - Chen 2001; Genevaux 2001; Kelley 1997
Chaperones in the cell envelope PPID_ECOLI
P0ADY1
ppiD Peptidyl-prolyl cis-trans isomerase D (PPIase D) (EC 5.2.1.8) (Rotamase D) integral IM protein (SEC in IM), Peptidyl-prolyl cis-trans isomerase D PpiD - - - Protein Accelerates protein folding in the cell envelope B Integral Inner Membrane, cytoplasmic side - - - - Antonoaea 2008
Chaperones in the cell envelope OSMY_ECOLI
P0AFH8
osmY Osmotically-inducible protein Y periplasmic & extra-cellular protein (SEC through IM), Osmotically-inducible Y OsmY - - No Protein Overexpressed in bacterial strain with enhanced periplasmic folding capacity G,X Periplasmic, Extra-cellular 17.41854580052776 8.408567259943974 - - Lennon 2015
Chaperones in the cell envelope KBAZ_ECOLI
P0C8K0
kbaZ D-tagatose-1,6-bisphosphate aldolase subunit KbaZ cytoplasmic protein, D-tagatose-1,6-bisphosphate aldolase subunit KbaZ - - - Protein Component of the tagatose-1,6-bisphosphate aldolase KbaYZ. KbaZ could have chaperone-like function on KbaY A Cytoplasmic 0.009713798840916638 0.015096996660577879 - - -
Chaperones in the cell envelope KDPC_ECOLI
P03961
kdpC Potassium-transporting ATPase KdpC subunit (ATP phosphohydrolase [potassium-transporting] C chain) (Potassium-binding and translocating subunit C) (Potassium-translocating ATPase C chain) integral IM protein (SEC in IM), Potassium-transporting ATPase KdpC subunit KdpC - - - Protein Increases ATP-binding affinity of KdpB in the high-affinity ATP-driven potassium transport (or Kdp) system B Integral Inner Membrane, cytoplasmic side - - - - Damnjanovic 2013; Irzik 2011; Kollmann 1993; Siebers 1988
Chaperones in the cell envelope IVY_ECOLI
P0AD59
ivy Inhibitor of vertebrate lysozyme periplasmic protein (SEC through IM), Inhibitor of vertebrate lysozyme Ivy - - No Protein Overexpressed in bacterial strain with enhanced periplasmic folding capacity G Periplasmic 2.8302150816539746 1.958958207565092 - - Lennon 2015
Chaperones in the cell envelope GATZ_ECOLI
P0C8J8
gatZ D-tagatose-1,6-bisphosphate aldolase subunit GatZ cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZ - - - Protein Component of the tagatose-1,6-bisphosphate aldolase GatYZ. GatZ could have chaperone-like function on GatY A,F1 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm 2.3465858995938875 20.457994947271537 - - Brinkkoetter 2002; Nobelmann 19996
Chaperones with role in secretion: Sec YIDC_ECOLI
P25714
yidC Membrane protein insertase YidC (Foldase YidC) (Inner membrane protein YidC) (Membrane integrase YidC) (Oxa1Ec) integral IM protein (SEC in IM), insertase YidC - - - Protein Helps LacY and MalF (Sec-dependent substrates) acquire their tertiary structure B Integral Inner Membrane, cytoplasmic side 1.397686083067173 3.3710709667564 - - Nagamori 2004; Wagner 2008
Chaperones with role in secretion: Sec SECA_ECOLI
P10408
secA Protein translocase subunit SecA cytoplasmic peripheral IM protein, translocase subunit SecA - - - Protein ATPase motor that drives preprotein translocation through the SecYEG channel with foldase and holdase activity A,F1 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm 1.1729045637525255 2.9786169750881073 1,96-3 3,15-7 Chatzi 2013; Eser 2003
Chaperones with role in secretion: Sec SECB_ECOLI
P0AG86
secB Protein-export protein SecB (Chaperone SecB) cytoplasmic peripheral IM protein, Protein-export SecB - Holdase - Protein Prevents preprotein folding/aggregation and guides preproteins to the membrane receptor SecA A,F1 Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm 27.79024830481191 46.34843575614366 0.0008 0.08 Baars 2006; Huang 2016
Records 1 to 20 of 116

The chaperonome list is available in the downloads section:
Chaperonome .xlsx format or Chaperonome .csv format

For more information: Loos et al., 2019.