Chaperonome of E.coli K-12 (MG1655)

Family	Protein ID / Accession # (UniProt)	Gene Name (UniProt)	Protein Name (UniProt)	Protein Name (STEPdb)	Co-factors	Type of activity	ATP-requirement	Clients	Function	Symbol (STEPdb)	Topology Class Full name (STEPdb)	Concentration (𝜇M) (data from Schmidt 2016): Minimal medium + Glucose	Concentration (𝜇M) (data from Schmidt 2016): LB	Binding affinity (𝜇M) to preproteins	Binding affinity (𝜇M) to mature domains	References
Search.. Close Chaperones in the cell envelope Chaperones with role in secretion: Sec Chaperones with role in secretion: Tat Co-factor Curli assembly Cytoplasmic chaperones DJ-1 superfamily Dsb family Fimbrial assembly Flagellar assembly Hsp100 Hsp33 Hsp40 Hsp60 Hsp70 Hsp90 Metal PPIase Proteins with adventitious chaperone-like activity RNA Small Hsps Stress Uncharacterized					Search.. Close DnaJ, GrpE, DjlA GroS HscA HscB Hsp70, ClpP YbeV, YbeS None	Search.. Close Disaggregase, unfoldase Disaggregase, unfoldase, Unfoldase Foldase Foldase, disaggregase Foldase, disaggregase, holdase Foldase, holdase Foldase/secretory chaperone Holdase Metal, disaggregase, Disaggregase Sigma factor regulator, disaggregase None	Search.. Close Negative regulation No Yes None	Search.. Close Co-factor metal Metal Protein RNA		Search.. Close A A,F1 A,F1,r A,N A,N,F1 A,r B F2 F2,G G G,X H I	Search.. Close Cytoplasmic Cytoplasmic, Nucleoid Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm Cytoplasmic, Peripheral plasma membrane protein facing the cytoplasm, Ribosomal Cytoplasmic, Ribosomal Integral Inner Membrane, cytoplasmic side Outer Membrane b-barrel protein Outer Membrane Lipoprotein Peripheral inner membrane protein facing the periplasm Peripheral inner membrane protein facing the periplasm, periplasmic Periplasmic Periplasmic, Extra-cellular	-	-	Search.. Close 0.0008 1,96-3 2-4 None	Search.. Close 0.08 3,15-7 4-14 None
Chaperones in the cell envelope	BEPA_ECOLI P66948	bepA	Beta-barrel assembly-enhancing protease (EC 3.4.-.-)	periplasmic protein (SEC through IM), Beta-barrel assembly-enhancing protease BepA	-	-	No	Protein	Assembles outer membrane proteins (chaperone) and degrades compromised outer membrane proteins (metalloprotease)	G	Periplasmic	0.29333559525903113	0.23219800476973335	-	-	Narita 2013
Chaperones in the cell envelope	DPPA_ECOLI P23847	dppA	Periplasmic dipeptide transport protein (Dipeptide-binding protein) (DBP)	periplasmic protein (SEC through IM), dipeptide transport DppA	-	-	No	Protein	Overexpressed in bacterial strain with enhanced periplasmic folding capacity	G	Periplasmic	8.200861599022275	4.111257047619937	-	-	Lennon 2015
Chaperones in the cell envelope	LOLB_ECOLI P61320	lolB	Outer-membrane lipoprotein LolB	OM lipoprotein (SEC through IM), inserted in OM by LOL), Outer-LolB	-	-	-	Protein	Incorporates lipoproteins, released from LolA, into the outer membrane	I	Outer Membrane Lipoprotein	0.42511454297849405	0.42400002530552916	-	-	Okuda 2009
Chaperones in the cell envelope	OPPA_ECOLI P23843	oppA	Periplasmic oligopeptide-binding protein	periplasmic protein (SEC through IM), oligopeptide-binding protein OppA	-	-	No	Protein	Interacts with unfolded/denatured proteins. Overexpressed in bacterial strain with enhanced periplasmic folding capacity	G	Periplasmic	9.556489584574127	11.098575003986056	-	-	Lennon 2015; Richarme 1997
Chaperones in the cell envelope	FKBA_ECOLI P45523	fkpA	FKBP-type peptidyl-prolyl cis-trans isomerase FkpA (PPIase) (EC 5.2.1.8) (Rotamase)	periplasmic protein (SEC through IM), FKBP-type peptidyl-prolyl cis-trans isomerase FkpA	-	-	No	Protein	Assists protein folding in the cell envelope via its PPiase and chaperone activity	G	Periplasmic	-	-	-	-	De Geyter 2016
Chaperones in the cell envelope	BAMA_ECOLI P0A940	bamA	Outer membrane protein assembly factor BamA (Omp85)	OM b-barrel protein (SEC through IM, inserted in OM by BAM), assembly factor BamA	-	-	-	Protein	POTRA domains of BamA recruit and assist in the folding of outer membrane protein	H	Outer Membrane b-barrel protein	3.36904641231776	5.019756088591476	-	-	Kim 2007; Knowles 2008; Sánchez-Pulido 2013;; Zhang 2011
Chaperones in the cell envelope	SKP_ECOLI P0AEU7	skp	Chaperone protein Skp (DNA-binding 17 kDa protein) (Histone-like protein HLP-1) (Seventeen kilodalton protein)	periplasmic protein (SEC through IM), Chaperone Skp	-	-	No	Protein	Assists outer membrane proteins in crossing the periplasm and folding in the outer membrane	G	Periplasmic	13.655994566173096	22.00142887217872	-	-	Bulieris 2003; Chen 1996; Harms 2001; Schaefer 1999; de Cock 1999
Chaperones in the cell envelope	LOLA_ECOLI P61316	lolA	Outer-membrane lipoprotein carrier protein (P20)	periplasmic protein (SEC through IM), Outer-carrier LolA	-	-	No	Protein	Translocates lipoproteins from the inner to the outer membrane	G	Periplasmic	1.1892136083287914	2.299987887284814	-	-	Okuda 2009
Chaperones in the cell envelope	DEGP_ECOLI P0C0V0	degP	Periplasmic serine endoprotease DegP (EC 3.4.21.107) (Heat shock protein DegP) (Protease Do)	periplasmic protein (SEC through IM), serine endoprotease DegP	-	-	-	Protein	Chaperone at low temperatures: folds outer membrane proteins, peptidase at higher temperatures: degrades denatured/misfolded/unfolded proteins in the periplasm	G	Periplasmic	5.542881289038737	4.294204462871657	-	-	Krojer 2008; Spiess 1999
Chaperones in the cell envelope	SURA_ECOLI P0ABZ6	surA	Chaperone SurA (Peptidyl-prolyl cis-trans isomerase SurA) (PPIase SurA) (EC 5.2.1.8) (Rotamase SurA) (Survival protein A)	periplasmic protein (SEC through IM), Chaperone SurA	-	-	No	Protein	Folds and assembles outer membrane proteins	G	Periplasmic	-	-	-	-	Rouviere 1996
Chaperones in the cell envelope	DJLA_ECOLI P31680	djlA	Co-chaperone protein DjlA (DnaJ-like protein DjlA)	integral IM protein (SEC in IM), Co-chaperone DjlA	-	-	-	Protein	Interacts with DnaK to induce the wcaABCDE operon	B	Integral Inner Membrane, cytoplasmic side	0.0016805942872791513	0.00005825310815277878	-	-	Chen 2001; Genevaux 2001; Kelley 1997
Chaperones in the cell envelope	PPID_ECOLI P0ADY1	ppiD	Peptidyl-prolyl cis-trans isomerase D (PPIase D) (EC 5.2.1.8) (Rotamase D)	integral IM protein (SEC in IM), Peptidyl-prolyl cis-trans isomerase D PpiD	-	-	-	Protein	Accelerates protein folding in the cell envelope	B	Integral Inner Membrane, cytoplasmic side	-	-	-	-	Antonoaea 2008
Chaperones in the cell envelope	OSMY_ECOLI P0AFH8	osmY	Osmotically-inducible protein Y	periplasmic & extra-cellular protein (SEC through IM), Osmotically-inducible Y OsmY	-	-	No	Protein	Overexpressed in bacterial strain with enhanced periplasmic folding capacity	G,X	Periplasmic, Extra-cellular	17.41854580052776	8.408567259943974	-	-	Lennon 2015
Chaperones in the cell envelope	KBAZ_ECOLI P0C8K0	kbaZ	D-tagatose-1,6-bisphosphate aldolase subunit KbaZ	cytoplasmic protein, D-tagatose-1,6-bisphosphate aldolase subunit KbaZ	-	-	-	Protein	Component of the tagatose-1,6-bisphosphate aldolase KbaYZ. KbaZ could have chaperone-like function on KbaY	A	Cytoplasmic	0.009713798840916638	0.015096996660577879	-	-	-
Chaperones in the cell envelope	KDPC_ECOLI P03961	kdpC	Potassium-transporting ATPase KdpC subunit (ATP phosphohydrolase [potassium-transporting] C chain) (Potassium-binding and translocating subunit C) (Potassium-translocating ATPase C chain)	integral IM protein (SEC in IM), Potassium-transporting ATPase KdpC subunit KdpC	-	-	-	Protein	Increases ATP-binding affinity of KdpB in the high-affinity ATP-driven potassium transport (or Kdp) system	B	Integral Inner Membrane, cytoplasmic side	-	-	-	-	Damnjanovic 2013; Irzik 2011; Kollmann 1993; Siebers 1988
Chaperones in the cell envelope	IVY_ECOLI P0AD59	ivy	Inhibitor of vertebrate lysozyme	periplasmic protein (SEC through IM), Inhibitor of vertebrate lysozyme Ivy	-	-	No	Protein	Overexpressed in bacterial strain with enhanced periplasmic folding capacity	G	Periplasmic	2.8302150816539746	1.958958207565092	-	-	Lennon 2015
Chaperones in the cell envelope	GATZ_ECOLI P0C8J8	gatZ	D-tagatose-1,6-bisphosphate aldolase subunit GatZ	cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZ	-	-	-	Protein	Component of the tagatose-1,6-bisphosphate aldolase GatYZ. GatZ could have chaperone-like function on GatY	A,F1	Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm	2.3465858995938875	20.457994947271537	-	-	Brinkkoetter 2002; Nobelmann 19996
Chaperones with role in secretion: Sec	YIDC_ECOLI P25714	yidC	Membrane protein insertase YidC (Foldase YidC) (Inner membrane protein YidC) (Membrane integrase YidC) (Oxa1Ec)	integral IM protein (SEC in IM), insertase YidC	-	-	-	Protein	Helps LacY and MalF (Sec-dependent substrates) acquire their tertiary structure	B	Integral Inner Membrane, cytoplasmic side	1.397686083067173	3.3710709667564	-	-	Nagamori 2004; Wagner 2008
Chaperones with role in secretion: Sec	SECA_ECOLI P10408	secA	Protein translocase subunit SecA	cytoplasmic peripheral IM protein, translocase subunit SecA	-	-	-	Protein	ATPase motor that drives preprotein translocation through the SecYEG channel with foldase and holdase activity	A,F1	Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm	1.1729045637525255	2.9786169750881073	1,96-3	3,15-7	Chatzi 2013; Eser 2003
Chaperones with role in secretion: Sec	SECB_ECOLI P0AG86	secB	Protein-export protein SecB (Chaperone SecB)	cytoplasmic peripheral IM protein, Protein-export SecB	-	Holdase	-	Protein	Prevents preprotein folding/aggregation and guides preproteins to the membrane receptor SecA	A,F1	Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm	27.79024830481191	46.34843575614366	0.0008	0.08	Baars 2006; Huang 2016
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The chaperonome list is available in the downloads section:
Chaperonome .xlsx format or Chaperonome .csv format

For more information: Loos et al., 2019.